annotate salmon.xml @ 11:c320b539ff54 draft

planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
author bgruening
date Thu, 25 Jul 2019 15:45:02 -0400
parents d9bfffca4548
children
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
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83bbe4e20bdc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 1b7f0370c3a5a8fd4ba2736607ad938a82a98b7b
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1 <tool id="salmon" name="Salmon" version="@VERSION@">
0
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2
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3 <description>Transcript Quantification from RNA-seq data</description>
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4
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5 <macros>
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6 <xml name="strandedness">
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7 <param name="strandedness" type="select" label="Specify the strandedness of the reads">
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8 <option value="U" selected="True">Not stranded (U)</option>
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9 <option value="SF">read 1 (or single-end read) comes from the forward strand (SF)</option>
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10 <option value="SR">read 1 (or single-end read) comes from the reverse strand (SR)</option>
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11 </param>
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12 </xml>
8
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13 <xml name="orientation">
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14 <param name="orientation" type="select" label="Relative orientation of reads within a pair">
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15 <option value="M">Mates are oriented in the same direction (M = matching)</option>
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16 <option value="O">Mates are oriented away from each other (O = outward)</option>
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17 <option value="I" selected="True">Mates are oriented toward each other (I = inward)</option>
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18 </param>
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19 </xml>
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20 <token name="@VERSION@">0.11.2</token>
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21 <token name="@IDX_VERSION@">q5</token>
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22 </macros>
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23
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24 <requirements>
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25 <requirement type="package" version="1.0.6">bzip2</requirement>
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26 <requirement type="package" version="@VERSION@">salmon</requirement>
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27 <requirement type="package" version="1.2">seqtk</requirement>
0
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28 </requirements>
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29
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30 <stdio>
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31 <exit_code range="1:" />
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32 <exit_code range=":-1" />
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33 <regex match="Error:" />
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34 <regex match="Exception:" />
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35 <regex match="Exception :" />
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36 </stdio>
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37 <version_command>salmon -version</version_command>
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38 <command><![CDATA[
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39 mkdir ./index
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40 &&
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41 mkdir ./output
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42 #if $refTranscriptSource.TranscriptSource == "history":
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43 &&
0
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44 salmon index
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45 --transcripts $refTranscriptSource.ownFile
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46 --kmerLen $refTranscriptSource.kmerLen
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47 --threads "\${GALAXY_SLOTS:-4}"
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48 --index './index'
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49 --type '$quasi_orphans.type'
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50 $perfectHash
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51 #set $index_path = './index'
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52 #else:
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53 #set $index_path = $refTranscriptSource.index.fields.path
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54 #end if
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55 &&
4
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56 #set compressed = 'no'
0
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57 #if $single_or_paired.single_or_paired_opts == 'single':
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58 #if $single_or_paired.input_singles.ext == 'fasta':
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59 #set $ext = 'fasta'
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60 #else:
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61 #if $single_or_paired.input_singles.is_of_type("fastq.gz", "fastqsanger.gz"):
4
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62 #set compressed = 'GZ'
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63 #else if $single_or_paired.input_singles.is_of_type("fastq.bz2", "fastqsanger.bz2"):
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64 #set compressed = 'BZ2'
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65 #end if
0
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66 #set $ext = 'fastq'
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67 #end if
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68 ln -s $single_or_paired.input_singles ./single.$ext &&
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69 #else if $single_or_paired.single_or_paired_opts == 'paired':
0
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70 #if $single_or_paired.input_mate1.ext == 'fasta':
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71 #set $ext = 'fasta'
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72 #else:
8
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73 #if $single_or_paired.input_mate1.is_of_type("fastq.gz", "fastqsanger.gz"):
4
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74 #set compressed = 'GZ'
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75 #else if $single_or_paired.input_mate1.is_of_type("fastq.bz2", "fastqsanger.bz2"):
4
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76 #set compressed = 'BZ2'
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77 #end if
0
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78 #set $ext = 'fastq'
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79 #end if
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80 ln -s $single_or_paired.input_mate1 ./mate1.$ext &&
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81 ln -s $single_or_paired.input_mate2 ./mate2.$ext &&
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82 #else if $single_or_paired.single_or_paired_opts == 'paired_collection':
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83 #if $single_or_paired.input_1.forward.ext == 'fasta':
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84 #set $ext = 'fasta'
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85 #else:
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86 #if $single_or_paired.input_1.forward.is_of_type("fastq.gz", "fastqsanger.gz"):
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87 #set compressed = 'GZ'
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88 #else if $single_or_paired.input_1.forward.is_of_type("fastq.bz2", "fastqsanger.bz2"):
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89 #set compressed = 'BZ2'
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90 #end if
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91 #set $ext = 'fastq'
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92 #end if
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93 ln -s ${single_or_paired.input_1.forward} ./mate1.$ext &&
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94 ln -s ${single_or_paired.input_1.reverse} ./mate2.$ext &&
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95 #else if $single_or_paired.single_or_paired_opts == 'paired_interleaved':
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96 #if $single_or_paired.input_1.ext == 'fasta':
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97 #set $ext = 'fasta'
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98 #else:
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99 #if $single_or_paired.input_1.is_of_type("fastq.gz", "fastqsanger.gz"):
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100 #set compressed = 'GZ'
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101 #else if $single_or_paired.input_1.is_of_type("fastq.bz2", "fastqsanger.bz2"):
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102 #set compressed = 'BZ2'
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103 #end if
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104 #set $ext = 'fastq'
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105 #end if
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106 ln -s $single_or_paired.input_1 ./mate1.$ext &&
0
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107 #end if
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108 #if $geneMap:
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109 ln -s "$geneMap" ./geneMap.${geneMap.ext} &&
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110 #end if
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diff changeset
111 salmon quant
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112 --index $index_path
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113 #if $single_or_paired.single_or_paired_opts == 'single':
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114 --libType ${single_or_paired.strandedness}
4
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115 #if $compressed == 'GZ':
8
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116 --unmatedReads <(zcat < ./single.$ext)
