changeset 1:20c176cd4c3b draft

planemo upload for repository https://github.com/pvanheus/lukasa commit 5f34b6793f0c549e65e25d33b7247c259adedfac
author sanbi-uwc
date Mon, 17 Oct 2022 17:15:15 +0000
parents 7297f56d2d03
children a2eda8fd2737
files lukasa.xml test-data/spaln_out.gff3 test-data/spaln_out_no_st.gff3
diffstat 3 files changed, 68 insertions(+), 69 deletions(-) [+]
line wrap: on
line diff
--- a/lukasa.xml	Tue Oct 13 12:30:39 2020 +0000
+++ b/lukasa.xml	Mon Oct 17 17:15:15 2022 +0000
@@ -1,9 +1,9 @@
 <tool id="lukasa" name="Lukasa" version="@TOOL_VERSION@+galaxy0" python_template_version="3.5">
     <description>Align protein evidence against genomic contigs using metaeuk and spaln</description>
     <macros>
-        <token name="@TOOL_VERSION@">0.0.6</token>
+        <token name="@TOOL_VERSION@">0.10.0</token>
         <!-- note that this DOI is for lukasa 0.0.6 - update as needed -->
-        <token name="@DOI@">10.5281/zenodo.4084863</token> 
+        <token name="@DOI@">10.5281/zenodo.7214490</token>
     </macros>
     <requirements>
         <requirement type="package" version="@TOOL_VERSION@">lukasa</requirement>
@@ -16,24 +16,24 @@
             --output_filename '$output_gff' '$contigs_fasta' '$proteins_fasta'
     ]]></command>
     <inputs>
-        <param name='contigs_fasta' type='data' format='fasta' label='Genomic contigs (FASTA)' />
-        <param name='proteins_fasta' type='data' format='fasta' label='Proteins (FASTA)' />
-        <param name='species_table' type='text' label='Spaln species table to use (optional)' />
+        <param name="contigs_fasta" type="data" format="fasta" label="Genomic contigs (FASTA)"/>
+        <param name="proteins_fasta" type="data" format="fasta" label="Proteins (FASTA)"/>
+        <param name="species_table" type="text" label="Spaln species table to use (optional)"/>
     </inputs>
     <outputs>
-        <data name="output_gff" format='gff3' label='Map proteins to genome ${on_string}' />
+        <data name="output_gff" format="gff3" label="Map proteins to genome ${on_string}"/>
     </outputs>
     <tests>
         <test>
-            <param name="contigs_fasta" ftype="fasta" value="contig.fasta" />
-            <param name="proteins_fasta" ftype="fasta" value="proteins.fasta" />
-            <param name="species_table" value="cynosemi" />
-            <output name="output_gff" value="spaln_out.gff3" />
+            <param name="contigs_fasta" ftype="fasta" value="contig.fasta"/>
+            <param name="proteins_fasta" ftype="fasta" value="proteins.fasta"/>
+            <param name="species_table" value="cynosemi"/>
+            <output name="output_gff" value="spaln_out.gff3"/>
         </test>
         <test>
-            <param name="contigs_fasta" ftype="fasta" value="contig.fasta" />
-            <param name="proteins_fasta" ftype="fasta" value="proteins.fasta" />
-            <output name="output_gff" value="spaln_out_no_st.gff3" />
+            <param name="contigs_fasta" ftype="fasta" value="contig.fasta"/>
+            <param name="proteins_fasta" ftype="fasta" value="proteins.fasta"/>
+            <output name="output_gff" value="spaln_out_no_st.gff3"/>
         </test>
     </tests>
     <help><![CDATA[
@@ -50,4 +50,4 @@
     <citations>
