Mercurial > repos > artbio > rsem
changeset 5:78a1909aca3e draft
planemo upload for repository https://github.com/artbio/tools-artbio/tree/master/tools/rsem commit f517d1dbd9f4c6fd0b37317555315c60a09451ee
| author | artbio |
|---|---|
| date | Sat, 31 Mar 2018 10:17:42 -0400 |
| parents | 0fa0c1132a7b |
| children | 39b8e13b1fc9 |
| files | macros.xml rsem-bwt2.xml rsem.xml test-data/bowtie-alignment.tab test-data/gene_abundances.tab2 test-data/isoform_abundances.tab2 |
| diffstat | 6 files changed, 1633 insertions(+), 1115 deletions(-) [+] |
line wrap: on
line diff
--- a/macros.xml Wed Mar 28 10:43:28 2018 -0400 +++ b/macros.xml Sat Mar 31 10:17:42 2018 -0400 @@ -69,7 +69,34 @@ </when> </conditional> </xml> - <xml name="sampling_for_bam"> + <xml name="bowtie2_options"> + <conditional name="bowtie2_options"> + <param name="fullparams" type="select" label="bowtie settings"> + <option value="default">use bowtie2 defaults</option> + <option value="fullset">set bowtie2 options</option> + </param> + <when value="default"/> + <when value="fullset"> + <param name="bowtie2_mismatch_rate" type="float" value="0.1" label="--bowtie2-mismatch-rate" help="The maximum mismatch rate allowed. (Default: 0.1)"> + <validator type="in_range" message="a rate, between 0 and 0.8" min="0" max="0.8"/> + </param> + <param name="bowtie2_k" type="integer" value="200" label="--bowtie2-k" help="Find up to `int` alignments per read. (Default: 200)"> + </param> + <param name="bowtie2_sensitivity_level" type="select" label="--bowtie2-sensitivity-level"> + <option value="very_fast">very_fast</option> + <option value="fast">fast</option> + <option value="sensitive" selected="true">sensitive</option> + <option value="very_sensitive">very_sensitive</option> + <help> + Set Bowtie 2's preset options in --end-to-end mode. This option controls how hard Bowtie 2 tries to find + alignments. Must be one of "very_fast", "fast", "sensitive" and "very_sensitive". The four candidates correspond to Bowtie 2's + "--very-fast", "--fast", "--sensitive" and "--very-sensitive" options. (Default: "sensitive" - use Bowtie 2's default) + </help> + </param> + </when> + </conditional> + </xml> + <xml name="sampling_for_bam"> <param name="sampling_for_bam" type="boolean" truevalue="--sampling-for-bam" falsevalue="" checked="false" label="Use sampling for BAM"> <help> When RSEM generates a BAM file, instead of outputing all alignments a read has with their posterior probabilities, one alignment is sampled according to the posterior probabilities. The sampling procedure includes the alignment to the "noise" transcript, which does not appear in the BAM file. Only the sampled alignment has a weight of 1. All other alignments have weight 0. If the "noise" transcript is sampled, all alignments appeared in the BAM file should have weight 0. (Default: off) </help>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/rsem-bwt2.xml Sat Mar 31 10:17:42 2018 -0400 @@ -0,0 +1,1426 @@ +<tool id="rsembowtie2" name="RSEM-Bowtie2" version="0.4.0"> + <description></description> + <macros> + <import>macros.xml</import> + </macros> + <requirements> + <requirement type="package" version="1.3.0">rsem</requirement> + <requirement type="package" version="2.3.4">bowtie2</requirement> + </requirements> + <stdio> + <exit_code range="1:" level="warning" description="Tool exception" /> + </stdio> + <command detect_errors="exit_code"><![CDATA[ + #if $job.select_job == "index": + echo ${job.reference_name} " " | tee $reference_file && + mkdir $reference_file.files_path && + rsem-prepare-reference + #if $job.polya.polya_use == 'add': + #if $job.polya.polya_length: + --polyA-length $job.polya.polya_length + #end if + #elif $job.polya.polya_use == 'subset': + --no-polyA-subset $job.polya.no_polya_subset + #if $job.polya.polya_length: + --polyA-length $job.polya.polya_length + #end if + #elif $job.polya.polya_use == 'none': + --no-polyA + #end if + $job.ntog + #if $job.transcript_to_gene_map: + --transcript-to-gene-map $job.transcript_to_gene_map + #end if + --bowtie2 + #if $job.self_reference.ref_type == 'transcripts': + $job.self_reference.reference_fasta_file + #else: + --gtf $job.self_reference.gtf + $job.self_reference.reference_fasta_file + #end if + ${reference_file.files_path}/${job.reference_name} + > ${reference_file.files_path}/${job.reference_name}.log + #end if + + #if $job.select_job == "index" and $run_rsem.select == "Yes": + && + #end if + + #if $run_rsem.select == "Yes": + ## uncompress fastq.gz or fastqsanger.gz if needed + #if $run_rsem.input.fastq.matepair=="single": + #if $run_rsem.input.fastq.singlefastq.is_of_type('fastq.gz') or $run_rsem.input.fastq.singlefastq.is_of_type('fastqsanger.gz'): + gunzip < '$run_rsem.input.fastq.singlefastq' > uncomp_single.fastq && + #elif $run_rsem.input.fastq.singlefastq.is_of_type('fastq') or $run_rsem.input.fastq.singlefastq.is_of_type('fastqsanger'): + ln -f -s '$run_rsem.input.fastq.singlefastq' 'uncomp_single.fastq' && + #end if + #elif $run_rsem.input.fastq.matepair=="paired": + #if $run_rsem.input.fastq.fastq1.is_of_type('fastq.gz') or $run_rsem.input.fastq.fastq1.is_of_type('fastqsanger.gz'): + gunzip < '$run_rsem.input.fastq.fastq1' > uncomp_pair1.fastq && + gunzip < '$run_rsem.input.fastq.fastq2' > uncomp_pair2.fastq && + #elif $run_rsem.input.fastq.singlefastq.is_of_type('fastq') or $run_rsem.input.fastq.singlefastq.is_of_type('fastqsanger'): + ln -f -s '$run_rsem.input.fastq.fastq1' 'uncomp_pair1.fastq' && + ln -f -s '$run_rsem.input.fastq.fastq2' 'uncomp_pair2.fastq' && + #end if + #end if + rsem-calculate-expression + ## --tag string + #if $run_rsem.seedlength: + --seed-length $run_rsem.seedlength + #end if + --forward-prob $run_rsem.forward_prob + #if $run_rsem.rsem_options.fullparams == 'fullset': + ## Fragment info + #if $run_rsem.rsem_options.fragment_length_mean: + --fragment-length-mean $run_rsem.rsem_options.fragment_length_mean + #end if + #if $run_rsem.rsem_options.fragment_length_min: + --fragment-length-min $run_rsem.rsem_options.fragment_length_min + #end if + #if $run_rsem.rsem_options.fragment_length_sd: + --fragment-length-sd $run_rsem.rsem_options.fragment_length_sd + #end if + #if $run_rsem.rsem_options.fragment_length_max: + --fragment-length-max $run_rsem.rsem_options.fragment_length_max + #end if + ## RSPD + #if $run_rsem.rsem_options.rspd.estimate == 'yes': + --estimate-rspd + #if $run_rsem.rsem_options.rspd.num_rspd_bins: + --num-rspd-bins $run_rsem.rsem_options.rspd.num_rspd_bins + #end if + #end if + ## Calculate 95% credibility intervals and posterior mean estimates. + #if $run_rsem.rsem_options.useci.ci == 'yes': + --calc-ci + #if $run_rsem.rsem_options.useci.cimem: + --ci-memory $run_rsem.rsem_options.useci.cimem + #end if + #end if + #end if + --num-threads \${GALAXY_SLOTS:-4} + --bowtie2 + #if $run_rsem.input.format == 'fasta' and $run_rsem.input.bowtie2_options.fullparams == 'fullset': + ## Bowtie params + #if $run_rsem.input.bowtie2_options.bowtie2_mismatch_rate: + --bowtie2-mismatch-rate $run_rsem.input.bowtie2_options.bowtie2_mismatch_rate + #end if + #if $run_rsem.input.bowtie2_options.bowtie2_k: + --bowtie2-k $run_rsem.input.bowtie2_options.bowtie2_k + #end if + #if $run_rsem.input.bowtie2_options.bowtie2_sensitivity_level: + --bowtie2-sensitivity-level $run_rsem.input.bowtie2_options.bowtie2_sensitivity_level + #end if + #end if + ## Outputs + #if $run_rsem.rsem_outputs.result_bams == 'none': + --no-bam-output + #elif $run_rsem.rsem_outputs.result_bams == 'default': + --sort-bam-by-coordinate + #else + --sort-bam-by-coordinate + --output-genome-bam + $run_rsem.rsem_outputs.sampling_for_bam + #end if + ## Input data + #if $run_rsem.input.format=="fastq" + $run_rsem.input.fastq_select + #if $run_rsem.input.fastq.matepair=="single": + uncomp_single.fastq + #elif $run_rsem.input.fastq.matepair=="paired": + --paired-end + uncomp_pair1.fastq + uncomp_pair2.fastq + #end if + #elif $run_rsem.input.format=="fasta" + --no-qualities + #if $run_rsem.input.fasta.matepair=="single": + $run_rsem.input.fasta.singlefasta + #elif $run_rsem.input.fasta.matepair=="paired": + --paired-end + $run_rsem.input.fasta.fasta1 + $run_rsem.input.fasta.fasta2 + #end if + #elif $run_rsem.input.format=="sam" + #if $run_rsem.input.matepair=="paired": + --paired-end + #end if + #if $run_rsem.input.rsem_sam._extension == 'sam': + --sam + #elif $run_rsem.input.rsem_sam._extension == 'bam': + --bam + #end if + $run_rsem.input.rsem_sam + #end if + ## RSEM reference + #if $run_rsem.reference.refSrc == 'history': + ${run_rsem.reference.rsem_ref.extra_files_path}/${run_rsem.reference.rsem_ref.metadata.reference_name} + #elif $run_rsem.reference.refSrc == 'self': + ${reference_file.files_path}/${job.reference_name} + #end if + ## sample_name: use a hard coded name so we can pull out galaxy outputs + rsem_output + ## direct output into logfile + > $log + #end if + ]]></command> + + <inputs> + <conditional name="job"> + <param name="select_job" type="select" label="rsem reference"> + <option value="index">Build rsem reference</option> + <option value="no-index" selected="true">rsem reference available from history</option> + </param> + <when value="index"> + <conditional name="self_reference"> + <param name="ref_type" type="select" label="Reference transcript source"> + <option value="transcripts">transcript fasta</option> + <option value="genomic">reference genome and gtf</option> + </param> + <when value="transcripts"> + <param name="reference_fasta_file" type="data" format="fasta" label="reference fasta file" + help="The files should contain the sequences of transcripts."/> + </when> + <when value="genomic"> + <param name="reference_fasta_file" type="data" format="fasta" label="reference fasta file" + help="The file should contain the sequence of an entire genome."/> + <param name="gtf" type="data" format="gtf" label="gtf" + help="extract transcript reference sequences using the gene annotations specified in this GTF" /> + </when> + </conditional> + <param name="transcript_to_gene_map" type="data" format="tabular" optional="true" label="Map of gene ids to transcript (isoform) ids" > + <help> + Each line of should be of the form: gene_id transcript_id ( with the two fields separated by a tab character ) + The map can be obtained from the UCSC table browser + group: Genes and Gene Prediction Tracks + table: knownIsoforms + Without a map: + If a reference genome and gtf is used, then RSEM uses the "gene_id" and "transcript_id" attributes in the GTF file. + Otherwise, RSEM assumes that each sequence in the reference sequence files is a separate gene. + </help> + </param> + <param name="reference_name" type="text" value="rsem_ref_name" label="reference name"> + <help>A one word name for this RSEM reference containing only letters, digits, and underscore characters</help> + <validator type="regex" message="Use only letters, digits, and underscore characters">^\w+$</validator> + </param> + <conditional name="polya"> + <param name="polya_use" type="select" label="PolyA "> + <option value="add" selected="true">Add poly(A) tails to all transcripts</option> + <option value="subset">Exclude poly(A) tails from selected transcripts</option> + <option value="none">Do not add poly(A) tails to any transcripts</option> + </param> + <when value="add"> + <param name="polya_length" type="integer" value="125" optional="true" label="The length of the poly(A) tails to be added. (Default: 125)"> + <validator type="in_range" message="must be positive " min="1"/> + </param> + </when> + <when value="subset"> + <param name="no_polya_subset" type="data" format="tabular" optional="true" label="List of transcript IDs (one per line) that should should not have polyA tails added."/> + <param name="polya_length" type="integer" value="125" optional="true" label="The length of the poly(A) tails to be added. (Default: 125)"> + <validator type="in_range" message="must be positive " min="1"/> + </param> + </when> + <when value="none"/> + </conditional> + <param name="ntog" type="boolean" truevalue="--no-ntog" falsevalue="" checked="false" label="Disable the conversion of 'N' characters to 'G' characters in the reference sequences" help="Bowtie uses the automatic N to G conversion to align against all positions in the reference."/> + </when> + <when value="no-index"> + </when> + </conditional> + + <conditional name="run_rsem"> + <param name="select" type="select" label="calculate expression with rsem"> + <option value="No">Just build rsem reference for latter rsem profiling</option> + <option value="Yes" selected="true">profile expression with rsem</option> + </param> + <when value="Yes"> + <param name="sample" type="text" value="rsem_sample" label="Sample name" /> + <conditional name="reference"> + <param name="refSrc" type="select" label="RSEM Reference Source"> + <option value="history">From your history</option> + <option value="self">Prepare RSEM Reference with this tool</option> + </param> + <when value="history"> + <param name="rsem_ref" type="data" format="rsem_ref" label="RSEM reference" /> + </when> + <when value="self"> + </when> + </conditional> + <conditional name="input"> + <param name="format" type="select" label="RSEM Input file type"> + <option value="fastq">FASTQ</option> + <option value="fasta">FASTA</option> + <option value="sam">SAM/BAM</option> + </param> + <when value="fastq"> + <param name="fastq_select" size="15" type="select" label="FASTQ type" > + <option value="--phred33-quals" selected="true">phred33 qualities (default for sanger)</option> + <option value="--solexa-quals">solexa qualities</option> + <option value="--phred64-quals">phred64 qualities</option> + </param> + <conditional name="fastq"> + <param name="matepair" type="select" label="Library type"> + <option value="single">Single End Reads</option> + <option value="paired">Paired End Reads</option> + </param> + <when value="single"> + <param name="singlefastq" type="data" format="fastq,fastq.gz" label="FASTQ file" /> + </when> + <when value="paired"> + <param name="fastq1" type="data" format="fastq,fastq.gz" label="Read 1 fastq file" /> + <param name="fastq2" type="data" format="fastq,fastq.gz" label="Read 2 fastq file" /> + </when> + </conditional> + <expand macro="bowtie2_options"/> + </when> + <when value="fasta"> + <conditional name="fasta"> + <param name="matepair" type="select" label="Library Type"> + <option value="single">Single End Reads</option> + <option value="paired">Paired End Reads</option> + </param> + <when value="single"> + <param name="singlefasta" type="data" format="fasta" label="fasta file" /> + </when> + <when value="paired"> + <param name="fasta1" type="data" format="fasta" label="Read 1 fasta file" /> + <param name="fasta2" type="data" format="fasta" label="Read 2 fasta file" /> + </when> + </conditional> + <expand macro="bowtie2_options"/> + </when> + <when value="sam"> + <!-- convert-sam-for-rsem /ref/mouse_125 input.sam -o input_for_rsem.sam --> + <param name="matepair" type="select" label="Library Type"> + <option value="single">Single End Reads</option> + <option value="paired">Paired End Reads</option> + </param> + <param name="rsem_sam" type="data" format="rsem_sam" label="RSEM formatted SAM file" /> + </when> + </conditional> + <expand macro="rsem_options"/> + <conditional name="rsem_outputs"> + <param name="result_bams" type="select" label="Create bam results files" + help="In addition to the transcript-coordinate-based BAM file output, also output a BAM file with the read alignments in genomic coordinates" > + <option value="none">No BAM results files</option> + <option value="default" selected="true">Transcript BAM results file</option> + <option value="both">Transcript and genome BAM results files</option> + </param> + <when value="none"/> + <when value="default"> + <expand macro="sampling_for_bam"/> + </when> + <when value="both"> + <expand macro="sampling_for_bam"/> + </when> + </conditional> + </when> + <when value="No"> + </when> + </conditional> + </inputs> + + <outputs> + <data format="rsem_ref" name="reference_file" label="RSEM ${job.reference_name} reference"> + <filter>job['select_job'] == "index"</filter> + </data> + <data format="tabular" name="gene_abundances" label="${run_rsem.sample}.gene_abundances" from_work_dir="rsem_output.genes.results"> + <filter>run_rsem['select'] == "Yes"</filter> + </data> + <data format="tabular" name="isoform_abundances" label="${run_rsem.sample}.isoform_abundances" from_work_dir="rsem_output.isoforms.results"> + <filter>run_rsem['select'] == "Yes"</filter> + </data> + <data format="bam" name="transcript_sorted_bam" label="${run_rsem.sample}.transcript.bam" from_work_dir="rsem_output.transcript.sorted.bam" > + <filter>run_rsem['select'] == "Yes" and run_rsem['rsem_outputs']['result_bams'] != "none"</filter> + </data> + <data format="bam" name="genome_sorted_bam" label="${run_rsem.sample}.genome.bam" from_work_dir="rsem_output.genome.sorted.bam"> + <filter>run_rsem['select'] == "Yes" and run_rsem['rsem_outputs']['result_bams'] == "both"</filter> + </data> + <data format="txt" name="log" label="${run_rsem.sample}.rsem_log"> + <filter>run_rsem['select'] == "Yes"</filter> + </data> + </outputs> + + <tests> + <test> + <param name="select_job" value="index"/> + <param name="ref_type" value="genomic"/> + <param name="reference_fasta_file" value="ref.fasta" ftype="fasta"/> + <param name="gtf" value="ref.gtf" ftype="gtf"/> + <param name="reference_name" value="ref"/> + <param name="select" value="Yes"/> + <param name="sample" value="rsem_sample"/> + <param name="refSrc" value="self"/> + <param name="format" value="fastq"/> + <param name="matepair" value="single"/> + <param name="singlefastq" value="test.fq" ftype="fastqsanger"/> + <param name="result_bams" value="none"/> + <output name="reference_file"> + <assert_contents> + <has_text text="ref" /> + </assert_contents> + </output> + <output name="gene_abundances" value="gene_abundances.tab2"/> + <output name="isoform_abundances" value="isoform_abundances.tab2" /> + <output name="log"> + <assert_contents> + <has_text text="Expression Results are written" /> + </assert_contents> + </output> + </test> + <test> + <param name="select_job" value="index"/> + <param name="ref_type" value="genomic"/> + <param name="reference_fasta_file" value="ref.fasta" ftype="fasta"/> + <param name="gtf" value="ref.gtf" ftype="gtf"/> + <param name="reference_name" value="ref"/> + <param name="select" value="Yes"/> + <param name="sample" value="rsem_sample"/> + <param name="refSrc" value="self"/> + <param name="format" value="fastq"/> + <param name="matepair" value="single"/> + <param name="singlefastq" value="test.fastq.gz" ftype="fastqsanger.gz"/> + <param name="result_bams" value="none"/> + <output name="reference_file"> + <assert_contents> + <has_text text="ref" /> + </assert_contents> + </output> + <output name="gene_abundances" value="gene_abundances.tab2"/> + <output name="isoform_abundances" value="isoform_abundances.tab2" /> + <output name="log"> + <assert_contents> + <has_text text="Expression Results are written" /> + </assert_contents> + </output> + </test> + <test> + <param name="select_job" value="index"/> + <param name="ref_type" value="genomic"/> + <param name="reference_fasta_file" value="ref.fasta" ftype="fasta"/> + <param name="gtf" value="ref.gtf" ftype="gtf"/> + <param name="reference_name" value="ref"/> + <param name="select" value="No"/> + <output name="reference_file"> + <assert_contents> + <has_text text="ref" /> + </assert_contents> + </output> + </test> + <test> + <param name="select_job" value="index"/> + <param name="ref_type" value="genomic"/> + <param name="reference_fasta_file" value="ref.fasta" ftype="fasta"/> + <param name="gtf" value="ref.gtf" ftype="gtf"/> + <param name="reference_name" value="ref"/> + <param name="select" value="No"/> + <output name="reference_file"> + <assert_contents> + <has_text text="ref" /> + </assert_contents> + </output> + </test> + </tests> + + <help> + +RSEM HOME PAGE - http://deweylab.biostat.wisc.edu/rsem/ + +NAME + rsem-prepare-reference + +SYNOPSIS + rsem-prepare-reference [options] reference_fasta_file(s) reference_name + +DESCRIPTION + The rsem-prepare-reference program extracts/preprocesses the reference sequences and builds Bowtie indices using default parameters. + This program is used in conjunction with the 'rsem-calculate-expression' program. + +INPUTS + A fasta file of transcripts + or + A genome sequence fasta file and a GTF gene annotation file. (When using UCSC data, include the related knownIsoforms.txt) + +--- + +NAME + rsem-calculate-expression - Estimate gene and isoform expression from + RNA-Seq data. + +SYNOPSIS + rsem-calculate-expression [options] upstream_read_file(s) reference_name sample_name + rsem-calculate-expression [options] --paired-end upstream_read_file(s) downstream_read_file(s) reference_name sample_name + rsem-calculate-expression [options] --alignments [--paired-end] input reference_name sample_name + +ARGUMENTS + upstream_read_files(s) + Comma-separated list of files containing single-end reads or + upstream reads for paired-end data. By default, these files are + assumed to be in FASTQ format. If the --no-qualities option is + specified, then FASTA format is expected. + + downstream_read_file(s) + Comma-separated list of files containing downstream reads which are + paired with the upstream reads. By default, these files are assumed + to be in FASTQ format. If the --no-qualities option is specified, + then FASTA format is expected. + + input + SAM/BAM/CRAM formatted input file. If "-" is specified for the + filename, the input is instead assumed to come from standard input. + RSEM requires all alignments of the same read group together. For + paired-end reads, RSEM also requires the two mates of any alignment + be adjacent. In addition, RSEM does not allow the SEQ and QUAL + fields to be empty. See Description section for how to make input + file obey RSEM's requirements. + + reference_name + The name of the reference used. The user must have run + 'rsem-prepare-reference' with this reference_name before running + this program. + + sample_name + The name of the sample analyzed. All output files are prefixed by + this name (e.g., sample_name.genes.results) + +BASIC OPTIONS + --paired-end + Input reads are paired-end reads. (Default: off) + + --no-qualities + Input reads do not contain quality scores. (Default: off) + + --strandedness <none|forward|reverse> + This option defines the strandedness of the RNA-Seq reads. It + recognizes three values: 'none', 'forward', and 'reverse'. 'none' + refers to non-strand-specific protocols. 'forward' means all + (upstream) reads are derived from the forward strand. 'reverse' + means all (upstream) reads are derived from the reverse strand. If + 'forward'/'reverse' is set, the '--norc'/'--nofw' Bowtie/Bowtie 2 + option will also be enabled to avoid aligning reads to the opposite + strand. For Illumina TruSeq Stranded protocols, please use + 'reverse'. (Default: 'none') + + -p/--num-threads <int> + Number of threads to use. Both Bowtie/Bowtie2, expression estimation + and 'samtools sort' will use this many threads. (Default: 1) + + --alignments + Input file contains alignments in SAM/BAM/CRAM format. The exact + file format will be determined automatically. (Default: off) + + --fai <file> + If the header section of input alignment file does not contain + reference sequence information, this option should be turned on. + <file> is a FAI format file containing each reference sequence's + name and length. Please refer to the SAM official website for the + details of FAI format. (Default: off) + + --bowtie2 + Use Bowtie 2 instead of Bowtie to align reads. Since currently RSEM + does not handle indel, local and discordant alignments, the Bowtie2 + parameters are set in a way to avoid those alignments. In + particular, we use options '--sensitive --dpad 0 --gbar 99999999 + --mp 1,1 --np 1 --score-min L,0,-0.1' by default. The last parameter + of '--score-min', '-0.1', is the negative of maximum mismatch rate. + This rate can be set by option '--bowtie2-mismatch-rate'. If reads + are paired-end, we additionally use options '--no-mixed' and + '--no-discordant'. (Default: off) + + --star + Use STAR to align reads. Alignment parameters are from ENCODE3's + STAR-RSEM pipeline. To save computational time and memory resources, + STAR's Output BAM file is unsorted. It is stored in RSEM's temporary + directory with name as 'sample_name.bam'. Each STAR job will have + its own private copy of the genome in memory. (Default: off) + + --append-names + If gene_name/transcript_name is available, append it to the end of + gene_id/transcript_id (separated by '_') in files + 'sample_name.isoforms.results' and 'sample_name.genes.results'. + (Default: off) + + --seed <uint32> + Set the seed for the random number generators used in calculating + posterior mean estimates and credibility intervals. The seed must be + a non-negative 32 bit integer. (Default: off) + + --single-cell-prior + By default, RSEM uses Dirichlet(1) as the prior to calculate + posterior mean estimates and credibility intervals. However, much + less genes are expressed in single cell RNA-Seq data. Thus, if you + want to compute posterior mean estimates and/or credibility + intervals and you have single-cell RNA-Seq data, you are recommended + to turn on this option. Then RSEM will use Dirichlet(0.1) as the + prior which encourage the sparsity of the expression levels. + (Default: off) + + --calc-pme + Run RSEM's collapsed Gibbs sampler to calculate posterior mean + estimates. (Default: off) + + --calc-ci + Calculate 95% credibility intervals and posterior mean estimates. + The credibility level can be changed by setting + '--ci-credibility-level'. (Default: off) + + -q/--quiet + Suppress the output of logging information. (Default: off) + + -h/--help + Show help information. + + --version + Show version information. + +OUTPUT OPTIONS + --sort-bam-by-read-name + Sort BAM file aligned under transcript coordidate by read name. + Setting this option on will produce deterministic maximum likelihood + estimations from independent runs. Note that sorting will take long + time and lots of memory. (Default: off) + + --no-bam-output + Do not output any BAM file. (Default: off) + + --sampling-for-bam + When RSEM generates a BAM file, instead of outputting all alignments + a read has with their posterior probabilities, one alignment is + sampled according to the posterior probabilities. The sampling + procedure includes the alignment to the "noise" transcript, which + does not appear in the BAM file. Only the sampled alignment has a + weight of 1. All other alignments have weight 0. If the "noise" + transcript is sampled, all alignments appeared in the BAM file + should have weight 0. (Default: off) + + --output-genome-bam + Generate a BAM file, 'sample_name.genome.bam', with alignments + mapped to genomic coordinates and annotated with their posterior + probabilities. In addition, RSEM will call samtools (included in + RSEM package) to sort and index the bam file. + 'sample_name.genome.sorted.bam' and + 'sample_name.genome.sorted.bam.bai' will be generated. (Default: + off) + + --sort-bam-by-coordinate + Sort RSEM generated transcript and genome BAM files by coordinates + and build associated indices. (Default: off) + + --sort-bam-memory-per-thread <string> + Set the maximum memory per thread that can be used by 'samtools + sort'. <string> represents the memory and accepts suffices 'K/M/G'. + RSEM will pass <string> to the '-m' option of 'samtools sort'. Note + that the default used here is different from the default used by + samtools. (Default: 1G) + +ALIGNER OPTIONS + --seed-length <int> + Seed length used by the read aligner. Providing the correct value is + important for RSEM. If RSEM runs Bowtie, it uses this value for + Bowtie's seed length parameter. Any read with its or at least one of + its mates' (for paired-end reads) length less than this value will + be ignored. If the references are not added poly(A) tails, the + minimum allowed value is 5, otherwise, the minimum allowed value is + 25. Note that this script will only check if the value >= 5 and give + a warning message if the value < 25 but >= 5. (Default: 25) + + --phred33-quals + Input quality scores are encoded as Phred+33. (Default: on) + + --phred64-quals + Input quality scores are encoded as Phred+64 (default for GA + Pipeline ver. >= 1.3). (Default: off) + + --solexa-quals + Input quality scores are solexa encoded (from GA Pipeline ver. < + 1.3). (Default: off) + + --bowtie-path <path> + The path to the Bowtie executables. (Default: the path to the Bowtie + executables is assumed to be in the user's PATH environment + variable) + + --bowtie-n <int> + (Bowtie parameter) max # of mismatches in the seed. (Range: 0-3, + Default: 2) + + --bowtie-e <int> + (Bowtie parameter) max sum of mismatch quality scores across the + alignment. (Default: 99999999) + + --bowtie-m <int> + (Bowtie parameter) suppress all alignments for a read if > <int> + valid alignments exist. (Default: 200) + + --bowtie-chunkmbs <int> + (Bowtie parameter) memory allocated for best first alignment + calculation (Default: 0 - use Bowtie's default) + + --bowtie2-path <path> + (Bowtie 2 parameter) The path to the Bowtie 2 executables. (Default: + the path to the Bowtie 2 executables is assumed to be in the user's + PATH environment variable) + + --bowtie2-mismatch-rate <double> + (Bowtie 2 parameter) The maximum mismatch rate allowed. (Default: + 0.1) + + --bowtie2-k <int> + (Bowtie 2 parameter) Find up to <int> alignments per read. (Default: + 200) + + --bowtie2-sensitivity-level <string> + (Bowtie 2 parameter) Set Bowtie 2's preset options in --end-to-end + mode. This option controls how hard Bowtie 2 tries to find + alignments. <string> must be one of "very_fast", "fast", "sensitive" + and "very_sensitive". The four candidates correspond to Bowtie 2's + "--very-fast", "--fast", "--sensitive" and "--very-sensitive" + options. (Default: "sensitive" - use Bowtie 2's default) + + --star-path <path> + The path to STAR's executable. (Default: the path to STAR executable + is assumed to be in user's PATH environment variable) + + --star-gzipped-read-file + (STAR parameter) Input read file(s) is compressed by gzip. (Default: + off) + + --star-bzipped-read-file + (STAR parameter) Input read file(s) is compressed by bzip2. + (Default: off) + + --star-output-genome-bam + (STAR parameter) Save the BAM file from STAR alignment under genomic + coordinate to 'sample_name.STAR.genome.bam'. This file is NOT sorted + by genomic coordinate. In this file, according to STAR's manual, + 'paired ends of an alignment are always adjacent, and multiple + alignments of a read are adjacent as well'. (Default: off) + +ADVANCED OPTIONS + --tag <string> + The name of the optional field used in the SAM input for identifying + a read with too many valid alignments. The field should have the + format <tagName>:i:<value>, where a <value> bigger than 0 indicates + a read with too many alignments. (Default: "") + + --fragment-length-min <int> + Minimum read/insert length allowed. This is also the value for the + Bowtie/Bowtie2 -I option. (Default: 1) + + --fragment-length-max <int> + Maximum read/insert length allowed. This is also the value for the + Bowtie/Bowtie 2 -X option. (Default: 1000) + + --fragment-length-mean <double> + (single-end data only) The mean of the fragment length distribution, + which is assumed to be a Gaussian. (Default: -1, which disables use + of the fragment length distribution) + + --fragment-length-sd <double> + (single-end data only) The standard deviation of the fragment length + distribution, which is assumed to be a Gaussian. (Default: 0, which + assumes that all fragments are of the same length, given by the + rounded value of --fragment-length-mean) + + --estimate-rspd + Set this option if you want to estimate the read start position + distribution (RSPD) from data. Otherwise, RSEM will use a uniform + RSPD. (Default: off) + + --num-rspd-bins <int> + Number of bins in the RSPD. Only relevant when '--estimate-rspd' is + specified. Use of the default setting is recommended. (Default: 20) + + --gibbs-burnin <int> + The number of burn-in rounds for RSEM's Gibbs sampler. Each round + passes over the entire data set once. If RSEM can use multiple + threads, multiple Gibbs samplers will start at the same time and all + samplers share the same burn-in number. (Default: 200) + + --gibbs-number-of-samples <int> + The total number of count vectors RSEM will collect from its Gibbs + samplers. (Default: 1000) + + --gibbs-sampling-gap <int> + The number of rounds between two succinct count vectors RSEM + collects. If the count vector after round N is collected, the count + vector after round N + <int> will also be collected. (Default: 1) + + --ci-credibility-level <double> + The credibility level for credibility intervals. (Default: 0.95) + + --ci-memory <int> + Maximum size (in memory, MB) of the auxiliary buffer used for + computing credibility intervals (CI). (Default: 1024) + + --ci-number-of-samples-per-count-vector <int> + The number of read generating probability vectors sampled per + sampled count vector. The crebility intervals are calculated by + first sampling P(C | D) and then sampling P(Theta | C) for each + sampled count vector. This option controls how many Theta vectors + are sampled per sampled count vector. (Default: 50) + + --keep-intermediate-files + Keep temporary files generated by RSEM. RSEM creates a temporary + directory, 'sample_name.temp', into which it puts all intermediate + output files. If this directory already exists, RSEM overwrites all + files generated by previous RSEM runs inside of it. By default, + after RSEM finishes, the temporary directory is deleted. Set this + option to prevent the deletion of this directory and the + intermediate files inside of it. (Default: off) + + --temporary-folder <string> + Set where to put the temporary files generated by RSEM. If the + folder specified does not exist, RSEM will try to create it. + (Default: sample_name.temp) + + --time + Output time consumed by each step of RSEM to 'sample_name.time'. + (Default: off) + +PRIOR-ENHANCED RSEM OPTIONS + --run-pRSEM + Running prior-enhanced RSEM (pRSEM). Prior parameters, i.e. + isoform's initial pseudo-count for RSEM's Gibbs sampling, will be + learned from input RNA-seq data and an external data set. When pRSEM + needs and only needs ChIP-seq peak information to partition isoforms + (e.g. in pRSEM's default partition model), either ChIP-seq peak file + (with the '--chipseq-peak-file' option) or ChIP-seq FASTQ files for + target and input and the path for Bowtie executables are required + (with the '--chipseq-target-read-files <string>', + '--chipseq-control-read-files <string>', and '--bowtie-path <path> + options), otherwise, ChIP-seq FASTQ files for target and control and + the path to Bowtie executables are required. (Default: off) + + --chipseq-peak-file <string> + Full path to a ChIP-seq peak file in ENCODE's narrowPeak, i.e. + BED6+4, format. This file is used when running prior-enhanced RSEM + in the default two-partition model. It partitions isoforms by + whether they have ChIP-seq overlapping with their transcription + start site region or not. Each partition will have its own prior + parameter learned from a training set. This file can be either + gzipped or ungzipped. (Default: "") + + --chipseq-target-read-files <string> + Comma-separated full path of FASTQ read file(s) for ChIP-seq target. + This option is used when running prior-enhanced RSEM. It provides + information to calculate ChIP-seq peaks and signals. The file(s) can + be either ungzipped or gzipped with a suffix '.gz' or '.gzip'. The + options '--bowtie-path <path>' and '--chipseq-control-read-files + <string>' must be defined when this option is specified. (Default: + "") + + --chipseq-control-read-files <string> + Comma-separated full path of FASTQ read file(s) for ChIP-seq conrol. + This option is used when running prior-enhanced RSEM. It provides + information to call ChIP-seq peaks. The file(s) can be either + ungzipped or gzipped with a suffix '.gz' or '.gzip'. The options + '--bowtie-path <path>' and '--chipseq-target-read-files <string>' + must be defined when this option is specified. (Default: "") + + --chipseq-read-files-multi-targets <string> + Comma-separated full path of FASTQ read files for multiple ChIP-seq + targets. This option is used when running prior-enhanced RSEM, where + prior is learned from multiple complementary data sets. It provides + information to calculate ChIP-seq signals. All files can be either + ungzipped or gzipped with a suffix '.gz' or '.gzip'. When this + option is specified, the option '--bowtie-path <path>' must be + defined and the option '--partition-model <string>' will be set to + 'cmb_lgt' automatically. (Default: "") + + --chipseq-bed-files-multi-targets <string> + Comma-separated full path of BED files for multiple ChIP-seq + targets. This option is used when running prior-enhanced RSEM, where + prior is learned from multiple complementary data sets. It provides + information of ChIP-seq signals and must have at least the first six + BED columns. All files can be either ungzipped or gzipped with a + suffix '.gz' or '.gzip'. When this option is specified, the option + '--partition-model <string>' will be set to 'cmb_lgt' automatically. + (Default: "") + + --cap-stacked-chipseq-reads + Keep a maximum number of ChIP-seq reads that aligned to the same + genomic interval. This option is used when running prior-enhanced + RSEM, where prior is learned from multiple complementary data sets. + This option is only in use when either + '--chipseq-read-files-multi-targets <string>' or + '--chipseq-bed-files-multi-targets <string>' is specified. (Default: + off) + + --n-max-stacked-chipseq-reads <int> + The maximum number of stacked ChIP-seq reads to keep. This option is + used when running prior-enhanced RSEM, where prior is learned from + multiple complementary data sets. This option is only in use when + the option '--cap-stacked-chipseq-reads' is set. (Default: 5) + + --partition-model <string> + A keyword to specify the partition model used by prior-enhanced + RSEM. It must be one of the following keywords: + + - pk + Partitioned by whether an isoform has a ChIP-seq peak overlapping + with its transcription start site (TSS) region. The TSS region is + defined as [TSS-500bp, TSS+500bp]. For simplicity, we refer this + type of peak as 'TSS peak' when explaining other keywords. + + - pk_lgtnopk + First partitioned by TSS peak. Then, for isoforms in the 'no TSS + peak' set, a logistic model is employed to further classify them + into two partitions. + + - lm3, lm4, lm5, or lm6 + Based on their ChIP-seq signals, isoforms are classified into 3, + 4, 5, or 6 partitions by a linear regression model. + + - nopk_lm2pk, nopk_lm3pk, nopk_lm4pk, or nopk_lm5pk + First partitioned by TSS peak. Then, for isoforms in the 'with TSS + peak' set, a linear regression model is employed to further + classify them into 2, 3, 4, or 5 partitions. + + - pk_lm2nopk, pk_lm3nopk, pk_lm4nopk, or pk_lm5nopk + First partitioned by TSS peak. Then, for isoforms in the 'no TSS + peak' set, a linear regression model is employed to further + classify them into 2, 3, 4, or 5 partitions. + + - cmb_lgt + Using a logistic regression to combine TSS signals from multiple + complementary data sets and partition training set isoform into + 'expressed' and 'not expressed'. This partition model is only in + use when either '--chipseq-read-files-multi-targets <string>' or + '--chipseq-bed-files-multi-targets <string> is specified. + + Parameters for all the above models are learned from a training set. + For detailed explanations, please see prior-enhanced RSEM's paper. + (Default: 'pk') + +DEPRECATED OPTIONS + The options in this section are deprecated. They are here only for + compatibility reasons and may be removed in future releases. + + --sam + Inputs are alignments in SAM format. (Default: off) + + --bam + Inputs are alignments in BAM format. (Default: off) + + --strand-specific + Equivalent to '--strandedness forward'. (Default: off) + + --forward-prob <double> + Probability of generating a read from the forward strand of a + transcript. Set to 1 for a strand-specific protocol where all + (upstream) reads are derived from the forward strand, 0 for a + strand-specific protocol where all (upstream) read are derived from + the reverse strand, or 0.5 for a non-strand-specific protocol. + (Default: off) + +DESCRIPTION + In its default mode, this program aligns input reads against a reference + transcriptome with Bowtie and calculates expression values using the + alignments. RSEM assumes the data are single-end reads with quality + scores, unless the '--paired-end' or '--no-qualities' options are + specified. Alternatively, users can use STAR to align reads using the + '--star' option. RSEM has provided options in 'rsem-prepare-reference' + to prepare STAR's genome indices. Users may use an alternative aligner + by specifying '--alignments', and providing an alignment file in + SAM/BAM/CRAM format. However, users should make sure that they align + against the indices generated by 'rsem-prepare-reference' and the + alignment file satisfies the requirements mentioned in ARGUMENTS + section. + + One simple way to make the alignment file satisfying RSEM's requirements + is to use the 'convert-sam-for-rsem' script. This script accepts + SAM/BAM/CRAM files as input and outputs a BAM file. For example, type + the following command to convert a SAM file, 'input.sam', to a + ready-for-use BAM file, 'input_for_rsem.bam': + + convert-sam-for-rsem input.sam input_for_rsem + + For details, please refer to 'convert-sam-for-rsem's documentation page. + +NOTES + 1. Users must run 'rsem-prepare-reference' with the appropriate + reference before using this program. + + 2. For single-end data, it is strongly recommended that the user provide + the fragment length distribution parameters (--fragment-length-mean and + --fragment-length-sd). For paired-end data, RSEM will automatically + learn a fragment length distribution from the data. + + 3. Some aligner parameters have default values different from their + original settings. + + 4. With the '--calc-pme' option, posterior mean estimates will be + calculated in addition to maximum likelihood estimates. + + 5. With the '--calc-ci' option, 95% credibility intervals and posterior + mean estimates will be calculated in addition to maximum likelihood + estimates. + + 6. The temporary directory and all intermediate files will be removed + when RSEM finishes unless '--keep-intermediate-files' is specified. + + With the '--run-pRSEM' option and associated options (see section + 'PRIOR-ENHANCED RSEM OPTIONS' above for details), prior-enhanced RSEM + will be running. Prior parameters will be learned from supplied external + data set(s) and assigned as initial pseudo-counts for isoforms in the + corresponding partition for Gibbs sampling. + +OUTPUT + sample_name.isoforms.results + File containing isoform level expression estimates. The first line + contains column names separated by the tab character. The format of + each line in the rest of this file is: + + transcript_id gene_id length effective_length expected_count TPM + FPKM IsoPct [posterior_mean_count + posterior_standard_deviation_of_count pme_TPM pme_FPKM + IsoPct_from_pme_TPM TPM_ci_lower_bound TPM_ci_upper_bound + TPM_coefficient_of_quartile_variation FPKM_ci_lower_bound + FPKM_ci_upper_bound FPKM_coefficient_of_quartile_variation] + + Fields are separated by the tab character. Fields within "[]" are + optional. They will not be presented if neither '--calc-pme' nor + '--calc-ci' is set. + + 'transcript_id' is the transcript name of this transcript. 