Mercurial > repos > peterjc > blast_rbh
annotate tools/blast_rbh/blast_rbh.xml @ 6:e47960bcdccb draft
Uploaded v0.1.0f, exclude tied best hits
| author | peterjc |
|---|---|
| date | Wed, 21 May 2014 06:33:50 -0400 |
| parents | c84b6c21e3d4 |
| children | 493243f8eb88 |
| rev | line source |
|---|---|
| 0 | 1 <tool id="blast_reciprocal_best_hits" name="BLAST Reciprocal Best Hits (RBH)" version="0.1.0"> |
| 2 <description>from two FASTA files</description> | |
| 3 <requirements> | |
| 4 <requirement type="binary">makeblastdb</requirement> | |
| 5 <requirement type="binary">blastp</requirement> | |
| 6 <requirement type="binary">blastn</requirement> | |
| 7 <requirement type="package" version="2.2.29">blast+</requirement> | |
| 8 </requirements> | |
| 9 <version_command interpreter="python"> | |
| 10 blast_rbh.py --version | |
| 11 </version_command> | |
| 12 <command interpreter="python"> | |
| 13 blast_rbh.py "$fasta_a" "$fasta_b" $seq.dbtype | |
| 14 #if $seq.dbtype=="nucl" | |
| 15 $seq.nucl_type | |
| 16 #else | |
| 17 $seq.prot_type | |
| 18 #end if | |
| 19 $identity $q_cover "$output" | |
| 20 </command> | |
| 21 <inputs> | |
| 22 <!-- Galaxy does not have sub-types for protein vs nucletide FASTA --> | |
| 23 <param name="fasta_a" type="data" format="fasta" | |
| 24 label="Genes/proteins from species A" | |
| 25 description="FASTA file, one sequence per gene/protein." /> | |
| 26 <param name="fasta_b" type="data" format="fasta" | |
| 27 label="Genes/proteins from species B" | |
| 28 description="FASTA file, one sequence per gene/protein." /> | |
| 29 <conditional name="seq"> | |
| 30 <param name="dbtype" type="select" label="Molecule type of FASTA inputs"> | |
| 31 <option value="prot">protein</option> | |
| 32 <option value="nucl">nucleotide</option> | |
| 33 </param> | |
| 34 <when value="prot"> | |
| 35 <param name="prot_type" type="select" display="radio" label="Type of BLAST"> | |
| 36 <option value="blastp">blastp - Traditional BLASTP to compare a protein query to a protein database</option> | |
| 37 <option value="blastp-short">blastp-short - BLASTP optimized for queries shorter than 30 residues</option> | |
| 38 </param> | |
| 39 </when> | |
| 40 <when value="nucl"> | |
| 41 <param name="nucl_type" type="select" display="radio" label="Type of BLAST"> | |
| 42 <option value="megablast">megablast - Traditional megablast used to find very similar (e.g., intraspecies or closely related species) sequences</option> | |
| 43 <option value="blastn">blastn - Traditional BLASTN requiring an exact match of 11, for somewhat similar sequences</option> | |
| 44 <option value="blastn-short">blastn-short - BLASTN program optimized for sequences shorter than 50 bases</option> | |
| 45 <option value="dc-megablast">dc-megablast - Discontiguous megablast used to find more distant (e.g., interspecies) sequences</option> | |
|
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46 <option value="tblastx">tblastx - TBLASTX program using translated query against translated database (protein level matches)</option> |
| 0 | 47 </param> |
| 48 </when> | |
| 49 </conditional> | |
| 50 <param name="identity" type="float" value="70" min="0" max="100" | |
| 51 label="Minimum percentage identity for BLAST matches" | |
| 52 help="Default is 70%, use 0 for no filtering." /> | |
| 53 <param name="q_cover" type="float" value="50" min="0" max="100" | |
| 54 label="Minimum percentage query coverage for BLAST matches" | |
| 55 help="Default is 50%, use 0 for no filtering." /> | |
| 56 </inputs> | |
| 57 <outputs> | |
| 58 <data name="output" format="tabular" label="BLAST RBH: $fasta_a.name vs $fasta_b.name" /> | |
| 59 </outputs> | |
| 60 <requirements> | |
| 61 </requirements> | |
| 62 <tests> | |
| 63 <test> | |
| 1 | 64 <param name="fasta_a" value="four_human_proteins.fasta" ftype="fasta"/> |
| 65 <param name="fasta_b" value="rhodopsin_proteins.fasta" ftype="fasta"/> | |
| 66 <param name="dbtype" value="prot"/> | |
| 67 <param name="nucl_type" value="blastp"/> | |
| 68 <param name="identity" value="0.0"/> | |
| 69 <param name="q_cover" value="0.0"/> | |
| 70 <output name="output" file="rbh_blastp_four_human_vs_rhodopsin_proteins.tabular" ftype="tabular"/> | |
| 71 </test> | |
| 72 <test> | |
| 0 | 73 <param name="fasta_a" value="rhodopsin_nucs.fasta" ftype="fasta"/> |
