view extract_aln_ends.xml @ 58:bbbae1ee87e0 draft default tip

fix for flexbar with small data issue

<tool id="extract_aln_ends.py" name="Extract alignment ends." version="0.2.0">
  <description>from SAM or BAM.</description>
  <requirements>
      <requirement type="package" version="2.24">bedtools</requirement>
      <requirement type="package" version="0.7.4">pybedtools</requirement>
      <requirement type="package" version="0.8.3">pysam</requirement>
  </requirements>
  <macros>
    <import>macros.xml</import>
  </macros>
  <expand macro="stdio" />
  <version_command>python $__tool_directory__/extract_aln_ends.py --version</version_command>
  <command interpreter="python"><![CDATA[
extract_aln_ends.py
#if $positional_1 and $positional_1 is not None:
$positional_1
#end if

> $default]]></command>
  <inputs>
    <param area="false" label="Alignments in SAM or BAM format." name="positional_1" type="data" format="sam,bam"/>
  </inputs>
  <outputs>
    <data format="bed" hidden="false" name="default"/>
  </outputs>
  <tests>
    <test>
      <param name="positional_1" value="twomates.sam"/>
      <output name="default" file="tworeads_aln_ends.bed"/>
    </test>
  </tests>
  <help><![CDATA[
Extract alignment ends from bam file.

The resulting bed file contains the outer coordinates of the alignments. The
bed name field is set to the read id and the score field is set to the edit
distance of the alignment. The crosslinked nucleotide is one nt upstream of the
5'-end of the bed entries.

This tool only reports results for alignments that are properly aligned in FR
("forward-reverse") direction.

Input:

* bam file containing alignments (paired-end sequencing)

Output:

* bed6 file containing outer coordinates (sorted by read id)

Author: Daniel Maticzka
Copyright: 2015
License: Apache
Email: [e-mail]
Status: Development
]]></help>
  <expand macro="citations" />
</tool>