'gene_id' + is the gene name of the gene which this transcript belongs to + (denote this gene as its parent gene). If no gene information is + provided, 'gene_id' and 'transcript_id' are the same. + + 'length' is this transcript's sequence length (poly(A) tail is not + counted). 'effective_length' counts only the positions that can + generate a valid fragment. If no poly(A) tail is added, + 'effective_length' is equal to transcript length - mean fragment + length + 1. If one transcript's effective length is less than 1, + this transcript's both effective length and abundance estimates are + set to 0. + + 'expected_count' is the sum of the posterior probability of each + read comes from this transcript over all reads. Because 1) each read + aligning to this transcript has a probability of being generated + from background noise; 2) RSEM may filter some alignable low quality + reads, the sum of expected counts for all transcript are generally + less than the total number of reads aligned. + + 'TPM' stands for Transcripts Per Million. It is a relative measure + of transcript abundance. The sum of all transcripts' TPM is 1 + million. 'FPKM' stands for Fragments Per Kilobase of transcript per + Million mapped reads. It is another relative measure of transcript + abundance. If we define l_bar be the mean transcript length in a + sample, which can be calculated as + + l_bar = \sum_i TPM_i / 10^6 * effective_length_i (i goes through + every transcript), + + the following equation is hold: + + FPKM_i = 10^3 / l_bar * TPM_i. + + We can see that the sum of FPKM is not a constant across samples. + + 'IsoPct' stands for isoform percentage. It is the percentage of this + transcript's abandunce over its parent gene's abandunce. If its + parent gene has only one isoform or the gene information is not + provided, this field will be set to 100. + + 'posterior_mean_count', 'pme_TPM', 'pme_FPKM' are posterior mean + estimates calculated by RSEM's Gibbs sampler. + 'posterior_standard_deviation_of_count' is the posterior standard + deviation of counts. 'IsoPct_from_pme_TPM' is the isoform percentage + calculated from 'pme_TPM' values. + + 'TPM_ci_lower_bound', 'TPM_ci_upper_bound', 'FPKM_ci_lower_bound' + and 'FPKM_ci_upper_bound' are lower(l) and upper(u) bounds of 95% + credibility intervals for TPM and FPKM values. The bounds are + inclusive (i.e. [l, u]). + + 'TPM_coefficient_of_quartile_variation' and + 'FPKM_coefficient_of_quartile_variation' are coefficients of + quartile variation (CQV) for TPM and FPKM values. CQV is a robust + way of measuring the ratio between the standard deviation and the + mean. It is defined as + + CQV := (Q3 - Q1) / (Q3 + Q1), + + where Q1 and Q3 are the first and third quartiles. + + sample_name.genes.results + File containing gene level expression estimates. The first line + contains column names separated by the tab character. The format of + each line in the rest of this file is: + + gene_id transcript_id(s) length effective_length expected_count TPM + FPKM [posterior_mean_count posterior_standard_deviation_of_count + pme_TPM pme_FPKM TPM_ci_lower_bound TPM_ci_upper_bound + TPM_coefficient_of_quartile_variation FPKM_ci_lower_bound + FPKM_ci_upper_bound FPKM_coefficient_of_quartile_variation] + + Fields are separated by the tab character. Fields within "[]" are + optional. They will not be presented if neither '--calc-pme' nor + '--calc-ci' is set. + + 'transcript_id(s)' is a comma-separated list of transcript_ids + belonging to this gene. If no gene information is provided, + 'gene_id' and 'transcript_id(s)' are identical (the + 'transcript_id'). + + A gene's 'length' and 'effective_length' are defined as the weighted + average of its transcripts' lengths and effective lengths (weighted + by 'IsoPct'). A gene's abundance estimates are just the sum of its + transcripts' abundance estimates. + + sample_name.alleles.results + Only generated when the RSEM references are built with + allele-specific transcripts. + + This file contains allele level expression estimates for + allele-specific expression calculation. The first line contains + column names separated by the tab character. The format of each line + in the rest of this file is: + + allele_id transcript_id gene_id length effective_length + expected_count TPM FPKM AlleleIsoPct AlleleGenePct + [posterior_mean_count posterior_standard_deviation_of_count pme_TPM + pme_FPKM AlleleIsoPct_from_pme_TPM AlleleGenePct_from_pme_TPM + TPM_ci_lower_bound TPM_ci_upper_bound + TPM_coefficient_of_quartile_variation FPKM_ci_lower_bound + FPKM_ci_upper_bound FPKM_coefficient_of_quartile_variation] + + Fields are separated by the tab character. Fields within "[]" are + optional. They will not be presented if neither '--calc-pme' nor + '--calc-ci' is set. + + 'allele_id' is the allele-specific name of this allele-specific + transcript. + + 'AlleleIsoPct' stands for allele-specific percentage on isoform + level. It is the percentage of this allele-specific transcript's + abundance over its parent transcript's abundance. If its parent + transcript has only one allele variant form, this field will be set + to 100. + + 'AlleleGenePct' stands for allele-specific percentage on gene level. + It is the percentage of this allele-specific transcript's abundance + over its parent gene's abundance. + + 'AlleleIsoPct_from_pme_TPM' and 'AlleleGenePct_from_pme_TPM' have + similar meanings. They are calculated based on posterior mean + estimates. + + Please note that if this file is present, the fields 'length' and + 'effective_length' in 'sample_name.isoforms.results' should be + interpreted similarly as the corresponding definitions in + 'sample_name.genes.results'. + + sample_name.transcript.bam + Only generated when --no-bam-output is not specified. + + 'sample_name.transcript.bam' is a BAM-formatted file of read + alignments in transcript coordinates. The MAPQ field of each + alignment is set to min(100, floor(-10 * log10(1.0 - w) + 0.5)), + where w is the posterior probability of that alignment being the + true mapping of a read. In addition, RSEM pads a new tag ZW:f:value, + where value is a single precision floating number representing the + posterior probability. Because this file contains all alignment + lines produced by bowtie or user-specified aligners, it can also be + used as a replacement of the aligner generated BAM/SAM file. + + sample_name.transcript.sorted.bam and sample_name.transcript.sorted.bam.bai + Only generated when --no-bam-output is not specified and --sort-bam-by-coordinate is specified. + + 'sample_name.transcript.sorted.bam' and + 'sample_name.transcript.sorted.bam.bai' are the sorted BAM file and + indices generated by samtools (included in RSEM package). + + sample_name.genome.bam + Only generated when --no-bam-output is not specified and + --output-genome-bam is specified. + + 'sample_name.genome.bam' is a BAM-formatted file of read alignments + in genomic coordinates. Alignments of reads that have identical + genomic coordinates (i.e., alignments to different isoforms that + share the same genomic region) are collapsed into one alignment. The + MAPQ field of each alignment is set to min(100, floor(-10 * + log10(1.0 - w) + 0.5)), where w is the posterior probability of that + alignment being the true mapping of a read. In addition, RSEM pads a + new tag ZW:f:value, where value is a single precision floating + number representing the posterior probability. If an alignment is + spliced, a XS:A:value tag is also added, where value is either '+' + or '-' indicating the strand of the transcript it aligns to. + + sample_name.genome.sorted.bam and sample_name.genome.sorted.bam.bai + Only generated when --no-bam-output is not specified, and + --sort-bam-by-coordinate and --output-genome-bam are specified. + + 'sample_name.genome.sorted.bam' and + 'sample_name.genome.sorted.bam.bai' are the sorted BAM file and + indices generated by samtools (included in RSEM package). + + sample_name.time + Only generated when --time is specified. + + It contains time (in seconds) consumed by aligning reads, estimating + expression levels and calculating credibility intervals. + + sample_name.stat + This is a folder instead of a file. All model related statistics are + stored in this folder. Use 'rsem-plot-model' can generate plots + using this folder. + + 'sample_name.stat/sample_name.cnt' contains alignment statistics. + The format and meanings of each field are described in + 'cnt_file_description.txt' under RSEM directory. + + 'sample_name.stat/sample_name.model' stores RNA-Seq model parameters + learned from the data. The format and meanings of each filed of this + file are described in 'model_file_description.txt' under RSEM + directory. + + The following four output files will be generated only by + prior-enhanced RSEM + + - 'sample_name.stat/sample_name_prsem.all_tr_features' + It stores isofrom features for deriving and assigning pRSEM prior. + The first line is a header and the rest is one isoform per line. + The description for each column is: + + * trid: transcript ID from input annotation + + * geneid: gene ID from input anntation + + * chrom: isoform's chromosome name + + * strand: isoform's strand name + + * start: isoform's end with the lowest genomic loci + + * end: isoform's end with the highest genomic loci + + * tss_mpp: average mappability of [TSS-500bp, TSS+500bp], where + TSS is isoform's transcription start site, i.e. 5'-end + + * body_mpp: average mappability of (TSS+500bp, TES-500bp), where + TES is isoform's transcription end site, i.e. 3'-end + + * tes_mpp: average mappability of [TES-500bp, TES+500bp] + + * pme_count: isoform's fragment or read count from RSEM's + posterior mean estimates + + * tss: isoform's TSS loci + + * tss_pk: equal to 1 if isoform's [TSS-500bp, TSS+500bp] region + overlaps with a RNA Pol II peak; 0 otherwise + + * is_training: equal to 1 if isoform is in the training set where + Pol II prior is learned; 0 otherwise + + - 'sample_name.stat/sample_name_prsem.all_tr_prior' + It stores prior parameters for every isoform. This file does not + have a header. Each line contains a prior parameter and an + isoform's transcript ID delimited by " # ". + + - 'sample_name.stat/sample_name_uniform_prior_1.isoforms.results' + RSEM's posterior mean estimates on the isoform level with an + initial pseudo-count of one for every isoform. It is in the same + format as the 'sample_name.isoforms.results'. + + - 'sample_name.stat/sample_name_uniform_prior_1.genes.results' + RSEM's posterior mean estimates on the gene level with an initial + pseudo-count of one for every isoform. It is in the same format as + the 'sample_name.genes.results'. + + When learning prior from multiple external data sets in + prior-enhanced RSEM, two additional output files will be generated. + + - 'sample_name.stat/sample_name.pval_LL' + It stores a p-value and a log-likelihood. The p-value indicates + whether the combination of multiple complementary data sets is + informative for RNA-seq quantification. The log-likelihood shows + how well pRSEM's Dirichlet-multinomial model fits the read counts + of partitioned training set isoforms. + + - 'sample_name.stat/sample_name.lgt_mdl.RData' + It stores an R object named 'glmmdl', which is a logistic + regression model on the training set isoforms and multiple + external data sets. + + In addition, extra columns will be added to + 'sample_name.stat/all_tr_features' + + * is_expr: equal to 1 if isoform has an abundance >= 1 TPM and a + non-zero read count from RSEM's posterior mean estimates; 0 + otherwise + + * "$external_data_set_basename": log10 of external data's signal at + [TSS-500, TSS+500]. Signal is the number of reads aligned within + that interval and normalized to RPKM by read depth and interval + length. It will be set to -4 if no read aligned to that interval. + + There are multiple columns like this one, where each represents an + external data set. + + * prd_expr_prob: predicted probability from logistic regression + model on whether this isoform is expressed or not. A probability + higher than 0.5 is considered as expressed + + * partition: group index, to which this isoforms is partitioned + + * prior: prior parameter for this isoform + +EXAMPLES + Assume the path to the bowtie executables is in the user's PATH + environment variable. Reference files are under '/ref' with name + 'mouse_125'. + + 1) '/data/mmliver.fq', single-end reads with quality scores. Quality + scores are encoded as for 'GA pipeline version >= 1.3'. We want to use 8 + threads and generate a genome BAM file. In addition, we want to append + gene/transcript names to the result files: + + rsem-calculate-expression --phred64-quals \ + -p 8 \ + --append-names \ + --output-genome-bam \ + /data/mmliver.fq \ + /ref/mouse_125 \ + mmliver_single_quals + + 2) '/data/mmliver_1.fq' and '/data/mmliver_2.fq', stranded paired-end + reads with quality scores. Suppose the library is prepared using TruSeq + Stranded Kit, which means the first mate should map to the reverse + strand. Quality scores are in SANGER format. We want to use 8 threads + and do not generate a genome BAM file: + + rsem-calculate-expression -p 8 \ + --paired-end \ + --strandedness reverse \ + /data/mmliver_1.fq \ + /data/mmliver_2.fq \ + /ref/mouse_125 \ + mmliver_paired_end_quals + + 3) '/data/mmliver.fa', single-end reads without quality scores. We want + to use 8 threads: + + rsem-calculate-expression -p 8 \ + --no-qualities \ + /data/mmliver.fa \ + /ref/mouse_125 \ + mmliver_single_without_quals + + 4) Data are the same as 1). This time we assume the bowtie executables + are under '/sw/bowtie'. We want to take a fragment length distribution + into consideration. We set the fragment length mean to 150 and the + standard deviation to 35. In addition to a BAM file, we also want to + generate credibility intervals. We allow RSEM to use 1GB of memory for + CI calculation: + + rsem-calculate-expression --bowtie-path /sw/bowtie \ + --phred64-quals \ + --fragment-length-mean 150.0 \ + --fragment-length-sd 35.0 \ + -p 8 \ + --output-genome-bam \ + --calc-ci \ + --ci-memory 1024 \ + /data/mmliver.fq \ + /ref/mouse_125 \ + mmliver_single_quals + + 5) '/data/mmliver_paired_end_quals.bam', BAM-formatted alignments for + paired-end reads with quality scores. We want to use 8 threads: + + rsem-calculate-expression --paired-end \ + --alignments \ + -p 8 \ + /data/mmliver_paired_end_quals.bam \ + /ref/mouse_125 \ + mmliver_paired_end_quals + + 6) '/data/mmliver_1.fq.gz' and '/data/mmliver_2.fq.gz', paired-end reads + with quality scores and read files are compressed by gzip. We want to + use STAR to aligned reads and assume STAR executable is '/sw/STAR'. + Suppose we want to use 8 threads and do not generate a genome BAM file: + + rsem-calculate-expression --paired-end \ + --star \ + --star-path /sw/STAR \ + --gzipped-read-file \ + --paired-end \ + -p 8 \ + /data/mmliver_1.fq.gz \ + /data/mmliver_2.fq.gz \ + /ref/mouse_125 \ + mmliver_paired_end_quals + + 7) In the above example, suppose we want to run prior-enhanced RSEM + instead. Assuming we want to learn priors from a ChIP-seq peak file + '/data/mmlive.narrowPeak.gz': + + rsem-calculate-expression --star \ + --star-path /sw/STAR \ + --gzipped-read-file \ + --paired-end \ + --calc-pme \ + --run-pRSEM \ + --chipseq-peak-file /data/mmliver.narrowPeak.gz \ + -p 8 \ + /data/mmliver_1.fq.gz \ + /data/mmliver_2.fq.gz \ + /ref/mouse_125 \ + mmliver_paired_end_quals + + 8) Similar to the example in 7), suppose we want to use the partition + model 'pk_lm2nopk' (partitioning isoforms by Pol II TSS peak first and + then partitioning 'no TSS peak' isoforms into two bins by a linear + regression model), and we want to partition isoforms by RNA Pol II's + ChIP-seq read files '/data/mmliver_PolIIRep1.fq.gz' and + '/data/mmliver_PolIIRep2.fq.gz', and the control ChIP-seq read files + '/data/mmliver_ChIPseqCtrl.fq.gz'. Also, assuming Bowtie's executables + are under '/sw/bowtie/': + + rsem-calculate-expression --star \ + --star-path /sw/STAR \ + --gzipped-read-file \ + --paired-end \ + --calc-pme \ + --run-pRSEM \ + --chipseq-target-read-files /data/mmliver_PolIIRep1.fq.gz,/data/mmliver_PolIIRep2.fq.gz \ + --chipseq-control-read-files /data/mmliver_ChIPseqCtrl.fq.gz \ + --partition-model pk_lm2nopk \ + --bowtie-path /sw/bowtie \ + -p 8 \ + /data/mmliver_1.fq.gz \ + /data/mmliver_2.fq.gz \ + /ref/mouse_125 \ + mmliver_paired_end_quals + + 9) Similar to the example in 8), suppose we want to derive prior from + four histone modification ChIP-seq read data sets: + '/data/H3K27Ac.fastq.gz', '/data/H3K4me1.fastq.gz', + '/data/H3K4me2.fastq.gz', and '/data/H3K4me3.fastq.gz'. Also, assuming + Bowtie's executables are under '/sw/bowtie/': + + rsem-calculate-expression --star \ + --star-path /sw/STAR \ + --gzipped-read-file \ + --paired-end \ + --calc-pme \ + --run-pRSEM \ + --partition-model cmb_lgt \ + --chipseq-read-files-multi-targets /data/H3K27Ac.fastq.gz,/data/H3K4me1.fastq.gz,/data/H3K4me2.fastq.gz,/data/H3K4me3.fastq.gz \ + --bowtie-path /sw/bowtie \ + -p 8 \ + /data/mmliver_1.fq.gz \ + /data/mmliver_2.fq.gz \ + /ref/mouse_125 \ + mmliver_paired_end_quals + + </help> + <citations> + <citation type="doi">10.1186/1471-2105-12-323</citation> + </citations> + +</tool>
--- a/rsem.xml Wed Mar 28 10:43:28 2018 -0400 +++ b/rsem.xml Sat Mar 31 10:17:42 2018 -0400 @@ -1,4 +1,4 @@ -<tool id="rsem" name="RSEM" version="0.3.1"> +<tool id="rsembowtie" name="RSEM-Bowtie" version="0.4.0"> <description></description> <macros> <import>macros.xml</import> @@ -47,6 +47,7 @@ #end if #if $run_rsem.select == "Yes": + ## uncompress fastq.gz or fastqsanger.gz if needed #if $run_rsem.input.fastq.matepair=="single": #if $run_rsem.input.fastq.singlefastq.is_of_type('fastq.gz') or $run_rsem.input.fastq.singlefastq.is_of_type('fastqsanger.gz'): gunzip < '$run_rsem.input.fastq.singlefastq' > uncomp_single.fastq && @@ -121,6 +122,8 @@ ## Outputs #if $run_rsem.rsem_outputs.result_bams == 'none': --no-bam-output + #elif $run_rsem.rsem_outputs.result_bams == 'default': + --sort-bam-by-coordinate #else --sort-bam-by-coordinate --output-genome-bam @@ -335,12 +338,10 @@ <filter>run_rsem['select'] == "Yes"</filter> </data> <data format="bam" name="transcript_sorted_bam" label="${run_rsem.sample}.transcript.bam" from_work_dir="rsem_output.transcript.sorted.bam" > - <filter>run_rsem['select'] == "Yes"</filter> - <filter>rsem_outputs['result_bams'] != "none"</filter> + <filter>run_rsem['select'] == "Yes" and run_rsem['rsem_outputs']['result_bams'] != "none"</filter> </data> <data format="bam" name="genome_sorted_bam" label="${run_rsem.sample}.genome.bam" from_work_dir="rsem_output.genome.sorted.bam"> - <filter>run_rsem['select'] == "Yes"</filter> - <filter>rsem_outputs['result_bams'] == "both"</filter> + <filter>run_rsem['select'] == "Yes" and run_rsem['rsem_outputs']['result_bams'] == "both"</filter> </data> <data format="txt" name="log" label="${run_rsem.sample}.rsem_log"> <filter>run_rsem['select'] == "Yes"</filter>
--- a/test-data/bowtie-alignment.tab Wed Mar 28 10:43:28 2018 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,1109 +0,0 @@ -AMELIA:165:C03TUABXX:5:1101:20072:31903 - 1 14721 CCTCTGCCTGTGGCTGCTGCGGTGGCGGCAGAGGAGGGATGGAGTCTGACACGCGGGCAAAGGCTCCTCCGGGCCCCTCACCAGCCCCAGGTCCTTTCCCA @BBDDDDBDDDDDDDDDDDDDDDDDDDCCCDDDDDDDDDDDDDDDDCB=@@DFFFHHHHHHJJIIJIIIIJIJJJJJIHFJJJJJJJJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:12097:28848 - 1 16908 GCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAGGTCGGCAATGTACATGAGGTCGTTGGCAATGCCGGGCAGGTCAGGCAGGTAGGATGGAACATCAATCT BB?888>::C99)0@@@>CBB9088,,(((3>3D=HFFHHCHD=4DEC@<BA7GGGIEEHD@:@F@D>GIIIHEHC>GDHFHH<HGGGAF?AFD>BDD@?@ 0 -AMELIA:165:C03TUABXX:5:1101:13000:2187 + 1 17262 GGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCTGCCCCCAGCATCACATGGGTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCT CCCFFFFFHHHHGJJJJJJJJJJJJJJJIGJIIJJJJJJIJJIIIJIJJJJIJJHHHGFFFFEFCEEEECDDDBD@ABDDDDDDDDDDDDDDDDDCDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:21256:22282 + 1 18287 CCTTGATCTTCTCAATCTTGGCCTGGGCCAAGGAGACCTTCTCTCCAATGGCCTGCACCTGGCTCCGGCTCTGCTCTACCCGCTGGGAGATCCTGCTGAAG ?=@A=DDDA<,ACGIEFDHHFFGG7+<8ABGGBE3?F19DB?DFDAEI98BDF97CBFE>>6@EE@884='.(;@>ABA(58;B################# 0 80:T>C,96:C>T,97:A>G,98:T>A,99:G>A -AMELIA:165:C03TUABXX:5:1101:19155:32547 - 1 24737 CTGCTGAAGATGTCTCCAGAGACCTTCTGCAGGTACTGCAGGGCATCCGCCATCTGCTGGACGGCCTCCTCTCGCCGCAGGTCTGGCTGGATGAAGGGCAC <@ACDDCCCC?@C?>:ACC>@9?CC@AC<4:CCACCCBBCC@B:-@?BCCC@CECCECE?:EC@;@>IHGHHAFDCE@GHGIIHGBEGHHFBBBDDDD<?? 0 -AMELIA:165:C03TUABXX:5:1101:18778:29474 + 1 526987 CCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTGCAGTGAGCCGAGATCCCGCCACTGCACTCCAGCCT C@@FDFEEHHHHHJIIJJIJJJHHIIJIGHIGGHJGIDIEIGGGDHIGGIIGIFBEDDBDBBBACDBCCDDAACD@BD<BCDDDDDBBCDDCDDDDDDDBC 0 -AMELIA:165:C03TUABXX:5:1101:10989:33205 - 1 710921 TAGAATTAGAGACATCTGGGCCAGGCACAGTGGCTGACGCCTGTAATCCTAACACTTTGGGAAGCCGAGGCAGGCAGATCACCTAAGGTCAGGAGTTCGAG EEEEEDEDDEDEDDDDDDDDDEEEEEEEFFFFHHHHJJJJJJJIJJIJIJIIIHJJJJJJJJJJJJJIJJJJJJJJJJJIHJJJJJJJHHHHHFFFFFCCC 1 -AMELIA:165:C03TUABXX:5:1101:11390:26163 + 1 330400 CTCTGCCCCCCGGGTGGCTCAGCCCAGCTCCTGCCTAGGAAAGCCTTAGTGTTGGGAGGGACCCTGATGACTGAGGAGCCTGGTAGCTCCAGGTCGCCCAC CCCFFFFFHHHHHJDHIJJJJJIJJJJJJJJGJIJJJJIJIIHHHHHFFCEDFDDDDDDD@@BBDDDDDDDDDDDDCDDDDDDCDDCCDDDDDCDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:9102:10322 - 1 660381 GTTGAAAAAAAACTCGGGAAAAAAAAAAAAGGGGGCAGCTTTTAACCAAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGACGGAGTCTCGCTCTGTCG ####################################################DDDDDDDDDDDDDDDFJJJJJJJJJJJJJJHJJJJJHHHHGFFFFFCCC 0 9:T>C,16:C>G,50:T>C,51:C>G,52:T>A,53:C>A,55:T>C,56:T>A,57:C>A,58:C>T,59:G>T,60:G>T,61:A>T,62:A>C,63:T>G,64:G>A,65:A>C,68:C>G,69:A>G,71:G>A,72:G>A,73:T>A,74:T>A,75:T>A,76:G>A,78:C>A,79:T>A,80:T>A,81:T>A,82:C>A,83:C>G,84:T>G,85:A>G,86:A>C,88:T>C,89:T>A,91:C>A,92:C>A,93:G>A,96:G>A,97:T>G,98:G>T,99:G>T,100:A>G -AMELIA:165:C03TUABXX:5:1101:17042:48299 - 1 134015 TCTAAAAATTTAACTTTGTCCTTTTTTCTTTTTCCCTCCCCCGTTTTCCCCCGGGTTTTTTTTTTTTTTTTTTTTTTTTGAGACGGAGTCTTGCTCTGTCA ########################################################B:36BB=6;DHJJJIIJJJJJJJJIHGIIJJJHHHHHFFFFFCCC 0 0:G>A,16:C>G,46:T>G,47:T>G,48:T>G,49:T>C,50:T>C,53:T>C,55:C>T,56:C>T,57:G>T,59:A>C,60:A>C,61:T>C,62:G>C,63:A>C,64:G>T,65:G>C,67:A>C,68:G>T,69:G>T,70:G>T,73:T>C,74:G>T,75:A>T,76:C>T,83:A>G,84:A>T,87:T>C,89:C>A,90:C>T,91:G>T,92:A>T,94:G>A,95:T>A,96:G>A,97:G>A,98:A>T,100:C>T -AMELIA:165:C03TUABXX:5:1101:15517:62035 + 1 414128 AGCCTGGGCAACAGAGCAAGACTCTGTCTCAAAAAAAAAAAAAAAAAAGGGCAGAGAGGAATTTAATTAATAATATAGTCAGCCTGAGGTATCCATTAGTC CCCFFFFFHHHHHJJJJJIJJJJJJJGIJJJIJJJJJJJJHFDDDDDB##################################################### 0 21:T>C,26:A>T,48:A>G,50:A>G,51:A>C,53:A>G,54:G>A,55:A>G,57:A>G,58:A>G,59:G>A,60:G>A,61:A>T,62:G>T,63:C>T,64:C>A,65:T>A,66:C>T,68:T>A,69:T>A,70:G>T,71:C>A,72:C>A,74:C>A,76:T>A,77:T>G,78:A>T,80:C>A,81:C>G,82:T>C,83:A>C,84:A>T,85:T>G,86:C>A,87:T>G,89:G>T,90:G>A,91:C>T,92:A>C,93:A>C,94:G>A,95:C>T,96:C>T,97:T>A,98:T>G,99:G>T -AMELIA:165:C03TUABXX:5:1101:6460:26694 - 1 329183 GGGGGGGGGGAGGTTGAAGGGAGCCAAAATTTGCCCCCTGCCCCCCAGCCTGGGTGAAAAAGAAAAACCCGGTCTCAAAAAAAAAAGAAAAAAAAAAAAAT ############################################################################DDFJJJIJHD;JHHHHHFFFFFCCC 0 0:A>T,14:A>G,32:T>C,35:G>A,38:C>A,41:G>A,43:C>A,46:C>T,57:T>C,59:A>C,64:A>C,67:A>C,68:C>G,69:G>T,70:C>T,73:G>A,81:T>G,84:C>A,92:A>G,93:A>G,97:C>G,100:C>G -AMELIA:165:C03TUABXX:5:1101:13936:64280 - 1 325734 TGGACCCTCCAGACCCAGATGGTGTCTCACTGTGGCATCCTCAGTTGAAGCTCCTGCCTTTCGGCAGCCTCTCCAGGCCCAGCTCCTCCTGCCTCCCAGTG #######A993CA9A:@?@?BCC@>5A:C?9,,>25;;;@@3C>@6>>B==B8;?;@>EIHFHGFDFB?*F?GGBDDAEBDHEH>GIGFFADGFFDBD@@@ 0 55:C>T,56:G>T -AMELIA:165:C03TUABXX:5:1101:6797:10552 - 1 576483 AGGAGAGACTGGGTGGGTGCTATAAGTGGGGGTGGGCATATAAAGGCTGCTGTTCATCTTTCCGCCTTGGCTTCCCAAAGTGCTGGGATTACAGGCGTGAG ##################################################################C3;E?C383EE>B@GC::F>EFC22<?DD?D?1?? 0 29:C>T,38:T>C,40:C>T,41:G>T,46:G>C,48:G>T,49:C>G,52:C>G,53:A>T,54:G>C,55:T>G,56:C>G,57:C>A,58:T>A,59:C>A,62:C>T,63:C>A,64:T>C,65:C>G,66:T>G,68:G>T,69:T>G,70:A>G,73:A>G,74:C>T,76:G>A,79:T>A,80:G>T,81:T>C,84:C>G,85:A>G,86:C>G,88:T>G,89:T>G,92:G>C,93:T>A,94:C>G,99:A>G,100:T>A -AMELIA:165:C03TUABXX:5:1101:8743:50636 + 1 142312 GCAATATGTGGTGCTCATGAACAAAGCAAACATTAAAGTCAGACCAGACCCAACATTTGACTCAGTCTTAATATCCAGGTGAGCCTGCGCAAATCATTCAT CCCFFFFFHHHFHJJJJJJIIJJJJJJJJJJJJJJIJJIJIJJJJJJJJJJJJJJJJJJJJJIJJJJJJIJJJJHHHHHEEDEFFEEDDDDDDDDCDEEED 1 -AMELIA:165:C03TUABXX:5:1101:15871:55839 + 1 682091 GGGAAGGCTGACATGGTAGGATTGCTTGAGCCTGGGAGTTTGAGGCTATAATGAGCCATGATAGGACCACTGAACTCCATCCTGAGTGACAGGGCAAGGTC @C@FFFBFHHHHHIIFCFEEFGHGHHIIJJJJGIJJEHDFHIEHIIIEHIJIIHEGFHGEGHIIGGGHFFFEHDFDDEEEEEEECDCCDDCCBBD?BDD:> 1 -AMELIA:165:C03TUABXX:5:1101:6309:29014 - 1 146406 AGGCTGACATGGTAGGATTGCTTGAGCCTGGGAGTTTGAGGCTATAATGAGCCATGATAGGACCACTGAACTCCATCCTGAGTGACAGGGCAAGGTCCTGT ########@52;>;;;@;>>@?@AFEECAEC@@7=AFF>FGF<CIFD9D9)?*B909????:DGCFFGBG?;;BIG?GFE?C@EAEC2<:<+FB;DAB@?? 1 -AMELIA:165:C03TUABXX:5:1101:11676:33354 + 1 149508 CTCTACTAAAAATACAAAAATTAGCCGGGCATGGTGGTGGGGCCCTGGAATCCCAGCAACTCGGGAGGCTGGGGCAGGAGAATGGCGTGAACCTGGGAGGC ?;?+4AA?DFBH>?FAFIEID<<F;2<:):?DGH*0?0?D############################################################# 1 25:T>C,29:T>C,37:C>T,41:C>G,42:A>C,47:T>G,57:T>A,71:A>G,83:T>G,86:T>G,93:C>T,94:A>G,96:A>G,100:T>C -AMELIA:165:C03TUABXX:5:1101:3488:13611 + 1 164365 CATGGGTTCACGTCTGTAATCTCAGCACTCTGGGAGGCCGAGGCGGGAAGATCACTTGATATCAGGAGTCGAGGCGGGAAGATCACTTGACGTCAGGAGTT CCCFFFDFHHHHHJJJJJJJJJJJJIJJIIIJJJHIJJJJGIJJJHDBDDBD@CCDDDDDEEDEEDDD?CBBDDBDBB5<@<CCDDDDCCD><<BDDCD9A 1 -AMELIA:165:C03TUABXX:5:1101:14811:37223 - 1 561943 CGGGGAGCAGGGAGGCGCCAAGCGACACCCTTTTCCCCGCGGTGTCTTTTTCGGGGTCCTCCCCCCGTTTTTTTTTTTTTTTTTTTTTTTTTTGAGCTGGA ########################################################################B@@BCEE@@@DAFC:?FDF<>DD:B=@@? 0 4:A>C,28:C>T,32:C>T,34:A>G,36:A>C,37:A>C,38:A>C,39:T>C,40:T>C,41:A>T,42:A>C,46:A>G,48:A>G,49:T>C,50:A>T,51:C>T,52:A>T,53:A>T,54:G>T,55:G>C,59:C>G,60:C>G,61:G>C,63:T>C,64:A>C,65:T>C,66:G>C,68:C>T,69:C>T,71:T>C,73:G>C,74:T>A,75:A>C,76:C>A,77:C>G,78:G>C,79:A>G,81:C>A,84:T>G,87:T>G,89:C>G,90:C>G,94:A>G,95:T>A,97:A>G,100:T>C -AMELIA:165:C03TUABXX:5:1101:19656:62826 - 1 443302 CTGGAGTTCAGACTTGTGCTCTCCCGTTCTTCTCGATCTCCTGCCCTCGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCAC ########################################################BFG<HF@???GHFHG<IGGGFD?>FA4BBGIGFDDDHBDDDD?:? 0 46:T>C,53:T>C,57:A>C,63:G>C,67:T>C,71:G>T,72:G>C,74:A>T,76:G>C,77:A>C,79:C>T,80:G>C,81:A>T,82:T>C,83:T>G,84:G>T,85:T>G,86:G>T,87:C>T,90:C>G,91:T>A,92:T>C,94:G>T,96:G>A,98:C>G,99:A>T,100:G>C -AMELIA:165:C03TUABXX:5:1101:6797:10552 - 1 576483 AGGAGAGACTGGGTGGGTGCTATAAGTGGGGGTGGGCATATAAAGGCTGCTGTTCATCTTTCCGCCTTGGCTTCCCAAAGTGCTGGGATTACAGGCGTGAG ##################################################################C3;E?C383EE>B@GC::F>EFC22<?DD?D?1?? 0 29:C>T,38:T>C,40:C>T,41:G>T,46:G>C,48:G>T,49:C>G,52:C>G,53:A>T,54:G>C,55:T>G,56:C>G,57:C>A,58:T>A,59:C>A,62:C>T,63:C>A,64:T>C,65:C>G,66:T>G,68:G>T,69:T>G,70:A>G,73:A>G,74:C>T,76:G>A,79:T>A,80:G>T,81:T>C,84:C>G,85:A>G,86:C>G,88:T>G,89:T>G,92:G>C,93:T>A,94:C>G,99:A>G,100:T>A -AMELIA:165:C03TUABXX:5:1101:18778:29474 + 1 526987 CCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTGCAGTGAGCCGAGATCCCGCCACTGCACTCCAGCCT C@@FDFEEHHHHHJIIJJIJJJHHIIJIGHIGGHJGIDIEIGGGDHIGGIIGIFBEDDBDBBBACDBCCDDAACD@BD<BCDDDDDBBCDDCDDDDDDDBC 0 -AMELIA:165:C03TUABXX:5:1101:10989:33205 - 1 710921 TAGAATTAGAGACATCTGGGCCAGGCACAGTGGCTGACGCCTGTAATCCTAACACTTTGGGAAGCCGAGGCAGGCAGATCACCTAAGGTCAGGAGTTCGAG EEEEEDEDDEDEDDDDDDDDDEEEEEEEFFFFHHHHJJJJJJJIJJIJIJIIIHJJJJJJJJJJJJJIJJJJJJJJJJJIHJJJJJJJHHHHHFFFFFCCC 1 -AMELIA:165:C03TUABXX:5:1101:16440:15447 - 1 676180 GCCTGGACGACGTAACTAAATCTCATCTCTACAAGGAAGAGGTGGGAGGATCACTTGAGCCCAGGAATTTGTGGCCAGCCTGGGCAACAAAAGAAGACCCC DDDDDDDDDDDDEDDDEEEEEEFFFFFFFHHGHGHJJJJJJIJJJJJJIJJIHJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJHHHHHFFFDFCCC 0 -AMELIA:165:C03TUABXX:5:1101:11887:26223 - 1 672555 TGAGTCGCTGTGAGGTCACCTACTGGAAATCACCAGCATCCCATTTCCCACTGGCAAAGAGCTCAGCACTGCCCCCTGGGAAACCAAACCTATGCCCAAAT ######################AAAAA>>A??AAA@>?9@;)AA=(A=7);=>C@9CDCC=(444B9/70))))A3?<+:A;C>DEAE?>DDBB@;A4;== 2 94:A>G -AMELIA:165:C03TUABXX:5:1101:13624:37788 + 1 324594 CAGCTCCATCGTTACAATGGCCTCTTTAGACCCAGCTCCTGCCTCCCAGCCTTCTCTCCAGGCCCTGAACTTTCTCAAGTCGACCTCACCAGGCCCAGCTC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJIJJJJJJJGJJJJJJJJJJJJJJJHIIJJJJJJHHHHHFFFFFDFEEEEDDDDDDDDDD?BDDBDDDCD 0 -AMELIA:165:C03TUABXX:5:1101:1670:60414 - 1 324821 ATCTGCCTCACTGTGGACCACCCAAGCCAAGCTCCCAACCTTTCAGCAGCTTCTACACACCCAGCTCCTGCCACCCAGTGGCCTCTTTAGGCCAAGCTCAT DDDDBBDCCCACDCA:9@91B?@?C>BC;BB@?CDFEDDHGFHHJIHEGGAIHGFDF<9FIIFIGGEHEGHGEHGG@GGIJIGFEHGBHHDF>DFFFF@@@ 0 81:C>A,96:A>G -AMELIA:165:C03TUABXX:5:1101:6413:54540 - 1 664425 AGAGGTCAACTTGAGGAGATTTTGGGCCTTCATAGGCCACCAGGAGGCAGCAGTTGGGACTAGAGAGGCTGACTTGAGTAAGTTTTGGGCCCGGAGATGAC DDDDCDCDEDDDDDDDDDDDBDDDDDEEEEEFFFFFHHHHHIJJJJJJJJJJJJJJJJJIJJJJJJJJJIIJJJJJJJIJJJJJIIJJHHGGHFFFFFC@C 0 33:T>G,99:C>G -AMELIA:165:C03TUABXX:5:1101:5381:23203 + 1 138827 AGCAGTTGTGCCTGTAGACCCAGCCAAGAGGAAGAGGTGGGCCTGGAGAAGCCCCCATGAGGCAGAGGTTGGGCCTGTAGACGCTGACAGGAGGCAGGAGC @@@FFBDFH?DHHIIIIGGIIIGIIIIIGIIGIIGIGBDGAHIIGHIGFHIEHIIIIEHFEHFFFDAB6@CBBCBBBCCCECBC@BBCCCC@BBBBBB?BB 1 -AMELIA:165:C03TUABXX:5:1101:13970:15259 + 1 325209 CCTGCCTCCTGTCAGCGTCTACAGGCCCAACCTCTGCCTCATGGGGGCTTCTCCAGGCCCACCTCTTCCTCTTGGCTGGGTCTACAGGCACAACTGCTGCC CCCFFFFFHHHHHJJIJDGIIJJJGIJJJIJJGJEGIIIJIGIIJIIIIJIJGHHHCEEFDDDCDDDDDDDDDDDCDBDDCCDACCCDDABCDDCCDCDCA 0 -AMELIA:165:C03TUABXX:5:1101:18642:29365 - 1 325339 GTCCAGCTCATGTCGGCCAATGTAGGCCCAAAACTTCCTCAAGTCAAACTCTCCAGGCCCACCTTCTGCTTCCCGGTGGCATCAACAGGCCCAGCTTTGAC DDDDDDDCCDDDDDDDDDFDDDDDDDDDDDECAECCEFFFFFDHHHHEECC6JJJJIJIGBJJJJJIJJIIJJJJIIJJJIGIJJJJJHHHHHFFFFFCCC 0 88:G>T -AMELIA:165:C03TUABXX:5:1101:13936:64280 - 1 325734 TGGACCCTCCAGACCCAGATGGTGTCTCACTGTGGCATCCTCAGTTGAAGCTCCTGCCTTTCGGCAGCCTCTCCAGGCCCAGCTCCTCCTGCCTCCCAGTG #######A993CA9A:@?@?BCC@>5A:C?9,,>25;;;@@3C>@6>>B==B8;?;@>EIHFHGFDFB?*F?GGBDDAEBDHEH>GIGFFADGFFDBD@@@ 0 55:C>T,56:G>T -AMELIA:165:C03TUABXX:5:1101:7560:35532 + 1 325877 CCCAAGCCCCGCTTTTGACTTTCGGTGGCCTCTGCAGGCCTCGACAAGGCCCAGCCTCCTGCCTCCCGAAGGCCTGCACAGGCCCAGCCTCTGCCTCACAG @@@DD?DBC@F?FEHDC=?CFFHBA:?FGHI@GIG?B??F38@AA@BH=E?;?=2?;@?C>;A>;=<8@B;5??AB<?:>32(82(((2(93>>ACC>?## 0 -AMELIA:165:C03TUABXX:5:1101:5732:9122 + 1 326001 TGTTGCTTCACTGCGGCCTCCCGAGTCCAAAGCTCCTGCCTCTCGGCCGCTTCGGCAGGCCCAGCTCCCGCCTGCCAGTGGCCTCTTCAGGCCCATGGGGC CCCFFFFFHHHHHJJJJJJJJJJJJHGHIJJJJJJIJJJJJJJJFJJJIGHFFFDDBBDBDDDDDDDDDDDDBBDBCCCDCDDDDDDDEDDDDD@CCCDDD 0 -AMELIA:165:C03TUABXX:5:1101:7628:28013 - 1 663374 GACGCATCCAGGAAGAAGAGCTGGACCCGGAGAGGACGCCCGGAGGGTGCAAGTGGCTCTGGAGAGGCCGACTTGAGGAGGTTCTGGGCCCGGAGAGGCCG ############################B?A>BB@;/?:=/=;?DEA?=)))EAC@=@7F@DF>EF@8B?0?GD?IFC@=DG=BFC8<@<:;ADDD?D?=@ 1 44:G>C,66:A>G,76:G>A,94:A>T,97:C>G -AMELIA:165:C03TUABXX:5:1101:3031:11063 + 1 326249 CCAGGCCCGATTCCTGCCTCTCAACAACCTCTTTGGACTCAGTGCCTACCCATCTCCTGGCGGCCTTGGTCGGCCCACAGCTTCCTCAAGCCAAGCTCCCC @@CFFFFFDDHFFIGGIJJGIGIGEEGHIGIGGCGFEHIIBFDGGHCGIJIDGGIIGGD=DEF>B@DDCCCB:B@<?B<BBDDDDCCCCCC?C?<?@CCDB 0 -AMELIA:165:C03TUABXX:5:1101:2464:49300 - 1 326273 CAACCTCTTTGGACTCAGTGCCTACCCATCTCCTGGCGGCATTGGTCGGCCCACAGCTTCCTCAAGCCAAGCTCCCCAGGCCCAGGTCAGGCCTCACGGTG A?@A?99CCCCCCCCCCBCCB?>3A@C>1@??-BBB@>9@>BBCA>FHGIEACHGEEC=;FBEIIIHGGEHEBIIIGFEEF@EIGGBEFFHBDDADDD@@; 0 60:C>A -AMELIA:165:C03TUABXX:5:1101:13449:20368 - 1 564462 GGGGAGTCCGAACTAGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCGAATACACAAACATTATTATAATAAACAC DDDDDDDDDEEDDDDDCDDDDDDDDDDDBEDCDEDDDBDDFHHHIIIJJJJJJJJJJJJIJIIJIJJJJJJJJJIJHJHJJJJIIIHJHHHHHFFFFDBB@ 0 100:A>G -AMELIA:165:C03TUABXX:5:1101:14456:4016 - 1 564474 CTAGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCGAATACACAAACATTATTATAATAAACACCCTCACCACTAC ?C@CCCCBCBCCCCCABEDC?ECBBBB@;BB@?CCEEBDFBIGIIHBGIHGEGGCEHDHHGHG???CGGHGDAGGGGEHBEH?DECA2AFHFFBDDBD@@@ 0 -AMELIA:165:C03TUABXX:5:1101:17334:7368 - 1 564478 TCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCGAATACACAAACATTATTATAATAAACACCCTCACCACTACAATC >DCDDDDDDDDDDDDDDECB@<DDDBBDBBDDDFFHEIIJJIJJIHIHGJJIJIIGJJIGFGDJIJJJJJJIGGIEJGGHFFC<IHECFAHFFDEDFD@@B 0 -AMELIA:165:C03TUABXX:5:1101:5194:63672 - 1 564482 AGGCTTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCGAATACACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCC DDDDDDDDDEDDDEDDBBDDDDDDD@DDDCDFGHJIJIIGJJJJJJJIJJIJJJJIGGGJJJJJJJJJIIJIIJJJHHFCIGGDGCIHFHFFHFFFFFBC@ 0 -AMELIA:165:C03TUABXX:5:1101:17747:9848 - 1 564492 ATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCGAATACACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCTAGGAACAAC :4<@A800<B>><<B=8368;939;HHHEE@4C=4AG@IECAHF??0?09?90?9<HG<G?9D@HG???1IGHFA3FA3ABABC<<C<::A:A=AA?B@@? 0 -AMELIA:165:C03TUABXX:5:1101:16033:12704 - 1 564499 ACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCGAATACACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGAC 9CBBBBBB>CCCBBCCCEFFFFFFFEHHGIIEIIIIIHFFHFGIHBIIGIIHCIIHHHGFF?1IGGEGEGFCHFGHHFFEC>IIGIIHHFHFCFFFFD@?@ 0 2:A>G -AMELIA:165:C03TUABXX:5:1101:6451:55200 - 1 564510 CCCTTCGCCCTATTCTTCATAGCCGAATACACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCC @9B<BBCBCCCCCCCAACCCCBCEFCDDC@=AEEHHGDCEE@GEGCAHGAFBHGFBB9DF?DB?HIIIIIIIIHGIIIIGEAGGHEBHHFFFDB?DDD@@@ 0 13:A>G -AMELIA:165:C03TUABXX:5:1101:5786:60442 - 1 564510 CCCTTCGCCCTATTCTTCATAGCCGAATACACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCC DDDDDDDDCDDDDDDEDDDDDDCEFDFD@DHFHHHHJJIJJIJGHIHHHAC6HHGAFBHFFIIIJHHDHEHJJIJJJJJIIIJIHIHHHHGHHDDFDFCB@ 0 13:A>G -AMELIA:165:C03TUABXX:5:1101:6531:61902 + 1 564516 GCCCTATTCTTCATAGCCGAATACACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACT @@@BDDEDHHGHHJGIGIIGIIJJJJJICEGIGIGHCFFIGIGHGCD?GBGCDHDDHDF@@EHJEGGGGGHCHEEBEDC>AADCBDDB?A@CCDDCBCDAC 0 81:A>G -AMELIA:165:C03TUABXX:5:1101:8689:7265 - 1 564520 TATTCTTCATAGCCGAATACACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTAC DDDDDEDDDDDDDDFEFDCDBEEEFEEEFFFFFFFHHHHAD@JJHFHFIFCIIHJJIGIGHJIIJJJJJJJJJJJJIIJJIHFIGJGJHHHHHFEFFF@B@ 0 23:A>G -AMELIA:165:C03TUABXX:5:1101:18212:43965 + 1 564531 GCCGAATACACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTACACAACATATTT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJIJHJJIJJJJJIJJJJJJJJJJGIIJJJJJJIJJJHHFFFEEEEEDDDDDDDDDDDDDDDDDDCEEE 0 66:A>G -AMELIA:165:C03TUABXX:5:1101:6615:4172 - 1 564532 CCGAATACACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTT BDDDDCCDBDDDDDEDEDDEEEDECCA>;5FHEECHIGHHDEGIIGIIIHGHGGIJIHEJHHCDDDDIGDCHEHFGDJJIFBAGAHFEHDDFHDDADD@@@ 0 35:A>G -AMELIA:165:C03TUABXX:5:1101:16689:19546 - 1 564532 CCGAATACACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTT DDDDCCCCBDDDCDEDEEEEDCCA@>;9;,FHHEE@E@=GGFJIHCFBCIIHIJIIIGIIIHG@??::FC:D@ECHEBHIHIHHAGEGFDHFCDDDFF@@? 0 35:A>G -AMELIA:165:C03TUABXX:5:1101:18225:47825 + 1 564534 GAATACACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGT C@CFFFFFHHHHHJJJJJJJIJJJJJJIJJJJJJJJIJJJJJIJIIJIJIIIJJJJIJJJJIJJJJJJHHHFDFFDDCCCCDDDDDDDDDDDDDDEEEEDB 0 63:A>G -AMELIA:165:C03TUABXX:5:1101:19851:25771 + 1 564539 CACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCA @@@DDDF?HHFAHGGIGIIIIIGGIIGIBHIIIIIIIICHGCAFHGHCBGEHIIGIGDHGHGHHAHCHEHHFBB@;>ACCECCCCBCCCCDDDECCCECCC 0 58:A>G -AMELIA:165:C03TUABXX:5:1101:20376:57756 - 1 564539 CACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCA CBDEEDDEDEFEEEEDDDDB>A;CADCB;FHHGHHHJIIJJIJJJJJJIHJIIJJJJIHGIGG?HFHGJJJJJIJJJJJHGGC,JIJJGHHHHFFFFDCCB 0 42:A>G -AMELIA:165:C03TUABXX:5:1101:1712:35753 + 1 564542 AAACATTATTATAATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGA @CCFFFFFHHHGGEGIJIIIIIJIJIJIGIGFHIIGGIEGHCDDGHGIGGGIIIIJJIJIGIGGIIJHHC?7@DFECEDEDE=?B?CDC@55<@@CACA@D 0 55:A>G -AMELIA:165:C03TUABXX:5:1101:8722:10321 - 1 564555 ATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAAC CA<<585BBA:?;CCDCDCC@CAA><FDBFFHHAHHIIHCIHEGHHFBGFD6IIIIHIHHIGECDGGIHDHF?FHBGHHBEBIIIIIIHFHDHBB;DB@@@ 0 2:G>A,58:A>G -AMELIA:165:C03TUABXX:5:1101:6265:16886 - 1 564555 ATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAAC DCC?9?5DDBA?;BDCDEDEEEEECDFECFFHHEHEDIJJIGHFIHFBGFF@JJIJJIIDJIIGDHHJIGIJJJJJJJJIHFJJJJHGHHHHHFFDFF@@@ 0 2:G>A,58:A>G -AMELIA:165:C03TUABXX:5:1101:9771:64146 + 1 564564 CTCACCACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACCTCCCTGTT CCCFFFFFHHHHGJIJJJIJJJJGIJIGIJGIJJGIJIEIIIIIIJJJJHJJJJIIJIIJJEGHHHFHHHEFDFFFDDEDEDDDDDDDDDEDDDDBDDDCD 0 33:A>G,89:G>A -AMELIA:165:C03TUABXX:5:1101:8328:47229 - 1 564586 GAACAACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATAC DBACDDEEEBD@?BCA@DBDDDEFFDA?=EEHD@GEHF@IHGIGJHGFHHGJJIHFBGGHGGHHBHGGDGE;JIGIIIIIJJJJIIIGHHHHFFFFFF@B@ 0 33:G>A,89:A>G -AMELIA:165:C03TUABXX:5:1101:17339:46783 - 1 564591 ACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCG EEEEDDDDDDCCDDDDDDDDEDDCDEDFCDCDDDEFDDDDDEEDCCFFFFD@HHHHEJJIJGHFIHCJJJJJJJJJJJJJJJIJJJJIHHHHFDDFFFCCB 0 38:G>A,94:A>G -AMELIA:165:C03TUABXX:5:1101:16422:52679 - 1 564602 ACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGCCCCTACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCCGCTACG #B<DBDDDDECDCDCCC?@CDEEC>C>ADC@DC@>BBA75'DCCC@DDCDDDBBB?DFD@DBCHEHEFJIGGJIIJIFGIHEF?IGEHFHFHFDFFDB=@@ 0 49:G>A,60:A>C -AMELIA:165:C03TUABXX:5:1101:12415:10948 - 1 564604 TCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCCGCTACGAC 9DDDDDDEDD@DEDDCDDDC@EDCDDDDDDCBCCDDCC>DDCC:CAABCA;B??DDEDFFFFFHHHFHHJJJJIGIIJIHF<JJIIIIHF>GHFFFDFCC@ 0 51:G>A -AMELIA:165:C03TUABXX:5:1101:5317:25747 - 1 564635 CACCAAGACCCTACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCC DDDDDDCCDDDCADCCBC<+DDBDDCDDDCDDEEDDDDDDDDDDDEDDDB=DJJIJJIIHJJJJIHJIHJJIHIHIHHCFAJIHJJIJGHHHHFFDFFCCC 0 82:G>A -AMELIA:165:C03TUABXX:5:1101:5006:49754 - 1 564672 ATTCGAACAGCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACTTATATGATATGTCTC DDDDDDDDEDDDB>DDDDDDDDDDDDFFFHHEJIGDIHIGHFF<JIGJJJJJJJJJJIGJIJJHGCHHEHFHAJJJIJJJJIHFJJIIHGHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:10445:44437 - 1 564682 CATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACTTATATGATATGTCTCCATACCCATT DDB<DDDDDDDDDDDDDDDDEDFFCEFHHHHE=CIHFJJIIHIJJJIGDJJIJIHDHFDHDH?JJJJJJJJJJIJJJJJJJJJJJJJJHHHHHEDFFFCB@ 0 -AMELIA:165:C03TUABXX:5:1101:11503:43058 - 1 564691 GATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACTTATATGATATGTCTCCATACCCATTACAATCTCC >B>>BDB@A=?>=CCA;A@C@?;?5BEHHHAECJHHFBB=HHFGGB8FB?F?B:IGHFGHFGHGIHGGHGEHGHHIHEDHCGFEEAGIHHDFHEDDAD?