Mercurial > repos > peterjc > rxlr_venn_workflow
annotate README.rst @ 7:51aeb3d4799d draft
Uploaded v0.0.1g, add Phyca11 example to README
author | peterjc |
---|---|
date | Mon, 19 Aug 2013 12:31:11 -0400 |
parents | b1047023ffbb |
children | 2dfe8b788fb6 |
rev | line source |
---|---|
6
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
1 This is package is a Galaxy workflow for comparing three RXLR prediction |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
2 methods with a Venn Diagram, and creates a FASTA file of any proteins |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
3 passing all three methods. |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
4 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
5 See http://www.galaxyproject.org for information about the Galaxy Project. |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
6 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
7 |
7 | 8 Sample Data |
9 =========== | |
10 | |
11 This workflow was developed and run on several Phytophthora species. | |
12 For example, try the "Phyca11" protein set for Phytophthora capsici: | |
13 | |
14 http://genome.jgi-psf.org/Phyca11/download/Phyca11_filtered_proteins.fasta.gz | |
15 | |
16 You can upload this directly into Galaxy via this URL. Galaxy will handle | |
17 removing the gzip compression to give you the FASTA protein file which | |
18 has 19,805 protein sequences. Of these, only 79 sequences pass all three | |
19 of the RXLR prediction tools. | |
20 | |
21 | |
6
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
22 Citation |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
23 ======== |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
24 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
25 If you use this workflow directly, or a derivative of it, in work leading |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
26 to a scientific publication, please cite: |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
27 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
28 Cock, P.J.A. and Pritchard, L. 2013. Galaxy as a platform for identifying |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
29 candidate pathogen effectors. Chapter 1 in "Plant-Pathogen Interactions: |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
30 Methods and Protocols (Second Edition)"; Methods in Molecular Biology. |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
31 Humana Press, Springer. In press. |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
32 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
33 Whisson, S.C., Boevink, C.V., Moleleki, L., et al. (2007) |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
34 A translocation signal for delivery of oomycete effector proteins into |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
35 host plant cells. Nature 450:115-118. |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
36 http://dx.doi.org/10.1038/nature06203 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
37 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
38 Win, J., Morgan, W., Bos, J., et al. (2007) |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
39 Adaptive evolution has targeted the C-terminal domain of the RXLR effectors |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
40 of plant pathogenic oomycetes. The Plant Cell 19:2349-2369. |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
41 http://dx.doi.org/10.1105/tpc.107.051037 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
42 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
43 Bhattacharjee, S., Luisa Hiller, N., Liolios, K., et al. (2006) |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
44 The malarial host-targeting signal is conserved in the Irish potato famine |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
45 pathogen. PLoS Pathogens 2(5):e50. |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
46 http://dx.doi.org/10.1371/journal.ppat.0020050 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
47 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
48 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
49 Availability |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
50 ============ |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
51 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
52 This workflow is available to download and/or install from the main |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
53 Galaxy Tool Shed: |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
54 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
55 http://toolshed.g2.bx.psu.edu/view/peterjc/rxlr_venn_workflow |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
56 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
57 Test releases (which should not normally be used) are on the Test Tool Shed: |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
58 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
59 http://testtoolshed.g2.bx.psu.edu/view/peterjc/rxlr_venn_workflow |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
60 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
61 Development is being done on github here: |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
62 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
63 https://github.com/peterjc/picobio/tree/master/galaxy_workflows/rxlr_venn_workflow |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
64 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
65 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
66 Dependencies |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
67 ============ |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
68 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
69 These dependencies should be resolved automatically via the Galaxy Tool Shed: |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
70 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
71 * http://toolshed.g2.bx.psu.edu/view/peterjc/tmhmm_and_signalp |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
72 * http://toolshed.g2.bx.psu.edu/view/peterjc/seq_filter_by_id |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
73 * http://toolshed.g2.bx.psu.edu/view/peterjc/venn_list |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
74 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
75 However, at the time of writing those Galaxy tools have their own dependencies |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
76 required for this workflow which require manual installation (SignalP v3.0, |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
77 HMMER v2.0, and the R/Bioconductor package limma). |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
78 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
79 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
80 Developers |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
81 ========== |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
82 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
83 This workflow is under source code control here: |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
84 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
85 https://github.com/peterjc/picobio/tree/master/galaxy_workflows/rxlr_venn_workflow |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
86 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
87 To prepare the tar-ball for uploading to the Tool Shed, I use this: |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
88 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
89 $ tar -cf rxlr_venn_workflow.tar.gz README.rst repository_dependencies.xml rxlr_venn_workflow.ga |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
90 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
91 Check this, |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
92 |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
93 $ tar -tzf rxlr_venn_workflow.tar.gz |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
94 README.rst |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
95 repository_dependencies.xml |
b1047023ffbb
Uploaded v0.0.1f, README file with clearer citation instructions.
peterjc
parents:
diff
changeset
|
96 rxlr_venn_workflow.ga |