Mercurial > repos > nikos > rna_probing
changeset 10:33e625bef2b9 draft
Uploaded
| author | nikos |
|---|---|
| date | Wed, 05 Nov 2014 09:09:03 -0500 |
| parents | e1d3be08a852 |
| children | f6265e05c55c |
| files | k2n.R normalize.R normalize.xml plot_rna.R plot_rna.xml preprocessing.sh preprocessing.xml summarize_unique_barcodes.sh summarize_unique_barcodes.xml test-data/RNase_P.fa test-data/aligned.bam test-data/barcodes.txt test-data/k2n.txt test-data/read_counts.txt test-data/reads1.fastq test-data/reads2.fastq test-data/trimming_stats.txt test-data/unique_barcodes.txt tool_dependencies.xml |
| diffstat | 19 files changed, 7572 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/k2n.R Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,51 @@ +options(stringAsfactors = FALSE, useFancyQuotes = FALSE) +args <- commandArgs(trailingOnly = TRUE) + +# Read inputs +merged <- read.table( args[1] ) +read_counts <- read.table( args[2] ) + +barcodes_nt <- merged[ do.call( paste, as.list( merged[ ,1:3 ] ) ) %in% do.call( paste, as.list( read_counts[ ( read_counts[ , 4 ] ) <= summary( read_counts[ , 4 ] )[ 5 ], 1:3 ] ) ) , 4 ] + +##make the matrix with the nucleotide freqs per position: +nt_counts <- matrix( nrow = 4, ncol = as.numeric(args[4]) ) +for( h in 1:ncol( nt_counts ) ){ + j <- 1 + for( nt_local in c( "A","C","G","T" ) ) { + nt_counts[ j, h ] <- sum( substr( as.character( barcodes_nt ), h, h) == nt_local ) + j <- j + 1 + } +} + +# Calculate frequencies +nt_freqs <- nt_counts / colSums( nt_counts ) + +nt_values <- list() +for( i in 1:ncol( nt_freqs ) ) { + nt_values[[ i ]] <- nt_freqs[ , i ] +} + + +all_posible_comb <- expand.grid( nt_values ) + +probs <- apply( all_posible_comb, 1, prod ) + +###Create Mf_to_Sf: +results <- c() + +i <- 1 +results[ i ] <- sum( 1 - ( 1 - probs )**i ) + +while( results[ i ] <= as.numeric(args[3]) ) { + i <- i + 1 + results[ i ] <- sum( 1 - ( 1 - probs )**i ) #Mf to Sf +} + +#assign molecules number to unique barcode number: +Uf_to_Mf <- c() +for( i in 1:floor( max( results ) ) ) { +abs( results - i ) -> difference +Uf_to_Mf[ i ] <- which( difference == min( difference ) ) #if you want to know how many molecules underlie n unique barcodes ask Uf_to_Mf[n] +} + +write( Uf_to_Mf, file = args[5] )
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/normalize.R Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,169 @@ +##!/usr/bin/Rscript + +## Setup R error handling to go to stderr +options( show.error.messages = FALSE, error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } ) + +# we need that to not crash galaxy with an UTF8 error on German LC settings. +Sys.setlocale("LC_MESSAGES", "en_US.UTF-8") + +suppressMessages(library('getopt')) + +#get options, using the spec as defined by the enclosed list. +#we read the options from the default: commandArgs(TRUE). +spec = matrix(c( + 'treated', 't', 1, "character", + 'control', 'c', 2, "character", + 'method', 'm', 1, "character", + 'fuComplexity', 'b', 2, "integer", + 'k2nTreated', 'k', 2, "character", + 'k2nControl', 'a', 2, "character", + 'cutoff', 'g', 1, "integer", + 'reference', 'r', 2, "character", + 'compdata', 'h', 2, "character", + 'dtcr', 'd', 0, "logical", + 'dtcrWindow', 'e', 2, "integer", + 'dtcrToZero', 'f', 2, "character", + 'slograt', 's', 0, "logical", + 'slogratWindow', 'p', 2, "integer", + 'depthCorrection', 'q', 2, "character", + 'pseudocount', 'l', 2, "integer", + 'swinsor', 'w', 0, "logical", + 'swinsorWindow', 'x', 2, "integer", + 'winsorLevel', 'y', 2, "double", + 'fixQuantile', 'z', 2, "character", + 'ntOffset', 'n', 2, "integer", + 'outputDir', 'o', 1, "character", + 'bedgraph', 'v', 2, "character", + 'bed', 'j', 2, "character", + 'genome', 'i', 2, "character", + 'trackName', 'u', 2, "character" +), byrow=TRUE, ncol=4); + +opt = getopt(spec); + +suppressMessages(require(RNAprobR)) + + +#Create output dir +dir.create(opt$outputDir, showWarnings = FALSE, recursive = TRUE) + + +if (opt$method=="counts"){ + treated_euc <- readsamples(opt$treated, euc=opt$method) +} else if (opt$method=="Fu") { + treated_euc <- readsamples(opt$treated, euc=opt$method, m = opt$fuComplexity) +} else { + treated_euc <- readsamples(opt$treated, euc=opt$method, k2n_files=opt$k2nTreated) +} + + +if ( !is.null(opt$reference)){ + comp_treated <- comp(treated_euc, cutoff=opt$cutoff, + fasta_file=opt$reference) +} else { + comp_treated <- comp(treated_euc, cutoff=opt$cutoff) +} + + +#If present, read control file +if ( !is.null(opt$control) && opt$control != 'None'){ + if (opt$method=="counts"){ + control_euc <- readsamples(opt$control, euc=opt$method) + } else if (opt$method=="Fu") { + control_euc <- readsamples(opt$control, euc=opt$method, m = opt$fuComplexity) + } else { + control_euc <- readsamples(opt$control, euc=opt$method, k2n_files=opt$k2nControl) + } + if ( !is.null(opt$reference)){ + comp_control <- comp(control_euc, cutoff=opt$cutoff, + fasta_file=opt$reference) + } else { + comp_control <- comp(control_euc, cutoff=opt$cutoff) + } +} + +#compdata +if ( !is.null(opt$compdata) ) { + + normalized <- compdata(comp_treated, nt_offset=opt$ntOffset) + colnames(mcols(normalized))[1:4] <- paste(colnames(mcols(normalized))[1:4], ".treated", sep="") + + if ( !is.null(opt$control) && opt$control != 'None') { + normalized <- compdata(comp_control, nt_offset=opt$ntOffset, add_to=normalized) + colnames(mcols(normalized))[6:9] <- c("TC.control", "TCR.control", "Cover.control", "PC.control") + } +} + +#dtcr +if ( !is.null(opt$dtcr) ) { + if ( exists("normalized")) { + normalized <- dtcr(comp_control, comp_treated, window_size=opt$dtcrWindow, + nt_offset=opt$ntOffset, bring_to_zero=opt$dtcrToZero, + add_to=normalized) + } + else { + normalized <- dtcr(comp_control, comp_treated, window_size=opt$dtcrWindow, + nt_offset=opt$ntOffset, bring_to_zero=opt$dtcrToZero) + } +} + +#slograt +if ( !is.null(opt$slograt) ) { + if ( exists("normalized")) { + normalized <- slograt(comp_control, comp_treated, window_size=opt$slogratWindow, + nt_offset=opt$ntOffset, depth_correction = opt$depthCorrection, + pseudocount=opt$pseudocount, add_to=normalized) + } + else { + normalized <- slograt(comp_control, comp_treated, window_size=opt$slogratWindow, + nt_offset=opt$ntOffset, depth_correction = opt$depthCorrection, + pseudocount=opt$pseudocount) + } +} + +#swinsor +if ( !is.null(opt$swinsor) ) { + if ( exists("normalized")) { + normalized <- swinsor(comp_treated, winsor_level = opt$winsorLevel, + window_size=opt$swinsorWindow, + only_top=opt$fixQuantile, nt_offset=opt$ntOffset, + add_to=normalized) + } + else { + normalized <- swinsor(comp_treated, winsor_level = opt$winsorLevel, + window_size=opt$swinsorWindow, + only_top=opt$fixQuantile, nt_offset=opt$ntOffset) + } +} + +#bedgraph output +if ( !is.null(opt$bedgraph)) { + if ( !is.null(opt$dtcr)){ + norm2bedgraph(normalized, bed_file = opt$bed, norm_method = "dtcr", + genome_build = opt$genome, + bedgraph_out_file = paste(opt$outputDir, "/dtcr", + sep=""), + track_name = opt$trackName, + track_description = opt$trackDesc) + } + if ( !is.null(opt$slograt)){ + norm2bedgraph(normalized, bed_file = opt$bed, norm_method = "slograt", + genome_build = opt$genome, + bedgraph_out_file = paste(opt$outputDir, "/slograt", + sep=""), + track_name = opt$trackName, + track_description = opt$trackDesc) + } + if ( !is.null(opt$swinsor)){ + norm2bedgraph(normalized, bed_file = opt$bed, norm_method = "swinsor", + genome_build = opt$genome, + bedgraph_out_file = paste(opt$outputDir, "/swinsor", + sep=""), + track_name = opt$trackName, + track_description = opt$trackDesc) + } +} + +output <- GR2norm_df(normalized) +write.table( output, paste(opt$outputDir, "/norm_df.txt", sep = ""), sep = "\t", + quote = F, row.names = F)
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/normalize.xml Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,248 @@ +<tool id="rna_probing_normalize" version="1.0.0" name="Normalize" force_history_refresh="True"> + <description>RNA probing signal</description> + <requirements> + <requirement type="package" version="3.1.1">R_3_1_1</requirement> + <requirement type="R-module">RNAprobR</requirement> + <requirement type="package" version="1.0.0">RNAprobR</requirement> + <requirement type="set_environment">RNA_PROBING_SCRIPT_PATH</requirement> + </requirements> + + <command interpreter="Rscript"> + normalize.R + + -t $treated + -c $control + + --method $euc_method.euc + + #if str($euc_method.euc) == "Fu": + --fuComplexity $euc_method.complexity + #else if str($euc_method.euc) == "HRF-Seq": + --k2nTreated $euc_method.k2n_treated + --k2nControl $euc_method.k2n_control + #end if + + --reference $fasta + + #if str($compdata) == 'yes': + --compdata + #end if + + #if str($dtcr.check) == 'yes': + --dtcr + --dtcrWindow $dtcr.wsize + --dtcrToZero $dtcr.zero + #end if + + #if str($slograt.check) == 'yes': + --slograt + --slogratWindow $slograt.wsize + --depthCorrection $slograt.depth_cor + --pseudocount $slograt.pseudocount + #end if + + #if str($swinsor.check) == 'yes': + --swinsor + --swinsorWindow $swinsor.wsize + --winsorLevel $swinsor.winsor_level + --fixQuantile $swinsor.only_top + #end if + + --ntOffset $nt_offset + --cutoff $cutoff + + #if str($bedgraph.check) == 'yes': + --bedgraph + --bed "$bedgraph.bed_file" + --genome "$bedgraph.genome" + --trackName "$bedgraph.track_name" + #end if + + -o 'output_dir' > /dev/null + + </command> + + <inputs> + <param name="treated" type="data" format="tabular" label="Unique Barcodes/Counts Treated File" help="'Summarize Unique Barcodes' tool output." /> + <param name="control" type="data" format="tabular" optional="True" label="Unique Barcodes/Counts Control File" help="'Summarize Unique Barcodes' tool output." /> + <conditional name="euc_method"> + <param name="euc" type="select" label="EUC* method" help="*Estimated unique counts."> + <option value="counts" selected="True">Counts - Keep unique counts</option> + <option value="Fu">Fu - Fu GK et al. PNAS 2011 (Binomial Distribution) formula</option> + <option value="HRF-Seq">HRF-Seq - as described in Kieplinski and Vinther, NAR 2014</option> + </param> + <when value="counts" /> + <when value="Fu"> + <param name="complexity" type="integer" value="" min="1" label="Random barcode complexity (m)" help="Highest possible number of unique barcodes. e.g For 7 nucleotide, fully degenerate random barcodes (NNNNNNN) then m = 16384 (m=4^7)." /> + </when> + <when value="HRF-Seq"> + <param name="k2n_treated" type="data" format="text" label="k2n Treated File" help="'Summarize Unique Barcodes' output." /> + <param name="k2n_control" type="data" format="text" label="k2n Control File" optional="True" help="'Summarize Unique Barcodes' output." /> + </when> + </conditional> + <param name="fasta" type="data" format="fasta" optional="True" label="Reference Fasta sequence" help="Used to report nucleotide at each position." /> + <param name="compdata" type="boolean" label="Print raw values" checked="True" truevalue="yes" falsevalue="" help="Get Priming Count, Termination Count, Coverage and TCR values" /> + <conditional name="dtcr"> + <param name="check" type="select" label="deltaTCR Normalization" help="Requires Control dataset!"> + <option value="no" selected="True">Do not apply</option> + <option value="yes">Apply</option> + </param> + <when value="no"/> + <when value="yes"> + <param name="wsize" label="Window Size" type="integer" value="3" min="1" size="3" help="Integer. Use only odd numbers to ensure that windows are centred on a nucleotide of interest." /> + <param name="zero" label="Bring to zero" type="boolean" checked="True" truevalue="TRUE" falsevalue="FALSE" help="Replace negative deltaTCR values with 0." /> + </when> + </conditional> + <conditional name="slograt"> + <param name="check" type="select" label="Smooth Log2 Ratio Normalization" help="Requires Control dataset!"> + <option value="no" selected="True">Do not apply</option> + <option value="yes">Apply</option> + </param> + <when value="no"/> + <when value="yes"> + <param name="wsize" label="Window Size" type="integer" value="5" min="1" size="3" help="Integer. Use only odd numbers to ensure that windows are centred on a nucleotide of interest." /> + <param name="depth_cor" type="select" label="Depth Correction"> + <option value="no">No - counts are used as given</option> + <option value="all" selected="True">All - treated counts are multiplied by sum of control counts and divided by sum of treated counts</option> + <option value="RNA">RNA - as in "All" but per RNA basis</option> + </param> + <param name="pseudocount" type="integer" value="5" size="3" label="Pseudocount" help="Pseudocounts to be added to each nucleotide prior to calculating log2 ratio." /> + </when> + </conditional> + <conditional name="swinsor"> + <param name="check" type="select" label="Sliding window Winsorization" help="Doesn't require Control dataset."