Mercurial > repos > devteam > weblogo3

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/rgWebLogo3.py	Thu Jan 23 12:30:59 2014 -0500
@@ -0,0 +1,157 @@
+"""
+# modified june 2 ross lazarus to add units option at Assaf Gordon's suggestion
+# rgWebLogo3.py
+# wrapper to check that all fasta files are same length
+
+"""
+import optparse, os, sys, subprocess, tempfile
+
+WEBLOGO = 'weblogo' # executable name for weblogo3 - confusing isn't it?
+
+class WL3:
+    """
+    simple wrapper class to check fasta sequence lengths are all identical
+    """
+    FASTASTARTSYM = '>'
+    badseq = '## error - sequences in file %s are not all the same length - cannot proceed. Please read the tool documentation carefully'
+
+    def __init__(self,opts=None):
+        assert opts<>None,'WL3 class needs opts passed in - got None'
+        self.opts = opts
+        self.fastaf = file(self.opts.input,'r')
+        self.clparams = {}
+
+    def whereis(self,program):
+        for path in os.environ.get('PATH', '').split(':'):
+            if os.path.exists(os.path.join(path, program)) and not os.path.isdir(os.path.join(path, program)):
+                return os.path.join(path, program)
+        return None
+
+    def runCL(self):
+        """ construct and run a command line
+        """
+        wl = self.whereis(WEBLOGO)
+        if not wl:
+            print >> sys.stderr, '## rgWebLogo3.py error - cannot locate the weblogo binary %s on the current path' % WEBLOGO
+            print >> sys.stderr, '## Please ensure it is installed and working from http://code.google.com/p/weblogo'
+            sys.exit(1)
+        cll = [WEBLOGO,]
+        cll += [' '.join(it) for it in list(self.clparams.items())]
+        cl = ' '.join(cll)
+        assert cl > '', 'runCL needs a command line as clparms'
+        fd,templog = tempfile.mkstemp(suffix='rgtempRun.txt')
+        tlf = open(templog,'w')
+        process = subprocess.Popen(cl, shell=True, stderr=tlf, stdout=tlf)
+        rval = process.wait()
+        tlf.close()
+        tlogs = ''.join(open(templog,'r').readlines())
+        if len(tlogs) > 1:
+            s = '## executing %s returned status %d and log (stdout/stderr) records: \n%s\n' % (cl,rval,tlogs)
+        else:
+            s = '## executing %s returned status %d. Nothing appeared on stderr/stdout\n' % (cl,rval)
+        os.unlink(templog) # always
+        if rval <> 0:
+            print >> sys.stderr, '## rgWebLogo3.py error - executing %s returned error code %d' % (cl,rval)
+            print >> sys.stderr, '## This may be a data problem or a tool dependency (%s) installation problem' % WEBLOGO
+            print >> sys.stderr, '## Please ensure %s is correctly installed and working on the command line -see http://code.google.com/p/weblogo' % WEBLOGO
+            sys.exit(1)
+        return s
+
+
+    def iter_fasta(self):
+        """
+        generator for fasta sequences from a file
+        """
+        aseq = []
+        seqname = None
+        for i,row in enumerate(self.fastaf):
+            if row.startswith(self.FASTASTARTSYM):
+                if seqname <> None: # already in a sequence
+                    s = ''.join(aseq)
+                    l = len(s)
+                    yield (seqname,l)
+                    seqname = row[1:].strip()
+                    aseq = []
+                else:
+                    if i > 0:
+                        print >> sys.stderr,'Invalid fasta file %s - does not start with %s - please read the tool documentation carefully' % (self.opts.input,self.FASTASTARTSYM)
+                        sys.exit(1)
+                    else:
+                        seqname = row[1:].strip()
+            else: # sequence row
+                if seqname == None:
+                    print >> sys.stderr,'Invalid fasta file %s - does not start with %s - please read the tool documentation carefully' % (self.opts.input,self.FASTASTARTSYM)
+                    sys.exit(1)
+                else:
+                    aseq.append(row.strip())
+
+        if seqname <> None: # last one
+            l = len(''.join(aseq))
+            yield (seqname,l)
+
+
+    def fcheck(self):
+        """ are all fasta sequence same length?