         <citation type="doi">@DOI@</citation>
     </citations>
-</tool>
\ No newline at end of file
+</tool>
--- a/test-data/spaln_out.gff3	Tue Oct 13 12:30:39 2020 +0000
+++ b/test-data/spaln_out.gff3	Mon Oct 17 17:15:15 2022 +0000
@@ -1,56 +1,56 @@
 ##gff-version	3
 ##sequence-region	NW_017366533.1 906 39583
-NW_017366533.1	ALN	gene	1001	3313	1509	+	.	ID=gene00001;Name=NW_017366533.1_1
-NW_017366533.1	ALN	mRNA	1001	3313	1509	+	.	ID=mRNA1_00001;Parent=gene00001;Name=NW_017366533.1_1
-NW_017366533.1	ALN	cds	1001	1293	92	+	0	ID=cds1_00001;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 99 152 +
-NW_017366533.1	ALN	cds	1403	1551	206	+	2	ID=cds1_00002;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 153 202 +
-NW_017366533.1	ALN	cds	1659	1802	189	+	0	ID=cds1_00003;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 203 250 +
+NW_017366533.1	ALN	gene	908	3313	1278	+	.	ID=gene00001;Name=NW_017366533.1_1
+NW_017366533.1	ALN	mRNA	908	3313	1278	+	.	ID=mRNA1_00001;Parent=gene00001;Name=NW_017366533.1_1
+NW_017366533.1	ALN	cds	908	1293	-49	+	0	ID=cds1_00001;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 101 152 +
+NW_017366533.1	ALN	cds	1403	1551	207	+	2	ID=cds1_00002;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 153 202 +
+NW_017366533.1	ALN	cds	1659	1802	188	+	0	ID=cds1_00003;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 203 250 +
 NW_017366533.1	ALN	cds	2061	2214	200	+	0	ID=cds1_00004;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 251 301 +
-NW_017366533.1	ALN	cds	2487	2701	312	+	2	ID=cds1_00005;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 302 374 +
-NW_017366533.1	ALN	cds	2783	3313	610	+	0	ID=cds1_00006;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 375 549 +
-NW_017366533.1	ALN	gene	5314	8701	1447	-	.	ID=gene00002;Name=NW_017366533.1_2
-NW_017366533.1	ALN	mRNA	5314	8701	1447	-	.	ID=mRNA2_00001;Parent=gene00002;Name=NW_017366533.1_2
+NW_017366533.1	ALN	cds	2487	2701	311	+	2	ID=cds1_00005;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 302 374 +
+NW_017366533.1	ALN	cds	2783	3313	608	+	0	ID=cds1_00006;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 375 549 +
+NW_017366533.1	ALN	gene	5314	8701	1365	-	.	ID=gene00002;Name=NW_017366533.1_2
+NW_017366533.1	ALN	mRNA	5314	8701	1365	-	.	ID=mRNA2_00001;Parent=gene00002;Name=NW_017366533.1_2
 NW_017366533.1	ALN	cds	8528	8701	267	-	0	ID=cds2_00001;Parent=mRNA2_00001;Name=NW_017366533.1_2;Target=XP_008322079.1 17 74 +
 NW_017366533.1	ALN	cds	7566	7706	184	-	0	ID=cds2_00002;Parent=mRNA2_00001;Name=NW_017366533.1_2;Target=XP_008322079.1 75 121 +
 NW_017366533.1	ALN	cds	7219	7357	242	-	0	ID=cds2_00003;Parent=mRNA2_00001;Name=NW_017366533.1_2;Target=XP_008322079.1 122 167 +
 NW_017366533.1	ALN	cds	6929	7041	177	-	2	ID=cds2_00004;Parent=mRNA2_00001;Name=NW_017366533.1_2;Target=XP_008322079.1 168 205 +
 NW_017366533.1	ALN	cds	6595	6828	385	-	0	ID=cds2_00005;Parent=mRNA2_00001;Name=NW_017366533.1_2;Target=XP_008322079.1 206 284 +
-NW_017366533.1	ALN	cds	6288	6394	177	-	0	ID=cds2_00006;Parent=mRNA2_00001;Name=NW_017366533.1_2;Target=XP_008322079.1 285 320 +
-NW_017366533.1	ALN	cds	5314	5407	172	-	1	ID=cds2_00007;Parent=mRNA2_00001;Name=NW_017366533.1_2;Target=XP_008322079.1 321 351 +
-NW_017366533.1	ALN	gene	10702	20056	1065	+	.	ID=gene00003;Name=NW_017366533.1_3
