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1 <tool id="bamsort" name="Sort BAM file">
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2 <description>Sort a BAM file by coordinates (or names) of the mapped reads</description>
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3 <requirements>
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4 <requirement type="package" version="3.4.1">python3</requirement>
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5 <requirement type="package" version="0.1.3_9af04e0e9125">MiModD</requirement>
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6 </requirements>
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7 <command>
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8 mimodd sort $inputfile -o $output --oformat $oformat $by_name
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9 </command>
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10
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11 <inputs>
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12 <param name="inputfile" type="data" format="bam" label="Input file to sort" />
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13 <param name="by_name" type="boolean" truevalue = "-n" falsevalue ="" label="Sort by read names instead of coordinates" checked = "false" help="A less common option, but necessary, e.g., if you want to re-align sorted output from a previous run of the Snap Align Tool." />
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14 <param name="oformat" type="boolean" truevalue = "sam" falsevalue = "bam" label = "Output in uncompressed SAM format" checked = "false" />
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15 </inputs>
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16
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17 <outputs>
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18 <data name="output" format="bam" label="Sorted output from MiModd ${tool.name} on ${on_string}">
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19 <change_format>
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20 <when input="oformat" value="sam" format="sam" />
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21 </change_format>
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22 </data>
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23 </outputs>
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24
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25 <help>
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26 .. class:: infomark
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27
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28 **What it does**
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29
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30 The tool sorts a BAM file of aligned reads, typically by the reference genome coordinates that the reads have been mapped to.
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31
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32 Coordinate-sorted input files are expected by the downstream MiModD tools *Variant Calling and Coverage Analysis* and *Deletion prediction*.
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33
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34 Note, however, that the *SNAP Read Alignment* produces coordinate-sorted output by default and it is only necessary to sort files that come from other sources or from *SNAP Read Alignment* jobs with a custom sort order.
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35
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36 The option *Sort by read names instead of coordinates* is useful if you want to re-align coordinate-sorted paired-end data. In *paired-end mode*, the *SNAP Read Alignment* tool expects the reads in the input file to be arranged in read pairs, i.e., the forward read information of a pair must be followed immediately by its reverse mate information, which is typically not the case in coordinate-sorted files. Resorting such files by read names fixes this problem.
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37
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38 </help>
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39 </tool>
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40
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