Mercurial > repos > eugen > bs_seq_test_2
changeset 10:4d37a7026737 draft default tip
Uploaded
| author | eugen |
|---|---|
| date | Thu, 16 Aug 2012 04:58:24 -0400 |
| parents | 1d716ddd7511 |
| children | |
| files | methratio.patch |
| diffstat | 1 files changed, 50 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/methratio.patch Thu Aug 16 04:58:24 2012 -0400 @@ -0,0 +1,50 @@ +--- methratio.py.orig 2012-04-10 20:54:39.000000000 +0200 ++++ methratio.py 2012-08-16 10:54:42.000000000 +0200 +@@ -1,4 +1,5 @@ +-import sys, time, os, array, optparse ++#!/usr/bin/python ++import sys, time, os, array, optparse, re + usage = "usage: %prog [options] BSMAP_MAPPING_FILES" + parser = optparse.OptionParser(usage=usage) + +@@ -128,26 +129,32 @@ + + disp('writing %s ...' % options.outfile) + ss = {'C': '+', 'G': '-'} ++trint = {'CG[ACGT]$':'CPG','C[ACT]G$':'CHG', r"C[ACT][ACT]":'CHH', r"[ACGT]CG":'CPG',r"C[AGT]G":'CHG',r"[AGT][AGT]G":'CHH' } + fout = open(options.outfile, 'w') +-z95, z95sq = 1.96, 1.96 * 1.96 +-fout.write('chr\tpos\tstrand\tcontext\tratio\ttotal_C\tmethy_C\tCI_lower\tCI_upper\n') ++ ++fout.write('chr\tpos\tstrand\tcontext\coverage\tmethylated_C\tperc_methy_C\n') + nc, nd, dep0 = 0, 0, options.min_depth + for cr in sorted(depth.keys()): + depthcr, methcr, refcr = depth[cr], meth[cr], ref[cr] + for i, d in enumerate(depthcr): ++ if i < 2: continue + if d < dep0: continue + nc += 1 + nd += d + m = methcr[i] + if m == 0 and not options.meth0: continue + ratio = float(m) / d +- seq = refcr[i-2:i+3] + strand = ss[refcr[i]] +- pmid = ratio + z95sq / (2 * d) +- sd = z95 * ((ratio*(1-ratio)/d + z95sq/(4*d*d)) ** 0.5) +- norminator = 1 + z95sq / d +- CIl, CIu = (pmid - sd) / norminator, (pmid + sd) / norminator +- fout.write('%s\t%d\t%c\t%s\t%.3f\t%d\t%d\t%.3f\t%.3f\n' % (cr, i+1, strand, seq, ratio, d, m, CIl, CIu)) ++ if strand == '-': ++ seq = refcr[i-2:i+1] ++ else: ++ seq = refcr[i:i+3] ++ ++ for k, v in trint.items(): ++ if re.match(k,seq): ++ context=v ++ ++ fout.write('%s\t%d\t%c\t%s\t%d\t%d\t%.2f\n' % (cr, i+1, strand, context, d, m, m*100/d)) + + fout.close() + disp('done.')