-NW_017366533.1	ALN	mRNA	10702	20056	1065	+	.	ID=mRNA3_00001;Parent=gene00003;Name=NW_017366533.1_3
+NW_017366533.1	ALN	cds	6288	6394	178	-	0	ID=cds2_00006;Parent=mRNA2_00001;Name=NW_017366533.1_2;Target=XP_008322079.1 285 320 +
+NW_017366533.1	ALN	cds	5314	5407	173	-	1	ID=cds2_00007;Parent=mRNA2_00001;Name=NW_017366533.1_2;Target=XP_008322079.1 321 351 +
+NW_017366533.1	ALN	gene	10702	20056	995	+	.	ID=gene00003;Name=NW_017366533.1_3
+NW_017366533.1	ALN	mRNA	10702	20056	995	+	.	ID=mRNA3_00001;Parent=gene00003;Name=NW_017366533.1_3
 NW_017366533.1	ALN	cds	10702	10827	208	+	0	ID=cds3_00001;Parent=mRNA3_00001;Name=NW_017366533.1_3;Target=XP_008322769.1 31 72 +
 NW_017366533.1	ALN	cds	15802	15903	235	+	0	ID=cds3_00002;Parent=mRNA3_00001;Name=NW_017366533.1_3;Target=XP_008322769.1 73 106 +
 NW_017366533.1	ALN	cds	17604	17706	205	+	0	ID=cds3_00003;Parent=mRNA3_00001;Name=NW_017366533.1_3;Target=XP_008322769.1 107 140 +
-NW_017366533.1	ALN	cds	17792	17881	192	+	2	ID=cds3_00004;Parent=mRNA3_00001;Name=NW_017366533.1_3;Target=XP_008322769.1 141 170 +
-NW_017366533.1	ALN	cds	18376	18542	310	+	2	ID=cds3_00005;Parent=mRNA3_00001;Name=NW_017366533.1_3;Target=XP_008322769.1 171 226 +
+NW_017366533.1	ALN	cds	17792	17881	191	+	2	ID=cds3_00004;Parent=mRNA3_00001;Name=NW_017366533.1_3;Target=XP_008322769.1 141 170 +
+NW_017366533.1	ALN	cds	18376	18542	309	+	2	ID=cds3_00005;Parent=mRNA3_00001;Name=NW_017366533.1_3;Target=XP_008322769.1 171 226 +
 NW_017366533.1	ALN	cds	20021	20056	71	+	0	ID=cds3_00006;Parent=mRNA3_00001;Name=NW_017366533.1_3;Target=XP_008322769.1 227 238 +
-NW_017366533.1	ALN	gene	22057	32752	5551	+	.	ID=gene00004;Name=NW_017366533.1_4
-NW_017366533.1	ALN	mRNA	22057	32752	5551	+	.	ID=mRNA4_00001;Parent=gene00004;Name=NW_017366533.1_4
-NW_017366533.1	ALN	cds	22057	22827	845	+	0	ID=cds4_00001;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 60 308 +
-NW_017366533.1	ALN	cds	23837	24082	263	+	2	ID=cds4_00002;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 309 387 +
+NW_017366533.1	ALN	gene	22041	32752	5385	+	.	ID=gene00004;Name=NW_017366533.1_4
+NW_017366533.1	ALN	mRNA	22041	32752	5385	+	.	ID=mRNA4_00001;Parent=gene00004;Name=NW_017366533.1_4
+NW_017366533.1	ALN	cds	22041	22827	965	+	0	ID=cds4_00001;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 53 308 +
+NW_017366533.1	ALN	cds	23837	24082	264	+	2	ID=cds4_00002;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 309 387 +
 NW_017366533.1	ALN	cds	24229	24392	270	+	2	ID=cds4_00003;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 388 442 +
-NW_017366533.1	ALN	cds	24476	24637	279	+	0	ID=cds4_00004;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 443 496 +
+NW_017366533.1	ALN	cds	24476	24637	280	+	0	ID=cds4_00004;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 443 496 +
 NW_017366533.1	ALN	cds	24718	24791	127	+	0	ID=cds4_00005;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 497 521 +
 NW_017366533.1	ALN	cds	25910	26093	358	+	1	ID=cds4_00006;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 522 582 +
 NW_017366533.1	ALN	cds	26306	26539	433	+	0	ID=cds4_00007;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 583 660 +
 NW_017366533.1	ALN	cds	27205	27296	152	+	0	ID=cds4_00008;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 661 691 +