@@ 0 -AMELIA:165:C03TUABXX:5:1101:14447:11274 + 1 565034 CAACACGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGC @@@FFFFDFDDDHGIGGEHHCHHIIIIGIGGHEGGIGGHGFF?FHGBIIGIIIIGIEIIHEDEFFFFFDDCCD@CA@CCCCCCDCDDCDDDDDDDDDDDDC 0 4:C>A -AMELIA:165:C03TUABXX:5:1101:14195:33093 + 1 565034 CAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGC B@@DDFDAFHHGHIJJJIIJIIIIJJJIIGIIIIIIIIIIHIIJIGHGIJJIJFJJJJHHFFFFFEEEDBDDDDDCCADDDDDDCDCCDDDDDCCCDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:8812:42493 + 1 565034 CAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGC CCCFFFFDHGHHHJJJJJJJJJJJJJJJJJJJJJIJJJJJJJIJJJJJJJJJJJJJJJHHFFFFFEEEDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:19983:49008 + 1 565034 CAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGC @CCFFFFFHHHHHIGIJJJJJJJJJJIIIIJJJJJGIIIEHGIIFIIJIJJJJJIIGIHHFFFDFEEEDBDDCCCCDDDDDCCCDCDDDDCDDDDDCDDCC 0 -AMELIA:165:C03TUABXX:5:1101:17655:2032 + 1 565037 NCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTT #1:D=A?BCDDFAEGIIB@BCE:B<CF@?:FDFEGDFGBGC<?FEBDFFDA@FFFIAECA=CAEB@=AAC@>@;@A>5>>>?BB@A@BA:>@>@BBB:ABB 0 0:C>N -AMELIA:165:C03TUABXX:5:1101:12424:6001 + 1 565037 CCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTT C@@FDFFFGHHGHJJIGEHJJIIGJEGIJJHHIIIJCIIGIJJHGIFGIIIIIGIIJEFFFHHHFDDEECDDD>CCCCDCDCCDDDDDDDDCDDDDCDCCC 0 -AMELIA:165:C03TUABXX:5:1101:15179:54060 + 1 565037 CCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJIJJJJIJJJJJJJJHHHHHHHFDDEEEDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:4131:14575 + 1 565038 CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJIJJJJJJJJJJIJJJJJJJJJJJJJJJHHHHHHFDEEEEDDDDDCDDDDDDDDDDDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:14283:42154 + 1 565038 CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTT CCCFFFFFHHHHHJJJJJJJJJJJJJJIJJJJJJJJJJJIJIJJJJJJJJJJJJJJJJHHHHHHFDEEEEDDCCDDDDDDDDDDDDDDDDDDDDDDDDDDC 0 -AMELIA:165:C03TUABXX:5:1101:7649:3637 + 1 565039 CGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTA CCBFFFFFHGHFHGHIIJJJIIIIJIJJGIJGFFIIDFGIJHGGJJJJIJJIIJIIJJJHHHFFFCEAEED>ACDCCDDDDDDDDDDDDCCCCDDDDDDDC 0 -AMELIA:165:C03TUABXX:5:1101:17027:3351 - 1 565043 ATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCC DDCCCDCCDCCDCDDCDDDDDDCCA@AEDEECD?FHHJJJJJJJJJJIIIJJIIJIJIHF?IHIJIJJGHGGHGIIIIIGHHHIGJIJGHGHGFFFFF@CB 0 -AMELIA:165:C03TUABXX:5:1101:2923:59080 + 1 565050 CTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTCTA CCCFFFFFHHHHHJJJIJJJJJJIJJJJJJJJJJJJJJJJJJJJJJGJJJJJJHHHHHFFFFFFDEEDEEDDDDDDDDDDDDDDDDDDDDDDEEEDDDDEE 0 -AMELIA:165:C03TUABXX:5:1101:3544:36554 + 1 565053 CCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTCTAACC CC@FFFFFGHHFHIHGEGIJJJJIJGHIGIIIIJGGIIIJJJJJJIJJIJJIJHHHHEHFFFFFCDCCEEDDDDCDDCDDDDDDDDDDDEEDDCCCDDCCD 0 -AMELIA:165:C03TUABXX:5:1101:19486:58467 + 1 565054 CATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTCTAACCA CCCFFFFFHHHHHJJJJJJIJJEJJJIJJJJJJJJJIJIJJJIJJJJJIJJJJHHHHHHFFFFFEEEEEEDDDDDDDDDDDDDDDDDDEEEDDDDEEEDDD 0 -AMELIA:165:C03TUABXX:5:1101:6592:19626 + 1 565055 ATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTCTAACCAA CCCFFFFFHHHGHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFEEEEDEEDDDDDDDDDDDDDDDDDEEEDDDDEEEDDDD 0 -AMELIA:165:C03TUABXX:5:1101:16094:17052 + 1 565062 CAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTCTAACCAAAAAAATA @<@DDDDDBFF??F@GIG+@@GHG@9EAHB0:@D3DFBDGH=;@FE;@7=ACHIHF<A??@C>?D6>>>@C>;:>@C@CCC;BCCC>C>C@A:1<<<9@BC 0 -AMELIA:165:C03TUABXX:5:1101:4407:63853 + 1 565066 GCCACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTCTAACCAAAAAAATAAACC @CCFFFDDHDDHDF=GHIDFEHGE@FFGGE::DD<GDDFCBFHCHGEACDEED>DA?AEHHDFCFFCCA>CAACCCC;@C;>ACAACBCB@?B><BC@CCC 0 0:C>G,1:A>C -AMELIA:165:C03TUABXX:5:1101:20917:10211 - 1 565070 CTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTCTAACCAAAAAAATAAACCCTCG DEDEEDBDDDDDDDDDDDDDDDEDDDDDEEEEC@FFFHHHGGIIJJJJJJJJJIHDJJJJIIJJJJJJIJJJIJJJJJJIJIJJJJJJGHHHHFDFFFB@B 0 -AMELIA:165:C03TUABXX:5:1101:8952:59868 + 1 565075 CACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTCTAACCAAAAAAATAAACCCTCGTTCCA @@CDDDFFHGDABGCGEBHHGGFHIIIIIIIFGGIIEGHEHIIHCHGIIICHIFIHHHHCADFFDCBCC>A@CAB5@CDDDBBBBBBCDDCCBBBDDBBCC 0 -AMELIA:165:C03TUABXX:5:1101:5290:63611 - 1 565086 GCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTCTAACCAAAAAAATAAACCCTCGTTCCACAGAAGCTGCC ####A>43AA<5:,>5,5:555A=;;;5(3DDDAA:@@;)>A;;@=76))C=..3CA@@=7=.=DD8D?@D?19@CEBE<?6:@A2?3BDDADB:AD=??? 0 -AMELIA:165:C03TUABXX:5:1101:1768:27227 + 1 565087 CTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTCTAACCAAAAAAATAAACCCTCGTTCCACAGAAGCTGCCA @C@FFFFFHHHHHJJJJJJIIJJIIIGHHJJJJIIJJJJIIIIIJJJJJJJIGIJJJGIHIJGJGIJGIHHHFDDDCDDDDDDDBDDDDDDDDDCDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:15632:25198 + 1 565119 AAATAAACATGCTAGCTTTTATTCCAGTTCTAACCAAAAAAATAAACCCTCGTTCCACAGAAGCTGCCATCAAGTATTTCCTCACGCAAGCAACCGCATCC @<@AADEDD8AD>E@GECDECHHGEBFCC?FHHGFHIIGHGIICBDHE@FGEIBHGIGEGGHEC;ACBFCD@CC6;BCE>>@@CBBBCBBCBCC8<.<BB? 0 -AMELIA:165:C03TUABXX:5:1101:14710:10393 + 1 565129 GCTAGCTTTTATTCCAGTTCTAACCAAAAAAATAAACCCTCGTTCCACAGAAGCTGCCATCAAGTATTTCCTCACGCAAGCAACCGCATCCATAATCATTC ?8:=AB:ABFFAB?AAA2<IFAFHFICGCBGFFFIFF;FEF@F18=CFF>AAFCDC=@@)=CE7)777?3?;?A@@;;@@9<?<@69B0&9A:A@###### 0 97:C>A -AMELIA:165:C03TUABXX:5:1101:14042:48034 + 1 565134 CTTTTATTCCAGTTCTAACCAAAAAAATAAACCCTCGTTCCACAGAAGCTGCCATCAAGTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATA CCCFFFFFHHHHHJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJEHHHHHHHFFFFFDDDDDDDDDDDDDDDDDDDDDDDDDDEEC 0 -AMELIA:165:C03TUABXX:5:1101:2589:2133 + 1 565155 AAAAAATAAACCCTCGTTCCACAGAAGCTGCCATCAAGTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATA CCCFFFFFHHHHHJJJIIJJJJJJJJJJJJJJJJJJJJFHIJJJJJJJJJJJJJJJIJJHFFFDEEEEDDDDDDDDDEEDDDDEDDDEDDDDEDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:4087:57681 + 1 565167 CTCGTTCCACAGAAGCTGCCATCAAGTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAA CCCFFFFFHHHHHJJJJJJJJJJJJGGIJJJJJJJIJJIJJJIJJJJJJJJJJJJJJJJJJHHHHHHFFFFFFEEDEEEEEDDDDDDDEEEDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:5754:6451 - 1 565172 TCCACAGAAGCTGCCATCAAGTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAAC CDDCDDDDDDDDCDDDCDDDCCACBCDDCDDFFHHHHHG@IJJJIGIIJIJIGIHCEHEJIJIIIIJIIHEIGHCGIJJJHFHCHEHHHHFFHFFFFFCBB 0 -AMELIA:165:C03TUABXX:5:1101:17395:16823 - 1 565172 TCCACAGAAGCTGCCATCAAGTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAAC CDDEDDDDDDDDDDDDCDDDDDDDDDDCACBDFGHHHGIIIIIIGGIIHIIIGIGGFIEIHFGHIGIIIIIIIIIIIIIGHHE<HFG@HHHHHFFFFFC@@ 0 -AMELIA:165:C03TUABXX:5:1101:2679:29962 - 1 565173 CCACAGAAGCTGCCATCAAGTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACC CDCDDDDCDBDDDDDDDDDDDDCA<BDBDCFFGHHHGIIJJIGHGIJIIIHEIIHCJIJIGJIIFHEIHHIJIJJJIJJGHHCGDIGJHHHFHFFDDD?@@ 0 -AMELIA:165:C03TUABXX:5:1101:16178:53296 + 1 565174 CACAGAAGCTGCCATCAAGTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCA CCCFFFFFHFHHHJJJJJJHGHIJJJJJJIJJJEGIJGIIJIGIJJGIIGIJIGIIIIGGCIJHHGHEHDFFFDC@CAEEEEEEDDDDDBBBDACDCCDBD 0 -AMELIA:165:C03TUABXX:5:1101:8205:37274 - 1 565175 ACAGAAGCTGCCATCAAGTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCAT >CCCCCBBCCCCCCCCCCCCCBCBBB?CCDEEHHHHGGGIGGEIIICHCFIEGIHEIIGHIHHCHHG@EHHDGFIIHGHFAF:F?IHHHHHFHDDDAD@@? 0 -AMELIA:165:C03TUABXX:5:1101:11194:16014 - 1 565178 GAAGCTGCCATCAAGTATTTCCTCGCGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAAC DDDDDDDDDDDDDDDDDDDBDB?9'CDFFFFFHHJJIIHFJHGBFGFIHIGJGJIIJJJGIHJIGIIJIJJJJIIIGEHFHFJJJJJJHHHHHDFFFFCBB 0 76:A>G -AMELIA:165:C03TUABXX:5:1101:20490:46953 + 1 565178 GAAGCTGCCATCAAGTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAAC @@@FFFFFHHGGHJJEGHGIIHGIJJJJJIJIIIJJJJJIJIGICHEGIJJJJJJGIJIEGIJIHGFHHHFEFFFFEEEEEDEDDDDDDDCDCCCDBDDCD 0 -AMELIA:165:C03TUABXX:5:1101:14176:62282 + 1 565180 AGCTGCCATCAAGTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCA CCCFFFFFHHHHHEHIJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJIJJJIJHHHHHHFFFFFFEEEEDDDDDDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:15202:19438 + 1 565182 CTGCCATCAAGTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAAT CCCFFFFFHHHFHJJJJJJJJJJJJJJIJJJJJIJJJJJIIJJJJJJJJJJJJJJJIJJJJJJJJHHHHHHFFFFFFEEEDCDDDDDDDDDDDDDDDDDDC 0 -AMELIA:165:C03TUABXX:5:1101:3794:62600 - 1 565184 GCCATCAAGTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATAC DDDDDDDDDDDCDCDDBBBDDEDECBEDHGJIIIIIGIIIIJJJIJIJIJJJJJIGIIGJIGEIGGGIGHGCIHIGIIIHJJJIJJHHHHHDHEFFFF@@B 0 -AMELIA:165:C03TUABXX:5:1101:4930:21100 + 1 565187 ATCAAGTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTAC @@@FFF=AFDBFDHGGGGAFEGHHHIIIIIEHIIICBGG??FHGGGHIIIIEGIGEHIIGCDHIEHECHEHHBDD>A>BBBDCDCDDDCCCDC@AACCCAC 0 98:C>T -AMELIA:165:C03TUABXX:5:1101:5798:58419 - 1 565187 ATCAAGTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTAC CDDDDDDDCA>DDDDBDDDEEEDFDFHHHGIIJJIIHGJJIGIIJJJJJJIGFJIJJJIIJJIJIIHFCHGIGJJJJJJJJJJJHJJIHHFHHFFFDF@@B 0 2:C>T -AMELIA:165:C03TUABXX:5:1101:19721:5927 - 1 565192 GTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGATAATGAACCATAACCAATACTACCAATC CCDDACDDDDDDDDDDDBA9FFDECBFEHEHAEIHEGGGJJIIJJJIHIHHIHGIJIHJGGGFBHHIEJJJJJGIJJIHFEHHEHHJIHFCAFB?FFD@;@ 0 7:C>T,27:C>T -AMELIA:165:C03TUABXX:5:1101:19523:43929 + 1 565192 GTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATC @@@FFBDEHHFHGEIIHGHHIHEHGDGHGEHGHIDHEHGGIIEHHDGFDGCGEGGHGIIIGCGHGEFEEHEFCDDBDDCCDD@DCCCCCBCCCC@C<@C>> 0 93:C>T -AMELIA:165:C03TUABXX:5:1101:6961:12869 + 1 565199 CTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTC @??DDDA?@C?ADFG?EDF@C?FDHCEHGBA?FBB?BABFDEAGICFGIG@<@F==CGI@@@CEAHBAB;(6>;?55;>>5:ACCA>CA?:>:?C4>>@C> 0 86:C>T -AMELIA:165:C03TUABXX:5:1101:1413:32291 - 1 565200 TCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTCA BDDDDDDDDDDDDDEEFFFFFFHGFHHGGIGJJJJJJIIIJJIIIGJJIIJIGHFBHAHGJJJIIJJJIJJIJJJIJHJJJJHGHGGJHHHHHFFFDF@CB 0 15:C>T -AMELIA:165:C03TUABXX:5:1101:12199:5354 - 1 565202 ACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTCATC >DDDDDDDB>DDEEEEFFFFFEEHHHEHEIIGJIIJIGJHGJIGIIGIJIIHHDHBHFJJJIIIIEJJIHJJJHHHJJJHFHHGAJJIGHHHHEDFFFCB@ 0 17:C>T -AMELIA:165:C03TUABXX:5:1101:13631:53360 - 1 565208 GCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTCATCATTAAT ?DBBDDDDDCDEEDEDBFFFHHHFHHHIIGIGIHGIIFDJIIJJIHHDGDGHJJJJJIIJJIHFJIHCHHJHGIEHHACEJJJIJIGHFHHHHDFFFFCC@ 0 23:C>T -AMELIA:165:C03TUABXX:5:1101:19409:61938 + 1 565218 CCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTCATCATTAATAATCATAATG CCCDDFFFHGHHHIJJIJJIJJJJJJJJJJIJJJJIIIJIIJGIIJJJJJJJJJJIIJJJJIJIJIJJJIHHHHHFFFFFFEDCEEEEDDEEDEDDDDDCD 0 67:C>T -AMELIA:165:C03TUABXX:5:1101:19186:17232 - 1 565230 TAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATA DDDEDDEEEEDFFFFFFHHHHHHHHDGAHFJJJJJJJJIJIHJJIHHGJJIJIGHHFJJJIHIIHEFIGJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC 0 45:C>T -AMELIA:165:C03TUABXX:5:1101:19104:7822 - 1 565234 AGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAAC DDDDDEDEEEFFFFFFHHHHE?HEHIJJJJJJJJJIHDIJJIHFJJJJIGHGEJJJIHJIHGFIIJJIJJIJJJJJJJJJJJJJJJJJHHHHHFFFFFBBC 0 49:C>T -AMELIA:165:C03TUABXX:5:1101:9877:53780 - 1 565235 GCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAACT DDDC?=A@C@@FFFFFFEHEEHEIDIIIHFIJJIHF@IIH@IHJJIHGEGBCJJIIHGGHCGIDIHGIIIJJJEJJJJJJJJJJJGGIFHHHHDDFDD@B@ 0 50:C>T -AMELIA:165:C03TUABXX:5:1101:5725:2316 - 1 565237 TATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAACTAG CCABCCC>@EC@DC?;;=.E=EAGHGIIGHFBDFBGFD@GDGIGBB:F??HEHCIGGGF?FCIIHEIGGIHGIHGHHGIHHGIGGGGGHHHDDFDBAD?@@ 0 52:C>T -AMELIA:165:C03TUABXX:5:1101:7045:12108 - 1 565237 TATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAACTAG DDDDEDEDDEFFFFFFFHHHHJIHGHIGIIJJIIFJIIGIGJJIIFBHFHJJJIHHIHHFJIIIEJJJIJJJJJIJJIJJIJJJJJJJHHHHHFFFFFCCC 0 52:C>T -AMELIA:165:C03TUABXX:5:1101:19955:7877 - 1 565238 ATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAACTAGG A=BA@A@AEDFDC@BD@7HHGBBGCBBIH@IGHFDHF?GDEHHF?:ABIHIIH@HFFB:C4<G>HFIHEHGHBBEBBEICGGFBIGIGGFHHBBAABBC@@ 0 53:C>T -AMELIA:165:C03TUABXX:5:1101:16597:18716 + 1 565243 CTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAACTAGGAATAG CCCFFFFFHHHHHJJJJJJJJIJGJJJJJIIGHIJIGGIHJJJFIJJIIJIJJIJJIJJJJIJJJJIIIHHGHHHGFFFFFEEEEEEEDDDDDDDDDDDDD 0 42:C>T -AMELIA:165:C03TUABXX:5:1101:2686:26086 - 1 565244 TTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTCATTATTAATAATCATAATGGCTATAGCAATAAAACTAGGAATAGC CDDEEEFFEEDBFFHHJJJJJJJJIHHFJIIHGDIJJJIGIGGJJJJIJJIIHIIJJIIHIJJJJJJIJJJJJIIGJJJJJJJJJJJJHHHGHFFFFFCCC 0 42:C>T,59:C>T -AMELIA:165:C03TUABXX:5:1101:14433:12289 + 1 565249 CAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAACTAGGAATAGCCCCCT @@CD=DD:CAFDFHIBGECGGFHGGACHGEGHE@FA<FG@<DCH>BF<DH=FH<4CHGG;7=FAECGG:@CHFEA=?CE>C@;C>AC>@?CCCCCA:CBBC 0 36:C>T -AMELIA:165:C03TUABXX:5:1101:5090:34555 + 1 565260 CCGGACAATGAACCATAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGA C@@FFFFDFHGHHIIIIJJJJIJIJIIIIIIIGIIGIIHJJJHGGHIJJJIJIJGHIIIIIIIJJIJJFGIDIIGFHHHFFFFFFEEDDDBDDDDDDDCCA 0 25:C>T -AMELIA:165:C03TUABXX:5:1101:11689:27070 + 1 565261 CGGACAATGAACCATAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGAG @@CFFFFDHBHFFEHGIJIIIJJJIHIJIFIJGIGGHHGGIIJJJIJIICGHJIIIGGIHGIIIG<GGGHGHIEGHIHHHE>BC>7=>ABCCCDDCDDDED 0 24:C>T -AMELIA:165:C03TUABXX:5:1101:8472:27484 - 1 565267 ATGAACCATAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAG DCCCCCCC@?CCCDC>;A;;@EDEDDCC?AA=EE@HHEIHCGHECGCBGGHEEIHHHD9B?EFD??9B4FD??B?GIIIIHF<C?C<GFBD?B;ADDD@@@ 0 82:C>T -AMELIA:165:C03TUABXX:5:1101:11947:56745 + 1 565272 CCATAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTT CCCFFFFFHHHHHJJJIJIJJJJJGJIIGGIIJJJIJJIJJJJJJIJJJJIJJIJJJIGIJJJIJJJJJIIDIIJHHHHHFFFFFFFEDDEDEDDCDDDCB 0 13:C>T -AMELIA:165:C03TUABXX:5:1101:10814:8192 + 1 565279 CAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAG CCCFFFFFHHHHHJJJIJJJJIJJJJJIJJJIJJIIIGGGJJJIJIJJIIJIHIIIIIJGIJIJHIJJJJJJJJJIHHHHHHFFFEFECDDD@ACDDDDDD 0 6:C>T -AMELIA:165:C03TUABXX:5:1101:15346:33163 + 1 565279 CAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAG ?=?;DDDD<;<+ACAC<E@A+AC?E4,<AD49?CC4:**@@393*C*:0:BDEIIADCEIECD<B8=B>DA@>=@==?CDD<;73?A;(;?@3;;;;3>9< 0 6:C>T -AMELIA:165:C03TUABXX:5:1101:11023:23471 + 1 565280 AATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGG CCCFFFFFHHHHHJJJJJJJJJJJJJJIJJIJJJIJJGJIJJJIJJJJJJJIIJJJIJJIJIIIJGIJJJJJJJJIHHHHHHHFFFFFDDDCBDDDDDDDD 0 5:C>T -AMELIA:165:C03TUABXX:5:1101:12077:41826 + 1 565284 CTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJHHHHHHFFFFFCEEEEDDDDDDDDD 0 1:C>T -AMELIA:165:C03TUABXX:5:1101:18199:60763 + 1 565294 ATACTCATCATTAATAATCATAATGGCTATAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCTCTGACAT CC@FFFFFHHHHFIJGEHGIJJIGIIJJJIGHIJJJIIJJJEHHIJIIIJJJJIIJJJJIJJJJJJJJDFGGHDHIHHHHHDEFBADDDDDDDDBDDDDD: 0 -AMELIA:165:C03TUABXX:5:1101:9413:29359 - 1 565299 CATCATTAATAATCATAATGGCTATAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCTCTGACATCCGGC CCCCACDC>A@:>>ACA>AA@A@@;CCCCCCCDC@;ACDC@C>9A<B@A@C8)FCECBFFFDCHFF@IGIHIIGFDHFCAHFBEIIIHHF<?DADDFF@@? 0 -AMELIA:165:C03TUABXX:5:1101:18992:48655 - 1 565301 TCATTAATAATCATAATGGCTATAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCTCTGACATCCGGCCT CDEEEEDEEEDDEDDDDDDEEEEDDDDDDDEEDDDDEEDDDDDDFHHHJJHIHJJIJJJJIGJJJJJJJJJIHJJJJJJIGEJJJJJJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:6359:38616 + 1 565321 TATAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAA @@@DD?=ADBC:AEBEBB?DF::F@GC@EFFE@B<DD<CDBGBF99??BB?B?/BF=B8;;@@C@A=B3).;)).78',3;8@BBA@B>A@DB>AB:>>>< 0 84:C>T -AMELIA:165:C03TUABXX:5:1101:11376:34877 - 1 565327 AATAAAACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTAG CDDDDC>C>DEDDDDDBDDDBCCC@@:DDCDDDDBAECACFFCFEEAFHHIHHHF@JIGF@EGCGJIGFJIJJIIGGJJHGHHCHEGGDHFHHFFEDFCC@ 0 22:C>T -AMELIA:165:C03TUABXX:5:1101:4273:24008 - 1 565332 AACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTAGCCCCC CA>3CC@C><D@BBDB@>A>C>CC@>:>ADDC>:>=5CDCC?CCEEC@?94EGGCCHC<=@?8IIGBIGIIGIHCGGIGGIIHEGHEDHDDCFAFFFF@@@ 0 27:C>T -AMELIA:165:C03TUABXX:5:1101:9665:13688 + 1 565337 GGAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTAGCCCCCATCTC CC@FFFFFHHHHHJJJJJJJJJIJIIJJJIJIIJFHIJJJJJJJJIJJJJJJIJJJJJJJJJHFFFFEDEEEDEDEDDDDDDDDDDDDDDDDDDDDDDDCD 0 68:C>T -AMELIA:165:C03TUABXX:5:1101:12720:2929 + 1 565344 CCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTAGCCCCCATCTCAATCATA @?@DDDDDFFDFDDBF@E<A:FH>?AF<FEFFBFBDG?GIFEI???DFFFBFIEIFEAFFAAE?CDBBBB;AC>B@A??@BBBABBBBBB@BABA4@AA:> 0 61:C>T -AMELIA:165:C03TUABXX:5:1101:15330:37509 + 1 565345 CCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTAGCCCCCATCTCAATCATAT CCCFFFFFHHHHHJJJJJJJJJIJJJHIJJJJJJJJJJJJJJIIJJJJJJJJJIJJJJJJHHHHHHFFFFFFFEEEEDDDDDDDDDDDDDDDDDDDDDDDE 0 60:C>T -AMELIA:165:C03TUABXX:5:1101:12886:48674 + 1 565348 CTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTAGCCCCCATCTCAATCATATACC @@@FFFFFFHFFHGFBGIIGGFHCFGHIIGGIEGIIGHHGHGGGGGDHIGIIGIGGGHHIGEFA.??CFECEFCCCCDCDDCCBDDDDDDCACCCCDDCDA 0 57:C>T -AMELIA:165:C03TUABXX:5:1101:1321:30663 - 1 565352 CACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTAGCCCCCATCTCAATCATATACCAAAT CCDDDDDDDDDDDCDDDDDDDBDDDCDDDB=DDDDEEEEEFFHHJIJJJJJIGIJJJIIIHJIGIHJJJJJHJJJJJJJJJJHIEJJJHHHHHDBFFFCCB 0 47:C>T -AMELIA:165:C03TUABXX:5:1101:7695:37930 - 1 565352 CACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTAGCCCCCATCTCAATCATATACCAAAT C@DDDDDDDDDDDDDDDDDDD@DDDCDDBB=DDDEEEEEEFFGHJJJJJJJJJJJJIIHHGJJHIGJJJJJHJJJJJJJJJJHJIJJJHHHHHFDFFFCCC 0 47:C>T -AMELIA:165:C03TUABXX:5:1101:1249:10236 - 1 565418 CAAAAACTAGCCCCCATCTCAATCATCTACCAAATCTCTCGCTCACTAAACGTGAGCCTTGTCCTCACTCTCTCAATCTTATCCATCATAGCAGGCAGTTG #######################################################D?DB?)C1*11*:)AFA2>BECDA:<+?::A24BAA::B8BAD=+: 0 11:G>A,29:T>C,40:C>G,47:A>G,55:T>C,60:C>G,65:T>C,74:A>C -AMELIA:165:C03TUABXX:5:1101:12677:58293 - 1 565442 ACATACCAAAGCTCTCCCTCACTAAACGTACGCCTTCGCCTCCCTCCCTCACGCTTATCCATCATAGCAGGCGGTTGAGGTGGATTAAACCAAACCCAGCT ###########################################################?B9*<4DB???1))1*+FBF<+3C4ABA;AD?;<2A=44+14 0 2:A>G,28:A>G,35:G>A,48:T>G,49:A>C,53:T>C,54:T>C,58:A>C,63:T>G,70:A>C,79:T>C,89:T>C,90:T>G,99:T>C -AMELIA:165:C03TUABXX:5:1101:9159:57992 + 1 565509 CAGGCAGTTGAGGTGGATTAAACCAAACCCAGCTACGCAAAATTTTGGCATACTCTTCAATTCCCCACTTAGGTGGATAAATAGAGGTCCAACGGAAAACC CCCFFFFFHHHHHHIJJJJIJJJJJJJJJJIJJJJJIIIJJ############################################################ 0 31:A>G,43:C>T,46:A>G,55:C>T,62:A>C,68:A>T,73:A>T,74:T>G,77:A>T,78:T>A,81:C>T,84:C>A,85:A>G,88:T>C,90:T>A,93:C>G,95:T>A,97:C>A,99:A>C -AMELIA:165:C03TUABXX:5:1101:2044:17116 - 1 565515 GTTGAGGTGGATTAAACCAAACCCAGCTACGCAAAATCTTAGCATACTCCTCAATTACCCACATAGGATGAATAATAGCAGTTCTACCGTACAACCCTAAC DCCAA<C>>@DDDDDDDDDDEDDFFDFHHHIIGHEEDGGJIGIHF@IIGB?>HGGF?EGIFGFIJIJJHGGJJIJIHGGIIHHBGFGIHFHHFDFFDF@@@ 0 25:C>T,75:A>G -AMELIA:165:C03TUABXX:5:1101:16159:18348 + 1 565525 ATTAAACCAAACCCAGCTACGCAAAATCTTAGCATACTCCTCAATTACCCACATAGGATGAATAATAGCAGTTCTACCGTACAACCCTAACATAACCATTC CCCFFFFFGHHHHJJJJJJJJJIJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJHHHHHHHFFFFFFDDDDDDDDDDDDDDDDDDDDDDD 0 15:A>G,65:C>T -AMELIA:165:C03TUABXX:5:1101:14266:28929 + 1 565525 ATTAAACCAAACCCAGCTACGCAAAATCTTAGCATACTCCTCAATTACCCACATAGGATGAATAATAGCAGTTCTACCGTACAACCCTAACATAACCATTC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJIJJJJJJJJJIJJJJJJJJJJJJJJIJJJJJJJHHHHHHHFFBCFFDCDDDDDDDDDDDDDDDDDDDDE 0 15:A>G,65:C>T -AMELIA:165:C03TUABXX:5:1101:10998:24784 - 1 565541 CTACGCAAAATCTTAGCATACTCCTCAATTACCCACATAGGATGAATAATAGCAGTTCTACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTA BBBDCDDDDECEECEFFEC?FHHHHHJIHD;IIIHJIGIIJJJHIIIIHGIIJJIIFDFDJJJIHIIHJJJJJJIJJHFJIIIGJIJJHHHHHDFFDFCB@ 0 51:C>T -AMELIA:165:C03TUABXX:5:1101:6612:25269 - 1 565541 CTACGCAAAATCTTAGCATACTCCTCAATTACCCACATAGGATGAATAATAGCAGTTCTACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTA BDBDDDDDCEEECCEFFFDBFGHHHHJJGGHIJIIJJJJJGJIEJJIJJIJJJJJIHHHDJJIJJJIIJJIIJIJIIHFJIIGJJJJJHGHHHFFFFFCC@ 0 51:C>T -AMELIA:165:C03TUABXX:5:1101:13214:44429 + 1 565545 GCAAAATCTTAGCATACTCCTCAATTACCCACATAGGATGAATAATAGCAGTTCTACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATATT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFFFEEEEEEEEDDEEFFEFE 0 45:C>T -AMELIA:165:C03TUABXX:5:1101:5654:2239 + 1 565546 CAAAATCTTAGCATACTCCTCAATTACCCACATAGGATGAATAATAGCAGTTCTACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATATTA CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJIJIJJJJIJJJJIIIJJJJJJJFGIJJJJJJJJJJJHHHHHHFFFFFFFEEEEEEEDEEEEEFED 0 44:C>T -AMELIA:165:C03TUABXX:5:1101:7009:59285 - 1 565554 TAGCATACTCCTCAATTACCCACATAGGATGAATAATAGCAGTTCTACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATATTATCCTAACT DEDEDC?DCECEEFFFFFHHHHHJJJJJJJIJJIJJJIJJJJJJIHFJJJJHIHGJJJJJJJJIIIJJJJJJJJJJJJJIJJJJJJJJHHHHHFFFFFBCC 0 64:C>T -AMELIA:165:C03TUABXX:5:1101:15667:17136 + 1 565564 CTCAATTACCCACATAGGATGAATAATAGCAGTTCTACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATATTATCCTAACTACTACCGCAT CCCFFFFFHHHHHJIJIJIJIJJIJIHIIIJIJJJJJIHIHIIJJJJJIJJJJIJJJJIIJJJJJJJJJIJIJJHHHHHHHHFFFFFFFEEEEEDDCDBDD 0 26:C>T -AMELIA:165:C03TUABXX:5:1101:20986:54325 + 1 565584 GAATAATAGCAGTTCTACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATATTATCCTAACTACTACCGCATTCCTACTACTCAACTTAAAC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJIIJIJJJJIJJJIJJJHJIJJJJIJJJJJJJIJJIJGHFFFFFEEEEEEDDDDDDDDDDD 0 6:C>T -AMELIA:165:C03TUABXX:5:1101:11116:17848 - 1 565589 ATAGCAGTTCTACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATATTATCCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAG DDDDDDDDDDBADDFFFFHHHHJIIJJJIHGJIIIIIIJJJIIGGIIJIIGGJJIJHGEJIIIIIGGIIHJIJJIIHIHHJHHFHEGHFHHHHEDDDFCC@ 0 99:C>T -AMELIA:165:C03TUABXX:5:1101:11782:53490 - 1 565590 TAGCAGTTCTACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATATTATCCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGC >>C>>@CCBBCCCDCBDDCAEEEGE@;@@7EG@=7>EGFAB98B8DBB<CEGEGDB@GHF?1?@BD@?1>GC3HHEGGGHHFCIGGFEA:HFDDBDDD<@? 0 100:C>T -AMELIA:165:C03TUABXX:5:1101:14433:27402 + 1 565591 AGCAGTTCTACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATATTATCCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCA @CCFFEFFHHHHHHJJJJJJJJJJJJJJJJIJJJJJJJJJJJIJJIJJJJJJJJJJJJJJJJJJIJJJJJJJIHHHHFFFFFFEEEEEEDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:11680:10437 - 1 565616 ATAACCATTCTTAATTTAACTATTTATATTATCCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCAC A@A:BBBA>@>@DDA;>>;ADA@DADECDDD@B?=7.A=7A5-IFGEF@ABF?9FB0D???9GBG@B?DD?FBCGEG?8ACAGAAA<E?AD:D@DDDD@?= 0 20:A>G -AMELIA:165:C03TUABXX:5:1101:15669:44253 + 1 565625 CTTAATTTAACTATTTATATTATCCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAA CCCFFFFFHHHHHJJJJJJJJIJJJJJJJJJJJIJJJJJJJJJIJJJJJJJJJJJJJJJJJIJJJJIJJJJJHHFEFEEEEEDDFDDDDDDDDDDDDDDDD 0 71:A>G -AMELIA:165:C03TUABXX:5:1101:5909:42099 - 1 565627 TAATTTAACTATTTATATTATCCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGC DDEDEEFFDEEEEEFEDDDDDDDDCEEEACB=FHHHJJIJIHHIIGIIIHGJJIHGDHFJIGHGBIHDIGEJJIJIIHAF:JIIGJIHDHHHHFFFFFCCC 0 31:A>G -AMELIA:165:C03TUABXX:5:1101:11313:64174 - 1 565631 TTAACTATTTATATTATCCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAAC EEFFDEEEEEFEDDDDDDDDCDDB;>BCFFHHGFHHGGCGGDIGIIIHGAHHFHCIIIHFBIIHHFBIIIHGHGCGFIHIIJJIHJIJHHHFHFFFFF@BB 0 35:A>G -AMELIA:165:C03TUABXX:5:1101:8927:15653 + 1 565642 TATTATCCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACAC CCCFFFFFHHGHHJJJJJJJJJJJJJJJIJGJJJJJIHIJJJJIJJJIGIJJJIIGHIJIIHHFHHHFFFFFDCDBDDDDDDDBABCCDDDCCCCDDDDCD 0 54:A>G -AMELIA:165:C03TUABXX:5:1101:18505:15917 - 1 565642 TATTATCCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACAC EDDDDDDDEDCDDCDDBDDEEEEEEDD?FECHGHHHIIIHHEHFJIIHHFJIHIHGJIIGHHGEIIHEJIIIGCJIGJJJIJJJJJJJHHHHHDFFFDBBB 0 46:A>G -AMELIA:165:C03TUABXX:5:1101:14349:44619 + 1 565646 ATCCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTT 1?<DDDBDDBDBDE@@D8<3AF9??A4?CE**9CA?BD?DDDDEED;DBDD@:;AC@;C47?3=??A6?83=>;>;--5<??:35::A::>34>>:>>;<9 0 50:A>G -AMELIA:165:C03TUABXX:5:1101:4627:38729 + 1 565648 CCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAA CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJIJJJJJJJJJJJHHHHHHFFDDEEDDDDDDDDDDDDDDDDDDDDDDDDDDDDC 0 48:A>G -AMELIA:165:C03TUABXX:5:1101:2646:22254 - 1 565649 CTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAAT DEEDDDADDDDDDDDDDDEFFEECEEEECFFFFDAHEHHIHGCJIHIHFJIIIIIIFJJIGHHJGHFJJJJJJIJJJJJJIIJJJJIJHHHFCFFFFFCC@ 0 53:A>G -AMELIA:165:C03TUABXX:5:1101:18300:5739 + 1 565660 CGCATTCCTACTACTCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAATTCCATCCACCC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJHHGHHFFFFFEEEEEEDDDDDDDDDDDDDEEEDEEDDDDDD 0 36:A>G -AMELIA:165:C03TUABXX:5:1101:14411:55186 + 1 565663 ATTCCTACTACTCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAATTCCATCCACCCTCC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJFHHHFFFFFFEEEEEEDDDDDDDDDEEEDEEDDDDDDDDD 0 33:A>G -AMELIA:165:C03TUABXX:5:1101:8926:20222 + 1 565673 CTCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAATTCCATCCACCCTCCTCTCCCTAGG CCCFFFFFHHHHHJJJJJIJJJJJJJJJJJJJJJJJJJIJIJJJJJJJJJJJJJJJJJJGHHHHHFFFFFDDDDCDEEEDEEDDDDDDDDDDDDDDDDDDC 0 23:A>G -AMELIA:165:C03TUABXX:5:1101:16872:45716 - 1 565680 TAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCCTG CCA:BADDDC?8DB>B?:DDDDDEFCDDBBCADEC?FFHHHHEJJJJJIIIIIJHFIIHGFDJJJJJJIIGIHHFGC:IIHFIGFHGIHHGHHFFFFFCCC 0 84:A>G -AMELIA:165:C03TUABXX:5:1101:15829:34863 - 1 565694 ACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGG >DC?DDEDCEDCDDDBDBDDC@DDEDDDBDDDCDEDDDEECCDCB9<2DDDDDDCCCDCCAB<5DDB?DA>C@FFFFHHHHJJJJJJJHHHHHFFFFFCCC 0 98:A>G -AMELIA:165:C03TUABXX:5:1101:10649:35099 - 1 565698 CCCTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACCCCCTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTT #####################################################################################EADDD<<0:ADDA<?? 0 57:A>C -AMELIA:165:C03TUABXX:5:1101:9796:8157 - 1 565708 ATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAAT DBDDDDC@DDEDDDDDDDDDEEEDEEDDDC@;?8DDDCACCA>DDCAB?2DDBADB9@>DDDDDDDDDDDDDDFJJJJJJJJJJJJJJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:6678:15930 + 1 565708 ATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAAT CCCFFFFFHHHHHJJJJJIJJJJJIJJIJJJJJJJJJJJJJJJJJJJJJGGIIJIJIJIJJJGEHHHHFFFECDEDBDBDDDDDDDBDDDDDDDCDDDDDC 0 -AMELIA:165:C03TUABXX:5:1101:5435:34952 - 1 565712 CGCACCTGAAACAAGCTAACATGACTAACACCCTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGC BCC@DDEDDDDDDDDDDEEDEDDDDC@5<2DDDDCDDCADDC<@<2DDDDDD@BCDDDDDDDDDBDDDCFJJJJIJJJIIJJJJJJJJHHHHHDFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:17278:52689 + 1 565726 GCTAACATGACTAACACCCTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAAGAAT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJIJJIJJJJJJJJJJJJJJJJJJJJJJJJHFFDDEEDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:18447:28878 + 1 565743 CCTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAAGAATTCACAAAAAACAATAGC @?;DD?DDFBFFHEGIFIIEC>D@FBA;H>391)?FH=FBG@FAF@GG9(;',=BC;63;5;5??>>:95<8>A:@>2<9?<>@:>CCACB?B99<A:C:A 0 -AMELIA:165:C03TUABXX:5:1101:18088:5598 - 1 565750 TCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAAGAATTCACAAAAAACAATAGCCTCATCA DDDCAB?2DDDADB@DCDDDDDDDDDDDDDDDDDDDEFFFEHJJJIJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJIJHHHHHFFFFFBCC 0 -AMELIA:165:C03TUABXX:5:1101:1438:10793 - 1 565753 ATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAAGAATTCACAAAAAACAATAGCCTCATCATCC ##############BC>43?8C?5B@<BB>8,3>;6BBEEG@EHDHFA>HFBAFBHFF;AHDHFB8AIIHDCE?:)1E@HF@IGGHFFF;AA:=?>DD=;= 0 -AMELIA:165:C03TUABXX:5:1101:20936:10496 - 1 565754 TCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAAGAATTCACAAAAAACAATAGCCTCATCATCCC 19<2DB?<B@>BADDDDDDDDB<DBBDDDBDDDDDDDFHHDJJIIJIGIGGIFJIHIIHG>IIHIIIIJIJIIIHGIIJJIIHGIGEJHGHGHFFFDDCB@ 0 -AMELIA:165:C03TUABXX:5:1101:4786:23803 + 1 565759 CCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAAGAATTCACAAAAAACAATAGCCTCATCATCCCCACCA CCCFFDEFHHHHGI>EFBGIEHDGIIGAHGGGIE6AFBBCDA<CECCA@7@;BBD@;;;>;;2>?8A@CCDA:ABBDDDDDDDDCC@?CCCA>CC<8<@>< 0 -AMELIA:165:C03TUABXX:5:1101:13159:16044 + 1 565762 CCTCTCCTTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAAGAATTCACAAAAAACAATAGCCTCATCATCCCCACCATCA CCCFFFFFHHHGHJJJJIJJJJJJJJJJJJJJIIJJJJJIIJJJGHHHHHFFFEEEEEDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 0 7:C>T -AMELIA:165:C03TUABXX:5:1101:5015:47230 - 1 565765 CTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAAGAATTCACAAAAAACAATAGCCTCATCATCCCCACCATCATAG DDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDEEFFFFHHHHHHJJJJJJJJJJJJJJIJJIJIIJJJJJJJJJJJJJJJJJIHHGHJHHFDHFFFFFCCB 0 -AMELIA:165:C03TUABXX:5:1101:15370:34088 - 1 565775 GGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAAGAATTCACAAAAAACAATAGCCTCATCATCCCCACCATCATAGCCACCATCAC DBDDDDDDDDDDDDB>DDDDDB<?B?BDEEDCCBFFFFEHHHHGIIIIIJIJJIGJJJJJIIIIIIGGCHGGIF?)FIIHFAIHIIJIFFHHHDFEFD@=@ 0 6:T>C -AMELIA:165:C03TUABXX:5:1101:17869:14860 + 1 565785 CGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAAGAATTCACAAAAAACAATAGCCTCATCATCCCCACCATCATAGCCACCATCACCCTCCTTAAC =1?7:DB:C1@00AEB1<BF9CBDGEFFEEDDFF9?D;D(?FFE)8=7@(('5?(=B3;@3;ABCA@(55=@>B5@55@A>A((+2<:94>@@B88?B### 0 84:T>C -AMELIA:165:C03TUABXX:5:1101:17890:52941 - 1 565808 TGGGCCATTATCGAAGAATTCACAAAAAACAATAGCCTCATCATCCCCACCATCATAGCCACCATCACCCTCCTTAACCTCTACTTCTACCTACGCCTAAT ?BC8:@CBA>8BCEDCC@AACDDDBCCCADEECCCDD@FDBHHEHEF=)IHF>GBB9AFD9HG<;?)GHGGIHIIIHFIHHFAEAFEECHGHHDFDFD@@? 0 8:G>A,39:T>C -AMELIA:165:C03TUABXX:5:1101:5341:37903 + 1 565820 GAAGAATTCACAAAAAACAATAGCCTCATCATCCCCACCATCATAGCCACCATCACCCTCCTTAACCTCTACTTCTACCTACGCCTAATCTACTCCACCTC CCCFFFFFHHHHHJJJJIJJEGGGIIIFIIIJJJJJGGGIIJIGIIJIJIJJJJIJIJCHIEHEEEFGFDFFFFDACECEDDDDDDBDDDDDCEDACDDDD 0 49:T>C,80:G>A -AMELIA:165:C03TUABXX:5:1101:6482:16845 - 1 565828 CACAAAAAACAATAGCCTCATCATCCCCACCATCATAGCCACCATCACCCTCCTTAACCTCTACTTCTACCTACGCCTAATCTACTCCACCTCAATCACAC DDDDDDDDDDDDDDDDDEDEDDDBDCDC@FFFEHHHHHIHGJHG@FBJIHGJIIHHFIHGIGHJIHHHFJIHHJJJJJJIIIGFIHIHFHHHHFFDDDBBB 0 28:G>A,59:T>C -AMELIA:165:C03TUABXX:5:1101:10045:17694 - 1 565828 CACAAAAAACAATAGCCTCATCATCCCCACCATCATAGCCACCATCACCCTCCTTAACCTCTACTTCTACCTACGCCTAATCTACTCCACCTCAATCACAC CBDDDDDC@DDCC@DDCECDDBB?BCBB;FEC>FHHHFHD=IGHEC5JJIGFGHFDFHF<F<BGDCF?1CGHFIHBGGHGHHFAG=BA<GHDGFDDDD@@@ 0 28:G>A,59:T>C -AMELIA:165:C03TUABXX:5:1101:5417:39176 - 1 565828 CACAAAAAACAATAGCCTCATCATCCCCACCATCATAGCCACCATCACCCTCCTTAACCTCTACTTCTACCTACGCCTAATCTACTCCACCTCAATCACAC ?BDDDCDDDDDDDDBDDCDDCB??DCA@7FEFEHHHHFHC=GHF@@5GGHFIHEHFBGB@GFBIIGFDDIGE?HEIGGHHEEC<FEGC<HFDDDDADD@?@ 0 28:G>A,59:T>C -AMELIA:165:C03TUABXX:5:1101:4715:40279 + 1 566461 CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTA @CCFFFDDFHFFFHDFBGHHIIJIJIGHGIDIGGEHGHHJIICHFDHEHEH@HIEHIJC@GBEDDCCCEDDCB=5:A>C@CDDDCDDDDDDDDDDDCACCC 0 -AMELIA:165:C03TUABXX:5:1101:1296:18675 + 1 566466 GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGA @@@DDFFFA8BFD>EDHFDHIHEFGIIHGGIIGBGGHGGIEHDHDCHIIBEIG@GAHGGIFHHGHHFB@B?@CCC@@CDCCB@ACD@CDCACB?:A@ACC> 0 -AMELIA:165:C03TUABXX:5:1101:1487:28026 + 1 566466 GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGA @?@DDDFFHHGHHJIEGIJICFCGHEGCHICHGGBGGGIGIJIIIIJHGIEHIFGHIIFIIF@E?EDBB;BC@C;>@CD=CBDDDCD@CCCCDCC>>A@A> 0 -AMELIA:165:C03TUABXX:5:1101:19758:60053 + 1 566466 GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGA @@?DBDDDHHDFD<DG<AA;FAECEGGGFHGIGGCHH??<??BFBHIIII4BAD?@BG@GIA>:A?@2?77>;C;>A@BCC<@:@:,(:>CC<?4AC:@<> 0 -AMELIA:165:C03TUABXX:5:1101:3067:40609 + 1 566468 TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGC CCCFFFFFGHHHHJJJJJJJJJJJJHIJJJJJJJJIJJJJJJJJJJJJJJJIJJJJIJJIHHHHGFFEFFFEEEDEDDDDDDDDDDDDDDDDDDDEEDEDD 0 -AMELIA:165:C03TUABXX:5:1101:11055:39672 + 1 566469 GACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCC B@CFFFFFHHHHHJJJJJJJIJJJJJJJIJJJIJJJJIIJJGJIJJIIIJJJIIJJJIEGHHHHHFDDFFFEEEDCCDDDDDDDDDDDDDDDDDEEDEDDD 0 -AMELIA:165:C03TUABXX:5:1101:3871:3936 + 1 566471 CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGA CCCFFFFFHHHHHJJJJJJJIJJJJJJJJJJJJJJIIJJGHIJIJJJJIJJJJJJIJJJIHHHHFFFFFFDEDEEDDDDDDDDDDDDDDDDDDEDEDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:12567:4042 + 1 566471 CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGA CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJIIJIJJJJJJJJJJJJJJIJJJJJGHHHFFFFFFEEEEEDDDDDDDDDDDDDDDDDEEDEDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:9555:12149 + 1 566471 CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGA CCCFFFFFHHHHHJJJJIJJJJJJJJJJJIJJIJJJJJJIJIJJIJJJJJIJJJJIGIJIHHHHEFFFFDEEAEECDDDDCDDDDDDDDDDDEEDEDDDD> 0 -AMELIA:165:C03TUABXX:5:1101:17991:12485 + 1 566471 CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGA @@CDDFFDHHFHFIGGIIDHIJIJIJIJJJGIEIHIIJJJJJJIIGIJIHGGIJJJIIIIGGHH@DCBBBCEEEDDDDDDDCDDDDDDDDDDEDDADDDDB 0 -AMELIA:165:C03TUABXX:5:1101:9449:14987 + 1 566471 CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTAGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGA @8;DDDDDF?FFFEIFEGEFGEGFE<EHIIE>BF?BFDFAF@FFFIIIF8:FFEFEFEEE3?A?=CD@?@DCCCA@?ABBBBABBB@BBBBBDDBABBB@# 0 60:G>A -AMELIA:165:C03TUABXX:5:1101:5387:17728 + 1 566471 CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGA CCCFFFFFHFHHGJJGHJIJJJIIGJJJIIJGIJGEGHHIIEGIHIIJJGEHIIJIJJJIHHEHCDE@CFEEEDCCBDDDDDDDDDDDDDCCDDDCCDDDD 0 -AMELIA:165:C03TUABXX:5:1101:11815:19737 + 1 566471 CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGA CCCFFFFFHHHHHJJJIJJJJJJJJJJIJJJIJJJIJJJIJJJJJIJJIJIJJJJJJJJJHHHHFFFFFECCEEEDDDDDDDDDDDDDDDDDEEDEDDDDC 0 -AMELIA:165:C03TUABXX:5:1101:16853:20244 + 1 566471 CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGA ?<8B==DBCDB<?:C??EDF::3A;?FE+AEEAC<D<CDF9<4??BD?DB<DADI=)77.7)?2@>?AD;>>AA>==>AA>A55>A?99AAA######### 0 -AMELIA:165:C03TUABXX:5:1101:9347:25795 + 1 566471 CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGA @C@FFFFFHFHHHJJJJJIJJIFGIIGJJIGIIJJIIJJIGIIGIJIIGIJIIJIIJJJJHHCHDFFDFDEECBEDBDDDCCCCDDDDDDDDEEDDC@BDD 0 -AMELIA:165:C03TUABXX:5:1101:2739:33305 + 1 566471 CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGA CCCFFFFFHHHHHJJJJJJJJJJJIJJJJJJIIJJJJJJIJJJJJJJJJJJJJJJJJJIJHHHHFFFFFFEEEEEDDDDDDDDDDDDDDDDDEEDEDDDDB 0 -AMELIA:165:C03TUABXX:5:1101:9574:42502 + 1 566471 CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGA CCCFFFFFHHHHHJJJJJJJJJIJJJJJIJJIIJJIJJJIIGIJIJJJJJJIJJJJJJJJHHFHDFFFFFEEEEEDDDDDDDCDDDDDDDDDEEDDCDDDD 0 -AMELIA:165:C03TUABXX:5:1101:15922:14294 + 1 566473 ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGC CCCFFFFFHHHHHJJJJIJJIJJJGIJJJJJJJJJJJJJIIIIJJJIJIJJJIIJJGIJHHHHHFFEFFFEEEEDDDDCDDDDDDDDDCDEEDCCDDBDDD 0 99:A>G -AMELIA:165:C03TUABXX:5:1101:19812:34860 + 1 566473 ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGC CCCFFFFFHHHHHJJJJJJJJJJJJIJJJJJJJJIJJJJGIJHIJ*?GHJJJJJJJJJJHHHHHFFFFFFEEEEDDDDDDDDDDDDDDDDEDDEDDDDDDD 0 99:A>G -AMELIA:165:C03TUABXX:5:1101:7701:55146 + 1 566474 TTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJGHHHHFFFFFEEEEDDDDDDDDDDDDDDDDEEDEDDDDDDDD 0 98:A>G -AMELIA:165:C03TUABXX:5:1101:21242:2052 + 1 566478 NTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGC #1=BBDDFDHGHHJGHGHHHIGGIJCHIGGHIIIGIE<GGGHDGEF<GIJCGCEGCACCEECAC@7?CACE;ACCCCCD?BDDDDDDCEDDD>>5<@?AB? 0 0:C>N,94:A>G -AMELIA:165:C03TUABXX:5:1101:10350:16546 + 1 566478 CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGC @C@DDDFDBHAFDDHDGIEG>?EHFEGIHCGEEGHGGGGEBFHAFBGEE@C@CGDHD7A=7==>?DBB@AC>6@@;??CAA<@CCED?DCB99<BBBCC?? 0 94:A>G -AMELIA:165:C03TUABXX:5:1101:9630:29859 + 1 566478 CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJIHHHHHFFFFFEDEEEDDDDDDDDDDDDEEDEDDDDDDDDDDDD 0 94:A>G -AMELIA:165:C03TUABXX:5:1101:14698:34931 + 1 566478 CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGC CCCFFFFFHHHHHJJJJJJJJJJJJJJJIIJJJJJJJJJJJJJIJJJJIJJJJJJJJJHHHHHFFFFFEDEEEDDDDDDDDDDDDEEDDDDDDDDDBDDDD 0 94:A>G -AMELIA:165:C03TUABXX:5:1101:10192:46446 + 1 566478 CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGC CCCFFFFFHHHHGIJIJJJJJJJJJIJJJIJJIIJJJJJJJJJJJJJIJJJJJJJJJHHHHHHFFFFFEDDEEDDDDDDDDDDDDEEDEDDDDBDDDDDDD 0 94:A>G -AMELIA:165:C03TUABXX:5:1101:3376:51520 + 1 566478 CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGC CCCFFFFFHHHHHJJJJIJJJJJJJJJJIJJJJJJJJJJJJJGJJJJJJJJJJJJJJJHHHGHFFFFFEDEEEDDDDDDDDDDDDEEDEDDDDDDDDDDDD 0 94:A>G -AMELIA:165:C03TUABXX:5:1101:15376:58671 + 1 566478 CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGC CCCFFFFFHHHHHJIJJJJJJJJJJJJJJIJJJJJJJIJJJJJGIJJJJJJIJJJJJGEHHHGFFFFFEDEEEDDDDDDDDDDDDEEDEDDDDDDDDDDDD 0 94:A>G -AMELIA:165:C03TUABXX:5:1101:7514:60520 + 1 566478 CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGC CCCFFFFFHHHHHIJJJJIJJJJJJJJIJJJJIJJJJJJJJIIIJJIJJJJJJJJJJJHHHFHFFFFFEDEEDDDDDDDDDDDDDEEDEDDDDDDDDDDDD 0 94:A>G -AMELIA:165:C03TUABXX:5:1101:12263:15006 + 1 566479 TACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCC ?<@DFA?DDD?;?;FEEEHHGDFD@G@FG<??FHEGGFGCGGA:FHECCFGA;7@DHEH>C7?C>B3;=>>C;A>>5>CBCCCCA;A><@B><B>5?<@BB 0 93:A>G -AMELIA:165:C03TUABXX:5:1101:4449:6178 + 1 566481 CAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAG CCCFFFFFGHHHHJIIJJJJJJJJJJJJJJJJJJJJJGJJJJIIJJJJJJJGEHCGIJHGHFHFFFFFEEEEEEDDDDDDDDEEDEDDDDDDDDDDCDDDB 0 91:A>G -AMELIA:165:C03TUABXX:5:1101:6062:6468 + 1 566481 CAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAG CCCFFFFFGHHHHJJJJJJIJJJJJJJJJJJJJJJJJJJIIGIJJJJJJJJJJJIIHDHHHHHFFFFFEEEEEEDDDDDDDDEEDEDDDDDDDDDDDDDD? 0 91:A>G -AMELIA:165:C03TUABXX:5:1101:15281:48072 + 1 566481 CAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAG CCCFFFFFFHHHHJJJJJIIJJJJJIIJJJJGIIIJCGIIFAHHIGIJJIGIFJHIIGHFHFHFFFFDEEEEEDDCDDDDCDEECECDDBBDDBDDDBBD? 0 91:A>G -AMELIA:165:C03TUABXX:5:1101:13923:50944 + 1 566481 CAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAG C@@FFDFFDHHDDHGGGEGJEGEHIIGIGEIEHIGIIIIGIGFHJJJIIJGIIJJJJGE?EHEEEDEFEDDECCCCDDDDDDDEC@ABB@B>@BDDBD?@? 0 91:A>G -AMELIA:165:C03TUABXX:5:1101:15367:46628 + 1 566483 AACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCC @CCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJIJJJIJIIJJIIJHHHHHFFFFFEEEEEDDDDDDEEDEDDDDDDDDDDDDDDDDD 0 89:A>G -AMELIA:165:C03TUABXX:5:1101:14145:49170 + 1 566485 CCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAG @@@DDDDDDFHHFEEFGGHGHCGEGCEB@HFIIJIEEHGAHGEGIIIIJGH@HFHGECHHHFHHFFFFFFEA@AECACDCDDCDDDDDDDCDDDDB<8??A 0 87:A>G -AMELIA:165:C03TUABXX:5:1101:15781:59661 + 1 566486 CACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGG @CCFFDEDHFHHGJJJGGGIIJIIIIDGGGIIIHBGGIJJIDGIJHIIBHHIIJEGHGGIHHHHHFFEFFEEDEDDDEEDDDDDDDDDDDC@DDDDDDBD? 0 86:A>G -AMELIA:165:C03TUABXX:5:1101:19827:48603 + 1 566488 CAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCA CCCFFFFFHHGHHJJIJJJJJJIJJJJJJJJJJJJJJJJIJJJJJIJIJIIJIJJJJJJJHHGHHFFFFFDEEEEEEDEDDDDDDDDDDDDDDDDDDDDDD 0 84:A>G -AMELIA:165:C03TUABXX:5:1101:3119:10012 + 1 566490 AAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAAC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJIJIJJJJJJJJJJJGIJJJJJJJJJJJJJHHHHHHFFFFEEEEEEEDDDDDDDDDDDDDDDDDDDDDDDD 0 82:A>G -AMELIA:165:C03TUABXX:5:1101:19971:11367 + 1 566494 CATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTC =8?+B:ABFDFAFBBE4:AF@CE?E?3C?C:FG@FF??BF@9BF@<=FCFAFFF;CD7).7;=AE>B?>>;@6;8>/38=2?9<<<<@BB<BB88@<<?:A 0 78:A>G -AMELIA:165:C03TUABXX:5:1101:1321:42339 + 1 566497 TGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAG CCCFFFFFHHHHHJJJJJIJJJJJIJJJJJJJJJJJIJIJJIJJJIJJJJJJJJIIGIJJHHHHFFFFFFEDDDDDDDDDDDDBDDDDDDDDDDDDDDDDD 0 75:A>G -AMELIA:165:C03TUABXX:5:1101:8440:11571 + 1 566506 ATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACC CCCFFFFFHHHHHJJJJJJJJJJJJJJIJHIJJIJJIJIJJJJJJJJJIIJJJJJJIIJHHHHFFDDDDCDDDDDDBDDDDDDDDDDDDDDDDCCCDBDD< 0 66:A>G -AMELIA:165:C03TUABXX:5:1101:8274:2189 - 1 566508 ACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCAC :DDEEDDDDBDDDDDDDDDDDDDDDDDDDDDDDCEEEEEEFFFFDBHHIJJJIJIHJJIJJJJJJJJJJJJJJJJJIJJJIHJJJJJIHHHHHFFFDF@BB 0 36:A>G -AMELIA:165:C03TUABXX:5:1101:2532:29186 - 1 566508 ACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCAC ####C:?@509><>>@A@@DDC@>CAC>CDCCCC@CCA>>>B;>BEECGEGE=HF6EGHDHEHGIIHFEGJIGCJIIIGJIHHFEHIIDBDFFDDDDFC@@ 0 36:A>G -AMELIA:165:C03TUABXX:5:1101:4214:54513 + 1 566509 CCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCACA @@@DFFBEFHHHHIBHHIDICEHIIIIIIGHIICDHGHGDHHIIIGHEHIIGIIIEGCHEEEEDDBCA@DD@BB??B?ABBBDCDDDDDCCACC@BD<>DD 0 63:A>G -AMELIA:165:C03TUABXX:5:1101:6848:10279 + 1 566518 CGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGCAACGACCACATCTACAACG @?8DADDAB?D:AEF@CGEHI<FEE9:B@@CCFF<:?B<B@;<FC<8B=F@;5;'::=?=265;15;=((,<9,>:?::;A(+28<<9>BB?>B>@@>>45 0 54:A>G,81:T>C -AMELIA:165:C03TUABXX:5:1101:15955:51501 + 1 566518 CGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCAAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCACATCTACAACG =:=DDDADHFHHHIGCFHHFHEGHF@GFFGIIJJIJHHIJJIIGIJIJGGII=CHHJIGHEHEFFD(>AAABABCDDCDCC@A:CB55<B<8>@CCDCDCB 0 52:G>A,54:A>G -AMELIA:165:C03TUABXX:5:1101:14157:59134 + 1 566521 CGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCACATCTACAACGTTA ?<7BA?DDBDAB8CFEB4B?:CF>F@BFEFIFFEIEBA9F9?GCFFFBFF'<';.;7;CE=CBBBD?B@@?BBBB;A@55>A?@B9B??>AB@:@>@B19? 0 51:A>G -AMELIA:165:C03TUABXX:5:1101:17801:43391 - 1 566524 ATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCG CCDADDDCDCDDDDDDDDCCDDDDCCCC?BD@CECECC@<FHFIGIGIIIIJIGEIIJIJIIGIGCGIJJIFIIJJIJGJIHHCIIHHFHHHFFFFFFCC@ 0 52:A>G -AMELIA:165:C03TUABXX:5:1101:18259:62242 - 1 566524 ATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCG @DDDDDDDDDDCCDCDDDCCDDDDCCDDBBDDDEEEDEDFFHEGJIJIJJJJJJJJJIGIIJJIGHJJHCJJJJJJJJJJIJIIIIIIFFFHFFFFFFCCC 0 52:A>G -AMELIA:165:C03TUABXX:5:1101:19001:60263 + 1 566536 CCTAGGCACTGCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCCATG ?@@?B3A3?BFF<CFDFF@GCFFGEECHCGEBFBFAF@EEEFF?FIIF..=CE==6?CCEFDD);;BBBB8;(5(5@BBBB3)(3<?(<+2?BBBBB?@A? 0 9:A>T,36:A>G -AMELIA:165:C03TUABXX:5:1101:9865:27038 + 1 566547 CTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATA @@@FFEDDHFHFBGFGEAFIEGIGGBEDGGDGEDGAGGG@BFFHFCDC;FHEHAHH=EEEDDDEDCCCCDC@<9C?CB<BDD@@ABB?>9>::>>A+>@CD 0 25:A>G -AMELIA:165:C03TUABXX:5:1101:19404:36205 + 1 566547 CTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATA @@@FFFFFBDFHFHIIEEB>BAF@EDAG?BEGIIBDF@FECGIGIHII@7CH=@DEC?EEBDDCCC>CAACB<<C?ADCABDCDD@B@ACDCEA>@::@AC 0 25:A>G -AMELIA:165:C03TUABXX:5:1101:14809:11362 + 1 566548 TCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAA @@@FFDFDAHHHHIIIBEGIIIGIIIFIGHIIIIIIHGIGBEHIIIIIIII=CHIIHHFDCDEEDCDCCCBBCDBDAABDDDDDDDDDDDDCDDDDEEEEC 0 24:A>G -AMELIA:165:C03TUABXX:5:1101:1665:17417 + 1 566557 CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCA C@CFFFFFFFGHHJJJJJIJJIIIJJIJIGGGIJJJJGDCHIGGIIIJJJHHGHFFFFFFED=CDDDDCDBBDCDBDDDDDDEEDE@CDDDCDCCCDDCDE 0 15:A>G -AMELIA:165:C03TUABXX:5:1101:13188:42545 - 1 566559 TTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATA CDDCDDDDDDDDCDDDDDDDCCCDFDEFEHEHHFJIIJJJJIHHFHIIGIIJIHGJIGJIIIIJIJJIHGIJJJJJJJJJJIJJJJJIHHGHHFFFFFCCC 0 87:A>G -AMELIA:165:C03TUABXX:5:1101:16854:35065 - 1 566568 CCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAATACCC DDDDDDDDDDDDDDDDEEEEECFFFFFHHHHHJJGHFIHDJIJJJIJJHJJJJJJJJJHHJJJJJJJJJJJJJJJJJJJIJJJJJJJIHHHHHFFFDBCCB 0 96:A>G -AMELIA:165:C03TUABXX:5:1101:7936:26745 + 1 566571 AGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAATACCCATC CCCFFFFFHHHHHJJJJJIJJJJJIJJJFGIIIIJJGJJJJJJJJJJJHIJJJJIIJHGHHFFFFEECEEEE@DECDDDDDDEDDDEEEDDFEFEDDDDD? 