4
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117 #else if $compressed == 'BZ2':
8
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118 --unmatedReads <(bzcat < ./single.$ext)
4
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119 #else:
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120 --unmatedReads ./single.$ext
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121 #end if
0
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122 #else:
10
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123 --libType "${single_or_paired.orientation}${single_or_paired.strandedness}"
8
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124 #if $single_or_paired.single_or_paired_opts == 'paired_interleaved':
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125 #if $compressed == 'BZ2':
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126 --mates1 <(bzcat < ./mate1.$ext | seqtk seq -1)
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127 --mates2 <(bzcat < ./mate1.$ext | seqtk seq -2)
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128 #else:
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129 --mates1 <(seqtk seq -1 ./mate1.$ext)
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130 --mates2 <(seqtk seq -2 ./mate1.$ext)
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131 #end if
4
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132 #else:
8
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133 #if $compressed == 'GZ':
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134 --mates1 <(zcat < ./mate1.$ext)
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135 --mates2 <(zcat < ./mate2.$ext)
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136 #else if $compressed == 'BZ2':
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137 --mates1 <(bzcat < ./mate1.$ext)
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138 --mates2 <(bzcat < ./mate2.$ext)
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139 #else:
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140 --mates1 ./mate1.$ext
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141 --mates2 ./mate2.$ext
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142 #end if
4
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143 #end if
0
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144 #end if
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145 --output ./output
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146 #if str($quasi_orphans.type) == 'quasi':
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147 --allowOrphans
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148 $quasi_orphans.validateMappings
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149 --ma $quasi_orphans.matchScore
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150 --mp $quasi_orphans.mismatchPenalty
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151 --go $quasi_orphans.gapOpenPenalty
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152 --ge $quasi_orphans.gapExtensionPenalty
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153 --minScoreFraction $quasi_orphans.minScoreFraction
0
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154 #end if
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155 $seqBias
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156 $gcBias
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157 $noErrorModel
0
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158 --threads "\${GALAXY_SLOTS:-4}"
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159 --incompatPrior $adv.incompatPrior
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160 $adv.consistentHits
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161 $adv.dumpEq
8
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162 $adv.reduceGCMemory
0
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163 #if str($adv.biasSpeedSamp):
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164 --biasSpeedSamp $adv.biasSpeedSamp
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165 #end if
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166 $adv.strictIntersect
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167 #if str($adv.fldMax):
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168 --fldMax $adv.fldMax
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169 #end if
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170 #if str($adv.fldMean):
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171 --fldMean $adv.fldMean
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172 #end if
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173 #if str($adv.fldSD):
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174 --fldSD $adv.fldSD
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175 #end if
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176 #if $adv.forgettingFactor:
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177 --forgettingFactor $adv.forgettingFactor
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178 #end if
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179 $adv.initUniform
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180 $adv.noFragLengthDist
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181 $adv.noBiasLengthThreshold
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182 #if str($adv.maxReadOcc):
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183 --maxReadOcc $adv.maxReadOcc
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184 #end if
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185 #if $geneMap:
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186 --geneMap ./geneMap.${geneMap.ext}
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187 #end if
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188 $adv.noEffectiveLengthCorrection
10
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189 $adv.useEM
0
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190 #if str($adv.numBiasSamples):
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191 --numBiasSamples $adv.numBiasSamples
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192 #end if
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193 #if str($adv.numAuxModelSamples):
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194 --numAuxModelSamples $adv.numAuxModelSamples
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195 #end if
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196 #if str($adv.numPreAuxModelSamples):
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197 --numPreAuxModelSamples $adv.numPreAuxModelSamples
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198 #end if
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199 #if str($adv.numGibbsSamples):
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200 --numGibbsSamples $adv.numGibbsSamples
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201 #end if
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202 #if str($adv.numBootstraps):
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203 --numBootstraps $adv.numBootstraps
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204 #end if
10
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205 #if str($adv.consensusSlack):
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206 --consensusSlack $adv.consensusSlack
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207 #else:
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208 #if $quasi_orphans.validateMappings:
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209 --consensusSlack 1
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210 #else:
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211 --consensusSlack 0
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212 #end if
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213 #end if
0
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214 $adv.perTranscriptPrior
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215 #if $adv.vbPrior:
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216 --vbPrior $adv.vbPrior
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217 #end if
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218 $adv.writeUnmappedNames
10
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219 --sigDigits $adv.sigDigits
7
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220 #if str($adv.writeMappings):
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221 $adv.writeMappings > ${output_sam}
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222 #end if
0
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223 ]]>
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224 </command>
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225
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226 <inputs>
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227 <conditional name="refTranscriptSource">
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228 <param name="TranscriptSource" type="select" label="Select a reference transcriptome from your history or use a built-in index?" help="Built-ins were indexed using default options">
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229 <option value="indexed">Use a built-in index</option>
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230 <option value="history" selected="True">Use one from the history</option>
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231 </param>
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232 <when value="indexed">
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233 <param name="index" type="select" label="Select a reference transcriptome" help="If your transcriptome of interest is not listed, contact your Galaxy admin">
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234 <options from_data_table="salmon_indexes_versioned">
0
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235 <filter type="sort_by" column="2"/>
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236 <filter type="static_value" column="4" value="@IDX_VERSION@" />
0
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237 <validator type="no_options" message="No indexes are available for the selected input dataset"/>
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238 </options>
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239 </param>
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240 </when> <!-- build-in -->
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241 <when value="history">
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242 <param name="ownFile" type="data" format="fasta" label="Select the reference transcriptome" help="in FASTA format" />
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243 <param argument="kmerLen" type="integer" value="31" label="The size should be odd number."/>
