Mercurial > repos > iuc > mothur_filter_seqs

annotate filter.seqs.xml @ 3:3ced7f00e40b draft

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
author iuc
date Wed, 14 Feb 2018 09:40:41 -0500
parents 74e541cb0f87
children ff039df26c3f
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
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iuc
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1 <tool profile="16.07" id="mothur_filter_seqs" name="Filter.seqs" version="@WRAPPER_VERSION@.0">
32f444a36d12 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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2 <description>removes columns from alignments</description>
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3 <macros>
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4 <import>macros.xml</import>
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iuc
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5 </macros>
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6 <expand macro="requirements"/>
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7 <expand macro="stdio"/>
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8 <expand macro="version_command"/>
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9 <command><![CDATA[
3
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10 @SHELL_OPTIONS@
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11
3
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12 ## create symlinks to input datasets
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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13 ln -s '$fasta' fasta.dat &&
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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14 #for $i in $inputs:
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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15 ln -s '$i.fasta' fasta${inputs.index($i)}.dat &&
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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16 #end for
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17 ln -s '$hard' hard.dat &&
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18
3
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19 echo 'filter.seqs(
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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20 fasta=fasta.dat#for $i in $inputs#-fasta${inputs.index($i)}.dat#end for#,
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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21 vertical=$vertical,
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22 #if $trump:
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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23 trump=$trump,
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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24 #end if
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25 soft=$soft,
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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26 #if $hard:
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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27 hard=hard.dat,
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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28 #end if
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29 processors='\${GALAXY_SLOTS:-8}'
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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30 )'
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31 | sed 's/ //g' ## mothur trips over whitespace
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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32 | mothur
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33 | tee mothur.out.log &&
0
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34
3
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35 ## rename collection files for more transparent element naming
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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36 mv fasta.filter.fasta "${fasta.element_identifier}".filter.fasta
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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37 #for i in $inputs:
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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38 && mv fasta${inputs.index($i)}.filter.fasta "${i.fasta.element_identifier}".filter.fasta
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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39 #end for
0
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40 ]]></command>
32f444a36d12 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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41 <inputs>
32f444a36d12 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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42 <param name="fasta" type="data" format="mothur.align" label="fasta - Alignment Fasta"/>
1
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43 <repeat name="inputs" title="Additional Alignment File">
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44 <param name="fasta" type="data" format="mothur.align" label="fasta - Alignment Fasta"/>
32f444a36d12 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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45 </repeat>
3
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46 <param name="vertical" type="boolean" checked="true" truevalue="true" falsevalue="false" label="vertical - Vertical column"
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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47 help="Ignore any column that only contains gap characters (i.e. '-' or '.')"/>
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48 <param name="trump" type="select" label="trump - Trump character"
3ced7f00e40b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
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49 help="Remove a column if the trump character is found at that position in any sequence of the alignment">
0
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50 <option value="">Off</option>
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51 <option value=".">.</option>
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52 <option value="-">-</option>
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53 <option value="N">N</option>
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54 </param>
3
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55 <param name="soft" type="integer" value="0" min="0" max="100" label="soft - percentage required to retain column. (0-100)"
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56 help="Removes any column where the dominant base (i.e. A, T, G, C, or U) does not occur in at least a designated percentage of sequences"/>
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57 <param name="hard" type="data" format="mothur.filter" optional="True" label="hard - Hard Column Filter"
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58 help="A file should only contain one line consisting of 0's and 1's"/>
0
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59 </inputs>
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60 <outputs>
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61 <expand macro="logfile-output"/>
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62 <data name="out_filter" format="mothur.filter" from_work_dir="fasta*.filter" label="${tool.name} on ${on_string}: filter"/>
32f444a36d12 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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63 <collection name="filteredfastas" type="list" label="${tool.name} on ${on_string}: filtered fastas">
32f444a36d12 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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64 <discover_datasets pattern="(?P&lt;designation&gt;.*)\.filter\.fasta" format="fasta"/>
1
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65 <filter>inputs</filter> <!-- only output collection if multiple outputs-->
0
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66 </collection>
1
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67 <data name="filteredfasta" format="fasta" from_work_dir="*.filter.fasta" label="${tool.name} on ${on_string}: filtered fasta">
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68 <filter>not inputs</filter>
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69 </data>
0
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70 </outputs>
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71 <tests>
1
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72 <test><!-- test with multiple inputs and collection output -->
0
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73 <param name="fasta" value="HMP_MOCK.v35.align" ftype="mothur.align"/>
32f444a36d12 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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74 <repeat name="inputs">
32f444a36d12 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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75 <param name="fasta" value="Mock_S280_L001_R1_001_small.trim.contigs.good.align_head" ftype="mothur.align"/>
32f444a36d12 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 180a403421967d36f995941b1a4561349d75cfc5
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76 </repeat>
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77 <output name="out_filter" md5="3e6c2cfef46baf35d2a8b5cafe53e3a4"/>
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78 <output_collection name="filteredfastas" count="2">
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79 <element name="HMP_MOCK.v35.align" md5="ef4c6c2d9a882f7a22e5fa3c814af7cc" ftype="fasta"/>
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80 </output_collection>
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81 <expand macro="logfile-test"/>
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82 </test>
1
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83 <test><!-- test with single input and non-collection output -->
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84 <param name="fasta" value="HMP_MOCK.v35.align" ftype="mothur.align"/>
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85 <output name="out_filter" md5="3e6c2cfef46baf35d2a8b5cafe53e3a4"/>
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86 <output name="filteredfasta" md5="ef4c6c2d9a882f7a22e5fa3c814af7cc"/>
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87 <expand macro="logfile-test"/>
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88 </test>
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89 </tests>
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90 <help><![CDATA[
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91
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92 @MOTHUR_OVERVIEW@
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93
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94 **Command Documentation**
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95
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96 The filter.seqs_ command removes columns from alignments based on a criteria defined by the user. For example, alignments
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97 generated against reference alignments (e.g. from RDP, SILVA, or greengenes) often have columns where every character is
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98 either a '.' or a '-'. These columns are not included in calculating distances because they have no information in them.
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99 By removing these columns, the calculation of a large number of distances is accelerated. Also, people also like to mask
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100 their sequences to remove variable regions using a soft or hard mask (e.g. Lane's mask). This type of masking is only
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101 encouraged for deep-level phylogenetic analysis, not fine level analysis such as that needed with calculating OTUs.
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102
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103 .. _filter.seqs: https://www.mothur.org/wiki/Filter.seqs
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104
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105 v.1.20.0: Updated to Mothur 1.33
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106
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107 ]]></help>
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108 <expand macro="citations"/>
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109 </tool>