| 74 <param name="fasta_b" value="three_human_mRNA.fasta" ftype="fasta"/> | |
| 75 <param name="dbtype" value="nucl"/> | |
| 76 <param name="nucl_type" value="megablast"/> | |
| 77 <param name="identity" value="0.0"/> | |
| 78 <param name="q_cover" value="0.0"/> | |
| 79 <output name="output" file="rbh_megablast_rhodopsin_nucs_vs_three_human_mRNA.tabular" ftype="tabular"/> | |
| 80 </test> | |
| 81 <test> | |
| 82 <param name="fasta_a" value="rhodopsin_nucs.fasta" ftype="fasta"/> | |
| 83 <param name="fasta_b" value="three_human_mRNA.fasta" ftype="fasta"/> | |
| 84 <param name="dbtype" value="nucl"/> | |
| 85 <param name="nucl_type" value="megablast"/> | |
| 86 <param name="identity" value="92"/> | |
| 87 <param name="q_cover" value="86"/> | |
| 88 <output name="output" file="rbh_megablast_rhodopsin_nucs_vs_three_human_mRNA.tabular" ftype="tabular"/> | |
| 89 </test> | |
| 90 <!-- push the percentage identity over the 92.07% level --> | |
| 91 <test> | |
| 92 <param name="fasta_a" value="rhodopsin_nucs.fasta" ftype="fasta"/> | |
| 93 <param name="fasta_b" value="three_human_mRNA.fasta" ftype="fasta"/> | |
| 94 <param name="dbtype" value="nucl"/> | |
| 95 <param name="nucl_type" value="megablast"/> | |
| 96 <param name="identity" value="92.5"/> | |
| 97 <param name="q_cover" value="86"/> | |
| 98 <output name="output" file="rbh_none.tabular" ftype="tabular"/> | |
| 99 </test> | |
| 100 <!-- push the coverage over the 86% level --> | |
| 101 <test> | |
| 102 <param name="fasta_a" value="rhodopsin_nucs.fasta" ftype="fasta"/> | |
| 103 <param name="fasta_b" value="three_human_mRNA.fasta" ftype="fasta"/> | |
| 104 <param name="dbtype" value="nucl"/> | |
| 105 <param name="nucl_type" value="megablast"/> | |
| 106 <param name="identity" value="92"/> | |
| 107 <param name="q_cover" value="87"/> | |
| 108 <output name="output" file="rbh_none.tabular" ftype="tabular"/> | |
| 109 </test> | |
| 110 <test> | |
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111 <param name="fasta_a" value="rhodopsin_nucs.fasta" ftype="fasta"/> |
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112 <param name="fasta_b" value="three_human_mRNA.fasta" ftype="fasta"/> |
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113 <param name="dbtype" value="nucl"/> |
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114 <param name="nucl_type" value="tblastx"/> |
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115 <param name="identity" value="0.0"/> |
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116 <param name="q_cover" value="0.0"/> |
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117 <output name="output" file="rbh_tblastx_rhodopsin_nucs_vs_three_human_mRNA.tabular" ftype="tabular"/> |
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118 </test> |
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c84b6c21e3d4
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119 <test> |
| 0 | 120 <param name="fasta_a" value="three_human_mRNA.fasta" ftype="fasta"/> |
| 121 <param name="fasta_b" value="rhodopsin_nucs.fasta" ftype="fasta"/> | |
| 122 <param name="dbtype" value="nucl"/> | |
| 123 <param name="nucl_type" value="blastn"/> | |
| 124 <param name="identity" value="0.0"/> | |
| 125 <param name="q_cover" value="0.0"/> | |
| 126 <output name="output" file="rbh_blastn_three_human_mRNA_vs_rhodopsin_nucs.tabular" ftype="tabular"/> | |
| 127 </test> | |
| 6 | 128 <!-- this pair of examples test tied best hits --> |
| 129 <test> | |
| 130 <param name="fasta_a" value="k12_ten_proteins.fasta" ftype="fasta"/> | |
| 131 <param name="fasta_b" value="k12_edited_proteins.fasta" ftype="fasta"/> | |
| 132 <param name="dbtype" value="nprot"/> | |
| 133 <param name="nucl_type" value="blastp"/> | |
| 134 <param name="identity" value="0.0"/> | |
| 135 <param name="q_cover" value="0.0"/> | |
| 136 <output name="output" file="rbh_blastp_k12.tabular" ftype="tabular"/> | |
| 137 </test> | |
| 138 <test> | |
| 139 <param name="fasta_a" value="k12_edited_proteins.fasta" ftype="fasta"/> | |
| 140 <param name="fasta_b" value="k12_ten_proteins.fasta" ftype="fasta"/> | |
| 141 <param name="dbtype" value="nprot"/> | |
| 142 <param name="nucl_type" value="blastp"/> | |
| 143 <param name="identity" value="0.0"/> | |
