Mercurial > repos > matthias > stacks2_tsv2bam
annotate stacks_tsv2bam.xml @ 6:2626e58c493e draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 82e7263f3e57a29362d981e36b82b82063f12ac3
author | matthias |
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date | Tue, 25 Jun 2019 10:58:48 -0400 |
parents | 852044374da1 |
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60c1c4d7d2c1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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1 <tool id="stacks2_tsv2bam" name="Stacks2: tsv2bam" profile="@PROFILE@" version="@STACKS_VERSION@+galaxy@WRAPPER_VERSION@"> |
60c1c4d7d2c1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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2 <description>Sort reads by RAD locus</description> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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3 <macros> |
99d0b19ef534
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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4 <import>macros.xml</import> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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5 </macros> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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6 <expand macro="requirements"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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7 <expand macro="version_cmd"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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8 <command detect_errors="aggressive"><![CDATA[ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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9 @FASTQ_INPUT_FUNCTIONS@ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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10 |
99d0b19ef534
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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11 mkdir stacks_inputs stacks_outputs && |
99d0b19ef534
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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12 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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13 @LINK_STACKS_INPUT@ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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14 @LINK_CAT_INPUT@ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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15 @LINK_MATCHES_INPUT@ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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16 |
99d0b19ef534
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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17 ## link fastq files into stacks_inputs/ |
99d0b19ef534
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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18 #set $paired_reads=False |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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19 #for $sample in $input_type.fqinputs: |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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20 #if not $sample: |
99d0b19ef534
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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21 #continue |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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22 #end if |
99d0b19ef534
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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23 #set $paired_reads=True |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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24 #set ($link_cmd, $data_path, $name, $inputype) = $fastq_input_foo($sample, "reverse", ".2") |
60c1c4d7d2c1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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25 $link_cmd |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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26 #end for |
99d0b19ef534
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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27 |
99d0b19ef534
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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28 tsv2bam |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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29 -P stacks_inputs |
99d0b19ef534
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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30 #if $popmap |
99d0b19ef534
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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31 -M '$popmap' |
99d0b19ef534
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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32 #else |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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33 #for $sample in $samples |
60c1c4d7d2c1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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34 -s '$sample' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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35 #end for |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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36 #end if |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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37 #if $paired_reads |
99d0b19ef534
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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38 --pe-reads-dir stacks_inputs |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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39 #end if |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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40 -t \${GALAXY_SLOTS:-1} |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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41 ## tsv2bam writes to stdout and creates a log file |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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42 ## - make stdout stderr |
99d0b19ef534
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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43 ## - move the additional log file |
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44 2>&1 |
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45 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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46 #if $output_log |
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47 && mv stacks_inputs/tsv2bam.log $output_log |
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48 #end if |
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49 && mv stacks_inputs/*matches.bam stacks_outputs |
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50 ]]></command> |
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51 |
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52 <inputs> |
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53 <expand macro="input_stacks_macro"/> |