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244 </when> <!-- history -->
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245 </conditional>
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246 <conditional name="single_or_paired">
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247 <param name="single_or_paired_opts" type="select" label="Is this library mate-paired?">
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248 <option value="single">Single-end</option>
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249 <option value="paired">Paired-end</option>
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250 <option value="paired_collection">Paired-end Dataset Collection</option>
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251 <option value="paired_interleaved">Paired-end data from single interleaved dataset</option>
0
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252 </param>
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253 <when value="single">
8
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254 <param name="input_singles" type="data" format="fastq,fasta,fastq.gz,fastq.bz2" label="FASTQ/FASTA file" help="FASTQ file." />
0
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255 <expand macro="strandedness" />
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256 </when>
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257 <when value="paired">
8
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258 <param name="input_mate1" type="data" format="fastq,fasta,fastq.gz,fastq.bz2,fastqsanger,fastqsanger.gz,fastqsanger.bz2" label="Mate pair 1" help="FASTQ file." />
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259 <param name="input_mate2" type="data" format="fastq,fasta,fastq.gz,fastq.bz2,fastqsanger,fastqsanger.gz,fastqsanger.bz2" label="Mate pair 2" help="FASTQ file." />
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260 <expand macro="orientation" />
0
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261 <expand macro="strandedness" />
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262 </when>
8
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263 <when value="paired_collection">
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264 <param name="input_1" format="fastq,fasta,fastq.gz,fastq.bz2,fastqsanger,fastqsanger.gz,fastqsanger.bz2" type="data_collection" collection_type="paired" label="FASTQ Paired Dataset" help="Must be of datatype &quot;fastqsanger&quot; or &quot;fasta&quot;" />
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265 <expand macro="orientation" />
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266 <expand macro="strandedness" />
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267 </when>
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268 <when value="paired_interleaved">
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269 <param name="input_1" format="fastq,fasta,fastq.gz,fastq.bz2,fastqsanger,fastqsanger.gz,fastqsanger.bz2" type="data" label="Interleaved FASTQ file" help="Must be of datatype &quot;fastqsanger&quot; or &quot;fasta&quot;. --interleaved"/>
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270 <expand macro="orientation" />
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271 <expand macro="strandedness" />
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272 </when>
0
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273 </conditional>
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274 <conditional name="quasi_orphans">
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275 <param argument="--type" type="select" label="Type of index" help="When using quasi, orphaned reads will be considered when performing lightweight-alignment.">
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276 <option value="quasi" selected="True">quasi</option>
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277 </param>
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278 <when value="quasi">
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279 <param argument="--validateMappings" type="boolean" truevalue="--validateMappings" falsevalue="" checked="False"
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280 label="Validate mappings"
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281 help="Validate mappings using alignment-based verifcation. If this flag is passed, quasi-mappings will be validated to ensure that they could give rise to a reasonable alignment before they are further used for quantification."/>
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282 <param name="matchScore" argument="--ma" type="integer" value="2"
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283 label="Match Score"
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284 help="The value given to a match between read and reference nucleotides in an alignment."/>
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285 <param name="mismatchPenalty" argument="--mp" type="integer" value="4"
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286 label="Mismatch Penalty"
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287 help="The value given to a mis-match between read and reference nucleotides in an alignment. This will be cast to a negative value."/>
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288 <param name="gapOpenPenalty" argument="--go" type="integer" value="5"
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289 label="Gap Open Penalty"
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290 help="The value given to a gap opening in an alignment."/>
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291 <param name="gapExtensionPenalty" argument="--ge" type="integer" value="3"
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292 label="Gap Extension Penalty"
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293 help="The value given to a gap extension in an alignment."/>
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294 <param argument="--minScoreFraction" type="float" value="0.65" min="0.0" max="0.99"
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295 label="Min Score Fraction"
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296 help="The fraction of the optimal possible alignment score that a mapping must achieve in order to be considered valid. Should be in (0,1]."/>
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297 </when> <!-- build-in -->
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298 </conditional>
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299 <param argument="--perfectHash" type="boolean" truevalue="--perfectHash" falsevalue="" checked="False"
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300 label="Perfect Hash"
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301 help="Build the index using a perfect hash rather than a dense hash. This will require less memory (especially during quantification), but will take longer to construct "/>
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302 <param argument="--seqBias" type="boolean" truevalue="--seqBias" falsevalue="" checked="False"
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303 label="Perform sequence-specific bias correction"
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304 help=""/>
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305 <param argument="--gcBias" type="boolean" truevalue="--gcBias" falsevalue="" checked="False"
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306 label="Perform fragment GC bias correction"
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307 help=""/>
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308 <param argument="--geneMap" type="data" format="tabular,gff,gtf" optional="True"
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309 label="File containing a mapping of transcripts to genes"
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310 help="If this file is provided Salmon will output both quant.sf and quant.genes.sf files, where the latter contains aggregated gene-level abundance estimates. The transcript to gene mapping should be provided as either a GTF file, or a in a simple tab-delimited format where each line contains the name of a transcript and the gene to which it belongs separated by a tab." />
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311 <param argument="--noErrorModel" type="boolean" truevalue="--noErrorModel" falsevalue="" checked="False"
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312 label="No Error Model"
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313 help="Turn off the alignment error model, which takes into account the the observed frequency of different types of mismatches / indels when computing the likelihood of a given alignment. Turning this off can speed up alignment-based salmon, but can harm quantification accuracy."/>
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314 <section name="adv" title="Additional Options">
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315 <param argument="--writeMappings" type="boolean" truevalue="--writeMappings" falsevalue="" checked="False"
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316 label="Write Mappings"
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317 help="If this option is set to 'Yes', then the quasi-mapping results will be written out in SAM-compatible format. By default, output is directed to stdout." />
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318 <param argument="--incompatPrior" type="float" optional="True" value="9.9999999999999995e-21"
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319 label="Incompatible Prior"
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320 help="This option sets the prior probability that an alignment that disagrees with the specified library type (--libType) results from the true fragment origin. Setting this to 0 specifies that alignments that disagree with the library type should be 'impossible', while setting it to 1 says that alignments that disagree with the library type are no less likely than those that do" />
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321 <param argument="--dumpEq" type="boolean" truevalue="--dumpEq" falsevalue="" checked="False"
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322 label="Dump the equivalence class counts that were computed during quasi-mapping." help=""/>
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323 <param argument="--reduceGCMemory" type="boolean" truevalue="--reduceGCMemory" falsevalue="" optional="True" checked="False"
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324 label="If this option is selected, a more memory efficient (but slightly slower representation is used to compute fragment GC content."
