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1 FALCO Amplicon Analysis Pipeline & Galaxy wrapper
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2 ========================================
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3
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4 FALCO variant-caller is part of the Amplicon Analysis Pipeline (AAP).
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5
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6 The typical workflow is as follows:
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7 * paired-end amplicon sequencing
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8 * merge pairs by overlapping them
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9 * map the single read fastq with BWA
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10 * perform variant calling with FALCO
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11 * create (html) report of the results
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12
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13 FALCO uses samtools and straight-forward statistics to determine wether a
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14 potential variant is likely a (technical) artifact or not.
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15
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16 Input / Output
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17
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18 Input of the caller is a BAM file, output VCF
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19
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20
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21 History
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22
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23 ============== ================================================================
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24 Date Changes
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25 -------------- ----------------------------------------------------------------
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26 December 2014 * first release in Test Tool Shed
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27 ============== ================================================================
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28
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29
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30 Bug Reports & other questions
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31 =============================
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32
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33 Issues can be reported via http://www.tgac.nl/
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