Mercurial > repos > iuc > mothur_filter_seqs
diff filter.seqs.xml @ 3:3ced7f00e40b draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 4648c7574a78601e03ae6a318cbcd5b492a8a9f4
author | iuc |
---|---|
date | Wed, 14 Feb 2018 09:40:41 -0500 |
parents | 74e541cb0f87 |
children | ff039df26c3f |
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--- a/filter.seqs.xml Tue Sep 05 16:21:39 2017 -0400 +++ b/filter.seqs.xml Wed Feb 14 09:40:41 2018 -0500 @@ -7,51 +7,55 @@ <expand macro="stdio"/> <expand macro="version_command"/> <command><![CDATA[ - @SHELL_OPTIONS@ +@SHELL_OPTIONS@ - ## create symlinks to input datasets - ln -s "$fasta" fasta.dat && - #for $i in $inputs: - ln -s "$i.fasta" fasta${inputs.index($i)}.dat && - #end for - ln -s "$hard" hard.dat && +## create symlinks to input datasets +ln -s '$fasta' fasta.dat && +#for $i in $inputs: + ln -s '$i.fasta' fasta${inputs.index($i)}.dat && +#end for +ln -s '$hard' hard.dat && - echo 'filter.seqs( - fasta=fasta.dat#for $i in $inputs#-fasta${inputs.index($i)}.dat#end for#, - vertical=$vertical, - #if $trump: - trump=$trump, - #end if - soft=$soft, - #if $hard: - hard=hard.dat, - #end if - processors='\${GALAXY_SLOTS:-8}' - )' - | sed 's/ //g' ## mothur trips over whitespace - | mothur - | tee mothur.out.log && +echo 'filter.seqs( + fasta=fasta.dat#for $i in $inputs#-fasta${inputs.index($i)}.dat#end for#, + vertical=$vertical, + #if $trump: + trump=$trump, + #end if + soft=$soft, + #if $hard: + hard=hard.dat, + #end if + processors='\${GALAXY_SLOTS:-8}' +)' +| sed 's/ //g' ## mothur trips over whitespace +| mothur +| tee mothur.out.log && - ## rename collection files for more transparent element naming - mv fasta.filter.fasta "${fasta.element_identifier}".filter.fasta - #for i in $inputs: - && mv fasta${inputs.index($i)}.filter.fasta "${i.fasta.element_identifier}".filter.fasta - #end for +## rename collection files for more transparent element naming +mv fasta.filter.fasta "${fasta.element_identifier}".filter.fasta +#for i in $inputs: + && mv fasta${inputs.index($i)}.filter.fasta "${i.fasta.element_identifier}".filter.fasta +#end for ]]></command> <inputs> <param name="fasta" type="data" format="mothur.align" label="fasta - Alignment Fasta"/> <repeat name="inputs" title="Additional Alignment File"> <param name="fasta" type="data" format="mothur.align" label="fasta - Alignment Fasta"/> </repeat> - <param name="vertical" type="boolean" checked="true" truevalue="true" falsevalue="false" label="vertical - Vertical column" help="Ignore any column that only contains gap characters (i.e. '-' or '.')"/> - <param name="trump" type="select" label="trump - Trump character" help="Remove a column if the trump character is found at that position in any sequence of the alignment"> + <param name="vertical" type="boolean" checked="true" truevalue="true" falsevalue="false" label="vertical - Vertical column" + help="Ignore any column that only contains gap characters (i.e. '-' or '.')"/> + <param name="trump" type="select" label="trump - Trump character" + help="Remove a column if the trump character is found at that position in any sequence of the alignment"> <option value="">Off</option> <option value=".">.</option> <option value="-">-</option> <option value="N">N</option> </param> - <param name="soft" type="integer" value="0" min="0" max="100" label="soft - percentage required to retain column. (0-100)" help="Removes any column where the dominant base (i.e. A, T, G, C, or U) does not occur in at least a designated percentage of sequences"/> - <param name="hard" type="data" format="mothur.filter" optional="True" label="hard - Hard Column Filter" help="A file should only contain one line consisting of 0's and 1's"/> + <param name="soft" type="integer" value="0" min="0" max="100" label="soft - percentage required to retain column. (0-100)" + help="Removes any column where the dominant base (i.e. A, T, G, C, or U) does not occur in at least a designated percentage of sequences"/> + <param name="hard" type="data" format="mothur.filter" optional="True" label="hard - Hard Column Filter" + help="A file should only contain one line consisting of 0's and 1's"/> </inputs> <outputs> <expand macro="logfile-output"/> @@ -83,20 +87,23 @@ <expand macro="logfile-test"/> </test> </tests> - <help> -<![CDATA[ + <help><![CDATA[ @MOTHUR_OVERVIEW@ **Command Documentation** -The filter.seqs_ command removes columns from alignments based on a criteria defined by the user. For example, alignments generated against reference alignments (e.g. from RDP, SILVA, or greengenes) often have columns where every character is either a '.' or a '-'. These columns are not included in calculating distances because they have no information in them. By removing these columns, the calculation of a large number of distances is accelerated. Also, people also like to mask their sequences to remove variable regions using a soft or hard mask (e.g. Lane's mask). This type of masking is only encouraged for deep-level phylogenetic analysis, not fine level analysis such as that needed with calculating OTUs. +The filter.seqs_ command removes columns from alignments based on a criteria defined by the user. For example, alignments +generated against reference alignments (e.g. from RDP, SILVA, or greengenes) often have columns where every character is +either a '.' or a '-'. These columns are not included in calculating distances because they have no information in them. +By removing these columns, the calculation of a large number of distances is accelerated. Also, people also like to mask +their sequences to remove variable regions using a soft or hard mask (e.g. Lane's mask). This type of masking is only +encouraged for deep-level phylogenetic analysis, not fine level analysis such as that needed with calculating OTUs. .. _filter.seqs: https://www.mothur.org/wiki/Filter.seqs v.1.20.0: Updated to Mothur 1.33 -]]> - </help> + ]]></help> <expand macro="citations"/> </tool>