annotate salmon.xml @ 8:8c23f01f10ae draft

planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
author bgruening
date Thu, 10 May 2018 06:48:12 -0400
parents 20007d82ae2d
children 32613ec39784
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
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83bbe4e20bdc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 1b7f0370c3a5a8fd4ba2736607ad938a82a98b7b
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1 <tool id="salmon" name="Salmon" version="@VERSION@">
0
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2
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3 <description>Transcript Quantification from RNA-seq data</description>
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4
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5 <macros>
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6 <xml name="strandedness">
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7 <param name="strandedness" type="select" label="Specify the strandedness of the reads">
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8 <option value="U" selected="True">Not stranded (U)</option>
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9 <option value="SF">read 1 (or single-end read) comes from the forward strand (SF)</option>
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10 <option value="SR">read 1 (or single-end read) comes from the reverse strand (SR)</option>
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11 </param>
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12 </xml>
8
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13 <xml name="orientation">
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14 <param name="orientation" type="select" label="Relative orientation of reads within a pair">
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15 <option value="M">Mates are oriented in the same direction (M = matching)</option>
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16 <option value="O">Mates are oriented away from each other (O = outward)</option>
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17 <option value="I" selected="True">Mates are oriented toward each other (I = inward)</option>
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18 </param>
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19 </xml>
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20 <token name="@VERSION@">0.9.1</token>
0
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21 </macros>
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22
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23 <requirements>
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24 <requirement type="package" version="1.0.6">bzip2</requirement>
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25 <requirement type="package" version="@VERSION@">salmon</requirement>
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26 <requirement type="package" version="1.2">seqtk</requirement>
0
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27 </requirements>
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28
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29 <stdio>
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30 <exit_code range="1:" />
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31 <exit_code range=":-1" />
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32 <regex match="Error:" />
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33 <regex match="Exception:" />
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34 <regex match="Exception :" />
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35 </stdio>
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36 <version_command>salmon -version</version_command>
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37 <command><![CDATA[
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38 mkdir ./index
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39 &&
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40 mkdir ./output
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41 #if $refTranscriptSource.TranscriptSource == "history":
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42 &&
0
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43 salmon index
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44 --transcripts $refTranscriptSource.ownFile
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45 --kmerLen $refTranscriptSource.kmerLen
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46 --threads "\${GALAXY_SLOTS:-4}"
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47 --index './index'
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48 --type '$quasi_orphans.type'
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49 $perfectHash
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50 #if str($sasamp):
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51 --sasamp $sasamp
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52 #end if
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53 #set $index_path = './index'
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54 #else:
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55 #set $index_path = $refTranscriptSource.index.fields.path
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56 #end if
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57 &&
4
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58 #set compressed = 'no'
0
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59 #if $single_or_paired.single_or_paired_opts == 'single':
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60 #if $single_or_paired.input_singles.ext == 'fasta':
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61 #set $ext = 'fasta'
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62 #else:
8
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63 #if $single_or_paired.input_singles.is_of_type("fastq.gz", "fastqsanger.gz"):
4
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64 #set compressed = 'GZ'
8
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65 #else if $single_or_paired.input_singles.is_of_type("fastq.bz2", "fastqsanger.bz2"):
4
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66 #set compressed = 'BZ2'
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67 #end if
0
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68 #set $ext = 'fastq'
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69 #end if
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70 ln -s $single_or_paired.input_singles ./single.$ext &&
8
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71 #else if $single_or_paired.single_or_paired_opts == 'paired':
0
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72 #if $single_or_paired.input_mate1.ext == 'fasta':
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73 #set $ext = 'fasta'
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74 #else:
8
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75 #if $single_or_paired.input_mate1.is_of_type("fastq.gz", "fastqsanger.gz"):
4
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76 #set compressed = 'GZ'
8
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77 #else if $single_or_paired.input_mate1.is_of_type("fastq.bz2", "fastqsanger.bz2"):
4
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78 #set compressed = 'BZ2'
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79 #end if
0
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80 #set $ext = 'fastq'
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81 #end if
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82 ln -s $single_or_paired.input_mate1 ./mate1.$ext &&
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83 ln -s $single_or_paired.input_mate2 ./mate2.$ext &&
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84 #else if $single_or_paired.single_or_paired_opts == 'paired_collection':
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85 #if $single_or_paired.input_1.forward.ext == 'fasta':
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86 #set $ext = 'fasta'
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87 #else:
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88 #if $single_or_paired.input_1.forward.is_of_type("fastq.gz", "fastqsanger.gz"):
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89 #set compressed = 'GZ'
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90 #else if $single_or_paired.input_1.forward.is_of_type("fastq.bz2", "fastqsanger.bz2"):
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91 #set compressed = 'BZ2'