> + <option value="no" selected="True">Do not apply</option> + <option value="yes">Apply</option> + </param> + <when value="no"/> + <when value="yes"> + <param name="wsize" label="Window Size" type="integer" value="71" min="1" size="3" help="Integer. Use only odd numbers to ensure that windows are centred on a nucleotide of interest." /> + <param name="winsor_level" type="float" value="0.9" min="0.0" max="1.0" size="3" label="Winsor level" help="Indicates where should winsorization boundaries be made. Value 0.9 indicates that top 5% of data will be reduced to 95% quantile and bottom 5% to 5% quantile (default: 0.9)" /> + <param name="only_top" type="boolean" label="Fix quantile" checked="False" truevalue="TRUE" falsevalue="FALSE" help="If checked the the bottom quantile is fixed to 0." /> + </when> + </conditional> + + <param name="cutoff" label="Cutoff Length" type="integer" value="1" min="0" size="3" help="Only inserts of this length or longer will be used for processing." /> + <param name="nt_offset" label="Nucleotide Offset" type="integer" value="1" size="3" help="How many nucleotides before modification does the reverse transcription terminate? e.g. for HRF-Seq offset = 1" /> + + <conditional name="bedgraph"> + <param name="check" type="select" label="Produce BedGraph output" help="Can be displayed directly on UCSC browser. One file per normalisation method." > + <option value="no" selected="True">No</option> + <option value="yes">Yes</option> + </param> + <when value="yes"> + <param name="bed_file" type="data" format="bed" label="Transcripts ins BED format" help="12 column BED file containing trancript definitions." /> + <param name="genome" type="text" label="Genome Build" help="E.g. hg19" /> + <param name="track_name" type="text" label="Track Name" size="20" value="Track Name" /> + </when> + <when value="no" /> + </conditional> +<!-- <param name="plots" type="boolean" checked="False" truevalue="True" falsevalue="False" label="Produce plots" help="pdf format" /> --> + </inputs> + + <outputs> + <data format="tabular" name="normalized" label="${tool.name} on ${on_string} (tabular)" from_work_dir="output_dir/norm_df.txt" /> + <data format="bedgraph" name="bedgraph_dtcr" label="${tool.name} on ${on_string}: dTCR (bedGraph)" from_work_dir="output_dir/dtcr.bedgraph"> + <filter> bedgraph['check'] == 'yes' and dtcr['check'] == 'yes' </filter> + <actions> + <conditional name="bedgraph.check"> + <when value="yes"> + <action type="metadata" name="dbkey"> + <option type="from_param" name="bedgraph.genome" /> + </action> + </when> + </conditional> + </actions> + </data> + <data format="bedgraph" name="bedgraph_slograt" label="${tool.name} on ${on_string}: Smoot Log2ratio (bedGraph)" from_work_dir="output_dir/slograt.bedgraph"> + <filter> bedgraph['check'] == 'yes' and slograt['check'] == 'yes' </filter> + <actions> + <conditional name="bedgraph.check"> + <when value="yes"> + <action type="metadata" name="dbkey"> + <option type="from_param" name="bedgraph.genome" /> + </action> + </when> + </conditional> + </actions> + </data> + <data format="bedgraph" name="bedgraph_swinsor" label="${tool.name} on ${on_string}: Smooth Winsorisation (bedGraph)" from_work_dir="output_dir/swinsor.bedgraph"> + <filter> bedgraph['check'] == 'yes' and swinsor['check'] == 'yes' </filter> + <actions> + <conditional name="bedgraph.check"> + <when value="yes"> + <action type="metadata" name="dbkey"> + <option type="from_param" name="bedgraph.genome" /> + </action> + </when> + </conditional> + </actions> + </data> + </outputs> + + <tests> + </tests> + + <help> +**What it does** + +Normalize tool performs termini based signal detection using summarized counts of sequenced reads (**Read Counts**) or sets of reads carrying the same barcode seqeuence (**Unique Barcodes**). The number of unique barcodes can be converted to Estimated Unique Counts (EUCs) by correcting for the existence of distinct barcodes of the same sequence with (Fu et al., 2011) or without (Kielpinski and Vinther, 2014) the assumption that all barcodes have the same probability of being captured. + +------ + +**Inputs** + +**Unique Barcodes** or **Read Counts** files produced by *Summarize Unique Barcodes* tool for treated and control samples. If deltaTCR of Smooth Log2 Ratio is desired a Control files are required. + +**k2n file(s)** is (are) required if "HRF-Seq" is selected as the "EUC" method. + +**Reference sequences** in FASTA format. Use the file that you produced the read alignment with. + +------ + +**EUC method** + + * **Counts** - Preserves the count of unique barcodes or read counts (depending on the input). It is the only suitable method for reading-in “Read Counts” file and an applicable method when the highest observed unique barcode count is lower than the square root of the count of all possible barcode combinations (Casbon et al., 2011). + + * **Fu** - Allows different barcodes to share the same sequence and assumes equal probability of ligating each