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325 help="Enabling this will reduce memory usage, but can also reduce speed. However, the results themselves will remain the same."/>
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326 <param argument="--biasSpeedSamp" type="integer" value="1" optional="True"
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327 label="The value at which the fragment length PMF is down-sampled when evaluating GC fragment bias." help="Larger values speed up effective length correction, but may decrease the fidelity of bias modeling results."/>
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328 <param argument="--strictIntersect" type="boolean" truevalue="--strictIntersect" falsevalue="" checked="False"
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329 label="Modifies how orphans are assigned." help="When this flag is set, if the intersection of the quasi-mappings for the left and right is empty, then all mappings for the left and all mappings for the right read are reported as orphaned quasi-mappings."/>
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330 <param argument="--minLen" type="integer" value="19" optional="True"
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331 label=" (S)MEMs smaller than this size won't be considered." help="" />
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332 <param argument="--sensitive" type="boolean" truevalue="--sensitive" falsevalue="" checked="False"
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333 label="Perform sensitive quantification"
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334 help=" Setting this option enables the splitting of SMEMs that are larger than 1.5 times the minimum seed length (minLen/k above). This may reveal high scoring chains of MEMs that are masked by long SMEMs. However, this option makes lightweight-alignment a bit slower and is usually not necessary if the reference is of reasonable quality." />
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335 <param argument="--consistentHits" type="boolean" truevalue="--consistentHits" falsevalue="" checked="False"
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336 label="Force hits gathered during quasi-mapping to be consistent"
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337 help="" />
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338 <param argument="--extraSensitive" type="boolean" truevalue="--extraSensitive" falsevalue="" checked="False"
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339 label="Perform extra sensitive quantification"
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340 help="Setting this option enables an extra pass of 'seed' search. Enabling this option may improve sensitivity (the number of reads having sufficient coverage), but will typically slow down quantification by ~40%. Consider enabling this option if you find the mapping rate to be significantly lower than expected."/>
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341 <param argument="--coverage" type="float" value="0.69999999999999996" optional="True"
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342 label="Required coverage of read by union of SMEMs to consider it a hit"
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343 help="" />
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344 <param argument="--fldMax" type="integer" value="1000" optional="True"
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345 label="The maximum fragment length to consider when building the empirical distribution."
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346 help=""/>
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347 <param argument="--fldMean" type="integer" value="200" optional="True"
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348 label="The mean used in the fragment length distribution prior"
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349 help="If single end reads are being used for quantification, or there are an insufficient number of uniquely mapping reads when performing paired-end quantification to estimate the empirical fragment length distribution, then use this value to calculate effective lengths."/>
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350 <param argument="--fldSD" type="integer" value="80" optional="True"
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351 label="Standard deviation"
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352 help="The standard deviation used in the fragment length distribution prior."/>
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353 <param argument="--forgettingFactor" type="float" value="0.65000000000000002" optional="True"
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354 label="The forgetting factor used in the online learning schedule."
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355 help=" A smaller value results in quicker learning, but higher variance and may be unstable. A larger value results in slower learning but may be more stable. Value should be in the interval (0.5, 1.0]." />
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356 <param argument="--initUniform" type="boolean" truevalue="--initUniform" falsevalue="" checked="False"
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357 label="Initialization with uniform parameters"
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358 help="initialize the offline inference with uniform parameters, rather than seeding with online parameters." />
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359 <param argument="--maxReadOcc" type="integer" value="100" optional="True"
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360 label="Maximal read mapping occurence"
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361 help="Reads mapping to more than this many places won't be considered."/>
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362 <param argument="--noEffectiveLengthCorrection" type="boolean" truevalue="--noEffectiveLengthCorrection" falsevalue="" checked="False"
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363 label="Disable effective length correction"
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364 help="Disables effective length correction when computing the probability that a fragment was generated from a transcript. If this flag is passed in, the fragment length distribution is not taken into account when computing this probability."/>
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365 <param argument="--noFragLengthDist" type="boolean" truevalue="--noFragLengthDist" falsevalue="" checked="False"
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366 label="Ignore fragment length distribution"
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367 help="[experimental] : Don't consider concordance with the learned fragment length distribution when trying to determine the probability that a fragment has originated from a specified location. Normally, Fragments with unlikely lengths will be assigned a smaller relative probability than those with more likely lengths. When this flag is passed in, the observed fragment length has no effect on that fragment's a priori probability." />
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368 <param argument="--noBiasLengthThreshold" type="boolean" truevalue="--noBiasLengthThreshold" falsevalue="" checked="False"
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369 label="[experimental] : If this option is enabled, then no (lower) threshold will be set on how short bias correction can make effecctive lengths."
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370 help="This can increase the precision of bias correction, but harm robustness. The default correction applies a threshold." />
0
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371 <param argument="--numBiasSamples" type="integer" value="2000000" optional="True"
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372 label="Number of fragment mappings to use when learning the sequence-specific bias model."
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373 help="" />
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374 <param argument="--numAuxModelSamples" type="integer" value="5000000" optional="True"
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375 label="The first numAuxModelSamples are used to train the auxiliary model parameters."
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376 help="(e.g. fragment length distribution, bias, etc.). After ther first numAuxModelSamples observations the auxiliary model parameters will be assumed to have converged and will be fixed." />
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377 <param argument="--numPreAuxModelSamples" type="integer" value="1000000" optional="True"
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378 label="The first numPreAuxModelSamples will have their assignment likelihoods and contributions to the transcript abundances computed without applying any auxiliary models."
