amplicon_analysis_pipeline: amplicon_analysis

annotate amplicon_analysis_pipeline.xml @ 33:c4cde1f435f4 draft

planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 283f59740e90fa714c305007af40041ce39a74aa-dirty

author	pjbriggs
date	Wed, 10 Oct 2018 10:57:42 -0400
parents	d94f2d7e8574
children	3512b135ecb0

rev	line source
27 b5e43dfe07ac planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit e4cd2df591ac5b9da5a1731e81f921313ed9e979 pjbriggs parents: 24 diff changeset	1 <tool id="amplicon_analysis_pipeline" name="Amplicon Analysis Pipeline" version="1.2.3.0">
0 b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	2 <description>analyse 16S rRNA data from Illumina Miseq paired-end reads</description>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	3 <requirements>
23 545f23776953 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 993dae6791b28d776b116153e834192435084ef5 pjbriggs parents: 19 diff changeset	4 <!-- non-bioconda dependencies -->
27 b5e43dfe07ac planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit e4cd2df591ac5b9da5a1731e81f921313ed9e979 pjbriggs parents: 24 diff changeset	5 <requirement type="package" version="1.2.3">amplicon_analysis_pipeline</requirement>
23 545f23776953 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 993dae6791b28d776b116153e834192435084ef5 pjbriggs parents: 19 diff changeset	6 <requirement type="package" version="2010-04-29">microbiomeutil-chimeraslayer</requirement>
545f23776953 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 993dae6791b28d776b116153e834192435084ef5 pjbriggs parents: 19 diff changeset	7 <requirement type="package" version="1.2.22">uclust-qiime</requirement>
545f23776953 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 993dae6791b28d776b116153e834192435084ef5 pjbriggs parents: 19 diff changeset	8 <!-- bioconda dependencies -->
5 bbfc9638ba84 First version with (partial) bioconda deps. pjbriggs parents: 4 diff changeset	9 <requirement type="package" version="2.7">python</requirement>
0 b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	10 <requirement type="package" version="1.11">cutadapt</requirement>
5 bbfc9638ba84 First version with (partial) bioconda deps. pjbriggs parents: 4 diff changeset	11 <requirement type="package" version="1.33">sickle-trim</requirement>
bbfc9638ba84 First version with (partial) bioconda deps. pjbriggs parents: 4 diff changeset	12 <requirement type="package" version="1.0">bioawk</requirement>
0 b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	13 <requirement type="package" version="2.8.1">pandaseq</requirement>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	14 <requirement type="package" version="3.5.0">spades</requirement>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	15 <requirement type="package" version="0.11.3">fastqc</requirement>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	16 <requirement type="package" version="1.8.0">qiime</requirement>
33 c4cde1f435f4 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 283f59740e90fa714c305007af40041ce39a74aa-dirty pjbriggs parents: 32 diff changeset	17 <requirement type="package" version="1.0">libgfortran</requirement>
5 bbfc9638ba84 First version with (partial) bioconda deps. pjbriggs parents: 4 diff changeset	18 <requirement type="package" version="2.2.26">blast-legacy</requirement>
0 b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	19 <requirement type="package" version="0.2.4">fasta-splitter</requirement>
5 bbfc9638ba84 First version with (partial) bioconda deps. pjbriggs parents: 4 diff changeset	20 <requirement type="package" version="2.2">rdp_classifier</requirement>
23 545f23776953 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 993dae6791b28d776b116153e834192435084ef5 pjbriggs parents: 19 diff changeset	21 <requirement type="package" version="1.1.3">vsearch</requirement>
27 b5e43dfe07ac planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit e4cd2df591ac5b9da5a1731e81f921313ed9e979 pjbriggs parents: 24 diff changeset	22 <!-- toolshed dependencies -->
6 45db1f97795c planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit e75f078692e313af3295d2d991fcd8d61270242f pjbriggs parents: 5 diff changeset	23 <requirement type="package" version="3.2.1">R</requirement>
0 b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	24 </requirements>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	25 <stdio>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	26 <exit_code range="1:" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	27 </stdio>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	28 <command><![CDATA[
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	29 ## Set the reference database name
2 a898ee628343 Updated to version 1.2.1.0 pjbriggs parents: 0 diff changeset	30 #if $reference_database == "-S"
a898ee628343 Updated to version 1.2.1.0 pjbriggs parents: 0 diff changeset	31 #set reference_database_name = "silva"
a898ee628343 Updated to version 1.2.1.0 pjbriggs parents: 0 diff changeset	32 #else if $reference_database == "-H"
a898ee628343 Updated to version 1.2.1.0 pjbriggs parents: 0 diff changeset	33 #set reference_database_name = "homd"
a898ee628343 Updated to version 1.2.1.0 pjbriggs parents: 0 diff changeset	34 #else
0 b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	35 #set reference_database_name = "gg"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	36 #end if
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	37
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	38 ## Run the amplicon analysis pipeline wrapper
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	39 python $__tool_directory__/amplicon_analysis_pipeline.py
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	40 ## Set options
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	41 #if str( $forward_pcr_primer ) != ""
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	42 -g "$forward_pcr_primer"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	43 #end if
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	44 #if str( $reverse_pcr_primer ) != ""
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	45 -G "$reverse_pcr_primer"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	46 #end if
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	47 #if str( $trimming_threshold ) != ""
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	48 -q $trimming_threshold
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	49 #end if
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	50 #if str( $sliding_window_length ) != ""
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	51 -l $sliding_window_length