 NW_017366533.1	ALN	cds	27383	27510	235	+	1	ID=cds4_00009;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 692 733 +
-NW_017366533.1	ALN	cds	27607	27761	271	+	2	ID=cds4_00010;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 734 785 +
+NW_017366533.1	ALN	cds	27607	27761	272	+	2	ID=cds4_00010;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 734 785 +
 NW_017366533.1	ALN	cds	28374	28508	266	+	0	ID=cds4_00011;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 786 830 +
-NW_017366533.1	ALN	cds	28614	28808	351	+	0	ID=cds4_00012;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 831 895 +
+NW_017366533.1	ALN	cds	28614	28808	350	+	0	ID=cds4_00012;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 831 895 +
 NW_017366533.1	ALN	cds	29603	29785	322	+	0	ID=cds4_00013;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 896 956 +
-NW_017366533.1	ALN	cds	29869	30052	278	+	0	ID=cds4_00014;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 957 1020 +
-NW_017366533.1	ALN	cds	30791	30931	224	+	2	ID=cds4_00015;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1021 1070 +
+NW_017366533.1	ALN	cds	29869	30052	264	+	0	ID=cds4_00014;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 957 1023 +
+NW_017366533.1	ALN	cds	30791	30931	239	+	2	ID=cds4_00015;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1024 1070 +
 NW_017366533.1	ALN	cds	31017	31159	249	+	2	ID=cds4_00016;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1071 1118 +
 NW_017366533.1	ALN	cds	31243	31446	346	+	0	ID=cds4_00017;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1119 1186 +
-NW_017366533.1	ALN	cds	31534	31651	207	+	0	ID=cds4_00018;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1187 1225 +
-NW_017366533.1	ALN	cds	32253	32355	196	+	2	ID=cds4_00019;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1226 1260 +
-NW_017366533.1	ALN	cds	32488	32752	323	+	1	ID=cds4_00020;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1261 1348 +
-NW_017366533.1	ALN	gene	34753	39583	2503	+	.	ID=gene00005;Name=NW_017366533.1_5
-NW_017366533.1	ALN	mRNA	34753	39583	2503	+	.	ID=mRNA5_00001;Parent=gene00005;Name=NW_017366533.1_5
+NW_017366533.1	ALN	cds	31534	31651	205	+	0	ID=cds4_00018;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1187 1225 +
+NW_017366533.1	ALN	cds	32253	32355	197	+	2	ID=cds4_00019;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1226 1260 +
+NW_017366533.1	ALN	cds	32488	32752	322	+	1	ID=cds4_00020;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1261 1348 +
+NW_017366533.1	ALN	gene	34753	39583	2351	+	.	ID=gene00005;Name=NW_017366533.1_5
+NW_017366533.1	ALN	mRNA	34753	39583	2351	+	.	ID=mRNA5_00001;Parent=gene00005;Name=NW_017366533.1_5
 NW_017366533.1	ALN	cds	34753	34872	272	+	0	ID=cds5_00001;Parent=mRNA5_00001;Name=NW_017366533.1_5;Target=XP_008322772.1 1 40 +
-NW_017366533.1	ALN	cds	35722	35874	296	+	0	ID=cds5_00002;Parent=mRNA5_00001;Name=NW_017366533.1_5;Target=XP_008322772.1 41 91 +
+NW_017366533.1	ALN	cds	35722	35874	297	+	0	ID=cds5_00002;Parent=mRNA5_00001;Name=NW_017366533.1_5;Target=XP_008322772.1 41 91 +
 NW_017366533.1	ALN	cds	35986	36078	203	+	0	ID=cds5_00003;Parent=mRNA5_00001;Name=NW_017366533.1_5;Target=XP_008322772.1 92 122 +
