Mercurial > repos > yating-l > jbrowse_hub

annotate TrackHub.py @ 15:8627394693c6 draft

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planemo upload for repository https://github.com/Yating-L/jbrowse_hub commit f18ea51d27ec7addfa6413716391cfefebc8acbc-dirty
author yating-l
date Tue, 14 Mar 2017 14:57:39 -0400
parents e1f188b43750
children 49841ae99869
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yating-l
parents:
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1 #!/usr/bin/env python
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2
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3 import os
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yating-l
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4 import trackObject
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yating-l
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5 import utils
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6 import subprocess
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7 import string
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8 import shutil
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yating-l
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9 import tempfile
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yating-l
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10
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11 #TODO: package JBrowse file conversion .pl files
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12
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13 class TrackHub:
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yating-l
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14 def __init__(self, inputFiles, reference, outputDirect, tool_dir, genome, extra_files_path):
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yating-l
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15 self.input_files = inputFiles.tracks
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yating-l
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16 self.outfile = outputDirect
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17 self.outfolder = extra_files_path
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18 self.out_path = os.path.join(extra_files_path, genome)
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19 self.reference = reference
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20 self.tool_dir = tool_dir
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21 self.raw = os.path.join(self.out_path, 'raw')
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22 self.json = os.path.join(self.out_path, 'json')
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23 try:
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24 if not self.out_path:
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25 raise ValueError('empty output path\n')
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26 if not os.path.exists(self.out_path):
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27 raise ValueError('the output folder has not been created')
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28 if os.path.exists(self.json):
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29 shutil.rmtree(self.json)
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30 os.makedirs(self.json)
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31 except OSError as e:
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32 print "Cannot create json folder error({0}): {1}".format(e.errno, e.strerror)
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yating-l
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33 else:
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34 print "Create jbrowse folder {}".format(self.out_path)
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35
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36 def createHub(self):
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37 self.prepareRefseq()
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38 for input_file in self.input_files:
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39 self.addTrack(input_file)
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40 self.indexName()
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41 self.makeArchive()
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yating-l
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42 #shutil.rmtree(self.out_path)
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43 self.outHtml()
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44 print "Success!\n"
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45
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46 def prepareRefseq(self):
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47 try:
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48 #print os.path.join(self.tool_dir, 'prepare-refseqs.pl') + ", '--fasta', " + self.reference +", '--out', self.json])"
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49 p = subprocess.Popen(['prepare-refseqs.pl', '--fasta', self.reference, '--out', self.json])
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50 # Wait for process to terminate.
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51 p.communicate()
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52 except OSError as e:
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53 print "Cannot prepare reference error({0}): {1}".format(e.errno, e.strerror)
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yating-l
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54 #TODO: hard coded the bam and bigwig tracks. Need to allow users to customize the settings
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55 def addTrack(self, track):
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56 if track['dataType'] == 'bam':
0
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57 json_file = os.path.join(self.json, "trackList.json")
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58 bam_track = dict()
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59 bam_track['type'] = 'JBrowse/View/Track/Alignments2'
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60 bam_track['label'] = track.fileName
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61 bam_track['urlTemplate'] = os.path.join('../raw', track.fileName)
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62 utils.add_tracks_to_json(json_file, bam_track, 'add_tracks')
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63 print "add bam track\n"
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64 elif track['dataType'] == 'bigwig':
0
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65 json_file = os.path.join(self.json, "trackList.json")
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66 bigwig_track = dict()
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67 bigwig_track['label'] = 'rnaseq'
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68 bigwig_track['key'] = 'RNA-Seq Coverage'
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69 bigwig_track['urlTemplate'] = os.path.join('../raw', track.fileName)
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70 bigwig_track['type'] = 'JBrowse/View/Track/Wiggle/XYPlot'
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71 bigwig_track['variance_band'] = True
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72 bigwig_track['style'] = dict()
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73 bigwig_track['style']['pos_color'] = '#FFA600'
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74 bigwig_track['style']['neg_color'] = '#005EFF'
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75 bigwig_track['style']['clip_marker_color'] = 'red'
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76 bigwig_track['style']['height'] = 100
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77 utils.add_tracks_to_json(json_file, bigwig_track, 'add_tracks')
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78 else:
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79 gff3_file = os.path.join(self.raw, track['fileName'])
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80 label = track['fileName']
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81 if track['dataType'] == 'bedSpliceJunctions' or track['dataType'] == 'gtf':
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yating-l
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82 p = subprocess.Popen(['flatfile-to-json.pl', '--gff', gff3_file, '--trackType', 'CanvasFeatures', '--trackLabel', label, '--config', '{"glyph": "JBrowse/View/FeatureGlyph/Segments"}', '--out', self.json])
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83 elif track['dataType'] == 'gff3_transcript':
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84 p = subprocess.Popen(['flatfile-to-json.pl', '--gff', gff3_file, '--trackType', 'MyPlugin/GenePred', '--trackLabel', label, '--config', '{"transcriptType": "transcript"}', '--out', self.json])
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85 elif track['dataType'] == 'gff3_mrna':
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86 p = subprocess.Popen(['flatfile-to-json.pl', '--gff', gff3_file, '--trackType', 'MyPlugin/GenePred', '--trackLabel', label, '--out', self.json])
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87 else:
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88 p = subprocess.Popen(['flatfile-to-json.pl', '--gff', gff3_file, '--trackType', 'CanvasFeatures', '--trackLabel', label, '--out', self.json])
0
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89 p.communicate()
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90
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91 def indexName(self):
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92 p = subprocess.Popen(['generate-names.pl', '-v', '--out', self.json])
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93 p.communicate()
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94 print "finished name index \n"
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96 def makeArchive(self):
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97 shutil.make_archive(self.out_path, 'zip', self.out_path)
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98 shutil.rmtree(self.out_path)
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99
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100 #TODO: this will list all zip files in the filedir and sub-dirs. worked in Galaxy but all list zip files in test-data when
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101 #run it locally. May need modify
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102 def outHtml(self):
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103 #htmloutput = tempfile.NamedTemporaryFile(self.outfile, suffix = '.html', bufsize=0, delete=False)
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104 with open(self.outfile, 'w') as htmlfile:
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105 htmlstr = 'The JBrowse Hub is created: <br>'
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106 zipfiles = '<li><a href = "%s">Download</a></li>'
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107 filedir_abs = os.path.abspath(self.outfile)
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108 filedir = os.path.dirname(filedir_abs)
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109 filedir = os.path.join(filedir, self.outfolder)
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110 for root, dirs, files in os.walk(filedir):
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111 for file in files:
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112 if file.endswith('.zip'):
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113 relative_directory = os.path.relpath(root, filedir)
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114 relative_file_path = os.path.join(relative_directory, file)
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115 htmlstr += zipfiles % relative_file_path
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116
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117 #htmlstr = htmlstr % zipfile
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118 htmlfile.write(htmlstr)
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119
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