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	52 #end if
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	53 #if str( $minimum_overlap ) != ""
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	54 -O $minimum_overlap
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	55 #end if
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	56 #if str( $minimum_length ) != ""
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	57 -L $minimum_length
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	58 #end if
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	59 -P $pipeline
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	60 -r \$AMPLICON_ANALYSIS_REF_DATA_PATH
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	61 #if str( $reference_database ) != ""
2 a898ee628343 Updated to version 1.2.1.0 pjbriggs parents: 0 diff changeset	62 ${reference_database}
0 b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	63 #end if
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	64 #if str($categories_file_in) != 'None'
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	65 -c "${categories_file_in}"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	66 #end if
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	67 ## Input files
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	68 "${metatable_file_in}"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	69 ## FASTQ pairs
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	70 #if str($input_type.pairs_or_collection) == "collection"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	71 #set fastq_pairs = $input_type.fastq_collection
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	72 #else
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	73 #set fastq_pairs = $input_type.fastq_pairs
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	74 #end if
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	75 #for $fq_pair in $fastq_pairs
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	76 "${fq_pair.name}" "${fq_pair.forward}" "${fq_pair.reverse}"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	77 #end for
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	78 &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	79
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	80 ## Collect outputs
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	81 cp Metatable_log/Metatable_mod.txt "${metatable_mod}" &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	82 cp ${pipeline}_OTU_tables/multiplexed_linearized_dereplicated_mc2_repset_nonchimeras_tax_OTU_table.biom "${tax_otu_table_biom_file}" &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	83 cp ${pipeline}_OTU_tables/otus.tre "${otus_tre_file}" &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	84 cp RESULTS/${pipeline}_${reference_database_name}/OTUs_count.txt "${otus_count_file}" &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	85 cp RESULTS/${pipeline}_${reference_database_name}/table_summary.txt "${table_summary_file}" &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	86 cp Multiplexed_files/${pipeline}_pipeline/multiplexed_linearized_dereplicated_mc2_repset_nonchimeras_OTUs.fasta "${dereplicated_nonchimera_otus_fasta}" &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	87 cp QUALITY_CONTROL/Reads_count.txt "$read_counts_out" &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	88 cp fastqc_quality_boxplots.html "${fastqc_quality_boxplots_html}" &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	89
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	90 ## HTML outputs
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	91
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	92 ## OTU table
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	93 mkdir $heatmap_otu_table_html.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	94 cp -r RESULTS/${pipeline}_${reference_database_name}/Heatmap/js $heatmap_otu_table_html.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	95 cp RESULTS/${pipeline}_${reference_database_name}/Heatmap/otu_table.html "${heatmap_otu_table_html}" &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	96
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	97 ## Phylum genus barcharts
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	98 mkdir $phylum_genus_dist_barcharts_html.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	99 cp -r RESULTS/${pipeline}_${reference_database_name}/phylum_genus_charts/charts $phylum_genus_dist_barcharts_html.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	100 cp -r RESULTS/${pipeline}_${reference_database_name}/phylum_genus_charts/raw_data $phylum_genus_dist_barcharts_html.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	101 cp RESULTS/${pipeline}_${reference_database_name}/phylum_genus_charts/bar_charts.html "${phylum_genus_dist_barcharts_html}" &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	102
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	103 ## Beta diversity weighted 2d plots
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	104 mkdir $beta_div_even_weighted_2d_plots.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	105 cp -r RESULTS/${pipeline}_${reference_database_name}/beta_div_even/weighted_2d_plot/* $beta_div_even_weighted_2d_plots.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	106 cp RESULTS/${pipeline}_${reference_database_name}/beta_div_even/weighted_2d_plot/weighted_unifrac_pc_2D_PCoA_plots.html "${beta_div_even_weighted_2d_plots}" &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	107
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	108 ## Beta diversity unweighted 2d plots
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	109 mkdir $beta_div_even_unweighted_2d_plots.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	110 cp -r RESULTS/${pipeline}_${reference_database_name}/beta_div_even/unweighted_2d_plot/* $beta_div_even_unweighted_2d_plots.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	111 cp RESULTS/${pipeline}_${reference_database_name}/beta_div_even/unweighted_2d_plot/unweighted_unifrac_pc_2D_PCoA_plots.html "${beta_div_even_unweighted_2d_plots}" &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	112
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	113 ## Alpha diversity rarefaction plots
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	114 mkdir $alpha_div_rarefaction_plots.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	115 cp RESULTS/${pipeline}_${reference_database_name}/Alpha_diversity/rarefaction_curves/rarefaction_plots.html $alpha_div_rarefaction_plots &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	116 cp -r RESULTS/${pipeline}_${reference_database_name}/Alpha_diversity/rarefaction_curves/average_plots $alpha_div_rarefaction_plots.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	117
