Mercurial > repos > peterjc > tmhmm_and_signalp
annotate tools/protein_analysis/README @ 13:c6e5a737d3a0 draft
Uploaded v0.2.2, was missing some test files
author | peterjc |
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date | Mon, 22 Apr 2013 11:46:20 -0400 |
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1 This package contains Galaxy wrappers for a selection of standalone command |
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2 line protein analysis tools: |
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3 |
7 | 4 * SignalP 3.0, THMHMM 2.0, Promoter 2.0 from the Center for Biological |
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5 Sequence Analysis at the Technical University of Denmark, |
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6 http://www.cbs.dtu.dk/cbs/ |
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7 |
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8 * WoLF PSORT v0.2 from http://wolfpsort.org/ |
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8 | 10 * PSORTb v3 from http://www.psort.org/downloads/index.html |
11 | |
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12 Also, the RXLR motif tool uses SignalP 3.0 and HMMER 2.3.2 internally. |
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13 |
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14 To use these Galaxy wrappers you must first install the command line tools. |
8 | 15 At the time of writing they are all free for academic use, or open source. |
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16 |
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17 These wrappers are copyright 2010-2013 by Peter Cock, James Hutton Institute |
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18 (formerly SCRI, Scottish Crop Research Institute), UK. All rights reserved. |
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19 Contributions/revisions copyright 2011 Konrad Paszkiewicz. All rights reserved. |
7 | 20 See the included LICENCE file for details (an MIT style open source licence). |
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21 |
13 | 22 |
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23 Requirements |
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24 ============ |
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25 |
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26 First install those command line tools you wish to use the wrappers for: |
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27 |
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28 1. Install the command line version of SignalP 3.0 and ensure "signalp" is |
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29 on the PATH, see: http://www.cbs.dtu.dk/services/SignalP/ |
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30 |
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31 2. Install the command line version of TMHMM 2.0 and ensure "tmhmm" is on |
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32 the PATH, see: http://www.cbs.dtu.dk/services/TMHMM/ |
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33 |
7 | 34 3. Install the command line version of Promoter 2.0 and ensure "promoter" is |
35 on the PATH, see: http://www.cbs.dtu.dk/services/Promoter | |
36 | |
37 4. Install the WoLF PSORT v0.2 package, and ensure "runWolfPsortSummary" | |
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38 is on the PATH (we use an extra wrapper script to change to the WoLF PSORT |
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39 directory, run runWolfPsortSummary, and then change back to the original |
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40 directory), see: http://wolfpsort.org/WoLFPSORT_package/version0.2/ |
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41 |
7 | 42 5. Install hmmsearch from HMMER 2.3.2 (the last stable release of HMMER 2) |
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43 but put it on the path under the name hmmsearch2 (allowing it to co-exist |
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44 with HMMER 3), or edit rlxr_motif.py accordingly. |
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45 |
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46 Verify each of the tools is installed and working from the command line |
7 | 47 (when logged in as the Galaxy user if appropriate). |
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48 |
13 | 49 |
7 | 50 Manual Installation |
51 =================== | |
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52 |
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53 1. Create a folder tools/protein_analysis under your Galaxy installation. |
7 | 54 This folder name is not critical, and can be changed if desired - you |
55 must update the paths used in tool_conf.xml to match. | |
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56 |
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57 2. Copy/move the following files (from this archive) there: |
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58 |
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59 tmhmm2.xml (Galaxy tool definition) |
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60 tmhmm2.py (Python wrapper script) |
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61 |
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62 signalp3.xml (Galaxy tool definition) |
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63 signalp3.py (Python wrapper script) |
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64 |
7 | 65 promoter2.xml (Galaxy tool definition) |
66 promoter2.py (Python wrapper script) | |
67 | |
8 | 68 psortb.xml (Galaxy tool definition) |
69 psortb.py (Python wrapper script) | |
70 | |
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71 wolf_psort.xml (Galaxy tool definition) |
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72 wolf_psort.py (Python wrapper script) |
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73 |
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74 rxlr_motifs.xml (Galaxy tool definition) |
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75 rxlr_motifs.py (Python script) |
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76 |
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77 seq_analysis_utils.py (shared Python code) |
7 | 78 LICENCE |
79 README (this file) | |
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80 |
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81 3. Edit your Galaxy conjuration file tool_conf.xml (to use the tools) AND |
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82 also tool_conf.xml.sample (to run the tests) to include the new tools |