0 1:A>G -AMELIA:165:C03TUABXX:5:1101:20671:15670 - 1 566578 CCAGCCAGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCG <@CBBCC>@CC?BA@CCCCC?;<B:..CC=EEAEC;IGIEIHEFDIIIHGGGAEDHGB>BDGFAE?CCAHHB>GGEIIEIIGGGBE<CF?DD?DDDDB@@@ 0 -AMELIA:165:C03TUABXX:5:1101:3486:6811 + 1 566579 CAGCCAGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGG @@CFFFFFHDFHHJJJJJIJHGHIIEHIIJIHHGIIJJ@HHIIJGIJJJJIJIJIIIJHHHHHEDDEFFFFDECEECEDEDDECCDCDDDCDDDCDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:1557:53607 + 1 566584 AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACATCCCATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCT @@@DDFFFHGHHHIIIJIIIJJJJJIIJIIJJJJGJJJJIIGIIJJJIIJJIGIIGEHI@DHIJHGEFHHHFFFEDFDCDCEE@CCDDCDDDDDDDDDDDD 0 46:G>T -AMELIA:165:C03TUABXX:5:1101:12380:53549 + 1 566593 TCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACT @@CFFFDDHHGHHJJJJJIIJJIIJJIIJJJJHIIFIIIJJIJJJJJJIJHIIIJIJIIJJIJIJHHHHFHHFFFEFFEEECEEDDDDDDBACDCDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:12801:21084 + 1 566603 GACCACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCC @CCFFFDFHHHGDHGHHGIIIIIIIIGEGIIIGGIIIIIIIGIIGHIIIIIIIIGEGHGGIIIIIGIIIIIEHIGFEADABCECDCDCCDDDDDCCDCCDD 0 -AMELIA:165:C03TUABXX:5:1101:8127:62221 - 1 566603 GACCACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCC CDDDEDEEDDBDDCDEDDDFFFFHHHHJJJJJJJJIJJJJJJJJJJJJJJIJHJJJJJJJIGBJJJJIJJJJJHJJJJJJJJJJJJJJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:3713:64702 + 1 566604 ACCACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCC C@CFFFFFHHHHDIIJJIJJIJJIIEIGIJGIJJIIIIJDFGIIIJIIIJIJIIIIJJJIJIJJJJJJJIGJJJJHFFFDCCEEDDCCDDCCCCCC@CDCD 0 -AMELIA:165:C03TUABXX:5:1101:21177:4917 + 1 566606 CACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCT CCCFFFFFHAHHHIJIJJIIJJJJIIJJJ9DGHHIJIGIJIIIJJJJIJJIJJIDIIJJJIJJJJJGIIIJJIGHHFFDEEEEEDDDDDDDDDDDEEDDDC 0 -AMELIA:165:C03TUABXX:5:1101:1365:32118 + 1 566606 CACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCT CCCDFFFFHHHFHIIIJFIJIIGGFHGHCHHIJJJIIGGAFIIGHIIJGIGHIIJFHGHHIJIJJJJJJIIGCGH=BBAEDEEDCDDDDCCDDDACDDCD? 0 -AMELIA:165:C03TUABXX:5:1101:10046:60855 - 1 566606 CACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCT CDDDCDC@DDDBC?DBDDEEEDFFFGHHHIGIGIJJJIIGGIIIIJJIIHJIIIIGGIGBGHGHDEACIJHGJJJJIGJHIIIJIIIIFHHHHFFFFF@@C 0 -AMELIA:165:C03TUABXX:5:1101:10770:56986 + 1 566611 CTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAA ???DD>D8C>D:C?:CBEFEEBE?EEEICD<??DC99DB?DBDDADDEEA99?DBDIIIC8B8BA@C(5A<AC;3;@A@;A=A>A>AA>A>>A>=<89:AA 0 -AMELIA:165:C03TUABXX:5:1101:12510:36960 - 1 566615 AACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGG CCDDDDDDDDEDDDDEDEEEFFFDFEHHFFHHGJIJJIIIIJJIFHCHHF;JIGIGJIIJJIJIIIJJIJIIIJJIHJIGFJJJIIGJGHHHHFFDDD@CC 0 -AMELIA:165:C03TUABXX:5:1101:6017:37735 + 1 566626 CACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTCCCCCCGATA CCCFFFFFHHHHHIJJJHIJIJJJJJJJJJJJJJJGIJJIJJJJJJJJJJJJJJHIJIGIJJJJJJJJIJHHGHCEHFFFFCEEEEEEDDBDDDDDDDDDD 0 91:G>C -AMELIA:165:C03TUABXX:5:1101:15817:38927 - 1 566635 TGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTC DDDDDDDDEEDDDDDDDDDEDDEEDDEECC>DDDEDDDDDD@DDCDDDDDCDDDDFDDEEEEEEFFFHHHHJJJJJJHJJJJIJJJJJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:18441:4128 - 1 566636 GCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCC DDDDDDDEEDDDDDDDDDEDDEEDDDECA>DDDEDDDDDDDDDDDDDDDDDDDDFDDEEEEEEDFFFHHHHHJJJIGJJJIJJJJJJJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:20796:12973 - 1 566636 GCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCC DDDDDDDEEDDDDDDDDDEDDEDCDCDC>3DDDDCDDDDDBDDDDDDDDDCDDDDCDDECCEEDFFFHHHHHIGIHAJJJIJJJJJIJHHGHHFFFFF@@@ 0 -AMELIA:165:C03TUABXX:5:1101:4655:4005 - 1 566637 CATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCC CDDDDDEEDDDDCDDDDEDDEEDDCCDC?DDDEDDDDDB@DDDDDDDDDDDDDEDDECCEEEDDFFHHHHHHHJIGJIHIIJJJJJJJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:8140:28887 - 1 566637 CATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCC DDDDDDEEDDDDCDCCCDDDEEDCDADC>BDDDCDDDDDDBDDDDDDDDDDDDEDDEDDEEEDDFFHHHGHHGJIHJJJIJJGJIJJJHHGHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:19831:59011 - 1 566637 CATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCC CDDDDCCDCDDDCDACCEDDDECAB@>>3DDDC@DCB??7DDDDDDDDDDDCCBADCDCECCDCEFGGFFEFFEEBIHHBIGFIJIGJHHFDFFFFFF@@@ 0 -AMELIA:165:C03TUABXX:5:1101:21339:29116 - 1 566638 ATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCC DDDC@>ECCCDDDCCA>CC>@CC@:3:3DCCDCC@B@<7@BCCA9CDC@>5C@;@DCDDDCDDDFFEEHHHGHEHGFJIHJJIJJIHDHDFFHFFFFFCC@ 0 -AMELIA:165:C03TUABXX:5:1101:8359:55792 - 1 566638 ATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCC DDDDDEEDDDDDDDDDEDDEEDDDDCC9DDDEDDDDDB@DDDDDDDDDDDDDEDDEDDDDEDDDFFFHHHHHHJJJJJJJJJJJJJJJGHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:14271:5339 - 1 566639 TTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCG DDDDEEDDDDDDDCDEDDEEDDDDC@>DDDECDDDDB@DDDDDCDDDDDDDFCDEDDDDDDDDDEFFFFFEHHHIJJJJIIIJJIJJJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:15888:14151 - 1 566639 TTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCG >ABA:@>>4:9A?>A>:BB>:A:4+(>BA@::>>8<257B?ABBB@AA>@A@>:3AA>;;?3<>ADB??;?C=/EF;/;???:8FC>BFFFFF?@?DD@@? 0 -AMELIA:165:C03TUABXX:5:1101:13700:42519 + 1 566640 TTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGC @@@BDDFABF?CFBHID@H>@HEHGHHGIIBHCHGGDDEB?C:BF@FHGEGCHEA=CCCFGGGGCG@;CGDEHHCA5?B@CBBBBB8=>AA95><C:>?9> 0 -AMELIA:165:C03TUABXX:5:1101:12893:47362 - 1 566641 TGTAATAATCTTCTTCATAGTAATCCCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCG CC>EDDDCC?<DDDDDEEDDDC92(BC@@ADDBB?7DDDDBDCCCDDCDDCDDC@CDDDDDBDCD@CDBDBBDDDDCFGFJJIJGJJHAHGGGFDDFF@CC 0 0:A>G,76:A>C -AMELIA:165:C03TUABXX:5:1101:20798:46021 - 1 566646 TAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAAC >4@>ABBB@4@4>A@BBA:3?BB>>@A8<09;?<:?<B@>>::BDBBDBB@9920&5BBA@>@<@;B?==38<6A7/D@FDD@EFDBB>FDFFDA:?D??@ 0 -AMELIA:165:C03TUABXX:5:1101:15786:52087 - 1 566649 TCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAAC DDDDDEDDEEDDEEDCADDDEDDDDBDDDDDDDDDDDCDDDEDDEDDDDDDDDDDDEEEDDDDDDDDDDDJJJJJJJJJJJJJJJJJJHHHHHFFFFFCBC 0 -AMELIA:165:C03TUABXX:5:1101:9012:36976 + 1 566650 CTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACA CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJIJJIHHHFFFDDDDDDDDDBDDDDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:11518:28363 + 1 566653 CTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAA B@CFFFFFFHHHHJJJJJJJJJJJJJJHIIJJIIJJFGIJJJIIIJJHIIIIJJEGHJJIIJJHHFHHHFDDDDDDDDD@BDDCDDDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:8967:56720 - 1 566654 TTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAG CCDEDDDEEDC?DDDEDDDDDDDDDCDDDDDDDDDDEDDEDDDDDDDDDDDEEEDDDDBDDDDDDHJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:5894:61220 - 1 566655 TCATAGTAATACCCATCATAATCGGAGGCTTTGACAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGC CCEDADD@C@9DCAAACC?B?DDDDCDDCCCA:(A>>DCA>DCAB8D<BCDDCCBD9DDDDB@DFIIIIIIIGIIIGIGGGGHAGEIIHHDDGEEEDD@@? 0 67:G>A -AMELIA:165:C03TUABXX:5:1101:17037:25046 - 1 566658 TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTC CDDDDCA9DDCECDDDDDBDDDDDCDDDDDCDDCDEDDDCDBBBDDDEDEDDDDBDDDD@DHJJJJJJJJJJJJJJIIJJJJJJJIIHHHHHHFFFFFBB@ 0 -AMELIA:165:C03TUABXX:5:1101:10477:45945 - 1 566658 TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTC EDCDCCA9DDDDCCC?B?>DDCCBCCCDDDCCDCAA:CDCDDDBDDCDEEDDD@@DDBBBDHJIGIHHGAIGDIEAAEBIIIIIGHGFHFHHHDFFDD<?@ 0 -AMELIA:165:C03TUABXX:5:1101:4473:35890 - 1 566659 AGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCT CDDEDCADDDECDDDDDDDDDDDDDDDDDDDEDDEDCDDDDDDDDDEEEDDDDDDDDDDDHHJJIJJJJJJJJJJJJIJJJJJJJJJJHHGHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:17090:26464 - 1 566660 GTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTG DDEDCADDDEDDDDDDDDDDDDDDDDDDDDEDDEDCDDDDBDDDDEEEDDDDDDDDDDDFHJJJJJJJJJJIIJJJJJJJJJJJJIJJHHHGHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:17091:26488 - 1 566660 GTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCCG DDDCA9DDCDCDDDDDBDDDDDDDDDDCCDDADDDCDDDDDBDDDEEEDDDDBBDDDBDFHHIIGFJJJJJIJJIJIJJJJJIGIHJIHHHGHFFFFFC@C 0 1:T>C -AMELIA:165:C03TUABXX:5:1101:7764:54867 + 1 566660 GTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTG CBBFFFFFHHHHHJJJJJJJIJJJJJJJJIJJJJJJJJJJJJJJJJJIJJJIJJJIHIJJJJHFFDDDDDDD@BDDDDDDDDDDDDDDDDDDDDDDDDDDC 0 -AMELIA:165:C03TUABXX:5:1101:1416:62549 - 1 566660 GTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTG >DC@>3DDDEDDCDB?@DDDDDCDDDDDDDDADECDDDCB@DDDADDEDBDBBDDDDBBDGJJIHEJIIIIJIJIIJJIIJJJJJIIIHHHHHFFFFFB@C 0 -AMELIA:165:C03TUABXX:5:1101:17329:17612 - 1 566662 AATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGAC DDCADDDEDDDDDDBDDDDDDDDDDDDDEDDEDDDDDDDDDDDEEEDDDDBDDDDDDFHHJJJJJJJJJJJJJJJJJJJJJJJJJJIJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:5567:28191 - 1 566662 AATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGAC DCC>DDDDADDDDDDDDDDDDDDDDCDDEDDEDCDCDDDDDDDEEEDDDBBDDDDDDFHHJJJIJJIJJJJJJJJJJJJIJJIIIIHJHHHHHFFFFFB@C 0 -AMELIA:165:C03TUABXX:5:1101:8487:42357 - 1 566662 AATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGAC ACA9?DDC>DCBDB<DDDDDDDDDDCDDDCDEDCCCDD@BDDCDDCC?B95DBDDDDFGFIJGIJIGGEGJIJGGGIJJJIIIHJJJJHHHHGFFDFFB@@ 0 -AMELIA:165:C03TUABXX:5:1101:5674:3543 - 1 566668 CATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTAC DDCDDDDDDDDDDDDDDCCDCC@:CDCCDCDDDBBDDEEEDBDDDDDDDDDDEFHCHHIIGDIIIIIIIIIIIGIHFFIIIIIGGIIIHFHHFAFDDD@?@ 0 -AMELIA:165:C03TUABXX:5:1101:1744:25951 - 1 566668 CATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTAC DEDDDDDDBDDDDDDDDDDDDDEDDDDDDDDDDDDDDECC:DDD@DDDDDDDFFHHHGJIJJJJJJJJJJJJJJJJJJJJJIJJJJJJHHHHHEFFFFCBB 0 -AMELIA:165:C03TUABXX:5:1101:18331:41535 - 1 566668 CATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTAC DEDDDDDDBDDDDDDDDDDDDDEDDDDCDDDDB>DDDEEEDBDDDDDDDBDDFFHHHHJJJIJIGGHJJJJJJJJJJJJJJJJIJJJHHHHHHDFFFFBBB 0 -AMELIA:165:C03TUABXX:5:1101:16114:27825 + 1 566670 TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTACCT ???DDDDDF?1?CEEHGG>3AF=39EB>>FDAC@DF>DDB??@DH<HHDIGEEA<8?DCC;3,9;>>@<8?9@@A@AC???C@@>@>:@:::@CCC>>@CC 0 100:C>T -AMELIA:165:C03TUABXX:5:1101:5025:38875 + 1 566677 CGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTACCTCCCTCTC ?@7?DDA?DFDBFDGGC??AFHCHHHHIII=C<C9????)8)9DFGBA85@>3(9B8>ACCCB@BB@A3::@<@C@A344@>@>@ACC:4@AC::?8<C93 0 93:C>T -AMELIA:165:C03TUABXX:5:1101:6405:60500 + 1 566678 GGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTACCTCCCTCTCT CCCFFFFFHHHHHJJJJIJJIJIJJJJIIHGHIIIJJJHIJJIIJJJIJJJJJHEDDDFEECDDDDDDDDDDDDDCDDDDDDDEDDDDDDDDDDDDDDDDD 0 92:C>T -AMELIA:165:C03TUABXX:5:1101:19599:35795 - 1 566683 CTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTACCTCCCTCTCTCCTAC DDDDDDDEDDEDCDDDDDDDCDEEECDDDBDDDDDDDDDDDDDDDDDDEDFFHJJIIIIIJJGIIJIIHJJJJJJIIGEJIIHEAIGFHHHHHDFDFF@BB 0 13:C>T -AMELIA:165:C03TUABXX:5:1101:18910:7769 + 1 566689 CAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTACCTCCCTCTCTCCTACTCCTGC @@CDDD?DDHDHHIGHGIE@CEHHA:@EGAFGCD@F:@??8=@54@AGHIEBBC=>>AABAACC@CCACC@:;AC@CC@>AA><?9<?CC@ACCCC>CC@@ 0 81:C>T -AMELIA:165:C03TUABXX:5:1101:2203:16630 + 1 566689 CAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTACCTCCCTCTCTCCTACTCCTGC @?@DFFFFHAHHFGGH@ACCCHDHCC<FEGHDCGEE<:?FGEGFGGCHIIGDDC=@CCCABBCCCCCCCCCC;>ACCCCCACAABCCCCCCCCCCCCCCCC 0 81:C>T -AMELIA:165:C03TUABXX:5:1101:5950:18660 + 1 566692 CTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTACCTCCCTCTCTCCTACTCCTGCTCG CCCFFFFFHHHHHJJGGIJJJJGEHIIJJIIIJIJJJJJIGIIJJJJJHFFFEEEEDDDDDDDDDDDDDEDDDDDDDDDDDDDDDDCDDDDDDDDCDDCDB 0 78:C>T,99:T>C -AMELIA:165:C03TUABXX:5:1101:17385:5361 + 1 566694 GACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTACCTCCCTCTCTCCTACTCCTGCTCGCA CCCFFFFFHHHHHIHJJJJJJJHIJJJJJJIIIJJJJJGIJJJJJJHHFFFFEEDDDDDDCDDDDDDEDCDDD:C:ACDDDDCDCC3>:ACDCDCDCDDD> 0 76:C>T,97:T>C -AMELIA:165:C03TUABXX:5:1101:12490:62194 + 1 566708 ATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTACCTCCCTCTCTCCTAATCCTGCTCGCATCTGCTATAGTGGA @@@FFFFFFF>DHEGGGIBBHA@DGI>BFBD:AH8CE@=DA=C=7@@>DFD>;A>CAC@>>>5;??<99:5:A@@(++4:>3:>B@B?5>>>C>>A>CD@> 0 62:C>T,75:C>A,83:T>C -AMELIA:165:C03TUABXX:5:1101:16884:34094 - 1 566784 CCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGAC ######################??883=6>53;AAAA>@6)@;)7@=)?D?7;@-A;;=>DEDBBD?????B?D;DDD??<+@EE<EA3DDD;ADDDD??? 0 36:G>A,69:C>A,93:T>C,100:T>C -AMELIA:165:C03TUABXX:5:1101:16639:60510 + 1 566794 ATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCT @@@DBDDDBAH?BB?EEGGIG8?CFHDHHCHGGBBFEG9@F9B>DHGGBHII@>EGB.??EBBE:@;>CCB=@@B??B@?CBCC@9?@B@CC>@@?8:>>C 0 21:C>A,54:G>A -AMELIA:165:C03TUABXX:5:1101:14891:14079 + 1 566796 CTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTC CCCFFFFFHHHHGJJJJJJJJIJJJJJJIJJJJGIJJJJHHIJJIJJJJJJJJJJIJHHHFFFFFEEEDEDDDDDDDDDDDDDDBBDDDDDDDDDDDDDDE 0 19:C>A,52:G>A -AMELIA:165:C03TUABXX:5:1101:14167:29034 - 1 566799 CTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCC EDDDDDDDDDDDDDDDDDDDDDDDDDDDEEDDDDDECCA?FFHGJJIIGJJJJIIIJJIHGIHIHF?JHG<JJJJJJIJJIJIHGIJJHHHHHFFEDF@CB 0 51:G>A,84:C>A -AMELIA:165:C03TUABXX:5:1101:5966:50156 + 1 566817 CAGGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTC @@@DFFFFGHFBDGHIGIFFGHIIIIJJIJIGIJGEHFDIIGHHGGEFIIIHGIGHAHGCHHH=EBECEDCCBBEDCCAACCDCDDDDDDDDDDDDD<C@C 0 31:G>A,98:A>G -AMELIA:165:C03TUABXX:5:1101:20032:62435 + 1 566821 AACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCT CCCFFFFFHHHHGJIIJJIIJJJJJJJGIIJJJJJJJJJJJJJJJJJJJJJJJIJJJJJEGHGHFFFFFFEEEEEDEDDDDDDDDDDDDDDDD<CCDDDDD 0 27:G>A,94:A>G -AMELIA:165:C03TUABXX:5:1101:17388:16905 + 1 566826 GTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTACACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATC =?@?ADDDF4AADCAEEH=GIIE@CHAHH;BDGCBD@?DFEABB3B;(?;C)=/6;(;CE=;AEAC?C>@)(.6(;>5;;;3>;3::,95(5@@C:<C### 0 22:G>A,56:G>C,89:A>G -AMELIA:165:C03TUABXX:5:1101:5915:8663 - 1 566830 AACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTAG DCDDDCB?8DDDDDDDDDDDDCDDEDDCA?DDDD?=HHJJJIIIHJJJJJJIIHJJIIHJHGEJIHIGJHIHEFAIHGJJJJJJJJIJHHHHHFFFFFCCC 0 15:A>G,82:G>A -AMELIA:165:C03TUABXX:5:1101:1660:58353 - 1 566830 AACAGTCTGCCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTAG ABBB?20+&9<@?BBBB@>?>:>>;>@;;(=6>8;=:E@;IFIIFFF<6DDDCG@?DD?IFIIFEEIIIFFEGEFGHDFFAF?C:F9EFFDD?DD8DD@@@ 0 15:A>G,82:G>A,92:A>G -AMELIA:165:C03TUABXX:5:1101:21048:63246 + 1 567063 CTGGCATCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTT CCCFFFFFHHHHGJJJJJJJJGJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJHHFDDDDDDBDDDDDDDDDDDDEEEEEDCDDDBDDDDDDEEEEEDE 0 28:T>C,55:A>C -AMELIA:165:C03TUABXX:5:1101:9072:32585 + 1 567067 CATCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCG CCCFFFFFGHHHHJJJJJJJJJJJJJJJJJIJJJIJJJJJJJIJJJJGIJJJJHFDD@BD@BDBBBDDDDDDDEDDDDDDDDBBDDDDDDEEE@DDDDDDD 0 24:T>C,51:A>C -AMELIA:165:C03TUABXX:5:1101:6123:54504 - 1 567067 CATCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCG DEDC@EDDCCEDADDCCDB@<DDDC?>DC>D@A?5@@9DDD<BB<B<5DDFJJJJJJJJJJJJIHFJJJJJIGJJHHCIHGFFAIIIIHHHFHDFFFF@CB 0 49:A>C,76:T>C -AMELIA:165:C03TUABXX:5:1101:11110:59647 + 1 567067 CATCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCG CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJHFDDBDDBDDBDDDDDDDDDEEEEEDDDDDDDDDDDDEEEDEDEDDDD 0 24:T>C,51:A>C -AMELIA:165:C03TUABXX:5:1101:2231:62996 + 1 567067 CATCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCG ?1@DDDDDFDFA<EDHIIE?3CE<@GG>GH?:0B??FBB18B;?F<C(75@EHEE<?/''5</(888+8?83>C>>@:A(4(8?283@:4>>C######## 0 24:T>C,51:A>C -AMELIA:165:C03TUABXX:5:1101:7004:48986 + 1 567081 TAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTT @@CFFDDFHGHHFGIGDEHGIIIEHGEHIIGJJIIGIIJJJJFFBDDDB?B?BBDDDDCCDDDDDDCCD?BDDDDCDDEECCDDDDDBBBDDDDDDCCDAC 0 10:T>C,37:A>C -AMELIA:165:C03TUABXX:5:1101:13424:49164 - 1 567084 CAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTAT DDDBDDDCBCDDCDDBB<DC>DDC>?B>DB9DDDDDFFHHHHJJJJJIHJJJJIJGJGHHFJJIIGDJHIIIHIIJJJJJJJJJHHGCHHHHHFFFFFCCC 0 66:A>C,93:T>C -AMELIA:165:C03TUABXX:5:1101:13644:23889 - 1 567085 AGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATA DDDDDDDDDDDCDDBBBDC?DDDDDDBB@;DDDDDFFHHHHJJJJJIHJJJJJIIIIGFDJIHGGBJIGJJJJIJIJJJJJJJJIHFJHHHHHFFFFFCC@ 0 67:A>C,94:T>C -AMELIA:165:C03TUABXX:5:1101:2284:43018 - 1 567085 AGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATA <50DDAC?9DC>A<2<0B>3B?9&?DB@>B@BBBCBFHHEHHDEIEA4IIHGFB8BDDB7B;G??0DBHDCGIGFFEHAFBIIGBEAIDHFFGFDAFFCC@ 0 67:A>C,94:T>C -AMELIA:165:C03TUABXX:5:1101:20502:61471 - 1 567087 ACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATT >DDDDB?DDCBBA?5B@3DDDDDD><>5DDDDDFFFHHHHHJJJIFJJJJJJHIIJHDJJGIHFJIJIJIJJJJJJJJJJJIHGEJJJHHHHHFFFFFCCC 0 69:A>C,96:T>C -AMELIA:165:C03TUABXX:5:1101:18601:23704 + 1 567088 CCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTC @@@BFDAFFHHDHBFBHICGGHFHJE@FHGGIIIGGGHEECD?=AA=CD<=CDC>CDC@CC@BBBCCCDE@DDEDCDDDDDDDDDDDBDCCDCDDEDEEED 0 3:T>C,30:A>C -AMELIA:165:C03TUABXX:5:1101:12756:11610 + 1 567095 CTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJIJHHHHFFFFEEEEFEEDDDDDDDDDDEEEEEDEDDDDDB@BDDDDDDDDDDEEDEEEEEEDDDDE 0 23:A>C,95:C>T -AMELIA:165:C03TUABXX:5:1101:5244:13340 + 1 567095 CTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCT @@?DD?A?=DCFFIDFDBD9F?EBG@@?@@@BFCE;5(;5==;;BB;3>6.(5@;;;(:3>B;3@DE>D::9)5+)&44+2(<BA44:44>B>>:A:>A:4 0 23:A>C,95:C>T -AMELIA:165:C03TUABXX:5:1101:6298:22531 - 1 567107 TTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGG ABB?>DB<DDDDDDDDDDDDDDDDDDDEEEFFEFFFDAHHHEE@JJJJIIJJJJJJIJJIIHHC:JJJJJJJJJJJJJJJJJIJJJJIHFHHHFFFFFCCC 0 17:C>T,89:A>C -AMELIA:165:C03TUABXX:5:1101:19383:26755 - 1 567107 TTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGG A<D@BB>9DDDDDDDDDDDDDCDDDDDEEEFFDB>C@AHCA==)FIIIG@JJIIIIJIFIIHEFBFIGGHJJIIJIHFFGJIIIGCIIDDHHFFFFFFCCC 0 17:C>T,89:A>C -AMELIA:165:C03TUABXX:5:1101:5130:38014 - 1 567107 TTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGG ABBBD>95DBDB@?DDDCDDCCDDDDDEEEFDBBEDD?HHEC@7GGIJIHGHHIGGBJIIGGCCCJIIGIJJIJJIJHHEIIJIIHGGDHHDHDDFFF@@@ 0 17:C>T,89:A>C -AMELIA:165:C03TUABXX:5:1101:19357:14236 - 1 567110 TTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAAT ?<B>5DBDDDBDDDCDDDDDD@;BADEEECC>EB;FFEHECHJJJJIJIJJJJIIIIIHGGBJFJJJJHHFGGIIGIHEHHGGGHGEIHHHHGFFFFF@@@ 0 20:C>T,92:A>C -AMELIA:165:C03TUABXX:5:1101:3570:12721 + 1 567128 GGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAAC @@@DDD?AA:AFA?FD<E?AEAEFGIEBEGIICCCFGFDGB?@FF?DFICDF8BB=F;@C@=@FEIIIFFE>EEDBBDC?;@BBADBA@@A9>A>;;;AD: 0 62:C>T -AMELIA:165:C03TUABXX:5:1101:13405:52633 + 1 567128 GGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAAC ???B?DDDDDDD<EAEDE,ABEDCEEIEIEICEEFFIEDDI?@ADECDDDCCCEDIDEDIIEAEC@=CDIDACD?;;@?<>??;ADABAAA?A>AADDAA; 0 62:C>T -AMELIA:165:C03TUABXX:5:1101:5838:60820 + 1 567129 GAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAACT @C@FDDFFFGHFHGIJJIJJGGGIIIJJJFICGIJJJJIJJJJJJJIJHJJBCGGIJJJIJJIGEHHGIIHHHHFFDDCBDDDDECCDDDDDDDDEDEEDE 0 61:C>T -AMELIA:165:C03TUABXX:5:1101:5549:50505 - 1 567132 GCCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAACTTAC #?<>>AA>>5>>?<<=:>;>>3--(;;@;9=8A8ACA=C@CA@BCB<DEEDB?4D>D?**91?*:C1*BEECDDFECFFDA):BEAEC4BD>D:==DB<?? 0 42:C>T,100:A>G -AMELIA:165:C03TUABXX:5:1101:15172:2442 + 1 567133 CCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAACTTACT CCCFFFFFHGHHHCGIIIGJJJJIIJIGHIJJIJJIIIIIJJJJIJIHIIIIIJJJJJIJJJJJJJIIJJIHHHFFFFEEEEDEDDDDDDEEEEEDEEDDD 0 57:C>T -AMELIA:165:C03TUABXX:5:1101:8217:36810 + 1 567135 CCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAACTTACTAC CCCFFFFFHGHGGJGIIJJJJJJJJIJJJJJJJJFHFHIJIJJIIIJIHIJJJJJJIJJJJJIJCHIIJJIHGBDDFFEEEEEEDDDDEDEEDDEEDDDED 0 55:C>T -AMELIA:165:C03TUABXX:5:1101:16745:16615 - 1 567138 TTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCC DDEEDD@DB?AC>DDDDDDDDDDDCDFFHHHHD=JJIJJIIHGFDJIHGFJJJIGIHG?JJJJJJJJJJJJJIJJJJIJJJJJJGJIGFHHHHEFDDFCCB 0 48:C>T -AMELIA:165:C03TUABXX:5:1101:19666:31545 + 1 567138 TTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCC @C@DDFDFADHFHGEHIIIIHIEHHIIIGIICEFGIIIIEDB9BGCFIIGHDHIIIEGGIIIHIGIIGIHEHDFDFFFECECCCCDECDECEDCCCCCCC? 0 52:C>T -AMELIA:165:C03TUABXX:5:1101:13335:54298 - 1 567138 TTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCC CDEEDC?DDDCCCDDDDDDDDDDDDDFFHHIHD=JJJJJJJIJJJJJJJJJJJJIJHFHJJJJJJJJJJJJJJJJIJJJJJJJJJJJJHHHHHDFFDFCCB 0 48:C>T -AMELIA:165:C03TUABXX:5:1101:11949:59984 - 1 567139 TCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCG DEDDC<DDDC@:DDDDDDDDDDDDDDFHHHIG@JJJJJJJJJIJIIIIIJJJIGIHF?JJIJJJJJJJJJJJJJIIGJJJIJJJJJJIHHHHHFEDFFCCB 0 49:C>T -AMELIA:165:C03TUABXX:5:1101:19519:63698 - 1 567140 CTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGG DDC@8DDB>>5DDDDDDDDDDDDDDDFHHHE=JJJJJJJJJJJJGJIIJJJHGIHG?IJIJJIIJJJJJJJJJIJJJJJJIJIJJJIHHHHHHDDFFFCBB 0 50:C>T -AMELIA:165:C03TUABXX:5:1101:6477:5731 - 1 567197 CCAGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCT BBCBD>8BCCEFDCCDBDDBDECCCCCCCDFEDFEDD?EBIIJIJJJJJJIHHIGJIHCJJJJIIIIIFIIIIIIJIIIIIJJJJJJJHFHGHFFFFFCCC 0 56:G>A -AMELIA:165:C03TUABXX:5:1101:7871:54205 + 1 567216 CCCATATTGTAACTTACTACTCCGGAAAAAAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG CCCFFFFFHHHHHJJJIIIGHIJJIIIJJJJJJGGIAG;FHIGIFHAGGHGAG.7?CE??CD@>BC;@CEDCDCC@>C@CDCDCD@@CDD:A@CCC@<<<? 0 25:G>A -AMELIA:165:C03TUABXX:5:1101:16147:27246 + 1 567238 CCGGAAAAAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTA CCCFDFFFHGHHHJJJIGIJJIGJJIIJJJIFGGIJGHHIGHIJJJJJJIJJJIJJGIJJJJHHHHHF?DFFEDDEABDDDDDDDDDDDDDEEFEEEDDCC 0 1:G>C -AMELIA:165:C03TUABXX:5:1101:12910:34922 + 1 567241 GAAAAAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGA CCCFFFFFHHHHHJJJJJJIJJJJIIIJGGIJJEGHJIJJJJJJIJJJJJJJJFJJJJJJJJJJJEHHGFFFFFDCEEEDDDDDDDDDEFFEEEDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:7156:15040 + 1 567242 AAAAAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAA CCCFFDFFHHHHHJIJJJJJJJIIGHIBFHIEHHIJBHIIGIJIJJJJHGIIIJGIJJJIIJJI=AEHHHFFFDCCDEDDDDDDDCDEEFEFEDDCDDDDC 0 -AMELIA:165:C03TUABXX:5:1101:18044:25618 - 1 567242 AAAAAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAA BBDDDDDDCAEEDEEEFFFFEFFFHHGHHIJJGJJIIIJIJIJIIIIJJIJJJJIGJJGIJJJJIJJJIJIGJJJIHHFHHJJIIJJJHHHFHFFFFFCC@ 0 -AMELIA:165:C03TUABXX:5:1101:4436:29717 + 1 567243 AAAAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAAT CCCFFFFFHHHHHJJJJJJJJIJGIJEHIJJDHIJJJJJIJIJJJJJJJJJIJIJJJJIIJJJ=FHJGGHHHEDFDFFFDEDDDDDEFEEEEDDDEDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:4328:61899 - 1 567252 CCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGA CDDDDDDFEEEDDEEEEEEFFFFFHHHHHIJJJJJJJJJJJJJJJJJJJJJJJJJJIGIJJJJIJGJJIIGJJJJJJJJIJJJJJJJJHHHGHFFFFFCC@ 0 -AMELIA:165:C03TUABXX:5:1101:20640:63245 - 1 567258 GGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGACACACG DEFEDC@DDDDDDCBEEEFDFFFDHEHHFHHJIG@GGDIJIJJJIIIJJIGGGIJHHGHEGGBGHCDIGFJJIIGHJIJIIIJJGJIHHHHFHFDDDDB@@ 0 -AMELIA:165:C03TUABXX:5:1101:5739:63521 + 1 567287 ATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCA CCCFFFFFHHHHHJJJJJJEHIJIHHIIIGGIIGIIIIIIIJGJJIJIIJFHHIGIIIABHH@DHCHGJIFHFFDCCEEEFEEDDDDDDDDDDDDDCCDDD 0 -AMELIA:165:C03TUABXX:5:1101:10367:16002 - 1 567291 ATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGC DDDDDDDDDDDDDDDDDDDDDEEEEEEEFDECFFHGHHGHIHEJJJJJJJJJJJJJJJIJJIHGHHJJJJJJJJJJIGEJIHJJJJIHHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:13488:34447 + 1 567291 ATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGC @CCDFFFFGHGHHIJEEFHIIGHGHI@HIEHGHIJGGBFEIGGHIIHHIIHIIHGIIGBHHIIJGGIHFHFD>ACCCBEEDDDDBDDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:18290:8461 - 1 567296 CTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCC DDDDDDDDDDDDDDDDDDDDDDDDB@CE@CEFFFFECCHHHHHHHJIIGIHJJIHFGC=GGIIIIJJJJIHGD?JIJJIIGIHJIJJJHHHHDFFFFF@CC 0 -AMELIA:165:C03TUABXX:5:1101:17660:20544 - 1 567296 CTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCC 2@A><<BBCCCBB?B=CCCCCCA@A;AA@>DDDBEC?)??CAAFFG@@EDC;F@HFGGHEFEG@IGGAB@?D??GGAEFE?A<GHAICHFHD:?BBDD?<@ 0 -AMELIA:165:C03TUABXX:5:1101:2632:41586 - 1 567297 TTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCC DDDDDDDDDDDDDDDDDDDDDDDDCEEDEEEFFFFFFFFHHHHHHJIJIIJJJJIIHFHHJJJJJJJIJIHG?JJJJJJIHHJJJJJJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:19522:23017 + 1 567305 GTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCG BBCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJIIIJJIJIGJJJIJJJIJJJJJJJJJJJIJJIIJHHHHFFFDDDDDDDDDDDDDDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:18832:55892 - 1 567311 CGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAG DDDDDDDDDBCCDEEDEEEEDDDDDCCDEEEEDDDADDFDDDBBDDDDECEEDEEEC@;FFGJHGHGCJIIIJJIIIGJIJIIHJJJHFHHFHFFFFFC@C 0 -AMELIA:165:C03TUABXX:5:1101:16694:50299 + 1 567314 GTGAGCACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTAT CBCFFFFFHHHHHJJJJJJJJJJJIJJJJJJJJJJHIJJJJIJIJJJIJJJJJJJJJJJJJGJHHHFFFFFEDEEDEDDDDDDDDDDDDDDDDDDDDDCDD 0 -AMELIA:165:C03TUABXX:5:1101:4197:23414 + 1 567316 GAGCACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTT CCCFFFFFHGGHHJJJJIJJJHIJIEGIIIJJIFGIHJCHIIJIJJJIJIJJJJIJJJJJIJHHHFFFFFFFDCDAEDBCDDDDD@DDDDBDD<CC>CDDC 0 -AMELIA:165:C03TUABXX:5:1101:15375:49368 + 1 567316 GAGCACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTT ++:BDBDDFDFF>GHGH>H<3F>FGB@9EAH>?:;E8<@@9?6@F06D<CFH<8).8@=4@84=?DEEEE3@C;6;>;9;((5<;;;5>59<><>A+44>C 0 -AMELIA:165:C03TUABXX:5:1101:13522:33728 + 1 567317 AGCACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTA CCCFFFFFHHHHHJJJJJJJIJJJJJJJJJJJIJJJJJJJIJJJJJJJJJJJJJJIJJJJJJHHHHFFFFFFEEDEDDDDDDDDDDDDDDDDDDD>ADDED 0 -AMELIA:165:C03TUABXX:5:1101:9610:19001 + 1 567319 CACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGC CCCFFFFFHHHHHJJJJJHJJJIJJJJJJJIJJJJJJJJJJJIJJJJJJIJJJJJJIJJJJHHHHHHFFFFFDEDDDDDDDDDDDDDDDDDDD@CDEEEDD 0 -AMELIA:165:C03TUABXX:5:1101:12166:39897 + 1 567319 CACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJHHHHHHFFFFFDEDDDDDDDDDDDDDDDDDDDACEEEEDC 0 -AMELIA:165:C03TUABXX:5:1101:17945:6446 - 1 567321 CACCATATATTTACAGTACGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTG CCDEEEEEEEEEDDDDDDDEEEDDBBBDDDDBDDDDDDDCEEDDDDCA;DCBHHGHD=JJIGIIHHBEIIJGHFJJIHGJIIIHBIJJHHHHHFFFFDCCC 0 82:G>C -AMELIA:165:C03TUABXX:5:1101:15112:52346 + 1 567326 TATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCG CCCFFFFFHHHFHJJJJJJIJJJHIJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFEEDEDDDDDDDDDDDDCCEEEEDDDDDDDDB 0 -AMELIA:165:C03TUABXX:5:1101:20541:44901 - 1 567327 ATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC @AB>>@:CCCCC@>CCBCABDCCC@B?A<08>;>CFC>A@@A<B;>CDDEC?GHGIH@9EE@ADF@@7JIIHFIHHGHHEHGGIEGHGHHGHFDDDFFCC@ 0 -AMELIA:165:C03TUABXX:5:1101:5212:16261 - 1 567328 TATTTACAGTAGGAATAGACGTAGACACGCGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCC @>@::4>>A>:43@>?9827@:B?>5<0&<4::>@A@AA>9<?;8>>9??7@@EE=7?E@5@79F@1A:A@F6@FEF9<FFHEFE?<E8C?:<ADDAB1<@ 0 72:A>G -AMELIA:165:C03TUABXX:5:1101:11514:55763 + 1 567329 ATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCA CCCFFFFFHFHHHJJJJJJJIIJJJJJJJJJJJJIIJJJJJJJJJJJJJIJJJJJJJJJJJHHHHFFFFDEDDDDDDDDDDDDACEEEEDDDDDDDDBDDB 0 -AMELIA:165:C03TUABXX:5:1101:12131:50651 + 1 567336 GTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCA CB@FFFFFHHHHHHIJJJJJJJIJJIJJJJJJJJJJIJJIJIIJJJJJJJJJIJJJJJHHGHFFFDDDDDDDDDDD@CEEEEDDDDDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:21381:61755 - 1 567336 GTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCN 4>C>>DC??BB@CC>42(2<&?@A:43:@@:><2>>==BCA;(CCCCCB><A8BEDE=(/HGA7<IIGF@FIHFEFCDIIHC@?HECGFFDDFDBAA=11# 0 0:A>N -AMELIA:165:C03TUABXX:5:1101:14907:6681 - 1 567338 AGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACG DEEEEDDDDDDEDDDDDDBDDEDEDCDDDCA8DDDDDDCDADDDEEFDDDDDFFFFHHJIHFJJJJJJJIJJJJJJJJJJJJIHHJJJHHHHHDFDFDB@B 0 -AMELIA:165:C03TUABXX:5:1101:2445:40221 - 1 567342 ATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAG EDDDDDDEDDDDDDDDDEDEEDDDCC?2DDDDDDDDCDDDDEEDDD@DEEDDDFHHHBJJJJJJJIJJJJJJJJJJJJJIIJJJJIIHHFHFHFDFFFC@@ 0 -AMELIA:165:C03TUABXX:5:1101:13287:14727 + 1 567347 CGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATA CC@FFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHFDDDDDDD@CDFEEDDDDDDDDDDDDDDDDDDDDDDDDDDDDDC 0 -AMELIA:165:C03TUABXX:5:1101:5527:12142 - 1 567353 CACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAAT BBDDDDECEEDDDDCA<DDDDDDDEDDDDEEFDDDDDDDDDDDDFEBHJJJJJJIJJJJJIJJIJJJJJJJJJJJJJJHIGJHHJJJJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:16937:13965 - 1 567353 CACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAAT BDDDDDEDEEDDDDCA<DDDDDDDDDDDDEEDCDDDDDDDDBDDFEBHJJJJJIJJJJJJJJJJJJIGJIJJJJIHHHFJIJIHJJJJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:8695:22836 + 1 567355 CACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGA CCCFFFFFHHGHHIJJJJJJJIJJJIJJJJJJJIIIIJEGIJJIJJIJJJGEFDDEECDFEEFDDDDDDDDDDDDDDDDDDDDDD@DDDCDDDDEEDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:20005:46256 + 1 567355 CACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGA CCCFFFFFHHHHHJJJJJJJJJIJJJJJJIIIIJJJJJIJIIJJJJIIGGH?BCDEC@CDFEEDDDDCDCDBDDDBDDDDDDDDDBBDDDDDDDEEEDDDD 0 -AMELIA:165:C03TUABXX:5:1101:16207:48859 + 1 567355 CACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGA CCCFFFFFHHGHHJIJJJJJJJJJJIJJJJJJJJIIIIGIIJJIJJJJJJHDEDDEEACDEEEDDDDDDDDDDDDDDDDDDDDDDBDDDDCDDDEEEDDDD 0 -AMELIA:165:C03TUABXX:5:1101:15174:6243 + 1 567358 GAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCT @CCFFDABFFFHHIEHGIIHGHIIIHEIHCHEEIDGHIGGIGGIBHGJBHHFFF;>DDDCCECECDDA@DDBD?BCDCDDDB<@9A??CDCDDDCDCCDDD 0 -AMELIA:165:C03TUABXX:5:1101:6174:59924 + 1 567364 ATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCA CCCFFFFFHHHHHJJJJJJJIJJJJJJJJJJJJJJIJJJJJIHIJHHHAHFFFFFFFEEEEEDDDDDDDDDDDDDDDDDDDDDDDEEEDDDDDDEDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:7104:49457 - 1 567365 TTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAG CCA<2DB<D@ACD@DDDEEDDDDDDDDDDBDDDDDDFHIIGGIIJIIIGGGGIIIIGFHEJIIHDDCGEHFCJIJIGIIIGJGGHEJJGHHHFFFFFFC@C 0 -AMELIA:165:C03TUABXX:5:1101:1392:24669 + 1 567384 ATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATT CCCFFFFFHHHFFIIJGEFHGIEHHIGIHEIIIGIGGHGIJG;AGGFFHHFFFDBB>@BDCCCDDDCDDDC@CDEDDDDDDDCCDEDDDCDAC@D?CCCAC 0 -AMELIA:165:C03TUABXX:5:1101:4437:34241 + 1 567386 CATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCA CCCFFFFFHHHHHJJJJJIJIIJJJIFHIIJHIJJJJJJIJJIIJJHHHHFFFDDDDDDDDDDEEEDDDDDDEDDDDDDDDDDEDDDDDDDDDDDDDDDDC 0 99:T>C -AMELIA:165:C03TUABXX:5:1101:3750:14375 + 1 567389 CGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCT CCCFFFFFHHGHHJJJJJIIIGIGHIJIGJJECHIEGIHIJJJJJJGHHHFBEADEEDDDEDDD@CACDECDDDDDDACCDDDDDDDDBCCDCCCDDDEEC 0 96:T>C,99:T>C -AMELIA:165:C03TUABXX:5:1101:20985:15268 + 1 567390 GCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTT CCCFFFFFHHHHHJJJIJJJJJFIGIJIJJJJIJJJJIJGIIJJJJJHHHFFDDDEEEDEEEDCDDDDEDDDDDDDDDDEDDDDDDDDDDDD@CDDDEEDD 0 95:T>C,98:T>C -AMELIA:165:C03TUABXX:5:1101:17084:41086 + 1 567393 ATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCT @CCFFFFFHHHHHJJJJJJGHIJJJJJJJJJJJJJJJJJJIJJJJJHGFFFFEEEEEEEDDDDDDEDDDDDDDDDCDDDDDDDDDDDDDDDDDDEEDDDDD 0 92:T>C,95:T>C -AMELIA:165:C03TUABXX:5:1101:2695:8781 - 1 567408 AAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGG DDCDECDDDDCDCCBBBDDDEECCB=FHCHIJJJIIIGGIIHIIGGFHBHGGHHCGIGJGIHFCGGJJJJIJHHHBHFGHG@GJIFIIDHHFHDAFFF@CC 0 20:T>C,23:T>C -AMELIA:165:C03TUABXX:5:1101:11387:8636 + 1 567409 AAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGC @@@4BDDD<D:A<<@4?C6EFF3BD2CDFF6DFA9FGF>B<DE>D>D9?CFEFE>FE=C>E>AEEE;;1==BD:>;>>A@ACAA>A@BBBBB?,5(,5>?B 0 76:T>C,79:T>C -AMELIA:165:C03TUABXX:5:1101:4065:8678 - 1 567410 AGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCC CCDCCC:CCCC<8;?@??>;>6?59??HFHE>IIIIHEDGIIIIGGGGCGDGGGDHEIFGIGHCBHFD?GCHCHGGAEFAHGGCC<;A3DHFF;DDDD@@@ 0 22:T>C,25:T>C -AMELIA:165:C03TUABXX:5:1101:16303:52642 - 1 567411 GTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCT DEDDDDDDDDDBDDDDDDEEADDFEFFHHHHHJJJJJJJJJJJJIJJJIJJIJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJIHEHHHHHFFFFFCC@ 0 23:T>C,26:T>C -AMELIA:165:C03TUABXX:5:1101:21044:23461 + 1 567413 ATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGA CCCFFFFFHGHHHJJJJJJJJJJJJJJJJJJJIIFIIIIJHIEHHIJJJJJJIHIJJJJIJIEHHHHHHFFFFFFEEEEEEEDDDDDDDBBDDDDDDDDBC 0 72:T>C,75:T>C -AMELIA:165:C03TUABXX:5:1101:18907:56221 + 1 567413 ATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGA CCCFFFFFHHHHHJJJIJJJJJJJJJJJJJJJJJIIJJJJIGIJJJJJJJJJJIJJJIJJJJIJHHHHHCFFFFFEEEEEEEDDDDDDDDDDDCDDDDDDD 0 72:T>C,75:T>C -AMELIA:165:C03TUABXX:5:1101:12974:46238 + 1 567417 AGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGG ?@@DDDDDHHDDFHGGHHEGGIEFGCFHI@GGDBGHEF@FHHGDDB8BFB=BC7CH7=;7@7=EC?3=?C>;;@CE@@;;;>ACBBDDBDD?BACBCC@A< 0 68:T>C,71:T>C -AMELIA:165:C03TUABXX:5:1101:20635:7983 + 1 567420 TGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCAT CCCFFFFFHHHHHJJJJJJJJJJJJJIJJIJJJGIJJJJJJJIIJJGIJJJJJJJIIJIIJIGHHHHHHHFFFFFFECEEEDDDBDCDDDDDDDDDDDDDD 0 65:T>C,68:T>C -AMELIA:165:C03TUABXX:5:1101:12126:13040 + 1 567423 CTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGT CCCFFFFFHHHHHJJJJGIJJJJJIJJJJIJJJJJJIJJJJJJJJIJJJJIJJJJJGIGDHHHIJJHHHHHHHFFFFFDDDDDDDDDDDDDDDDDDDDDDD 0 62:T>C,65:T>C -AMELIA:165:C03TUABXX:5:1101:18388:7478 - 1 567433 TCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAAC BDDDDDDDDDEEEEEEEDEEEEEFFEFFHHHHHJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJIGDJJJJJJJIJJJJJIIJJJJJIIHHHHHFFFFFCBC 0 45:T>C,48:T>C -AMELIA:165:C03TUABXX:5:1101:14458:16696 - 1 567433 TCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAAC BDDCDDDDDDEEEEEEEFEEDEEFFDFFHHHHHJIJJJJJJJJJJJJJJIJJJIJJJJIJIJJIFBIJJJJJJJJIIJJJIJJJIJIJHHHHHFFFFFCCC 0 45:T>C,48:T>C -AMELIA:165:C03TUABXX:5:1101:13540:8867 - 1 567437 CGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCAT DDDDDDEEFEEEEFEDDEEEEEFFFFFHHHHHJJJJJJJJJJJIHAJJJJJJJJJJJIIGGBJJJIJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC 0 49:T>C,52:T>C -AMELIA:165:C03TUABXX:5:1101:13723:41463 - 1 567438 GGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATC ADDDDEEEEEEEFEDDDEEEEEFFFFFHHHHGJJIGIIIJJJJJIJJJJJJJJJIHGGGGDJJJJJJJJJIJIGHDJJJJJJJJJJJJHGHHHFDFFFCC@ 0 50:T>C,53:T>C -AMELIA:165:C03TUABXX:5:1101:16226:60657 - 1 567443 CAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAG DEEEEEEEEDDDDDDEEEEEFFEEDCEHGHIGIJIIGJIIIIIJJJJIIJIHGEGDJJJJIJJGGIJJJHGJIJJJHJJJJJJJIIJJHHHHHFFFDFCCC 0 55:T>C,58:T>C -AMELIA:165:C03TUABXX:5:1101:1952:58309 + 1 567449 GAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCG @@@DDFFFHHHHHJIJGFHIIIJIIHIIJEHIJBHHIIIEIJGIJJJJIIJJIHIAHBFFGIHGIJJJIGGGIHECHHGFFFFFFEEEEEEDDDDDDDCCB 0 36:T>C,39:T>C -AMELIA:165:C03TUABXX:5:1101:1255:6028 + 1 567450 AAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGT CC@FFFFFGFHHGIJIIIIGIIGJIJJIIIIJEHIJEHIIIJIJIJJJJJJIGGGIFGHGHIIIJIGDGIGIHEEEHHGHFFFFFDEEEEEDDDDDCCDD? 0 35:T>C,38:T>C -AMELIA:165:C03TUABXX:5:1101:21056:38862 + 1 567450 AAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGT @@CFFFFFDAHDHJ;EGGEIGIBEFIIJJGGGFAGHIIGHIJHGHEIIJJJIJJJJ<DHGHEECGHEECHIIHEECHFHHFFE@DCCECCCDCCCD5@ABB 0 35:T>C,38:T>C -AMELIA:165:C03TUABXX:5:1101:2958:62133 + 1 567450 AAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGT CCCFFFFFGGHHHIJGHIIJIJIIIJJJIDHIGIIJIEHGIJGIIJIJIJJJFHIIFHHIIJJJJJJJIIJJHHGEHHHHFFFFFEEDEEEDDDDDDDDDB 0 35:T>C,38:T>C -AMELIA:165:C03TUABXX:5:1101:18475:17325 + 1 567452 ATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTAC CCCFFFFFHHHHHJIJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJIJJJJHJJJHIJJJJJIJJJJJJJJJHIHHHHHHFFFFFFEEEEEEDDDDDDDDDD 0 33:T>C,36:T>C -AMELIA:165:C03TUABXX:5:1101:8129:61077 + 1 567452 ATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTAC CCCFFFFFHHHHHJIJJJJJJJJJJJJIJJHJJJJJIJJJIJJJJGJJJJHJJJDHIJJJJJJJJJJIJJJHIHHGHHGEFFFFFEEDEEEDDDDDDDDDD 0 33:T>C,36:T>C -AMELIA:165:C03TUABXX:5:1101:19947:16236 + 1 567455 ATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTAC @@CFFFFDHHHFHHJJJJJJJJJIJIJEIIIJJJJJJJJIJJGGGIGGHIJHHJJJIJGIHIJIGHIIGEGIIFHHHHFFFFFFEEEEDEDDDDDDDDDED 0 30:T>C,33:T>C -AMELIA:165:C03TUABXX:5:1101:11385:6541 - 1 567457 CTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTTGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACAC ######A;=>;AA?6.((6)?:@?7)=>A7773@C@7)=@@=A7*BB?8909BBHB??:*:1*9:*?EAA4@A>HCEBED??<9DC<2?3CDCA:A44111 0 50:G>T,69:T>C,72:T>C -AMELIA:165:C03TUABXX:5:1101:6148:29232 + 1 567457 CTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACAC CCCFFFFFHHHHHJJJIJJJJJJJJHJJJJJJJJJJJJJJJJJJJIJJJHIJJJJJJJJJJJJJJJHIJJIJJHHHHHFFFFFFEEEEEEEDDDDDDEDDD 0 28:T>C,31:T>C -AMELIA:165:C03TUABXX:5:1101:17519:57571 + 1 567457 CTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACAC @CCFFDDDHHFHHGEIGGGGDFHIJ>CDHIGDFHGEGGGIGHHGG:BFD?BGBGFBFH>FFICGGI@CDHHDGHHAEE>CCCDECCACECED@?BBDDCCA 0 28:T>C,31:T>C -AMELIA:165:C03TUABXX:5:1101:16105:60498 + 1 567458 TGCTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACG CCCFFFFFHHHHHJJJJJJJJJJJHIJIJJIIJJFHJJJIIJJIIJJJBGHJJJJJJJJJIJJJJIIJIJJJJHHHHHFFFFFFEEEEEEEDDDDDEDDDD 0 27:T>C,30:T>C -AMELIA:165:C03TUABXX:5:1101:3964:5095 - 1 567460 CTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGAC DDDDDDDDDDDDDDDDDEEEEFFFFEFHHHHHJJIIEF=JJIJJJJJJHJJJIIJJJJJJJJJJIIJJJJJIIJIJJIJIJIIHGJJIHHHHHFDFDFB@@ 0 72:T>C,75:T>C -AMELIA:165:C03TUABXX:5:1101:21195:37349 - 1 567460 CTGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGAC DDDDCBCCDDDCDDCCECEEEFFFCCFGFHHFGJJIIGHJJJJJJIIHBHIJJIJJJJJGJIIJJJJIGIIIHGIJIHHFIHIGGDBFHHHDHDBDDD@?@ 0 72:T>C,75:T>C -AMELIA:165:C03TUABXX:5:1101:11454:26095 + 1 567461 TGCAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACA CCCFFFFFHHHHHJJJJJJJJHIJJJJJIJJIJJJJJJJJJHGIJGHIJJJJJJJJJJJJJJGIIJJJJJJJJHHHHHHFFFFFFEEEDDDDDEDDDDDDD 0 24:T>C,27:T>C -AMELIA:165:C03TUABXX:5:1101:20953:62923 + 1 567465 GTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTA @CCFFFFFGHDFHGIGHIFGIGFBFHIIJIEGIHGF;CGIEH<?GDDGHGAHGHJHHHBGHE>CHIJGDECHECAEHHHB>CD@ABBCD@CCD<?BDBBB< 0 20:T>C,23:T>C -AMELIA:165:C03TUABXX:5:1101:1416:42521 - 1 567476 CCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAG DDDDDDEDDDDDDDDDDDDCCB@DFDFFHGHHIJIJJHJJJJJJIJJJJJJJJJIGGJGIIIHHJJIGGGGHJGGHJIHHFIHGHGJJHFHHHFFFFFCCC 0 88:T>C,91:T>C -AMELIA:165:C03TUABXX:5:1101:6008:48771 - 1 567476 CCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAG DDDDDDDDDDDDDDDDDDDDDA@DFDFFHHHHJJIIHHJIJJIJJJJJJJJJJJJIHJIJIIIIJJIHHJJJJJJIJIHIIJIIIHJJHHHHHFFFFFCCC 0 88:T>C,91:T>C -AMELIA:165:C03TUABXX:5:1101:13720:54391 + 1 567483 TTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTT ???DDDDBDD>BDE4<<ACF;1AF<1A?<19???DAD<?D:*??DD>?DI?8DEIIICB@<88./85.@CDC?AA54?@@8?>??################ 0 2:T>C,5:T>C,95:C>T -AMELIA:165:C03TUABXX:5:1101:14126:5482 - 1 567487 TCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCAC DDDDDDDDDDDCDDDDDEDDDFFFFHHHHHHJIJJJIJJJJJJJHGJIJJIHDJIJIIIJIJJJIJJHIHJIHHHJIJIJJHJIIJJJHHHFHDFFFFBBB 0 9:C>T,99:T>C -AMELIA:165:C03TUABXX:5:1101:17997:2449 + 1 567488 CTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACT CCCFFFFFHHHHHJIJJJHIJJJIJGIJJJIIJJJIIJJJIIJJJJJJJJJJJJJJJJCHJJIIJJJJJJHHFFDDECDDEDDDDDDDDDDDDDDDDDDDD 0 0:T>C,90:C>T -AMELIA:165:C03TUABXX:5:1101:1302:27930 + 1 567488 CTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACT @@@DBDDDD>DFHHHHGEAFEH>AADGHIIIIGIIFEGEFFHGGHIGIEDGIIIEHGEHDFF=CHHIIGI=EDECCCCABDCBBCA8@CCCACCCCCCCC@ 0 0:T>C,90:C>T -AMELIA:165:C03TUABXX:5:1101:16014:59652 + 1 567492 CTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGT CCCFFFFFHHHHHJGHJJJJJJJJJJJIJJJIGIJJJJJJJJJJJJJJJJJJJJJJJJHIJJIIJJGJHHFFECDDEDDDDDDDDDDDDDDDDDDDDDDDE 0 86:C>T -AMELIA:165:C03TUABXX:5:1101:20305:32210 + 1 567494 TTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCC @@@DDFFFHHHHFGGJJJGIGDFHGIDGG?FIGGGIJJGHJICGEHIGHJIJGGGIIGHIIJGIIIGFFFDEDDDCBADDDDDDCDDDDDDAACDDDDEED 0 84:C>T -AMELIA:165:C03TUABXX:5:1101:9775:3352 + 1 567499 CCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCA @CCDDFFFHHHHHJIIIJJJJJIJGJJJJJJJJJJJIIIJJJIIIIJIJIJHIJJJJJJJJJJHHHHFFFFDEEDDDDDDDDDDDDDDDDDDDEEEDDDDD 0 79:C>T -AMELIA:165:C03TUABXX:5:1101:4951:56096 + 1 567499 CCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCA CCCFFFFDHHHHHJJJJJJJJJJJIIJJJJJJIJJJJJIJJJJJJJJJJJJHGIJJJJJJJJJHHHHFFFFDEEDDDDDDDDDDDDDDDDDDDEFFDDDDD 0 79:C>T -AMELIA:165:C03TUABXX:5:1101:8676:25262 + 1 567505 GTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGA @@@FFFFFHHHHHIJJJJCHHIIJIJJJJJJGIIIJJJGHHIJJJIJJJGIIJIIIIIHIGJJHHGHFFFFFDCDCECEDCDDDDDDEEFDDDDDDDEDDC 0 73:C>T -AMELIA:165:C03TUABXX:5:1101:13584:35456 + 1 567507 GGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGC @@@DDDDFDFHHGIIJHIJJIGDHHIIJJIJIIJ@GEHGIJGIBFHGIIIGGIIJIIIIIEGGCHEEDDDEDCCECCEEDDDCCACDCCDDCDCDECADCD 0 71:C>T -AMELIA:165:C03TUABXX:5:1101:17514:29245 + 1 567508 GCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCT @<@DDD;DDHDFDHIEEEHHCGIIGIIEEBHEGHGGFEFHD@:BFDFHIIEHIIIEIDDGC@H6?CHCCCECE>;>CCCCCCC@DCDCCC>ACCCCC@A?? 0 70:C>T -AMELIA:165:C03TUABXX:5:1101:5792:4468 + 1 567514 CTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJIJJJJJJIJJJJJHHHHHHFFFFFFEEEEEFEEDDDDDEDDDDDDDDDEFE 0 64:C>T -AMELIA:165:C03TUABXX:5:1101:5398:9297 + 1 567514 CTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTT CCCFFFFFHHHHHJJJJJJJJJJJJJJJIJJJJJIJJJJIIJIIJJJJJJHHIJJIJHIJHIIHHHHHHFFFFFFEEEEEFECDDDDDEDDDDDDDDDEFE 0 64:C>T -AMELIA:165:C03TUABXX:5:1101:16134:25588 + 1 567514 CTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJIJJJJJJIJJJJJHHHHHHFFFFFFEEEEFFEEDDDDDEDDDDDDDDDEFE 0 64:C>T -AMELIA:165:C03TUABXX:5:1101:7948:43533 + 1 567514 CTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTT @@@BDDDBFAFFHI<EGH>EB@E@FGIGIDHIICGHEFHIG@DHGEHIEG=CFBFHGIID@@EECCEEABCEEDDA;6>A@;;ACCACD>;>?ABC(>>CC 0 64:C>T -AMELIA:165:C03TUABXX:5:1101:19199:4870 - 1 567517 GCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCC CDDDDDCDDEDDDDD@EDEDEDCFDCC?GGHHIHEIGGGGGIGHEJIJHIGDJJIIJJJJIGGEJJJIHIIJJJIGJIIHHHGHDIIJHHHGGFFFFFCC@ 0 39:C>T -AMELIA:165:C03TUABXX:5:1101:4847:15431 - 1 567517 GCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCC DDDDDDDDDEDDDDEEEEEFEECFFFFFHHHHJJIJJHIHJIIJIIJJJJJIJJJJJJJJIIHEJJJIHFJIJJJJJJJJJJJJIJJJHHHHHFFFFFCCC 0 39:C>T -AMELIA:165:C03TUABXX:5:1101:5279:25701 - 1 567517 GCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCC DDDCDDDDDEDDDDECEEEFEDEFFDDDHECHGHDIJHHGHHFGBJIIIIIIJIJIGJJJIGGEGIIIHHIIJJIJJJJIIJIIGJIJHHFHGFFFFFC@B 0 39:C>T -AMELIA:165:C03TUABXX:5:1101:12460:29259 - 1 567517 GCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCC DDDDDDDDDEDDDDEAEEEFEFFFFFFFHHHHIGHJIHHHIHGGCIIIEHHHJJJJIIJIIGGFJJIIHCIIIIJJJJJIHJJJIJJJHHHHHFFFFFCCC 0 39:C>T -AMELIA:165:C03TUABXX:5:1101:20467:57740 - 1 567517 GCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCC DDDDDDDDDEDDDDEEEEEFEFFFFEFFHHHHJIJIIGJHIIHJJJJJJJIGJJJIJJJJJJIGJJJIHHJJJJIIJJJJJJJJJJJJHHHHHFFFFFCCC 0 39:C>T -AMELIA:165:C03TUABXX:5:1101:6738:18984 - 1 567524 ATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAG CDEDDDDECCDDEEDDEEEEEDFDFEFHFHHIHIHGHHJIIHJJJJJJJJJJJJIHFJJIIGHIIJIJJIJJJJJIJJJJJJJIJJJJHHHHHFFFFFCCC 0 46:C>T -AMELIA:165:C03TUABXX:5:1101:10791:54191 + 1 567527 AGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAG @@@FFFFFHGHHGJIJIJJJIJJIJJIJIJGIIGIIGGIIJJJJJIIIJJJJJJJJJJIIJBHHHHHHGEF>CCDCEBCCDDDDDDEFDDDDDDDDDDDCD 0 51:C>T -AMELIA:165:C03TUABXX:5:1101:20273:5367 + 1 567533 CTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCA CCCFFFFFHHHHHJJJIJJJJJJJJJJJJIJHIJJIJJJJJHJJJJJJJJJJJJJJJJJJJJJJJHHHHHHFFFFFEEEEEEEDDDDDDDDDDDDDDDDDC 0 45:C>T -AMELIA:165:C03TUABXX:5:1101:13339:5740 + 1 567533 CTCATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCA CCCFFFFFHHHHHJJJJIJJJJJJJJIJIJJGIJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJHHHHHHFFFFFEEEEFFEDDDDDDDDDDDDDDDDDD 0 45:C>T -AMELIA:165:C03TUABXX:5:1101:9850:17758 + 1 567535 CATCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATT CCCFFFFFHHHHHJJHJJJJJJJJJJJJJHIJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHHHFFFFEEEFFFEEDDDDDDDDDDDDDDDDEE 0 43:C>T -AMELIA:165:C03TUABXX:5:1101:11172:7770 + 1 567542 AGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGAT @CCFFFFFHGHHHJJJJJJJJJIJJJJJJJJJGIJJJJJJJJJJJJJJJJJJJJJJIJJJIIJIJGHHHFFFFFFFEDEEEEEDDDDDDDDDEEEEDDDDD 0 36:C>T -AMELIA:165:C03TUABXX:5:1101:15356:12663 - 1 567548 CGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCC DEEEDDABBDDCC@DEFCFFDHHHHIJJIJIHFJJJJHFJJJJJJJIJJJJJJJJJJIHDJJJIJJJJJJJJJJJJJJJJJJJIIJJJHFHHHFFFFFCCC 0 70:C>T -AMELIA:165:C03TUABXX:5:1101:17107:28661 + 1 567549 GTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCT CCCFFFFFHHHHGIJHGIJJJIGIIGIJGIJIIJJIJIJJJJJIJJJJJJJJJGGJJJJJJIJJIJIJHHHHHFFFFFFDDDEDDEFEDDDDDDDEDDDDC 0 29:C>T -AMELIA:165:C03TUABXX:5:1101:7981:34809 - 1 567552 CTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATT CCAB?BA>C?DDC?EEDDFFFFFHHCHHEIIHHHFEEIIIIIIIJIIJIJIIIIGEHCJIIJHIFJIIGGIIGGIJIHIGEHEGCAHGGHHHHFFFFF@@@ 0 74:C>T -AMELIA:165:C03TUABXX:5:1101:17166:10414 + 1 567555 CACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTC CCCFFFFDHHHFGHHGIHIIHIICFGIIIIJGIJJJGBGGEGHEIGIGGECHIIICFHIIJJJJJIIJGEHHFHFFFFEEFEEEDEDCCEDEDDDBDDEED 0 23:C>T -AMELIA:165:C03TUABXX:5:1101:5414:44106 + 1 567556 ACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCA CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJHHHFFFFFFEEEEEEEEDEDDDDDDEFDE 0 22:C>T -AMELIA:165:C03TUABXX:5:1101:2118:46795 + 1 567556 ACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCA CCCFFFFFHHHHHJJIJJIJJJJJJJJJJJIJJJJJIJJIJJJJIJJIIIJIJJJJJJJJJIJJJJIJJJIJHHHEFFFFFEEEEEDEEDEDDDDDDEFDD 0 22:C>T -AMELIA:165:C03TUABXX:5:1101:1362:48053 - 1 567557 CGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAG DDCDCDEFBDDDDEEEEFFFFFHHHEHHIIJJJIIIJJIJJJJJJJJJJIHJJJJJJJIIJJJJJJJJJJJJJJJJJJIJIJJJJJJIHHHHGFFFFFCCC 0 79:C>T -AMELIA:165:C03TUABXX:5:1101:10057:60367 - 1 567557 CGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAG DDDDDDEEDDDDDEEEEFFFFFHHHHHHIHJIJJJJJJJJJJJJJJJJIGFJJJJJJJJJJJJJJJJJJJJJJJJIIJJJJJJJJJJJHHHHHFFFFFCCC 0 79:C>T -AMELIA:165:C03TUABXX:5:1101:5935:51253 + 1 567560 CACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCT CCCFFFFFHHHHHIIIJIJJJJJJJJJJIJJJHIJJJJJIJJIIIJIJIJHIJJJIJJJJJJIIJJIJIIJJJJJHEHHHHHFFFFFFEEDEEEFDEDDDD 0 18:C>T -AMELIA:165:C03TUABXX:5:1101:13536:14608 + 1 567563 GTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACA CC@FFFFFHHHHGJJJJJJJJJGJIJIJJJJGGIIJJIGIIIIGIJIIIJJJIIJJJIJJIIIIJJJJJGEHIIGHIHHHHHHHFFEFEEEEDDEDDDDDD 0 15:C>T -AMELIA:165:C03TUABXX:5:1101:20535:23810 - 1 567563 GTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACA CEDDDBDDDDDDEEEEEFFFFFHEHHHHIGJJJJIJIJIJIGIIIJJJJJJJJJJIIJJJJJGJIIJJJJJJIHHIJJIJJJJJJJJJHFHBHFFFFF@@@ 0 85:C>T -AMELIA:165:C03TUABXX:5:1101:13044:31031 - 1 567569 CGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAG BCACCCDCCCCACC@CA;A@DFDDFHFFECFEGHGIHEIGGIJIIIHFGGCHAJJIJIJJIHDEEIIHIJJHEJIHHFHHGIHEIEJJHHHFFDDFFF@@@ 0 91:C>T -AMELIA:165:C03TUABXX:5:1101:10293:41830 - 1 567569 CGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAG DDDDDDDDDDDCDDEEEDEEFFFFFHHHHHHHJJJJJIIJIIJJIJJJJJJJJJJJJIJIJJJIIGIHGJJJIJJJJJJJJJJJJJJJHHHHDDAFFF@BB 0 91:C>T -AMELIA:165:C03TUABXX:5:1101:20217:58615 - 1 567569 CGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAG BCCCCCC?