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2
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1 <tool id="GEOQuery" name="GEOQuery">
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2 <description>GEOQuery wrapper</description>
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3
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3 <stdio>
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4 <exit_code range="1:" />
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5 </stdio>
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8
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6 <!--requirements>
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7 <!container type="docker">sblanck/smagexp</container>
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2
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8 <requirement type="package" version="0.1.0">r-smagexp</requirement>
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8
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9 </requirements-->
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3
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10 <command>
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2
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11 <![CDATA[
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12 Rscript --vanilla
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13 ${__tool_directory__}/GEOQuery.R
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14 --id ${GEOQueryID}
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15 --transformation ${transformation}
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16 --data ${GEOQueryData}
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17 --rdata ${GEOQueryRData}
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18 --conditions ${conditions}
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2
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19 ]]>
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20 </command>
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21
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22 <inputs>
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23 <param name="GEOQueryID" type="text" size="12" optional="false" label="GEOQuery ID" help="">
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24 <validator type="empty_field"/>
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25 </param>
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26 <param name="transformation" type="select" label="log2 transformation">
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27 <option value="auto">auto</option>
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28 <option value="yes">yes</option>
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29 <option value="no">no</option>
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30 </param>
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31 </inputs>
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32
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33 <outputs>
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34 <data format="tabular" name="GEOQueryData" label="GEOQuery Data of ${GEOQueryID}"/>
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35 <data format="cond" name="conditions" label="conditions of ${GEOQueryID}"/>
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36 <data format="rdata" name="GEOQueryRData" label="GEOQuery RData of ${GEOQueryID}"/>
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37 </outputs>
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38 <help>
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39 <![CDATA[
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40 **What it does**
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41
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42 This tool fetches microarray data directly from GEO database, based on the GEOQuery R package. Given a GSE accession ID, it returns an Rdata object containing the data and a text file (.cond file) summarizing the conditions of the experiment.
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43 The .cond file is a text file containing one line per sample in the experiment. Each line is made of 3 columns:
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44
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45 - Sample ID
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46 - Condition of the biological sample
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47 - Description of the biological sample
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48
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49 **Example** of .cond file
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50 ::
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51
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52 GSM80460 series of 16 tumors GSM80460 OSCE-2T SERIES OF 16 TUMORS
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53 GSM80461 series of 16 tumors GSM80461 OSCE-4T Series of 16 Tumors
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54 GSM80462 series of 16 tumors GSM80462 OSCE-6T Series of 16 Tumors
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55 GSM80476 series of 4 normals GSM80476 OSCE-2N Series of 4 Normals
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56 GSM80477 series of 4 normals GSM80477 OSCE-9N Series of 4 Normals
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57
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58 **Results**
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59
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60 - Rdata object containing data for further analysis
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61 - Condition (.cond) file summarizing conditions of the experiment
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62 - Tabular (.txt) file containing expression data for each sample
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63 ]]>
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64
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65 </help>
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66
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67
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68 </tool>
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