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92 #end if
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93 #set $ext = 'fastq'
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94 #end if
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95 ln -s ${single_or_paired.input_1.forward} ./mate1.$ext &&
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96 ln -s ${single_or_paired.input_1.reverse} ./mate2.$ext &&
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97 #else if $single_or_paired.single_or_paired_opts == 'paired_interleaved':
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98 #if $single_or_paired.input_1.ext == 'fasta':
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99 #set $ext = 'fasta'
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100 #else:
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101 #if $single_or_paired.input_1.is_of_type("fastq.gz", "fastqsanger.gz"):
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102 #set compressed = 'GZ'
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103 #else if $single_or_paired.input_1.is_of_type("fastq.bz2", "fastqsanger.bz2"):
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104 #set compressed = 'BZ2'
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105 #end if
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106 #set $ext = 'fastq'
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107 #end if
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108 ln -s $single_or_paired.input_1 ./mate1.$ext &&
0
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109 #end if
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110 #if $geneMap:
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111 ln -s "$geneMap" ./geneMap.${geneMap.ext} &&
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112 #end if
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113 salmon quant
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114 --index $index_path
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115 #if $single_or_paired.single_or_paired_opts == 'single':
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116 --libType ${single_or_paired.strandedness}
4
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117 #if $compressed == 'GZ':
8
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118 --unmatedReads <(zcat < ./single.$ext)
4
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119 #else if $compressed == 'BZ2':
8
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120 --unmatedReads <(bzcat < ./single.$ext)
4
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121 #else:
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122 --unmatedReads ./single.$ext
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123 #end if
0
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124 #else:
8
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125 #if $single_or_paired.single_or_paired_opts == 'paired_interleaved':
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126 #if $compressed == 'BZ2':
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127 --mates1 <(bzcat < ./mate1.$ext | seqtk seq -1)
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128 --mates2 <(bzcat < ./mate1.$ext | seqtk seq -2)
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129 #else:
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130 --mates1 <(seqtk seq -1 ./mate1.$ext)
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131 --mates2 <(seqtk seq -2 ./mate1.$ext)
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132 #end if
4
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133 #else:
8
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134 #if $compressed == 'GZ':
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135 --mates1 <(zcat < ./mate1.$ext)
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136 --mates2 <(zcat < ./mate2.$ext)
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137 #else if $compressed == 'BZ2':
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138 --mates1 <(bzcat < ./mate1.$ext)
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139 --mates2 <(bzcat < ./mate2.$ext)
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140 #else:
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141 --mates1 ./mate1.$ext
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142 --mates2 ./mate2.$ext
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143 #end if
4
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144 #end if
0
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145 --libType "${single_or_paired.orientation}${single_or_paired.strandedness}"
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146 #end if
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147 --output ./output
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148 #if str($quasi_orphans.type) == 'quasi':
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149 --allowOrphans
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150 #else:
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151 $quasi_orphans.allowOrphans
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152 #end if
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153 $seqBias
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154 $gcBias
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155 --threads "\${GALAXY_SLOTS:-4}"
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156 --incompatPrior $adv.incompatPrior
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157 $adv.consistentHits
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158 $adv.dumpEq
8
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159 $adv.reduceGCMemory
0
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160 #if str($adv.biasSpeedSamp):
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161 --biasSpeedSamp $adv.biasSpeedSamp
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162 #end if
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163 $adv.strictIntersect
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164 #if str($adv.fldMax):
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165 --fldMax $adv.fldMax
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166 #end if
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167 #if str($adv.fldMean):
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168 --fldMean $adv.fldMean
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169 #end if
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170 #if str($adv.fldSD):
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171 --fldSD $adv.fldSD
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172 #end if
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173 #if $adv.forgettingFactor:
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174 --forgettingFactor $adv.forgettingFactor
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175 #end if
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176 #if str($adv.maxOcc):
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177 --maxOcc $adv.maxOcc
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178 #end if
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179 $adv.initUniform
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180 $adv.noFragLengthDist
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181 $adv.noBiasLengthThreshold
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182 #if str($adv.maxReadOcc):
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183 --maxReadOcc $adv.maxReadOcc
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184 #end if
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185 #if $geneMap:
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186 --geneMap ./geneMap.${geneMap.ext}
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187 #end if
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188 $adv.noEffectiveLengthCorrection
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189 $adv.useVBOpt
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190 #if str($adv.numBiasSamples):
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191 --numBiasSamples $adv.numBiasSamples
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192 #end if
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193 #if str($adv.numAuxModelSamples):
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194 --numAuxModelSamples $adv.numAuxModelSamples
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195 #end if
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196 #if str($adv.numPreAuxModelSamples):
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197 --numPreAuxModelSamples $adv.numPreAuxModelSamples