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	118 ## Categories data
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	119 #if str($categories_file_in) != 'None'
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	120 ## Alpha diversity boxplots
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	121 mkdir $alpha_div_boxplots.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	122 cp alpha_diversity_boxplots.html "$alpha_div_boxplots" &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	123 cp RESULTS/${pipeline}_${reference_database_name}/Alpha_diversity/Alpha_diversity_boxplot/Categories_shannon/*.pdf $alpha_div_boxplots.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	124 #end if
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	125
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	126 ## Pipeline outputs (log files etc)
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	127 mkdir $log_files.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	128 cp Amplicon_analysis_pipeline.log $log_files.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	129 cp pipeline.log $log_files.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	130 cp Pipeline_outputs.txt $log_files.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	131 cp Metatable_log/Metatable.html $log_files.files_path &&
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	132 cp pipeline_outputs.html "$log_files"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	133 ]]></command>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	134 <inputs>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	135 <param name="title" type="text" value="test" size="25"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	136 label="Title" help="Optional text that will be added to the output dataset names" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	137 <param type="data" name="metatable_file_in" format="tabular"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	138 label="Input Metatable.txt file" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	139 <param type="data" name="categories_file_in" format="txt"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	140 label="Input Categories.txt file" optional="true"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	141 help="(optional)" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	142 <conditional name="input_type">
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	143 <param name="pairs_or_collection" type="select"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	144 label="Input FASTQ type">
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	145 <option value="pairs_of_files">Pairs of datasets</option>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	146 <option value="collection" selected="true">Dataset pairs in a collection</option>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	147 </param>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	148 <when value="collection">
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	149 <param name="fastq_collection" type="data_collection"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	150 format="fastqsanger,fastq" collection_type="list:paired"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	151 label="Collection of FASTQ forward and reverse (R1/R2) pairs"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	152 help="Each FASTQ pair will be treated as one sample; the name of each sample will be taken from the first column of the Metatable file " />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	153 </when>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	154 <when value="pairs_of_files">
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	155 <repeat name="fastq_pairs" title="Input fastq pairs" min="1">
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	156 <param type="text" name="name" value=""
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	157 label="Final name for FASTQ pair" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	158 <param type="data" name="fastq_r1" format="fastqsanger,fastq"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	159 label="FASTQ with forward reads (R1)" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	160 <param type="data" name="fastq_r2" format="fastqsanger,fastq"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	161 label="FASTQ with reverse reads (R2)" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	162 </repeat>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	163 </when>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	164 </conditional>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	165 <param type="text" name="forward_pcr_primer" value=""
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	166 label="Forward PCR primer sequence"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	167 help="Optional; must not include barcode or adapter sequence (-g)" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	168 <param type="text" name="reverse_pcr_primer" value=""
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	169 label="Reverse PCR primer sequence"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	170 help="Optional; must not include barcode or adapter sequence (-G)" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	171 <param type="integer" name="trimming_threshold" value="20"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	172 label="Threshold quality below which read will be trimmed"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	173 help="Phred score; default is 20 (-q)" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	174 <param type="integer" name="minimum_overlap" value="10"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	175 label="Minimum overlap in bp between forward and reverse reads"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	176 help="Default is 10 (-O)" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	177 <param type="integer" name="minimum_length" value="200"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	178 label="Minimum length in bp to keep sequence after overlapping"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	179 help="Default is 200 (-L)" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	180 <param type="integer" name="sliding_window_length" value="10"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	181 label="Minimum length in bp to retain a read after trimming"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	182 help="Supplied to Sickle; default is 10 (-l)" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	183 <param type="select" name="pipeline"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	184 label="Pipeline to use for analysis">
