annotate data_manager/novocraft_index_builder.py @ 11:a9b725202132 draft

planemo upload for repository https://github.com/zipho/data_manager_novocraft_index_builder commit 9ea1c9c5fdf0ed65170469f809b81ba9e82f91d3
author sanbi-uwc
date Mon, 07 Mar 2016 02:27:51 -0500
parents 68b7023c6a2f
children 283eec05d1de
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1 #!/usr/bin/env python
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2 # Z. Mashologu (SANBI-UWC)
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3 # import dict as dict
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4 from __future__ import print_function
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5 import os
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6 import sys
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7 import urllib2
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8 import logging
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9 import argparse
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10 import shlex
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11 from subprocess import check_call, CalledProcessError
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13 log = logging.getLogger(__name__)
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15 from json import loads, dumps
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17 DEFAULT_DATA_TABLE_NAME = "novocraft_index"
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19 def get_dbkey_id_name(params, dbkey_description=None):
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20 dbkey = params['param_dict']['dbkey']
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21 # TODO: ensure sequence_id is unique and does not already appear in location file
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22 sequence_id = params['param_dict']['sequence_id']
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23 if not sequence_id:
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24 sequence_id = dbkey # uuid.uuid4() generate and use an uuid instead?
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26 sequence_name = params['param_dict']['sequence_name']
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27 if not sequence_name:
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28 sequence_name = dbkey_description
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29 if not sequence_name:
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30 sequence_name = dbkey
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31 return dbkey, sequence_id, sequence_name
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32
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33 def _make_novocraft_index(data_manager_dict, fasta_filename, target_directory, dbkey, sequence_id, sequence_name, data_table_name=DEFAULT_DATA_TABLE_NAME):
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34 if os.path.exists(target_directory) and not os.path.isdir(target_directory):
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35 print("Output directory path already exists but is not a directory: {}".format(target_directory),
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36 file=sys.stderr)
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37 elif not os.path.exists(target_directory):
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38 os.mkdir(target_directory)
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39
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40 index_filename = os.path.join(target_directory,"%.nix" % sequence_id)
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41 cmdline_str = 'novoindex {} {}'.format(index_filename, fasta_filename)
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42 cmdline = shlex.split(cmdline_str)
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44 #index_filename = 'foo'
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45 #cmdline = ('touch', '{}/{}'.format(target_directory, index_filename))
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46 try:
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47 check_call(cmdline)
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48 except CalledProcessError:
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49 print("Error building RNA STAR index", file=sys.stderr)
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51 data_table_entry = dict( value=sequence_id, dbkey=dbkey, name=sequence_name, path=index_filename )
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52 _add_data_table_entry( data_manager_dict, data_table_name, data_table_entry )
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54 def _add_data_table_entry( data_manager_dict, data_table_name, data_table_entry ):
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55 data_manager_dict['data_tables'] = data_manager_dict.get( 'data_tables', {} )
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56 data_manager_dict['data_tables'][ data_table_name ] = data_manager_dict['data_tables'].get( data_table_name, [] )
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57 data_manager_dict['data_tables'][ data_table_name ].append( data_table_entry )
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58 return data_manager_dict
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60 def download_from_url( data_manager_dict, params, target_directory, dbkey, sequence_id, sequence_name, data_table_name=DEFAULT_DATA_TABLE_NAME ):
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61 # TODO: we should automatically do decompression here
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62 urls = filter(bool, map(lambda x: x.strip(), params['param_dict']['reference_source']['user_url'].split('\n')))
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63 fasta_reader = [urllib2.urlopen(url) for url in urls]
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65 _make_novocraft_index(data_manager_dict, fasta_reader, target_directory, dbkey, sequence_id, sequence_name, data_table_name)
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67 def download_from_history( data_manager_dict, params, target_directory, dbkey, sequence_id, sequence_name, data_table_name=DEFAULT_DATA_TABLE_NAME ):
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68 # TODO: allow multiple FASTA input files
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69 input_filename = params['param_dict']['reference_source']['input_fasta']
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71 _make_novocraft_index(data_manager_dict, input_filename, target_directory, dbkey, sequence_id, sequence_name, data_table_name)
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73 REFERENCE_SOURCE_TO_DOWNLOAD = dict(url=download_from_url, history=download_from_history)
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75 def main():
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76 parser = argparse.ArgumentParser(description="Generate Novo-craft genome index and JSON describing this")
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77 parser.add_argument('output_filename')
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78 parser.add_argument('--dbkey_description')
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79 parser.add_argument('--data_table_name', default='novocraft_index')
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80 args = parser.parse_args()
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82 filename = args.output_filename
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84 params = loads(open(filename).read())
6cddc1a6e282 planemo upload for repository https://github.com/zipho/data_manager_novocraft_index_builder commit 3a0b36e3ee66f35f7e2d5e1220cd883a9d528fac
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85 target_directory = params['output_data'][0]['extra_files_path']
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86 os.makedirs(target_directory)
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87 data_manager_dict = {}
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6cddc1a6e282 planemo upload for repository https://github.com/zipho/data_manager_novocraft_index_builder commit 3a0b36e3ee66f35f7e2d5e1220cd883a9d528fac
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88
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89 dbkey, sequence_id, sequence_name = get_dbkey_id_name(params, dbkey_description=args.dbkey_description)
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90
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91 if dbkey in [None, '', '?']:
6cddc1a6e282 planemo upload for repository https://github.com/zipho/data_manager_novocraft_index_builder commit 3a0b36e3ee66f35f7e2d5e1220cd883a9d528fac
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92 raise Exception('"%s" is not a valid dbkey. You must specify a valid dbkey.' % (dbkey))
6cddc1a6e282 planemo upload for repository https://github.com/zipho/data_manager_novocraft_index_builder commit 3a0b36e3ee66f35f7e2d5e1220cd883a9d528fac
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94 # Fetch the FASTA
6cddc1a6e282 planemo upload for repository https://github.com/zipho/data_manager_novocraft_index_builder commit 3a0b36e3ee66f35f7e2d5e1220cd883a9d528fac
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95 REFERENCE_SOURCE_TO_DOWNLOAD[params['param_dict']['reference_source']['reference_source_selector']]\
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96 (data_manager_dict, params, target_directory, dbkey, sequence_id, sequence_name, data_table_name=args.data_table_name or DEFAULT_DATA_TABLE_NAME)
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97
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98 open(filename, 'wb').write(dumps( data_manager_dict ))
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99
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100 if __name__ == "__main__": main()