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1 <tool id="ctat_genome_resource_libs_data_manager"
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2 name="CTAT Genome Resource Libraries Data Manager"
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3 version="2.0.0" tool_type="manage_data">
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4 <!-- This Data Manager tool was written by Cicada Dennis of Indiana University for the Broad Institute.
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5 -->
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6 <description>Retrieve, and/or specify the location of, a CTAT Genome Resource Library.
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7 </description>
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8 <requirements>
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9 <requirement type="package" version="2.7">python</requirement>
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10 <requirement type="package" version="0.5.0">fusion-filter</requirement>
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11 <requirement type="package" version="2.0.1">ctat-mutations</requirement>
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12 </requirements>
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13 <command detect_errors="exit_code">
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14 <![CDATA[
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15 python $__tool_directory__/add_ctat_resource_lib.py
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16 --output_filename="${out_file}"
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17 --display_name="${display_name}"
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18 --cravat_tissues_filepath="${__tool_directory__}/../tool-data/ctat_cravat_tissues.loc.sample"
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19 #if str( $genome_resource_library.build_type ) == "download_and_build":
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20 --download_url="${genome_resource_library.download_url}"
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21 --download_location="${genome_resource_library.download_destination}"
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22 #if str( $genome_resource_library.force_new_download ) == "true":
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23 --new_archive_download
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24 #end if
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25 #if str( $genome_resource_library.keep_archive ) == "true":
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26 --keep_archive
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27 #end if
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28 #if str( $genome_resource_library.rebuild ) == "true":
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29 --new_library_build
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30 #end if
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31 #if str( $genome_resource_library.specify_build_location.build_location ) == "true":
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32 --build_location="${genome_resource_library.specify_build_location.different_build_location}"
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33 #end if
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34 #elif str( $genome_resource_library.build_type ) == "build_from_source":
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35 --source_location "${genome_resource_library.source_location}"
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36 #if str( $genome_resource_library.rebuild ) == "true":
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37 --new_library_build
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38 #end if
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39 #if str( $genome_resource_library.specify_build_location.build_location ) == "true":
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40 --build_location="${genome_resource_library.specify_build_location.different_build_location}"
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41 #end if
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42 #elif str( $genome_resource_library.build_type ) == "specify_built_location":
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43 --build_location="${genome_resource_library.built_library_location}"
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44 #end if
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45
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46 #if str( $gmap_options.gmap_build ) == "true":
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47 --gmap_build
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48 #if str( $gmap_options.force_gmap_build ) == "true":
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49 --force_gmap_build
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50 #end if
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51 #end if
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52
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53 #if str( $mutation_lib_options.mutation_build ) == "true":
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54 --download_mutation_resources_url="${mutation_lib_options.source_url}"
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55 --cosmic_resources_location="${mutation_lib_options.cosmic_files_location}"
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56 #if str( $mutation_lib_options.force_download ) == "true":
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57 --new_mutation_download
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58 #end if
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59 #if str( $mutation_lib_options.redo_integration ) == "true":
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60 --new_mutation_integration
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61 #end if
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62 #end if
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63 ]]>
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64 </command>
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65 <inputs>
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66 <conditional name="genome_resource_library">
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67 <param name="build_type" type="select" label="Download CTAT Genome Resource Library?">
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68 <option value="download_and_build" selected="true">Download from CTAT and build if needed</option>
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69 <option value="build_from_source">Build library from local source data</option>
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70 <option value="specify_built_location">Specify location of built library</option>
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71 </param>
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72 <when value="download_and_build">
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73 <!-- The use of a code block to get dynamic options is now deprecated and discouraged.
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74 I am still using it here. The only other way I can think of to do this is to
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75 create another data_manager that gets the list of files and puts them into a
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76 data_table, that is then used to get the filenames. That would require the admin
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77 to first run the data_manager that builds the filename data_table before running
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78 this data_manager.
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79 This is the dynamic way to get the options filled.
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80 <param name="filename" type="select" label="Select File" display="radio"
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81 dynamic_options="get_ctat_genome_urls()"
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82 help="Select a CTAT Genome Resource Library to Download." />
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83 Here is the static method for what is online in April 2017:
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84 <param name="filename" type="select" label="Choose which library to download.">
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85 <option value="https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/GRCh37_v19_CTAT_lib_Feb092018.plug-n-play.tar.gz">
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86 GRCh37_v19_CTAT_lib_Feb092018.plug-n-play.tar.gz
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87 </option>
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88 <option value="https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/ GRCh37_v19_CTAT_lib_Feb092018.source_data.tar.gz">
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89 GRCh37_v19_CTAT_lib_Feb092018.source_data.tar.gz
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90 </option>
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91 <option value="https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/ GRCh38_v27_CTAT_lib_Feb092018.plug-n-play.tar.gz">
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92 GRCh38_v27_CTAT_lib_Feb092018.plug-n-play.tar.gz
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93 </option>
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94 <option value="https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/ GRCh38_v27_CTAT_lib_Feb092018.source_data.tar.gz">
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95 GRCh38_v27_CTAT_lib_Feb092018.source_data.tar.gz
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96 </option>
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97 <option value="https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/ Mouse_M16_CTAT_lib_Feb202018.plug-n-play.tar.gz">
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98 Mouse_M16_CTAT_lib_Feb202018.plug-n-play.tar.gz
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99 </option>
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100 <option value="https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/ Mouse_M16_CTAT_lib_Feb202018.source_data.tar.gz">
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101 Mouse_M16_CTAT_lib_Feb202018.source_data.tar.gz
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102 </option>
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103 -->
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104 <param name="download_url" type="select" label="Select a File"
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105 dynamic_options="get_ctat_genome_urls()"
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106 help="Select a CTAT Genome Resource Library to Download.">
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107 </param>
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108 <param name="download_destination" type="text" label="Download Destination (full path)" />
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109 <param name="force_new_download" type="boolean" checked="false" label="Force New Download?" />
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110 <param name="keep_archive" type="boolean" checked="true" label="Keep downloaded archive rather than deleting after build?" />