 NW_017366533.1	ALN	cds	36220	36285	147	+	0	ID=cds5_00004;Parent=mRNA5_00001;Name=NW_017366533.1_5;Target=XP_008322772.1 123 144 +
 NW_017366533.1	ALN	cds	36706	36831	249	+	0	ID=cds5_00005;Parent=mRNA5_00001;Name=NW_017366533.1_5;Target=XP_008322772.1 145 186 +
--- a/test-data/spaln_out_no_st.gff3	Tue Oct 13 12:30:39 2020 +0000
+++ b/test-data/spaln_out_no_st.gff3	Mon Oct 17 17:15:15 2022 +0000
@@ -1,33 +1,33 @@
 ##gff-version	3
 ##sequence-region	NW_017366533.1 906 39583
-NW_017366533.1	ALN	gene	1001	3313	1569	+	.	ID=gene00001;Name=NW_017366533.1_1
-NW_017366533.1	ALN	mRNA	1001	3313	1569	+	.	ID=mRNA1_00001;Parent=gene00001;Name=NW_017366533.1_1
-NW_017366533.1	ALN	cds	1001	1293	93	+	0	ID=cds1_00001;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 99 152 +
+NW_017366533.1	ALN	gene	908	3313	1336	+	.	ID=gene00001;Name=NW_017366533.1_1
+NW_017366533.1	ALN	mRNA	908	3313	1336	+	.	ID=mRNA1_00001;Parent=gene00001;Name=NW_017366533.1_1
+NW_017366533.1	ALN	cds	908	1293	-48	+	0	ID=cds1_00001;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 101 152 +
 NW_017366533.1	ALN	cds	1403	1551	203	+	2	ID=cds1_00002;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 153 202 +
 NW_017366533.1	ALN	cds	1659	1802	187	+	0	ID=cds1_00003;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 203 250 +
 NW_017366533.1	ALN	cds	2061	2214	199	+	0	ID=cds1_00004;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 251 301 +
 NW_017366533.1	ALN	cds	2487	2701	307	+	2	ID=cds1_00005;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 302 374 +
 NW_017366533.1	ALN	cds	2783	3313	609	+	0	ID=cds1_00006;Parent=mRNA1_00001;Name=NW_017366533.1_1;Target=XP_008319618.1 375 549 +
-NW_017366533.1	ALN	gene	5314	8701	1518	-	.	ID=gene00002;Name=NW_017366533.1_2
-NW_017366533.1	ALN	mRNA	5314	8701	1518	-	.	ID=mRNA2_00001;Parent=gene00002;Name=NW_017366533.1_2
+NW_017366533.1	ALN	gene	5314	8701	1427	-	.	ID=gene00002;Name=NW_017366533.1_2
+NW_017366533.1	ALN	mRNA	5314	8701	1427	-	.	ID=mRNA2_00001;Parent=gene00002;Name=NW_017366533.1_2
 NW_017366533.1	ALN	cds	8528	8701	268	-	0	ID=cds2_00001;Parent=mRNA2_00001;Name=NW_017366533.1_2;Target=XP_008322079.1 17 74 +
 NW_017366533.1	ALN	cds	7566	7706	181	-	0	ID=cds2_00002;Parent=mRNA2_00001;Name=NW_017366533.1_2;Target=XP_008322079.1 75 121 +
 NW_017366533.1	ALN	cds	7219	7357	238	-	0	ID=cds2_00003;Parent=mRNA2_00001;Name=NW_017366533.1_2;Target=XP_008322079.1 122 167 +
 NW_017366533.1	ALN	cds	6929	7041	176	-	2	ID=cds2_00004;Parent=mRNA2_00001;Name=NW_017366533.1_2;Target=XP_008322079.1 168 205 +
-NW_017366533.1	ALN	cds	6595	6828	386	-	0	ID=cds2_00005;Parent=mRNA2_00001;Name=NW_017366533.1_2;Target=XP_008322079.1 206 284 +
+NW_017366533.1	ALN	cds	6595	6828	385	-	0	ID=cds2_00005;Parent=mRNA2_00001;Name=NW_017366533.1_2;Target=XP_008322079.1 206 284 +
 NW_017366533.1	ALN	cds	6288	6394	171	-	0	ID=cds2_00006;Parent=mRNA2_00001;Name=NW_017366533.1_2;Target=XP_008322079.1 285 320 +
 NW_017366533.1	ALN	cds	5314	5407	170	-	1	ID=cds2_00007;Parent=mRNA2_00001;Name=NW_017366533.1_2;Target=XP_008322079.1 321 351 +
-NW_017366533.1	ALN	gene	10702	20056	1109	+	.	ID=gene00003;Name=NW_017366533.1_3
-NW_017366533.1	ALN	mRNA	10702	20056	1109	+	.	ID=mRNA3_00001;Parent=gene00003;Name=NW_017366533.1_3
+NW_017366533.1	ALN	gene	10702	20056	1034	+	.	ID=gene00003;Name=NW_017366533.1_3