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198 #end if
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199 #if str($adv.numGibbsSamples):
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200 --numGibbsSamples $adv.numGibbsSamples
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201 #end if
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202 #if str($adv.numBootstraps):
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203 --numBootstraps $adv.numBootstraps
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204 #end if
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205 $adv.perTranscriptPrior
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206 #if $adv.vbPrior:
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207 --vbPrior $adv.vbPrior
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208 #end if
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209 $adv.writeUnmappedNames
7
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210 #if str($adv.writeMappings):
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211 $adv.writeMappings > ${output_sam}
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212 #end if
0
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213 ]]>
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214 </command>
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215
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216 <inputs>
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217 <conditional name="refTranscriptSource">
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218 <param name="TranscriptSource" type="select" label="Select a reference transcriptome from your history or use a built-in index?" help="Built-ins were indexed using default options">
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219 <option value="indexed">Use a built-in index</option>
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220 <option value="history" selected="True">Use one from the history</option>
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221 </param>
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222 <when value="indexed">
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223 <param name="index" type="select" label="Select a reference transcriptome" help="If your transcriptome of interest is not listed, contact your Galaxy admin">
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224 <options from_data_table="salmon_indexes">
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225 <filter type="sort_by" column="2"/>
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226 <validator type="no_options" message="No indexes are available for the selected input dataset"/>
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227 </options>
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228 </param>
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229 </when> <!-- build-in -->
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230 <when value="history">
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231 <param name="ownFile" type="data" format="fasta" label="Select the reference transcriptome" help="in FASTA format" />
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232 <param argument="kmerLen" type="integer" value="31" label="The size should be odd number."/>
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233 </when> <!-- history -->
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234 </conditional>
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235 <conditional name="single_or_paired">
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236 <param name="single_or_paired_opts" type="select" label="Is this library mate-paired?">
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237 <option value="single">Single-end</option>
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238 <option value="paired">Paired-end</option>
8
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239 <option value="paired_collection">Paired-end Dataset Collection</option>
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240 <option value="paired_interleaved">Paired-end data from single interleaved dataset</option>
0
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241 </param>
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242 <when value="single">
8
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243 <param name="input_singles" type="data" format="fastq,fasta,fastq.gz,fastq.bz2" label="FASTQ/FASTA file" help="FASTQ file." />
0
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244 <expand macro="strandedness" />
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245 </when>
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246 <when value="paired">
8
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247 <param name="input_mate1" type="data" format="fastq,fasta,fastq.gz,fastq.bz2,fastqsanger,fastqsanger.gz,fastqsanger.bz2" label="Mate pair 1" help="FASTQ file." />
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248 <param name="input_mate2" type="data" format="fastq,fasta,fastq.gz,fastq.bz2,fastqsanger,fastqsanger.gz,fastqsanger.bz2" label="Mate pair 2" help="FASTQ file." />
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249 <expand macro="orientation" />
0
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250 <expand macro="strandedness" />
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251 </when>
8
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252 <when value="paired_collection">
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253 <param name="input_1" format="fastq,fasta,fastq.gz,fastq.bz2,fastqsanger,fastqsanger.gz,fastqsanger.bz2" type="data_collection" collection_type="paired" label="FASTQ Paired Dataset" help="Must be of datatype &quot;fastqsanger&quot; or &quot;fasta&quot;" />
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254 <expand macro="orientation" />
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255 <expand macro="strandedness" />
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256 </when>
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257 <when value="paired_interleaved">
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258 <param name="input_1" format="fastq,fasta,fastq.gz,fastq.bz2,fastqsanger,fastqsanger.gz,fastqsanger.bz2" type="data" label="Interleaved FASTQ file" help="Must be of datatype &quot;fastqsanger&quot; or &quot;fasta&quot;. --interleaved"/>
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259 <expand macro="orientation" />
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260 <expand macro="strandedness" />
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261 </when>
0
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262 </conditional>
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263 <conditional name="quasi_orphans">
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264 <param argument="--type" type="select" label="Type of index" help="When using quasi, orphaned reads will be considered when performing lightweight-alignment.">
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265 <option value="quasi" selected="True">quasi</option>
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266 <option value="fmd">fmd</option>
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267 </param>
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268 <when value="quasi">
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269 </when> <!-- build-in -->
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270 <when value="fmd">
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271 <param argument="--allowOrphans" type="boolean" truevalue="--allowOrphans" falsevalue="" checked="True"
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272 label="Consider orphaned reads as valid hits when performing lightweight-alignment"
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273 help="This option will increase sensitivity (allow more reads to map and more transcripts to be detected), but may decrease specificity as orphaned alignments are more likely to be spurious."/>
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274 </when> <!-- history -->
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275 </conditional>
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276 <param argument="--perfectHash" type="boolean" truevalue="--perfectHash" falsevalue="" checked="False"
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277 label="Perfect Hash"