| 144 <param name="q_cover" value="0.0"/> | |
| 145 <output name="output" file="rbh_blastp_k12.tabular" ftype="tabular"/> | |
| 146 </test> | |
| 0 | 147 </tests> |
| 148 <help> | |
| 149 **What it does** | |
| 150 | |
| 2 | 151 Takes two FASTA files (*species A* and *species B*), builds a BLAST database |
| 0 | 152 for each, runs reciprocal BLAST searchs (*A vs B*, and *B vs A*), optionally |
| 2 | 153 filters the HSPs, and then compiles a list of the reciprocal best hits (RBH). |
| 0 | 154 |
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155 The output from this tool is a tabular file containing multiple columns, with |
| 2 | 156 information about the BLAST matches used: |
| 0 | 157 |
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158 ====== ================================== |
| 0 | 159 Column Description |
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160 ------ ---------------------------------- |
| 2 | 161 1 ID from *species A* |
| 162 2 ID from *species B* | |
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163 3 Length of sequence *A* |
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164 4 Length of sequence *B* |
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165 5 Percentage of sequence *A* covered |
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166 6 Percentage of sequence *B* covered |
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167 7 HSP alignment length |
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168 8 HSP percentage identity |
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169 9 HSP bitscore |
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170 ====== ================================== |
| 2 | 171 |
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172 These values correspond to the ``qseqid``/``sseqid``, ``qlen``/``slen``, |
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173 ``qcovhsp``, ``length``, ``pident`` and ``bitscore`` values in the BLAST+ |
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174 tabular output. |
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175 |
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176 For the alignment length, bitscore and percentage identity the values for |
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177 *A vs B* and *B vs A* are typically the same, so their minimum is shown. |
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178 The coverage values are given by the HSP alignment length divided by the |
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179 sequence length (adjusted by a factor of three for TBLASTX). |
| 0 | 180 |
| 6 | 181 Note that if a sequence has equally scoring top BLAST matches to multiple |
| 182 sequence in the other file, it will not be considered for an RBH. This | |
| 183 can happen following gene duplication, or for (near) identical gene | |
| 184 duplicates. | |
| 185 | |
| 0 | 186 .. class:: warningmark |
| 187 | |
| 188 **Note** | |
| 189 | |
| 190 If you are trying to use BLAST RBH matches to identify candidate orthologues | |
| 191 or transfer annotation, you *must* use a percentage identity and minimum | |
| 192 coverage threshold or similiar. See: | |
| 193 | |
| 194 Punta and Ofran (2008) The Rough Guide to In Silico Function Prediction, | |
| 195 or How To Use Sequence and Structure Information To Predict Protein | |
| 196 Function. PLoS Comput Biol 4(10): e1000160. | |
| 197 http://dx.doi.org/10.1371/journal.pcbi.1000160 | |
| 198 | |
| 199 The defaults are to require 70% sequence identity over the aligned region | |
| 200 (using ``pident`` in the BLAST+ tabular output), and that the HSP alignment | |
| 201 covers at least 50% of the query sequence (using ``qcovhsp`` in the BLAST+ | |
| 202 tabular output). | |
| 203 | |
| 204 | |
| 205 **References** | |
| 206 | |
| 207 A specific paper covering this tool is planned, but please also cite: | |
| 208 | |
| 209 Christiam Camacho et al. (2009). | |
| 210 BLAST+: architecture and applications. | |
| 211 BMC Bioinformatics. 15;10:421. | |
| 212 http://dx.doi.org/10.1186/1471-2105-10-421 | |
| 213 | |
| 214 This wrapper is available to install into other Galaxy Instances via the Galaxy | |
| 215 Tool Shed at http://toolshed.g2.bx.psu.edu/view/peterjc/blast_rbh | |
| 216 </help> | |
| 217 </tool> |