+        might be mongo big
+        """
+        flen = None
+        lasti = None
+        f = self.iter_fasta()
+        for i,(seqname,seqlen) in enumerate(f):
+            lasti = i
+            if i == 0:
+                flen = seqlen
+            else:
+                if seqlen <> flen:
+                    print >> sys.stderr,self.badseq % self.opts.input
+                    sys.exit(1)
+        return '# weblogo input %s has %d sequences all of length %d' % (self.opts.input,lasti,flen)
+
+
+    def run(self):
+        check = self.fcheck()
+        self.clparams['-f'] = self.opts.input
+        self.clparams['-o'] = self.opts.output
+        self.clparams['-t'] = '"%s"' % self.opts.logoname # must be wrapped as a string
+        self.clparams['-F'] = self.opts.outformat
+        if self.opts.size <> None:
+            self.clparams['-s'] = self.opts.size
+        if self.opts.lower <> None:
+            self.clparams['-l'] = self.opts.lower
+        if self.opts.upper <> None:
+            self.clparams['-u'] = self.opts.upper
+        if self.opts.colours <> None:
+            self.clparams['-c'] = self.opts.colours
+        if self.opts.units <> None:
+            self.clparams['-U'] = self.opts.units
+        s = self.runCL()
+        return check,s
+
+
+if __name__ == '__main__':
+    '''
+    called as
+<command interpreter="python">
+    rgWebLogo3.py --outformat $outformat -s $size -i $input -o $output -t "$logoname" -c "$colours"
+#if $range.mode == 'part'
+-l "$range.seqstart" -u "$range.seqend"
+#end if
+    </command>
+
+    '''
+    op = optparse.OptionParser()
+    op.add_option('-i', '--input', default=None)
+    op.add_option('-F', '--outformat', default='png')
+    op.add_option('-s', '--size', default=None)
+    op.add_option('-o', '--output', default='rgWebLogo3')
+    op.add_option('-t', '--logoname', default='rgWebLogo3')
+    op.add_option('-c', '--colours', default=None)
+    op.add_option('-l', '--lower', default=None)
+    op.add_option('-u', '--upper', default=None)
+    op.add_option('-U', '--units', default=None)
+    opts, args = op.parse_args()
+    assert opts.input <> None,'weblogo3 needs a -i parameter with a fasta input file - cannot open'
+    assert os.path.isfile(opts.input),'weblogo3 needs a valid fasta input file - cannot open %s' % opts.input
+    w = WL3(opts)
+    checks,s = w.run()
+    print >> sys.stdout, checks # for info
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/rgWebLogo3.xml	Thu Jan 23 12:30:59 2014 -0500
@@ -0,0 +1,141 @@
+<tool id="rgweblogo3" name="Sequence Logo" version="0.4">
+   <description>generator for fasta (eg Clustal alignments)</description>
+   <requirements>
+      <requirement type="package" version="3.3">weblogo</requirement>
+      <requirement type="package" version="9.10">ghostscript</requirement>
+   </requirements>
+   <command interpreter="python">
+    rgWebLogo3.py -F $outformat -s $size -i $input -o $output -t "$logoname" -c "$colours" -U "$units"
+#if $range.mode == 'part'
+-l "$range.seqstart" -u "$range.seqend"
+#end if
+    </command>
+  <inputs>
+   <page>
+    <param format="fasta" name="input" type="data" label="Fasta File" />
+    <param name="logoname" label="Title for output Sequence Logo" type="text" size="50" value="Galaxy-Rgenetics Sequence Logo" />
+    <param name="outformat" type="select" label="Output format for image (or text report)" >
+      <option value="png" selected="True">PNG screen quality</option>
+      <option value="png_print">High quality printable PNG</option>
+      <option value="pdf">PDF</option>
+      <option value="jpeg">JPG</option>
+      <option value="eps">EPS</option>
+      <option value="txt">Text (shows the detailed calculations for each position - no image)</option>
+    </param>
+    <param name="units" type="select" label="Display Units"
+      help="What the height of each logo element depicts - eg bits of entropy (default)">
+      <option value="bits" selected="True">Entropy (bits)</option>
+      <option value="probability">Probability</option>
+      <option value="nats">Nats</option>