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278 help="Build the index using a perfect hash rather than a dense hash. This will require less memory (especially during quantification), but will take longer to construct "/>
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279 <param argument="--sasamp" type="integer" value="1" optional="True" label="Suffix Array"
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280 help="The interval at which the suffix array should be sampled. Smaller values are faster, but produce a larger index. The default should be OK, unless your transcriptome is huge. This value should be a power of 2."/>
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281 <param argument="--seqBias" type="boolean" truevalue="--seqBias" falsevalue="" checked="False"
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282 label="Perform sequence-specific bias correction"
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283 help=""/>
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284 <param argument="--gcBias" type="boolean" truevalue="--gcBias" falsevalue="" checked="False"
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285 label="Perform fragment GC bias correction"
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286 help=""/>
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287 <param argument="--geneMap" type="data" format="tabular,gff,gtf" optional="True"
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288 label="File containing a mapping of transcripts to genes"
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289 help="If this file is provided Salmon will output both quant.sf and quant.genes.sf files, where the latter contains aggregated gene-level abundance estimates. The transcript to gene mapping should be provided as either a GTF file, or a in a simple tab-delimited format where each line contains the name of a transcript and the gene to which it belongs separated by a tab." />
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290 <section name="adv" title="Additional Options">
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291 <param argument="--writeMappings" type="boolean" truevalue="--writeMappings" falsevalue="" checked="False"
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292 label="Write Mappings"
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293 help="If this option is set to 'Yes', then the quasi-mapping results will be written out in SAM-compatible format. By default, output is directed to stdout." />
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294 <param argument="--incompatPrior" type="float" optional="True" value="9.9999999999999995e-21"
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295 label="Incompatible Prior"
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296 help="This option sets the prior probability that an alignment that disagrees with the specified library type (--libType) results from the true fragment origin. Setting this to 0 specifies that alignments that disagree with the library type should be 'impossible', while setting it to 1 says that alignments that disagree with the library type are no less likely than those that do" />
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297 <param argument="--dumpEq" type="boolean" truevalue="--dumpEq" falsevalue="" checked="False"
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298 label="Dump the equivalence class counts that were computed during quasi-mapping." help=""/>
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299 <param argument="--reduceGCMemory" type="boolean" truevalue="--reduceGCMemory" falsevalue="" optional="True" checked="False"
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300 label="If this option is selected, a more memory efficient (but slightly slower representation is used to compute fragment GC content."
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301 help="Enabling this will reduce memory usage, but can also reduce speed. However, the results themselves will remain the same."/>
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302 <param argument="--biasSpeedSamp" type="integer" value="1" optional="True"
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303 label="The value at which the fragment length PMF is down-sampled when evaluating GC fragment bias." help="Larger values speed up effective length correction, but may decrease the fidelity of bias modeling results."/>
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304 <param argument="--strictIntersect" type="boolean" truevalue="--strictIntersect" falsevalue="" checked="False"
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305 label="Modifies how orphans are assigned." help="When this flag is set, if the intersection of the quasi-mappings for the left and right is empty, then all mappings for the left and all mappings for the right read are reported as orphaned quasi-mappings."/>
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306 <param argument="--minLen" type="integer" value="19" optional="True"
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307 label=" (S)MEMs smaller than this size won't be considered." help="" />
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308 <param argument="--sensitive" type="boolean" truevalue="--sensitive" falsevalue="" checked="False"
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309 label="Perform sensitive quantification"
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310 help=" Setting this option enables the splitting of SMEMs that are larger than 1.5 times the minimum seed length (minLen/k above). This may reveal high scoring chains of MEMs that are masked by long SMEMs. However, this option makes lightweight-alignment a bit slower and is usually not necessary if the reference is of reasonable quality." />
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311 <param argument="--consistentHits" type="boolean" truevalue="--consistentHits" falsevalue="" checked="False"
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312 label="Force hits gathered during quasi-mapping to be consistent"
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313 help="" />
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314 <param argument="--extraSensitive" type="boolean" truevalue="--extraSensitive" falsevalue="" checked="False"
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315 label="Perform extra sensitive quantification"
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316 help="Setting this option enables an extra pass of 'seed' search. Enabling this option may improve sensitivity (the number of reads having sufficient coverage), but will typically slow down quantification by ~40%. Consider enabling this option if you find the mapping rate to be significantly lower than expected."/>
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317 <param argument="--coverage" type="float" value="0.69999999999999996" optional="True"
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318 label="Required coverage of read by union of SMEMs to consider it a hit"
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319 help="" />
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320 <param argument="--fldMax" type="integer" value="1000" optional="True"
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321 label="The maximum fragment length to consider when building the empirical distribution."
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322 help=""/>
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323 <param argument="--fldMean" type="integer" value="200" optional="True"
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324 label="The mean used in the fragment length distribution prior"
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325 help="If single end reads are being used for quantification, or there are an insufficient number of uniquely mapping reads when performing paired-end quantification to estimate the empirical fragment length distribution, then use this value to calculate effective lengths."/>
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326 <param argument="--fldSD" type="integer" value="80" optional="True"
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327 label="Standard deviation"
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328 help="The standard deviation used in the fragment length distribution prior."/>
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329 <param argument="--forgettingFactor" type="float" value="0.65000000000000002" optional="True"
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330 label="The forgetting factor used in the online learning schedule."
