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annotate AffyQCnormalization.xml @ 15:c05f899d5dcd draft
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author | sblanck |
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date | Tue, 16 May 2017 02:08:10 -0400 |
parents | 328f4031e5d3 |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit da1436252b5db4e90c39c95140558f0f93544160-dirty
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1 <tool id="QCnormalization" name="QCnormalization" version="1.0.0"> |
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2 |
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3 <description>Quality control and normalization of affymetrix expression data</description> |
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4 |
8 | 5 <requirements> |
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6 <requirement type="package" version="0.1.0">r-smagexp</requirement> |
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7 </requirements> |
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8 |
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9 <stdio> |
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10 <exit_code range="1:" /> |
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11 <regex match="Warning" source="both" level="warning"/> |
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12 </stdio> |
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13 |
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14 <command> |
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15 <![CDATA[ |
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16 Rscript |
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17 ${__tool_directory__}/AffyQCnormalization.R |
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18 --input "#for $input in $inputs# $input;$input.name, #end for#" |
8 | 19 --normalization ${normalization} |
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20 --rdataoutput $result_export_eset |
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21 --htmloutput $result_html |
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22 --htmloutputpath $result_html.files_path |
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23 --htmltemplate ${__tool_directory__}/AffyQCnormalization_tpl.html |
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24 ]]> |
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25 </command> |
2 | 26 |
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27 <inputs> |
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28 <param name="inputs" type="data" format="cel" multiple="true" label=".CEL files" help=".CEL files to be used"/> |
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29 <param name="normalization" type="select" label="Preprocessing/normalization"> |
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30 <option value="rma">rma (backgroung correction + quantile normalization + log2)</option> |
2 | 31 <option value="quantile">quantile normalization + log2</option> |
32 <option value="background">background correction + log2</option> | |
33 <option value="log2">log2 only</option> | |
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34 </param> |
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35 </inputs> |
2 | 36 |
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37 <outputs> |
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38 <data format="rdata" name="result_export_eset" label="export normalized expressionSet"/> |
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39 <data format="html" name="result_html" label="QC result"/> |
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40 </outputs> |
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41 |
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42 <help> |
2 | 43 <![CDATA[ |
44 **What it does** | |
45 | |
46 The QCnormalization tool offers to ensure the quality of the data and to normalize them. Several normalization methods are available : | |
47 | |
48 * rma normalization | |
49 * quantile normalization + log2 | |
50 * background correction + log2 | |
51 * log2 only | |
52 | |
53 **Results** | |
54 | |
55 - Several quality figures : microarray images, boxplots and MA plots | |
56 - Rdata object containing the normalized data for further analysis | |
57 | |
58 ]]> | |
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59 </help> |
2 | 60 |
61 </tool> |