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	185 <option value="Vsearch" selected="true" >Vsearch</option>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	186 <!--
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	187 Remove the QIIME and Uparse options for now
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	188 <option value="QIIME">QIIME</option>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	189 <option value="Uparse">Uparse</option>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	190 -->
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	191 </param>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	192 <param type="select" name="reference_database"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	193 label="Reference database">
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	194 <option value="" selected="true">GreenGenes</option>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	195 <option value="-S">Silva</option>
2 a898ee628343 Updated to version 1.2.1.0 pjbriggs parents: 0 diff changeset	196 <option value="-H">Human Oral Microbiome Database (HOMD)</option>
0 b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	197 </param>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	198 </inputs>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	199 <outputs>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	200 <data format="tabular" name="metatable_mod"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	201 label="${tool.name}:${title} Metatable_mod.txt" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	202 <data format="tabular" name="read_counts_out"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	203 label="${tool.name} (${pipeline}):${title} read counts" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	204 <data format="biom" name="tax_otu_table_biom_file"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	205 label="${tool.name} (${pipeline}):${title} tax OTU table (biom format)" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	206 <data format="tabular" name="otus_tre_file"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	207 label="${tool.name} (${pipeline}):${title} otus.tre" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	208 <data format="html" name="phylum_genus_dist_barcharts_html"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	209 label="${tool.name} (${pipeline}):${title} phylum genus dist barcharts HTML" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	210 <data format="tabular" name="otus_count_file"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	211 label="${tool.name} (${pipeline}):${title} OTUs count file" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	212 <data format="tabular" name="table_summary_file"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	213 label="${tool.name} (${pipeline}):${title} table summary file" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	214 <data format="fasta" name="dereplicated_nonchimera_otus_fasta"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	215 label="${tool.name} (${pipeline}):${title} multiplexed linearized dereplicated mc2 repset nonchimeras OTUs FASTA" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	216 <data format="html" name="fastqc_quality_boxplots_html"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	217 label="${tool.name} (${pipeline}):${title} FastQC per-base quality boxplots HTML" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	218 <data format="html" name="heatmap_otu_table_html"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	219 label="${tool.name} (${pipeline}):${title} heatmap OTU table HTML" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	220 <data format="html" name="beta_div_even_weighted_2d_plots"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	221 label="${tool.name} (${pipeline}):${title} beta diversity weighted 2D plots HTML" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	222 <data format="html" name="beta_div_even_unweighted_2d_plots"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	223 label="${tool.name} (${pipeline}):${title} beta diversity unweighted 2D plots HTML" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	224 <data format="html" name="alpha_div_rarefaction_plots"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	225 label="${tool.name} (${pipeline}):${title} alpha diversity rarefaction plots HTML" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	226 <data format="html" name="alpha_div_boxplots"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	227 label="${tool.name} (${pipeline}):${title} alpha diversity boxplots">
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	228 <filter>categories_file_in is not None</filter>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	229 </data>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	230 <data format="html" name="log_files"
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	231 label="${tool.name} (${pipeline}):${title} log files" />
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	232 </outputs>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	233 <tests>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	234 </tests>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	235 <help><![CDATA[
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	236
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	237 What it does
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	238 ------------
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	239
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	240 This pipeline has been designed for the analysis of 16S rRNA data from
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	241 Illumina Miseq (Casava >= 1.8) paired-end reads.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	242
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	243 Usage
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	244 -----
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	245
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	246 1. Preparation of the mapping file and format of unique sample id