+      <option value="kT">kT</option>
+      <option value="kJ/mol">kJ/mol</option>
+      <option value="kcal/mol">kcal/mol</option>
+    </param>
+    <param name="colours" type="select" label="Colour scheme for output Sequence Logo"
+      help="Note that some of these only make sense for protein sequences!">
+      <option value="auto" selected="True">Default automatic colour selection</option>
+      <option value="base pairing">Base pairing</option>
+      <option value="charge">Charge colours</option>
+      <option value="chemistry">Chemistry colours</option>
+      <option value="classic">Classical colours</option>
+      <option value="hydrophobicity">Hydrophobicity</option>
+      <option value="monochrome">monochrome</option>
+    </param>
+
+
+    <conditional name="range">
+        <param name="mode" type="select" label="Include entire sequence (default) or specify a subsequence range to use">
+          <option value="complete" selected="true">complete sequence</option>
+          <option value="part">Only use a part of the sequence</option>
+        </param>
+        <when value="complete">
+        </when>
+        <when value="part">
+           <param name="seqstart" size="5" type="integer" value="1" help="WARNING: Specifying indexes outside the sequence lengths will cause unpredictable but bad consequences!"
+             label="Index (eg 1=first letter) of the start of the sequence range to include in the logo">
+           </param>
+           <param name="seqend" size="5" type="integer" value="99999" label="Index (eg 75=75th letter) of the end of the sequence range to include in the logo" >
+           </param>
+        </when>
+    </conditional>
+    <param name="size" type="select" label="Output weblogo size" >
+      <option value="large" selected="True">Large</option>
+      <option value="medium">Medium</option>
+      <option value="small">Small</option>
+    </param>
+   </page>
+  </inputs>
+  <outputs>
+    <data format="pdf" name="output"  label="${logoname}_output.${outformat}">
+       <change_format>
+           <when input="outformat" value="png_print" format="png" />
+           <when input="outformat" value="png" format="png" />
+           <when input="outformat" value="jpeg" format="jpg" />
+           <when input="outformat" value="eps" format="eps" />
+           <when input="outformat" value="txt" format="txt" />
+       </change_format>
+    </data>
+  </outputs>
+  <tests>
+    <test>
+      <param name="input" value="rgClustal_testout.fasta" />
+      <param name="logoname" value="Galaxy/Rgenetics weblogo" />
+      <param name="outformat" value="jpeg" />
+      <param name="mode" value="complete" />
+      <param name="size" value="medium" />
+      <param name="colours" value="auto" />
+      <param name="units" value="bits" />
+      <output name="output" file="rgWebLogo3_test.jpg" ftype="jpg" compare="sim_size" delta="10000" />
+    </test>
+    <test>
+      <param name="input" value="rgClustal_testout.fasta" />
+      <param name="logoname" value="Galaxy/Rgenetics weblogo" />
+      <param name="outformat" value="png" />
+      <param name="mode" value="complete" />
+      <param name="size" value="medium" />
+      <param name="colours" value="auto" />
+      <param name="units" value="probability" />
+      <output name="output" file="rgWebLogo3_test2.png" ftype="png" compare="sim_size" delta="10000" />
+    </test>
+  </tests>
+  <help>
+
+**Note**
+
+This tool uses Weblogo3_ in Galaxy to generate a sequence logo. The input file must be a fasta file in your current history.
+
+It is recommended for (eg) viewing multiple sequence alignments output from the clustalw tool - set the output to fasta and feed
+it in to this tool.
+
+A typical output looks like this
+
+.. image:: ${static_path}/images/rgWebLogo3_test.jpg
+
+----
+
+**Warning about input Fasta format files**
+
+The Weblogo3 program used by this tool will fail if your fasta sequences are not all EXACTLY the same length. The tool will provide a warning
+and refuse to call the weblogo3 executable if irregular length sequences are detected.