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331 help=" A smaller value results in quicker learning, but higher variance and may be unstable. A larger value results in slower learning but may be more stable. Value should be in the interval (0.5, 1.0]." />
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332 <param argument="--maxOcc" type="integer" value="200" optional="True"
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333 label="(S)MEMs occuring more than this many times won't be considered"
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334 help=""/>
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335 <param argument="--initUniform" type="boolean" truevalue="--initUniform" falsevalue="" checked="False"
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336 label="Initialization with uniform parameters"
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337 help="initialize the offline inference with uniform parameters, rather than seeding with online parameters." />
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338 <param argument="--maxReadOcc" type="integer" value="100" optional="True"
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339 label="Maximal read mapping occurence"
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340 help="Reads mapping to more than this many places won't be considered."/>
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341 <param argument="--noEffectiveLengthCorrection" type="boolean" truevalue="--noEffectiveLengthCorrection" falsevalue="" checked="False"
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342 label="Disable effective length correction"
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343 help="Disables effective length correction when computing the probability that a fragment was generated from a transcript. If this flag is passed in, the fragment length distribution is not taken into account when computing this probability."/>
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344 <param argument="--noFragLengthDist" type="boolean" truevalue="--noFragLengthDist" falsevalue="" checked="False"
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345 label="Ignore fragment length distribution"
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346 help="[experimental] : Don't consider concordance with the learned fragment length distribution when trying to determine the probability that a fragment has originated from a specified location. Normally, Fragments with unlikely lengths will be assigned a smaller relative probability than those with more likely lengths. When this flag is passed in, the observed fragment length has no effect on that fragment's a priori probability." />
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347 <param argument="--noBiasLengthThreshold" type="boolean" truevalue="--noBiasLengthThreshold" falsevalue="" checked="False"
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348 label="[experimental] : If this option is enabled, then no (lower) threshold will be set on how short bias correction can make effecctive lengths."
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349 help="This can increase the precision of bias correction, but harm robustness. The difault correction applies a threshold." />
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350 <param argument="--numBiasSamples" type="integer" value="2000000" optional="True"
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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351 label="Number of fragment mappings to use when learning the sequence-specific bias model."
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352 help="" />
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353 <param argument="--numAuxModelSamples" type="integer" value="5000000" optional="True"
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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354 label="The first numAuxModelSamples are used to train the auxiliary model parameters."
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355 help="(e.g. fragment length distribution, bias, etc.). After ther first numAuxModelSamples observations the auxiliary model parameters will be assumed to have converged and will be fixed." />
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356 <param argument="--numPreAuxModelSamples" type="integer" value="1000000" optional="True"
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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357 label="The first numPreAuxModelSamples will have their assignment likelihoods and contributions to the transcript abundances computed without applying any auxiliary models."
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358 help=" The purpose of ignoring the auxiliary models for the first numPreAuxModelSamples observations is to avoid applying these models before thier parameters have been learned sufficiently well." />
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359 <param argument="--splitWidth" type="integer" value="0" optional="True"
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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360 label=" If (S)MEM occurs fewer than this many times, search for smaller, contained MEMs"
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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361 help="The default value will not split (S)MEMs, a higher value will result in more MEMs being explore and, thus, will result in increased running time." />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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362 <param argument="--splitSpanningSeeds" type="boolean" truevalue="--splitSpanningSeeds" falsevalue="" checked="False"
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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363 label="Attempt to split seeds that happen to fall on the boundary between two transcripts."
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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364 help="This can improve the fragment hit-rate, but is usually not necessary."/>
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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365 <param argument="--useVBOpt" type="boolean" truevalue="--useVBOpt" falsevalue="" checked="False"
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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366 label="Use the Variational Bayesian EM rather than the traditional EM algorithm for optimization in the batch passes."
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diff changeset
367 help=""/>
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368 <param argument="--numGibbsSamples" type="integer" value="0" optional="True"
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369 label=" Number of Gibbs sampling rounds to perform."
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370 help="" />
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371 <param argument="--numBootstraps" type="integer" value="0" optional="True"
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372 label="Number of bootstrap samples to generate. Note: This is mutually exclusive with Gibbs sampling."
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373 help="" />
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374 <param argument="--perTranscriptPrior" type="boolean" truevalue="--perTranscriptPrior" falsevalue="" checked="False"
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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375 label="The prior will be interpreted as a transcript-level prior."
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376 help="either the default or the argument provided via --vbPrior" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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377 <param argument="--vbPrior" type="float" value="0.001" optional="True"
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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378 label="The prior that will be used in the VBEM algorithm."
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379 help="This is interpreted as a per-nucleotide prior, unless the --perTranscriptPrior flag is also given, in which case this is used as a transcript-level prior." />
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380 <param argument="--writeUnmappedNames" type="boolean" truevalue="--writeUnmappedNames" falsevalue="" checked="False"
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381 label="Write the names of un-mapped reads to the file unmapped_names.txt."