barcode sequence (Fu et al., 2011). When selected the value of the random barcode complexity must be specified (e.g. barcode signature NWTRYSNNNN complexity equals 4×2×1×2×2×2×4×4×4×4 = 16384). + + * **HRF-Seq** - Similar to "Fu" but the probability of ligating different barcodes is estimated via observed frequencies of nucleotides at each barcode position (Kielpinski and Vinther, 2014). Requires k2n files for both treated and control samples. + +------ + +**Normalization methods** + +For a detailed description of the methods used read (publication pending) + +------ + +**Outputs** + +A tab seperated file with normalized signal values: + +====== ============================================================================================== +Column Description +------ ---------------------------------------------------------------------------------------------- + 1 Transcript identifier + 2 Position + 3 Nucleotide + 4- Normalized signal value followed by a column with a test significance value (where applicable) +====== ============================================================================================== + +. + +A BedGraph_ file that can be used for direct data display in the `UCSC Browser`_. + +.. _BedGraph: http://genome.ucsc.edu/goldenpath/help/bedgraph.html + +.. _UCSC Browser: http://genome.ucsc.edu/ + + </help> + + <citations> + <citation type="doi">10.1093/nar/gku167</citation> + <citation type="doi">10.1073/pnas.1017621108</citation> + <citation type="doi">10.1093/nar/gkr217</citation> + </citations> + +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/plot_rna.R Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,41 @@ +#Setup R error handling to go to stderr +options( show.error.messages = FALSE, error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } ) + +# we need that to not crash galaxy with an UTF8 error on German LC settings. +Sys.setlocale("LC_MESSAGES", "en_US.UTF-8") + +suppressMessages(library('getopt')) + +#get options, using the spec as defined by the enclosed list. +#we read the options from the default: commandArgs(TRUE). +spec = matrix(c( + 'help' , 'h', 0, "logical", + 'input', 'i', 1, "character", + 'transcript', 't', 1, "character", + 'method', 'm', 2, "character", + 'cutoff', 'c', 2, "double", + 'type', 'p', 2, "character", + 'output', 'o', 1, "character" +), byrow=TRUE, ncol=4); + +opt = getopt(spec); + +suppressMessages(require(RNAprobR)) + +#Read and convert input to GRanges object +data <- read.table(opt$input, header = TRUE) +dataGR <- norm_df2GR(data) + +#Check if given transcript exists in input file +if ( ! opt$transcript %in% data$RNAid ) { stop("Transript not found. Check input file.") } + +pdf(opt$output) +for (method in strsplit(opt$method, ",")[[1]] ) { + + #Check if columns exists in data file + if (! method %in% colnames(data)) { next } + + plotRNA(dataGR, opt$transcript, method, stat_cutoff = opt$cutoff, type = opt$type, + main=paste(opt$transcript,": ", method, sep="")) +} +dev.off()
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/plot_rna.xml Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,72 @@ +<tool id="rna_probing_plot" version="1.0.0" name="RNA Plot" force_history_refresh="True"> + + <description></description> + <requirements> + <requirement type="package" version="3.1.1">R_3_1_1</requirement> + <requirement type="R-module">RNAprobR</requirement> + <requirement type="package" version="1.0.0">RNAprobR</requirement> + <requirement type="set_environment">RNA_PROBING_SCRIPT_PATH</requirement> + </requirements> + + <command interpreter="Rscript"> + plot_rna.R -i $input -t "$transcript" -m $norm_method -c $cutoff -p $plot_type -o $plot > /dev/null + </command> + + <inputs> + <param name="input" type="data" format="tabular" label="Input dataset" help="'Normalize' tool tabular output." /> + + <param name="transcript" type="text" size="20" label="Transcript identifier" help="Select the transcript that the plot should be generated for."> + <validator type="empty_field"/> + </param> + <param name="norm_method" type="select" display="checkboxes" multiple="true" label="Normalization method" help="Column values to be ploted (One plot per selected method)."