>:C>CC@;;.;>;C?D??==3?>@HCDE>D@IGF@FB8@=<FGDG>>GFB9@@FB9<IHFCGECIHGCEFEA4HC@@BDDFB?C@;A<DD@?? 0 91:C>T -AMELIA:165:C03TUABXX:5:1101:7144:15016 - 1 567571 TTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGAC CDDDDDDDDCDDDDEDEEEEFFFFFHHHHHHHJJJIHJJIIJJJJJJJIJJJJJJJJIIJJJJJJIJJJJJJJJJJJJJJIJJJJJJJHHHHFFFFFFCCC 0 93:C>T -AMELIA:165:C03TUABXX:5:1101:3137:32408 + 1 567576 GCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAAC @@@FFFEFHFHHDIJEFHGIJIEGEGEHGJGGIGEFHGI<EFGIGGHGIJJFGIGHJJGIIIJJICFHHGIGHCHHHJIHHAHFHFFDFEDDEDCDDDDBD 0 2:C>T -AMELIA:165:C03TUABXX:5:1101:17555:44822 - 1 567576 GCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAAC DDDCCDDDDDCDDDDEEEEEEFFFFFFHHHHHIJJJIJJJJJJJJJIJJJJJJJJJJJJJIIJJJIGIGJJJJJJJJIFJJJIJJHFEHHHGHFFFFFCCC 0 98:C>T -AMELIA:165:C03TUABXX:5:1101:15689:49106 - 1 567576 GCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAAC DDDC>DDCDDCDCDDCEDCEDEFFDDFHHFEEGDAFIEGFGHGDBEIGIJJJJJJIGGGHEDHGEIGHBJJJIIHHIHIJIGGEDCBCHHHDHFDFFF@@@ 0 98:C>T -AMELIA:165:C03TUABXX:5:1101:7864:61590 - 1 567576 GCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAAC CC>5:A:;=ADCACCC@ECD@FECDB;EA?73DGIGIGIGFDHBIIIIIIIIIHHDDB@@ABGGHIGHFIHGF?9E@BCGHEF>AIIHHHHHHEDDDD?@@ 0 98:C>T -AMELIA:165:C03TUABXX:5:1101:12896:55270 + 1 567577 CTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJIIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJIHJJHHHHHFFFFFDDDEDDDDDDDD 0 1:C>T -AMELIA:165:C03TUABXX:5:1101:20009:2768 - 1 567588 TATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAAT DDDDDDDEEDDDDDDDDDDDDDDDEEEEEFFFFFFFHHHHJJJJJJJJJJJJJJJJIJJJIJJJJJJJJJJJIHHFJJJJJJHJJIHGHHHFHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:6499:19074 - 1 567591 GTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCA DDDDEEDDDDDDDDDDCDDDDDDDEEEEEEFFFFFHHHGHJIJJIIIJJJJJIHJJJIGIIIJJJJJJJJJGGJJIIJIJJJIHHJIGHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:19894:39329 + 1 567591 GTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCA @@@DDDDBCCCDB9<CBFHHII@C?D<EFGHEHED@EECHIICECHEHD@?9DHIIGDAHIIAFECHFCCCHIG;=;CEHDE@BB?ACCC>@?BBCB@>>@ 0 -AMELIA:165:C03TUABXX:5:1101:9948:49359 - 1 567593 CCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATT DDEECDDDCDDDDDDDDDDDDDDEDEEDCEFFFFHHHHGHJIIIJJJJJIHGJJJJJJJIIGJJJJJIIIGJJJJJJIJJIHFIIIHJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:20537:30398 - 1 567597 TCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCAC DCDDDDDDCCCDDDDDDDDDDEDEDEEDEFFFFFHHHFEGHIIHGJIGIJJIIGEDHEIIGJIIGIHGGJJJIIJJIHHJGGFJIJGIHHGHHFFFFF@@C 0 1:G>A -AMELIA:165:C03TUABXX:5:1101:6452:8673 + 1 567598 CAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACT @@@FDFE>BDDFHIGGIIIIGIHGHIIIIIIIE?CCGHCHGGB<FGHAFDGGGGIHIIIIDGG:BFHGICA@GHHGEHFDFDCB?B@BCBCCCACDCDCCC 0 98:G>A -AMELIA:165:C03TUABXX:5:1101:13064:25517 + 1 567606 GCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJIJJJIJJJJJJJJIGJJJIJJCHIIGIHIJIIIIIJJJIGIIJHHGHFFDDEDDDDDDEEEEDDDDDDEDFD 0 90:G>A -AMELIA:165:C03TUABXX:5:1101:6273:19643 + 1 567607 CTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTC @@@DDBDDFHHHHIGIIGG>DHHGIIEIIGGIHIEIGFAHDHIHIIIGGGEHII>FGICFEHHGCGGGADG@ACEBD<ACCCCCCC@>CDAC>A>>ACDED 0 89:G>A -AMELIA:165:C03TUABXX:5:1101:8393:36926 + 1 567607 CTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTC CCCFFFFFHGHHGJJJJJJIJJJIJJIJJJGIJJIJJJJJJIJIJJJJJJIJJIHIIIIIIJJEIHJIIIEGHHHFFFDEDDDDDDDEEEDCDDDDEDEDD 0 89:G>A -AMELIA:165:C03TUABXX:5:1101:1602:50301 + 1 567607 CTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTC @@CDFFFFGHHFHIIJGGGEDHGIIIEIHHHEGEDFGHHGEGGCGGCHBFHGDGAGAHEBFCG@FAHIIGGCAHE=B<<CBCCA>CC;ADDDA5>:@CDDC 0 89:G>A -AMELIA:165:C03TUABXX:5:1101:13656:8555 + 1 567608 TGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCA @C@DFFFFHHGGHJJJGIJJIIJJJIJJJJJJJIIJJIJJIIGIJJJIJIJJHIJJJJJGIGIJIIJJIJIIEFHBFCEDEDDDDEEEDDDDDDDEDDEFE 0 88:G>A -AMELIA:165:C03TUABXX:5:1101:5536:64484 - 1 567611 ATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCG DDDDDDDEDDDDDDDDDDDEEEFEECFFFFFFHGHJJJIJIJJJJJJIGHDHFIJJJIIIJIIHFIHHFJIJJJJJJJJJJJJIGGJJHHHHHFFFFF@CC 0 15:G>A -AMELIA:165:C03TUABXX:5:1101:12037:15781 + 1 567618 ATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAA CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHFFFFFEFEEEEEDDDDEDEEDEEEDDDDBDDB 0 78:G>A -AMELIA:165:C03TUABXX:5:1101:19670:60133 - 1 567622 TAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTA CDDDDDDDDDCEEDDADDDDDDDDDCDDDEDEEEEFFFFFHEHIJJJJJJGJIHJIIHIIJJJJJJGJJJJIGIGIJJJJJJJIJJJIHHHHHFFFFFCCC 0 26:G>A -AMELIA:165:C03TUABXX:5:1101:7784:22212 + 1 567624 GGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAAC CCCFFFFFHGHHHJJJJJJJJJIJIJIJIJIJJJJJJIIIIJIIGIJIIIJJJIJJJGIJGIIIGGHHHHFDFFFFEEDEEEEEDEEDDDDDDBDDDDDDD 0 72:G>A -AMELIA:165:C03TUABXX:5:1101:20903:23350 + 1 567628 GCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTC CCCFFFFFHHHHHJJJJJJJJJIJJJJJJJIJJJJIJIJJJJJJJJJJJJJJIJJJIIIGIJJJIJHHHHHHHFFDEDFFEEEEDDDBDDDEDDDDDDEDC 0 68:G>A -AMELIA:165:C03TUABXX:5:1101:21329:42047 - 1 567628 GCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTC DDDCACDC@EDDDDB@BDDDCCDDDDDDEECDECB?FHECGHGJIHE@JJIHIJJIIGJIIJIGGGGHDIGEBJJIJJJIJIJJJIJJHHHGHFFFFFC@B 0 32:G>A -AMELIA:165:C03TUABXX:5:1101:10698:51777 - 1 567628 GCTTCATTCACTGATTTCCCCTATCCTCAGGCTACACCCTCGACCAAACCTAAGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTC #############################################################@D4@??B??1<C?9*914?):EC8FE9FDF8BBDAA4111 0 32:G>A,48:C>A,60:A>C,76:T>C -AMELIA:165:C03TUABXX:5:1101:15407:30057 + 1 567629 CTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCT @@<DFFDFGGFFDFGIIIIGGGGGGIIGGIIEIFGIGIIGIIIIIIIIIIIIIIIIIIIIIGGIIIGHFHHFGEFFDECDEEECBCBBCCCCCCCCC@CCC 0 67:G>A -AMELIA:165:C03TUABXX:5:1101:15339:35299 - 1 567630 TTCATTCACTGATTTCCCCTATTCTCAGGCTACATCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTT DDDEEDDCDDDDDB@DDDDDDDDDDDEEEEEEFFFFFHHHEHIHGHJJIHJJJJJJJJGIJJIIIGFJJJIJJJJJJIHHJJJJJIJJHHHHHFFFFDCCC 0 34:G>A,66:C>T -AMELIA:165:C03TUABXX:5:1101:7301:21056 - 1 567631 TCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTC DDEEDDEDDDDDDDDDDCDDDDDDDDDDDEDCBFFFFFHHHHHHCJIIJJJJJJJJIHJJJJIGGIIJIGJJJJJJJJIJIJJJJJIIFHHHDFFFFFB@B 0 35:G>A -AMELIA:165:C03TUABXX:5:1101:6438:27016 - 1 567631 TCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTC DDDDACACCDDBBBBBDDDDCCCCDCDCCECA?FFFFFHHHHHGDIGGJJJJIJIHHDJJIHIFIIHDF9EICJJIJIGIIJJIIIHHDHHHFFFFFFB@B 0 35:G>A -AMELIA:165:C03TUABXX:5:1101:11534:39454 - 1 567631 TCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTC CCAC@:::ACC?-6@?AA>:>>???@A;>>9CB;;C?DA=EC=@=(5''GC<D>AD99GGFB?GB0?HHD<D:19*@?:BGECF?BEFF<BFCB?B+B?=? 0 35:G>A -AMELIA:165:C03TUABXX:5:1101:20195:16138 + 1 567632 CATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCC @<??=BDA4?8??CEGHICHEIIG<BAAHHGDIGEGH39D@FB?D69DHFBG>@9.=FCAG:D;C>CEEHA)=?;3??CDABB=@5255@>:>A@CC@A:: 0 64:G>A -AMELIA:165:C03TUABXX:5:1101:20930:21432 + 1 567633 ATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCC C@@FFFFFFHHHHGHGIIIJ@JFGHHIIGGGIIIGJIJJJJJFJIIGIGIIIIIIGCGGCHIIIJJGHHHHCEFF@DDCACDB@?BDDDCDDDDDDDDC:A 0 63:G>A -AMELIA:165:C03TUABXX:5:1101:16883:60762 - 1 567634 TTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCG EDDEDDDDDDDDDDDDDDDDDDDCCCC;?>DEEEEFDFFHEAHHGHJJIJJJIIHJJIHIGIGJJJGJJIJJJJJIJJJJJJJJJIJIHFHGHFFFDFCCB 0 0:A>G,38:G>A -AMELIA:165:C03TUABXX:5:1101:7507:13963 + 1 567641 ATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHHFD@DDDFDDDDDDEDDDDDDDDDDDDDDD 0 55:G>A -AMELIA:165:C03TUABXX:5:1101:7265:47753 - 1 567644 TCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTC >DDDDDDDDDDDDCDDDDBADDDDCCDDDCCADCDDHGHGHGIIHJIJJJGIHIHJIJJJIEIJIFJJIGIGJJIJIGHFHGHGIGIIHHFHHDDBDD@@? 0 48:G>A -AMELIA:165:C03TUABXX:5:1101:6838:64262 - 1 567645 CCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCT DDDDDDDDDDDDDDDDDDDDDDDDDDDDDC>DDFDFHHJJJIIIJJIJIJIIJJJIJJIJJJJIHJJJJJJJJJJJJIHIJJJJIIJIHHHHHDDFFF@CB 0 49:G>A -AMELIA:165:C03TUABXX:5:1101:14216:40663 - 1 567647 CCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCG DDDDDDDDDDDDDDDDDDDDDDDDDBCA;DDCAFFHHHHJIIJJJJJIHFJJJIJJJJJJIIFJJJJJJJJJJJJIHJJJIIIHJIHFHHFFDFFFDFBB@ 0 51:G>A -AMELIA:165:C03TUABXX:5:1101:15453:35487 + 1 567655 CAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJGFHGEHJGGEHGHIJJJIIEGHIIIIEHEDFEFCCEECEEDDDDDDDDDDDDABDDDDDDDDDDDDDD? 0 41:G>A -AMELIA:165:C03TUABXX:5:1101:3755:9250 - 1 567657 GGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGG DDDDB?9DDDDDDDDDCCADBB?DDBDDDCCCDECEECC>EEFDEFEFFCHHJJIJIIJJJJIJIIHIHCFJIGIIGHIIHHHIGJGJFHHGHFFFFFCCC 0 61:G>A -AMELIA:165:C03TUABXX:5:1101:3543:5931 + 1 567659 CTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAA @C@FFFFDHBHBDEEEHIJIIIJGIIIJIGCGFGIEGIIIIJIJJHIGHIIJHBHGAEHEDDFFFFEDDEEEEDD@ABDDDBDDDDDDDDDDDDDCDBD@9 0 37:G>A -AMELIA:165:C03TUABXX:5:1101:9118:7595 - 1 567666 CTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCG CDDCDDDDC@DB@DDDDDDDDDDDDDDEDDADEEDEEEEDDDDDDFHHJJJJJJJIHHIGIJJIHJIHFJIHGBJIIHIHJJJJJIJJHHHHHFFFFFCCC 0 70:G>A -AMELIA:165:C03TUABXX:5:1101:16020:31899 + 1 567666 CTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCG @;@AD?:DHD=?FA?:CGHICCGEFEHG>F???4:?B?4D?F>??B006-5@E@EI7=C=7777..;>).56=;;;5>5;>87<@=?>@C80<5?>@:9<9 0 30:G>A -AMELIA:165:C03TUABXX:5:1101:13315:45926 - 1 567668 AGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGAC DCADB@A:B>55@CBCCCCCCCCCCACCCC>DAECADDCB@9BDDDBDFFHGHHHHGHAHA@;GHIFGDB?*DCBBIGGJIGHEJIGGHDHHGFFDFF@@@ 0 72:G>A -AMELIA:165:C03TUABXX:5:1101:13781:59309 + 1 567672 CAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTA CCCFFFFFHHHHHJJJJJJJJJJJJJJJIJJJJJJJJIIJJJIHIJJJJJJJGIIJJJJJHHHHHFFFDEEEEEDDDDDDDDDDDDBDDDDDDDDBDDBDD 0 24:G>A -AMELIA:165:C03TUABXX:5:1101:13009:62753 + 1 567677 CTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGG CCCFFFFFGHGHHJJJIJIIJJJJIIJJJJIJIIIJJJIIIIJJJJJJJIJJJJJIJJIIJHHHFFFFFFDDDDDCCDDDDDDDDDDDDDDDDDDDDDDDD 0 19:G>A -AMELIA:165:C03TUABXX:5:1101:11016:6414 - 1 567679 ACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGAC BDDDDDDDDDDDDDEEEDDEEEEEEDDDDDDDDDDDDDEDDDDCADDDBDEBDFEDHHHC?HIIGHFJJIJJJJIJJJJJJJIJJJJJHHHHHDFFFFCC@ 0 83:G>A -AMELIA:165:C03TUABXX:5:1101:19280:40287 - 1 567679 ACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGAC 5DDDDDDDDCDDCDEDDDDEDDEDCADDDDBDDDDEDDEDDDDCCDDCACC=DFFDHHEEEHIIEHFJIIJJJIHJJJJJJJIJJJJIHHHHHDFFFFCCB 0 83:G>A -AMELIA:165:C03TUABXX:5:1101:10675:25938 + 1 567682 CCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTAC CCCFFFFFHHHHHJJJJJJIJJJJJJJJJJJJJHIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHFFFFFEDDDDDDDDDDDDDDDDDDDDDDDDDDD 0 14:G>A,100:T>C -AMELIA:165:C03TUABXX:5:1101:10986:34150 + 1 567682 CCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTAC @?@DDDFDHHHHHIIIG>HHHABBHIGEHHGAG@FHFFHCGHIIGIEGIIAFHIGGHIIDEEGEH=AHFFFFFCCCBBBCCCCBB?>5<BCBCC<?BB9CC 0 14:G>A,100:T>C -AMELIA:165:C03TUABXX:5:1101:4746:62553 + 1 567682 CCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTAC CCCFFFFFHHHHHGGGIIIJJJJJJJIGIIJJJGHGGDGIJIHGIJJIJIJJIIJJGGIIIJJIIHHHFFFFFEDCDDDDDDDDDDDB>BDDACBDDBDDD 0 14:G>A,100:T>C -AMELIA:165:C03TUABXX:5:1101:21278:4747 - 1 567683 CAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACC @CC@>:@>ACCA>+CDDADDCC@C<@<@;@@;C@@@C@>:(A??B?CACBA;?;.;.A=88>F@DFF;?:6>GIHGEA@GBEEGCHEGHFADDDDBDD@@? 0 1:T>C,87:G>A -AMELIA:165:C03TUABXX:5:1101:5276:36395 - 1 567683 CAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACC A@AAB@BBBADA>:@:@:@A>>:45<<B@BBABA@A5@55(@;;5?>?>@@DBDD@?EE:8;<FFF>=FAF@D@FEEBDBBG@C<IGBBFFFDDBBDB1?< 0 1:T>C,87:G>A -AMELIA:165:C03TUABXX:5:1101:6844:19078 - 1 567688 TCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGAT CDDDCDDDCDEEDEEEEDDDDDDDDDEDDEDACCC@DDCAB?<DDDCDFCC@DC>AEDHHHIJJGJJJJJJJJFJJJJJIIJIIGJIJHHHHHDFFFFCCC 0 6:T>C,92:G>A -AMELIA:165:C03TUABXX:5:1101:10100:23071 - 1 567688 TCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGAT CDDDDEEECDEEEEEEEDDDDDDDDDECDEDDDDC@DDDADCADDBCDD@C>DDCAFDHHHJJJJIJJJJJJJIJJJJJIJJIHGJIJHHHHHDFFFFCCC 0 6:T>C,92:G>A -AMELIA:165:C03TUABXX:5:1101:11010:8161 - 1 567689 CCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATG CDDDDDDCDEEEEEEEDDDDDDDDDEDDDCCCAC@CDCADC@DDBCDDCA;DDBACBFHHHJIHGJJJJJJJIJJJJJHJJIHGJIJJHHHFDFFFFFCCB 0 7:T>C,93:G>A -AMELIA:165:C03TUABXX:5:1101:10698:46822 + 1 567690 CATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGC @@@DFDFDAFBHFEG@FHHCGEIGDG88?D@F@E?@GGGG?BGGAFGDBFBFA@FHC@GEB<CEEE=BBA=3>>,5;?88)2<8:@B005>>?C<?##### 0 6:G>A,92:T>C -AMELIA:165:C03TUABXX:5:1101:2869:6289 + 1 567701 CATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGTTGCATACACCACAT C@@DDDFFHGHFHIFEIGIHGE@FGHIGCDHGGEHIHIIIEIEEEBBEEGBEHGIIIGGEFFCEDC?BBBBCBDDB@9>B3?C8?0&+2)+44:3>A<<?C 0 81:T>C,86:A>T -AMELIA:165:C03TUABXX:5:1101:16129:58233 + 1 567703 TATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGA CCCFFFFFHHHHHIJJJJJJJJJJJJJJJJJJJJIIJJJJJJJJJJJJJJJJJJJHHHHFFFFDDDD@BDDDDDDBDDDDDDDDDDDDCDDDDDDDDDDDD 0 79:T>C -AMELIA:165:C03TUABXX:5:1101:2319:20533 - 1 567706 TCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAAC C@99B@DDDCDDDCC@@::<><<32>??<?>44(>:3'=6DDCC@<B?>@CCEE=@IHHFABHGGIGFFFAHFC8?GIIIIHGBGGGCFDHHHFFDDD@=@ 0 0:T>C,24:T>C -AMELIA:165:C03TUABXX:5:1101:10533:36385 - 1 567706 TCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAAC DDDDDDDDEDDEDDDDA@DCDBBC?DDDCDC@A:DCB?DBDDDDDDDEFFFHHHGJJJJJJIIIIHGJJJJJIIGEJJJJJJJJJJHHHFHHFFFFFF@B@ 0 0:T>C,24:T>C -AMELIA:165:C03TUABXX:5:1101:14563:55897 - 1 567707 CATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACA DDDDDDDEDDEDDDEC@DDDDDCBDBBADDAA:DDDBDBDDDDDDDDFFFFHHHHJJJJIHJIHFGJJJJJJHHEJJJJJJJJJJIHIFHHHHFFFFFBCC 0 1:T>C,25:T>C -AMELIA:165:C03TUABXX:5:1101:15908:10437 - 1 567709 TCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATC <DDDBDCDEDDDDDDDCC?BCDDDBDDDC@:DDBBBBBDDDDDDDDFFFFHDHJGGHDFJJJIGIJJIJJHG?JIIJJJJFJJJIHHIHFHFFFFFDFC@@ 0 3:T>C,27:T>C -AMELIA:165:C03TUABXX:5:1101:8029:24962 - 1 567710 CGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCC @DDDEDCDDCCDA>DDDDDDA>@<?<>3@:CC@BD@DCACCB@DCCFBFEHEIIGEIIGGG?8GGJJIHGHEJJIFIHIIIGIAC:GHFGGHHFFFFF@@@ 0 4:T>C,28:T>C -AMELIA:165:C03TUABXX:5:1101:16753:48192 - 1 567710 CGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCC DDDDDDDDDDDECCDDCBDDADBDCBDAC>DDDBDBDDDDDDDDDFFFFFGHJJJIIHIJJHHJJJJIIIHEJJJJJJJJJJIHHFIIHHHHHFFFFFCCC 0 4:T>C,28:T>C -AMELIA:165:C03TUABXX:5:1101:17172:9508 - 1 567713 CGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTAT DEDDEDDDECCDDDDDDDDDDDDDA@>DDDBDBDDDDDDDDDEFEFFFFHJJIIHJJIHGJIJJIIHHDJJJJJJJJJJJHHFJHHJJHHHHHFFFFFCCC 0 7:T>C,31:T>C -AMELIA:165:C03TUABXX:5:1101:3891:58164 + 1 567713 CGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTAT @@?DDDDBFHHBFHIEEHHHIIGEHGGIIIIIGIIGIIGCHBBFEHIIEECCFHGIF=?CDEAAB@B@?CCAB@@<@@ADACC:?A<A@@AA@CCCCCCC: 0 69:T>C,93:T>C -AMELIA:165:C03TUABXX:5:1101:3852:60798 + 1 567713 CGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTAT CCBFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJHFFFFEEDDDDDDDDDDDDDDDDEDDDDDDDDDDDDCDDDDDDDD 0 69:T>C,93:T>C -AMELIA:165:C03TUABXX:5:1101:17789:21387 + 1 567714 GTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATC @=@DDDFDHFDFGJEGHGGIIIJJGGGBAGHGIGAFHHEIGE@DGGGECGHGBHBHEFF?BD?CCB>ACCBDDB9>BDEDCC@CBDDDDC>CCCDACDCDD 0 68:T>C,92:T>C -AMELIA:165:C03TUABXX:5:1101:8017:43974 - 1 567714 GTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATC ECDECDDDC@DDDDDDBDDDDDDCACDDDBDBDDDDDDDDDEFEEFFFHJJJIHIJIGIJJJJIHHGDJJJJJJJJJJHHHGJHHJJJHHFHHFFFFFCCC 0 8:T>C,32:T>C -AMELIA:165:C03TUABXX:5:1101:19698:9860 - 1 567718 ATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCT CCDDC@CAA<BCADDBADDDCCDDDBD@DDCDDB@>DCFEEEDFHHGHFCIGHC?HDIGGBEF?JJJJIIJJIHDFFAIHJJJHJIIGHHDHHFFFFF@B@ 0 12:T>C,36:T>C -AMELIA:165:C03TUABXX:5:1101:4701:5877 + 1 567724 CTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJHHHHFFFDEEEDDDDDDDDEDDDDDDDDDDDDDDDDDDDDDDDEDDDEEDDC 0 58:T>C,82:T>C -AMELIA:165:C03TUABXX:5:1101:7860:20851 + 1 567724 CTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGC CCCFFFFFHHHHHIJIIIJJJJIIIJJIJIJIJJJIJIIJJIJGJJJJJHHHHFFFDEEEDDDBDDDDEDDDDDDDBCDDDDDDDDDDDDDDEDDDDEDCD 0 58:T>C,82:T>C -AMELIA:165:C03TUABXX:5:1101:20745:24324 + 1 567728 CTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCAT @?@FDDFFFHDHDHIEIBCFHBEHADEBF<:?699BB6D::4;@E;CDAC6?>@CC@688?@BCDACCCCB?<>>@@3>944?:>4:::@ACD:(>C:ACC 0 54:T>C,78:T>C -AMELIA:165:C03TUABXX:5:1101:19341:63570 + 1 567728 CTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCAT @@?D?DDDDHHFDEGIDFCBGIIIIIIIIIGIIIIIIGGBHHHGIIGIFHHFEFEEACB?@=@>>CCCCC8AACCCCCCCCCCCCCCCDCCC@CCCCCC@C 0 54:T>C,78:T>C -AMELIA:165:C03TUABXX:5:1101:19719:29197 - 1 567730 TCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTC BDDBADDCC>CC<<DBDDDDDDDBDDFDDDBDDDFHHHJJIGEHHJJJGHGDJJJJJJIIGGHGGCIHHIIIJJIHJJJJJJJJJJIIHHHHHFFFFDC@C 0 24:T>C,48:T>C -AMELIA:165:C03TUABXX:5:1101:9482:45341 - 1 567730 TCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTC DDDDDDDAC@DDDBDBDDDDDDDDDDFDDDDDDDFHHHJJIHDJIJJJIJIHJJJJJJJJJGGFGEIHIJJIJJJIIJIJJJJJJJIJHHHHHFFFFFCCC 0 24:T>C,48:T>C -AMELIA:165:C03TUABXX:5:1101:8831:57320 - 1 567730 TCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTC BDDDCDDA@:A?95BBDDDDDDDDBDFDDDDDDDFHHEJHHG@JIJJIIHF@IIJJJGGIIIHGHGIHHJJJJJJJJJJJJIJJJIJJHHHHHFFFFF@@@ 0 24:T>C,48:T>C -AMELIA:165:C03TUABXX:5:1101:2935:26890 - 1 567732 CCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCAT BDDBB@>:CCBBDDDDCDD@@<BCCCCDDDDDDFHEHIIIGIIIIGGF;?IIHBHFBEGIHHHFIIIHIGIHHEHGHGHCHCIH@HEIHHFHFBDFDD@@@ 0 26:T>C,50:T>C -AMELIA:165:C03TUABXX:5:1101:19748:33676 - 1 567733 CACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATT ?CDDAA:CC>BDDDDDDDDDDDDDCCDDBDDDFHHHHIECJJJJIGIHDJJJJJIJJIEHFHEJHGIJIJJJIJJJJJGJIJJJJJJJHHHHHFEDFFC@B 0 27:T>C,51:T>C -AMELIA:165:C03TUABXX:5:1101:19868:3468 - 1 567734 ACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTT 9DD@>4CDDBB>BDDDDBBDDDDCCA=BDDCFHCHHEE;GHIIHHFF;JJIJJIJIIGFCD?IHGIIIJJHHIIIGGIJJJJJJJJIEHHHGGFFFFFCCC 0 28:T>C,52:T>C -AMELIA:165:C03TUABXX:5:1101:10983:9401 - 1 567734 ACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTT ABD@C>CDBBDDDDDDDDDDDDEDCCBBDDDFHHHHHHDJIJJJIIHFJJJJJJJJIIIGGFJHGJJHJJJHIIJJJJJJIJJJIJJIHHHHHFFFFFCCC 0 28:T>C,52:T>C -AMELIA:165:C03TUABXX:5:1101:2552:33425 - 1 567734 ACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTT 9A>4>4CC>9DDDDDDDD@DDCAC>??@DDDFEEHHHHDJIIHHHIIHGJJIJHHIIFDDD:EHFJIEIIJIJIGIHGHHGGHHEHJIFFF?HFFFFDCC@ 0 28:T>C,52:T>C -AMELIA:165:C03TUABXX:5:1101:6704:39251 + 1 567735 CAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTC CCCFFFFFHHHHHJJJJIIJJJJIJJIJIJJJJJJJJIIIJJJJIJHHHHFFDDDDDEDDDDDDDDDDDDDCCCDCCDDDDEDDDEEDDDDDDDEEEEEEC 0 47:T>C,71:T>C -AMELIA:165:C03TUABXX:5:1101:17001:56434 - 1 567735 CAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTC BC@CADDDDDDDDDDDDDDDDFDC?8BDDDFFHHHHHEJIJJJJIHFJJJJJJJJJJIGGDIHHJJIJJJIJJJJJJJIJJIJJJJJJHHHHHFFFFFCCC 0 29:T>C,53:T>C -AMELIA:165:C03TUABXX:5:1101:16393:11380 - 1 567741 TTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAAC CBBDBDDCDDDDDDDEDDCDDDDDBADFFEB?HHJIIHIHAJJJJJJJJIIHFF?IHGJJIJJIHHFGJJJJIJJJJJJJJJIJIHHFDHHHHFFFFFCCC 0 35:T>C,59:T>C -AMELIA:165:C03TUABXX:5:1101:10186:22061 + 1 567744 CTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGC CCCFDFFFFHGHHJJJGGIJIJIIIIIIJIIGIIIJJJJJJJJHHFFFFFEEEEDDDDDDDDCCCDDCDDDDEDDDEDDDDDDDDEDEEEEEEDDEDDDDD 0 38:T>C,62:T>C -AMELIA:165:C03TUABXX:5:1101:4099:62541 - 1 567756 GGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAAT DDCDDDBBB?<=DDCA?C?FFFD?>=HIGIIIFJJIHGFBHDFIIFDGGFIGIGGJIGGHGE@JJIIIGIHHHHIJJJFIIIEIHIJJHHGHHFFFFFC@@ 0 50:T>C,74:T>C -AMELIA:165:C03TUABXX:5:1101:7804:16054 + 1 567757 GAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATA @CCDFFFFHDDFHHIJIGIJJJJGHIIJIHIIGGEGGFHIJIIIIHHGHEHDFFFFFEECEDEACC@CCDC@DEEECDDEDDEDDCDDDDCCCDDDDDDDC 0 25:T>C,49:T>C -AMELIA:165:C03TUABXX:5:1101:18741:6904 + 1 567763 CCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTC CCCFFFFFHHHHHJJJJJIJJJJJIJJJJIJJIIIGIIJJJJJIJJJJJJJHHHHHHHFFFFFFEEEECEFEEEDDEDDDDDDDDDDEEEEFEEDDEEEDE 0 19:T>C,43:T>C -AMELIA:165:C03TUABXX:5:1101:2710:4535 + 1 567764 CCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCA CCCFFFDFHHHHHJIJJJJJJJJIIJJIJJIGIIJGIJJIJJGGJJJJEHEGIJHHHHHHFFFFFFEEEEEEEEDDDDDDDCDCCDFEFEEDEEDEEEDE@ 0 18:T>C,42:T>C -AMELIA:165:C03TUABXX:5:1101:11389:8845 + 1 567783 CCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGC CCCFFFFFHGHHGJGHIJIGGIJJJJJIJJJHHJIIIDGIJJJJJJJJJIIJIGIJGIIJJJJJEHIJJJJIJJIHIJIFHHHHHFFFFFEDDEEEDDCDD 0 23:T>C,83:A>G -AMELIA:165:C03TUABXX:5:1101:19348:5016 + 1 567785 CGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTT @@@DFFFFHHGHHIIIEGHIIIJIIIJIGAGFHIGCFHBDFIGIJJGIJIGIIJJJJGIJIIDGGHEHIEHIJJFHIGIJGGHHGHFFFFFFFDEEEDDDB 0 21:T>C,81:A>G -AMELIA:165:C03TUABXX:5:1101:18964:45121 - 1 567797 CACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAG CDDEFDEDDDDDDDEEDDDDEEEEECFFFDFFGHHHHJJJJIJJJJJJJJIJIIJJIJJGJJJJJJJJIJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC 0 31:A>G,91:T>C -AMELIA:165:C03TUABXX:5:1101:17286:14466 - 1 567804 AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAAT DDDDDDDEEDDDDDDDDDDDEEEEEEFFFFFFHHGHHHJJJJJIJJIJJJJIIIIJJJJJJJJJJIJJJJJJJJJIJJIJJJJJJJJJHHHHHFFFFFCCC 0 38:A>G,98:T>C -AMELIA:165:C03TUABXX:5:1101:2302:26509 - 1 567804 AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAAT CCDDDDDEEDDDDDDDDDDDEEECC>FFFFFFFHHHHHJJJJJJJJJJJJJJIJIJJIJJJJJJIJJJIIJJHJJJJJJJJJJIJJJJHHHHHFFFFFCCC 0 38:A>G,98:T>C -AMELIA:165:C03TUABXX:5:1101:14914:27015 - 1 567809 CCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAG DDEFDDDDDDDDDCCCCDDCCEEDEFFFFFFEHHHHHJJJJJJJJJIJJJJJJJJJJJHEJIJJIJJIJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC 0 43:A>G -AMELIA:165:C03TUABXX:5:1101:17154:32662 - 1 567809 CCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAG CDEFDDDDDDDDDCDDDDDEEEEEEEFFDFDEHEGHGIIJIJJHGJIJIIJJJIJIGGIEGJIIIJIGJHJJJJIIIJJJJIIIJJJJHFGHHFFFFFCCC 0 43:A>G -AMELIA:165:C03TUABXX:5:1101:13477:42130 - 1 567809 CCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAG CCDDCDCDDDDDDDDDCDDEEEECCCDFFFEHHHHHHGGIIIIJIIHEIJJJJIIIIHIGJIJJJJJJJJIJGIJIJJJIJJIGIJJIHHHGHFDFFFC@B 0 43:A>G -AMELIA:165:C03TUABXX:5:1101:17026:8151 - 1 567811 TATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAA DB:::,5593>5>;>55;36(;3@6).)B?B>C=;;:@AE@==77;F==.=.=.)=@ED>B@?03D6:0F?)=G9GE@D>C3,C:DC<<FCF<BBD=1?;; 0 45:A>G -AMELIA:165:C03TUABXX:5:1101:7273:6801 - 1 567816 TCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGAACC ADDCCCCDDCDCCDDDDDEECCCEBCBCDDFHHHFGHEIHGGGHGIJJIHGIJHGIGEIIJJIJJJHIGGGJGIIJIIIIIGIJIIJIHGHHFFFFFDB@@ 0 50:A>G -AMELIA:165:C03TUABXX:5:1101:14854:16404 - 1 567846 GTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATG CEEEEEEEFECCCCCCCCCCCFCCC@CCECCCEDAHDHDAEBHBFAHIHGGGGGIIGHFBEIHDC3GBCIGIGIIIGHF?IIGHHFFCDHDFHDFFDB@@@ 0 80:A>G -AMELIA:165:C03TUABXX:5:1101:3416:27002 - 1 567850 TATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCC DEEEEEDDDDDDDDDDDDDDEDDDDDCDDDFFHHHIJJJJIJJJJJJJIJJJJJIJIGJJJIGGCJJIIJJJIJHGJHHJJJJIJJJJHHHHHFFFFFCCC 0 84:A>G -AMELIA:165:C03TUABXX:5:1101:17364:20474 + 1 567863 CATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACA CCCFFFFFHHHHHJJIJJJJJJJJJJJJIJIJJIJHIJJJJIJIHDEHIJJJJJIJJHHGFHHHFFFFEECEEDDCDDEFDDDCDDDDDDDDDDDDDDDDB 0 3:A>G -AMELIA:165:C03TUABXX:5:1101:15209:9364 - 1 567869 TTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCG DEDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDEEEDEDDDDDDBA<DDDCDDDDDDCDDDDDDDEEDDEDDDDDDDDDDJJIHFAJIGFAHFFEDFFF@CB 0 -AMELIA:165:C03TUABXX:5:1101:14144:25234 - 1 567869 TTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCG CCCACCCBBBBBBBBBBBBB>B@CCCCAACCDEEDCDCACCCCA<8@?<>CCAAA@@CCCCC@A@>>CDCAACCCC916IGHFA2HGBBHBHHDDDDBC@@ 0 -AMELIA:165:C03TUABXX:5:1101:19906:34008 - 1 567871 GAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCGAA DDDDDDBB@DDBDBDDDDDB>DC@CCCADDDCADDDDCACC882DBADDDDCCA8ACADDDDCC>DDCACCDDCDDDGBHBF?F@AC<FHHHFFEDDD@@@ 0 -AMELIA:165:C03TUABXX:5:1101:14922:24532 - 1 567872 AGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCGAAG DDDDDDDDDDDDDBDDDDDDDDDDDDDDEEEEDEDDDDDDDB?DDDDDDDDDCCDDDDDDDEEDDEDDDDDDDDDDJJJHGCJJHFEIFFHFHFFDFFCCB 0 -AMELIA:165:C03TUABXX:5:1101:20743:56858 - 1 567872 AGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCGAAG CCCCBBBB<@>??8>BBBBBCCCCCCCCEDDDCECCCCC@?<8BBBCCCCCCCCCCCCCCCDDCCDCCACCCCBBBIHGHFAIHGGCEFFDAA>DDDD@@@ 0 -AMELIA:165:C03TUABXX:5:1101:7729:59534 - 1 567872 AGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCGAAG ADDC?B@<BDDDDDDDDDDDDDDDDCDCDDCDDDDDDCDDBA8DDDDDDDCC@8DDCDC>@3CBDDDCDDDDDBBDIIJIHEJIJHGHFCHFFDDFFFB@@ 0 -AMELIA:165:C03TUABXX:5:1101:4473:62226 - 1 567878 CTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCG DDDDDDDDDDDDDDDDDDDDDDEEEEDEDDDDDDDB?DDDCDDDCDC>DDDDDDDEEEDEDDDDDDDDDDJIJHFAJHHHGIGFHGJIHHHHHFFDDFCBB 0 -AMELIA:165:C03TUABXX:5:1101:11622:12783 - 1 567890 GCGAAAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACATAAAAT BDDDDDDDDDDEEECDDCDDDDB<0DDBADDDCC@:DDDDDDDEEEDEDCDCDDDDDDIGGAB:JHGDCHFCGEIGCEJJJIHFCJJJGHHHFFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:1422:33140 - 1 567891 CGAAAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACATAAAATC BCCCCCC@CC@EECECCCC@CA8(><<:@C>?CCCC@ACCCCCDCC@CCCCCAC98BGIHG?*IHFD?GE:C?FFA;IIGHGF<IIHFBCCCHDDDDD@<@ 0 -AMELIA:165:C03TUABXX:5:1101:2831:33074 - 1 567894 AAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAG DDDDDDEEEEDEDDDDDDBB?DDDDDDDDDDCDDDDDDDEEDDEDDDDDDDDDDJJIHD?JIHFCHGCIGJJIHJJJJIHFJJJJJJIHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:21133:63887 - 1 567894 AAAGTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAG ACA@::CCCCCDCCCCCCB?2<@AA>:C?CA:AAACCAC@@3@>@ACCCCC@BBIEF<@BIGCIIHAHFEBIGGFIGIHG@HFBHHEFDGHFHFFFFFCC@ 0 -AMELIA:165:C03TUABXX:5:1101:14330:15655 + 1 568144 TGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCC @@@DFADDDHHHDIJCFGHIJJIJGDHGIJIHIJJJIGIIIIJJIJJFJFIJJIIJJJHIHHHGHHHFFFFFDDDDDDDDDDDDDDEEDDDDDDCDDDDCC 0 56:T>C -AMELIA:165:C03TUABXX:5:1101:10371:24068 + 1 568149 CGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTC @??DDDDDFHFFHIJJJJIIJIGHGHIHIIJIIIHGHGFGIGHHIJIJJJIJJJGIGEEGIIIJGGGHG<BBBDF?CACEEDCEDDDDDDEDDDDDDDCAA 0 51:T>C -AMELIA:165:C03TUABXX:5:1101:5106:55589 + 1 568149 CGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTC @@@FADBDHFFFFGHIIJJJGIGHIIIIJJJIIJIJJIJJIJ@DFIGGGHHHJIJIJJIJJJIGIGGJHHFDFFFEECCCCEEECC@CDDEDDDDCCDD@@ 0 51:T>C -AMELIA:165:C03TUABXX:5:1101:10510:58071 + 1 568149 CGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTC CCCFFFFFHGHHHJJJJJJJJJJJJJJJJJJJJJJJIJIJJIGIJJJJJJJJJJJJJJJIJJIJJJJJHHFFFFFFEEEEFEEEDDDDDDDDDDDDDDDDC 0 51:T>C -AMELIA:165:C03TUABXX:5:1101:9272:42176 + 1 568150 GCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCC @@CFFFFFHBHHHJJJJIJJJJJJJJIJJJIJJJJIJJIJJCHJJJJJIJJJJJJJJJJIJJJJJGIJHHHHFFFFFFFEEEEEDEDDDEDDDDDDDDDDC 0 50:T>C -AMELIA:165:C03TUABXX:5:1101:16828:18921 + 1 568153 AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGT BCBFFFDDHHHHHJIJJJJJJJJJJJJJIJJJJJJJJJJJJJIJJJJJJJJJJJJIJJJJJJJJJJHHHHHHFFFEFFFEEEEEEEEDDDDDDDDDDDDDD 0 47:T>C -AMELIA:165:C03TUABXX:5:1101:6227:33420 + 1 568153 AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGT @@?ADB=BFFFDFDHGGHABFGHDHHGHFEGHHECHICFAFIIB@GHEHEECGIIGHFFDHEHGB8AEAHEF@?BDCDEACCC<AADCCCCCCCCCACCCC 0 47:T>C -AMELIA:165:C03TUABXX:5:1101:3902:21992 + 1 568154 GTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTA @@@FFDDFFHHHHIIIIGIJJIJIJIIGGGHIIIIIIIIIIJHGGIIIIJIJIJJGHGIHEHHIHIGHGHHHFFFCEFFCECECCDDDDDDDDDCCDDDDD 0 46:T>C -AMELIA:165:C03TUABXX:5:1101:14681:7537 + 1 568155 TAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTAT CCCFDDFFHHHHHJJJJJJJJJJJJJIJIJJJIJJIGGJJJJJJJJJJJJJJJJJJIJJJIJJJJJHHHHHHFFFFFFFEEEEEEEDDDDDDDDDDDDDDE 0 45:T>C,100:C>T -AMELIA:165:C03TUABXX:5:1101:4488:49575 + 1 568158 GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCC CCCFFFFDFHHGGGJIIIIHHGGGIIJIJGGGDHGGIIAFHHGGDHGIGIIIHIGGIIJGGIEGHIHEHHHHGFFFFFFEDEDEECCDDD>CCCDDCDD>@ 0 42:T>C,97:C>T -AMELIA:165:C03TUABXX:5:1101:16334:14441 + 1 568160 CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCT CCCFFFFFHHHHHJJJJJJJJJJJJIJIIJJIIJJJJJJJJJJJJJJJJJJJJJJJIJJJIJJJIIDHHHHHHFFFFFFFEEEEEEDDDDDDDCDEEDDDD 0 40:T>C,95:C>T -AMELIA:165:C03TUABXX:5:1101:4678:20514 + 1 568160 CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCT @@@DDDFFFFHHHJIJJIIJIJGGGIEGGIGEIGGGFBGII@GHIIBIGEGGGGHIEGAHGIIJIICEFFHEHFFD@DDFECCEDECCCACCD@CDDDCCC 0 40:T>C,95:C>T -AMELIA:165:C03TUABXX:5:1101:4357:22241 + 1 568160 CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCT @@CFDFFDH>HHHIJIJIIGIJIIIJGGIJJIJIJJIJJJJHGIJJIIJIJJJJJJ<@BFDHIIJJGGHHGGHFFDEFFFCEEEEDDCDDDDD(;ADCDDC 0 40:T>C,95:C>T -AMELIA:165:C03TUABXX:5:1101:12637:53892 + 1 568160 CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCT CC@FFFFFHHHHGIJIJJJIIIIIIIJIJJIIGIIIEHIGIGIJJJIJGGIJJGGH1AGHIIIGIIHGHHHGGEFFFFFFEEDCEECCDDDDDDDECCDC> 0 40:T>C,95:C>T -AMELIA:165:C03TUABXX:5:1101:6058:10341 + 1 568163 CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTT :?@DA:DD?:8CF@AA?CG?AHHI<?:CG@B:9D<?9??F@??*?4B?9997?;;4@FD@C===77@>;;7A3;?B).?B;6@C>6;AB;A>>55@BBB>: 0 37:T>C,92:C>T -AMELIA:165:C03TUABXX:5:1101:11320:14943 + 1 568163 CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTT CCCFFFFFFHHHHJJJJIJJGGIGIEGEGHIJJIJJIJIJIIHJJIJIJIIJJIGIJIGIIIIJIIIHHHHHHHDDFEFFEEECECEDDDCDDDDDDDDD> 0 37:T>C,92:C>T -AMELIA:165:C03TUABXX:5:1101:13874:21410 + 1 568163 CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTT CCCFFFFFHHHHHJJJJJJJJJJIJJJJJJJJJIJJJJJJJJJJJIJJJJJJJJJJJJJJJJJIJIIHHHHHHHFFFFFFEEEEEEEDDDCDEEDDDDDDC 0 37:T>C,92:C>T -AMELIA:165:C03TUABXX:5:1101:14662:31020 + 1 568163 CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTT CCCFFFFFHGHHHJJJJJJJJJIJIJJJDFIJJFGIIJGIJHIJIIIJJJJCHIIIJIJJIJJGIJIGHHFHHHFFFEFFECCCECEDDDCDDEDDCCDDC 0 37:T>C,92:C>T -AMELIA:165:C03TUABXX:5:1101:14507:39864 + 1 568163 CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTT CCCFFFFFHHHHHIJJJJJJJJJIJIJJHIJJJJJJJJJJJJJJJJIJJIJIJJJJJJJJJJJJJJJHGHHHHHFFFFFFEEEEDEEDDDDDEEDDDDDDD 0 37:T>C,92:C>T -AMELIA:165:C03TUABXX:5:1101:12461:43409 + 1 568163 CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTT CCCFFFFFHHHHHJJJJJJJJJJJJJJJHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIIJJJHHHHHHHFFFFFFEDEEEEEDDDDDEEDDDDDDD 0 37:T>C,92:C>T -AMELIA:165:C03TUABXX:5:1101:6888:50163 + 1 568163 CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTT CCCFFFFFHHHHHJJJJJGJJIJJIJJJGIJJJJJIGIJJJJJJJJJJIJJJJJJJJJJJJJJJJJJHHHHHFHFFFFFFEEEEEEEDDDACEEDDDDDDD 0 37:T>C,92:C>T -AMELIA:165:C03TUABXX:5:1101:4360:53358 + 1 568163 CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATGACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTT CCCFFFFFHHHHHJJJIIJIGIIJJJJIIIJJIJJEIJIJJJIIJJJJ*BHGJJJIJJJJJJIJJHHHHHHHFFFEDFEEEEEEECDDDDCDDEDDDDDDD 0 37:T>C,48:C>G,92:C>T -AMELIA:165:C03TUABXX:5:1101:15256:54171 + 1 568163 CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTT CCCFFFFFHHHHHJJJJIJJJIIGGIJIGIIJJGIJJJIJIJJFIGIJJJJJJJJGIIHGGIJIJIJHHHHHCHFFFFFFEEECECCDDD@CDECDDDDDD 0 37:T>C,92:C>T -AMELIA:165:C03TUABXX:5:1101:10326:61091 + 1 568163 CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTT C?@FFFFFHGHHGJJGIGJIJIJJIJCFGIIJJJJJJJIJJJJJIJJJGIJDGIIJIJJJJIJJJJJGGGHHGHFCDFFFEEEEECCDDDDDEEDDCCCCC 0 37:T>C,92:C>T -AMELIA:165:C03TUABXX:5:1101:4506:62288 + 1 568163 CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTT CCCFFFFFHHHHHJJJJJIJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHHHFFFFFFEEEEEEEDDDDDEEDDDDDDD 0 37:T>C,92:C>T -AMELIA:165:C03TUABXX:5:1101:16643:28494 - 1 568164 AAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTC DDDDDDDCDDDDDDDDEEDDEEEEEDEFFFFFFFECHHGJIJJIIGEIHJJJJIIJJIJIFJIHIGEHIIJJJIJJJJIJJJIHIIIGHHHHHFFFFFCCC 0 9:C>T,64:T>C -AMELIA:165:C03TUABXX:5:1101:11633:21804 + 1 568165 AGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCC CCCFFFFFHHHHHJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJIJJJJJJJHHHHHHHFFFFFFEEEEEECDEEDDDDDDDDD 0 35:T>C,90:C>T -AMELIA:165:C03TUABXX:5:1101:2196:14228 + 1 568168 CGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAA CCCFFFFFHGHGHJIJIIIIIJIDIIJIHEHGIIIJJHIGIJJJIIJJJIJJJIIIEGHIHIJIIIJDIGJJIJHHGGHGFFFEFCEEEEECDCCDDDDDC 0 32:T>C,87:C>T -AMELIA:165:C03TUABXX:5:1101:7307:36708 + 1 568169 GCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAAC CCCFFFFFHHGHGJJJIIIJJIIIJJJIJIJJJJJIIIJGIIIJJGJJJJJIJJJIJJGIJJJJJJJJJJJJJJJHHHHHHHFFFFFFEEEEEEDDDDDDD 0 31:T>C,86:C>T -AMELIA:165:C03TUABXX:5:1101:6270:2692 + 1 568173 CTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTC CCCFFFFFHHHHHJJJJIIIJJJJJJJJJJJJJIJJJJIJJJJJJJJJJJJJJJIJJJJJJJJJIJJJJJJJJJJHHHHHHHFFFFFFEEEEEEDDDDDDD 0 27:T>C,82:C>T -AMELIA:165:C03TUABXX:5:1101:19343:19870 - 1 568175 TCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCAC DDDDDEEDEEDDDDCDDDDDEDECAEEFFFFFFFHHHGJJJJJJJJJJJJJJJJJIJJIJJIJJJJJIJJIJJJJJJJJJJJJJJJJJGHHHHFDDDDB?B 0 20:C>T,75:T>C -AMELIA:165:C03TUABXX:5:1101:14284:25696 + 1 568187 GAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAAC @@@DDDFFHFHGGGJIJJIJFIFJIIJJGIIJJJJIGIGHIJIIJGGIGIIIIJJJJIIIGGHIJGFHEA>F@GHGHHEHGEFCEFFECECDBBDDDDDDC 0 13:T>C,68:C>T -AMELIA:165:C03TUABXX:5:1101:13688:5293 + 1 568188 AAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJIIJJJJJJJJJJJJJJJJJJIJIJIJJGGIJJJJJJJIJHHHHHHFFFFFEEDDDDDDDDDDD 0 12:T>C,67:C>T -AMELIA:165:C03TUABXX:5:1101:9841:15858 + 1 568190 GAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAA CC@FFFFFFHHHHJJJJJJGIJJJJJJJIJIJJJJJJJJJJJJJJIJJJJIIJGJJJJJJJJJJJJIJJJJJIJJIGHHEEHHFFFFFCDBEDDDCCDDCA 0 10:T>C,65:C>T -AMELIA:165:C03TUABXX:5:1101:16954:32477 + 1 568190 GAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAA @<@=?;BDB>FFFFEBBBHHI:<C:FBFAEEFBFE<4CDB@FAEFFBA?DFF@DFF88?FFEE<C:4=@@C@@DECC@.=7?>@DD@@@?(;=?ABAAB@: 0 10:T>C,65:C>T -AMELIA:165:C03TUABXX:5:1101:3906:14232 - 1 568191 AGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT DDDDDCC>:BCDCCCCDDCDBCDFFHGFHHIHGECIGIIGIIIHEEIIIHGEIIIIIIHGEIHEIHGEGIIIIIIHHHECHFGFHFFHBFD?FDDDFF@@? 0 36:C>T,91:T>C -AMELIA:165:C03TUABXX:5:1101:13950:37302 + 1 568193 CTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATAC CCCFFFFFHHGHGJJJJJIJJJJJJJJJJJJJIIJJJJJIJJGIJJJJJJJJJJIGIIJIIIJJJIIJJJJIIGGEHFHHHHFFFFFDEDEDDDDDCDDDD 0 7:T>C,62:C>T -AMELIA:165:C03TUABXX:5:1101:8001:64132 + 1 568193 CTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATAC CCCFFFFFHHHHHJJJJJJIJJJJJJJIJJIGIGIJJJJJJJGIIJJIJJIHJJIIIJJJHGIJIJJJJJJJJJJGIHHGHHFFFFFDEDEDDDDDDDDDD 0 7:T>C,62:C>T -AMELIA:165:C03TUABXX:5:1101:19300:30388 + 1 568195 TATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTA CC@FFFFFGHHHHJIJJJJGHJJIIIJIIJJJJJJJIJIIEIJJJIJJJIJIHBFHIIGIJJIJJJJIJJIIGGIIJHHEFFFFFFDDEDEDDDDDDDDDD 0 5:T>C,60:C>T -AMELIA:165:C03TUABXX:5:1101:8964:39421 + 1 568196 ATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAA CCCFFFFFHHHGHJJJJJJJJJJJIIJIIJJIJJJJGGJJJIJJJJ@GGIGHDGGIIGGIJJIJJJJJJJJEIJJIJHHHHHFFFFDEDEEDDCDDCDDCC 0 4:T>C,59:C>T -AMELIA:165:C03TUABXX:5:1101:9415:43836 - 1 568197 TCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAAC CCABCCC@DDEEDDDBDDEFDFHHEEHHEEJJIGJJJJIFIIIIIIJJIJJJJJJJIGJJIJJJJJIJIJJIHFIIHFIIHJJJIHJIHHHHHFFDFF@?B 0 42:C>T,97:T>C -AMELIA:165:C03TUABXX:5:1101:6915:17304 + 1 568198 CACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACA CCCFFFFFHHHHHJJJJJJJJJJJJJJJIIJJJJJJJDHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHIHHHHFFFFEEDEEEDDDDDDDDDD 0 2:T>C,57:C>T -AMELIA:165:C03TUABXX:5:1101:5732:37223 + 1 568201 CTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCT CCCFFFFFHHHHHJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJHIJJJIJJJJJJJJJJIJJJIJJJJJJJJHHFEEFFFFEEEEDEDDDDDDDDDC 0 54:C>T -AMELIA:165:C03TUABXX:5:1101:16888:10058 + 1 568214 GCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGA @@@FFDDDDHFFBFBFEGGGHCHIDGHIIEHGCG@FGHIBFGIIIGHIGICHHG@GIIHH@FAFGH@=@EHIEIIBACCHHGFF);@CDDCEEDDDDDDDD 0 41:C>T -AMELIA:165:C03TUABXX:5:1101:18912:37166 + 1 568229 TTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTG CCCFFFFFHHHHHJJJJJJGIJJJIIJJJJJJJJJJJJJJJGJIJIJJJJIJJJJJHIJJJGIJJJIJJIJIJIIJIHHHFFDEEEEDDDDDDDDDDDBDD 0 26:C>T -AMELIA:165:C03TUABXX:5:1101:1469:4067 + 1 568232 CTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAAC C@CFFFFFHHHHHJJJIJJIJIIJJJJJJJJJJJJJJJGJJJJJJJJJIIJJJJJJJIJJIJJJIJJGIIJJJJJHHHFFFEEEEEEDDDDDDDBBDDDDD 0 23:C>T -AMELIA:165:C03TUABXX:5:1101:17330:12822 + 1 568232 CTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAAC CCCFFFFFHHGHGJIJEHIJJHIIJIJJJJJIJJIJJJGIIJGIIJJJJJJJIJJJIJIIIJGEIJJJJJJJJJJHHHFFFEEEEEEDDDDDDDBBDDDDD 0 23:C>T -AMELIA:165:C03TUABXX:5:1101:8313:19544 - 1 568238 TGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTACCCT DDDDDDDDDDDDDDDDDDDDFFFFFHHHHHEGCIGIHJJIIJJHFJJIHJIJIIGJIIJIHFHGJIHGJJJIJJJJJJJJJJJIHJJIHHHHHFFDBFCCB 0 3:T>C,83:C>T -AMELIA:165:C03TUABXX:5:1101:14043:2168 - 1 568242 TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCC DDDDDDDDDDDDDDDDDDEEEEFE@ACCCHEHEIHHJJIHFIHGAJIJIHDJJJIJJIHGJIHHJJJJJJJJJJJJJJJIHIHGBJJJHHHHHFFFFFCCC 0 87:C>T -AMELIA:165:C03TUABXX:5:1101:7565:6141 + 1 568249 CCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCA CCCFDFFFHHHHHIJJJIJJJJJJJJJJJJJJJJJIIIJJJIJJJJIJJJJJJJJIJJJJJJJJJJJJHHHFHHFFFCDEDDDDDDDDDEDDDDDDDDDDC 0 6:C>T -AMELIA:165:C03TUABXX:5:1101:3391:32824 + 1 568249 CCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCA CCCFDFFFHHHHHJJJJJIJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJIJHIJJJJJJJIJHHHGHHFFFCDEDDDDDDDDDDCDDDDDDDDDD 0 6:C>T -AMELIA:165:C03TUABXX:5:1101:5259:35715 - 1 568255 TGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAG DDDDDDDDDDDDDDDCDDBCDDDBCCDCCCCCDCFC>>CBCDDC@C@DDB@DEEFFFFFFHHGHHHHBJJJIHIGJJIJJJIJJJJJJHHHHHFFFFFCCB 0 100:C>T -AMELIA:165:C03TUABXX:5:1101:1450:8904 + 1 568257 CCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTC @<@DDADBCD<<;ED>FAFFAFHH33@BF>EHGGG<<?BDF9B30B?D>CHBG:@CGF@FFEE;=@A6=59@?@CC>CC>@:A3553;;B<>@CC###### 0 -AMELIA:165:C03TUABXX:5:1101:6851:33287 + 1 568267 AACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCC =<@D1BDD>?FB;;C=FGHIGAADHF99FEGEBBGHHCH9)?@@:?98BF9CCFEHB84-4=D=;777=3;@?7?A>ABB;ACCA@CCA:(:>@44+9?