changeset 5:d2d9937d1f9c draft

Uploaded
author chrisw
date Tue, 12 Feb 2019 13:51:52 -0500
parents ae546ad6f2f4
children 4104eb494bf8
files .shed.yml monorail.sh monorail.xml tool_dependencies.xml
diffstat 4 files changed, 18 insertions(+), 1 deletions(-) [+]
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/.shed.yml	Tue Feb 12 13:51:52 2019 -0500
@@ -0,0 +1,10 @@
+categories:
+    - RNA
+description: Monorail is a RNA-seq analysis which produces coverage & junction summaries
+long_description: |
+    Monorail is a RNA-seq analysis pipeline which produces summarized coverage and junction information w/o the cost of keeping BAMs.
+    It runs STAR, samtools, and bamcount at its core to produce its results efficiently.
+name: monorail
+owner: ChristopherWilks
+remote_repository_url: https://github.com/ChristopherWilks/monorail_galaxy
+type: unrestricted
--- a/monorail.sh	Tue Feb 12 11:56:25 2019 -0500
+++ b/monorail.sh	Tue Feb 12 13:51:52 2019 -0500
@@ -29,4 +29,4 @@
 samtools index -@ $threads $sorted_bam >> stools.log 2>&1
 
 #get coverage summaries
-bamcount $sorted_bam --threads $threads --coverage --no-head --require-mdz --min-unique-qual $min_uniq_qual --frag-dist bc --bigwig bc --annotation $annotation bc --auc bc --lats bc > bc.log 2>&1 
+bamcount $sorted_bam --threads $threads --coverage --no-head --require-mdz --min-unique-qual $min_uniq_qual --frag-dist bc --bigwig bc --annotation $annotation bc --auc bc --alts bc > bc.log 2>&1 
--- a/monorail.xml	Tue Feb 12 11:56:25 2019 -0500
+++ b/monorail.xml	Tue Feb 12 13:51:52 2019 -0500
@@ -2,6 +2,7 @@
     <requirements>
         <requirement type="package" version="1.9">samtools</requirement>
         <requirement type="package" version="2.6.0b">star</requirement>
+        <requirement type="package" version="0.2.6">bamcount</requirement>
         <!-- <requirement type="package" version="5.4.0">snakemake-minimal</requirement> -->
     </requirements>
         <!-- /bin/bash -x monorail.slim.sh ../ath10 4 10 ../ath10/gtf/exons.bed ./tmp2 ../fastqs/SRR8505407_1_100.fastq.gz ../fastqs/SRR8505407_2_100.fastq.gz -->
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_dependencies.xml	Tue Feb 12 13:51:52 2019 -0500
@@ -0,0 +1,6 @@
+<?xml version="1.0"?>
+<tool_dependency>
+    <package name="bamcount" version="0.2.6">
+        <repository changeset_revision="f02b4483eda2" name="bamcount_test" owner="chrisw" prior_installation_required="False" toolshed="http://testtoolshed.g2.bx.psu.edu" />
+    </package>
+</tool_dependency>