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	247 *****************************************************************
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	248
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	249 Before using the amplicon analysis pipeline it would be necessary to
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	250 follow the steps as below to avoid analysis failures and ensure samples
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	251 are labelled appropriately. Sample names for the labelling are derived
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	252 from the fastq files names that are generated from the sequencing. The
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	253 labels will include everything between the beginning of the name and
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	254 the sample number (from C11 to S19 in Fig. 1)
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	255
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	256 .. image:: Pipeline_description_Fig1.png
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	257 :height: 46
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	258 :width: 382
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	259
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	260 Figure 1
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	261
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	262 If analysing 16S data from multiple runs:
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	263
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	264 The samples from different runs may have identical IDs. For example,
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	265 when sequencing the same samples twice, by chance, these could be at
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	266 the same position in both the runs. This would cause the fastq files
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	267 to have exactly the same IDs (Fig. 2).
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	268
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	269 .. image:: Pipeline_description_Fig2.png
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	270 :height: 100
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	271 :width: 463
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	272
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	273 Figure 2
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	274
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	275 In case of identical sample IDs the pipeline will fail to run and
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	276 generate an error at the beginning of the analysis.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	277
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	278 To avoid having to change the file names, before uploading the files,
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	279 ensure that the samples IDs are not repeated.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	280
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	281 2. To upload the file
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	282 *********************
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	283
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	284 Click on Get Data/Upload File from the Galaxy tool panel on the
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	285 left hand side.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	286
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	287 From the pop-up window, choose how to upload the file. The
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	288 Choose local file option can be used for files up to 4Gb. Fastq files
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	289 from Illumina MiSeq will rarely be bigger than 4Gb and this option is
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	290 recommended.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	291
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	292 After choosing the files click Start to begin the upload. The window can
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	293 now be closed and the files will be uploaded onto the Galaxy server. You
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	294 will see the progress on the ``HISTORY`` panel on the right
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	295 side of the screen. The colour will change from grey (queuing), to yellow
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	296 (uploading) and finally green (uploaded).
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	297
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	298 Once all the files are uploaded, click on the operations on multiple
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	299 datasets icon and select the fastq files that need to be analysed.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	300 Click on the tab For all selected... and on the option
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	301 Build List of Dataset pairs (Fig. 3).
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	302
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	303 .. image:: Pipeline_description_Fig3.png
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	304 :height: 247
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	305 :width: 586
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	306
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	307 Figure 3
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	308
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	309 Change the filter parameter ``_1`` and ``_2`` to be ``_R1`` and ``_R2``.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	310 The fastq files forward R1 and reverse R2 should now appear in the
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	311 corresponding columns.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	312
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	313 Select Autopair. This creates a collection of paired fastq files for
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	314 the forward and reverse reads for each sample. The name of the pairs will
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	315 be the ones used by the pipeline. You are free to change the names at this
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	316 point as long as they are the same used in the Metatable file
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	317 (see section 3).