> + <option value="dtcr">deltaTCR</option> + <option value="slograt">Smooth Log2 Ratio</option> + <option value="swinsor">Sliding window Winsorization</option> + <option value="TC.treated">Termination Count (Treated)</option> + <option value="Cover.treated">Coverage (Treated)</option> + <option value="TCR.treated">TCR (Treated)</option> + <option value="TC.control">Termination Count (Control)</option> + <option value="Cover.control">Coverage (Control)</option> + <option value="TCR.control">TCR (Control)</option> + </param> + <param name="cutoff" type="float" max="1.0" value="0.05" label="Significance level" help="Reports p-values or standard deviation (red asterisks) below the significance level. Set a negative value to print none." /> + <param name="plot_type" type="select" label="Plot type"> + <option value="l">Lines</option> + <option value="h">Histogram</option> + </param> + </inputs> + + <outputs> + <data name="plot" format="pdf" label="${tool.name} on ${on_string}: ${transcript} plot" /> + </outputs> + + <tests> + <test> + </test> + </tests> + + <help> +**What it does** + +Produces plots for a given transcript in the tabular file produced by *Normalize* tool (one plot per selected method). If a selected normalizing method is not included in the input file it simply not be plotted. + +------ + +**Example** + +Input (head):: + + RNAid Pos nt dtcr dtcr.p + 16S_rRNA_E.coli 1 A 0 0.00787957002462928 + 16S_rRNA_E.coli 2 A 0.000606618229204106 2.87738710042044e-07 + 16S_rRNA_E.coli 3 A 0.00170457733442839 0.0242502062681708 + 16S_rRNA_E.coli 4 T 0.00171914631528914 0.504983784068465 + 16S_rRNA_E.coli 5 T 0.00111252808608504 5.26073688354678e-06 + 16S_rRNA_E.coli 6 G 0.00175507669063296 9.92896700324683e-05 + +Output (Significance: 1e-08, type: histogram) + +.. image:: http://people.binf.ku.dk/slm279/plot.png + + </help> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/preprocessing.sh Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,156 @@ +#!/bin/bash + +#################################################################################################### +#Copyright (C) 2014 Lukasz Kielpinski, Nikos Sidiropoulos + +#This program is free software: you can redistribute it and/or modify it under the terms of the +#GNU General Public License as published by the Free Software Foundation, either version 3 of the +#License, or (at your option) any later version. + +#This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without +#even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +#GNU General Public License for more details (http://www.gnu.org/licenses/). +#################################################################################################### + +#Preprocessing workflow - Read debarcoding and trimming. + +function print_help { +cat <<End-of-message +RNA probing data preprocessing. +Read trimming and debarcoding. +------------------------------------- +Input arguments: +-h: Help +-1: Read1 (FASTQ) - Required +-2: Read2 (FASTQ) - Optional +-b: Barcode signature +-t: Trimming length +-o: Output folder (default: "output_dir") +------------------------------------- +Usage : preprocessing.sh -f <READ1> -r <READ2> -b <BARCODE_SEQ> -t <TRIM_LENGTH> -o <output_dir> +End-of-message +exit +} + +#defaults +output_dir="./output_dir" +read2="" +trim_length=0 + +#parse input +while getopts h1:2:b:t:o: myarg +do case "$myarg" in + h) print_help + exit ;; + 1) read1="$OPTARG" ;; #required + 2) read2="$OPTARG" ;; #optional + b) barcode="$OPTARG" ;; #optional + t) trim_length="$OPTARG" ;; #optional + o) output_dir="$OPTARG" ;; #optional + [?]) echo "ERROR: Unknown parameter" + print_help + exit 1 ;; + esac +done + +if [[ -z $read1 ]]; then + echo "ERROR: Read1 file required!" + print_help + exit 1 +fi + +# Create the output folder +mkdir -p $output_dir + +if [ -z "$barcode" ]; then + barcode='' +fi +BAR_LENGTH=`eval echo ${#barcode}` + +################################################################################################### +# Convert the Barcode signature to a regular exression. + +# Example: +# +# Barcode in the oligo used: NWTRYSNNNN +# Which means that each proper read must begin with: NNNN(C|G)(A|G)(C|T)A(A|T)N +# As regex: +# ^[ACGT][ACGT][ACGT][ACGT][CG][AG][CT][A][AT][ACGT] +# Reverse complement the barcode sequence and create a regular expression. + +REV_COMPLEMENT=$(perl -0777ne's/\n //g; tr/ATGCatgcNnYyRrKkMmBbVvDdHh/TACGtacgNnRrYyMmKkVvBbHhDd/; print scalar reverse $_;' <(echo $barcode)) + +REG_EXP=$(sed 's/A/[A]/g;s/C/[C]/g;s/G/[G]/g;s/T/[T]/g;s/R/[AG]/g;s/Y/[CT]/g;s/S/[GC]/g;s/W/[AT]/g;s/K/[GT]/g;s/M/[AC]/g;s/B/[CGT]/g;s/D/[AGT]/g;s/H/[ACT]/g;s/V/[ACG]/g;s/N/[AGCTN]/g;' <<< $REV_COMPLEMENT) + +################################################################################################### +#Remove