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diff changeset
382 help=""/>
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diff changeset
383 </section>
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diff changeset
384 </inputs>
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diff changeset
385
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
386 <outputs>
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387 <data name="output_quant" format="tabular" from_work_dir="output/quant.sf" label="${tool.name} on ${on_string} (Quantification)" />
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388 <data name="output_gene_quant" format="tabular" from_work_dir="output/quant.genes.sf" label="${tool.name} on ${on_string} (Gene Quantification)">
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
389 <filter>geneMap</filter>
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390 </data>
7
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diff changeset
391 <data name="output_sam" format="sam" label="${tool.name} on ${on_string} (SAM format)">
20007d82ae2d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 3103ebed1a420c7d3415b67ef532ea579edf9faa
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diff changeset
392 <filter>adv['writeMappings']</filter>
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diff changeset
393 </data>
0
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
394 </outputs>
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diff changeset
395
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
396 <tests>
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diff changeset
397 <test>
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398 <param name="single_or_paired_opts" value="paired" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
399 <param name="input_mate1" value="reads_1.fastq" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
400 <param name="input_mate2" value="reads_2.fastq" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
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diff changeset
401 <param name="biasCorrect" value="False" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
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diff changeset
402 <param name="TranscriptSource" value="history" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
403 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
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diff changeset
404 <output name="output_quant">
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
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diff changeset
405 <assert_contents>
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406 <has_text text="EffectiveLength" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
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diff changeset
407 <has_text text="TPM" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
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diff changeset
408 <has_text text="NM_001168316" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
409 <has_text text="NM_174914" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
410 <has_text text="NM_018953" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
411 <has_text text="NR_003084" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
412 <has_text text="NM_017410" />
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diff changeset
413 <has_text text="NM_153693" />
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414 <has_text text="NR_031764" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
415 <has_n_columns n="5" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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416 </assert_contents>
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417 </output>
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418 </test>
4
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419 <test> <!-- gzipped input -->
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420 <param name="single_or_paired_opts" value="paired" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
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421 <param name="input_mate1" value="reads_1.fastq.gz" ftype="fastqsanger.gz" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
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422 <param name="input_mate2" value="reads_2.fastq.gz" ftype="fastqsanger.gz" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
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423 <param name="biasCorrect" value="False" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
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424 <param name="TranscriptSource" value="history" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
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425 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
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426 <output name="output_quant">
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427 <assert_contents>
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
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428 <has_text text="EffectiveLength" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
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diff changeset
429 <has_text text="TPM" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
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diff changeset
430 <has_text text="NM_001168316" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
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diff changeset
431 <has_text text="NM_174914" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
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diff changeset
432 <has_text text="NM_018953" />
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diff changeset
433 <has_text text="NR_003084" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
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diff changeset
434 <has_text text="NM_017410" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
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diff changeset
435 <has_text text="NM_153693" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
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diff changeset
436 <has_text text="NR_031764" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
437 <has_n_columns n="5" />
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
438 </assert_contents>
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
439 </output>
979bff04ae7d planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 359ddec398e18d3e2a534239b1202691595d1243
bgruening
parents: 3
diff changeset
440 </test>
8
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
441 <test> <!-- bzipped input -->
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
442 <param name="single_or_paired_opts" value="paired" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
443 <param name="input_mate1" value="reads_1.fastq.bz2" ftype="fastqsanger.bz2" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
444 <param name="input_mate2" value="reads_2.fastq.bz2" ftype="fastqsanger.bz2" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
445 <param name="biasCorrect" value="False" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
446 <param name="TranscriptSource" value="history" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
447 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
448 <output name="output_quant">
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
449 <assert_contents>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
450 <has_text text="EffectiveLength" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
451 <has_text text="TPM" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
452 <has_text text="NM_001168316" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
453 <has_text text="NM_174914" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
454 <has_text text="NM_018953" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
455 <has_text text="NR_003084" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
456 <has_text text="NM_017410" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
457 <has_text text="NM_153693" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
458 <has_text text="NR_031764" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
459 <has_n_columns n="5" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
460 </assert_contents>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
461 </output>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
462 </test>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
463 <test> <!-- interleaved bz2 input -->
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
464 <param name="single_or_paired_opts" value="paired_interleaved" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
465 <param name="input_1" value="reads_both.fastq.bz2" ftype="fastqsanger.bz2" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
466 <param name="biasCorrect" value="False" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
467 <param name="TranscriptSource" value="history" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
468 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
469 <output name="output_quant">
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
470 <assert_contents>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
471 <has_text text="EffectiveLength" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
472 <has_text text="TPM" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
473 <has_text text="NM_001168316" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
474 <has_text text="NM_174914" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
475 <has_text text="NM_018953" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
476 <has_text text="NR_003084" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
477 <has_text text="NM_017410" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
478 <has_text text="NM_153693" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
479 <has_text text="NR_031764" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
480 <has_n_columns n="5" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
481 </assert_contents>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
482 </output>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
483 </test>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
484 <test> <!-- interleaved gz input -->
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
485 <param name="single_or_paired_opts" value="paired_interleaved" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
486 <param name="input_1" value="reads_both.fastq.gz" ftype="fastqsanger.gz" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