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379 help=" The purpose of ignoring the auxiliary models for the first numPreAuxModelSamples observations is to avoid applying these models before thier parameters have been learned sufficiently well." />
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380 <param argument="--splitWidth" type="integer" value="0" optional="True"
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381 label=" If (S)MEM occurs fewer than this many times, search for smaller, contained MEMs"
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382 help="The default value will not split (S)MEMs, a higher value will result in more MEMs being explore and, thus, will result in increased running time." />
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383 <param argument="--splitSpanningSeeds" type="boolean" truevalue="--splitSpanningSeeds" falsevalue="" checked="False"
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384 label="Attempt to split seeds that happen to fall on the boundary between two transcripts."
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385 help="This can improve the fragment hit-rate, but is usually not necessary."/>
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386 <param argument="--useEM" type="boolean" truevalue="--useEM" falsevalue="" checked="False"
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387 label="Use the traditional EM algorithm for optimization in the batch passes."
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388 help=""/>
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389 <param argument="--numGibbsSamples" type="integer" value="0" optional="True"
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390 label=" Number of Gibbs sampling rounds to perform."
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391 help="" />
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392 <param argument="--numBootstraps" type="integer" value="0" optional="True"
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393 label="Number of bootstrap samples to generate. Note: This is mutually exclusive with Gibbs sampling."
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394 help="" />
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395 <param argument="--perTranscriptPrior" type="boolean" truevalue="--perTranscriptPrior" falsevalue="" checked="False"
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396 label="The prior will be interpreted as a transcript-level prior."
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397 help="either the default or the argument provided via --vbPrior" />
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398 <param argument="--vbPrior" type="float" value="0.001" optional="True"
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399 label="The prior that will be used in the VBEM algorithm."
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400 help="This is interpreted as a per-nucleotide prior, unless the --perTranscriptPrior flag is also given, in which case this is used as a transcript-level prior." />
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401 <param argument="--writeUnmappedNames" type="boolean" truevalue="--writeUnmappedNames" falsevalue="" checked="False"
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402 label="Write the names of un-mapped reads to the file unmapped_names.txt."
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403 help=""/>
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404 <param argument="--sigDigits" type="integer" value="3"
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405 label="Significant Digits"
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406 help="The number of significant digits to write when outputting the EffectiveLength and NumReads columns."/>
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407 <param argument="--consensusSlack" type="integer" optional="True"
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408 label="Consensus Slack"
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409 help="The amount of slack allowed in the quasi-mapping consensus mechanism. Normally, a transcript must cover all hits to be considered for mapping. If this is set to a value, X, greater than 0, then a transcript can fail to cover up to X hits before it is discounted as a mapping candidate. The default value of this option is 1 if --validateMappings is given and 0 otherwise."/>
0
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410 </section>
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411 </inputs>
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412
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413 <outputs>
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414 <data name="output_quant" format="tabular" from_work_dir="output/quant.sf" label="${tool.name} on ${on_string} (Quantification)" />
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415 <data name="output_gene_quant" format="tabular" from_work_dir="output/quant.genes.sf" label="${tool.name} on ${on_string} (Gene Quantification)">
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416 <filter>geneMap</filter>
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417 </data>
7
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418 <data name="output_sam" format="sam" label="${tool.name} on ${on_string} (SAM format)">
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419 <filter>adv['writeMappings']</filter>
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420 </data>
0
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421 </outputs>
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422
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423 <tests>
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424 <test>
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425 <param name="single_or_paired_opts" value="paired" />
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426 <param name="input_mate1" value="reads_1.fastq" />
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427 <param name="input_mate2" value="reads_2.fastq" />
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428 <param name="biasCorrect" value="False" />
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429 <param name="TranscriptSource" value="history" />
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430 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
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431 <output name="output_quant">
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diff changeset
432 <assert_contents>
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
433 <has_text text="EffectiveLength" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
434 <has_text text="TPM" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
435 <has_text text="NM_001168316" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
436 <has_text text="NM_174914" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
437 <has_text text="NM_018953" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
438 <has_text text="NR_003084" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
439 <has_text text="NM_017410" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
440 <has_text text="NM_153693" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
441 <has_text text="NR_031764" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
442 <has_n_columns n="5" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
443 </assert_contents>
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
444 </output>
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
445 </test>
11
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
446 <test> <!--test use of built-in index-->
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
447 <param name="single_or_paired_opts" value="paired" />
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
448 <param name="input_mate1" value="reads_1.fastq" />
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
449 <param name="input_mate2" value="reads_2.fastq" />
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
450 <param name="biasCorrect" value="False" />
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
451 <param name="TranscriptSource" value="indexed" />
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
452 <param name="index" value="hg19_transcript_subset" />
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
453 <output name="output_quant">
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
454 <assert_contents>
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
455 <has_text text="EffectiveLength" />
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
456 <has_text text="TPM" />
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
457 <has_text text="NM_001168316" />
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
458 <has_text text="NM_174914" />
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
459 <has_text text="NM_018953" />
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
460 <has_text text="NR_003084" />
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
461 <has_text text="NM_017410" />
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
462 <has_text text="NM_153693" />
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
463 <has_text text="NR_031764" />
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
464 <has_n_columns n="5" />
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
465 </assert_contents>
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
466 </output>
c320b539ff54 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 4cc21e8c47d99cee4560bb7634c859b551c4ea72
bgruening
parents: 10
diff changeset
467 </test>
4
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
468 <test> <!-- gzipped input -->
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
469 <param name="single_or_paired_opts" value="paired" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
470 <param name="input_mate1" value="reads_1.fastq.gz" ftype="fastqsanger.gz" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
471 <param name="input_mate2" value="reads_2.fastq.gz" ftype="fastqsanger.gz" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
472 <param name="biasCorrect" value="False" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
473 <param name="TranscriptSource" value="history" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
474 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
475 <output name="output_quant">
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
476 <assert_contents>
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
477 <has_text text="EffectiveLength" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
478 <has_text text="TPM" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
479 <has_text text="NM_001168316" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
480 <has_text text="NM_174914" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
481 <has_text text="NM_018953" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
482 <has_text text="NR_003084" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
483 <has_text text="NM_017410" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
484 <has_text text="NM_153693" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
485 <has_text text="NR_031764" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
486 <has_n_columns n="5" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
487 </assert_contents>
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
488 </output>
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
489 </test>
8
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
490 <test> <!-- bzipped input -->
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
491 <param name="single_or_paired_opts" value="paired" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
492 <param name="input_mate1" value="reads_1.fastq.bz2" ftype="fastqsanger.bz2" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
493 <param name="input_mate2" value="reads_2.fastq.bz2" ftype="fastqsanger.bz2" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
494 <param name="biasCorrect" value="False" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
495 <param name="TranscriptSource" value="history" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
496 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
