view bamsort.xml @ 0:7da2c9654a83 draft default tip

Uploaded

<tool id="bamsort" name="Sort BAM file">
  <description>Sort a BAM file by coordinates (or names) of the mapped reads</description>
  <requirements>
    <requirement type="package" version="3.4.1">python3</requirement>
    <requirement type="package" version="0.1.3_9af04e0e9125">MiModD</requirement>
</requirements>
  <command>
	mimodd sort $inputfile -o $output --oformat $oformat $by_name
  </command>
  
  <inputs>
    <param name="inputfile" type="data" format="bam" label="Input file to sort" />
    <param name="by_name" type="boolean" truevalue = "-n" falsevalue ="" label="Sort by read names instead of coordinates" checked = "false" help="A less common option, but necessary, e.g., if you want to re-align sorted output from a previous run of the Snap Align Tool." />
    <param name="oformat" type="boolean" truevalue = "sam" falsevalue = "bam" label = "Output in uncompressed SAM format" checked = "false" />
  </inputs>
  
  <outputs>
    <data name="output" format="bam" label="Sorted output from MiModd ${tool.name} on ${on_string}">
      <change_format>
	 <when input="oformat" value="sam" format="sam" />
      </change_format>
    </data>
  </outputs>

<help>
.. class:: infomark

   **What it does**

The tool sorts a BAM file of aligned reads, typically by the reference genome coordinates that the reads have been mapped to.

Coordinate-sorted input files are expected by the downstream MiModD tools *Variant Calling and Coverage Analysis* and *Deletion prediction*.

Note, however, that the *SNAP Read Alignment* produces coordinate-sorted output by default and it is only necessary to sort files that come from other sources or from *SNAP Read Alignment* jobs with a custom sort order.

The option *Sort by read names instead of coordinates* is useful if you want to re-align coordinate-sorted paired-end data. In *paired-end mode*, the *SNAP Read Alignment* tool expects the reads in the input file to be arranged in read pairs, i.e., the forward read information of a pair must be followed immediately by its reverse mate information, which is typically not the case in coordinate-sorted files. Resorting such files by read names fixes this problem.

</help>
</tool>