Mercurial > repos > sanbi-uwc > novo_align
changeset 6:3938f90c9d91 draft
planemo upload for repository https://github.com/zipho/novo_align commit 4a89d4d866533a93706ceb29077d2e9bda69aa9f
author | sanbi-uwc |
---|---|
date | Fri, 11 Mar 2016 02:32:25 -0500 |
parents | d51c5af7a8fe |
children | b91ad2488816 |
files | novo_align.py |
diffstat | 1 files changed, 4 insertions(+), 14 deletions(-) [+] |
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--- a/novo_align.py Fri Mar 11 02:02:09 2016 -0500 +++ b/novo_align.py Fri Mar 11 02:32:25 2016 -0500 @@ -1,18 +1,12 @@ #!/usr/bin/env python - from __future__ import print_function import argparse -from subprocess import check_call, CalledProcessError, Popen import shlex import os import logging log = logging.getLogger( __name__ ) def novo_align(output_filename, index_filename, fwd_file, rev_file ): - #novoalign -c 8 -k -d /cip0/research/ajayi/RNA-seq_Analysis_Project_Case_Study/reference/Homo_Sapiens/out/TB_H37Rv.nix - # -f X165_820L8_.R1_val_1.fq X165_820L8_.R2_val_2.fq -i PE 250,100 - # -o SAM '@RG\tID:readgroup\tPU:platform unit\tLB:library' | samtools view -bS - > `pwd`/out/X165_820L8.bam - #output_filename = path.join(output_directory, fwd_file.split(".")[0] + ".bam") param = r'@RG\tID:RG\tSM:$i\tPL:ILLUMINA' cmdline_str = "novoalign -c 8 -k -d {} -f {} {} -i PE 250, 100 -o SAM '{}' | samtools view -bS - > {}".format( index_filename, @@ -20,13 +14,10 @@ rev_file, param, output_filename) - #cmdline = newSplit(cmdline_str) - - os.system(cmdline_str) - #try: - #check_call(cmdline) - #except CalledProcessError: - # print("Error running the nova-align", file=sys.stderr) + try: + os.system(cmdline_str) + except: + print("Error running the nova-align", file=sys.stderr) def newSplit(value): lex = shlex.shlex(value) @@ -45,7 +36,6 @@ #a dirty way of referencing the file index_file_path = args.index_filename + "/" + args.index_filename.split("/")[-1] - novo_align(args.output_filename, index_file_path, args.forward_filename, args.reverse_filename) if __name__ == "__main__": main()