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54 <expand macro="input_cat_macro"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit c42d129928cfd153ba7a922368c8e76e79b242b2
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55 <expand macro="input_matches_macro"/> |
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56 <!-- TODO add BAM? --> |
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57 <expand macro="fastq_input" fastq_optional="true" se_option="reverse reads" help="Paired end data or reverse reads. If a paired list is provided only the reverse reads are used in tsv2bam. Leave selection empty if you analyse single end data."/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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58 <param name="popmap" type="data" format="tabular,txt" label="Population map" help="If set, matching will be done only for samples listed in this file" optional="true" argument="-M" /> |
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59 <expand macro="in_log"/> |
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60 </inputs> |
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61 |
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62 <outputs> |
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63 <expand macro="out_log"/> |
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64 <expand macro="tsv2bam_outputs_macro"/> |
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65 </outputs> |
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66 |
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67 <tests> |
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68 <!-- test wo paired end data --> |
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69 <test> |
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70 <param name="input_cat"> |
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71 <collection type="list"> |
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72 <element name="catalog.alleles" ftype="tabular" value="cstacks/catalog.alleles.tsv" /> |
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73 <element name="catalog.snps" ftype="tabular" value="cstacks/catalog.snps.tsv" /> |
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74 <element name="catalog.tags" ftype="tabular" value="cstacks/catalog.tags.tsv" /> |
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75 </collection> |
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76 </param> |
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77 <param name="input_stacks"> |
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78 <collection type="list"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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79 <element name="PopA_01.alleles" ftype="tabular" value="ustacks/PopA_01.alleles.tsv" /> |
60c1c4d7d2c1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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80 <element name="PopA_01.snps" ftype="tabular" value="ustacks/PopA_01.snps.tsv" /> |
60c1c4d7d2c1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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81 <element name="PopA_01.tags" ftype="tabular" value="ustacks/PopA_01.tags.tsv" /> |
60c1c4d7d2c1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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82 <element name="PopA_02.alleles" ftype="tabular" value="ustacks/PopA_02.alleles.tsv" /> |
60c1c4d7d2c1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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83 <element name="PopA_02.snps" ftype="tabular" value="ustacks/PopA_02.snps.tsv" /> |
60c1c4d7d2c1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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84 <element name="PopA_02.tags" ftype="tabular" value="ustacks/PopA_02.tags.tsv" /> |
0
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85 </collection> |
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86 </param> |
99d0b19ef534
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87 <param name="input_matches"> |
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88 <collection type="list"> |
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89 <element name="PopA_01.matches" ftype="tabular" value="sstacks/PopA_01.matches.tsv" /> |
99d0b19ef534
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 98327d2948ae1ccb5aef5db9ab88605fd74a0de7-dirty
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90 <element name="PopA_02.matches" ftype="tabular" value="sstacks/PopA_02.matches.tsv" /> |
99d0b19ef534
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91 </collection> |
99d0b19ef534
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92 </param> |
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93 <param name="add_log" value="yes" /> |
5
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94 <output name="output_log" ftype="txt" file="tsv2bam/tsv2bam.log" lines_diff="14"/> |
0
99d0b19ef534
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95 <output_collection name="bams" type="list" count="2"> |
4
60c1c4d7d2c1
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 4e87a14a5479800df9675c1cbcdbe1b11f63653b-dirty
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96 <element name="PopA_01.matches" file="tsv2bam/PopA_01.matches.bam" ftype="bam"/> |
60c1c4d7d2c1
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97 <element name="PopA_02.matches" file="tsv2bam/PopA_02.matches.bam" ftype="bam"/> |
0
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98 </output_collection> |
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99 </test> |
99d0b19ef534
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100 <!-- test w popmap, w reverse reads as multiple selection --> |
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101 <test> |
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102 <param name="input_cat"> |
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103 <collection type="list"> |
4
60c1c4d7d2c1
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104 <element name="catalog.alleles" ftype="tabular" value="cstacks/catalog.alleles.tsv" /> |
60c1c4d7d2c1
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105 <element name="catalog.snps" ftype="tabular" value="cstacks/catalog.snps.tsv" /> |
60c1c4d7d2c1
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106 <element name="catalog.tags" ftype="tabular" value="cstacks/catalog.tags.tsv" /> |
0
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107 </collection> |
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108 </param> |
4
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109 <param name="input_stacks"> |
0
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110 <collection type="list"> |
4
60c1c4d7d2c1
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111 <element name="PopA_01.alleles" ftype="tabular" value="ustacks/PopA_01.alleles.tsv" /> |
60c1c4d7d2c1
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112 <element name="PopA_01.snps" ftype="tabular" value="ustacks/PopA_01.snps.tsv" /> |
