Mercurial > repos > stef > falco
diff README.rst @ 54:036033e74757 draft
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| author | stef |
|---|---|
| date | Wed, 31 Dec 2014 07:16:40 -0500 |
| parents | |
| children | e7de191375e6 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/README.rst Wed Dec 31 07:16:40 2014 -0500 @@ -0,0 +1,33 @@ +FALCO Amplicon Analysis Pipeline & Galaxy wrapper +======================================== + +FALCO variant-caller is part of the Amplicon Analysis Pipeline (AAP). + +The typical workflow is as follows: +* paired-end amplicon sequencing +* merge pairs by overlapping them +* map the single read fastq with BWA +* perform variant calling with FALCO +* create (html) report of the results + +FALCO uses samtools and straight-forward statistics to determine wether a +potential variant is likely a (technical) artifact or not. + +Input / Output + +Input of the caller is a BAM file, output VCF + + +History + +============== ================================================================ +Date Changes +-------------- ---------------------------------------------------------------- +December 2014 * first release in Test Tool Shed +============== ================================================================ + + +Bug Reports & other questions +============================= + +Issues can be reported via http://www.tgac.nl/