+NW_017366533.1	ALN	mRNA	10702	20056	1034	+	.	ID=mRNA3_00001;Parent=gene00003;Name=NW_017366533.1_3
 NW_017366533.1	ALN	cds	10702	10827	207	+	0	ID=cds3_00001;Parent=mRNA3_00001;Name=NW_017366533.1_3;Target=XP_008322769.1 31 72 +
 NW_017366533.1	ALN	cds	15802	15903	228	+	0	ID=cds3_00002;Parent=mRNA3_00001;Name=NW_017366533.1_3;Target=XP_008322769.1 73 106 +
 NW_017366533.1	ALN	cds	17604	17706	204	+	0	ID=cds3_00003;Parent=mRNA3_00001;Name=NW_017366533.1_3;Target=XP_008322769.1 107 140 +
-NW_017366533.1	ALN	cds	17792	17881	187	+	2	ID=cds3_00004;Parent=mRNA3_00001;Name=NW_017366533.1_3;Target=XP_008322769.1 141 170 +
+NW_017366533.1	ALN	cds	17792	17881	186	+	2	ID=cds3_00004;Parent=mRNA3_00001;Name=NW_017366533.1_3;Target=XP_008322769.1 141 170 +
 NW_017366533.1	ALN	cds	18376	18542	305	+	2	ID=cds3_00005;Parent=mRNA3_00001;Name=NW_017366533.1_3;Target=XP_008322769.1 171 226 +
 NW_017366533.1	ALN	cds	20021	20056	70	+	0	ID=cds3_00006;Parent=mRNA3_00001;Name=NW_017366533.1_3;Target=XP_008322769.1 227 238 +
-NW_017366533.1	ALN	gene	22057	32752	5799	+	.	ID=gene00004;Name=NW_017366533.1_4
-NW_017366533.1	ALN	mRNA	22057	32752	5799	+	.	ID=mRNA4_00001;Parent=gene00004;Name=NW_017366533.1_4
-NW_017366533.1	ALN	cds	22057	22827	844	+	0	ID=cds4_00001;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 60 308 +
+NW_017366533.1	ALN	gene	22041	32752	5582	+	.	ID=gene00004;Name=NW_017366533.1_4
+NW_017366533.1	ALN	mRNA	22041	32752	5582	+	.	ID=mRNA4_00001;Parent=gene00004;Name=NW_017366533.1_4
+NW_017366533.1	ALN	cds	22041	22827	964	+	0	ID=cds4_00001;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 53 308 +
 NW_017366533.1	ALN	cds	23837	24082	261	+	2	ID=cds4_00002;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 309 387 +
 NW_017366533.1	ALN	cds	24229	24392	271	+	2	ID=cds4_00003;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 388 442 +
 NW_017366533.1	ALN	cds	24476	24637	281	+	0	ID=cds4_00004;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 443 496 +
@@ -39,18 +39,17 @@
 NW_017366533.1	ALN	cds	27607	27761	266	+	2	ID=cds4_00010;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 734 785 +
 NW_017366533.1	ALN	cds	28374	28508	264	+	0	ID=cds4_00011;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 786 830 +
 NW_017366533.1	ALN	cds	28614	28808	349	+	0	ID=cds4_00012;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 831 895 +
-NW_017366533.1	ALN	cds	29603	29785	319	+	0	ID=cds4_00013;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 896 956 +
-NW_017366533.1	ALN	cds	29869	30052	272	+	0	ID=cds4_00014;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 957 1020 +
-NW_017366533.1	ALN	cds	30433	30452	10	+	2	ID=cds4_00015;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1021 1028 +
-NW_017366533.1	ALN	cds	30555	30561	32	+	0	ID=cds4_00016;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1029 1030 +
-NW_017366533.1	ALN	cds	30812	30931	228	+	2	ID=cds4_00017;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1031 1070 +
-NW_017366533.1	ALN	cds	31017	31159	246	+	2	ID=cds4_00018;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1071 1118 +
-NW_017366533.1	ALN	cds	31243	31446	347	+	0	ID=cds4_00019;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1119 1186 +