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111 <param name="rebuild" type="boolean" checked="false" label="Force new build of Library?" />
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112 <conditional name="specify_build_location">
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113 <param name="build_location" type="boolean" checked="false" label="Is the build location different than the source location?" />
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114 <when value="true">
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115 <param name="different_build_location" type="text" label="Build Location (full path)" />
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116 </when>
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117 </conditional>
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118 </when>
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119 <when value="build_from_source">
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120 <param name="source_location" type="text" label="Location of Source Files (full path)" />
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121 <param name="rebuild" type="boolean" checked="false" label="Force new build of Library?" />
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122 <conditional name="specify_build_location">
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123 <param name="build_location" type="boolean" checked="false" label="Is the build location different than the source location?" />
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124 <when value="true">
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125 <param name="different_build_location" type="text" label="Build Location (full path)" />
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126 </when>
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127 </conditional>
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128 </when>
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129 <when value="specify_built_location">
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130 <param name="built_library_location" type="text" label="Location of the Built Library (full path)" />
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131 </when>
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132 </conditional>
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133 <param name="display_name" type="text" label="Reference Genome Display Name" />
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134 <conditional name="gmap_options">
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135 <param name="gmap_build" type="boolean" checked="true" label="Do a gmap_build on the Library?" />
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136 <when value="true">
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137 <param name="force_gmap_build" type="boolean" checked="false" label="Force a new build even if previously done?" />
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138 </when>
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139 </conditional>
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140 <conditional name="mutation_lib_options">
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141 <param name="mutation_build" type="boolean" checked="false" label="Download and integrate mutation library?">
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142 </param>
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143 <when value="true">
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144 <param name="source_url" type="select" label="Select a File"
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145 dynamic_options="get_mutation_resource_urls()"
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146 help="Select CTAT Mutation Library File to Download.\nMake sure it is the right one for your CTAT Genome Resource Library!">
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147 </param>
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148 <param name="force_download" type="boolean" checked="false" label="Force New Download?" />
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149 <param name="redo_integration" type="boolean" checked="false" label="Redo Library Integration?" />
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150 <param name="cosmic_files_location" type="text" label="Location of the COSMIC files (See Tool Notes)." />
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151 </when>
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152 </conditional>
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153 </inputs>
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154 <outputs>
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155 <data name="out_file" format="data_manager_json" />
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156 </outputs>
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157 <help>
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158 Retrieve, and/or specify the location of, a CTAT Genome Resource Library.
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159
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160 There are three options: 1) Download from CTAT and build if needed; 2) Build library from local source data; 3) Specify location of built library.
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161
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162 1) Download from CTAT and build if needed:
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163 This option uses the files at https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/
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164 as selectors for the user to choose among.
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165 Specify the Full Path of the location where the CTAT Genome Resource Library should be placed.
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166 The other options should be self-explanatory.
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167 2) Build library from local source data:
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168 This assumes that you have already downloaded the source data for a build and you want to build it.
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169 3) Specify location of built library:
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170 This assumes you already have a build library and need tell galaxy where it is.
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171
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172 All options allow the user to do a gmap_build on the library
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173 and also to integrate ctat-mutation resources into the library.
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174
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175 You will need approximately 62GB of space for a human genome resource library, once it is built,
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176 but if downloading and building, to be safe provide at least 75GB.
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177
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178 The installation of this tool takes some time, due to building a conda environment for the dependencies.
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179 The "source_data" files download faster, but then must be built.
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180 Building the library from the "source_data" files can take many hours, depending on the resources of your machine.
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181 The "plug-n-play" can take considerable time to download, depending on your internet connection. Even with high speed,
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182 it is about 25GB that is transfered, so plan accordingly.
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183 If you have a good speed internet connection, downloading the plug-n-play will usually be faster than building.
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184
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185 **If a download or a build is interrupted, re-running the job should pick up where it left off.**
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186
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187 Neither the "source_data" nor the "plug-n-play" versions have had their gmap index built. If you are not going to be
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188 using gmap_fusion, then you can uncheck the gmap_build check box and save the space and time building the index consumes.
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189
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190 Neither the "source_data" nor the "plug-n-play" versions have Mutation Resources included.
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191 Those must be downloaded separately and integrated into the Library. If you are going to be using the
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192 ctat_mutations tool, check the Download Mutation Library check box. Whether or not you check this box, the
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193 ctat_cravat_tissues table needed by the ctat_mutations tool will be created.
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194
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195 In order to integrate the Mutation Resources into a CTAT Genome Resource Library, you must have previously downloaded
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196 COSMIC resources (See Step 2 from https://github.com/NCIP/ctat-mutations/tree/master/mutation_lib_prep )
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197 You can place them directly into the Genome Resource Library location, or if the Library is
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198 not built yet, or you do not know the full path to it, specify the directory where the COSMIC files are, so they can be
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199 integrated into the Library.
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200
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201 The Mouse genome is not currently supported by ctat_mutations.
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202
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203 If the Reference Genome Display Name is left empty a name will be created,
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204 but any text that will best guide the user can be entered here.
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205 It will be the text that is used for selecting the library in pull down lists
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206 requiring a Genome Reference Library resource (These are stored in the ctat_genome_resource_libs table).
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207
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208 For more information on CTAT Genome Resource Libraries,
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209 see https://github.com/FusionFilter/FusionFilter/wiki
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210 </help>
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211 <code file="add_ctat_resource_lib.py" />
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212 </tool>
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