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	318
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	319 Name the collection and click on create list. This reduces the time
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	320 required to input the forward and reverse reads for each individual sample.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	321
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	322 3. Create the Metatable files
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	323 *****************************
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	324
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	325 Metatable.txt
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	326 ~~~~~~~~~~~~~
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	327
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	328 Click on the list of pairs you just created to see the name of the single
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	329 pairs. The name of the pairs will be the ones used by the pipeline,
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	330 therefore, these are the names that need to be used in the Metatable file.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	331
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	332 The Metatable file has to be in QIIME format. You can find a description
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	333 of it on QIIME website http://qiime.org/documentation/file_formats.html
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	334
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	335 EXAMPLE::
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	336
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	337 #SampleID BarcodeSequence LinkerPrimerSequence Disease Gender Description
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	338 Mock-RUN1 TAAGGCGAGCGTAAGA PsA Male Control
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	339 Mock-RUN2 CGTACTAGGCGTAAGA PsA Male Control
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	340 Mock-RUN3 AGGCAGAAGCGTAAGA PsC Female Control
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	341
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	342 Briefly: the column ``LinkerPrimerSequence`` is empty but it cannot be
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	343 deleted. The header is very important. ``#SampleID``, ``Barcode``,
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	344 ``LinkerPrimerSequence`` and ``Description`` are mandatory. Between
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	345 ``LinkerPrimerSequence`` and ``Description`` you can add as many columns
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	346 as you want. For every column a PCoA plot will be created (see
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	347 Results section). You can create this file in Excel and it will have
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	348 to be saved as ``Text(Tab delimited)``.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	349
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	350 During the analysis the Metatable.txt will be checked to ensure that the
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	351 file has the correct format. If necessary, this will be modified and will
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	352 be available as Metatable_corrected.txt in the history panel. If you are
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	353 going to use the metatable file for any other statistical analyses,
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	354 remember to use the ``Metatable_mod.txt`` one, otherwise the sample
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	355 names might not match!
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	356
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	357 Categories.txt (optional)
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	358 ~~~~~~~~~~~~~~~~~~~~~~~~~
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	359
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	360 This file is required if you want to get box plots for comparison of
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	361 alpha diversity indices (see Results section). The file is a list
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	362 (without header and IN ONE COLUMN) of categories present in the
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	363 Metatable.txt file. THE NAMES YOU ARE USING HAVE TO BE THE SAME AS THE
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	364 ONES USED IN THE METATABLE.TXT. You can create this file in Excel and
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	365 will have to be saved as ``Text(Tab delimited)``.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	366
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	367 EXAMPLE::
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	368
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	369 Disease
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	370 Gender
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	371
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	372 Metatable and categories files can be uploaded using Get Data as done
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	373 with the fatsq files.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	374
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	375 4. Analysis
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	376 ***********
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	377
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	378 Under Amplicon_Analysis_Pipeline
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	379
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	380 * Title Name to distinguish between the runs. It will be shown at
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	381 the beginning of each output file name.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	382
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	383 * Input Metatable.txt file Select the Metatable.txt file related to
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	384 this analysis
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	385
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	386 * Input Categories.txt file (Optional) Select the Categories.txt file
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	387 related to this analysis
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	388
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	389 * Input FASTQ type select Dataset pairs in a collection and, then,
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	390 the collection of pairs you created earlier.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	391
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	392 * Forward/Reverse PCR primer sequence if the PCR primer sequences
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	393 have not been removed from the MiSeq during the fastq creation, they
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	394 have to be removed before the analysis. Insert the PCR primer sequence
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	395 in the corresponding field. DO NOT include any barcode or adapter
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	396 sequence. If the PCR primers have been already trimmed by the MiSeq,
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	397 and you include the sequence in this field, this would lead to an error.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	398 Only include the sequences if still present in the fastq files.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	399
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	400 * Threshold quality below which reads will be trimmed Choose the
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	401 Phred score used by Sickle to trim the reads at the 3’ end.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	402
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	403 * Minimum length to retain a read after trimming If the read length
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	404 after trimming is shorter than a user defined length, the read, along
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	405 with the corresponding read pair, will be discarded.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	406
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	407 * Minimum overlap in bp between forward and reverse reads Choose the
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	408 minimum basepair overlap used by Pandaseq to assemble the reads.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	409 Default is 10.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	410
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	411 * Minimum length in bp to keep a sequence after overlapping Choose the
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	412 minimum sequence length used by Pandaseq to keep a sequence after the
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	413 overlapping. This depends on the expected amplicon length. Default is
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	414 380 (used for V3-V4 16S sequencing; expected length ~440bp)
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	415
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	416 * Pipeline to use for analysis Choose the pipeline to use for OTU
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	417 clustering and chimera removal. The Galaxy tool currently supports
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	418 ``Vsearch`` only. ``Uparse`` and ``QIIME`` are planned to be added
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	419 shortly (the tools are already available for the stand-alone pipeline).