## 0 93:T>C -AMELIA:165:C03TUABXX:5:1101:3810:19847 + 1 568278 CAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCACCCTTAATCCAGGCCCTCATCGCCTCCCCACCCCTA @@<BD8DDF<<A:E?<EEH@FBEH9CD<EDFEFGGGHGGDFDGGGFB?F68;=@G>;CH>@E@H##################################### 0 64:C>A,65:G>C,68:A>T,70:C>A,75:T>A,76:A>G,78:T>C,82:T>C,89:C>T,90:T>C,95:T>C -AMELIA:165:C03TUABXX:5:1101:15659:10442 + 1 568279 AAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTAC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJIJJJJJJGHHFFFEEEEEEEDDDDDDDDDDDDDDDDDDDDDDDD 0 81:T>C -AMELIA:165:C03TUABXX:5:1101:15758:21042 + 1 568279 AAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTAC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJHHFFFEEEEEEEDDDDDDDDDDDDDDDDDDDDDDDD 0 81:T>C -AMELIA:165:C03TUABXX:5:1101:2093:3151 + 1 568283 CTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCAT @?@DD8BADDFF?<GDEF:4A@FFIF@FD?CF)9?C<D449B@08?DDFE>B8B3).7@FEEE';7@DDDDC>>(66>3@@?582'9??############ 0 77:T>C -AMELIA:165:C03TUABXX:5:1101:8680:29054 + 1 568287 CTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJHIJJHHHHHFFFFFFEEEEEEEDDDDDDDDDDDDDDDDDDDDDDD 0 73:T>C -AMELIA:165:C03TUABXX:5:1101:21126:18637 - 1 568289 AATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTA CCADCBCDCA>CCDB?5BCCCDCDBAC>CC>BB>BDC@C@>C@CCC?DBBDBDGGAEHHAHBEGEIGGIHEJIIIIGIHEGEGHDGEGFDHHHEDFFF@@@ 0 29:T>C -AMELIA:165:C03TUABXX:5:1101:19979:56142 + 1 568289 AATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTA CCCFFFFFHGHHHIJJJJJJJJJJJJJJJJJJJJJIIHHIJJJJJJJJIJJJJJJJJJJHHHHHHFFCFFFCDEECDDDDDDDDDDDDDDDDDDDDDDDDD 0 71:T>C -AMELIA:165:C03TUABXX:5:1101:15115:27724 - 1 568292 ACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACAT @DDCDDCCDCDBBDDDDCDDEEEEEEDDB@5DDDDDEDDCDDDDDDDDDFGIJJJJJIHJIIJJJJJIJIGJJJJIIJJJJIJJIHFJHHHDHFFFFDCCB 0 32:T>C -AMELIA:165:C03TUABXX:5:1101:13293:56238 - 1 568292 ACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCTATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACAT CDDCCDDCDDDDDDDDDDDDEDEEDEDDBB9DDDCDEDDCDDDDDDDDFHHG@4JJJIIJJIJJJJJIJJIJIJJJIJJJIHJJIJHJHHHHHFFFFFCCC 0 32:T>C,47:C>T -AMELIA:165:C03TUABXX:5:1101:10645:11712 + 1 568293 CTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATA @@@DDBDFHFHDDHJGIJGHGEBFHCHGGHJJJCDHIGGGCGGIHECDHEEEE;DGHIJFFHHHEDFFFFFEDCDDDDDDAC@B>>A>>?B<A@CDAACCD 0 67:T>C -AMELIA:165:C03TUABXX:5:1101:13067:26688 + 1 568293 CTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATA CCCFFFFFHGHGHIIGIIIIJFGGHIGIJJIIIFGHCIIJIJIGIJGGJJGIIJJJJGIGGHE?EEFFFFFCDDDDDBBCDDDDDCDDBDDDDDDCDDDED 0 67:T>C -AMELIA:165:C03TUABXX:5:1101:1890:41433 + 1 568293 CTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATA @@@?DDDDAFFFB@G@81CGADGHDG?<?FBHGHGGG>FG<<9B@BBEHAGDHI;C===AHEE7?)7@?BD@=9;;??BC:?BBC:95908>CCC>ACCCD 0 67:T>C -AMELIA:165:C03TUABXX:5:1101:16498:55364 + 1 568293 CTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATA CCCFFFFFHHHHGIJJJJJJGJJJGJGIIJJGIHIJDGIIIJJIJIJJJJIJJJJIJIJHHHHEHFFFFFFCCDDDDDDDDDDDDDDDDDDDDDDDDDDEE 0 67:T>C -AMELIA:165:C03TUABXX:5:1101:2645:16516 + 1 568297 CATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAG C@CFFFDFHHHDFGIJIJGGIEHIJJI@HIDHEE<GDCDDFHBFHIIHIBHIG;=CDEHAHHGEHDFED<ACD?BCDDDDDCDDDB@BCCCCCDDEEDCDC 0 63:T>C -AMELIA:165:C03TUABXX:5:1101:10791:28160 + 1 568297 CATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAG @@@FBEFDFFHG<EHGIGEFGIGIIIICHHGIGGIGCHH@;FBCHGGGIFFGHIIIGGHHHEHCHFFBD<9>ABB;>CBBCCBB?@?B3>CCAC>@CACC@ 0 63:T>C -AMELIA:165:C03TUABXX:5:1101:15055:38356 - 1 568297 CATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAG CDADCDDB>BDDCDDEEEEEEDDDB>DDCCCDDDDDDDBDDBDDDHHGHJGJJHJJIHF>IHEIGGJJJJHFIIGFFIIHHEIHIGGJHHHHHFFFFDCC@ 0 37:T>C -AMELIA:165:C03TUABXX:5:1101:12897:3090 + 1 568300 CTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACG CCCFFFFFHHHGHJJJJJJJJJJJJJIJJJJJJIJJJJJIJJIIIJJJJJIJJJJJIJJHHFHHHFFDDDDDDDDBDDDDDDDDDDDDDDDDDECDDDDDB 0 60:T>C -AMELIA:165:C03TUABXX:5:1101:18209:9219 + 1 568300 CTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACG CCCFFFFFHHHGHJJJJJJIJJJJIJHIJIJJJJJIJIIIJJJIJJJJJJJJJJJJIJJHHHHHHFFDDDDDD@CBBCCDDDDDDDDDDDDDEECCDDDD> 0 60:T>C -AMELIA:165:C03TUABXX:5:1101:5712:11910 + 1 568300 CTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACG CCCFFFFFHHHHHJJJJJJIJJJJIJIIJJHGIIJJIIIIJJIJIJJIJJIJJJJJHHHHHEEHEFFCCDDDDDDDDDCDDDBDDDDDDDDCDDCDDDDDB 0 60:T>C -AMELIA:165:C03TUABXX:5:1101:19301:30702 + 1 568300 CTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACG CCCFFFFFHHHHHJJJJIJJJJJJJJIIJJJJJJJJJJJJJJJIJJJJJJJJJIJJJJJHHHHHHFFDDDDDCDDDDDDDDDDDDDDDDDDDEEDDDCCDB 0 60:T>C -AMELIA:165:C03TUABXX:5:1101:8090:31032 + 1 568300 CTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACG @@@FFFFFHHHHHJJJJJJJJJIJJJGHIIJJJJJJJIIGIJJIJJJJJJGGHGIJHIJHGHHHEFFD>BCDCDDBDCCDDDDDDDDDDDDDDDDDDDDDB 0 60:T>C -AMELIA:165:C03TUABXX:5:1101:9216:46192 + 1 568300 CTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACG @CCFAEDFFHFDDFFDFGEF@HFBBE:CFCGGGC<BBFCFGHHHEHBHI@GGGIHEDECEHEEEE=B>CCBBCCCAA>C?:?BBBCCACCCCEE@CAC>AB 0 60:T>C -AMELIA:165:C03TUABXX:5:1101:20611:58724 + 1 568300 CTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACG @@@DDDDDFF8<<EADD4CHIIE?CG)?GC?@FB9B9BG>GF866@@5@;=78C;..7).77.=.,,99@;;;;=55>@9=B<B594+4@:A@BBBBA3<5 0 60:T>C -AMELIA:165:C03TUABXX:5:1101:10876:44667 - 1 568302 CAGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAG DDDBDDDDDDEEEEEEDDDB>DDDCCEDDCDDDBDDDDDDDFFFFHHGHHJJJIHJHFIHHIGJJHFJJIHFJIHHGJJJIGJJJJJIHHHHHFFFFFCCC 0 42:T>C -AMELIA:165:C03TUABXX:5:1101:14917:50436 + 1 568303 AGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGG CCCFFFFFHHHHHJJJIIJJJJJHIJJJJJJJJJJJJIJJIJJGIIJJJJJJJIJIJJJJJHHHFFFDDDEDDDDDDDDDDDDDDDDDDEEDDDDDDDDDD 0 57:T>C -AMELIA:165:C03TUABXX:5:1101:16889:8099 - 1 568309 TCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACG CCDEEEEEEDDDB9DDDDDEEECDDCDDDDDDDDDEEEEEEFFFFFHHHHHJJJJJJJIHJJIIIJIHGGIGJIGJJIIIHJJJJJJJHHHHGFFFFFBCC 0 49:T>C -AMELIA:165:C03TUABXX:5:1101:5320:43715 + 1 568310 CAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGA @@@FFFEAHBFHDEHECEHHGFHGCC>GHGIIIIGIIIIIIIIFGHBFGGEDGGEAHGHIHGHCCDEEFECCB@BCCCCCCCCDCCCCCB<B<>)9::>>B 0 50:T>C -AMELIA:165:C03TUABXX:5:1101:13027:59934 - 1 568311 AGGAAACAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTCCGCATCCTTTACATAACAGACGAGGTCAACGAT >>BA>4(B>50><BABBD@4(?4(0<<57B@8?A;,5>5(;55?;(CBC@99:-/@;9CBC3BFD?1)E?122CA4IHCFC<A:GEGGCBBA?D:AAD@=8 0 33:A>C,51:T>C,94:T>C -AMELIA:165:C03TUABXX:5:1101:8737:36281 + 1 568312 GGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATC @@@DD;?DDHHHHAFHIHIGEHGI<E>EGICAFG@DHHGGBG<BDGEHGGCGCFGGEAAHC;AEBC?@861;>CC>>;@CDCCC@CC<9<>B5?C>:082< 0 48:T>C -AMELIA:165:C03TUABXX:5:1101:3136:2038 - 1 568314 AAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCN EEDCCDBB>DBDCCADDDDDC<B<3BDBDCDCEDDDCEECEDCEB?EEAHGIHF?GHFD?HGFDEHCHCEHHHGJJJJJJIIGGEGGJFHDHHDFDDA=1# 0 0:C>N,54:T>C -AMELIA:165:C03TUABXX:5:1101:21406:10702 - 1 568314 AAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCN EEEDCC@85>BDDDEEEDDDDDD@BBDBBCC>EDDDDDEDECBDFEFFHGHEBGBIGHGBDIJJIJIHFCGHGGECIGIJJJIJJJJJHDFGHFFFDB=1# 0 0:C>N,54:T>C -AMELIA:165:C03TUABXX:5:1101:2387:3108 + 1 568317 TAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCC C@CFFFFFHHHHHIJJJJJJJJJJJIJJJJJJJJJJJJIIIIIIIIJIJJJJJJJIJJJHHHHFFFDDDEDDDDDDDDDDDDDDDDB>CCCDBBDD?CCDD 0 43:T>C -AMELIA:165:C03TUABXX:5:1101:18730:33146 + 1 568319 GAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCT @@@FDFDDFHHDHJIJGGGHEIGHGGGGI@GGGECHEDGFG@BHCBFGHACHGIG;6=3?EE>BB<>CEEDDDD;@CDD<B=@B@5?C<CB@BABCDDBB? 0 41:T>C,99:T>C -AMELIA:165:C03TUABXX:5:1101:19892:21474 + 1 568325 GTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCAT C@CFFFFFHHHHHJJJJJJIJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJIEHHHFFFFFFFEEEEEDDDDBDDDDDDDDDDDDDDDDDDDDC 0 35:T>C,93:T>C -AMELIA:165:C03TUABXX:5:1101:2894:3543 + 1 568327 CTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCA CCCFFFFFHHHHHJJJJJJJJJJJJJJJJIJJJJJIJJJJJJJJJJJJJJJJJJJGJHHHHHHFFFFFFEEEEDDDD?CDDDDDDDDDDDDDDDDDDDDDD 0 33:T>C,91:T>C -AMELIA:165:C03TUABXX:5:1101:8199:6476 + 1 568327 CTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCA CCCFFFFFHHHHHJJJJJJJJJJJJJJJJIJJJJJJJIJJJJIJJJJJJJJJJJJIJHHHHHHFFFFFFEEEEDDDDBCDDDDDDDDDDDDDDDDDDDCDC 0 33:T>C,91:T>C -AMELIA:165:C03TUABXX:5:1101:7341:24683 + 1 568327 CTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCA @@@B?D?AFFHDFIGBGBGE@A?CHD9?C9C@DF9FG>GB@@GGI@<FHHFHIEBC@AEEHEHEBB;?@C@@;8383,95@CB?ABBC@C@BC@CC>4:CC 0 33:T>C,91:T>C -AMELIA:165:C03TUABXX:5:1101:12434:18494 - 1 568329 GAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAA DDCDCC@DDBB@BDDCDDCDCDCCDDDCDCCDDBDDDFD>HFEEHIHF@@GGFDIIIEIEBEICHFEHGDGIJIHGEIIIGHGGJHGIGFHFDFFEAF@@? 0 11:T>C,69:T>C -AMELIA:165:C03TUABXX:5:1101:18828:29161 - 1 568330 AACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAAT CADC@<DDBDDDDDDCCCC@CDCCC<CDCCDB?DDDFD>HHFEAIGG=GIHEEFGHEGCEGIEIIGFDGJIHFHF>JIIGGGJIIGJIHDDFDFFFFF@@@ 0 12:T>C,70:T>C -AMELIA:165:C03TUABXX:5:1101:13578:52417 - 1 568330 AACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAAT DCDDDDDDDDDDDDDDEDDDDDDDDBCDDDDDBDDDFFDHHHHIJJIHGJJJJGJJJIGHJJGJIFJIJIJJJJJIJIJJJIJJJJJJHHHHHFFFFFCC@ 0 12:T>C,70:T>C -AMELIA:165:C03TUABXX:5:1101:21327:5756 - 1 568331 CCTATCCTGCCCTCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACTCAGCCTTTACATAACAGACGAGGTAAACGATCCCTCCCTTACCATCAAATC #####################################################DB<D?/*B4?9)3GGB@FD::)?1?A3@HDE<B@GDD<DC4D4B=111 0 13:T>C,26:C>A,48:T>G,51:G>T,71:T>C,88:G>T,100:A>C -AMELIA:165:C03TUABXX:5:1101:18409:12410 - 1 568335 TCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATT DDDBDDDDDDDEDDDDDDDDDDDDCBDDDDDDDEFFFHCHHIHFJIIGGJJJJIIJJIJJIIHHJIGJJIJJHJIIGJJIHJJJIGEJHHFHHDFEFFCC@ 0 17:T>C,75:T>C -AMELIA:165:C03TUABXX:5:1101:9209:15403 - 1 568335 TCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATT 7><9B>B@?CCDCCCACCCC?:C@:8?6BBBBA9EBBA=FFGHGGDB<2F=4<DFGGCD?;HDGEGBCCGGDFD=G6GHGCCHFFC<GFD>HHDD?DD@@? 0 17:T>C,75:T>C -AMELIA:165:C03TUABXX:5:1101:16696:20851 - 1 568335 TCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATT ?>B>5BB@?CAACCCCCCA?ACACA?BBBBBCCCDB=EEHE@5-FC==BAF=8B?GGHCFFDB3IGGEGGGIIHFB@IGEBIHEEC<IHFHHHDDADD@@@ 0 17:T>C,75:T>C -AMELIA:165:C03TUABXX:5:1101:14311:28399 - 1 568335 TCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATT BDDBDDDDDDDDDDCDDDDDDDDDDDDDBDDDDFFFFHAGHIEGJJIJIJJJIIIJJIJIJIGDJJJJJJJJIIIIHJJIGJJIHGCJHHHHHFFFFFCC@ 0 17:T>C,75:T>C -AMELIA:165:C03TUABXX:5:1101:13911:41584 + 1 568336 CCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTG CCCFFFFFHGGHHJIJJJJIHHIGHIJEAGHGHHIJGFIIGGDHIJIIIGFHIIJJGIHHHGHFFFDD>CCCCBDDDDDDDDDDDDDCDDDCCCDCDDDDD 0 24:T>C,82:T>C -AMELIA:165:C03TUABXX:5:1101:10839:49530 + 1 568337 CTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGG CCCFFFFFHHHHHJJJJJJHIJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJHGHHHFFDDACDDDDDDDDDDDDDDDDDDDDDDDDDDDDDEDC 0 23:T>C,81:T>C -AMELIA:165:C03TUABXX:5:1101:9833:14546 - 1 568338 TGCCCGCCATCATCCTAGTCCTCATCGCCCGCCCATCCCTACGCATCCTTTACATAACAGGCGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGC ######################################DDC;67A8@/..//))>).2?00DCD?9:)1)::9)<<3<3:<<32<:4EDDD==:BD:???? 0 20:T>C,40:A>G,70:T>G,78:T>C -AMELIA:165:C03TUABXX:5:1101:13179:39588 - 1 568338 TGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGC 9DDDDDDDDCC@ADDDDBCDDCDB??>DBC@CFFE@FHHGEJIIHBJIHEFBEHCHBEEECJGEGJIIIIIIHGJJJIJJJJIFIIIIHHHHHFFFFDC@@ 0 20:T>C,78:T>C -AMELIA:165:C03TUABXX:5:1101:3360:18056 - 1 568339 GCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGCC BDDDDDDEDDDDDDDDDDDDDDDDDDDDDEDDFEDFHFFGJJJIIJIJJJIJIJJIJIHGJIIJJJJJJIJJJIIJIJJJIHFJIJJJHHHHHFFFFFCCC 0 21:T>C,79:T>C -AMELIA:165:C03TUABXX:5:1101:14464:22275 - 1 568339 GCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGCC BDDDDDDEDDDDDDDDDDDDDDDBDDDDDEDDFFDFHHHHJJJIIJJIJIHJIIIHJJHHJJJJJJJJJJJIHJJIIJJJJHIJJJJJHHHHHFFFFFCCC 0 21:T>C,79:T>C -AMELIA:165:C03TUABXX:5:1101:13035:22507 - 1 568339 GCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGCC DDDDDCDDCDCCDCACA@CCCDB?B@>BC@CBD>?DHGHGHEF;>IIIGIIIIGEIJGGHIHDFDIIIGBDGEHHG?IJIICEIIIIIHHHFDDDFDF@@@ 0 21:T>C,79:T>C -AMELIA:165:C03TUABXX:5:1101:6073:36225 - 1 568339 GCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGCC BDDDDDDEDDDDDDDDDDDDDDDDDDDDDCDDFDDFHHHEJIIGIJJJJJJJJIIIJIHHJJJIJJJJJJJJIJJJJJJJIJIJJJJJHHHHHFFFFFCCC 0 21:T>C,79:T>C -AMELIA:165:C03TUABXX:5:1101:8097:58155 - 1 568339 GCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGCC DDDDDDDEDDDCDDDDDDDDDDDDDDDDBADDFFCFHHHEJIJIEIJJJIHJIIIHIGGIJIJJJJJJJJJJIJJIHJJJIHIJJIGJHHHFHFFFFFCCC 0 21:T>C,79:T>C -AMELIA:165:C03TUABXX:5:1101:2371:10266 - 1 568359 TCAGCGCCCTCCCATCCCTACGCATCCTTCACACAACAGACGAGGTCAACGACCCCGCCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTAC #########################################################C?111**F?**BC<AC?<+33BEC<C:+<2+FFC<A;A2==:11 0 18:T>C,41:T>C,44:T>G,48:T>C,67:T>C,71:T>C,97:T>G,99:T>C -AMELIA:165:C03TUABXX:5:1101:2823:38048 - 1 568421 ACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGA #A@A:>::::3CC?><8(8ACCCC>5A>3AC;@C?=56?CDB;9;=EGIHE<IGGGEIIIGGEAF@GHEEIGGIHFG<F9GG>HDIIIFBF?DD3DDD?@; 0 59:A>G,80:T>C -AMELIA:165:C03TUABXX:5:1101:6844:40956 + 1 568424 ATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACC CCCFFFFEHHHHHDIIIJGGEIJJFBHIJJJJJJIJIIJBFGHJIIJJJJGJJIED@BDCDCCDD@DC@CDCDDDDCDDDCDDDDDD?CCDECDDCCCDD< 0 17:T>C,38:A>G -AMELIA:165:C03TUABXX:5:1101:18653:49808 + 1 568424 ATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACC CCCFFFFFHHHHHJJJJJJJJJJJHIJJJJJJJIJJJGJDHGIJIJIJJJJJJIHDDDDDDDDDDEEDDDDDD4+:@DDDDDDDDDBDDCDEEEDCDDDDD 0 17:T>C,38:A>G -AMELIA:165:C03TUABXX:5:1101:12494:36849 - 1 568426 CAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAG DEDEDDDDBDDDB@@DDDDDDDEDDDDCCDDDBDEEEECFBFHECIGAJJJJJJIJJJJIIJIIHGHFJIIGJIGGIGGJJJJIJJJIGHHHHFFDDFC@@ 0 64:A>G,85:T>C -AMELIA:165:C03TUABXX:5:1101:7127:62906 + 1 568426 CAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAG CCCFFDDFHHFDDHGHHHGIIIHGHIJJJIJGGIEBC:BBFHIGGGGHC@BHHDDDDDCADDCCACDDCCC@@>C>A:>CCCCBDCDDDDEDDCCCCDA<< 0 15:T>C,36:A>G -AMELIA:165:C03TUABXX:5:1101:6722:4998 - 1 568427 AAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGG EDEDDDDDDDDDDDDDDDDDDEFDDDCADDDADDFEECBBFHHCHGHJIJJJIHJJJJJJJJIIHGDIIJIJJHFIIHJJJJJJJIJJHHHHHFDDFFCCB 0 65:A>G,86:T>C -AMELIA:165:C03TUABXX:5:1101:17384:8499 - 1 568427 AAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGG ##################CC:C@>>5(:8;=2B@>;(/=9<BAA5@;A=FCD?4GFGCGGFD4B9BFGFFDFF>CB??H?GGE@FIIIHFDAHEDBAD<<@ 0 65:A>G,86:T>C -AMELIA:165:C03TUABXX:5:1101:9428:16177 - 1 568427 AAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGG EDEDDDDBDDDDBADDDDDDDDCDDCCADDDBDDFEECDFFHHHHIHJJJJJJIJJJJIJIJJIHHFIIGGJJIHIIGJJJJJJJJJJHHHHHEEDFFCCB 0 65:A>G,86:T>C -AMELIA:165:C03TUABXX:5:1101:19563:30398 - 1 568427 AAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGG DDEDDDDDDDDDDCDDDDDDDEDDDCC@DDDBDDFEEBDDFHHHHJIJJJJJIHJJJJIJJJJIIIGJIIHJGHFIIHJJJJJJJJJJHHHHHFFDFFCCB 0 65:A>G,86:T>C -AMELIA:165:C03TUABXX:5:1101:11835:45302 - 1 568427 AAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGG DCCC>>98?B?@BCCCCCCCCEFCA>>>B@=;BC@A>3A9FHEHCE;GFADHFBGHEHCHBEGHDHGIIIHDFD<GIHECIHHBG<A?<BGHDDDBDD@@@ 0 65:A>G,86:T>C -AMELIA:165:C03TUABXX:5:1101:7518:50033 + 1 568428 AATCAATTGGCCACCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGC CCCFFFFFHHHHHJHGGHJIFHHJJIJIJIJJJIJFGGCHHJIIJJJJJGIDDDDDDCDDDEEDDDDDDDDDDEDDDDDDDDBCDDEEEEDCDDDDBADDD 0 13:T>C,34:A>G -AMELIA:165:C03TUABXX:5:1101:2216:16934 - 1 568431 CAATTGGCCACCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGAC DCC?CDDDDCCDDDDC@DDCDDCCDBDBBDCD@DB>CBDBDAAEHGC@HCJIHIIIHAGGGHDGHFDDFB?GDCGIJJHHBGGGGJHCHHFCFDDDFF@?@ 0 69:A>G,90:T>C -AMELIA:165:C03TUABXX:5:1101:16383:11591 + 1 568432 AATTGGCCACCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACC CCCFFFFFHHHHHIJJEHIJJJJJJJIJJJJFHIJJJJJJJJJJJIJFDDDDDDDDDEEDDDDDDDDDDEDDDDDCDDDDDDEEEEDDDDDDDDADDDDDD 0 9:T>C,30:A>G -AMELIA:165:C03TUABXX:5:1101:2402:11777 - 1 568438 CCACCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGAC B@ADDCDDCCCC@ADDDDBBADCCC@>8;@:>7B@BDFFEEEEHFHHBIJJIIIHFIGHCIGIFIHHJIIJIJIGHEGIJHJHGHIFIFHHFHFFFFD@@@ 0 76:A>G,97:T>C -AMELIA:165:C03TUABXX:5:1101:9989:61267 - 1 568438 CCACCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGAC BB@DDDDCCDEDDDDCADDDDBDDC>@B=DBD?BBDDFDFHHHGHHJIIIIIHHHGHFGBGJIGGFFJIJIGIIIGJJJJIJHHFJJJHHHFFFFFFF@@@ 0 76:A>G,97:T>C -AMELIA:165:C03TUABXX:5:1101:5680:4985 + 1 568441 CCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCC CCCFFFFDHHHHHJJJJJJJJJHIJJJJJJJJIJJJIIIHFFFEEEEEEEDDDDDDDDDDFDDDDDDDDDDDDDEDDDDDDDDDDDDDDDDDDDDDDDDDD 0 0:T>C,21:A>G -AMELIA:165:C03TUABXX:5:1101:5428:39099 - 1 568442 CAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCT DDDDDDEDDDDDDDDDBDDEDCBB9DDDBDDBDDDDEFEFFFFHHHHHHHIIJJIGIIHCIGHJJJJJJJJJJJJJJJJIHJJJJJJJHHHHHFFDFFCCC 0 80:A>G -AMELIA:165:C03TUABXX:5:1101:14794:60613 - 1 568444 ATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTG DDDDDCDCCC@DDB<DDEEDDDDDB@?D@=DDDCE@CEFFEDFDEHHCECJIHDIG@7FA;IJJIHCD@IGDHF@EIIJJJIIIFGCFHHHFFDDDFFC@@ 0 82:A>G -AMELIA:165:C03TUABXX:5:1101:5503:42677 - 1 568453 AACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACA DCDDDDDDEDCBB@DDEDDDBDDDDDDDDDDDDDDDCC>CAEDEBFEC?HHBJJIJIJIJJIIJJJJIIGJJIJIJIHJJJIJIGJJJHHHHHFFFFF@C@ 0 91:A>G -AMELIA:165:C03TUABXX:5:1101:11207:2960 + 1 568468 CGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCC @@@DDDD?DHAFDGD@F@<???DGCG3*/8=BB)@@F4=E;E>C>7=>B>C?;.7.;(;?;3?;=@<@730;53:4:>:&+2<<3:A>?<18>(:@3>CC> 0 -AMELIA:165:C03TUABXX:5:1101:9272:22713 - 1 568470 ACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCG ?DB>DDDDDDCDDDDDDDDDDCCCCDFCDDA>DB@DDFFFFFEHHEHJJJIHEJJJJIGFDIIGGIIHJJJJJJJJJJJJIHJJJIJJHHHHHFDFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:20222:8858 - 1 568472 TACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGAT BBDDDDDDCDDDDDDDDDDCDCDDCBDC>5?88DDEEEDFFFD@FHHGFHEJJIIIGGEJJJJHGBIIJJJJJIJJJIJJJJJIJJJJHHHHFFFFFFCC@ 0 1:G>A -AMELIA:165:C03TUABXX:5:1101:11994:45223 + 1 568475 GGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGA CCCFFFFFHHHHHJJJJJJJJJJJJJIJJJJJJJJIJJGIHIIJIJJJJJIJJJJJJHHHFFFDDDDDDDDDDDDDBDDDDDDDDCDDDDEEDDDDBDDDD 0 96:G>A -AMELIA:165:C03TUABXX:5:1101:5506:16169 - 1 568477 CGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAG B@@C@>>:44:(3CCACCDDC>4:>2<=BBBC@>>:A>3CDC;;..CAHC@;88;;66<B9B?>D>HGDFC?:CC@?>BDHH?@HGA2<C?HFDBDDB::: 0 6:G>A -AMELIA:165:C03TUABXX:5:1101:13408:18726 - 1 568477 CGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAG DDDDDDDDDCCCDCCDADDECEEC@?B=DDDDDECEECCEFDDDB?HHHHJHHCIJGIGIGIHJIIJJHJGJIGIJIJJJJJJJIIGGHHHHHFFFFF@CC 0 6:G>A -AMELIA:165:C03TUABXX:5:1101:16768:22431 - 1 568477 CGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAG DDDDDDDDDDDDDDDDDDEDCEDC>@>?BDDDDEDCEEEEFFFFECHHHHIIHFJJGJIHGJJJJJJJIJJJJJIJJJJJJJJJJIHGHHHHHFFFFFCCC 0 6:G>A -AMELIA:165:C03TUABXX:5:1101:14933:25341 - 1 568477 CGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAG BDDDDDDDDDDDDDCDCDDEDDCC@<>;DDDDDEDEEEAEFEFFD@HHHHIJHFJJJIGGHJIJJJJJIJJJJGGJJJIJJJJJJIHFHHHHHFFFFFCCC 0 6:G>A -AMELIA:165:C03TUABXX:5:1101:9923:39483 - 1 568477 CGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAG BDDDDDDDDDDDDCCDDDEDDEDC@BB;DDDDDEDEDEEEFFFFC?HHHHJJHFJJJIJIHJJJJJJIIJJJJJHJJJJJJJJJJIHGHHHHHFFFFFCCC 0 6:G>A -AMELIA:165:C03TUABXX:5:1101:19992:46646 - 1 568477 CGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAG BDDDDDDDDDDCDDCDDDDCCED@:?B=DDDDDECEECCEFFFEC;HHHHJJHFJJHGHEGJJJJJJIHJJIJJJJJJJJJJJIIHHEHHHHHFFFFFCCC 0 6:G>A -AMELIA:165:C03TUABXX:5:1101:2910:50680 - 1 568477 CGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAG DDDDDDDDDDDDDC?DDDEDCEDA:?@;DDDDDEDEEECEFFFFFCHHHHJJJHJJJIHGFJIJJJJJIJJJJJIJJJJJJJJJJJHHHHHHHFFFFFCCC 0 6:G>A -AMELIA:165:C03TUABXX:5:1101:19617:51214 - 1 568477 CGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAG DDDCCCA:DCA@CCDCCCAC@@@:4?>6DBBCCCCECC@DFFFFC?GHGHGGF@HGGG@BBIDIHIJIJJIJGHIGIIGGGJJIIHGCHHHHFDFEDFCCC 0 6:G>A -AMELIA:165:C03TUABXX:5:1101:17241:52321 - 1 568477 CGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAG DDDCCDDDDDDDDDCDDDEDCDDC:@B?DDDDDEDEECCEFFFFD@HHHHIIHGJJJJJIGJIIJJJJJJJJJIHIJJIJJJJJJIHGHGHHHFFFFFCCC 0 6:G>A -AMELIA:165:C03TUABXX:5:1101:2961:64180 - 1 568477 CGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAG BDDCDDDDDCDDDDCDDDEEEEDC@?B@DDDDDEDCEECEFFFEC@GHHHHGIHJIIJJHGJJJJIJJJJJJIJIIJJJJJJJJJIHBHHHHHFFFFFCCC 0 6:G>A -AMELIA:165:C03TUABXX:5:1101:9899:49854 - 1 568480 ACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCC CDDDDDDDCCC<CCDDDCEEC@BB9DDDDCEDDDDCDEDEEC?FFFHHGHDJIIHHEDJGJIIJIIJJIJIIJIJJJJJJJJJIHJJIHHHHHFFFFFCCC 0 9:G>A -AMELIA:165:C03TUABXX:5:1101:10454:18520 + 1 568481 CTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJIJJJJJJJJHHHFFFFFDDDDDDCDDDDDDCDDFFEEDDDDBDDDDDDDDDD 0 90:G>A -AMELIA:165:C03TUABXX:5:1101:19791:51681 + 1 568481 CTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJHHHFFFFFDDDCDDDDCDDDDCDDFFEEDDDDDDDDDDDDDDD 0 90:G>A -AMELIA:165:C03TUABXX:5:1101:10221:42612 - 1 568482 TAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCC >CCCDDCDCACCDCB@DDC:B><DDDDDEDDDDCDDDDC@@DDCDBFB?HHIG@F=FDIGGHB8IHGAGBGFJIIJIJJJGHGIEJJJHHGGHFFFFFC@C 0 11:G>A -AMELIA:165:C03TUABXX:5:1101:12096:40234 - 1 568483 AATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCG DDDDDDDDCDCDDDCEECCDBBDDDDDEDDDDCDDEDDDCDDDDDDDBDDDDDD@FFHHHHJIJJJJHHJJJJJJJJJJJHGIIJJJJHHHHHFFFFFCCC 0 0:A>G,12:G>A -AMELIA:165:C03TUABXX:5:1101:3042:49725 + 1 568486 CTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTC CCCFFFFFHHHGHJJJJJJJJJJJJJJJJJJJJJJJJJIJJIFIJJJJJJJIJJJHHHHFHFFFDDEEEDDDDDC<CDFEDDDDDDDDDDDDDDDDDDDDD 0 85:G>A -AMELIA:165:C03TUABXX:5:1101:21186:2353 - 1 568489 CAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTA ###?CC>B>C>::<)0@BBAA>@4>:3C@4ACC:BBBBB@>A?9A?97)E<EGGIGG@7<BBIEIGIHDEGBB9D:D0IFBGCIGEIIHDDBBDDADF@<@ 0 18:G>A -AMELIA:165:C03TUABXX:5:1101:11191:4415 - 1 568490 AACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTAT CCCCDEEDDDC>DB>DDDDDDDCDDCDDEDDCADDDDDDDBDDDDFECHHHHJGJIIIJIGGIJJJJIJJIIHGDIIJJJJJJJJJJJHHHHHFFFFFCCC 0 19:G>A -AMELIA:165:C03TUABXX:5:1101:7862:28687 - 1 568494 CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATA CDC@DC>:8&0DDBDDDCCCCCA>3CC:4ABBDBBDBBDDDC@7FFHEHHD=HEF@@CGIGIIJGGHECD8BIIIHGIGIGHFDBGHGFHDBFFFFDD?@@ 0 23:G>A -AMELIA:165:C03TUABXX:5:1101:18174:59520 - 1 568505 CCCCCATTATACCTAGAACCAGGCGACCTTCGCCTCCTTGACCTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACA ##################################################EC=)F=8..(@0GDAG>GF?1)AGF>GF=FGGICJJIIHHBDHDDA=4111 0 34:G>A,58:G>C,68:A>C,71:G>T,90:T>A -AMELIA:165:C03TUABXX:5:1101:19743:34362 - 1 568507 CCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAG ?>4:>:4:3A@:>>>:B<<8>5@<=B@:>;(BCCCCEDD?7)7>ACDC2GGHD@F@8(EGIIHGDAGB;?BEF@C:AA=HHIHBGC<GDD?DHDA?AD?@? 0 36:G>A -AMELIA:165:C03TUABXX:5:1101:20558:4348 + 1 568513 ATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCT CCCFFFFFHHHHHJJIJJJJJJJJJJJJJJJJJJJJJJJIIJJHJJJDHIEIHHHHHFFDDDEEDDDDDDDDDEEDDDDEEEDEEEDDEDDDDDDDDDDDD 0 58:G>A -AMELIA:165:C03TUABXX:5:1101:10722:52200 + 1 568516 CCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGC CCCFFFFFHHHHHJJJJJJIJJJJJJJJJJJJJJJJJIJIJJIJGHIHIJJJHHHFFF=CEECDDDDDDDEECDDCFFEDEEEDDEDDDDDDDDDDDDDDC 0 55:G>A,99:A>G -AMELIA:165:C03TUABXX:5:1101:4465:6081 + 1 568517 CTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCA CCCFFFDFHHHGHJJJJJJJIJJJJJJJJJJJJJIIIIIJJIJFEHHHJJJJHHHFFFEEEEEDDD?CDFECDDDDDEDEEEDDDCDDDDDDDDBDDDDDC 0 54:G>A,98:A>G -AMELIA:165:C03TUABXX:5:1101:19822:44302 + 1 568517 CTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCA @@@FFFFFHHHHHIJJJJJJGJJJJJIJIJJHIJJJJJJGIFI@GHGAGHJJHHHFFFDEEEEBDDDDDEECDCCDCDDEEEDDEDDDCDDDDDDDDBDDC 0 54:G>A,98:A>G -AMELIA:165:C03TUABXX:5:1101:6963:44960 + 1 568517 CTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCA @CCFFFDDFFDDHIJGGIGG@FDGIGBGCHGFHGJEFFFDGAF8=D=@E@CEEHABB?C>;>BBBB;CDEE:?ACBC@C>BACDDCCC>@BDCBDD?BDDC 0 54:G>A,98:A>G -AMELIA:165:C03TUABXX:5:1101:15438:58349 + 1 568517 CTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCA CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJIJJJJJJJJIFHIIIJJJJHHHFFFEEEEEDDDBDDEECDDDFDEDEEEDDEDDDDDDDDDDDDDDDC 0 54:G>A,98:A>G -AMELIA:165:C03TUABXX:5:1101:13150:61331 + 1 568518 TAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCAC CCCFFFFFHHHHHJJJJJJIJJJJJJJJJJJJJJJIJIHIIJCHIIIJJJJHHHFFFFEEEEDDDDDDDE:?ACD@CCEDEDDCCDDDDBDCBBBDDCADC 0 53:G>A,97:A>G -AMELIA:165:C03TUABXX:5:1101:10092:4251 + 1 568520 GAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTC C@@FFFFFHHHHHGIIIJJJJJGJJJIIEDHIGHIGEHGJGGGCFHIIJJIHEFFFFFEEDDDDDDEEDDDDEEDCADDDCEDCCCDDDDDB@<?CCCDDD 0 51:G>A,95:A>G -AMELIA:165:C03TUABXX:5:1101:1583:38485 - 1 568525 AGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAG BDDDDDDDDDDDCDDDDDDDDDDEDCDDDDDDEEEEFFEBFHHJJJJIJJJJJIJJJJIGIJJIJIJJJJJJIIJIHHHIJJIHJJJJHHHHFFFFFFCCC 0 10:A>G,54:G>A -AMELIA:165:C03TUABXX:5:1101:12337:48423 - 1 568528 CGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTG DCBDDDDDDCDDDDDDDDDDEEEDDDDDDDEEEEEEBFFHHJJJJJJJJJJJJJJJIJJJJJJJJJJJIJIJJIIHJJJIIJJJJJJJHHHHHFFFFFCCC 0 13:A>G,57:G>A -AMELIA:165:C03TUABXX:5:1101:12333:48844 - 1 568528 CGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTG DDBDDDD>CCDDDDDDDDDDEEEDDDDBDDEEDECCDFFHHJJIJJIJJIJJJIJJIJJJJJIJJIIJJIIHEHHHJJJIHJJIJIJIHHHGHFFFFFCCC 0 13:A>G,57:G>A -AMELIA:165:C03TUABXX:5:1101:12326:22613 - 1 568530 ACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTC DDDDDDDCDDDDDDDDDDEEEDDDDDDDDDEEECADFFHHJJJJJJJIJJJJJIJJJJJJJJIGJJJIIJIGHFIJIHGJIJJIIJHGHHHHHFFFFFCCC 0 15:A>G,59:G>A -AMELIA:165:C03TUABXX:5:1101:15411:6227 - 1 568533 TGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCC BDDCADDDDDDDDDDEEEDBDDDDDDDEEDC@DDDFFHHHHHHJJJJJJJJJJJJJJJJJIJIJJJJJIIHJJJIGJJJJJJIHEJJJHHHHHFFFFFCCC 0 18:A>G,62:G>A -AMELIA:165:C03TUABXX:5:1101:13970:47024 + 1 568535 CGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCAC CCCFFFFFHHHHHIJJIJIJIJJJJFHJIGIIJJJJIJJIJJJJJJJJJJJJJHIIJJJJJJHHHHHHHFFFFFEDDDDDDDDDDDDDDDDDDDDDDDDDD 0 36:G>A,80:A>G -AMELIA:165:C03TUABXX:5:1101:10582:41984 + 1 568536 GACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACA CCCFFFFFHHHHHJJJJJJJJJJJHHIGIIJJJJJIJJJJIJIJJJJJIJJIHIJJIJJJJJIJHHHHHHFFFFDDDEDDDDDDDDDDDDDDDCDDDDDDB 0 35:G>A,79:A>G -AMELIA:165:C03TUABXX:5:1101:3639:25442 - 1 568548 TTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAA @EDCBCCBBCCAA>:929;B=BCCCA?A?8EDHEEC=;GGFGHFGGIIIHF=DD<BHGFFDD:??4<?CCCHFC9IGGIHC+HAECAEFFCBBDBBDD@@@ 0 33:A>G,77:G>A -AMELIA:165:C03TUABXX:5:1101:17363:4229 + 1 568559 GTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAAT @@@FDDDDHGHGFGEHJIIGFGIGJJIGC?DEDFCFGGJIGCHGHIBHDEHB;FCG@CHHID@DACEHFFFF@DE>AA>AA@CACDDA>@C@BCCDCCCCC 0 12:G>A,56:A>G -AMELIA:165:C03TUABXX:5:1101:7121:26502 - 1 568559 GTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAAT DDDDC@BBBDBDDCCDDDBB@DCFFFFHHFHFHGIJIJHGIIJJJJIIJIGHJHEGHGDGGIHGJJJIHHHDIHHDHHDHIIIJIGJJHHHHHFFFFFCCC 0 44:A>G,88:G>A -AMELIA:165:C03TUABXX:5:1101:6780:12951 - 1 568563 TACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCC DBDDBDDDDDCDDDDDDCEEDBBFEDFEHHHHFEHEJIHGCIIGHDIGIJIIGGHIIJJJIGGGIHEJIIGEJJIGJJIJGIJIIHEHHFFDHFFFFFC@@ 0 48:A>G,92:G>A -AMELIA:165:C03TUABXX:5:1101:6966:48733 - 1 568563 TACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCC C?DDDDBADCDCDDDBDAC@A?BBFEFGHHFEGHFIGHAE@CGHBCJJJIHGGFBIIJJJIIGGGIIGIIIHGIEIGIJHGIGIIHFJHHHHGFFFFF@@B 0 48:A>G,92:G>A -AMELIA:165:C03TUABXX:5:1101:12025:55524 - 1 568565 CTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGG 9;>?>>:3><>95=7;>>==;AA@@>B?@DB>A?CC>=7DDDA@A=8=8))8BB*??38<D;B@1AD@C9EE>EEDF?A;IEIIIEEEDDDDDA<8DD??= 0 50:A>G,94:G>A -AMELIA:165:C03TUABXX:5:1101:2169:60119 - 1 568572 TTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTA DDDDDDDDDDCDD@?DEEFFEDDDCAEDCDDDDDCAAFFFHHHHHHGIJIJGGJJJIGIJIGGIEJIGGJIIIIJIGFIIGIGIGIHBDDHHFFFFFFCC@ 0 57:A>G -AMELIA:165:C03TUABXX:5:1101:3263:60291 - 1 568578 CCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAG BBBC?AA?BCDDCC@CEEEDCCC@@:CBCB?BACCEEDC?EEHFEHHIGIIIGHHFBGIIIIGIIIIIIHF?GIHFFBGHG@GGIIIGFDF8HDDDAD@@@ 0 0:A>G,63:A>G -AMELIA:165:C03TUABXX:5:1101:19083:24675 - 1 568580 CCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC DDDDDDDDEEEEEEFEEEEDEDCCDBC>=DDEEEDECCFFFFFHHHHFIHJIIIHJJIJJJIJIJJJJIHJJJJJJJIGHGIJJJIHJHHHGHFFFFF@?B 0 65:A>G -AMELIA:165:C03TUABXX:5:1101:3396:41674 - 1 568580 CCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC BDCA??@DDEEEDDEEEEFDEDDCDDDDDDDEEEECCBFFFFFHHHHHIHJGFHEJJIIGIIGIIJIHD>GIGIIIJJIIIJJJIHHDHGGHHFDFFF@@B 0 65:A>G -AMELIA:165:C03TUABXX:5:1101:11243:31371 - 1 568583 ATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCAC DDDDDEDDEEEDDDDDCCCCCDDDDDBCCCDDDC@EEEEFFFFFFHHIIIFCJJIHHBIEGIJJJJJHIIJIIIIHGGIIHGGHIJJIFGHHGFFDDFB?@ 0 68:A>G -AMELIA:165:C03TUABXX:5:1101:16672:3690 - 1 568589 ATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCAC DDDCDCDDCCDCCC>C@?=8BC?CCCC@;DCCACC@CBD=5B;;CD@GIIHGCGGGIHHGBIIHEHFDBF@DIIIGHGDFBFGHIHBHFCCAFBDD?B@@? 0 74:A>G -AMELIA:165:C03TUABXX:5:1101:17259:45874 - 1 568589 ATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCAC EEEEEEEEEEDEDDDDDDDDDDDDCCDDDDDDDDEEDDDEEHJJJIJJJJIJJJJJJJJJIJJJJJJJJJJIJJJJIIJJJJJJJIJJHHFHFDFFFDBBB 0 74:A>G -AMELIA:165:C03TUABXX:5:1101:4895:54966 - 1 568589 ATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCAC EDDDEDDDDCCDCCCDDDBBDCCCCACDCCACC@EDDDDB?EIHEAIIIJJIGGBCJJIHFGIGIGGGHD@DIIHEIGIGIIJJIIGJHHFHHDFFFD@@@ 0 74:A>G -AMELIA:165:C03TUABXX:5:1101:3629:60053 - 1 568589 ATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCAC DEEDEEEEEEDEDDCDDDDBDDDDDCDC@C>DDEEEDDFE@HJJJIJJJJJJJJJJJJJJIJJIJJJJJJIHJJJJJJJIIIHJJIJJHHHHHDFFFFBBB 0 74:A>G -AMELIA:165:C03TUABXX:5:1101:19989:25964 + 1 568923 CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAATTACCACCTACCTCC ???D;:D;DDDD?<+CEI=3A;E<<4C<11C@B*:6)0*9***9D8BDEI)8=<88)/8787;((((7)..?A9;?@;@;>A@################## 0 17:A>G,80:T>C -AMELIA:165:C03TUABXX:5:1101:6773:27426 + 1 568948 TAATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACCACCTACCTCCCTCACCAAAGCCCATAAAAATAAAA BCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJIJIJJIHHHHFFEFFEEDEDDDDDDDDDDDDDDDDDDD 0 55:T>C,61:T>C -AMELIA:165:C03TUABXX:5:1101:19247:10831 + 1 568950 ATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACCACCTACCTCCCTCACCAAAGCCCATAAAAATAAAAAA CCCFFFFFHHHHHJJJJJIJIJIIJJJJJJJJJJJJJJJJIJJJJJIJJIJJJJJJIJJIIJIJIIIJEEHGHFFDDCCEEDDDDDDDDDDDDDDDDDDDB 0 53:T>C,59:T>C -AMELIA:165:C03TUABXX:5:1101:2847:25911 + 1 568950 ATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACCACCTACCTCCCTCACCAAAGCCCATAAAAATAAAAAA CC@DFFFFHGHGHIJJJJJJJJJJJJJJJJJJIGHIJJJJIJJJJJJJJJJJJJJJJJJJJJIJJJJGHHGHHFFBEEDDEDDDDDDDDDDDDDDDDDDDD 0 53:T>C,59:T>C -AMELIA:165:C03TUABXX:5:1101:11148:5717 + 1 569014 ACCTACCTCCCTCACCAAAGCCCATAAAAATAAAAAATTATAACAAACCCTGAGAACCAAAATGAACGAAAATCTGTTCGCTTCATTCATTGCCCCCACAA BCCFFFFFHHHGHJJJJJGJJJJJJJJJJJJIJJJJDHIIJJIIGJJJJJJJJJJJJJJJJHHGGHFFFFDDDDDDDDDDDDDDDEFEEEEDDDDDDDDDB 0 37:C>T,79:A>G -AMELIA:165:C03TUABXX:5:1101:17492:53844 + 1 569016 CTACCTCCCTCACCAAAGCCCATAAAAATAAAAAATTATAACAAACCCTGAGAACCAAAATGAACGAAAATCTGTTCGCTTCATTCATTGCCCCCACAATC BCBFFFFFHGHHHJJIIJJJIJJJJIJJIJJJIJJJJJIJIIJJJJJJJJGJIJIJJJJJJGGHHHFFFDEEEEECDDDDDDDEEEEEEDDDDDDDDDDDD 0 35:C>T,77:A>G -AMELIA:165:C03TUABXX:5:1101:7094:46607 + 1 569024 CTCACCAAAGCCCATAAAAATAAAAAATTATAACAAACCCTGAGAACCAAAATGAACGAAAATCTGTTCGCTTCATTCATTGCCCCCACAATCCTAGGCCT CCCFFFFFHGHHHIJJJJJJIIJJJJJJJIJJJJJJJJJJJJIGIIJJJJJIJJGGJIJJHHHHFFDFFFDDDDDDDEEEEDDDDDDDDDDCDDCCDCCDC 0 27:C>T,69:A>G -AMELIA:165:C03TUABXX:5:1101:13269:30787 + 1 569039 AAAAATAAAAAATTATAACAAACCCTGAGAACCAAAATGAACGAAAATCTGTTCGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACT CCCFFFFFHHGHHJJJJJJJIJJJJJJJJIJJJJGHJJIJJJIJJJJIJJJGJJJJGJHHHHHHFFFFFFDDDDDBDDDDDDDDDDDDDDDDDDDDD@CDE 0 12:C>T,54:A>G -AMELIA:165:C03TUABXX:5:1101:11366:14634 + 1 569046 AAAAATTATAACAAACCCTGAGAACCAAAATGAACGAAAATCTGTTCGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATT CCCFFFFFHHHGHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJIJJJJHHFFFFEEEEECDDDDDDDDDDDDDDDDDEDDDDEDE 0 5:C>T,47:A>G -AMELIA:165:C03TUABXX:5:1101:6815:4817 + 1 569080 CGAAAATCTGTTCGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAAT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJIIJJIJJJJJJJJJJHGFBEEEEEEEEDEEEDEEEEECDDDDDDDEDDEDDDDDDDDDDD?CC 0 13:A>G -AMELIA:165:C03TUABXX:5:1101:16417:15341 + 1 569080 CGAAAATCTGTTCGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAAT CCBFFFFFHHHHHJJJJJJJJJJJJJIJJJJEHIGIJJHJJJJJIJJJJJJIJHGFFFEEEEEEDCDEEDEEEFEDDDDDDDDEDDEDDDDDDDDDDCDDD 0 13:A>G -AMELIA:165:C03TUABXX:5:1101:14956:37964 + 1 569080 CGAAAATCTGTTCGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAAT CCCFFFFFHHHHHJJJJJJJJJJJJJIJJJJIIJJJJJIIIJIJJJJJJJJJIHGFBDEEEEEEEDEEEDDEEEECDDDDDDDEDDEDDDDDDDDDDD?CD 0 13:A>G -AMELIA:165:C03TUABXX:5:1101:1557:58056 + 1 569080 CGAAAATCTGTTCGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAAT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJHHFFFEEEEEEEDEEEDEEEEEACDDDDDDEDDDDDDDDDDDDDDDDC 0 13:A>G -AMELIA:165:C03TUABXX:5:1101:2531:30088 - 1 569083 AAATCTGTTCGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATC C>:9CB>>?>@A@C@@C@C??BBB@@<4+<A>:?<A>@>?870CCCFHHGIIIIGIIIIIGHDIGIGGDBIIIGFAIGIHEFGGGGHE<HHFHFFFDA@?@ 0 90:A>G -AMELIA:165:C03TUABXX:5:1101:5810:51274 - 1 569083 AAATCTGTTCGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATC AC@CDDDDBDCCDDDEDCDDDDBDDBBCADDC@DDCCDDDB<5DDDFHHJJJJJJJIJIGJIIJJJIIIGJJJJIHJJJJJIJHJJIHDHHHHFFFFFC@C 0 90:A>G -AMELIA:165:C03TUABXX:5:1101:6382:5977 - 1 569087 CTGTTCGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCAT ?<>5854AACCDC>C><5<BBBBCCA>:3CC>@CCBB;5BB?CEHFHHHDCHCGIGIIIIHCGB:>EGEFGGGHHIGHDFGGFC2AGGHHHHFDD;D?@@? 0 94:A>G -AMELIA:165:C03TUABXX:5:1101:20152:20663 + 1 569087 CTGTTCGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCAT CCCDFFFFHHHGHJJJJJIJJJJJIIJIIJJGIJGIJIJIJEHGIJJJJHGHHHEFFFFFFDDDEEDDDDDBDCCCDCCDDDDDDDDBDDCCDDDEEEDDE 0 6:A>G -AMELIA:165:C03TUABXX:5:1101:11967:19233 + 1 569089 GTTCGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCA B@@FFFFFHHHHHJJIGIJIGIGJIEGIJGEIIJIIJIJGIJJJIGG@EHGHHGHFFFFFFFEEECCEDBB?CDECDDDDDDCDDDDDDCCDDDEEDDDDC 0 4:A>G -AMELIA:165:C03TUABXX:5:1101:19300:31384 + 1 569089 GTTCGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCA CB@FFFFFHHHHHIJJJJJJJJJJJIJJJIIJJJJIJJJJJJJIJJJGHHHHFGHFDFFFFFEEDEEEDDDCCDEDDEDDDDDDDDDDDCDDDEEEDDEDC 0 4:A>G -AMELIA:165:C03TUABXX:5:1101:17118:59817 + 1 569091 TCGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAAC CCCFFFFFHHHHHJJJJJJJIJJJJJJIJJJJJJJJJJIJJIJGIGIIIJIIHGHHHHGFFFFFFEDDDDDDEDDEDDDDDDDDDDDDDDDEEDDCDDDDC 0 2:A>G -AMELIA:165:C03TUABXX:5:1101:6034:13000 - 1 569092 CGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACA BACDCCDC@CCBDDDDDBA>CA::C<>:AABB85BDDBDFFFFHFHHHGHGEJJHIIIGG@IIIIHIJGIJJIJIIIHDCIJGJJIIHFHHHHFFFFD@@@ 0 99:A>G -AMELIA:165:C03TUABXX:5:1101:19996:10934 - 1 569094 CTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAAC DDDDCCADC@9>DDDBC>CCAADCA@CCB@;5DDDDDDFFFFFCHHHFHFGIHIJJIGGJJJIHGHGGJJIIFJJIGCJIGJJJJIHHFHHHHFFFFFBBB 0 -AMELIA:165:C03TUABXX:5:1101:4373:19050 + 1 569094 CTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAAC ??@FFDDFAFDFDCGBF@CEBFHEHICHCCGGGHHGGD?@:;;CD7)=CE@=EEHCHFHEEADB<<;@CC:>5;>;?<@@BBBCCCCCCDDCCDCCCC@BB 0 -AMELIA:165:C03TUABXX:5:1101:9975:62295 - 1 569094 CTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAAC @:@4+:@:(25>BBB@:4C:>CCCA>:3@<0&BBBBCCEDCEEBHHHFFHFGHEEGCF;IGGHDAHGGDHF?EIIIGGCHHEDAFE<C:F?HDD;DAD@@@ 0 -AMELIA:165:C03TUABXX:5:1101:13694:16224 - 1 569098 ATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGAC CA@CC?<BCBBBA>A:4(CA9@C@<<59BBB?BBCCDCC@CACCEFD@BD>ECA>IIIFF@GHHBCHF7IHE?1GHDHIHHF>FAF>HDDFGHAD@FD@?@ 0 -AMELIA:165:C03TUABXX:5:1101:15895:17465 - 1 569098 ATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGAC @>>CC?5-@<<@CCCAA:C??AC>8<5&DDDDDDCDDCAAEECACEFFFECEEA8GGHHD@D?GEEG@?FBB?:DCCGIHHHDHDF>GFBDHHFD@FD@=? 0 -AMELIA:165:C03TUABXX:5:1101:1650:30462 - 1 569099 TTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACT :ADDDDDDDDDCDDDCADDDDDCB<90DDDDDDDDEDDDDEEECEDFFFFFFEBIGIIHEGDJIGJJHJIHGCIIGJJIIHGGHJIJJHFHHHFDFFFBC@ 0 -AMELIA:165:C03TUABXX:5:1101:12365:35968 - 1 569101 CATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAA 39?9BBBD?C@DC@>DCCCCA<?90B>;DDDCCC@>3CEDCCECC@CDFFFEGIIJHGHGFHDFD=GGGHGFBEGIIHHDGEIIIHGIHHDHFFDDDF@C@ 0 -AMELIA:165:C03TUABXX:5:1101:6791:11071 - 1 569103 TTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATC BB@DDDDC>DCC@DDDDDCD@>9DDDDDDDDEDEDDEDDDCEDECDEDFDHJJJIIJIJIJJIGJJIGCJHGJJJJIIIFIHJJHJJJHHHHHFDFFFCBB 0 0:T>C -AMELIA:165:C03TUABXX:5:1101:4491:45424 - 1 569103 TTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATC ?9<DDDDCACC@@DDCDDDDD@9DDBBDDDCEDECDEDDDDCEDEEECEBHJJIIGHEEHJIHBJIHD:JHHJJJIIGHDHGJIGJIHGHHHHFFFFFC@C 0 0:T>C -AMELIA:165:C03TUABXX:5:1101:14051:9108 - 1 569105 GCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCAC @DDDDCACDC@DDDDDCDB@5DDDDDDDDEDDEDEDDDDDDEEEEDCBHJJJJIJIJJJJIHJJHH?JIGJJJJJJJIJIJJIJJIJHFHHHHFFFFFBBB 0 2:T>C -AMELIA:165:C03TUABXX:5:1101:2614:13945 - 1 569106 CCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACC BBB@@><CC>CC>C><BB9@BBB>BBCCA@CCCA@<CCC@@A@CCCBHIIHG@B=BCGGD;GIHHDBGGGHCFE>HHFFFH>GEFHGGFHDAFDDDAA+@? 0 3:T>C -AMELIA:165:C03TUABXX:5:1101:20887:4345 + 1 569107 CCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCA @@<D<DBDFBDFDDHI3<A@CFFBCFH0809?D8*9??8BCAH<==9=F>ABAGEECC:3.?7;)99>>?(5;@>;CCCC@CCCCA@@<@@B59>:A:A?3 0 96:T>C -AMELIA:165:C03TUABXX:5:1101:11790:4282 + 1 569109 CCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACC BCCFFFFFHDFHHJJJJJJIJIJJJJJHIJIIJJIGIIHJGIIGIGJJJJJJJJJJIHHGHHFFFEEDEEDDDEEDDDEDDDDDDDDDDDDDDDDDDDDDD 0 94:T>C -AMELIA:165:C03TUABXX:5:1101:7754:23799 + 1 569109 CCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACC =;???DBDH8BFFE?EGIIIBGGIBAF0???FB?B@FE@H4DGDCE<BFAFGGHIHC?==7;?B<B;;;>>5;;;3>CC:;5<C?B8555>5C>:>:<@AB 0 94:T>C -AMELIA:165:C03TUABXX:5:1101:5473:24108 + 1 569109 CCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACC @C@FFFFFBFHDFHIGIIGGHIJFIJB6BGIICBE4?FHGB@FGGGJGIJJGIIIGIBHFHF;BDEACEEDDCDDCCCDDD>CCBDD>>BB>CDCDDDDDD 0 94:T>C -AMELIA:165:C03TUABXX:5:1101:20185:52249 + 1 569109 CCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACC CCCFFFFFHHGHHJJJJJJJJJJIJIJIIJJJJJJJJJJJIIJJJJJJJJIJIJJJJHHHHHFFFEEDCC@CCEEDDDEDDDDDDDDDDDDDDDDDDDDDD 0 94:T>C -AMELIA:165:C03TUABXX:5:1101:16378:63089 + 1 569109 CCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACC CCCFFFFFHHHHHJJJJJJJJJJJJIJHIJIJIJJJJJJJIJJIJJJJJJJJJJJJIHHHHHFFFEEDAEDDCDEDDDDDDDDDDDDDDDDBDDDDDCDDD 0 94:T>C -AMELIA:165:C03TUABXX:5:1101:2948:11177 + 1 569110 CACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCC C@CDFFFFHFHDFGGFGEHIEFHGGIIGEIHHHIGDIIIJIIJIGIGHG>EGIG;;=CAEE>BDF@ACEEDDEEEC>CCCDDCBB@?<<><@CDDCC><?9 0 93:T>C -AMELIA:165:C03TUABXX:5:1101:16866:5557 - 1 569111 ACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCG 4DDC@DDCCCDDB@9DDDDDDDDEDDDDEDDDDDDDDDDDD@DFFHHHHHJJJIIHJJHF?JIGJIJJIIIHIIJJIJJJJIHJIHGJHHHFF@DDBFCBB 0 0:A>G,8:T>C -AMELIA:165:C03TUABXX:5:1101:15344:54384 + 1 569113 AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAAC C@@FFDDDHHHHHJJGIIJJIIEHJJIJEHGIJIGGGGIJIHIJJGIGGIJIIGJEHGHHHFFFDEEEEDEECCDACCDDDDDDDDDDDDDDDDDDDDDDD 0 90:T>C -AMELIA:165:C03TUABXX:5:1101:19377:17051 + 1 569116 CCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAAT @C@FFDDDAFHBHIJJGIIIBCFIIGIIIIGIICGIGIIIIIIIIHGIDHIEHHJCEH?EBB>CD>CBDEAC@@>?CABDDDDDBDC@CCAABB>@BB?<C 0 87:T>C -AMELIA:165:C03TUABXX:5:1101:19821:23266 + 1 569116 CCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAAT ??@DDDDDCFFHDHGBFHGHHGFHIHFGDFEGC4DB?FDFIIGEIGAGAGFCGHIGEHHHFC;CBACCFAADDCCCCBDD==BDDCCDDDDCDDBBDBBBD 0 87:T>C -AMELIA:165:C03TUABXX:5:1101:21308:61312 + 1 569116 CCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAAT CCCDFFFFGHFHHIJJFIIIIIGIIGI>@GGHGGIJJIJJIJGGGIGGGEGHIGGHHHHHEEDEFCADEDCC;@CCCDD?BBDDBDDCDDDDBDBBBBDBD 0 87:T>C -AMELIA:165:C03TUABXX:5:1101:10183:11317 - 1 569119 AGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGAC CCCD?<5DDDDDDDDEDEDDEDDDDCDCDDDDB@DDDEEFFFFFFHHEJJIGBJHGJJJJIIIHHIJJHJJJJJHJJIHJJJIGGCHEAHHHHDFFFFCBC 0 16:T>C -AMELIA:165:C03TUABXX:5:1101:10145:21000 - 1 569119 AGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGAC CCC@@99DDDDDDDDEDDCCEDDDCCCCDDDB@;DDCCC@DEDDBEHEIGEF<JIHJJIIGFFBGFJIIIIHIHDJHHGIIIGGHGGHDHHHHFFFFDCBB 0 16:T>C -AMELIA:165:C03TUABXX:5:1101:5404:3885 + 1 569126 CCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACTAATCAA @@@FFFFFGHFHHIJJJJIIJJJHIJJJJJJJGIIJJGGJJJJJJJJJIJJJIGIIJJGGGHGHGFFEEDCDDDBCCDDDDDDDDDDDDDDCDCDDDDDDD 0 77:T>C,99:C>A -AMELIA:165:C03TUABXX:5:1101:4102:24393 - 1 569128 CGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACTAATCAAAC DDDDDDEDEEDEDDDDDDDDDDDDBDDDDDDDEEEECD?FHHE;IHFJJJJIJJHJJJIFJIJIHGJJIIJIJIHF@HFAJJJJJIJIHHHHHFFEFFCB@ 0 3:C>A,25:T>C -AMELIA:165:C03TUABXX:5:1101:6523:35689 - 1 569128 CGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACTAATCAAAC DDDDDDEDEEDEDCCDDDDDDDB@<DDDBCCCEEDEDFEFHGGCIG>JJIJIHGEIGIGGIJJJIIJJIJJIJIHGCHGCJJJJIJJJHHHHHFFFFFCBB 0 3:C>A,25:T>C -AMELIA:165:C03TUABXX:5:1101:16375:62374 - 1 569128 CGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACTAATCAAAC BDBBDDDDDCCEDDDDDDCDC@A5@DDDDCCDDEECCBBFHHE@IHCJJJIHCHCF>GECIGIIHFIIGJIGIF@FCFF<HHEGEJJIFHFHFFFFFFC@@ 0 3:C>A,25:T>C -AMELIA:165:C03TUABXX:5:1101:16624:38360 + 1 569136 TACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACTAATCAAACTAACCTCA CCCFFFFFHHHHHIJJJJJJJJIJJJIJJJIIIJJJJJGIJJJJIJIIJJJJJJJJIIGIIJJJGHHHFFFFFDDDDDDDDDCDDDDDDDDDDDDDDDDDD 0 67:T>C,89:C>A -AMELIA:165:C03TUABXX:5:1101:13247:37816 - 1 569139 TGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAA EDCDDDDDDDDDDBDDDDDDDEDDDBDBDDFD?FHFHHHJJJJGJHJJIJIHIHFJIGGIGIGEG@HGEJJJJJJJJIJJJJJJIJJJHHHHDFFFFFCCB 0 14:C>A,36:T>C -AMELIA:165:C03TUABXX:5:1101:14921:47704 - 1 569140 GATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAAC DDDDDDDDDDBB>DDDDDDDEDDDBB@DDDD?FEEHHHHHIGHHGJJIIIIIHFJIGFIGIHFF?HF?JJJJJJJJJJHJJJJIJJIHHFHFHFDFFFBBB 0 15:C>A,37:T>C -AMELIA:165:C03TUABXX:5:1101:15192:8191 - 1 569143 CATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCACCAACAACCGACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAA DDCDDDDDDBDDDDDDDEDDDDBBBBDB=DEEFFFFFFHEHAC;(IHGIHAJJIHJIIHFF?HH?JJJJIJJJJIHJIJIIJIHFHIHFHHHHFFFDFCCC 0 18:C>A,40:T>C,56:T>C -AMELIA:165:C03TUABXX:5:1101:18354:56132 - 1 569143 CATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAA CDDDDDDDDBDDDDDDDDDDDDDBDBDC?FEBFFHHHGGHHEIIHJIGIHFJJIIIIIHDG@GD:IJJJJJJIIIJJIJJGJIIHJGHFHHFHFFFFFCCC 0 18:C>A,40:T>C -AMELIA:165:C03TUABXX:5:1101:13210:64272 - 1 569143 CATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAA @>CCACAA<9BBBBCCA;CCBB?