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| author | kpbioteam |
|---|---|
| date | Sun, 11 Feb 2018 07:35:45 -0500 |
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<tool id="minfi_ppquantile" name="minfi_ppquantile" version="0.1.0"> <description>implements stratified quantile normalization preprocessing</description> <requirements> <requirement type="package" version="1.24.0">bioconductor-minfi</requirement> <requirement type="package" version="0.4.0">bioconductor-illuminahumanmethylation450kmanifest</requirement> <requirement type="package" version="0.6.0">bioconductor-illuminahumanmethylation450kanno.ilmn12.hg19</requirement> </requirements> <command detect_errors="exit_code"><![CDATA[ Rscript ${__tool_directory__}/minfi_ppquantile.R "$input1" "$output1" ]]></command> <inputs> <param type="data" name="input1" format="rdata" /> </inputs> <outputs> <data name="output1" format="rdata" /> </outputs> <tests> <test> <param name="input1" value="RGSet.rdata"/> <output name="output1" file="quantile.rdata"/> </test> </tests> <help><![CDATA[ The normalization procedure is applied to the Meth and Unmeth intensities separately. The distribution of type I and type II signals is forced to be the same by first quantile normalizing the type II probes across samples and then interpolating a reference distribution to which we normalize the type I probes. Since probe types and probe regions are confounded and we know that DNAm distributions vary across regions we stratify the probes by region before applying this interpolation. ]]></help> <citations> <citation type="doi">10.1093/bioinformatics/btu049</citation> </citations> </tool>