487 <param name="biasCorrect" value="False" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
488 <param name="TranscriptSource" value="history" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
489 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
490 <output name="output_quant">
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
491 <assert_contents>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
492 <has_text text="EffectiveLength" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
493 <has_text text="TPM" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
494 <has_text text="NM_001168316" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
495 <has_text text="NM_174914" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
496 <has_text text="NM_018953" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
497 <has_text text="NR_003084" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
498 <has_text text="NM_017410" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
499 <has_text text="NM_153693" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
500 <has_text text="NR_031764" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
501 <has_n_columns n="5" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
502 </assert_contents>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
503 </output>
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
bgruening
parents: 7
diff changeset
504 </test>
0
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
505 <test>
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
506 <param name="single_or_paired_opts" value="paired" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
507 <param name="input_mate1" value="reads_1.fastq" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
508 <param name="input_mate2" value="reads_2.fastq" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
509 <param name="TranscriptSource" value="history" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
510 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
511 <param name="geneMap" value="gene_map.tab" ftype="tabular" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
512 <output name="output_quant">
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
513 <assert_contents>
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
514 <has_text text="EffectiveLength" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
515 <has_text text="TPM" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
516 <has_text text="NM_001168316" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
517 <has_text text="NM_174914" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
518 <has_text text="NM_018953" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
519 <has_text text="NR_003084" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
520 <has_text text="NM_017410" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
521 <has_text text="NM_153693" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
522 <has_text text="NR_031764" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
523 <has_n_columns n="5" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
524 </assert_contents>
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
525 </output>
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
parents:
diff changeset
526 <output name="output_gene_quant">
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parents:
diff changeset
527 <assert_contents>
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parents:
diff changeset
528 <has_text text="EffectiveLength" />
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parents:
diff changeset
529 <has_text text="TPM" />
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parents:
diff changeset
530 <has_text text="baz" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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parents:
diff changeset
531 <has_text text="bar" />
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parents:
diff changeset
532 <has_text text="2283" />
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parents:
diff changeset
533 <has_text text="1640" />
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parents:
diff changeset
534 <has_n_columns n="5" />
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
535 </assert_contents>
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parents:
diff changeset
536 </output>
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parents:
diff changeset
537 </test>
8
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parents: 7
diff changeset
538 <test>
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parents: 7
diff changeset
539 <param name="single_or_paired_opts" value="paired_collection" />
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diff changeset
540 <param name="input_1">
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
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diff changeset
541 <collection type="paired">
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542 <element name="forward" value="reads_1.fastq" ftype="fastqsanger" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
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diff changeset
543 <element name="reverse" value="reads_2.fastq" ftype="fastqsanger" />
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diff changeset
544 </collection>
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diff changeset
545 </param>
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diff changeset
546 <param name="TranscriptSource" value="history" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
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547 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
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diff changeset
548 <param name="geneMap" value="gene_map.tab" ftype="tabular" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
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549 <output name="output_quant">
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diff changeset
550 <assert_contents>
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diff changeset
551 <has_text text="EffectiveLength" />
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parents: 7
diff changeset
552 <has_text text="TPM" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
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parents: 7
diff changeset
553 <has_text text="NM_001168316" />
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diff changeset
554 <has_text text="NM_174914" />
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555 <has_text text="NM_018953" />
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diff changeset
556 <has_text text="NR_003084" />
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diff changeset
557 <has_text text="NM_017410" />
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diff changeset
558 <has_text text="NM_153693" />
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diff changeset
559 <has_text text="NR_031764" />
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560 <has_n_columns n="5" />
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561 </assert_contents>
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diff changeset
562 </output>
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563 <output name="output_gene_quant">
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564 <assert_contents>
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diff changeset
565 <has_text text="EffectiveLength" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
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parents: 7
diff changeset
566 <has_text text="TPM" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
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diff changeset
567 <has_text text="baz" />
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diff changeset
568 <has_text text="bar" />
8c23f01f10ae planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit e42ab88387f117f726cfa874c6232afd2c844c86
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diff changeset
569 <has_text text="2283" />
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570 <has_text text="1640" />
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diff changeset
571 <has_n_columns n="5" />
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572 </assert_contents>
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diff changeset
573 </output>
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diff changeset
574 </test>
0
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575 </tests>
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576
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
577 <help><![CDATA[
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diff changeset
578 **What it does**
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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579 salmon is a tool for transcript quantification from RNA-seq data. It
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
580 requires a set of target transcripts (either from a reference or de-novo
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
581 assembly) to quantify. All you need to run Salmon is a fasta file containing
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
582 your reference transcripts and a (set of) fasta/fastq file(s) containing your
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
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diff changeset
583 reads. Salmon runs in two phases; indexing and quantification. The indexing
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
584 step is independent of the reads, and only need to be run one for a particular
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
bgruening
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diff changeset
585 set of reference transcripts and choice of k (the k-mer size). The
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
586 quantification step, obviously, is specific to the set of RNA-seq reads and is
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
587 thus run more frequently.
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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588 When the quantification output contains a number of columns:
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
589 (1) Transcript ID,
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
590 (2) Transcript Length,
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
591 (3) Transcripts per Million (TPM) and
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
592 (4) Estimated number of reads (an estimate of the number of reads drawn from this transcript given the transcript’s relative abundance and length).