<?;?=5EB:FFEC=??:ED@C=GHEIGIHIGDEHGB<D:GDFIIIIIGGHGHCHCGGGIHHEFCA<F;=HDDDAF@@? 0 18:C>A,40:T>C -AMELIA:165:C03TUABXX:5:1101:9865:24763 + 1 569144 ATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAAT @@CFFFFFGHGGHJIJJIEHGIJGGIGHJJJIJJJIJII@HIJIB@FHHHGEGDHIIJGGHHFHEEFFF=?=;@CDDDDCCCCCDCDDDBCCDDCDDB@BD 0 59:T>C,81:C>A -AMELIA:165:C03TUABXX:5:1101:2591:43602 + 1 569144 ATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAAT CCCFFFFFHHHHHJJJJJJJIIJJJJJJJJJJJJIIIIJJJJJJJJGJJJIJJJJJJIIJJHHHHFFFFDDDEDDCDDDDDDDDDDDDDDDDDDDCDDDDD 0 59:T>C,81:C>A -AMELIA:165:C03TUABXX:5:1101:14644:54713 - 1 569144 ATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAAT CDDDDDDB>DDDDDDDEDDBBBBDDCA=DFDFFFHGHFFHHJIIJIIIHAJIHHJIIHEG@GC:JJJJIJIJIHHJJJJIJJIIJIIHHHHHHFFFFFCCC 0 19:C>A,41:T>C -AMELIA:165:C03TUABXX:5:1101:8669:62553 - 1 569144 ATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAAT CDDDDCBB<DBBDDCDDDDDDB?D@A?;DFDFFFHHHCHHCGIGGHFJIGIJIIJIHGFD?GD?JJJIGJJIGHFIIIHFJIHFHEHHHHFHHFFDFF@@@ 0 19:C>A,41:T>C -AMELIA:165:C03TUABXX:5:1101:17846:25975 + 1 569147 CTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAATGAT CC@FFFFFHHHHHJJJIJJJJJJJIJJJJJJJJIJJGIIJIJJJJJEDGHIJJJJJIJJJHHHFFFFDDEEEDDDCDDDDDDDDDDDDDDDDDDDDDDDDA 0 56:T>C,78:C>A -AMELIA:165:C03TUABXX:5:1101:9275:24199 - 1 569216 TGACTAATCAAACTAACCTCAAAACAAATGATAACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCAC >>:>@CC>CAACB@CCA?EDDEHHHC@AEC=7=.>DC:@F=FB??BIHGHGDD?@BD?HGDC:)C?C@CHC<HFAA@F>GDEBD@<AEABFABDBDDD?:? 0 50:G>A,67:G>A,91:C>A -AMELIA:165:C03TUABXX:5:1101:14233:14535 + 1 569226 AACTAACCTCAAAACAAATGATAACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIIJJJJJJJJJJJJJJJJIHHHHEHFFFFFFFEEEEEFEEDDDDDDDDDDDDDDDD>C 0 23:G>A,40:G>A -AMELIA:165:C03TUABXX:5:1101:1740:19198 + 1 569228 CTAACCTCAAAACAAATGATAACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCT CCCFFFFFHHHHGJJJFIJJJJIIJGIIJJJJJIIGIJJIIIJIIIJIIJIHIIJGIJIIIIHG?EHHHFFFFFFFEEFCEBEDDDDDDDDDDDDDDDDDD 0 21:G>A,38:G>A -AMELIA:165:C03TUABXX:5:1101:15763:28944 + 1 569233 CTCAAAACAAATGATAACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGAC CCCFFFFFHHHGHGGIHGHIGIJJJIIGGHIIJGF?BDHFGHICGGGHGHDGEGGIGGH@=EHIHFFHFHHDBDEEDCCCCCCEBBBCDDDBCD?9?@BBB 0 16:G>A,33:G>A -AMELIA:165:C03TUABXX:5:1101:17959:46638 + 1 569233 CTCAAAACAAATGATAACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGAC @@@?DDBD?H?C?AFFHGEIGII>G<;EEFEC@CDHADGGHBGEH@AFEGHHDCHECHG@EHGIEHHHHFHEBDE@;@CCCCC@??@C>CCBCCBCCB95< 0 16:G>A,33:G>A -AMELIA:165:C03TUABXX:5:1101:8672:48013 - 1 569233 CTCAAAACAAATGATAACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGAC BBBCCCADEDDECCCCCCEDACB@ABDA?ECEEEHEHHEC=IIIIIIIIHEEHGFGGGHGHBGGHEIIIIIIIIIIIIIIIIIIGFGBFFHD:D@:DD@@@ 0 67:G>A,84:G>A -AMELIA:165:C03TUABXX:5:1101:20157:48073 + 1 569233 CTCAAAACAAATGATAACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGAC C@CFFFFFHGHHGJJJGIIEGCDHGIJJJJGIIJJGJIHIJDGGIGIGICFHCGHCCHICGHGGHHFEFAHCFFEDBCCCEEEEBDBCDA>BCC??C=<@B 0 16:G>A,33:G>A -AMELIA:165:C03TUABXX:5:1101:1821:61334 - 1 569233 CTCAAAACAAATGATAACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGAC BBDDDDDEEEEEDDDCCDEEFCBCB@>C?FFFFBHEEJHEBJJJIIJJJJJJJJHJJJJIHIJJJJJJJJJJJJJJJJJJJIHJIHIHFDHHFFFFFFBBB 0 67:G>A,84:G>A -AMELIA:165:C03TUABXX:5:1101:2920:60229 - 1 569239 ACAAATGATAACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGC EEEEEEDDDCCDEFEDDDDDDCCDCEEDDFEFHHCHHIJIIJJJJIIJIJJJJJJJJJJJJJJJJJJJJJJJJIJIHJIHHGHEIHGJHFHHHFFFFFCCC 0 73:G>A,90:G>A -AMELIA:165:C03TUABXX:5:1101:1886:48633 + 1 569245 GATAACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACT @<@FFFDDBHFHHGDBBHII?CBB@FEEDFBFCG<FFE@A>DHGH>B./8BCHIG=;=8=@=D@6D7AACEH@?1.766.;>;=;?BBBBCCCCCBCCA<4 0 4:G>A,21:G>A -AMELIA:165:C03TUABXX:5:1101:7720:34723 + 1 569246 ATAACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTC CCCFFFFFHHHHHJJJJJJJJJJJJJ<GIJJJHIJIIJJIJJJIJJGIIJJJJJJJJJJJJJJJJJIHHHHHFFFFFEEEEDDDDDDDDDDDDDDDDDDDD 0 3:G>A,20:G>A -AMELIA:165:C03TUABXX:5:1101:14077:33086 + 1 569249 ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATT @@CDBEFFFHDHGIJIJJIIIIGGI@GAGFHCFFGDHGEIIDH?9DGGHCGCHGIIHDFFIIIJIJGHEEHDEFFEEEDECDB;@?C@@>AB:@A<ACDC: 0 0:G>A,17:G>A -AMELIA:165:C03TUABXX:5:1101:13529:12922 - 1 569250 CCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTT DEEECDDDFDDCDDDDDDDDDDCCEEEEEFFFFFFFGHHHHHJJHJJJJJJJJJJJJJJJJJJIHGJJIJIHFJHFJIHJIHHJIJJJHHHHHEFFFFCCC 0 84:G>A -AMELIA:165:C03TUABXX:5:1101:16196:45964 + 1 569251 CATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTA <<??D;DD?CD?C@DED@FDE8@E)C@D@DADA?BDEB9?4*9DD8?D9CB.B8=BDEDDCA4ADEDDDC@?AD:>>@9=?;325:A>:?:::>A###### 0 15:G>A -AMELIA:165:C03TUABXX:5:1101:17411:56657 - 1 569252 ATACACAACACTAAAGGACGAACCTAATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTAC CDCDDDDDCCDDDDDDDDDDDCDECCCCEFFFFFECHHHHGHIJJIIJJIJJJJJJJIJJJIIHJIIIIHDIHFIHFJIIHIHJJIJJHHHFHFFFFFCB@ 0 75:G>A,86:G>A -AMELIA:165:C03TUABXX:5:1101:6450:57362 - 1 569252 ATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTAT @DCBDDEEDDDCDDDDDDDDCCDEEDEDEFFFFFFDHHHHHHIJJJIJIIJJJIIJHJJJIJIHJIIIGHGIGDJHGJJIIIEJJIJJHHFHHFFFFFCB< 0 0:C>T,86:G>A -AMELIA:165:C03TUABXX:5:1101:6444:57381 - 1 569252 ATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTAC DEDDDDEDDDDDDDDDDDDDC@DEEEEDEFFFFFFFHHHHGHJJJJJJJJJJJJJJIJJJIJIHJIHHHHFIHFJIHJJIJJIJJJJJHHHHHFFFFF@B@ 0 86:G>A -AMELIA:165:C03TUABXX:5:1101:16679:22192 - 1 569254 ACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGGCTCCTGCCTCACTCATTTACAC 3832>>CA::4>??<8@:5(@@3;>CA;>;>;;7DDCBC>HCHHC=;DEAIIHCGHHGAB;9B@B8?B0F?0IHE??<+G7HBEEGDE:=FBBADDBA@?? 0 22:A>G,88:G>A -AMELIA:165:C03TUABXX:5:1101:12972:42420 - 1 569254 ACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACAC DDDDEDC>DDDDDDB?BCCADDEECCDECFDEFDFHHHECHIIJJIGGIGJJJJJIJIJJGIJHHDBD?HD@GG<IHGGHEIIIHIIJHHHHGFFFFFCC@ 0 88:G>A -AMELIA:165:C03TUABXX:5:1101:11063:59818 - 1 569254 ACACAACACTAAAGGGCGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACAC ##############################FFFEFHHHHHFJJJJJJIJJJJJJJJJJIJIIJJIJGIGJHFJIIJJIHJIJJJJJJJHHHHHFFFFF@BC 0 85:A>G,88:G>A -AMELIA:165:C03TUABXX:5:1101:4508:16726 + 1 569255 CACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACC CCCFFFFFHHHHHJJJJJJJJJJJIJJIJJIJIJJJJHIIJJJIJJJJJJJJJJJJJGIIIIGIJIHHHHHFFFFDDDDDDDDDDDDDDDDDDDEEEEDDD 0 11:G>A -AMELIA:165:C03TUABXX:5:1101:7460:21230 + 1 569257 CAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAA CCCFFFFFHHHHHJJJJJJJIJJJJJJJJIJJJJJDIIJJJJJJJJJJJJJJJIJJJJJJJJJJJJJHHHHHFFFDDDDDDDDDDDDDDDDDEFEEDDDDD 0 9:G>A -AMELIA:165:C03TUABXX:5:1101:5559:24757 + 1 569257 CAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAA @<?BDBADFH??BGDHIBICF??CEDH<FF>CEH>??4BFF>9BBFCHCFGCHCC4)=@F;FH:CGGH;AEA>B88<;A>>>@B@ACACA>55>5,4:A## 0 9:G>A -AMELIA:165:C03TUABXX:5:1101:9154:8130 - 1 569258 AACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAAC DDDCDDDDDDDDDDCCDDDDDDDCECEEEEEEFFFFEHHHHHHHJIJJJJJJIIIIGGJIHGCHFGF<HGFJHHHJHIJJJIJIHFJJHHHHHDDDFFCB@ 0 92:G>A -AMELIA:165:C03TUABXX:5:1101:13932:22985 + 1 569260 CACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCA CCCFFFFFHFHHFIJJII;EFHIIIIJJJJJIFHGIJJIIJGHGIJJJJJJJHJJIJJJJJGIJJJJHHGFDFDDCEEDDDDDDDDDDDEEEEDDDDDDD< 0 6:G>A -AMELIA:165:C03TUABXX:5:1101:5470:32337 + 1 569260 CACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCA CCCFFFFFHHHHGJJJJIGIIGHIJIJJJGIJCFIIIJIJIIIIJIJJJIJJJIJIIJJJJJJJJIIHEHGFEDDCEEDDDDDDDCDDDEFEDDDCDDDDB 0 6:G>A -AMELIA:165:C03TUABXX:5:1101:8680:58821 + 1 569268 GACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTA @@@DDDD?AD>DFHEHGGGCHHIIHEGHGGG<BBFAGGGGIDHIGEDDF9BFHGGFIGEGGIEH@G:>EFCCD>D?ACEAADDDECC>BB??BB?B88@AC 0 -AMELIA:165:C03TUABXX:5:1101:11708:29635 - 1 569270 CGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACATCAACCACCCAACTATC DDC@DDDDDDDDDEDEDCDDDDDDDDDDEEDDDEEEEEEFFFFFHHGHGHED;IG@HFDJHGDJHJIIJJIIHFIGJIIJIHHE3IHFHHDHHFEEFFBBB 0 16:C>T -AMELIA:165:C03TUABXX:5:1101:8304:57011 + 1 569270 CGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATC CCCFFFFFHHHHHJJJJJJJJJHJJJJJJJJJJIIJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJIIHHGHHFFFFFFFEEEEEDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:21380:8141 + 1 569275 NTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTTCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAA #1144=2:ABFDC4<<C,,CCECIHIDCCHHEIDAEI@CEAC@BD>F?0?BF?GGDE007)8==F.=CEDE7AA7=AA=?@?=@81;?@?@B((;>>;@BB 0 0:C>N,60:C>T -AMELIA:165:C03TUABXX:5:1101:1970:22449 + 1 569275 CTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAA CCCFFFFFHHHGGJJJIEGHHIJJJJJHCHJIJJIGIJJJJJJGJIIIIJIIHIJJIFHJJJEIIJJGIGGHHHHEHHFF>DEDDDDDDB9?CDDDDDCCC 0 -AMELIA:165:C03TUABXX:5:1101:19426:39932 + 1 569275 CTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAA CCCFFFFFHHHHHJJJJHIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHHHFFFFFDDDDDDDDDDDDDDFEED 0 -AMELIA:165:C03TUABXX:5:1101:18555:51271 - 1 569276 TGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAA :CCCCCCBBC>C>CCCCCACCCEDCA@?CCA>>?CC?CCC>@DDB@;HA6BF<FCFCHDHF=GIGGGGC<GGH@HHGGDCGFGC:GEE>?FFFB?DDD@@? 0 -AMELIA:165:C03TUABXX:5:1101:7071:22548 - 1 569277 GATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAAC CDDDDDEEEDCDDDDDDDDDDEEDDDDDDDDDDDDDDDCEDCECDHHHGJIHJJIHJIJJJJJJJIHIGJIGIHGHIJJHFHHAJJIHHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:3803:28709 - 1 569277 GATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAAC DDDDDDEEEECDDDDDDDDDDEEDDDDDDDDDDDDDDDEEFDFCDAHE@IHFJJJHIIJJIJJIGHFIGJJIIGGHFJIHFHFCJJJHHHHHHFFFFFCBB 0 -AMELIA:165:C03TUABXX:5:1101:5496:29847 - 1 569281 TCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAG BDDEDDADCC@4CADDDEFDDDDDDDDCCCCABBACCCECB?ECAEGGJIIGIGHEIIIGAB?HAIHDIGHF?IGHFGCAIIIHFJIJHHHFFDDBFF@C@ 0 -AMELIA:165:C03TUABXX:5:1101:12408:57891 - 1 569281 TCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAG CDEEEDCDDDDDDDDDDEEDDDDDDDDCDDDDDDEEEAEEB;FHHHJGJIIHJJJJJIJIGGDJIIJIIHGFDJIHGHGCJJJIIJJJHHHHHFDBFFCCB 0 -AMELIA:165:C03TUABXX:5:1101:15680:29851 - 1 569282 CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGC DDDDCCDDDDDDDDDDEEDDDDDDDDDDDDDDDCEECEC@5FEEHIGJJIJJIJJJJJJJIGIJJJIIHGHDJJIHIGFJJIIIIIIHHFHHHEDFFFCCB 0 -AMELIA:165:C03TUABXX:5:1101:5666:55974 + 1 569282 CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGC @CCFFFFFHHHHHJIJJJJJIJJJJJJIJJIJJIJJJJIJIJJJIJJJJIHIJIJJJJJJJIJJJJJJJIGEFHHHFFFDDDBDDDDDDDEEDCEDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:14845:61550 - 1 569286 TACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATG DCDDDDDDDDDDEEDDDDDDDDDDDDDDDCDCCDCA;DCBFHHJIHEIHJJIJJJIGFJJIGCIHGGBJJHGHFAJJJIIJJJJJJJIHHFHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:5581:51725 - 1 569294 TCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCC DDDDEEDDDDDDDDDDDDDDDCDC@AC?8DDBDDDFFFEHHHJJJJJGHFJJJJJJHFFBJIIHHD?JJJIHJJIHJJJIIHHJIJJJHHHHHFFFFFCB? 0 -AMELIA:165:C03TUABXX:5:1101:4720:54536 + 1 569295 CCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC @CCFFFFEHHHHFIIGIJJIGIDGIIJJJJIJJGJIIGFFHIGHIJIHIIIIIJJJGJJGEGHIJGHHGEDDCEDCCEEEDDCDDCDDDDC>CDDDCCBBD 0 -AMELIA:165:C03TUABXX:5:1101:6838:35793 - 1 569302 CATTTTTATTGCCACACCTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAG @?>44>CA:?888C@4(2(++;?=38'?@C>;(<B=?673?HGHE@==CCGF<FDHFFFFB<HGD4GF:1??C?1BC<;HBIHHEEIGD<:;?BDA?D@@@ 0 84:A>C -AMELIA:165:C03TUABXX:5:1101:1542:61054 - 1 569303 ATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGC DDDDDCCADDBDCDCABC?8DB?DBBDDCCCADFDDHHEEAJIJJJIHFC;JJIHIGBIIIHGGGIGHGHFIGEJIIJJJIJIJJJJIHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:7157:20639 - 1 569304 TTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCG >@C>@CCCB@?>@CCA>2+9?;B?8;CC?@=CCBBECAC=E?E@CG@6;5HF;7F@6IGD?:GGDBDGGHDC?IGEHFGCCBEGE?8EHHFC??D:DD@@@ 0 -AMELIA:165:C03TUABXX:5:1101:14737:60270 + 1 569304 TTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCG @@<DDADD<DFFDAFDEECF9CDGFEEEF6CGGA*?B@FGDFB?DFCF@@CCFIIIBF@E@AC;>@?B>>BDBC;>@A;?B:@BBBAA?@@?9?ABBBBA< 0 -AMELIA:165:C03TUABXX:5:1101:17417:5694 - 1 569306 TTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGG DDDDDDDDDDDDCDCA<DB@DDBDCACDBDDDDEEFFDFFFHHHHEE@IHF@HF?JJJIIIGHGIIIGJHGJIIJJJJJIJJJJIIJJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:5461:11293 + 1 569307 TTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGC @@@DFFFDHFHFHIIJIIIIIEIHIHIIJJBHIGFFDHGIJJEGIIGHIGFHGEFGHG;DH>AEEEDDFFCBACCCCCDDDBDDDDD??CBDCCD>ABDDD 0 -AMELIA:165:C03TUABXX:5:1101:18353:12210 + 1 569313 CCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTG CCCFFFFFHHHHHJJJJJJGIJJJJIJJJJIJJJJJJJIJIJIIJJJJJIJJJJJJJJJJHHHHHHFFFFFEEEECEDDDDDDDDDDDDDDDDDDDDDDBD 0 -AMELIA:165:C03TUABXX:5:1101:14671:41117 + 1 569313 CCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTG C@@FFFFFHHHHHIIIHEGGG@EEF>FHGIDHICBFEFHHGGFDEIIBIIIIIG;;D;AEH@EEHHC;@@D>CEC>AC?C?ABC?CDCCCC=B7>955<?C 0 -AMELIA:165:C03TUABXX:5:1101:13866:50046 + 1 569313 CCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTG CCCFFFFFHHHHHJJJJJJJJJJJJJJJIJIJJJJJJJJJIJJJJJJJJJJJCHIJJJJJHHGHHHFFFFFEEEECEDDDDDDDDDDDDDDDDDDDDDDBC 0 -AMELIA:165:C03TUABXX:5:1101:6519:58057 + 1 569313 CCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTG CCCFFFFFHHHHHJJJIIDGGJIGGGGHGJJIIJJJIJJJJJJJEIIFIJJICCHHIJJJHHHGH>BDDFFCEEEEEDDDDDDDDDDCACCBBDDDDDD@C 0 -AMELIA:165:C03TUABXX:5:1101:8012:21023 + 1 569316 CAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATT CCCFFFFFHHHHHJJJHIJJJJJJJGIIIIJJJJJJJJJJJIJIJJJJGBHIIJJJJJJJJJHEEEHFFFFFEEECEDDDDDDDDDCDDBDDDDDD?CADD 0 -AMELIA:165:C03TUABXX:5:1101:17986:22227 + 1 569319 CTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA CCCFFFFFHHHGGFHIIJJJJIGIIJJIIJJJIJJIIGIJIIIIJGGIIJJIJJJIJJHJJJFHHHFFFFFFEEEDDCBDDACCCBDBDDDDDBCCDEEDE 0 -AMELIA:165:C03TUABXX:5:1101:19700:28147 + 1 569319 CTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA @@@FFFFFGDHHHIJJIJJJIIIIJJJJIJJIJJIIIGGIIJIJIGIIJJIJIIJJJJIJJH>CEFFEFFFFDEEDDDDDDDDDDDDDDDDBB8A>>DEDE 0 -AMELIA:165:C03TUABXX:5:1101:11623:32032 + 1 569319 CTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA CCCFFFFFHHGHHJJJJJJJJJIJJJJIJJJJJJJJJJJJJJJJJJGJJJIJJJJJIJIJIJHHHHHFFFFFEEECDDDDDDDDCDDDDDDDDBCDDEEDE 0 -AMELIA:165:C03TUABXX:5:1101:4242:46098 + 1 569319 CTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCATCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA @@<?DDDDHAHA<:CFGCC??CGFGGFGE?BFCF*B@GGH@F*??F8B>C=B8=C=CA;@C)7=@EHE=EEE3@>2;>AC>3(;;;=8<559@?CC:(>@> 0 42:C>T -AMELIA:165:C03TUABXX:5:1101:6403:7733 + 1 569323 CCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJHHHHFFFFFDEEEEDDDDDDDDDDDDBCDEEEDEDDDD 0 -AMELIA:165:C03TUABXX:5:1101:13623:16664 + 1 569323 CCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCT @@@DDFDDDDFFHEH>EGGGICGHEHGIEHEGHIIIIIIIG=DGGIIIICGGEHIIAGGHE@GAHFGFF7???CCACAC@C6?2<B@BBB@>CDDCECCCC 0 -AMELIA:165:C03TUABXX:5:1101:8392:32448 + 1 569327 CTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCG ?@?DDDDDFHDHHEIIEIGIBHGIIIJIIIIJD@FHGG@GHIJEGGIIIJIJCH@GICHGIGIGEFHFFDBCB>CECCBD=<B@BBDCDEEDCC>@CCDDB 0 -AMELIA:165:C03TUABXX:5:1101:4717:30101 + 1 569329 CGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCT CCCFFFFFHHHHHJJJIJJJJJJIIJIJIJJCHJJJ=GHIJIJIJJJJIJJJIIJIJJJJJJJJJJHHHHFFFFFBDDDDDDDBDDEEEDEDDDDDDDDDB 0 -AMELIA:165:C03TUABXX:5:1101:11056:26287 + 1 569335 CCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGA @@<D?DDDFHFFFGHEAF9ECFGHGEGEDEEEH<FAFG@EGHIGIEIGFHGG>FCGGHG@CACHIIIFHB<A>BBB@?>@DDDC>ACCACCCBBBBBCAC: 0 -AMELIA:165:C03TUABXX:5:1101:1371:45743 + 1 569335 CCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGA CCCFFFFFHHHGFIJJJIJJJJJJJGGHJIBHIIIGIIJJJJIJIGIIIHIJFIIIJJJIJJJIJCIFFGFDDDDDDDDDEDEDDCCDDCDDDDDDDDDDA 0 -AMELIA:165:C03TUABXX:5:1101:12014:36248 + 1 569336 CTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGAT @@@DDDBDAFHHHDIGIGBFCCFH@FCF?:EGCF@C@FGH@G@BF@FB?<98B=;=BFGHG@CAE77?EDCB=BBB2>C@CCCCACCA?CA@B5?B##### 0 -AMELIA:165:C03TUABXX:5:1101:2823:24901 + 1 569340 CTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAA CCCFFFFFHHHHHJJJJJJJJJJJJCHIJJIJJJJJJJGIJJJJIIJJJJIJJIJJIJJJJJJIJHFDDDDD@DDDEEDEDDDDDDDBDBDDDDDDDDCDD 0 -AMELIA:165:C03TUABXX:5:1101:4911:36065 + 1 569340 CTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAA CCCFFFFFHHHHHJJJJJJJJJJJJ=GHIJJJJJJJJJJIJJJJJJIJJJJJJJIJJJIJJJIIIHFDDDDD@CDEEEDEDDDDDDDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:14894:13781 - 1 569341 TCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAA CCCDDEEEEDBBBDDD@DB?DDCB@CEDCDDDDDC>EECFFFFFHHHHJHIJIJJIJIHJIJJJJJJJJJJIJJJJJJJJJJJJJJJJHGHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:13273:24919 + 1 569344 CTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATG @@@DDBDDHHHHDGHG@FHAC+AGEHDD>FHIIIIIBG>DFHIFIIGGHGIIDICHGGHIIE>BBBBB<>AB>5@@C@C>CCC85<BBC@C@CCCCCCCBC 0 -AMELIA:165:C03TUABXX:5:1101:7279:56381 + 1 569344 CTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATG CCCFFFFFHHGFHJIJJJJJJEHIJJJJJIJJJJJJJIJJJJJIIJGIGHIJJJJJJJJIJIHDDDDDDCCFEDDCCCCDDDDDDDDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:2681:11009 - 1 569346 CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCC DEEECABBDDDBD@8DDDDCDDDCDDDCDC>DDECEEEFFFFFGGGJJJJJJJHIIGGIJIIJJJJIIJIGIGGGFIIGJJIJIJJJJHHHGHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:3875:13885 - 1 569346 CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCC EEEEDBDDDDB>D>8DDDFDDEEDDEDDDC>DDEEEEEFFFFFHHFJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:3645:57765 - 1 569346 CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCC >B>EDDB@DDBBDB?DDDFCDEEDDEDCDC@DDEEEEEFFFFFHHEIJJJJHJIJJJJJJJJJJJJGJJJJJIJJJJJJJJJJJJJJJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:6450:16039 + 1 569347 ATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCC CCCFFFFFFHGHGIIIIE@FGIIIIIIIIIIHIIGHGIGGGIIIIGIIHIIIIGEHFHGHDDDBD?@CDDEDECCCDDDDDDDDDDCDDDCDDCDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:15885:23137 - 1 569350 TACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAG CAB?DDB<B><DDDDCDEEDDDCCCACDDDCBDDEEEEDFFDHHHIJJJIIJIJJJJIIJJJJJJJJJJJJJJJJJJJIJJJJJJJJJHHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:1931:36381 + 1 569352 CACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCC @@@ADFDDFDF@FEGGIDH@HCGHIIDIH?<DBDFEGGGAGCBHEFG>AFBD<FGHBCBB;@ABDDCEAAC:<A>?9<B?CCCCCCCCCCC?CC>ACCCCC 0 -AMELIA:165:C03TUABXX:5:1101:14804:56235 - 1 569352 CACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCC ??DB@9DB@DDDCCDEEDDDDDCC:BDDDDDDDDEEDDDDFHHHHHJJJJJJJJJJJJIJIJJJJJJJJJJJJJJJJJJJJJJJJJJIHHHHGFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:6540:18322 + 1 569355 CAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCAC CCCFFFFFHHGHHGEHHJJIJIJJIJGIJJJIGHIIJJJJJJIEIJIJJIIIIFDDDBACDEDDEDDDDDDDDDDBDDDDDDDDDDDDDDDDDDDDDCCBD 0 -AMELIA:165:C03TUABXX:5:1101:17325:18593 + 1 569355 CAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCAC CCCFFFFFHHGHHJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJFDDDBDDFEEDEDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:10156:26124 + 1 569355 CAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCAC @@@BD:DDHH8FFFHGBEDHHHHIDF<F?<?C?;DH>DG??FHIID<8?FBBG>A63,;35>@ACCCC@9?>?@B8?CCCCCCCCCCC@CC>C>(<@C9<< 0 -AMELIA:165:C03TUABXX:5:1101:11937:34471 + 1 569355 CAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCAC CCCFFFFFHHGHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJFDDDBDDFEEDEDDDDDDDDDDBDDDDDDDDDDDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:6308:58365 + 1 569355 CAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCAC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJFDDDBDDEEEDEDDDDDDDDDDDDDDDDDDDDDDDDCDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:5603:62616 + 1 569355 CAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCAC @@@DDDDD?FAFHDGHBF,AFEDG9E9CFH>G9DFHCDGHCGIE?FD<?FGH@9/3,,5A:@>C>C>::5>:@B588>C:44@ACCCA@CCCC4<9>?>?< 0 -AMELIA:165:C03TUABXX:5:1101:16475:16825 - 1 569362 CCAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTAC DDDCDEEDDEDDDC@DDDDDDDDCCCDDDDDEDEECFDFHHJJJJJJIIJJJJJJIIIHIGJJIJJJJIG<JJJIIJJJJIIIJJJJJHHHHHFFFFFC@@ 0 -AMELIA:165:C03TUABXX:5:1101:12218:53027 + 1 569363 CAACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJIJJHHDFFFFFFEEEEEEDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:8175:22480 - 1 569365 ACTATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCAC CCDCCDDCCDDDDDDDDDDDDDDDDDDDDDEDECBFFHGJGIGIJIIIIJJJJIJJIJJIGJIJIGGJJIJJIJJJIIIIJIIIJIGHFBHAFFFDDD@=@ 0 -AMELIA:165:C03TUABXX:5:1101:8017:3498 - 1 569368 ATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAG EDDEDDDDADDDDDDDDDDDDDDDDDDDDDDDDDFFHHIJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJIHFJHHHHHDFFDFCCB 0 -AMELIA:165:C03TUABXX:5:1101:7419:29811 - 1 569375 AACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACC CCDDDDDDDDDDDDDDDDDDDDDDBBDDDFFFHHGJJJJJJJJJJJIJJJIJIJJJJJJIJJJJJJJJJJJJJIJJJJIHJJJJJHGIHHHHHFFFDDBCB 0 -AMELIA:165:C03TUABXX:5:1101:3979:55069 + 1 569378 CTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTAC ?@@BB?;DCADDABEDHBHDADBHIEFBA>ABEIEEGIGGC@CCEHFHEEED@B@CCCCCCCCCCBCC@C:9?CCCBBB@C@>CC?CCBBBBBBACBBCCC 0 -AMELIA:165:C03TUABXX:5:1101:16700:24570 - 1 569383 CATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCC CCCCCBBBBBBCCCCCBBBBBBBBBCCCCCEEEEEEFEAEIGFIIIIIIIIIIIIIIIIIGIIIHGIIGIHGEIEHEHGGF<IGHHEHDFCHHDD@=D@@@ 0 -AMELIA:165:C03TUABXX:5:1101:15721:53058 + 1 569385 TGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTT CCCFFFFFGDHHFIJJGJJFGDEEHBHFHIJJIGIIJJJJJEIHGHFFFFFFFECEEEEDDDDDDDDACBDDDDDCDDA@CBDBDDDDDDDDDDCDABDDD 0 -AMELIA:165:C03TUABXX:5:1101:18071:29044 + 1 569387 GCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTAT @@CFFFFFHGHHGJJJJJJJJJIJJFIJJJJJJJJJJJJJJJFHHHHFFFFFFEEEEEEDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDC 0 -AMELIA:165:C03TUABXX:5:1101:3960:44523 + 1 569387 GCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTAT CC@FFFFFHHGHHJGIIIIJJJIJJFHIGIIIIJIIIIJJCGFGHHHFFFFEFEEEECDDDDDDCCCDDDDDDDDDDDDDDD@BBDDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:4173:30083 - 1 569388 CCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATC BDDBDDDDCDDDDDDDDDDDDDDDCDEEEEEEBDFFHFJIJIEGJJJIIJJJJJJIJJIIGIHHEF?JJJIGHFIGEJJIIIGIHEGIHHFCHFFFFDB@B 0 -AMELIA:165:C03TUABXX:5:1101:11884:56290 - 1 569389 CATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCC DBDDDDDCDDBDDDDDDDDDDDDDDDDEEEEEDFFHHHJIJJJJJJJJJJJJJJJJJIGJIJJHFFJJJJJJIJIIJJJJJHHHFJIHFFCHHFFFFFBCB 0 -AMELIA:165:C03TUABXX:5:1101:1856:6021 + 1 569390 ATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCC CCCFFFFFHHHHHJJJJJJJJJEHIJJJJJJJJJJJJIJJJJHHHHHHFFFFFFEEEEEDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:2846:21330 + 1 569390 ATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCATAAGGCACACCTACACCCCTTATCCC CCCFFFFFHHHHHJJJJJJJJJHIJJJJJJIJJJJJJJJJJJHHHHHHFFFFFFEEEEEDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 0 75:C>T -AMELIA:165:C03TUABXX:5:1101:4769:42629 + 1 569390 ATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCC @@@FFFFFBHHAD>DGGHHIEBFGIIIIIIHHGEHGIIIIIIHGFFHFFFFFFD>>@CECCCCC@?BCCCCCCCCCBBB?A?BCBBCCCCCCBBBBCCCCC 0 -AMELIA:165:C03TUABXX:5:1101:10476:53401 + 1 569392 CCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCA CCCFFFFFGHHHHJJJGIJIHHGIIIGDHEGJIJIFGIIJIIGHHHHHHFFFEFFDEEEEDDDDBDDDDDDDDDDBADDDDDDDDDDDDDBDDDDDDDDD< 0 99:T>C -AMELIA:165:C03TUABXX:5:1101:13726:24695 + 1 569393 CCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCAT @@CDDDFAHHBHFGGEEGGGDHIIIEDFCDHHIGCGEHIEEAHGHEEHHHFFDCFEECCEDABACCDD@CDDC?AA??BBDD@DCCD?CDDDBDCDCDDC? 0 98:T>C -AMELIA:165:C03TUABXX:5:1101:4165:12349 + 1 569395 CTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATAC @@@FFFDDHGHDFIGHGIIIJJGIIJIJIJJDDHGDFGEHHIIGGHGHHBFFFFE@@CCBD?CDDDCDCCDBDDDDDDDDDDCDDDDDDDDDDDDCDDCD? 0 96:T>C -AMELIA:165:C03TUABXX:5:1101:8346:53208 + 1 569405 GGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTAT @@@FFFFA?DDFFHIDDEBGFG@EFGDEGGGHIIIIIIIIIIIGHGGHGIIGGIIIIIIII@DEADEHHHDC?@B@CAB@ACCCCCCCACCCCC5@CDCCA 0 86:T>C -AMELIA:165:C03TUABXX:5:1101:18458:3921 - 1 569407 CGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCG DDDDDDDEDDDDDDDDDDDDDDEEEEEEEFFFFFHHGHHJJJJIJIIHJJJJJHFIHDJJJJHHGHFIHGDD:JJJJJJJIJJJJIIHHHHHHFFFFFCCC 0 16:T>C -AMELIA:165:C03TUABXX:5:1101:10235:44701 - 1 569407 CGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCG DDDDDDDEDDDDDDDDDDDDDDEEEEEEEFFFFFHHHHHJJJJJJIJIJJJIIHFIHDJJJIIHFGFIIGDD?JJJJJJJIJJJJJIHHHHHHFFFFFCCC 0 16:T>C -AMELIA:165:C03TUABXX:5:1101:11767:4386 - 1 569410 AGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAA @CCCECCC@CBCB@BBB>A;5;>;C@C;>??C;=ACHCEAED;@)C=8=CDGHEHHDIGGBB90B?:0?)G?A9<??BG;CC<FIHF?CCCFA?BDAD@@@ 0 19:T>C -AMELIA:165:C03TUABXX:5:1101:9456:30886 - 1 569411 GTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAAC CCDEDDDDDDDDDDDDDDDDEDDDDDDDEEFFECFEHHHIGHA@JJIIGHDIHFJJIHFDBD?GGFDD?JIJJJJIGGIIHHHEGIJJHHHHHFFFFF@@@ 0 20:T>C -AMELIA:165:C03TUABXX:5:1101:19751:8207 + 1 569418 TAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGC @CCFFFFFHHHHHJJIJIJJJJJJJJJJJJIJJJJJIJJJJJJIJJJJJJJJJJJJJJJJJJJIJHHHFFFFFDECEEEDDDEEEDDEDDDDDDDDDDDDD 0 73:T>C -AMELIA:165:C03TUABXX:5:1101:16276:24262 + 1 569418 TAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGC CCCFFFFFHHHHHJJIJIJJJJJJJJ<FHIJJIJJJJJJJIJJJJJJJJJJJJJJJIJJJJJJJJHHHFFFFFDEEEEEDDDEEEDEFDDACDDDDDDDDD 0 73:T>C -AMELIA:165:C03TUABXX:5:1101:20445:2941 + 1 569423 TTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACT CCCFFFFDHHHFFIIJGIJIGIJIJJIIIJJ?GGJGIIJJGBDBFHIIJHIJJGGIJJIJJIGIGHHHHFFFFFEEEEDEEFEDD9??B@DCCDDDDDDD> 0 68:T>C -AMELIA:165:C03TUABXX:5:1101:19937:42503 + 1 569423 TTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACT CCCFFFFFHHHHHJJJJJJIJHJJJJJJJJJGIJJJJJJJJJJIJJJJJJJJJJIJJJJJJJJJHHHHHFFFFFEEEEEEEFFDDDDDDDDDDDDDDDDDD 0 68:T>C -AMELIA:165:C03TUABXX:5:1101:9395:20740 - 1 569427 GCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATT CDDDEDDDDCDDEDDDDDDDDBEDBC@@DFEFFEAHECIIIGEEHC@IGHBA?IIIJJHG=IGIGJJIHHJJIIEEGIIJJIHGJIHEGHHHHDDEDFB@@ 0 36:T>C -AMELIA:165:C03TUABXX:5:1101:21021:46440 - 1 569428 CTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTC DDDDDDCDCBCDDDDDDDDDDCDCC>;C>>C>@AE?=HIIHED;F@HC@6F?JJJIHEHCJJIIHGFGIJHIGGEGGFIJIHAJJJGJHHHHHDFFFF@@B 0 37:T>C -AMELIA:165:C03TUABXX:5:1101:16159:12925 + 1 569430 CTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA CCCFFFFFHHHHHJJJJJIJIIJJIJJJJJJJJJJIJJJIJJJJJJJIJJJJJJGIJJIJJJIIJHHHHEHFFFFEFFDDDDDDDDDDDDDDDDDDDEEED 0 61:T>C -AMELIA:165:C03TUABXX:5:1101:13920:43623 - 1 569431 TAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAAC EDDDDDDDEDDDDDDDDDDDDEECEEFFFFDFHHHHHJIHHHFJHHFF?JJJJJIGGJJJJIHBJJJJJJJJIIIJJJIHJJJIJJJJHHHHHFFFFFCBB 0 40:T>C -AMELIA:165:C03TUABXX:5:1101:16101:61244 - 1 569432 AAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACC DDDDDDDEDDDBDDDDDDDDDEEEEEEFDBFFHHHHHIGCE@IHG@A6JJJJHHHDJJJIIHFJJJJJJJJJJJJJJHHJJJIJJJJIFFHHHFFFFF@CB 0 41:T>C -AMELIA:165:C03TUABXX:5:1101:12658:53677 - 1 569436 TTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATA :BCCDDDDDDBDDDDDDC@DDDCAC>DECDFDEHC=?3IHCAA;IIHF<D@@IIGGGHFGAEIIIGIIIIIIIHFIGHCIIGA?A<GDFFHFHFEFFD@@@ 0 45:T>C -AMELIA:165:C03TUABXX:5:1101:19748:57145 - 1 569436 TTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATA :A@DAC?<?A?B???=C@;C>CC@A@?ECFFEFGHE?)GIHBF<HCF>HEIHJJJIIDBIIJIJJJJJIJJJIHHJJJIJIIIHIGJIHGHFHFFFFFCCC 0 45:T>C -AMELIA:165:C03TUABXX:5:1101:7806:60397 - 1 569436 TTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATA DDDEDDDDDDDDDDDDDDDDDDEEEEDFDFFFFHHEE?IIHBF@JJIJJJIIJJJJJIHJJJJJJJJIJIJJJJHJJJIJJJJJIGJJHHHHHFFFFFCCC 0 45:T>C -AMELIA:165:C03TUABXX:5:1101:8649:16269 + 1 569439 AAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCC CCCFFFFFHHHHHJJJJJJJJJJJJIJJJJIIJJJJIJJJJIJJJJJJJJJJJJJJJJGIHIJJIJJHHHHFFFDEEEEEDDDDDDDDEEEEDDDDDDDDD 0 52:T>C -AMELIA:165:C03TUABXX:5:1101:9638:16018 + 1 569440 AAATGCCCTAGCCCACTTCTTACCACAAGGCACACCCACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCC @@@FFFFFGHHFHIDHIIGIJJIJIGIJGJIIIIJG)BFFHJJIHIGGGGGIEHGIIHIIIIDHHHHHFFFDCDDCCCCCDDDDDDDEED>CBDDCDCCA? 0 36:T>C,51:T>C -AMELIA:165:C03TUABXX:5:1101:3989:21801 - 1 569442 ATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTG CDCBBBDDDDDCCC@ACDCDDC@ECCECABB;EDA:=5IIHEIIHFIIJIGD?G@IIGGGIHAEGGFECCGGFGGGHHCAHGIHGGGEFFGHDFFDDD<<@ 0 51:T>C -AMELIA:165:C03TUABXX:5:1101:20449:61208 - 1 569443 TGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGG DDDBDDDBDDDCDDDDDDCBDDDEC@EB>C?FFD?BBJJIIIJJIJJJJJJIIIJJIIIJIIGJHIHGIIHFIJJJIJHIIJIGGIIHHHHHHFFFFF@CC 0 52:T>C -AMELIA:165:C03TUABXX:5:1101:14321:32221 + 1 569451 CCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGC @@@FDDFFHHFHBFHIGEHEHICHGIIIIIGEFHIIIIIIIFGHHEGG9DAHIGGCGHAHHEAHGEHHGEFEDFFACAADEEB;C>@CCCCB?A93)5?<> 0 40:T>C -AMELIA:165:C03TUABXX:5:1101:21136:39819 + 1 569452 CCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCC CCCFFFFFHHHHGJJJJJJJJJJIJJJEIJIIIIIJIJJJJIGIJJJGIIJJJJJJIJJJJIJIGHHHHFFFD>CDEEEEEDDDDDDDDDDBDDBBDDDDD 0 39:T>C -AMELIA:165:C03TUABXX:5:1101:5561:47117 + 1 569452 CCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCC CCCFFFFFHHHHHJJIJIGJJJJJJIJJJIJJJJJJJJJJJJIIIJJFIIJJJJJJIJJJJJJJJHHHHHFFFFFDDEEEEDBDDDDDDDDBDDDDDDDDD 0 39:T>C -AMELIA:165:C03TUABXX:5:1101:17488:56246 + 1 569455 CTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAA CCCFFFFFHHHHHJIIGIIIIIJJGIJJJIJIIJJJJIDIIIJJGIGJJJJJJGHIJJJJJIIJJHGHHHEFFFFEFCDDCDDCDDDDDDDDDDDDDDDDD 0 36:T>C -AMELIA:165:C03TUABXX:5:1101:16689:60778 + 1 569458 CTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCG @@?DBDDBHAH?FGGIHIIIEEFHHH@E?FHGHIEHHIIDCHGGHIGGHDHIGGICDGDHIGHBECEHEFFDDFFBB@CCAC?CB;??B3;?BBBBBABDB 0 33:T>C -AMELIA:165:C03TUABXX:5:1101:8687:22414 + 1 569462 CCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAA CCCFFFFFHHHHHJJJJJJJJJJJJIJJJJJJIJIJJGIIJJJJJJIJIJJIJJJJJJIJJJJIHHHHHHHFFFFEEEEEDDDDD@BDDDDDDDDDDDDDB 0 29:T>C -AMELIA:165:C03TUABXX:5:1101:11178:48721 + 1 569462 CCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAA ???DDDDDFADDFHGHB?E?GHIEGGIIIBHCFHIIGIICHHDEHGCDGGHIEFCFHIGGFGEHHHCEEH>;BDBA@CCCBBB=?;BBBBBBBCBBBB9>> 0 29:T>C -AMELIA:165:C03TUABXX:5:1101:12502:5970 + 1 569463 CACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAAC CC@FFFFFHHHHHJJJIJJJJJJIJJJJJJIJJJJJJIIJJJJJJJJJJJJJJJJJJJJJJIJJJHHHHHHFFFEDEEEEDDDDBDDDDDDDDDDDDDDDD 0 28:T>C -AMELIA:165:C03TUABXX:5:1101:2833:14715 - 1 569463 CACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCGTTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAAC @:BA>AA8+4+:>:5509?BB>?=8?B?@B@;>;>AAAB?;;;1DB;?;>?;;FEE@@@5'6)CF;8?FD@FCFED=?EGGA<E?E?<BB??C6??D?;@@ 0 38:A>G,72:T>C -AMELIA:165:C03TUABXX:5:1101:20500:30078 - 1 569463 CACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAAC CCDDDDDDDDCDDD><5DDDDDCB?BDDEEDADDEEEEEDFFFFEHEAHHIGJJJJIGHJGJJJJJJJJJJJJJJJJJJJIJJJJHJJHHHHHFFFFF@BC 0 72:T>C -AMELIA:165:C03TUABXX:5:1101:4190:51441 - 1 569467 AGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTA DDD@CA@DDC@>>DDDDDDD?DDDDCDCCDDDDDEDDFCFFFDBHHHFFJIIGIHJJJJIJJIHJJJJGJJIJIGJIIIIGHIJIJIGFGHHGFFDFFCCB 0 76:T>C -AMELIA:165:C03TUABXX:5:1101:9899:2962 - 1 569468 GGCCCCCCTACCCCCCTTAACCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTAC ###################################DEDCFDC?HHHEHIIJIIEIIJJJJJJHJJJJIJJJJJIJJJJJJIJJJJIJIHHHHHFFFFFCBC 0 77:T>C,81:T>A,89:A>C,95:A>C,97:A>C -AMELIA:165:C03TUABXX:5:1101:13542:15249 - 1 569470 CACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTG ?@A:CC><<5DDDC@D@?DDC>C@CCDDDDDDBCEC?;BB;FDHCEEHIGHCIEIGIJJIIJJJJJJJJJJIJIGJJJJJGIIGEEFGFHFHFFFFDB@@@ 0 79:T>C -AMELIA:165:C03TUABXX:5:1101:3921:23753 - 1 569470 CACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTG @CCCDDC<>>DDDDDCDBDDDEDDCCDDDDDDDDFDDFFFDFFHAHHHIGHGIIJJJIIJJJJJJJJJJJJJJJJJJIHJJJJJIHJJHGHHHFFFFFCCC 0 79:T>C -AMELIA:165:C03TUABXX:5:1101:20324:42404 - 1 569473 CCCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAG 0A>45<<BBBBA?9BBBBABA>>B@>>;(;BDBBA@@?DDBBDAFFEEEECEFFFFF@DEEFIECFFD?0:FEDC8FBFAD@CGFA22<,C<D;DDDD@@@ 0 82:T>C,100:A>C -AMELIA:165:C03TUABXX:5:1101:14130:52644 - 1 569473 ACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAG :DCC>@<DDDDDDDBDDDEFC@DDDCDEDDDEDDEEC@FFFFFHHHHHGJJJJJJJJIJJJJJJJJJJJJJIJIIHJJIJJIHJJJJJHDHHHFDFFFCCC 0 82:T>C -AMELIA:165:C03TUABXX:5:1101:9169:42274 + 1 569475 CTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGC CCCFFFFFHHHHFIJJJGIHIIJJIHJJIJJJJJJJIGIIGIGIIJIIJJJJIJJIJIJIGHHIIJIGHHHFFDDDDDDDCBDBDDBBCCDDCDDACDCDD 0 16:T>C -AMELIA:165:C03TUABXX:5:1101:15411:8706 + 1 569481 CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTA CCCFFFFFHHHHHJJJJJJJIJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIHHFFFEDEEDDDDDDDDDDDDDDDDDDDDDDDDC 0 10:T>C -AMELIA:165:C03TUABXX:5:1101:8186:41220 - 1 569484 TTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTC @DDBDDDEDD@DDDDDEDDDEDDDB@>DDDDDDEEEECEFFFFFFHHHHHJJJJJJJIJJJJJJIJIJJJJJJJJJJJJJJJIHJJJJHHHHHDFFFDCBB 0 93:T>C -AMELIA:165:C03TUABXX:5:1101:10597:31275 + 1 569489 CCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCA CCCFFFFFHHFHHJJJJJGIIGIIJIJJJJJIIJIJJJJIJIJGGIGIGHIJJIJJGJJJIJJHHGFFFDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 0 2:T>C -AMELIA:165:C03TUABXX:5:1101:4678:13701 + 1 569492 ATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCT CCCFFFFFHHHHGJIJGIIJJJJIJGIIIJJJJIJJJJIJEIJEGCHIIJHGJJJIGGIIJIGHHFFDDDDDDDCCDCCCC?ADDDDDDDDDDDDDDDDDC 0 -AMELIA:165:C03TUABXX:5:1101:16990:2775 - 1 569495 CTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAAT DDDDDEDDDEDDDDCADDDDDDDDCA@DDDDDDDCAEEFFFFFHHHIJIJJJGIJJJJJIHJJIJJJJJJIHEJJJJJJJIHFJIHGIHHHHHDBFFFCCB 0 -AMELIA:165:C03TUABXX:5:1101:6297:45734 + 1 569495 CTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAAT CCCFFFFFHHHHHJJJJIJJJIJJJJJJJJIJJJJJJJJJIIIGJJJJJJJJGHJJJJJJHHHFFDDDDDDDCDDDDDDDDDDDDDCDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:2359:60066 - 1 569495 CTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAAT DDDDDDDDDEDDDBCADDDBDDDDCCADDDDDCDC@EEFFFFFHHHIIJJIJJIJJJIGGIJIJIIIIIIHGFIJJJJJJIGFJIHFIHHHHHDAFFFC@B 0 -AMELIA:165:C03TUABXX:5:1101:18333:14858 - 1 569497 AGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTG CDDEDDDEDDDDC@DDDDDDDDDCACCDCCCBB>EEEEEFFFHHHIJJJIIIJJIIGJJJJJIIJJIIGHFHGIIIIJIHEIIIHJHJHFHHFEFFFF@@B 0 -AMELIA:165:C03TUABXX:5:1101:19873:36037 - 1 569497 AGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTG CDDEDBDDBDDDCCDDDDDDDDCC>DDDDCDDBCEEEEFFFFHHHJIJJHEHJJJJIHFJIGJJIGJJJIHHEJJIJJJHHIHFEIGIGHHHFFFFFFC@@ 0 -AMELIA:165:C03TUABXX:5:1101:15244:48567 - 1 569500 TATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAA EDDDEDDDBC>DDDDDDDDCCADDDCDDDDDEEEEEEFFFHHHJJJJJIJIJJJIHJJIIJJJJJIHEJJJJJJJIHFJIIHJJJJJIHFHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:19720:55417 - 1 569500 TATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAA EDBDEDDDDDCDDDDDDDDDDCDDDCDDDDCEEEEEEFFFHHHJIJJJIJJJJIIGJIIJJJJIIIIHJIJJJJJJHHJIHJIIJJJJHFHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:16495:20629 - 1 569501 ATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAG DDDEDDDDDCDDDDDDDDCC@DDDDDDDDCDEEEEEDFFFHHJJJJJJJJJJIHGJJIJJJIJJJGHIGJJJJJIHFJIHFJJJJIGGFHHHHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:13713:36730 - 1 569501 ATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAG DDDEDDDBC>DDDDDDDDDCCDDDDDDDDDDEEEEEDFFFHHJJHIFGJGJIIHFJJJJJJJJJIHEIIJIIJJIGEIIHEJIJJJIGCHHGHFFFFFCCC 0 -AMELIA:165:C03TUABXX:5:1101:21204:4040 + 1 569502 TTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGC CCCFFFFFGHHHHJIJJJIJJJJJJJJIJJIIIIJIJIJHIJIJJBHIIJJIIGIIHHFFFDEEEECCCDDCDBDADBDDDCDCDCDDDDDDDDACCDDD? 0 -AMELIA:165:C03TUABXX:5:1101:9909:9961 - 1 569502 TTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGC BBDBBCBC:CCC?BCCCCCACCCCA:B?CCCCCB@CEEEEHIIIIHFIIGIGF?IIEHGIGIGEHFHDGCBGHEEEIHF<HGIBGEA<?FHFBDDDDD@@@ 0 -AMELIA:165:C03TUABXX:5:1101:2110:26628 - 1 569504 ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCAC EDDDDCADDDDDDDDDDCDDDDDCBACDDDDEEDDFFFHHJJJJHJJJJJJHJJJJJJJJJIHGJIJJJJJIHFJJJJJJJJIJHGJJHHHHHFFFFFCCC 0 1:G>A -AMELIA:165:C03TUABXX:5:1101:2178:51747 + 1 569504 ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCAC CCCFFFFFHHHHHIJJJJJJJJJJJJJJJJJJJIJJJJJJJIJHJJJJJJJJJJJHHFFFFFEEEEEEEDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDD 0 99:G>A -AMELIA:165:C03TUABXX:5:1101:20752:21440 - 1 569505 TCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCACC BDCBCCDDDBDDDDDDCDDCDDDDBDDDDDDEDDDFFFHJJJJHJJJJJIHJJJJJJJJJJIHJJJJIJJJHHJJIHJIJJIIGEJJJHHHHHFFFFD@CC 0 2:G>A -AMELIA:165:C03TUABXX:5:1101:19065:27787 - 1 569505 TCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCACC BBCBC@CCCBCCCCCCCCCCC@:@?CCCC@BACA??<DE@@AF<HHGBGG@IIIIHIIIHGF@HBDFBIHEGH@D@EGEGIIIIHEEIHFAFHFDDDA:@? 0 2:G>A -AMELIA:165:C03TUABXX:5:1101:16603:56285 - 1 569505 TCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCACC BBCB@:CCC<BC@A>55:CCAAC?;->CCCBBABCB<BAA8A@;A==87..@@B@DD:?D<C?D;?;C?BCAAGH@IGHBA:A@?HGHDDHHDDDDDB@<@ 0 2:G>A -AMELIA:165:C03TUABXX:5:1101:1987:34494 + 1 569512 CATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCACCACCCTAG CCCDBDFDHHHHGIIIIIIIEHIHIIIGFIGGIII?FBDFFHGIIIIIIIGIGIGHHGFGDCFFDCCEDDDDCDDCCDDDDDCDEDDCCCBDCBDDDDDDC 0 91:G>A,96:A>C -AMELIA:165:C03TUABXX:5:1101:14178:4981 + 1 569522 CTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCACCACCCTAGCAATATCAAC CCCFFFFFHHHHGIJEHIJGHIJJJHIIBHHIJIJJEIJEIIJIGIIJJJJIGJHGGFFFFDEECEEEEDDDCDEDDDDDDDDDBDDDDDDDDDDDDEEDD 0 81:G>A,86:A>C -AMELIA:165:C03TUABXX:5:1101:15521:44221 - 1 569522 CTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCACCACCCTAGCAATATCAAC DDDDDDDDDDDDDDDDDDDDDDDDDCCFFFHHHHJJJJJJJJJJIHJJJJJJJJIFJHHDJIJJJIHFJJJJJJJJJJJJHHHGCJJJHHHHHFFFFFCCC 0 14:A>C,19:G>A -AMELIA:165:C03TUABXX:5:1101:16516:5737 - 1 569605 CACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAG ?ADDDDDDEFEDDDBC@DDEDCC@CBEBFHHFHEDHDIIJJJJJIIIHHHJJJJIJIJJIIIIIJJIIJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC 0 82:T>C,97:A>C -AMELIA:165:C03TUABXX:5:1101:13234:12614 - 1 569605 CACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAG ?>BBCCCCEDEECCBA>CFDCCC>BBEEEHEACA@D=EIIGECGEGGCB=>HGB?GGIGGHHEIHFB:@D>GFIIIGHGCAFBAIGBDD?D??DD;?B@?@ 0 82:T>C,97:A>C -AMELIA:165:C03TUABXX:5:1101:5183:14985 - 1 569605 CACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAG 8:BB@CDDEC@CCABDDCDEDEC@BADEFGHEEECGDEE=HGGGGHGFHHIHIGGIIHGCFGFDIIIIGIGIHHEIIGHHHIGGHGEGDFDC?DDFDD<@? 0 82:T>C,97:A>C -AMELIA:165:C03TUABXX:5:1101:5239:31956 - 1 569605 CACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAG ??DDCCDDEEFEDCBA;DDEDEC>A?B>FHGGHHCD@GJHIGJJIIGGHFJJJIIJJIGGGGDDGIIIIEIIIJJJJJJJJJJJJJJJHHGHHFFFFFCCC 0 82:T>C,97:A>C -AMELIA:165:C03TUABXX:5:1101:10507:35056 - 1 569605 CACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAG 8:?ACCACA;;>;9?;5@C>5;;6==?5<CEECBHEE=7.7=@=)=@)==<CADHB<B998B99FBHF?*??HFC??FD@GGIF@GDHDHF???DDAD<@? 0 82:T>C,97:A>C -AMELIA:165:C03TUABXX:5:1101:1362:46510 - 1 569605 CACCCTAGCAATATCAACCATTAGCCTTCCCTCTCCACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAG 83?<55>A>;;;3?@>>(5;;@5(;5>9B?57..)..=.)4FFAC==;8.<F<BFB899*FFDB?0D:D<FGF@?:D@BEA?+G<FC83?F?FADDB=@@@ 0 66:A>C,77:A>G,82:T>C,97:A>C -AMELIA:165:C03TUABXX:5:1101:16406:46639 - 1 569605 CACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAG ?>DDDEDDEEDDDCDC>DDEDCC@C?B@FHHHHEDD=IIGIGIIGJJIHFJIIGDJJJJIIIGGJJIHIIJHJJIJJJJJJJJJIJJJHHHHHFFFFFCCC 0 82:T>C,97:A>C -AMELIA:165:C03TUABXX:5:1101:18526:48170 - 1 569605 CACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAG ?:DBDDDDEFDDC?B@5DDEDC@;CCB<FHHHHA>D@GGIJHHHGJIIHDHCJIDJIIIIGGGDIIGGIIIHJJIJJJJJIJJIGIHIGHHGHFFFFF@CC 0 82:T>C,97:A>C -AMELIA:165:C03TUABXX:5:1101:12228:54596 - 1 569605 CACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCCCAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAG ##@?BA:5(B@>535(;@>A>;;-;?>:DDBEEA=EEECC=7772@DF=)388)*AF<<B<D<??F?B?<B?BEFGF;=E:?8:@CE@:<D:?B;??D?@? 0 51:A>C,82:T>C,97:A>C -AMELIA:165:C03TUABXX:5:1101:17370:22905 + 1 569612 GCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGT @CCFFBDEHDHHGJIJJGJJIJGIIJJJJIJJIIIJJIGIHGIGEHIGHIHIIJJIJJIHEHIIJIIIGHIIJJHIEEGHGGFFFDEEEE@EDDDDDDDDD 0 11:T>C -AMELIA:165:C03TUABXX:5:1101:13162:14251 - 1 569616 TATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTC :43?:4A>>:A>(BBB@>?A>AB>;(B@DBD?77>B?A?CEEA;C@EEDC@77F@FFF=8)>??DIFIDIGF<F<FG?FF?HEBBIFEFDC<22DDDD8;8 0 0:T>C,93:T>C -AMELIA:165:C03TUABXX:5:1101:3698:42634 - 1 569618 TCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCAC CDC>DDEDDCCDBBBDDDDDDAA@EEEEDEFFFFFD@FHHHHHJJJJIIIHJJJIJIHGIIHIGJJJJJJJJJJJIJJJJJIIJJIJJHHHHHFFFFFC@B 0 2:T>C,95:T>C -AMELIA:165:C03TUABXX:5:1101:2657:34228 + 1 569619 CAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCACA CCCFFFFFGHHHHIJJJIJIJJJIJJJJIGJIIJJJJJIIGGHEGIIJJIJJIJJJJJIJJJIIIJJJDGGIEHEGHHHFFFFEFEECDDDDDACCDDDDD 0 4:T>C,97:T>C -AMELIA:165:C03TUABXX:5:1101:9585:41430 + 1 569624 ATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCACACTTCT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJIHHHHHHFFFFEEEDEDDDDDDDDDDDD 0 92:T>C -AMELIA:165:C03TUABXX:5:1101:19901:25462 - 1 569626 TAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCACACTTCTAG CA>DDDDDDDEDDCCCDDEEDDDEEEEEEEFFFFFFHHHGHHEIJIHJJIIJIIJJJJJJJJJJJIJIJJJJJJJJJJJJJJHFJJJJHGHFHEFFFFCCB 0 10:T>C -AMELIA:165:C03TUABXX:5:1101:11036:30629 - 1 569626 TAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCACACTTCTAG DCADBDBDDDEDDCDDDDEFDDDEEEDEEEFFFEFFHHHHHHHJJJIJJJIJJJJJJJJJJIJJJJJIJJJJJJIJJJJJJJIHJJJJHHHHHDFFFFCCC 0 10:T>C -AMELIA:165:C03TUABXX:5:1101:14716:54932 - 1 569630 CTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCACACTTCTAGTAAG @BBDDDEDC>@@DDEFDDDDDDCECEEEEEFFFFFFFHHHFHHHJJIJJJJJJJJIJJJJJJJIJJJJIJIJJJJJJJJIJJJJJIHGHFHHHFFFFFCCC 0 14:T>C -AMELIA:165:C03TUABXX:5:1101:16785:42029 - 1 569640 CACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTGCGTTTTCACACTTCTAGTAAGCCTCTACCTG A>>@:>::((A@>5;A@3@:BA>A;(>55;3;;;6(;A>DB@?;;EFEEEF;'EEDBB?9GB8)00?:))1FFF<<A,A<HF?FBF>EA::3:4;DAB?