Mercurial > repos > peterjc > rxlr_venn_workflow
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author | peterjc |
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date | Wed, 21 Aug 2013 13:00:43 -0400 |
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1 This is package is a Galaxy workflow for comparing three RXLR prediction |
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2 methods with a Venn Diagram, and creates a FASTA file of any proteins |
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3 passing all three methods. |
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4 |
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5 See http://www.galaxyproject.org for information about the Galaxy Project. |
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6 |
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7 |
7 | 8 Sample Data |
9 =========== | |
10 | |
11 This workflow was developed and run on several Phytophthora species. | |
12 For example, try the "Phyca11" protein set for Phytophthora capsici: | |
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14 http://genome.jgi-psf.org/Phyca11/download/Phyca11_filtered_proteins.fasta.gz | |
15 | |
16 You can upload this directly into Galaxy via this URL. Galaxy will handle | |
17 removing the gzip compression to give you the FASTA protein file which | |
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18 has 19,805 protein sequences. The expected results: |
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19 |
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20 * 89 RXLRs using Whisson et al. (2007) |
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21 * 124 RXLRs using Win et al. (2007) |
11 | 22 * 162 RXLRs using Bhattacharjee et al. (2006) |
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23 |
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24 Of these, only 79 sequences pass all three of the RXLR prediction tools, |
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25 while 19643 have no RXLR matches at all. |
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26 |
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27 .. image:: https://raw.github.com/peterjc/picobio/master/galaxy_workflows/rxlr_venn_workflow/Phyca11_example_output.png |
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28 :height: 400px |
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29 :width: 400px |
7 | 30 |
31 | |
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32 Citation |
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33 ======== |
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34 |
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35 If you use this workflow directly, or a derivative of it, in work leading |
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36 to a scientific publication, please cite: |
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37 |
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38 Cock, P.J.A. and Pritchard, L. 2013. Galaxy as a platform for identifying |
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39 candidate pathogen effectors. Chapter 1 in "Plant-Pathogen Interactions: |
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40 Methods and Protocols (Second Edition)"; Methods in Molecular Biology. |
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41 Humana Press, Springer. In press. |
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42 |
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43 Whisson, S.C., Boevink, C.V., Moleleki, L., et al. (2007) |
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44 A translocation signal for delivery of oomycete effector proteins into |
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45 host plant cells. Nature 450:115-118. |
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46 http://dx.doi.org/10.1038/nature06203 |
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47 |
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48 Win, J., Morgan, W., Bos, J., et al. (2007) |
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49 Adaptive evolution has targeted the C-terminal domain of the RXLR effectors |
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50 of plant pathogenic oomycetes. The Plant Cell 19:2349-2369. |
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51 http://dx.doi.org/10.1105/tpc.107.051037 |
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52 |
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53 Bhattacharjee, S., Luisa Hiller, N., Liolios, K., et al. (2006) |
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54 The malarial host-targeting signal is conserved in the Irish potato famine |
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55 pathogen. PLoS Pathogens 2(5):e50. |
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56 http://dx.doi.org/10.1371/journal.ppat.0020050 |
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57 |
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58 |
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59 Availability |
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60 ============ |
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61 |
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62 This workflow is available to download and/or install from the main |
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63 Galaxy Tool Shed: |
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64 |
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65 http://toolshed.g2.bx.psu.edu/view/peterjc/rxlr_venn_workflow |
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66 |
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67 Test releases (which should not normally be used) are on the Test Tool Shed: |
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68 |
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69 http://testtoolshed.g2.bx.psu.edu/view/peterjc/rxlr_venn_workflow |
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70 |
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71 Development is being done on github here: |
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72 |
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73 https://github.com/peterjc/picobio/tree/master/galaxy_workflows/rxlr_venn_workflow |
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74 |
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75 |
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76 Dependencies |
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77 ============ |
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78 |
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79 These dependencies should be resolved automatically via the Galaxy Tool Shed: |
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80 |
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81 * http://toolshed.g2.bx.psu.edu/view/peterjc/tmhmm_and_signalp |
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82 * http://toolshed.g2.bx.psu.edu/view/peterjc/seq_filter_by_id |
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83 * http://toolshed.g2.bx.psu.edu/view/peterjc/venn_list |
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84 |
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85 However, at the time of writing those Galaxy tools have their own dependencies |
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86 required for this workflow which require manual installation (SignalP v3.0, |
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87 HMMER v2.0, and the R/Bioconductor package limma). |
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88 |
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89 |
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90 History |
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91 ======= |
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92 |
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93 ======= ====================================================================== |
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94 Version Changes |
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95 ------- ---------------------------------------------------------------------- |
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96 v0.0.1 - Initial release to Tool Shed (May, 2013) |
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97 - Expanded README file to include example data |
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98 v0.0.2 - Updated versions of the tools used, inclulding core Galaxy Filter |
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99 tool to avoid warning about new ``header_lines`` parameter. |
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100 - Added link to Tool Shed in the workflow annotation explaining there |
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101 is a README file with sample data, and a requested citation. |
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102 ======= ====================================================================== |
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103 |
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104 |
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105 Developers |
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106 ========== |
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107 |
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108 This workflow is under source code control here: |
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109 |
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110 https://github.com/peterjc/picobio/tree/master/galaxy_workflows/rxlr_venn_workflow |
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111 |
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112 To prepare the tar-ball for uploading to the Tool Shed, I use this: |
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113 |
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114 $ tar -cf rxlr_venn_workflow.tar.gz README.rst repository_dependencies.xml rxlr_venn_workflow.ga |
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115 |
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116 Check this, |
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117 |
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118 $ tar -tzf rxlr_venn_workflow.tar.gz |
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119 README.rst |
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120 repository_dependencies.xml |
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121 rxlr_venn_workflow.ga |