reads that do not start with the signature (Read1) + +#First awk removes them, second awk removes corresponding quality strings. +#Remove last N nt - may be derived from the random primers. + +awk '{if(NR%4==2){if(/^'"$REG_EXP"'/){print}else{print ""}}else{print}}' $read1 | + +awk 'BEGIN{trim_flag=0; trimming_stats=0; all_processed=0} +{ + if(NR%4==1){print; all_processed++} + if(NR%4==2){if(length($1)==0){trim_flag=1;trimming_stats++}else{trim_flag=0};print} + if(NR%4==3){print} + if(NR%4==0){if(trim_flag==1){print ""}else{print $0}} +}END{print(trimming_stats, all_processed) > "trimming_stats.error"}' | + +awk -v len="${trim_length}" '{if(NR%2==0){print(substr($1,0,length($1)-len))}else{print}}' | + +awk -v len="${BAR_LENGTH}" '{if(NR%2==0 && length($1)<20+len){printf("\n")}else{print}}' | gzip > R1.fastq.gz & + +wait + +mv trimming_stats.error $output_dir/trimming_stats.txt + +################################################################################################### +#Trim primers and possible random barcode + +if [ -z "$read2" ]; then + #single-end + + #Extract the barcode sequence from the first read: + zcat R1.fastq.gz | awk -v len="${BAR_LENGTH}" '{if(NR%2==0 && length($1)<20+len){printf("\n")}else{if(NR%2==0){print(substr($0,len+1,length($0)))}else{print($0)}}}' | awk '{print($1)}' > $output_dir/read1.fastq & + + zcat R1.fastq.gz | awk -v len="${BAR_LENGTH}" '{if(NR%4==1){print($1)}else{if(NR%4==2){print(substr($0,0,len))}}}' | paste - - > $output_dir/barcodes.txt & + + wait + + #Remove temp files + rm R1.fastq.gz + +else + #paired-end + + #Trim primers (Read2) + awk -v len1="${trim_length}" -v len2="${BAR_LENGTH}" '{if(NR%2==0){print(substr($0,len1+1,(length($0)-len1-len2)))}else{print($0)}}' $read2 | + awk '{if(NR%2==0 && length($1)<20){printf("\n")}else{print}}' | gzip > R2.fastq.gz & + + wait + + #Remove empty reads - remove each pair from for which at least one read of the pair got removed (they are problematic when mapping) + + #First define which lines to keep from both fastq files (k for keep, d for discard in the lines_to_keep file) + paste <(zcat R1.fastq.gz) <(zcat R2.fastq.gz) | awk 'BEGIN{OFS="\n"}{if(NR%4==2 && NF==2){print("k","k","k","k")}else{if(NR%4==2 && NF<2){print("d","d","d","d")}}}' > lines_to_keep + + paste lines_to_keep <(zcat R1.fastq.gz) | awk '{if($1=="k")print($2,$3)}' | gzip > R1_readsANDbarcodes.fastq.gz & + + paste lines_to_keep <(zcat R2.fastq.gz) | awk '{if($1=="k")print($2,$3)}' | awk '{print($1)}' > $output_dir/read2.fastq & + + wait + + ######################################################################## + #Extract the barcode sequence from the first read: + zcat R1_readsANDbarcodes.fastq.gz | awk -v len="${BAR_LENGTH}" '{if(NR%2==0 && length($1)<20+len){printf("\n")}else{if(NR%2==0){print(substr($0,len+1,length($0)))}else{print($0)}}}' | awk '{print($1)}' > $output_dir/read1.fastq & + + zcat R1_readsANDbarcodes.fastq.gz | awk -v len="${BAR_LENGTH}" '{if(NR%4==1){print($1)}else{if(NR%4==2){print(substr($0,0,len))}}}' | paste - - > $output_dir/barcodes.txt & + + wait + + #Remove temp files + rm R1_readsANDbarcodes.fastq.gz R1.fastq.gz R2.fastq.gz lines_to_keep + +fi
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/preprocessing.xml Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,136 @@ +<tool id="rna_probing_preprocessing" version="1.0.0" name="Preprocessing" force_history_refresh="True"> + <description>RNA probing data</description> + + <requirements> + <requirement type="package" version="4.1.0">gnu_awk</requirement> + <requirement type="set_environment">RNA_RPOBING_SCRIPT_PATH</requirement> + </requirements> + + <command interpreter="bash"> + preprocessing.sh + + ## check if paired-end + #if str( $library.type ) == "paired" + -2 $library.input2 + #end if + + ## Inputs + -1 $library.input1 + + ## Barcode sequence + -b '$library.barcode_seq' + + ## Trimming length + -t $trim + </command> + + <inputs> + <!-- single/paired --> + <conditional name="library"> + <param name="type" type="select" label="Is this single or paired-end sequencing?"> + <option value="single">Single-end</option> + <option value="paired">Paired-end</option> + </param> + <when value="single"> + <param format="fastqsanger" name="input1" type="data" label="FASTQ file" help="Must have Sanger-scaled quality values (fastqsanger)." /> + <param name="barcode_seq" type="text" size="20" label="Barcode sequence" help="Reads that do not start with the signature will be removed. Use IUPAC alphabet, e.g. NNNNXRTYNN as in the randomized part of the ligation adapter." > + <!