497 <output name="output_quant">
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
498 <assert_contents>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
499 <has_text text="EffectiveLength" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
500 <has_text text="TPM" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
501 <has_text text="NM_001168316" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
502 <has_text text="NM_174914" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
503 <has_text text="NM_018953" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
504 <has_text text="NR_003084" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
505 <has_text text="NM_017410" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
506 <has_text text="NM_153693" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
507 <has_text text="NR_031764" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
508 <has_n_columns n="5" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
509 </assert_contents>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
510 </output>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
511 </test>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
512 <test> <!-- interleaved bz2 input -->
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
513 <param name="single_or_paired_opts" value="paired_interleaved" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
514 <param name="input_1" value="reads_both.fastq.bz2" ftype="fastqsanger.bz2" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
515 <param name="biasCorrect" value="False" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
516 <param name="TranscriptSource" value="history" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
517 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
518 <output name="output_quant">
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
519 <assert_contents>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
520 <has_text text="EffectiveLength" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
521 <has_text text="TPM" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
522 <has_text text="NM_001168316" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
523 <has_text text="NM_174914" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
524 <has_text text="NM_018953" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
525 <has_text text="NR_003084" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
526 <has_text text="NM_017410" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
527 <has_text text="NM_153693" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
528 <has_text text="NR_031764" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
529 <has_n_columns n="5" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
530 </assert_contents>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
531 </output>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
532 </test>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
533 <test> <!-- interleaved gz input -->
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
534 <param name="single_or_paired_opts" value="paired_interleaved" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
535 <param name="input_1" value="reads_both.fastq.gz" ftype="fastqsanger.gz" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
536 <param name="biasCorrect" value="False" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
537 <param name="TranscriptSource" value="history" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
538 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
539 <output name="output_quant">
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
540 <assert_contents>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
541 <has_text text="EffectiveLength" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
542 <has_text text="TPM" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
543 <has_text text="NM_001168316" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
544 <has_text text="NM_174914" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
545 <has_text text="NM_018953" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
546 <has_text text="NR_003084" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
547 <has_text text="NM_017410" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
548 <has_text text="NM_153693" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
549 <has_text text="NR_031764" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
550 <has_n_columns n="5" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
551 </assert_contents>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
552 </output>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
553 </test>
0
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
554 <test>
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
555 <param name="single_or_paired_opts" value="paired" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
556 <param name="input_mate1" value="reads_1.fastq" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
557 <param name="input_mate2" value="reads_2.fastq" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
558 <param name="TranscriptSource" value="history" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
559 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
560 <param name="geneMap" value="gene_map.tab" ftype="tabular" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
561 <output name="output_quant">
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
562 <assert_contents>
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
563 <has_text text="EffectiveLength" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
564 <has_text text="TPM" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
565 <has_text text="NM_001168316" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
566 <has_text text="NM_174914" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
567 <has_text text="NM_018953" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
568 <has_text text="NR_003084" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
569 <has_text text="NM_017410" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
570 <has_text text="NM_153693" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
571 <has_text text="NR_031764" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
572 <has_n_columns n="5" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
573 </assert_contents>
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
574 </output>
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
575 <output name="output_gene_quant">
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
576 <assert_contents>
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
577 <has_text text="EffectiveLength" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
578 <has_text text="TPM" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
579 <has_text text="baz" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
580 <has_text text="bar" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
581 <has_text text="2283" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
582 <has_text text="1640" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
583 <has_n_columns n="5" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
584 </assert_contents>
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
585 </output>
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
586 </test>
8
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
587 <test>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
588 <param name="single_or_paired_opts" value="paired_collection" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
589 <param name="input_1">
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
590 <collection type="paired">
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
591 <element name="forward" value="reads_1.fastq" ftype="fastqsanger" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
592 <element name="reverse" value="reads_2.fastq" ftype="fastqsanger" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
593 </collection>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
594 </param>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
595 <param name="TranscriptSource" value="history" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
596 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
597 <param name="geneMap" value="gene_map.tab" ftype="tabular" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
598 <output name="output_quant">
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
599 <assert_contents>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
600 <has_text text="EffectiveLength" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
601 <has_text text="TPM" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
602 <has_text text="NM_001168316" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
603 <has_text text="NM_174914" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
604 <has_text text="NM_018953" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
605 <has_text text="NR_003084" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
606 <has_text text="NM_017410" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
607 <has_text text="NM_153693" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
608 <has_text text="NR_031764" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
609 <has_n_columns n="5" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
610 </assert_contents>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
611 </output>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
612 <output name="output_gene_quant">
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
613 <assert_contents>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
614 <has_text text="EffectiveLength" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
615 <has_text text="TPM" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
616 <has_text text="baz" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
617 <has_text text="bar" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
618 <has_text text="2283" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
619 <has_text text="1640" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
620 <has_n_columns n="5" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
621 </assert_contents>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
622 </output>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
623 </test>
10
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
624 <test>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
625 <param name="single_or_paired_opts" value="paired" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
626 <param name="input_mate1" value="reads_1.fastq" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
627 <param name="input_mate2" value="reads_2.fastq" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
628 <param name="TranscriptSource" value="history" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
629 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
630 <param name="geneMap" value="gene_map.tab" ftype="tabular" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
631 <param name="validateMappings" value="True" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
632 <output name="output_quant">
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
633 <assert_contents>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
634 <has_text text="EffectiveLength" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
635 <has_text text="TPM" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
636 <has_text text="NM_001168316" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
637 <has_text text="NM_174914" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
638 <has_text text="NM_018953" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
639 <has_text text="NR_003084" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
640 <has_text text="NM_017410" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
641 <has_text text="NM_153693" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
642 <has_text text="NR_031764" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
643 <has_n_columns n="5" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
644 </assert_contents>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