== 0 24:T>C,31:A>G -AMELIA:165:C03TUABXX:5:1101:3246:45517 - 1 569642 CTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCACACTTCTAGTAAGCCTCTACCTGCA DDDDCDCCDDCDCDDDDDDDDCCCDDDDDEEEECCFFDFFFHHHIJJJJIJIJJJJJJJJJJJJJHIIJIJJJIHFHHJJJIHJIJJJHHHFHDBFFFCCB 0 26:T>C -AMELIA:165:C03TUABXX:5:1101:5732:18678 - 1 569643 TTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCACACTTCTAGTAAGCCTCTACCTGCAC CEDCDDDDDDDDDDDDDDDDDDDDDDDEECEEEEEFFFFFFHGJJJJJJJJJJJJJIIJJJIIJIIHJJJJJIHHHHJJJIJJJJJJJHHHHHDFFFFB@@ 0 27:T>C -AMELIA:165:C03TUABXX:5:1101:3328:32042 - 1 569643 TTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCACACTTCTAGTAAGCCTCTACCTGCAC CEEDDDCDECDCDDDDDDDDDDCDEDDDD@EEECEFFFFFFHHIJIGHF<JJJJJJIGJJJJIJIGGJJJJIIHFGHJJIJJJJJJJJHHHHHDFFFF@C@ 0 27:T>C -AMELIA:165:C03TUABXX:5:1101:18524:8870 - 1 569649 TCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCACACTTCTAGTAAGCCTCTACCTGCACGACAAC 3A>>>:C>@:AA@CA>>CCCCA;,:553CC;DCA;68B==8EAEAGHCIHHBGDIHFBB?:AB9DDAGHHGIGIHHGGHEDGEFCHDGFFFDHDDBAD??? 0 33:T>C -AMELIA:165:C03TUABXX:5:1101:9362:10230 + 1 569655 CAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCTTTAATCCAAGCCTACGTTTTCACACTTCTAGTAAGCCTCTACCTGCACGACAACACATAA CCCFFFFFHHHHHJJJIIJJIJJJJJJJJJJIGJIGAGGIEHIJIJHJJJJJJJJJJGHJIJJJGIGGIGIHEHHGHFFFFFDEEEEDDDDDDDDDDDDDC 0 40:C>T,61:T>C -AMELIA:165:C03TUABXX:5:1101:6211:23747 + 1 569768 CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCAC CCCFFFFFHHHHGJIJJIJJJJJJJIJIJJIIIIJJJJJIJJIJIIJJIJJIJJJGIJJJJHHHHFFFFFFCEDDDBDDCDDDDDDDDEEEEDEDDDDDDD 0 34:G>A -AMELIA:165:C03TUABXX:5:1101:13419:28650 + 1 569768 CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCAC CCCFFFFFHHHHHJJJJJHIJJJJJJJJJJJJJJJJJJJJJJJFJJJJJJJJJJJJJJJJJHHHFFFFFFFDEDDDDDDDDDDDDDEDEEEEDEDDDDDDD 0 34:G>A -AMELIA:165:C03TUABXX:5:1101:11682:49626 + 1 569768 CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCAC CCCFFFFFHHHHHJJJJJHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHFFFFFFEEDDDDDDDDDDDDDDDEEEEDEDDDDDDD 0 34:G>A -AMELIA:165:C03TUABXX:5:1101:11644:57057 + 1 569770 CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTC C@@FFEFFHGHHGIIIHIJJIJJCHIJIIIFGGIIJIIEHJIJJJIJJJIJIIGIGJIEAEEHGFFFFFFFD>AB?/:CCCDDC>@CCDDDDDCDDDDDDD 0 32:G>A -AMELIA:165:C03TUABXX:5:1101:21135:16926 + 1 569775 CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAA @@@BDDFDHHGHHBFEFH?G9BEFEGG+AFHGHIGIIGEGIGGIGGIGGEGEDAGGIGEFHCHHGDFB<BB@CCCC>CACC:C@A:,>AACCCCCCCAA@C 0 27:G>A,98:C>T -AMELIA:165:C03TUABXX:5:1101:9242:28215 + 1 569775 CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAA CCCFFFFFHHHHHJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHGHHHHFFDDDDDDCDCDDDEDEEEEDEDDDDDDDDDDDDDC 0 27:G>A,98:C>T -AMELIA:165:C03TUABXX:5:1101:1523:44636 + 1 569775 CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAA :??D=DD;CFFFDIF@>FFIC@AF;+93AF):E)9C3*?DGE@D?==BFFFIBF@7;CCE>:?EAB99''3;5?A(5>A###################### 0 27:G>A,98:C>T -AMELIA:165:C03TUABXX:5:1101:17619:26812 + 1 569777 ATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACG =?<DBADBA:?8FGG?BBB;FHGIB@GGH?HECGEHC;0@G6B8B;C=CH=.=E@EGGEBEEHCD44<>3;?AC3::5@;@A@>@5:>C@CCCCCAA@### 0 25:G>A,96:C>T,100:C>G -AMELIA:165:C03TUABXX:5:1101:15058:59676 + 1 569777 ATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACC CCCFFFFFHHHGHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ9D############################################# 0 25:G>A,96:C>T -AMELIA:165:C03TUABXX:5:1101:4115:20272 + 1 569820 CTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACC CCCFFFFFHHHHHJJJJJJJJJIJIJJJJJIIJJHHHIJIJGHIJJJJJIJJJJIJJJIIJIFHHHHHFFFFFEEEEECDDDDDDDDDDDDDDDDDCDEEC 0 53:C>T,57:C>G -AMELIA:165:C03TUABXX:5:1101:14483:11788 - 1 569824 GCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATG CCBCACCEECC?8CCBCCEEEEFFFFFHHHHGGIIHE@IIIHF=IIIIIIIHIIIHIIGIGHDIIIIIIGIHIIIHIIIGFCIEHHFBHHHHHAEFFFC@@ 0 47:C>G,51:C>T -AMELIA:165:C03TUABXX:5:1101:4090:59392 - 1 569830 CTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCG DEECCC?DDBDDDDBDDEBECEFFFFFFHGHHHHEHC=HHJIIHGGIIIGIHGJIHGIHJJJJJJIIGIHJIHGHHHHGHIIIHGEHGHHHHHFFFFFCCC 0 53:C>G,57:C>T -AMELIA:165:C03TUABXX:5:1101:1735:29040 + 1 569831 TAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCGC CCCFFFFFHHHFHJJJJJIJJJJIIGIJJJJJIJJJJJJJJJJJJJJJJJJJJIJJJJJHBHIJHHHHGHFFFFDDDDDDDDDDDDDEEDDDDDDDDDDDB 0 42:C>T,46:C>G -AMELIA:165:C03TUABXX:5:1101:12107:13677 - 1 569839 TCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAAC 5BBCCBBCCBCCCCCCCAADCA@CCCCBCCEEEEDE=HGDFIHCHFFCHGCHHIHIHIGHDBHGIEGCGGHIIIGIIHFFIHH@EIIGHHFHHDDDDD@?@ 0 62:C>G,66:C>T -AMELIA:165:C03TUABXX:5:1101:11430:50122 - 1 569839 TCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAAC ########?<=5(AA>:;>;AA>AA>@>.;@DA??D<A@;)@@@CBB.C>CDAIED??900DDED@DADC:?1D?E?>AA?2?C:EEADDD7DBDDDD=?? 0 62:C>G,66:C>T -AMELIA:165:C03TUABXX:5:1101:5765:26401 - 1 569849 CCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGC DDCDDDDDDEDCADDCDBCDDDDDB=<DFFEHHEEHG@IJJIIHIIHHEF;IHHDHGCGFBJJIIEGHDFJJIJIJIGJJIJIIJIHIFHHDHFFFFFCB@ 0 72:C>G,76:C>T -AMELIA:165:C03TUABXX:5:1101:14629:63588 - 1 569853 GTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACAT A@DCC>:A:DDDDBDB>CAA8?9CB?CFE?CEEAHIGGGGGF@;=(6C=C??/FDGHGFIG@BFB:JIIF@GHGIJJGIHJGHGEFFAHHGHHFFFFFCCC 0 76:C>G,80:C>T -AMELIA:165:C03TUABXX:5:1101:4943:43295 - 1 569861 ACTTCCACTCCATAACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGC @DDDDDCA@CCDDB?DDDCEEDEDC?FDCEFGFHHIIGGJIHHFCCBFFGDJJIGFB?GIHGIFGGGJIIJIJJIJIIIGIJJJJJJIFHHHHDFFFFCC@ 0 84:C>G,88:C>T -AMELIA:165:C03TUABXX:5:1101:5526:35590 + 1 569863 TTCCACTCCATAACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCA CCCFFFFFHHHHGIIIGIJIJIIIJJJJIIJIJIJJJJJJIIJIJJIIJJJJIJJJIJIJIJGIJJHEHFCDDAADDDCDBBDDDCCDDDDDDDDDDDDDD 0 10:C>T,14:C>G -AMELIA:165:C03TUABXX:5:1101:20118:42737 - 1 569864 TCCACTCCATAACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCAC >B?:BCDDBB?8DADCDECD@>@EBFEBEEA?EAC@JHF@@8F@F@B8IGHCFDBJGGIGIGGHFGIJIJJGGIFCHIJJJJJJJHHIHDCHHFFFDD??@ 0 87:C>G,91:C>T -AMELIA:165:C03TUABXX:5:1101:7710:64308 + 1 569876 CGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCT CCCFFFFFHHGHHJJIJIJJJJJJJIJJJIIJJJIJIIJJJFJIJJJJIJIIJJIIJIIHHHHHFFFEDDDDDDDDDDDDCDCDDDDBDDBDDDDDDDDDD 0 1:C>G -AMELIA:165:C03TUABXX:5:1101:10716:25363 + 1 569877 GCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTG CCCFFFFFHHHHGJIJJJGIJJJJJIJJIJIHIIGJIJJJIJJJJJJIFHIJIIIJIJIHFHHHHFFFDDDDDDDDDDDCDDDBDDDDDDDDDDDDDDDDD 0 0:C>G -AMELIA:165:C03TUABXX:5:1101:19856:23377 + 1 569881 CTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCA C@CFFFFFHHHHHIIJJJJJIIJJJIIIIIJIIJJJJIIGHIIIJJJJJJJJHIJCEHIIIHHF:?EECEEDDDDDDDDDDDDDDDDDDDDDDDDDDCCDD 0 -AMELIA:165:C03TUABXX:5:1101:8824:59392 + 1 569881 CTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCA @<@DDDD;ACFFBFGEEHIG;CF@BFFHEBEDEF<C<DHHECFCDB?FHEAA;AG=@DHGIH=D?BCC@;AC@>@CAAB??BBBB@B<?@@????@C@CCC 0 -AMELIA:165:C03TUABXX:5:1101:11504:61968 + 1 569881 CTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCA CCCFFFFFHHHHHJJJJJJIIJJJJJJJJJJJJJJIJJJGIJJJJJJJJJJJJJJHIIJJJHHFFFEEDEEDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD 0 -AMELIA:165:C03TUABXX:5:1101:1304:19589 + 1 569889 AGGCCTACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCC CCCFFFFFHGHHHJJJGIIJJJIJCHFHGIHCHIIEEGIEGEEGGHIFHIGJIIIGHHHGFFFFFCECEEBBBDBBBADBDDD@DDDBDACDDDCDDD??A 0 93:G>A -AMELIA:165:C03TUABXX:5:1101:5690:51586 - 1 569889 AGGCCTACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCC DCDCCC>DCBBDBC?CAA@ACCCCCCACA;CCEEEEFFHGJJIGGGIGIGHGIJIGIJJJIIJIGGJJJJJIIHGIEGFFF@GC<IHGFHGHHFFFFFC@C 0 7:G>A -AMELIA:165:C03TUABXX:5:1101:21117:4467 - 1 569893 CTACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCG DDABDBBBBCBCCCCBCDDDEFDDDDDDDCCECDDFEFE@DIIGIGGGIIIIIIIIHFIIHFIIIIIIIHFGGFGEGCHC<IIGIIIIHHHHGFFFDB@@@ 0 11:G>A -AMELIA:165:C03TUABXX:5:1101:2553:15831 - 1 569893 CTACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCG EECDDB?DDDDDDDCBAC@DFFDDDCDDEDDEDDDFHHHJGGJIJIIHJJJJJJJJIIIIIGJJJJJJIHGIHFGEG@HF?IIIJJJJHHHHHFFFFFCCC 0 11:G>A -AMELIA:165:C03TUABXX:5:1101:12504:48011 + 1 569895 ACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGAT @CCFFFFFHHHHHJJJJJIJJJIJJJJJJIJIJJJJIJJJJHIJJJJJJJIJJJHHHHHHFFFFFDDDDDDDDDDDDDDDDDDCDDDDDDDDDDDDDDDDD 0 87:G>A -AMELIA:165:C03TUABXX:5:1101:18316:51199 - 1 569895 ACTAACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGAT C@C?<BBCBCD@A@>A:CDEECCCC>ECCCBCCCEHHHHGGBIHIHGCHIIIIHEFIGHFIIFIIIGGIIHGHGGGHF<IHCG<FIIIHHFFHDDDDD@@@ 0 13:G>A -AMELIA:165:C03TUABXX:5:1101:2349:27113 - 1 569897 TAACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATAC >BBB@CCCC>>BCC>CDCDAC:ACDCCCCBBCCEDCHHHECGGGHGGIIIIIHFGIHFGGIIGFHF?=GFD??0HBDFBHHGHGGIIIHFFAHFFFFD@@@ 0 15:G>A -AMELIA:165:C03TUABXX:5:1101:9409:29993 + 1 569898 AACCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACG CCCFFFFFHHHHHJJJJJIIJJIJJJIJJJJJJJJIJJGIHGJIJJJJJJJJJJHHHHHHFFFFDDDEDDDDDDDDDDDDDDDDDDDDDBDDDDDDDEDDD 0 84:G>A -AMELIA:165:C03TUABXX:5:1101:18137:4904 - 1 569900 CCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGACACGGG @DDBCDDCDDCCDEEDDCCDDEDCDBDDDDDDFFFFFFHHHHJJIJJJIIGIJJIJJJJJIIHGGGBGBG@HF?JIHIIJJJJJJJJJHHHHHFFFFFCCC 0 5:T>C,18:G>A -AMELIA:165:C03TUABXX:5:1101:8262:35274 - 1 569900 CCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGG ?DDBDDCABCDCCEFECC@DDDCDDDDDDDDDFFFFFFEEFHJJJJJJIFFIIHHJJIJJJIHFIHFGBGCHFDJIHIGIIJJJJJJJHHHHHFFFFFCCC 0 18:G>A -AMELIA:165:C03TUABXX:5:1101:8681:42267 - 1 569900 CCAACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGG DDCBCCDDDCC@DEDDDDCDDEDDDDDDDDDDFFFFFFHHHHIIIJIJIIHJIJHJJJJJJGHGIHFGFG?HGCJIIIIJJJJJJJJJHHHHHFFFFFCCC 0 18:G>A -AMELIA:165:C03TUABXX:5:1101:6697:56559 + 1 569902 AACACACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGGAT ?@@DDDDDHFFHDIIGIICGEHEGIIGIIIFIGIHGGG@GEBDEGIIIGCFAD@@CEHHE??DDB=AABB=AB@ACACCDDDDBB?BBCC>ABDD<@B@<5 0 80:G>A -AMELIA:165:C03TUABXX:5:1101:13952:39077 - 1 569907 ACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCC CDCC@DDDDCC>ACDDDDDBDB@DBDCEECA??DDFHFHHHC?IIIHCIEIGHFF;8EF?F?B?HFBGHGGEIIIIIHGGGEGBIIHGGHGHGFFFFFC@@ 0 25:G>A -AMELIA:165:C03TUABXX:5:1101:1405:59406 - 1 569907 ACTAACCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCC :A@C>DDEDDCCDDEDDDDDDDDDDDEEEEA=FBFFHGHHHGHHJIGEJJJJIHHHFHFBD?G@GD?IHGHFJJIJJJJJJJIIJJJHFHHHHFFFFFCCC 0 25:G>A -AMELIA:165:C03TUABXX:5:1101:21295:35040 + 1 569912 CCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTT CCCFFFFFHFHHFIJJIJJIJJIJIJJJJIEHEHJJIIJJIIJG==FGGIGIJJJHHHHFFFDDEEDDDDDDDDDDBBCC@BBEDB?BDB>CCDD:ACDDD 0 70:G>A -AMELIA:165:C03TUABXX:5:1101:3951:60121 - 1 569912 CCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTT CEEEDDCDDEDDDDDDDDDDDEDDCBCDCDFFFFFFFHFHHHHJJJJIJJIGJHFHFF?HD?JIGHGJJJJJJJJJJJJJIJJIHJJJHHHHHFFFFFCCC 0 30:G>A -AMELIA:165:C03TUABXX:5:1101:16218:59305 + 1 569927 GGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTT CCCFFFFFHHHHHJJIIIHIJJJJJJJJJIIJJJJJIJJJJJJIJJJJJJJGHHHHHFFFDDDEEDDDEDDDDDBDDDDDDDCDDEEEDEDDDDDDCCDCD 0 55:G>A -AMELIA:165:C03TUABXX:5:1101:19217:38022 + 1 569941 CGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGG CC@FFFFFGHHHHJJHJJJJJJJJJJJJJJJIJJIJJIIJJJJIJJJJJJJJJJJJJHHHFFFFDEEEDEEEFEDDDDDDDDDDDACEDDDDDDDDDDDDD 0 41:G>A -AMELIA:165:C03TUABXX:5:1101:13157:14781 + 1 569943 AGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGAT CC@FFFFDHHGHHHIDGGHHHGIIGJJGI?GGGGIDFG?BGGAHG=C=BCFIDHEDGB:5)7;@CFE@CDC@CCC>CCAAC@A:>@CDB<CCCDDD@D5?C 0 39:G>A -AMELIA:165:C03TUABXX:5:1101:18829:32029 + 1 569944 GAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATT CCCFFFFFHHHHHJJJJJJJJJJJJJIIJGIJJJJIJJJJJJJJIIJJJJJJJJJJHHHHFFFFFFEEEEEEFEDDDDDDDDACEDDDDDDDDDDDDDDDD 0 38:G>A -AMELIA:165:C03TUABXX:5:1101:15451:19067 + 1 569945 AAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTT @C@FDFEFFFHFFGIIIGGHIGGHJGIEGIIIGFGGHJIIJGJIIIJEIJGGFHGG<ECHBEDFEFDCEEECCCCCCDCCD>ACC@@DDDDDDBDDDBC@C 0 37:G>A -AMELIA:165:C03TUABXX:5:1101:12806:55929 + 1 569945 AAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTT @@CFFFFFDHHHHIGEEHIIIIIJGJJIGIIIIGHGIIICGGGGHIJIIIGIJJIGFEHEFFFFFFEEDEEEECDCCAACD>CDDDDDDDDDDDDDBBDDC 0 37:G>A -AMELIA:165:C03TUABXX:5:1101:19735:15762 + 1 569949 CACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCT CCCFFFFDHHHGHJJIJIJJIGIJJIJIJGIJJJJJJIIIIIJJIJJJJJJIIJGHEHHHEFFEFFFFFEECEEDCDCDDDDDDDDDDDDDDBBCDDDDDD 0 33:G>A -AMELIA:165:C03TUABXX:5:1101:1914:41988 + 1 569949 CACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCT @@CFFFF>FHFDAGHGIIIIGII@FHEGH?DDHGEGGGG9FF8??CBFGIGHGGGEHHGCEFEFFEFFFECCCECCC;@ACBBBCCCCBBBBB?:CC>:CC 0 33:G>A -AMELIA:165:C03TUABXX:5:1101:6355:51422 - 1 569950 ACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTG 4>:@:CC:>??@@<B?@BBBB<:3B?ACCB@A@CCC?;??;BB?A8GEDHF=@GHCEGF@GFGD>FB?HEIIHBBHGGGEEEEHHBEGDFHAF;DD??@@@ 0 68:G>A -AMELIA:165:C03TUABXX:5:1101:9220:50595 - 1 569960 GCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCTTTTAC BDDCDDDBAB<BC@DDCDDDDDDDDDDDDDDDDEEC?DFFHHHHHGJJJIJJIJIFFJIGJJIJJJJJJJJJJJJJJJJJJJJJJIJJHHHHHFFFFFCCC 0 78:G>A -AMELIA:165:C03TUABXX:5:1101:8055:24237 + 1 569965 CACACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCTTTTACCACTC CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJIGJJJJJJIJJJJJJJJJJJJJIIJHIHHHFDEDDDDDDDDDCDDDDDDDEDDDDDDDDDDDDDD 0 17:G>A -AMELIA:165:C03TUABXX:5:1101:17657:6958 - 1 569968 ACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCTTTTACCACTCCAG 8B<BC>DDCCCDDDDDDDDCDDDDDBDBADEEEEEEFFFFFFHHHHHE?JJJJJJJIJIJJJJJJIJJJIJJJJJJJJJJJJJJJJJJHHHHHEEFFFCCC 0 86:G>A -AMELIA:165:C03TUABXX:5:1101:14266:7981 - 1 569968 ACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCTTTTACCACTCCAG 2?5B@4DDDDCDDDDDDDDDDDDDDB@?=BEEEEDEFEFFFFHFHHHEEIGHGIIIGIIIHGIIJJIJIIJJIIJIIJJJIGGBGGIJHHHFFDBDDD@@@ 0 86:G>A -AMELIA:165:C03TUABXX:5:1101:12453:30279 - 1 569968 ACACCCCCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCTTTTACCACTCCAG ###########################??=;@@;;.;73;77=)==)..=@7;ICA=88<?BDADD:C:BBIIIE@FDCEFEA2+;<E4<A:D?D?DD??? 0 86:G>A,95:A>C -AMELIA:165:C03TUABXX:5:1101:3902:48785 - 1 569968 ACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCTTTTACCACTCCAG #?<BC@<<4>>@<CCCC:A987C??<;35B@C@CCCB;@@BEHHHCC=;GEAHEHAHEG>F;HF<HHEGIGGHCHFEFCIHIGAIHE@DFBBHDDB=??@? 0 86:G>A -AMELIA:165:C03TUABXX:5:1101:16690:49177 - 1 569968 ACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCTTTTACCACTCCAG <?<B@:DDDDDDDDDDDDDDDDDDDDDDBDEEDEEEFFFFFFHHGHHECJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFEFFFCCB 0 86:G>A -AMELIA:165:C03TUABXX:5:1101:19110:46499 - 1 569975 CTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCTTTTACCACTCCAGCCTAGCC BCCCDDDDDDDDDDDDDDDDDDDDCDCCDDDDDEEEEEEDD@FFFHHHHHHJJJJJJIJJJJIJJJJJJJJJJJJJJJJJIJJIGHGCHHHHHFFFFFCCC 0 0:T>C,93:G>A -AMELIA:165:C03TUABXX:5:1101:11580:53512 + 1 569982 AAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCTTTTACCACTCCAGCCTAGCCCCTACCC <?=?DDAABBAF?@=GGIGGACHGGEF@GGIIFIIIGFGGIB>G?B<F?AGIIIIG>EFFF<DC>ACCEDC:5>CACCCCCCCCCCCC?<>9@9A@8??<A 0 0:G>A,93:T>C,96:C>T -AMELIA:165:C03TUABXX:5:1101:9621:47112 + 1 569983 AAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCTTTTACCACTCCAGCCTAGCCCCTACCCC <@@A1=ABFFFFDIFEAAGIC9CG>E?CFFAFDD?:?FD@?GFD9BBDFFFCDDEEE4AB@BDBAAA3;6>A9@A@AAABBB5<>>38(49493)8?B<?< 0 92:T>C,95:C>T -AMELIA:165:C03TUABXX:5:1101:13137:32759 + 1 570017 CCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCTTTTACCACTCCAGCCTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAGGCATC @?@D?D;?DHBFHIIA@GBH=:EDB7?BFHI@FB<?D?DDH>?BBG38=7=C@D7=7@==:??D##################################### 0 58:T>C,61:C>T,71:C>T,76:G>A,79:A>G -AMELIA:165:C03TUABXX:5:1101:17894:57779 + 1 570099 CTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACC CCCFFFFFHHHHHJJIJJJJJJJJGGGIIGHHHJJJGIGIJHHGGGHJIJJJIHHGHFFFFFDEDEFDDDDDDDDCDDDD3>CDEDDDDDDCA9(:>4@CA 0 78:G>A -AMELIA:165:C03TUABXX:5:1101:11418:9814 + 1 570100 TGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCA CCCFFFFFHHHHHJIJJJJJJJJJJJJJJJJJJJJIJJJJHIJIJJJJJJIIJHHHHHFFFFEEEEEDDDDDDDDDDDD?CDEDDDDDDDDDDDDDDDDDD 0 77:G>A -AMELIA:165:C03TUABXX:5:1101:10734:32557 - 1 570101 GGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCAT DDDDDDDDCDDDDDDD@9DDDDEECCEDBFHHHHHHIIJIIIHFJJJJJJJIIHJJHJJJJJIHFJJJJJJJJJJJJJJJJJJJIGCJHHHHHBFFDFCC@ 0 24:G>A -AMELIA:165:C03TUABXX:5:1101:3724:15102 + 1 570104 CCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGT CCCFFFFFHHGGHJJJJJJJJIJJJJJJJJJJIJJJGHIJJJJJJJJJJJJJJJJJHHHHFFFFFFDDDDDDDDDACDEDDDDDDDDDDDDDDDDDDDDDC 0 73:G>A -AMELIA:165:C03TUABXX:5:1101:12820:59050 + 1 570104 CCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGGGCTCACCATAGT CCCFFFFFHGHHHIJJJJJJJJJJJIJJJJJGIIJJJIJJJJCHIIJJIGIJJIJJHHHHFFFFFFDDBDDDDDD?ACEEDDDDDDDDBDDDDDDDDDDDA 0 73:G>A,88:A>G -AMELIA:165:C03TUABXX:5:1101:2140:58964 + 1 570107 CAACAGGCATCACCCCGCTAAATCCCCTAGAAGCCCCAGTCCTAAACACAGCCGGGTCACTCGCATCAGGAGGATCAATCACGTGAGGTCACCATAGTCTA ??<B;?DDD?;<2A<EE1C1)**1:*1:B)00?*00@D(09B########################################################### 0 33:T>C,38:C>G,50:T>G,54:T>G,55:A>G,57:T>C,70:G>A,72:T>G,82:C>G,87:C>G -AMELIA:165:C03TUABXX:5:1101:6789:26334 + 1 570113 GCATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAA CCCFFFFFHHHHHJJJJJJJJJIJJJJGHJJJJIJJJJJJIIJJJJJJGIJJJJJJIHHHFEFFFDCEEEEDDDDCDDDDDDDDDDDDDDDDDEDDEDDDD 0 64:G>A -AMELIA:165:C03TUABXX:5:1101:17313:33681 + 1 570114 CATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAA CCCFFFFFHHHHHJJJJJJJJIJJJJHIJJJJJJJJJJIJJJGIJJJIJJJJJIJJJHHHFFFFDCEEEEEDDDDDDDDDDDDDDDDDDDDEEDDEDDDDD 0 63:G>A -AMELIA:165:C03TUABXX:5:1101:17071:44350 + 1 570114 CATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAA @@;DDBDBFAFHDEBFGFFGDBFDFD??DBAFHGIA@G?BBFGIIGHIGIIEGIEHGFFBDDCFD.;ACECDCCDCD??CDCDDCA:ACC:ADCC>>A:@@ 0 63:G>A -AMELIA:165:C03TUABXX:5:1101:2963:46573 - 1 570114 CATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAA CA?B<DDDDDDDDDBBDDEEEEEFFDEEHCAIGIJGIG@F@IGHJJJJJIIGJJGJJIJJJJJJJIIIJJIGGCIHGIIIGICGIJJJHGHHHFFFFFCCC 0 37:G>A -AMELIA:165:C03TUABXX:5:1101:7311:34629 - 1 570116 TCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAAC B>9DDDDDDDCCDBDDEEEEEEEDBEFC=HHJJIJJIGCIEGJJIIEHF@F@HEJJIJJJJJIJIJHEGFC<JJIIIHDCGIGGGHHGBGDHFDFEDD?=@ 0 39:G>A -AMELIA:165:C03TUABXX:5:1101:6887:61374 - 1 570116 TCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAAC ?>9B@DDCDDCDDBDDEDEEEEEEFFEHEEEGHAIHGHFHFBIJIGIJIHIIIGBJIJJJIJHGJIJGIHFCJJIJIJJIIBJGJGIHGGHHFFFFFFCC@ 0 39:G>A -AMELIA:165:C03TUABXX:5:1101:12184:14999 - 1 570119 CCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC DDDDDDDDDBBDDDDCDEEECAED@BHAHHFHG@DDIG@JJJHGFB?HCIJJIIIJJHF>GIIIIIIHHIGEJJIIGIHJJIEJJJIFHHGHHFDFDF@?@ 0 42:G>A -AMELIA:165:C03TUABXX:5:1101:5025:23019 - 1 570119 CCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC DBDDDDDDDDBDDDDDDEDDEDFEB>HFHHHFE@HDHGIJJJJJJIHJJJJJJJJJJJJJJJJJJJHGAJIIJJJJJIGJJJJJJJJJHHGHHFFFFFCCC 0 42:G>A -AMELIA:165:C03TUABXX:5:1101:4269:56988 - 1 570119 CCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC DDDDDDDDDB=BDDDDDEDEEDFEBFHEHHGHIHGDIIGJJJIJIHDIJJJJIIJIJIJIJIGIGJHGCJJJJJJJJJHJJJIIHGJJHHGHHFFFDFCBC 0 42:G>A -AMELIA:165:C03TUABXX:5:1101:7753:9624 - 1 570121 CCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCG DDDDDDCDDDDDDDDDDDDDDDB@FFFFFHHEHEHIHJJJJJJIIJJJJJJJJJJJJJJJJIJIHF?JIJJJJJIIHJJJJJIHJJJJHHHHHFDFFDCCB 0 44:G>A -AMELIA:165:C03TUABXX:5:1101:20344:50879 + 1 570123 GCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAA CC@FFFFFDHHHFGGEGEHGHIGHHIIIHJIJJGGIEIGHHIIJIHIIGHJCEFGE=FGHIIGGCCEEEEFFFFEEEDEECCADDDEDDACDDCDD@@99B 0 54:G>A -AMELIA:165:C03TUABXX:5:1101:9419:42706 - 1 570125 TAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAAC DDDD@DDDDDDDDDDDDDBCDEEECCEDEEFFHHHJJJIHGJJJJJJJJJJJJJJJJIIIHGBJJJJJJJJIGJJJJJJJJJJJJJJJHHHHHDFFFFCBB 0 48:G>A -AMELIA:165:C03TUABXX:5:1101:10571:17700 - 1 570129 TCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAA @9B@CCCCC<?9?@95?;;;>;>6;6<=;BEECA?=4E=FAFF<CBBCIIHDFEGB@?9HFAHGFDFFC>CFFFCEGHGGIHHGCA8E?F?DDBA=DD@@? 0 52:G>A -AMELIA:165:C03TUABXX:5:1101:2817:10466 - 1 570130 CCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAAT DDDDDDDDDDDDDBADDDDDDDEECCDFFFHHHHHEJJJJJJJJJJJJJJJJJJJHG?JJJJJJJIHGJJJIJIJJJJJJJJJJJHJIHHHHHDDFFFCC@ 0 53:G>A -AMELIA:165:C03TUABXX:5:1101:2472:20225 - 1 570130 CCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAAT BBCCACCCBBBBB?5CCCCCC?;>6=A?FEHEEHE=E;HCGGEEIIGHHCGGBBBBB9GHG@HBIHFCGD?9GIIHAGHGHGGGGCIIHFF?DDDDD?@@? 0 53:G>A -AMELIA:165:C03TUABXX:5:1101:17927:59744 - 1 570130 CCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAAT DDCDDDDDDDAB@<9DDDDDDDDEEBC@FFHHHGHEJJJIJJGGIJJJJJIJJIIGB9GIHJHIIHGDJJHEIGIJIJIIJJJJHGJJHHHHHDDFFFCC@ 0 53:G>A -AMELIA:165:C03TUABXX:5:1101:19433:16738 + 1 570132 CCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAA @CCFFFFFDHHHHJJJJJJJJJJIJJJJJHJJJIJJJIJIJIJHIIJGHIJJIJJIIJJJJJJIJIHHHHHFDFFFFFDEEEDEDDDDDDDDDDDDDDDDD 0 45:G>A -AMELIA:165:C03TUABXX:5:1101:17206:53665 - 1 570134 TAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATT DDDDDDDDDDCDDDDDDDFDDDDDDFFFFFHHHHJJJJJJJJJJJIJJJJHGGDJJJJJJJJIGJJJIJJJJJJJJJJJJJIJIHJJJHHHHHFFFFFCCC 0 57:G>A -AMELIA:165:C03TUABXX:5:1101:4423:19063 - 1 570135 AGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTC CDCDB@BBA<CCCDDDCBC@DDCDFFFFFFHHIJJJIJJJJIJJIJJIJIHGFJJJIIHJHHDJJGFJIJJJJJJJJJJIGGHDIJJJHHHHHFFFFF@@@ 0 58:G>A -AMELIA:165:C03TUABXX:5:1101:17543:10747 + 1 570139 GTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGC CCCFFFFFHHHHHJJJJJJJJJIJJJJJJJJJJJJJJJJJFHIJJJJJJJJJJJJJJJJJJJJIJHHHHHHHHFFFFFDDDDDDDDDDDDDDDDEEEDEDD 0 38:G>A -AMELIA:165:C03TUABXX:5:1101:11792:18654 - 1 570142 CCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACA DBCDDDDDDDEDCDDDDDEFFEA?FFHHHJIIJJJJJIJJIIHFEBJJJIJJIHFBJJJJJIJJJJJJJJJJJJJIHJJJIHGIJJJJHHHHHFFFFD@C@ 0 0:T>A,65:G>A -AMELIA:165:C03TUABXX:5:1101:18955:22926 - 1 570143 CACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACCG ##B>AB@@:5>5=B=B?@5B@;,DB9EA?77;EEED@=;F@F;EFB=)D??@>FB>BBFFGEF?EGD>DDFAFGEBIIHG@GD@EFEBFFF>FDDA:A8=8 0 1:T>C,66:G>A -AMELIA:165:C03TUABXX:5:1101:17221:7620 + 1 570159 CGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTCATTACAATTTTAC CCBFFFFFHHHHHJJJJJJJCHIJJJJJJJJJJJJJJJJJJJGIIIIJJJJIJJJJJIJJJJJJHHFFFFDEEEEEEDEDDDDDDDDDDDEEDDDDDDDED 0 18:G>A,87:T>C -AMELIA:165:C03TUABXX:5:1101:21215:55317 + 1 570160 GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTCATTACAATTTTACT @@@FFFFFHDHFHGGIIIICEHIGCH4CEHGGAFGGGIICHGICGFHDHICGGIIIIGGGGIBHHHDFFFEEDDECEDCDBDCDCDCDDEEDDCDDDDDCC 0 17:G>A,86:T>C -AMELIA:165:C03TUABXX:5:1101:16205:21510 + 1 570166 CTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTCATTACAATTTTACTGGGTCT CCCFFFFFHHHGHFGIIJJJJJJJJJIJIJJJJJJJJJDHIIJIIJIJJJJJJJJJJJGIJHHHHHFFFFFFFDEEEEDDDDDEEDDCDDDDEDDDDDCCD 0 11:G>A,80:T>C -AMELIA:165:C03TUABXX:5:1101:5952:43843 - 1 570175 GGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTCATTACAATTTTACTGGGTCTCTATTTTAC DDCDEEDDEDDC@EEEEEFFFFFFHGGHHHIGJIIIJIIIGIHFJJJJHFJIJJJJJJJJJJJIHGJJJJIJIJIIJJJJIJIGJIJJHGHGHFFFFFB@@ 0 29:T>C,98:G>A -AMELIA:165:C03TUABXX:5:1101:9546:33470 + 1 570179 TATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTCATTACAATTTTACTGGGTCTCTATTTTACCCTC @@@FFDFEHFDFDIIHIGGHIFFDGEFAEHICEIGIGIJIGIGIGHIIIIIGIJIJIECHGHIGGECHAHHFFFDBCC@BCEC@B?ACDDECDADCCCCCB 0 67:T>C -AMELIA:165:C03TUABXX:5:1101:19042:8847 + 1 570182 CAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTCATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA CCCFFFFFHHHHHJJJJJIJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJIIJJJJJJJJJJJJJJHHHHHHFFFFFFFEEEDCCDDDEDEEEEDDDDDDDC 0 64:T>C -AMELIA:165:C03TUABXX:5:1101:2207:7298 + 1 570183 AATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTCATTACAATTTTACTGGGTCTCTATTTTACCCTCCTAC @<@DDDB?FFFHHB>@;FIGHFHGGECCDEGHEGGGHIIDGEIHIIIHFCHHBFAACGEG;@GHICHFHCFEEEEB7@>3=.6;ACDCEDD>AC>CA@CCC 0 63:T>C -AMELIA:165:C03TUABXX:5:1101:8324:44377 + 1 570186 CACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTCATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAG CCCFFFFFHHHHHJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJGIIJJJJJJJJJJJIIJJJJJJHHHHHHHFFFFFACEEEDDEEEEDDDDDDDDDDDC 0 60:T>C -AMELIA:165:C03TUABXX:5:1101:19895:16131 + 1 570188 CCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTCATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCC 8?:BD3:D?DD>D4EEAEE?E?>BFECFEDEEDEADADDCAB<B/??BD@8BB==78.==C@DEA;=CDDCA)7@@A@AA>(6;6>;>AAA??=>>AAA25 0 58:T>C -AMELIA:165:C03TUABXX:5:1101:9882:16753 + 1 570188 CCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTCATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCC CCCFFFFFHHHHGJJJHIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJIJJJJHHHHHHFFFCCEEEEEEEEEDDDDDDDDDDDDDD 0 58:T>C -AMELIA:165:C03TUABXX:5:1101:10521:32789 - 1 570190 TGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTCATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTC CDDDDDDCDDDDDDDEEEEEDEEECCFDAHHIHHDJJJIGGJIJGJJJIHFIHJIJJIIHFIJJJJJJIJJJJJJJIJJJJJIHEJJJHHHHHFFFFFC@C 0 44:T>C -AMELIA:165:C03TUABXX:5:1101:17981:7163 - 1 570191 GAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTCATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCA DDCDDDCDDDDDDDEEEEEDDDEDCCEBHHHHIGJJJIJIJJIHJJJIHFGGIJIIIIIDJJJIIJJGJJJJJIIIJJJJIGGCJJJGHHHHHFFFFF@CC 0 45:T>C -AMELIA:165:C03TUABXX:5:1101:13009:5089 - 1 570197 ACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTCATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTAC CDDDDDDCEEEEDDDDDD@BDDFFEBHAHHHJIHIIHCGIJIHGIJJIIJJIIHJJJIJJJIJJJJJIHDIIJJJIHFJIJJJJIHJJHHHHHFFFFFB@C 0 51:T>C -AMELIA:165:C03TUABXX:5:1101:2138:25924 + 1 570198 CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTCATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACT CCCFFFEFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJIJJIJJJJJJJJJJJIJJJJJJJIJJJJJCHHHHHHHFFFFFFEDDEDDDDDDDDDDDDDCDDE 0 48:T>C -AMELIA:165:C03TUABXX:5:1101:7048:43352 + 1 570200 ATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTCATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTC @@@FFFFDBDFHBBG@GGIIDIGHGGIHGIIIDIIFGGII@FGIGGHHIIGHBEGDHEGHIEIJ@DGHFFCEHGFDFFDDDDC>ACCBBC?CDDCC@C>CC 0 46:T>C -AMELIA:165:C03TUABXX:5:1101:3767:39969 - 1 662228 AACTTGAGGAGGCCTGGCCTCTGCCTCCCGCATTGCCCAGCTGTTCCTCCTGGCTGCATCTCCCACCTCCCAGCAAACAAGCTCTTTTGGCTCAGCTCCCG CDDDDDDDDCDBBBDBDDDDDDDBB>D@@?;CDD?CC@DFDDBHHHGHEGHFDGHG@@@IGIHCDIGIIIIJIGIJIJIEIHJIIJJJHHDHHFDFFD@@B 1 -AMELIA:165:C03TUABXX:5:1101:2464:49300 - 1 326273 CAACCTCTTTGGACTCAGTGCCTACCCATCTCCTGGCGGCATTGGTCGGCCCACAGCTTCCTCAAGCCAAGCTCCCCAGGCCCAGGTCAGGCCTCACGGTG A?@A?99CCCCCCCCCCBCCB?>3A@C>1@??-BBB@>9@>BBCA>FHGIEACHGEEC=;FBEIIIHGGEHEBIIIGFEEF@EIGGBEFFHBDDADDD@@; 0 60:C>A -AMELIA:165:C03TUABXX:5:1101:3031:11063 + 1 326249 CCAGGCCCGATTCCTGCCTCTCAACAACCTCTTTGGACTCAGTGCCTACCCATCTCCTGGCGGCCTTGGTCGGCCCACAGCTTCCTCAAGCCAAGCTCCCC @@CFFFFFDDHFFIGGIJJGIGIGEEGHIGIGGCGFEHIIBFDGGHCGIJIDGGIIGGD=DEF>B@DDCCCB:B@<?B<BBDDDDCCCCCC?C?<?@CCDB 0 -AMELIA:165:C03TUABXX:5:1101:7628:28013 - 1 663374 GACGCATCCAGGAAGAAGAGCTGGACCCGGAGAGGACGCCCGGAGGGTGCAAGTGGCTCTGGAGAGGCCGACTTGAGGAGGTTCTGGGCCCGGAGAGGCCG ############################B?A>BB@;/?:=/=;?DEA?=)))EAC@=@7F@DF>EF@8B?0?GD?IFC@=DG=BFC8<@<:;ADDD?D?=@ 1 44:G>C,66:A>G,76:G>A,94:A>T,97:C>G -AMELIA:165:C03TUABXX:5:1101:6797:10552 - 1 576483 AGGAGAGACTGGGTGGGTGCTATAAGTGGGGGTGGGCATATAAAGGCTGCTGTTCATCTTTCCGCCTTGGCTTCCCAAAGTGCTGGGATTACAGGCGTGAG ##################################################################C3;E?C383EE>B@GC::F>EFC22<?DD?D?1?? 0 29:C>T,38:T>C,40:C>T,41:G>T,46:G>C,48:G>T,49:C>G,52:C>G,53:A>T,54:G>C,55:T>G,56:C>G,57:C>A,58:T>A,59:C>A,62:C>T,63:C>A,64:T>C,65:C>G,66:T>G,68:G>T,69:T>G,70:A>G,73:A>G,74:C>T,76:G>A,79:T>A,80:G>T,81:T>C,84:C>G,85:A>G,86:C>G,88:T>G,89:T>G,92:G>C,93:T>A,94:C>G,99:A>G,100:T>A -AMELIA:165:C03TUABXX:5:1101:8743:50636 + 1 142312 GCAATATGTGGTGCTCATGAACAAAGCAAACATTAAAGTCAGACCAGACCCAACATTTGACTCAGTCTTAATATCCAGGTGAGCCTGCGCAAATCATTCAT CCCFFFFFHHHFHJJJJJJIIJJJJJJJJJJJJJJIJJIJIJJJJJJJJJJJJJJJJJJJJJIJJJJJJIJJJJHHHHHEEDEFFEEDDDDDDDDCDEEED 1 -AMELIA:165:C03TUABXX:5:1101:15871:55839 + 1 682091 GGGAAGGCTGACATGGTAGGATTGCTTGAGCCTGGGAGTTTGAGGCTATAATGAGCCATGATAGGACCACTGAACTCCATCCTGAGTGACAGGGCAAGGTC @C@FFFBFHHHHHIIFCFEEFGHGHHIIJJJJGIJJEHDFHIEHIIIEHIJIIHEGFHGEGHIIGGGHFFFEHDFDDEEEEEEECDCCDDCCBBD?BDD:> 1 -AMELIA:165:C03TUABXX:5:1101:6309:29014 - 1 146406 AGGCTGACATGGTAGGATTGCTTGAGCCTGGGAGTTTGAGGCTATAATGAGCCATGATAGGACCACTGAACTCCATCCTGAGTGACAGGGCAAGGTCCTGT ########@52;>;;;@;>>@?@AFEECAEC@@7=AFF>FGF<CIFD9D9)?*B909????:DGCFFGBG?;;BIG?GFE?C@EAEC2<:<+FB;DAB@?? 1 -AMELIA:165:C03TUABXX:5:1101:13303:47126 - 1 682180 CAGGGCAAGGTCCTGTTTCTGAAGAAAAAAAGGACATTGGAATCAGGACCCTCTCCATCCTGAGGTGCCTACAAGGCATCTCTCTCTGCAAACGAGTAAAC DDDDDDDDDDDDDDDDDDDEDDDDDDFFFHHHHHHHJIIHJJJJJJIIJIJIJJJJIGJIJJIJIJIJJJIJJJIIJIJJJJIIJJJJHHHHHFFFFFB@B 0 -AMELIA:165:C03TUABXX:5:1101:11676:33354 + 1 149508 CTCTACTAAAAATACAAAAATTAGCCGGGCATGGTGGTGGGGCCCTGGAATCCCAGCAACTCGGGAGGCTGGGGCAGGAGAATGGCGTGAACCTGGGAGGC ?;?+4AA?DFBH>?FAFIEID<<F;2<:):?DGH*0?0?D############################################################# 1 25:T>C,29:T>C,37:C>T,41:C>G,42:A>C,47:T>G,57:T>A,71:A>G,83:T>G,86:T>G,93:C>T,94:A>G,96:A>G,100:T>C -AMELIA:165:C03TUABXX:5:1101:11918:52845 - 1 700492 AAAAGAAGAAAAAACCCACTCAAAAAAAAAAAAAAAAAATTCCTTTGGGAAGGCCTTCTACATAAAAATCTTCAACATGAGACTGGAAAAAAGGGTATGGG ######################@DD@DDDDDDFHJGJJJJHIIIIIIJIGGIGJJJIGD?GEIIJJIIJIJIIJIIGIIIHHIJJJJJHGHHHEFFFFCC@ 0 83:T>A,84:A>C,87:T>A,88:C>A,94:T>A,95:G>A,96:A>G,97:G>A,100:T>A
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/gene_abundances.tab2 Sat Mar 31 10:17:42 2018 -0400 @@ -0,0 +1,55 @@ +gene_id transcript_id(s) length effective_length expected_count TPM FPKM +ENSG00000185097 ENST00000332831 995.00 895.00 0.00 0.00 0.00 +ENSG00000186092 ENST00000335137 918.00 818.00 0.00 0.00 0.00 +ENSG00000198744 ENST00000416718 547.00 447.00 139.00 131917.49 209967.57 +ENSG00000222623 ENST00000410691 104.00 4.00 0.00 0.00 0.00 +ENSG00000223181 ENST00000411249 104.00 4.00 0.00 0.00 0.00 +ENSG00000223659 ENST00000452176 802.00 702.00 0.00 0.00 0.00 +ENSG00000223972 ENST00000450305,ENST00000456328,ENST00000515242,ENST00000518655 1356.25 1256.25 0.00 0.00 0.00 +ENSG00000224813 ENST00000445840 180.00 80.00 0.00 0.00 0.00 +ENSG00000224956 ENST00000506640 1236.00 1136.00 4.31 1609.25 2561.38 +ENSG00000225630 ENST00000457540 1044.00 944.00 165.00 74149.30 118020.35 +ENSG00000225972 ENST00000416931 372.00 272.00 30.00 46789.40 74472.73 +ENSG00000227232 ENST00000423562,ENST00000438504,ENST00000488147,ENST00000538476,ENST00000541675 1783.00 1683.00 9.00 2268.58 3610.80 +ENSG00000228327 ENST00000428504 1317.00 1217.00 1.00 348.58 554.82 +ENSG00000228463 ENST00000335577,ENST00000424587,ENST00000442116,ENST00000448958 1925.00 1825.00 15.14 3519.31 5601.53 +ENSG00000229344 ENST00000427426 682.00 582.00 224.00 163275.16 259878.27 +ENSG00000229376 ENST00000440782 2455.00 2355.00 0.00 0.00 0.00 +ENSG00000229905 ENST00000422528 456.00 356.00 0.00 0.00 0.00 +ENSG00000230021 ENST00000414688,ENST00000419394,ENST00000440200,ENST00000441245,ENST00000447954,ENST00000448605 573.83 473.83 8.24 7376.12 11740.26 +ENSG00000230092 ENST00000447500,ENST00000590817 523.00 423.00 0.00 0.00 0.00 +ENSG00000231709 ENST00000417636 842.00 742.00 0.00 0.00 0.00 +ENSG00000233653 ENST00000432723 2477.00 2377.00 0.22 39.17 62.35 +ENSG00000233750 ENST00000442987 3812.00 3712.00 2.78 317.77 505.78 +ENSG00000235146 ENST00000423796,ENST00000450696 504.50 404.50 0.00 0.00 0.00 +ENSG00000235249 ENST00000426406 995.00 895.00 0.00 0.00 0.00 +ENSG00000235373 ENST00000416385 4860.00 4760.00 20.83 1856.35 2954.67 +ENSG00000236601 ENST00000412666,ENST00000450983 516.50 416.50 0.00 0.00 0.00 +ENSG00000236679 ENST00000458203 385.00 285.00 0.00 0.00 0.00 +ENSG00000236743 ENST00000441866 676.00 576.00 0.00 0.00 0.00 +ENSG00000237094 ENST00000419160,ENST00000423728,ENST00000425496,ENST00000431321,ENST00000431812,ENST00000432964,ENST00000440038,ENST00000440163,ENST00000453935,ENST00000455207,ENST00000455464,ENST00000599771,ENST00000601486,ENST00000601814 1108.51 1008.51 22.52 9472.96 15077.72 +ENSG00000237491 ENST00000358533,ENST00000412115,ENST00000429505,ENST00000434264,ENST00000443772,ENST00000457084,ENST00000585745,ENST00000585768,ENST00000585826,ENST00000586288,ENST00000586928,ENST00000587126,ENST00000587530,ENST00000588951,ENST00000589531,ENST00000589899,ENST00000590848,ENST00000591440,ENST00000591702,ENST00000592547,ENST00000593022 689.90 589.90 0.00 0.00 0.00 +ENSG00000237613 ENST00000417324,ENST00000461467 888.50 788.50 0.00 0.00 0.00 +ENSG00000237683 ENST00000423372 2661.00 2561.00 3.17 525.19 835.92 +ENSG00000237973 ENST00000414273 1543.00 1443.00 470.00 138174.09 219925.95 +ENSG00000238009 ENST00000453576,ENST00000466430,ENST00000471248,ENST00000477740 629.00 529.00 5.76 4620.24 7353.85 +ENSG00000239664 ENST00000357876,ENST00000440196 498.00 398.00 2.00 2131.78 3393.06 +ENSG00000239906 ENST00000493797 323.00 223.00 0.00 0.00 0.00 +ENSG00000239945 ENST00000495576 1319.00 1219.00 1.04 362.57 577.09 +ENSG00000240361 ENST00000492842 940.00 840.00 0.00 0.00 0.00 +ENSG00000240409 ENST00000467115 207.00 107.00 42.00 166517.78 265039.41 +ENSG00000240453 ENST00000435300 402.00 302.00 0.00 0.00 0.00 +ENSG00000240618 ENST00000417659 295.00 195.00 0.00 0.00 0.00 +ENSG00000241599 ENST00000496488 457.00 357.00 0.00 0.00 0.00 +ENSG00000241670 ENST00000424429,ENST00000450734 363.00 263.00 16.82 27128.05 43178.58 +ENSG00000241860 ENST00000466557,ENST00000484859,ENST00000490997,ENST00000491962 710.55 610.55 3.17 2203.21 3506.76 +ENSG00000243485 ENST00000469289,ENST00000473358,ENST00000607096 461.67 361.67 0.00 0.00 0.00 +ENSG00000248527 ENST00000514057 681.00 581.00 295.00 215397.66 342839.48 +ENSG00000250575 ENST00000514436 1239.00 1139.00 0.00 0.00 0.00 +ENSG00000256186 ENST00000540477 90.00 0.00 0.00 0.00 0.00 +ENSG00000268020 ENST00000594647,ENST00000606857 483.00 383.00 0.00 0.00 0.00 +ENSG00000268663 ENST00000438434 424.00 324.00 0.00 0.00 0.00 +ENSG00000268903 ENST00000494149 755.00 655.00 0.00 0.00 0.00 +ENSG00000269732 ENST00000437905 363.00 263.00 0.00 0.00 0.00 +ENSG00000269831 ENST00000599533 129.00 29.00 0.00 0.00 0.00 +ENSG00000269981 ENST00000595919 284.00 184.00 0.00 0.00 0.00
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/isoform_abundances.tab2 Sat Mar 31 10:17:42 2018 -0400 @@ -0,0 +1,118 @@ +transcript_id gene_id length effective_length expected_count TPM FPKM IsoPct +ENST00000332831 ENSG00000185097 995 895.00 0.00 0.00 0.00 0.00 +ENST00000335137 ENSG00000186092 918 818.00 0.00 0.00 0.00 0.00 +ENST00000416718 ENSG00000198744 547 447.00 139.00 131917.49 209967.57 100.00 +ENST00000410691 ENSG00000222623 104 4.00 0.00 0.00 0.00 0.00 +ENST00000411249 ENSG00000223181 104 4.00 0.00 0.00 0.00 0.00 +ENST00000452176 ENSG00000223659 802 702.00 0.00 0.00 0.00 0.00 +ENST00000450305 ENSG00000223972 632 532.00 0.00 0.00 0.00 0.00 +ENST00000456328 ENSG00000223972 1657 1557.00 0.00 0.00 0.00 0.00 +ENST00000515242 ENSG00000223972 1653 1553.00 0.00 0.00 0.00 0.00 +ENST00000518655 ENSG00000223972 1483 1383.00 0.00 0.00 0.00 0.00 +ENST00000445840 ENSG00000224813 180 80.00 0.00 0.00 0.00 0.00 +ENST00000506640 ENSG00000224956 1236 1136.00 4.31 1609.25 2561.38 100.00 +ENST00000457540 ENSG00000225630 1044 944.00 165.00 74149.30 118020.35 100.00 +ENST00000416931 ENSG00000225972 372 272.00 30.00 46789.40 74472.73 100.00 +ENST00000423562 ENSG00000227232 1669 1569.00 0.00 0.00 0.00 0.00 +ENST00000438504 ENSG00000227232 1783 1683.00 9.00 2268.58 3610.80 100.00 +ENST00000488147 ENSG00000227232 1351 1251.00 0.00 0.00 0.00 0.00 +ENST00000538476 ENSG00000227232 1583 1483.00 0.00 0.00 0.00 0.00 +ENST00000541675 ENSG00000227232 1416 1316.00 0.00 0.00 0.00 0.00 +ENST00000428504 ENSG00000228327 1317 1217.00 1.00 348.58 554.82 100.00 +ENST00000335577 ENSG00000228463 510 410.00 0.00 0.00 0.00 0.00 +ENST00000424587 ENSG00000228463 2257 2157.00 0.00 0.00 0.00 0.00 +ENST00000442116 ENSG00000228463 1292 1192.00 0.00 0.00 0.00 0.00 +ENST00000448958 ENSG00000228463 1925 1825.00 15.14 3519.31 5601.53 100.00 +ENST00000427426 ENSG00000229344 682 582.00 224.00 163275.16 259878.27 100.00 +ENST00000440782 ENSG00000229376 2455 2355.00 0.00 0.00 0.00 0.00 +ENST00000422528 ENSG00000229905 456 356.00 0.00 0.00 0.00 0.00 +ENST00000414688 ENSG00000230021 750 650.00 0.00 0.00 0.00 0.00 +ENST00000419394 ENSG00000230021 480 380.00 0.00 0.00 0.00 0.00 +ENST00000440200 ENSG00000230021 413 313.00 0.00 0.00 0.00 0.00 +ENST00000441245 ENSG00000230021 629 529.00 7.24 5804.92 9239.45 78.70 +ENST00000447954 ENSG00000230021 336 236.00 0.00 0.00 0.00 0.00 +ENST00000448605 ENSG00000230021 370 270.00 1.00 1571.20 2500.81 21.30 +ENST00000447500 ENSG00000230092 523 423.00 0.00 0.00 0.00 0.00 +ENST00000590817 ENSG00000230092 523 423.00 0.00 0.00 0.00 0.00 +ENST00000417636 ENSG00000231709 842 742.00 0.00 0.00 0.00 0.00 +ENST00000432723 ENSG00000233653 2477 2377.00 0.22 39.17 62.35 100.00 +ENST00000442987 ENSG00000233750 3812 3712.00 2.78 317.77 505.78 100.00 +ENST00000423796 ENSG00000235146 607 507.00 0.00 0.00 0.00 0.00 +ENST00000450696 ENSG00000235146 402 302.00 0.00 0.00 0.00 0.00 +ENST00000426406 ENSG00000235249 995 895.00 0.00 0.00 0.00 0.00 +ENST00000416385 ENSG00000235373 4860 4760.00 20.83 1856.35 2954.67 100.00 +ENST00000412666 ENSG00000236601 426 326.00 0.00 0.00 0.00 0.00 +ENST00000450983 ENSG00000236601 607 507.00 0.00 0.00 0.00 0.00 +ENST00000458203 ENSG00000236679 385 285.00 0.00 0.00 0.00 0.00 +ENST00000441866 ENSG00000236743 676 576.00 0.00 0.00 0.00 0.00 +ENST00000419160 ENSG00000237094 607 507.00 0.00 0.00 0.00 0.00 +ENST00000423728 ENSG00000237094 573 473.00 2.00 1793.56 2854.73 18.93 +ENST00000425496 ENSG00000237094 2661 2561.00 14.52 2405.24 3828.32 25.39 +ENST00000431321 ENSG00000237094 406 306.00 0.00 0.00 0.00 0.00 +ENST00000431812 ENSG00000237094 336 236.00 0.00 0.00 0.00 0.00 +ENST00000432964 ENSG00000237094 575 475.00 0.00 0.20 0.32 0.00 +ENST00000440038 ENSG00000237094 746 646.00 3.03 1990.08 3167.53 21.01 +ENST00000440163 ENSG00000237094 462 362.00 0.00 0.00 0.00 0.00 +ENST00000453935 ENSG00000237094 498 398.00 2.00 2131.78 3393.06 22.50 +ENST00000455207 ENSG00000237094 413 313.00 0.00 0.00 0.00 0.00 +ENST00000455464 ENSG00000237094 735 635.00 0.00 0.00 0.00 0.00 +ENST00000599771 ENSG00000237094 457 357.00 0.97 1152.11 1833.76 12.16 +ENST00000601486 ENSG00000237094 696 596.00 0.00 0.01 0.01 0.00 +ENST00000601814 ENSG00000237094 635 535.00 0.00 0.00 0.00 0.00 +ENST00000358533 ENSG00000237491 1194 1094.00 0.00 0.00 0.00 0.00 +ENST00000412115 ENSG00000237491 581 481.00 0.00 0.00 0.00 0.00 +ENST00000429505 ENSG00000237491 441 341.00 0.00 0.00 0.00 0.00 +ENST00000434264 ENSG00000237491 513 413.00 0.00 0.00 0.00 0.00 +ENST00000443772 ENSG00000237491 417 317.00 0.00 0.00 0.00 0.00 +ENST00000457084 ENSG00000237491 566 466.00 0.00 0.00 0.00 0.00 +ENST00000585745 ENSG00000237491 168 68.00 0.00 0.00 0.00 0.00 +ENST00000585768 ENSG00000237491 394 294.00 0.00 0.00 0.00 0.00 +ENST00000585826 ENSG00000237491 390 290.00 0.00 0.00 0.00 0.00 +ENST00000586288 ENSG00000237491 1040 940.00 0.00 0.00 0.00 0.00 +ENST00000586928 ENSG00000237491 598 498.00 0.00 0.00 0.00 0.00 +ENST00000587126 ENSG00000237491 495 395.00 0.00 0.00 0.00 0.00 +ENST00000587530 ENSG00000237491 881 781.00 0.00 0.00 0.00 0.00 +ENST00000588951 ENSG00000237491 607 507.00 0.00 0.00 0.00 0.00 +ENST00000589531 ENSG00000237491 714 614.00 0.00 0.00 0.00 0.00 +ENST00000589899 ENSG00000237491 809 709.00 0.00 0.00 0.00 0.00 +ENST00000590848 ENSG00000237491 726 626.00 0.00 0.00 0.00 0.00 +ENST00000591440 ENSG00000237491 780 680.00 0.00 0.00 0.00 0.00 +ENST00000591702 ENSG00000237491 1873 1773.00 0.00 0.00 0.00 0.00 +ENST00000592547 ENSG00000237491 608 508.00 0.00 0.00 0.00 0.00 +ENST00000593022 ENSG00000237491 693 593.00 0.00 0.00 0.00 0.00 +ENST00000417324 ENSG00000237613 1187 1087.00 0.00 0.00 0.00 0.00 +ENST00000461467 ENSG00000237613 590 490.00 0.00 0.00 0.00 0.00 +ENST00000423372 ENSG00000237683 2661 2561.00 3.17 525.19 835.92 100.00 +ENST00000414273 ENSG00000237973 1543 1443.00 470.00 138174.09 219925.95 100.00 +ENST00000453576 ENSG00000238009 336 236.00 0.00 0.00 0.00 0.00 +ENST00000466430 ENSG00000238009 2748 2648.00 0.00 0.00 0.00 0.00 +ENST00000471248 ENSG00000238009 629 529.00 5.76 4620.24 7353.85 100.00 +ENST00000477740 ENSG00000238009 491 391.00 0.00 0.00 0.00 0.00 +ENST00000357876 ENSG00000239664 498 398.00 2.00 2131.78 3393.06 100.00 +ENST00000440196 ENSG00000239664 439 339.00 0.00 0.00 0.00 0.00 +ENST00000493797 ENSG00000239906 323 223.00 0.00 0.00 0.00 0.00 +ENST00000495576 ENSG00000239945 1319 1219.00 1.04 362.57 577.09 100.00 +ENST00000492842 ENSG00000240361 940 840.00 0.00 0.00 0.00 0.00 +ENST00000467115 ENSG00000240409 207 107.00 42.00 166517.78 265039.41 100.00 +ENST00000435300 ENSG00000240453 402 302.00 0.00 0.00 0.00 0.00 +ENST00000417659 ENSG00000240618 295 195.00 0.00 0.00 0.00 0.00 +ENST00000496488 ENSG00000241599 457 357.00 0.00 0.00 0.00 0.00 +ENST00000424429 ENSG00000241670 363 263.00 16.82 27128.05 43178.58 100.00 +ENST00000450734 ENSG00000241670 457 357.00 0.00 0.00 0.00 0.00 +ENST00000466557 ENSG00000241860 1301 1201.00 1.53 541.79 862.35 24.59 +ENST00000484859 ENSG00000241860 4860 4760.00 0.00 0.00 0.00 0.00 +ENST00000490997 ENSG00000241860 518 418.00 1.64 1661.42 2644.41 75.41 +ENST00000491962 ENSG00000241860 278 178.00 0.00 0.00 0.00 0.00 +ENST00000469289 ENSG00000243485 535 435.00 0.00 0.00 0.00 0.00 +ENST00000473358 ENSG00000243485 712 612.00 0.00 0.00 0.00 0.00 +ENST00000607096 ENSG00000243485 138 38.00 0.00 0.00 0.00 0.00 +ENST00000514057 ENSG00000248527 681 581.00 295.00 215397.66 342839.48 100.00 +ENST00000514436 ENSG00000250575 1239 1139.00 0.00 0.00 0.00 0.00 +ENST00000540477 ENSG00000256186 90 0.00 0.00 0.00 0.00 0.00 +ENST00000594647 ENSG00000268020 126 26.00 0.00 0.00 0.00 0.00 +ENST00000606857 ENSG00000268020 840 740.00 0.00 0.00 0.00 0.00 +ENST00000438434 ENSG00000268663 424 324.00 0.00 0.00 0.00 0.00 +ENST00000494149 ENSG00000268903 755 655.00 0.00 0.00 0.00 0.00 +ENST00000437905 ENSG00000269732 363 263.00 0.00 0.00 0.00 0.00 +ENST00000599533 ENSG00000269831 129 29.00 0.00 0.00 0.00 0.00 +ENST00000595919 ENSG00000269981 284 184.00 0.00 0.00 0.00 0.00