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1 FALCO Amplicon Analysis Pipeline & Galaxy wrapper
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2
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2 ========================================
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3
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4 FALCO variant-caller is part of the Amplicon Analysis Pipeline (AAP).
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5
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6 The typical workflow is as follows:
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7 * paired-end amplicon sequencing
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8 * merge pairs by overlapping them
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9 * map the single read fastq with BWA
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10 * perform variant calling with FALCO
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11 * create (html) report of the results
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12
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13 FALCO uses samtools and straight-forward statistics to determine wether a
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14 potential variant is likely a (technical) artifact or not.
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15
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16 Input / Output
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17 =======
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18
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19 Input of the caller is a BAM file, output VCF
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2
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20
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21
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22 History
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23 =======
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24
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25 ============== ================================================================
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26 Date Changes
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27 -------------- ----------------------------------------------------------------
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28 December 2014 * first release in Test Tool Shed
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29 ============== ================================================================
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30
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31
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32 Bug Reports & other questions
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33 =============================
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34
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35 Issues can be reported via http://www.tgac.nl/
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