645 </output>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
646 <output name="output_gene_quant">
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
647 <assert_contents>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
648 <has_text text="EffectiveLength" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
649 <has_text text="TPM" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
650 <has_text text="baz" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
651 <has_text text="bar" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
652 <has_text text="2283" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
653 <has_text text="1640" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
654 <has_n_columns n="5" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
655 </assert_contents>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
656 </output>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
657 </test>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
658 <test>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
659 <param name="single_or_paired_opts" value="paired_collection" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
660 <param name="input_1">
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
661 <collection type="paired">
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
662 <element name="forward" value="reads_1.fastq" ftype="fastqsanger" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
663 <element name="reverse" value="reads_2.fastq" ftype="fastqsanger" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
664 </collection>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
665 </param>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
666 <param name="TranscriptSource" value="history" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
667 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
668 <param name="geneMap" value="gene_map.tab" ftype="tabular" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
669 <param name="validateMappings" value="True" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
670 <output name="output_quant">
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
671 <assert_contents>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
672 <has_text text="EffectiveLength" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
673 <has_text text="TPM" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
674 <has_text text="NM_001168316" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
675 <has_text text="NM_174914" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
676 <has_text text="NM_018953" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
677 <has_text text="NR_003084" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
678 <has_text text="NM_017410" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
679 <has_text text="NM_153693" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
680 <has_text text="NR_031764" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
681 <has_n_columns n="5" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
682 </assert_contents>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
683 </output>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
684 <output name="output_gene_quant">
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
685 <assert_contents>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
686 <has_text text="EffectiveLength" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
687 <has_text text="TPM" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
688 <has_text text="baz" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
689 <has_text text="bar" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
690 <has_text text="2283" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
691 <has_text text="1640" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
692 <has_n_columns n="5" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
693 </assert_contents>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
694 </output>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
695 </test>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
696 <test>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
697 <param name="single_or_paired_opts" value="paired" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
698 <param name="input_mate1" value="reads_1.fastq" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
699 <param name="input_mate2" value="reads_2.fastq" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
700 <param name="TranscriptSource" value="history" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
701 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
702 <param name="geneMap" value="gene_map.tab" ftype="tabular" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
703 <param name="useEM" value="True" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
704 <output name="output_quant">
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
705 <assert_contents>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
706 <has_text text="EffectiveLength" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
707 <has_text text="TPM" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
708 <has_text text="NM_001168316" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
709 <has_text text="NM_174914" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
710 <has_text text="NM_018953" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
711 <has_text text="NR_003084" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
712 <has_text text="NM_017410" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
713 <has_text text="NM_153693" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
714 <has_text text="NR_031764" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
715 <has_n_columns n="5" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
716 </assert_contents>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
717 </output>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
718 <output name="output_gene_quant">
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
719 <assert_contents>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
720 <has_text text="EffectiveLength" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
721 <has_text text="TPM" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
722 <has_text text="baz" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
723 <has_text text="bar" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
724 <has_text text="2283" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
725 <has_text text="1640" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
726 <has_n_columns n="5" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
727 </assert_contents>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
728 </output>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
729 </test>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
730 <test>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
731 <param name="single_or_paired_opts" value="paired_collection" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
732 <param name="input_1">
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
733 <collection type="paired">
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
734 <element name="forward" value="reads_1.fastq" ftype="fastqsanger" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
735 <element name="reverse" value="reads_2.fastq" ftype="fastqsanger" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
736 </collection>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
737 </param>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
738 <param name="TranscriptSource" value="history" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
739 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
740 <param name="geneMap" value="gene_map.tab" ftype="tabular" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
741 <param name="useEM" value="True" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
742 <output name="output_quant">
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
743 <assert_contents>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
744 <has_text text="EffectiveLength" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
745 <has_text text="TPM" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
746 <has_text text="NM_001168316" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
747 <has_text text="NM_174914" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
748 <has_text text="NM_018953" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
749 <has_text text="NR_003084" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
750 <has_text text="NM_017410" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
751 <has_text text="NM_153693" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
752 <has_text text="NR_031764" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
753 <has_n_columns n="5" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
754 </assert_contents>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
755 </output>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
756 <output name="output_gene_quant">
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
757 <assert_contents>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
758 <has_text text="EffectiveLength" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
759 <has_text text="TPM" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
760 <has_text text="baz" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
761 <has_text text="bar" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
762 <has_text text="2283" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
763 <has_text text="1640" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
764 <has_n_columns n="5" />
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
765 </assert_contents>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
766 </output>
d9bfffca4548 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 36a1b1d98fc042a232b227f3b012e24739922b56
bgruening
parents: 9
diff changeset
767 </test>
0
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
768 </tests>
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
769
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
770 <help><![CDATA[
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
771 **What it does**
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
772 salmon is a tool for transcript quantification from RNA-seq data. It
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
773 requires a set of target transcripts (either from a reference or de-novo
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
774 assembly) to quantify. All you need to run Salmon is a fasta file containing
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
775 your reference transcripts and a (set of) fasta/fastq file(s) containing your
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
776 reads. Salmon runs in two phases; indexing and quantification. The indexing
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
777 step is independent of the reads, and only need to be run one for a particular
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
778 set of reference transcripts and choice of k (the k-mer size). The
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
779 quantification step, obviously, is specific to the set of RNA-seq reads and is
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
780 thus run more frequently.
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
781 When the quantification output contains a number of columns:
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
782 (1) Transcript ID,
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
783 (2) Transcript Length,
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
784 (3) Transcripts per Million (TPM) and
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
785 (4) Estimated number of reads (an estimate of the number of reads drawn from this transcript given the transcript’s relative abundance and length).
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
786 The first two columns are self-explanatory, the next four are measures of transcript abundance and the final is a commonly used input for differential expression tools.