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113 <element name="PopA_01.tags" ftype="tabular" value="ustacks/PopA_01.tags.tsv" /> |
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114 <element name="PopA_02.alleles" ftype="tabular" value="ustacks/PopA_02.alleles.tsv" /> |
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115 <element name="PopA_02.snps" ftype="tabular" value="ustacks/PopA_02.snps.tsv" /> |
60c1c4d7d2c1
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116 <element name="PopA_02.tags" ftype="tabular" value="ustacks/PopA_02.tags.tsv" /> |
0
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117 </collection> |
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118 </param> |
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119 <param name="input_matches"> |
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120 <collection type="list"> |
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121 <element name="PopA_01.matches" ftype="tabular" value="sstacks/PopA_01.matches.tsv" /> |
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122 <element name="PopA_02.matches" ftype="tabular" value="sstacks/PopA_02.matches.tsv" /> |
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123 </collection> |
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124 </param> |
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125 <param name="popmap" ftype="tabular" value="denovo_map/popmap_cstacks.tsv" /> |
4
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126 <param name="input_type|input_type_select" value="single"/> |
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127 <param name="input_type|fqinputs" value="demultiplexed/PopA_01.2.fq,demultiplexed/PopA_02.2.fq" ftype="fastqsanger" /> |
0
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128 <param name="add_log" value="yes" /> |
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129 <output name="output_log"><assert_contents><has_text text="done." /></assert_contents></output> |
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130 <output_collection name="bams" type="list" count="2"/> |
0
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131 </test> |
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132 <!-- test w reverse reads as list --> |
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133 <test> |
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134 <param name="input_cat"> |
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135 <collection type="list"> |
4
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136 <element name="catalog.alleles" ftype="tabular" value="cstacks/catalog.alleles.tsv" /> |
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137 <element name="catalog.snps" ftype="tabular" value="cstacks/catalog.snps.tsv" /> |
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138 <element name="catalog.tags" ftype="tabular" value="cstacks/catalog.tags.tsv" /> |
0
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139 </collection> |
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140 </param> |
4
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141 <param name="input_stacks"> |
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142 <collection type="list"> |
4
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143 <element name="PopA_01.alleles" ftype="tabular" value="ustacks/PopA_01.alleles.tsv" /> |
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144 <element name="PopA_01.snps" ftype="tabular" value="ustacks/PopA_01.snps.tsv" /> |
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145 <element name="PopA_01.tags" ftype="tabular" value="ustacks/PopA_01.tags.tsv" /> |
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146 <element name="PopA_02.alleles" ftype="tabular" value="ustacks/PopA_02.alleles.tsv" /> |
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147 <element name="PopA_02.snps" ftype="tabular" value="ustacks/PopA_02.snps.tsv" /> |
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148 <element name="PopA_02.tags" ftype="tabular" value="ustacks/PopA_02.tags.tsv" /> |
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149 </collection> |
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150 </param> |
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151 <param name="input_matches"> |
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152 <collection type="list"> |
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153 <element name="PopA_01.matches" ftype="tabular" value="sstacks/PopA_01.matches.tsv" /> |
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154 <element name="PopA_02.matches" ftype="tabular" value="sstacks/PopA_02.matches.tsv" /> |
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155 </collection> |
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156 </param> |
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157 <param name="input_type|input_type_select" value="paired"/> |
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158 <param name="input_type|fqinputs"> |
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159 <collection type="list"> |
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160 <element name="PopA_01" value="demultiplexed/PopA_01.2.fq" ftype="fastqsanger" /> |
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161 <element name="PopA_02" value="demultiplexed/PopA_02.2.fq" ftype="fastqsanger"/> |
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162 </collection> |
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163 </param> |
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164 <param name="add_log" value="yes" /> |
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165 <output name="output_log"><assert_contents><has_text text="done." /></assert_contents></output> |
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166 <output_collection name="bams" type="list" count="2"/> |
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167 </test> |
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168 <!-- test w paired reads as paired dataset list --> |
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169 <test> |
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170 <param name="input_cat"> |
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171 <collection type="list"> |
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172 <element name="catalog.alleles" ftype="tabular" value="cstacks/catalog.alleles.tsv" /> |
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173 <element name="catalog.snps" ftype="tabular" value="cstacks/catalog.snps.tsv" /> |
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174 <element name="catalog.tags" ftype="tabular" value="cstacks/catalog.tags.tsv" /> |
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175 </collection> |
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176 </param> |
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177 <param name="input_stacks"> |
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178 <collection type="list"> |