+
+Fasta alignments from the companion ClustalW Galaxy tool will work but many other fasta files may cause this tool to fail - please do not file
+a Galaxy bug report - this is a feature of the tool and a problem with your source data - not a tool error - please make certain all your fasta
+sequences are the same length!
+
+----
+
+**Attribution**
+
+Weblogo attribution and associated documentation are available at Weblogo3_
+
+This Galaxy wrapper was written by Ross Lazarus for the rgenetics project and the source code is licensed under the LGPL_ like other rgenetics artefacts
+
+.. _Weblogo3: http://weblogo.berkeley.edu/
+
+.. _LGPL: http://www.gnu.org/copyleft/lesser.html
+
+  </help>
+
+</tool>
+
+
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/rgClustal_testout.fasta	Thu Jan 23 12:30:59 2014 -0500
@@ -0,0 +1,48 @@
+>c_briggsae-chrII_+_
+---ATGAGCTTCCACAAAAGCATGAGCTTT
+CTCAGCTTCTGCCACATCAGCATTCAAATG
+ATC
+>c_brenneri-Cbre_Contig60_+_
+---ATGAGCCTCCACAACAGCATGATTTTT
+CTCGGCTTCCGCCACATCCGCATTCAAATG
+ATC
+>c_remanei-Crem_Contig172_-_
+---ATGAGCCTCTACAACCGCATGATTCTT
+TTCAGCCTCTGCCACGTCCGCATTCAAATG
+CTC
+>c_elegans-II_+_
+---ATGAGCCTCTACTACAGCATGATTCTT
+CTCAGCTTCTGCAACGTCAGCATTCAGATG
+ATC
+>c_briggsae-chrII_+_bar
+---CCGGAGTCGATCCCTGAAT--------
+------------------------------
+---
+>c_brenneri-Cbre_Contig60fee_+_
+---ACGAAGTCGATCCCTGAAA--------
+-TCAGATGAGCGGTTGACCA---GAGAACA
+ACC
+>c_remanei-Crem_Contig172zot_-_
+---ACGAAGTCGGTCCCTATAAGGTATGAT
+TTTATATGA----TGTACCATAAGGAAATA
+GTC
+>c_elegans-II_+_meh
+---ACGAAGTCGGTCCCTGAAC--AATTAT
+TT----TGA----TATA---GAAAGAAACG
+GTA
+>c_briggsae-chrIfooI_+_
+CGCACAAATATGATGCACAAATCCACAACC
+TAAAGCATCTCCGATAACGTTGACCGAAGT
+---
+>c_brenneri-Cbre_Contig60gak_+_
+CGCACAAATGTAGTGGACAAATCCGCATCC
+CAAAGCGTCTCCGATAACATTTACCGAAGT
+---
+>c_remanei-Crem_Contig172foo_-_
+AGCACAAATGTAATGAACGAATCCGCATCC
+CAACGCATCGCCAATCACATTCACAGATGT
+---
+>c_elegans-II_+_more
+TGCACAAATGTGATGAACGAATCCACATCC
+CAATGCATCACCGATCACATTGACAGATGT
+---
Binary file test-data/rgWebLogo3_test.jpg has changed
Binary file test-data/rgWebLogo3_test2.png has changed
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_dependencies.xml	Thu Jan 23 12:30:59 2014 -0500
@@ -0,0 +1,9 @@
+<?xml version="1.0"?>
+<tool_dependency>
+  <package name="ghostscript" version="9.10">
+      <repository changeset_revision="9345d2740f0c" name="package_ghostscript_9_10" owner="devteam" prior_installation_required="False" toolshed="http://testtoolshed.g2.bx.psu.edu" />
+    </package>
+    <package name="weblogo" version="3.3">
+      <repository changeset_revision="18cb94906560" name="package_weblogo_3_3" owner="devteam" prior_installation_required="False" toolshed="http://testtoolshed.g2.bx.psu.edu" />
+    </package>
+</tool_dependency>