view extract_aln_ends.xml @ 10:17603d9eee69 draft

Uploaded

<tool id="extract_aln_ends.py" name="Extract alignment ends" version="0.1.0">
  <description>from a sam file</description>
  <macros>
    <import>macros.xml</import>
  </macros>
  <expand macro="requirements" />
  <expand macro="stdio" />
  <version_command>python extract_aln_ends.py --version</version_command>
  <command interpreter="python"><![CDATA[
extract_aln_ends.py
#if $positional_1 and $positional_1 is not None:
$positional_1
#end if

> $default]]></command>
  <inputs>
    <param area="false" label="Sam input." name="positional_1" type="data" format="sam"/>
  </inputs>
  <outputs>
    <data format="bed" hidden="false" name="default"/>
  </outputs>
  <tests>
    <test>
      <param name="positional_1" value="twomates.sam"/>
      <output name="default" file="tworeads_aln_ends.bed"/>
    </test>
  </tests>
  <help><![CDATA[
Extract alignment ends from sam file.

The resulting bed file contains the outer coordinates of the alignments. The
bed name field is set to the read id and the score field is set to the edit
distance of the alignment. The crosslinked nucleotide is one nt upstream of the
5'-end of the bed entries.

This tool only reports results for alignments that are properly aligned in FR
("forward-reverse") direction.

Input:

* sam file containing alignments (paired-end sequencing)

Output:

* bed6 file containing outer coordinates (sorted by read id)

Author: Daniel Maticzka
Copyright: 2015
License: Apache
Email: [e-mail]
Status: Development
]]></help>
  <expand macro="citations" />
</tool>