-NW_017366533.1	ALN	cds	31534	31651	209	+	0	ID=cds4_00020;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1187 1225 +
-NW_017366533.1	ALN	cds	32253	32355	193	+	2	ID=cds4_00021;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1226 1260 +
-NW_017366533.1	ALN	cds	32488	32752	322	+	1	ID=cds4_00022;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1261 1348 +
-NW_017366533.1	ALN	gene	34753	39583	2632	+	.	ID=gene00005;Name=NW_017366533.1_5
-NW_017366533.1	ALN	mRNA	34753	39583	2632	+	.	ID=mRNA5_00001;Parent=gene00005;Name=NW_017366533.1_5
+NW_017366533.1	ALN	cds	29603	29785	318	+	0	ID=cds4_00013;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 896 956 +
+NW_017366533.1	ALN	cds	29869	30052	261	+	0	ID=cds4_00014;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 957 1025 +
+NW_017366533.1	ALN	cds	30744	30758	27	+	2	ID=cds4_00015;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1026 1030 +
+NW_017366533.1	ALN	cds	30812	30931	223	+	2	ID=cds4_00016;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1031 1070 +
+NW_017366533.1	ALN	cds	31017	31159	246	+	2	ID=cds4_00017;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1071 1118 +
+NW_017366533.1	ALN	cds	31243	31446	347	+	0	ID=cds4_00018;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1119 1186 +
+NW_017366533.1	ALN	cds	31534	31651	209	+	0	ID=cds4_00019;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1187 1225 +
+NW_017366533.1	ALN	cds	32253	32355	193	+	2	ID=cds4_00020;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1226 1260 +
+NW_017366533.1	ALN	cds	32488	32752	322	+	1	ID=cds4_00021;Parent=mRNA4_00001;Name=NW_017366533.1_4;Target=XP_008322771.1 1261 1348 +
+NW_017366533.1	ALN	gene	34753	39583	2466	+	.	ID=gene00005;Name=NW_017366533.1_5
+NW_017366533.1	ALN	mRNA	34753	39583	2466	+	.	ID=mRNA5_00001;Parent=gene00005;Name=NW_017366533.1_5
 NW_017366533.1	ALN	cds	34753	34872	273	+	0	ID=cds5_00001;Parent=mRNA5_00001;Name=NW_017366533.1_5;Target=XP_008322772.1 1 40 +
 NW_017366533.1	ALN	cds	35722	35874	297	+	0	ID=cds5_00002;Parent=mRNA5_00001;Name=NW_017366533.1_5;Target=XP_008322772.1 41 91 +
 NW_017366533.1	ALN	cds	35986	36078	203	+	0	ID=cds5_00003;Parent=mRNA5_00001;Name=NW_017366533.1_5;Target=XP_008322772.1 92 122 +
@@ -58,7 +57,7 @@
 NW_017366533.1	ALN	cds	36706	36831	248	+	0	ID=cds5_00005;Parent=mRNA5_00001;Name=NW_017366533.1_5;Target=XP_008322772.1 145 186 +
 NW_017366533.1	ALN	cds	37166	37247	169	+	0	ID=cds5_00006;Parent=mRNA5_00001;Name=NW_017366533.1_5;Target=XP_008322772.1 187 213 +
 NW_017366533.1	ALN	cds	37677	37768	187	+	2	ID=cds5_00007;Parent=mRNA5_00001;Name=NW_017366533.1_5;Target=XP_008322772.1 214 244 +
-NW_017366533.1	ALN	cds	37884	38017	271	+	0	ID=cds5_00008;Parent=mRNA5_00001;Name=NW_017366533.1_5;Target=XP_008322772.1 245 289 +
+NW_017366533.1	ALN	cds	37884	38017	270	+	0	ID=cds5_00008;Parent=mRNA5_00001;Name=NW_017366533.1_5;Target=XP_008322772.1 245 289 +
 NW_017366533.1	ALN	cds	38109	38214	210	+	1	ID=cds5_00009;Parent=mRNA5_00001;Name=NW_017366533.1_5;Target=XP_008322772.1 290 324 +
 NW_017366533.1	ALN	cds	38546	38704	354	+	0	ID=cds5_00010;Parent=mRNA5_00001;Name=NW_017366533.1_5;Target=XP_008322772.1 325 377 +
 NW_017366533.1	ALN	cds	38803	38876	164	+	0	ID=cds5_00011;Parent=mRNA5_00001;Name=NW_017366533.1_5;Target=XP_008322772.1 378 402 +