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	420
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	421 * Reference database Choose between ``GreenGenes`` and ``Silva``
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	422 databases for taxa assignment.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	423
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	424 Click on Execute to start the analysis.
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	425
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	426 5. Results
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	427 **********
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	428
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	429 Results are entirely generated using QIIME scripts. The results will
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	430 appear in the History panel when the analysis is completed
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	431
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	432 * Vsearch_tax_OTU_table (biom format) The OTU table in BIOM format
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	433 (http://biom-format.org/)
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	434
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	435 * Vsearch_OTUs.tree Phylogenetic tree constructed using
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	436 ``make_phylogeny.py`` (fasttree) QIIME script
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	437 (http://qiime.org/scripts/make_phylogeny.html)
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	438
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	439 * Vsearch_phylum_genus_dist_barcharts_HTML HTML file with bar
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	440 charts at Phylum, Genus and Species level
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	441 (http://qiime.org/scripts/summarize_taxa.html and
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	442 http://qiime.org/scripts/plot_taxa_summary.html)
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	443
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	444 * Vsearch_OTUs_count_file Summary of OTU counts per sample
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	445 (http://biom-format.org/documentation/summarizing_biom_tables.html)
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	446
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	447 * Vsearch_table_summary_file Summary of sequences counts per sample
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	448 (http://biom-format.org/documentation/summarizing_biom_tables.html)
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	449
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	450 * Vsearch_multiplexed_linearized_dereplicated_mc2_repset_nonchimeras_OTUs.fasta
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	451 Fasta file with OTU sequences
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	452
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	453 * Vsearch_heatmap_OTU_table_HTML Interactive OTU heatmap
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	454 (http://qiime.org/1.8.0/scripts/make_otu_heatmap_html.html )
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	455
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	456 * Vsearch_beta_diversity_weighted_2D_plots_HTML PCoA plots in HTML
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	457 format using weighted Unifrac distance measure. Samples are grouped
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	458 by the column names present in the Metatable file. The samples are
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	459 firstly rarefied to the minimum sequencing depth
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	460 (http://qiime.org/scripts/beta_diversity_through_plots.html )
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	461
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	462 * Vsearch_beta_diversity_unweighted_2D_plots_HTML PCoA plots in HTML
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	463 format using Unweighted Unifrac distance measure. Samples are grouped
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	464 by the column names present in the Metatable file. The samples are
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	465 firstly rarefied to the minimum sequencing depth
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	466 (http://qiime.org/scripts/beta_diversity_through_plots.html )
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	467
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	468 Code availability
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	469 -----------------
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	470
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	471 Code is available at https://github.com/MTutino/Amplicon_analysis
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	472
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	473 Credits
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	474 -------
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	475
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	476 Pipeline author: Mauro Tutino
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	477
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	478 Galaxy tool: Peter Briggs
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	479
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	480 ]]></help>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	481 <citations>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	482 <citation type="bibtex">
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	483 @misc{githubAmplicon_analysis,
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	484 author = {Tutino, Mauro},
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	485 year = {2017},
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	486 title = {Amplicon Analysis Pipeline},
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	487 publisher = {GitHub},
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	488 journal = {GitHub repository},
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	489 url = {https://github.com/MTutino/Amplicon_analysis},
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	490 }</citation>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	491 </citations>
b433086738d6 planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit ba3e5b591407db52a586361efb21927c8171ec0e pjbriggs parents: diff changeset	492 </tool>

Mercurial > repos > pjbriggs > amplicon_analysis_pipeline

annotate amplicon_analysis_pipeline.xml @ 33:c4cde1f435f4 draft