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83 by adding: |
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84 |
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85 <section name="Protein sequence analysis" id="protein_analysis"> |
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86 <tool file="protein_analysis/tmhmm2.xml" /> |
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87 <tool file="protein_analysis/signalp3.xml" /> |
8 | 88 <tool file="protein_analysis/psortb.xml" /> |
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89 <tool file="protein_analysis/wolf_psort.xml" /> |
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90 <tool file="protein_analysis/rxlr_motifs.xml" /> |
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91 </section> |
7 | 92 <section name="Nucleotide sequence analysis" id="nucleotide_analysis"> |
93 <tool file="protein_analysis/promoter2.xml" /> | |
94 </section> | |
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95 |
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96 Leave out the lines for any tools you do not wish to use in Galaxy. |
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97 |
13 | 98 4. Copy/move the test-data files (from this archive) to Galaxy's |
99 subfolder test-data. | |
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100 |
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101 5. Run the Galaxy functional tests for these new wrappers with: |
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102 |
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103 ./run_functional_tests.sh -id tmhmm2 |
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104 ./run_functional_tests.sh -id signalp3 |
8 | 105 ./run_functional_tests.sh -id Psortb |
106 ./run_functional_tests.sh -id rxlr_motifs | |
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107 |
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108 Alternatively, this should work (assuming you left the name and id as shown in |
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109 the XML file tool_conf.xml.sample): |
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110 |
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111 ./run_functional_tests.sh -sid Protein_sequence_analysis-protein_analysis |
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112 |
8 | 113 To check the section ID expected, use ./run_functional_tests.sh -list |
114 | |
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115 6. Restart Galaxy and check the new tools are shown and work. |
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116 |
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117 |
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118 History |
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119 ======= |
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120 |
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121 v0.0.1 - Initial release |
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122 v0.0.2 - Corrected some typos in the help text |
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123 - Renamed test output file to use Galaxy convention of *.tabular |
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124 v0.0.3 - Check for tmhmm2 silent failures (no output) |
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125 - Additional unit tests |
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126 v0.0.4 - Ignore comment lines in tmhmm2 output. |
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127 v0.0.5 - Explicitly request tmhmm short output (may not be the default) |
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128 v0.0.6 - Improvement to how sub-jobs are run (should be faster) |
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129 v0.0.7 - Change SignalP default truncation from 60 to 70 to match the |
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130 SignalP webservice. |
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131 v0.0.8 - Added WoLF PSORT wrapper to the suite. |
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132 v0.0.9 - Added our RXLR motifs tool to the suite. |
7 | 133 v0.1.0 - Added Promoter 2.0 wrapper (similar to SignalP & TMHMM wrappers) |
134 - Support Galaxy's <parallelism> tag for SignalP, TMHMM & Promoter | |
135 v0.1.1 - Fixed an error in the header of the tabular output from Promoter | |
136 v0.1.2 - Use the new <stdio> settings in the XML wrappers to catch errors | |
137 - Use SGE style $NSLOTS for thread count (otherwise default to 4) | |
9 | 138 v0.1.3 - Added missing file whisson_et_al_rxlr_eer_cropped.hmm to Tool Shed |
8 | 139 v0.2.0 - Added PSORTb wrapper to the suite, based on earlier work |
140 contributed by Konrad Paszkiewicz. | |
13 | 141 v0.2.1 - Use a script to create the Tool Shed tar-ball (removed some stray |
142 files accidentally included previously via a wildcard). | |
143 v0.2.2 - Include missing test files. | |
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144 |
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145 |
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146 Developers |
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147 ========== |
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148 |
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149 This script and other tools are being developed on the following hg branch: |
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150 http://bitbucket.org/peterjc/galaxy-central/src/tools |
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151 |
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152 This incorporates the previously used hg branch: |
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153 http://bitbucket.org/peterjc/galaxy-central/src/seq_analysis |
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154 |
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Migrated tool version 0.0.3 from old tool shed archive to new tool shed repository
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155 For making the "Galaxy Tool Shed" http://community.g2.bx.psu.edu/ tarball use |
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Migrated tool version 0.0.3 from old tool shed archive to new tool shed repository
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156 the following command from the Galaxy root folder: |
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157 |
13 | 158 $ ./tools/protein_analysis/make_tmhmm_and_signalp.sh |
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159 |
13 | 160 This simplifies ensuring a consistent set of files is bundled each time, |
161 including all the relevant test files. |