view ctat_gmap_fusion.xml @ 3:d4f7fcb63dad draft default tip

Updating files related to lncrna data manager.

<tool id="ctat_gmap_fusion" name="ctat_gmap_fusion" version="1.0.0" profile="17.05">
    <description>Fusion-finding Using De novo RNA-Seq Transcript Assemblies</description>
    <requirements>
        <requirement type="package" version="0.4.0">gmap-fusion</requirement>
    </requirements>
    <command detect_errors="default">
      GMAP-fusion 
      -T $assembled_trans 
      --left_fq $left_input
      --right_fq $right_input
      --genome_lib_dir "${genome_resource_lib.fields.path}"
      --output "subdir" 
    </command>
    <stdio>
      <exit_code range="1:"  level="fatal"   description="Error returned from pipeline" />
    </stdio>
    <regex match="Must investigate error above."
           source="stderr"
           level="fatal"
           description="Unknown error encountered" />
    <inputs>
      <param format="fasta" name="assembled_trans" type="data" label="Assembled Transcripts (ie. Trinity or DISCASM output)" help="Trinity or DISCASM generated transcript fasta"/>
      <param format="fastq" name="left_input" type="data" label="Left/Forward strand reads" help="Forward strand reads"/>
      <param format="fastq" name="right_input" type="data" label="Right/Reverse strand reads" help="Reverse strand reads"/>
      <param name="genome_resource_lib" type="select" label="Select a reference genome">
        <options from_data_table="ctat_genome_resource_libs">
          <filter type="sort_by" column="2" />
          <validator type="no_options" message="No indexes are available" />
        </options>
      </param>
    </inputs>
    <outputs>
      <data format="tabular" name="gmapfusion_candidates" label="${tool.name} on ${on_string}: GMAP-fusion candidates" from_work_dir="subdir/GMAP-fusion.final"/>
    </outputs>

    <tests>
      <test>
        <param name="left_input" value="GMAP/reads_1.fq.gz" />
        <param name="right_input" value="GMAP/reads_2.fq.gz" />
        <param name="assembled_trans" value="GMAP/transcripts.fa" />
        <!-- FIX - now that we added the CTAT ref lib path as a parameter, how do we find it for testing?
        <param name="genome_resource_lib.fields.path" value="?????" />
        -->
        <output name="gmapfusion_candidates" file="GMAP/fusion.reads_1_2.final" />
      </test>
    </tests>

    <help>
.. class:: infomark

GMAP-fusion is a utility for identifying candidate fusion transcripts based on transcript sequences reconstructed via RNA-Seq de novo transcriptome assembly.  Please read more here_.

.. _here: https://github.com/GMAP-fusion/GMAP-fusion/wiki
    </help>
</tool>