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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593 The first two columns are self-explanatory, the next four are measures of transcript abundance and the final is a commonly used input for differential expression tools.
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594 The Transcripts per Million quantification number is computed as described in [1], and is meant as an estimate of the number of transcripts, per million observed transcripts,
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
595 originating from each isoform. Its benefit over the F/RPKM measure is that it is independent of the mean expressed transcript length
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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596 (i.e. if the mean expressed transcript length varies between samples, for example, this alone can affect differential analysis based on the K/RPKM.).
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diff changeset
597
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diff changeset
598
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
599 Fragment Library Types
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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600 ======================
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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601
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
602 There are numerous library preparation protocols for RNA-seq that result in
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
603 sequencing reads with different characteristics. For example, reads can be
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
604 single end (only one side of a fragment is recorded as a read) or paired-end
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
605 (reads are generated from both ends of a fragment). Further, the sequencing
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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606 reads themselves may be unstraned or strand-specific. Finally, paired-end
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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607 protocols will have a specified relative orientation. To characterize the
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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608 various different typs of sequencing libraries, we've created a miniature
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609 "language" that allows for the succinct description of the many different types
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610 of possible fragment libraries. For paired-end reads, the possible
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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diff changeset
611 orientations, along with a graphical description of what they mean, are
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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612 illustrated below:
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613 .. image:: ReadLibraryIllustration.png
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614 The library type string consists of three parts: the relative orientation of
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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615 the reads, the strandedness of the library, and the directionality of the
22cb19d12fcc planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/salmon commit 49aa400dbbcab6ce76272d8b05df6981a5307783-dirty
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616 reads.
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617 The first part of the library string (relative orientation) is only provided if
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618 the library is paired-end. The possible options are:
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619 ::
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620
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621 I = inward
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622 O = outward
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623 M = matching
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624
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625 The second part of the read library string specifies whether the protocol is
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626 stranded or unstranded; the options are:
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627 ::
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628
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629 S = stranded
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630 U = unstranded
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631
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632 If the protocol is unstranded, then we're done. The final part of the library
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633 string specifies the strand from which the read originates in a strand-specific
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634 protocol — it is only provided if the library is stranded (i.e. if the
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635 library format string is of the form S). The possible values are:
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636 ::
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637
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638 F = read 1 (or single-end read) comes from the forward strand
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639 R = read 1 (or single-end read) comes from the reverse strand
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640
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641 So, for example, if you wanted to specify a fragment library of strand-specific
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642 paired-end reads, oriented toward each other, where read 1 comes from the
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643 forward strand and read 2 comes from the reverse strand, you would specify ``-l
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644 ISF`` on the command line. This designates that the library being processed has
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645 the type "ISF" meaning, **I**\ nward (the relative orientation), **S**\ tranted
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646 (the protocol is strand-specific), **F**\ orward (read 1 comes from the forward
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647 strand).
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648 The single end library strings are a bit simpler than their pair-end counter
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649 parts, since there is no relative orientation of which to speak. Thus, the
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650 only possible library format types for single-end reads are ``U`` (for
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651 unstranded), ``SF`` (for strand-specific reads coming from the forward strand)
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652 and ``SR`` (for strand-specific reads coming from the reverse strand).
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653 A few more examples of some library format strings and their interpretations are:
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654 ::
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655
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656 IU (an unstranded paired-end library where the reads face each other)
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657
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658 ::
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diff changeset
659
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660 SF (a stranded single-end protocol where the reads come from the forward strand)
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661
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662 ::
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663
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664 OSR (a stranded paired-end protocol where the reads face away from each other,
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665 read1 comes from reverse strand and read2 comes from the forward strand)
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diff changeset
666
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667 .. note:: Correspondence to TopHat library types
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668
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669 The popular `TopHat <http://ccb.jhu.edu/software/tophat/index.shtml>`_ RNA-seq
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670 read aligner has a different convention for specifying the format of the library.
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671 Below is a table that provides the corresponding Salmon/salmon library format
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672 string for each of the potential TopHat library types:
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673
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674 +---------------------+-------------------------+
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675 | TopHat | Salmon (and Sailfish) |
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676 +=====================+============+============+
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677 | | Paired-end | Single-end |
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678 +---------------------+------------+------------+
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679 |``-fr-unstranded`` |``-l IU`` |``-l U`` |
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680 +---------------------+------------+------------+
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681 |``-fr-firststrand`` |``-l ISR`` |``-l SR`` |
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682 +---------------------+------------+------------+
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683 |``-fr-secondstrand`` |``-l ISF`` |``-l SF`` |
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684 +---------------------+------------+------------+
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685
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686 The remaining salmon library format strings are not directly expressible in terms
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687 of the TopHat library types, and so there is no direct mapping for them.
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688 ]]> </help>
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689 <citations>
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690 <citation type="doi">10.1101/021592</citation>
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691 </citations>
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692 </tool>