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
787 The Transcripts per Million quantification number is computed as described in [1], and is meant as an estimate of the number of transcripts, per million observed transcripts,
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
788 originating from each isoform. Its benefit over the F/RPKM measure is that it is independent of the mean expressed transcript length
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
789 (i.e. if the mean expressed transcript length varies between samples, for example, this alone can affect differential analysis based on the K/RPKM.).
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
790
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
791
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
792 Fragment Library Types
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
793 ======================
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
794
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
795 There are numerous library preparation protocols for RNA-seq that result in
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diff changeset
796 sequencing reads with different characteristics. For example, reads can be
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
797 single end (only one side of a fragment is recorded as a read) or paired-end
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parents:
diff changeset
798 (reads are generated from both ends of a fragment). Further, the sequencing
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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parents:
diff changeset
799 reads themselves may be unstraned or strand-specific. Finally, paired-end
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diff changeset
800 protocols will have a specified relative orientation. To characterize the
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bgruening
parents:
diff changeset
801 various different typs of sequencing libraries, we've created a miniature
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bgruening
parents:
diff changeset
802 "language" that allows for the succinct description of the many different types
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
803 of possible fragment libraries. For paired-end reads, the possible
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
804 orientations, along with a graphical description of what they mean, are
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bgruening
parents:
diff changeset
805 illustrated below:
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parents:
diff changeset
806 .. image:: ReadLibraryIllustration.png
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bgruening
parents:
diff changeset
807 The library type string consists of three parts: the relative orientation of
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parents:
diff changeset
808 the reads, the strandedness of the library, and the directionality of the
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parents:
diff changeset
809 reads.
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parents:
diff changeset
810 The first part of the library string (relative orientation) is only provided if
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bgruening
parents:
diff changeset
811 the library is paired-end. The possible options are:
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parents:
diff changeset
812 ::
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parents:
diff changeset
813
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parents:
diff changeset
814 I = inward
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parents:
diff changeset
815 O = outward
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parents:
diff changeset
816 M = matching
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parents:
diff changeset
817
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parents:
diff changeset
818 The second part of the read library string specifies whether the protocol is
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bgruening
parents:
diff changeset
819 stranded or unstranded; the options are:
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diff changeset
820 ::
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parents:
diff changeset
821
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diff changeset
822 S = stranded
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diff changeset
823 U = unstranded
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diff changeset
824
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diff changeset
825 If the protocol is unstranded, then we're done. The final part of the library
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parents:
diff changeset
826 string specifies the strand from which the read originates in a strand-specific
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parents:
diff changeset
827 protocol — it is only provided if the library is stranded (i.e. if the
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diff changeset
828 library format string is of the form S). The possible values are:
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diff changeset
829 ::
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parents:
diff changeset
830
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diff changeset
831 F = read 1 (or single-end read) comes from the forward strand
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diff changeset
832 R = read 1 (or single-end read) comes from the reverse strand
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diff changeset
833
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diff changeset
834 So, for example, if you wanted to specify a fragment library of strand-specific
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diff changeset
835 paired-end reads, oriented toward each other, where read 1 comes from the
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diff changeset
836 forward strand and read 2 comes from the reverse strand, you would specify ``-l
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diff changeset
837 ISF`` on the command line. This designates that the library being processed has
9
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parents: 8
diff changeset
838 the type "ISF" meaning, **I**\ nward (the relative orientation), **S**\ tranded
0
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bgruening
parents:
diff changeset
839 (the protocol is strand-specific), **F**\ orward (read 1 comes from the forward
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diff changeset
840 strand).
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parents:
diff changeset
841 The single end library strings are a bit simpler than their pair-end counter
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parents:
diff changeset
842 parts, since there is no relative orientation of which to speak. Thus, the
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diff changeset
843 only possible library format types for single-end reads are ``U`` (for
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diff changeset
844 unstranded), ``SF`` (for strand-specific reads coming from the forward strand)
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diff changeset
845 and ``SR`` (for strand-specific reads coming from the reverse strand).
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diff changeset
846 A few more examples of some library format strings and their interpretations are:
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diff changeset
847 ::
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diff changeset
848
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diff changeset
849 IU (an unstranded paired-end library where the reads face each other)
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diff changeset
850
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diff changeset
851 ::
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parents:
diff changeset
852
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diff changeset
853 SF (a stranded single-end protocol where the reads come from the forward strand)
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diff changeset
854
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diff changeset
855 ::
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parents:
diff changeset
856
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diff changeset
857 OSR (a stranded paired-end protocol where the reads face away from each other,
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diff changeset
858 read1 comes from reverse strand and read2 comes from the forward strand)
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859
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diff changeset
860 .. note:: Correspondence to TopHat library types
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diff changeset
861
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diff changeset
862 The popular `TopHat <http://ccb.jhu.edu/software/tophat/index.shtml>`_ RNA-seq
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diff changeset
863 read aligner has a different convention for specifying the format of the library.
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diff changeset
864 Below is a table that provides the corresponding Salmon/salmon library format
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865 string for each of the potential TopHat library types:
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866
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diff changeset
867 +---------------------+-------------------------+
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868 | TopHat | Salmon (and Sailfish) |
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869 +=====================+============+============+
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870 | | Paired-end | Single-end |
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871 +---------------------+------------+------------+
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872 |``-fr-unstranded`` |``-l IU`` |``-l U`` |
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873 +---------------------+------------+------------+
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874 |``-fr-firststrand`` |``-l ISR`` |``-l SR`` |
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875 +---------------------+------------+------------+
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876 |``-fr-secondstrand`` |``-l ISF`` |``-l SF`` |
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877 +---------------------+------------+------------+
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878
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parents:
diff changeset
879 The remaining salmon library format strings are not directly expressible in terms
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diff changeset
880 of the TopHat library types, and so there is no direct mapping for them.
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diff changeset
881 ]]> </help>
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882 <citations>
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883 <citation type="doi">10.1101/021592</citation>
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884 </citations>
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885 </tool>