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179 <element name="PopA_01.alleles" ftype="tabular" value="ustacks/PopA_01.alleles.tsv" /> |
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180 <element name="PopA_01.snps" ftype="tabular" value="ustacks/PopA_01.snps.tsv" /> |
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181 <element name="PopA_01.tags" ftype="tabular" value="ustacks/PopA_01.tags.tsv" /> |
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182 <element name="PopA_02.alleles" ftype="tabular" value="ustacks/PopA_02.alleles.tsv" /> |
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183 <element name="PopA_02.snps" ftype="tabular" value="ustacks/PopA_02.snps.tsv" /> |
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184 <element name="PopA_02.tags" ftype="tabular" value="ustacks/PopA_02.tags.tsv" /> |
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185 </collection> |
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186 </param> |
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187 <param name="input_matches"> |
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188 <collection type="list"> |
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189 <element name="PopA_01.matches" ftype="tabular" value="sstacks/PopA_01.matches.tsv" /> |
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190 <element name="PopA_02.matches" ftype="tabular" value="sstacks/PopA_02.matches.tsv" /> |
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191 </collection> |
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192 </param> |
4
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193 <param name="input_type|input_type_select" value="paired"/> |
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194 <param name="input_type|fqinputs"> |
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195 <collection type="list:paired"> |
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196 <element name="PopA_01"> |
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197 <collection type="paired"> |
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198 <element name="forward" value="demultiplexed/PopA_01.1.fq" ftype="fastqsanger" /> |
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199 <element name="reverse" value="demultiplexed/PopA_01.2.fq" ftype="fastqsanger"/> |
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200 </collection> |
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201 </element> |
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202 <element name="PopA_02"> |
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203 <collection type="paired"> |
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204 <element name="forward" value="demultiplexed/PopA_02.1.fq" ftype="fastqsanger" /> |
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205 <element name="reverse" value="demultiplexed/PopA_02.2.fq" ftype="fastqsanger"/> |
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206 </collection> |
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207 </element> |
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208 </collection> |
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209 </param> |
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210 <param name="add_log" value="yes" /> |
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211 <output name="output_log"><assert_contents><has_text text="done." /></assert_contents></output> |
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212 <output_collection name="bams" type="list" count="2"/> |
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213 </test> |
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214 </tests> |
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215 |
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216 <help> |
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217 <![CDATA[ |
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218 .. class:: infomark |
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219 |
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220 **What it does** |
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221 |
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222 The unique stacks program will take as input a set of short-read sequences and align them into exactly-matching stacks. Comparing the stacks it will form a set of loci and detect SNPs at each locus using a maximum likelihood framework |
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223 |
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224 -------- |
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225 |
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226 **Input files** |
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227 |
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228 FASTQ, FASTA |
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229 |
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230 **Output files** |
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231 |
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232 - XXX.tags.tsv file: |
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233 |
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234 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_ |
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235 |
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236 Notes: For the tags file, each stack will start in the file with a consensus sequence for the entire stack followed by the flags for that stack. Then, each individual read that was merged into that stack will follow. The next stack will start with another consensus sequence. |
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237 |
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238 |
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239 - XXX.snps.tsv file: |
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240 |
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241 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_ |
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242 |
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243 Notes: If a stack has two SNPs called within it, then there will be two lines in this file listing each one. |
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244 |
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245 |
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246 - XXX.alleles.tsv file: |
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247 |
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248 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_ |
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249 |
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250 @STACKS_INFOS@ |
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251 ]]> |
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252 </help> |
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253 <expand macro="citation" /> |
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254 </tool> |