-- <validator type="empty_field" message="Specify the Barcode sequence" /> --> + </param> + </when> + <when value="paired"> + <param format="fastqsanger" name="input1" type="data" label="FASTQ file (read 1)" help="Must have Sanger-scaled quality values (fastqsanger)." /> + <param format="fastqsanger" name="input2" type="data" label="FASTQ file (read 2)" help="Must have Sanger-scaled quality values (fastqsanger)." /> + <param name="barcode_seq" type="text" size="20" label="Barcode sequence" help="Reads that do not start with the signature will be removed. Use IUPAC alphabet, e.g. NNNNXRTYNN as in the randomized part of the ligation adapter." > + <!-- <validator type="empty_field" message="Specify the Barcode sequence" /> --> + </param> + </when> + </conditional> + <param name="trim" type="integer" min="0" optional="true" value="15" label="3' trimming length" help="Number of random bases for random priming, will be removed as they are likely to differ from a template." /> + + </inputs> + + <outputs> + <data format="fastqsanger" name="output1" label="${tool.name} on ${on_string}: Read 1" from_work_dir="output_dir/read1.fastq" /> + <data format="fastqsanger" name="output2" label="${tool.name} on ${on_string}: Read 2" from_work_dir="output_dir/read2.fastq" > + <filter> library['type'] == "paired"</filter> + </data> + <data format="tabular" name="barcodes" label="${tool.name} on ${on_string}: Barcodes" from_work_dir="output_dir/barcodes.txt"> + <filter> library['barcode_seq'] != '' </filter> + </data> + </outputs> + + <tests> + <test> + <param name="input1" value="reads1.fastq"/> + <param name="input2" value="reads2.fastq"/> + <param name="barcode_seq" value="NNNNNNN"/> + <param name="trim" value="15"/> + <output name="output1" file="reads1_preprocessed.fastq"/> + <output name="output2" file="reads2_preprocessed.fastq"/> + <output name="barcodes" file="barcodes.txt"/> + </test> + </tests> + + <help> +**What it does** + +*Preprocessing* tool removes and saves the random barcodes sequences, if they were ligated to 3’ ends of cDNA, in a separate dataset to be used in downstream analysis. Additionally to debarcoding, it trims 1) the 5’ end of the second-in-pair reads to remove the reverse transcription primer derived sequence and 2) 3’ end of both reads to remove possible random barcode incorporation in the second-in-pair read and random primer in first-in-pair read. + +------ + +**Examples** + +Sample input files (quality scores omited):: + + * Read1 + @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 1:N:0:ATCACG + TTCGCACAACATNATGGAGGCTTCACGGTACAGAACGAGGCCAGCAAATACCAAGTCTCAGTGAACAAATACAAAGGGACGGCTGGCAACGCCCTCAT + @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 1:N:0:ATCACG + ACCCCGCATCAAATTGGGAACTACTTCCAGCAGTTGTTAGACTTGGGCTCTGGCAGCCCCTTGGAGTGGAGGGACTTGCAGCCCTTCTTATCAGGTCT + + * Read2 + @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 2:N:0:ATCACG + CACAAATCTGCCGTTTGGATTGGCTGCATGGCATCTGTTATACCACCAGCCACCACCATCTTCTTTGGAGCACTGTTTTCTTGGATCCGTAGTTACCC + @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 2:N:0:ATCACG + GTTGGGGGTGTGGGGAAAAAAATAAAAATCGTGAGAAGTTTTAAGACTATGTCACAAAAATGGCTTTAATTATACCATCAAACAGAAACCACCAATTG + +Run 1 - Barcode Sequence = '', Trimming length = 10:: + + * Read1 + @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 + TTCGCACAACATNATGGAGGCTTCACGGTACAGAACGAGGCCAGCAAATACCAAGTCTCAGTGAACAAATACAAAGGGACGGCTGGCA + @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 + ACCCCGCATCAAATTGGGAACTACTTCCAGCAGTTGTTAGACTTGGGCTCTGGCAGCCCCTTGGAGTGGAGGGACTTGCAGCCCTTCT + + * Read2 + @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 + CCGTTTGGATTGGCTGCATGGCATCTGTTATACCACCAGCCACCACCATCTTCTTTGGAGCACTGTTTTCTTGGATCCGTAGTTACCC + @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 + TGGGGAAAAAAATAAAAATCGTGAGAAGTTTTAAGACTATGTCACAAAAATGGCTTTAATTATACCATCAAACAGAAACCACCAATTG + +Run 2 - Barcode Sequence = 'NNNNNNN', Trimming length = 10:: + + * Read1 + @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 + AACATNATGGAGGCTTCACGGTACAGAACGAGGCCAGCAAATACCAAGTCTCAGTGAACAAATACAAAGGGACGGCTGGCA + @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 + ATCAAATTGGGAACTACTTCCAGCAGTTGTTAGACTTGGGCTCTGGCAGCCCCTTGGAGTGGAGGGACTTGCAGCCCTTCT + + * Read2 + @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 + CCGTTTGGATTGGCTGCATGGCATCTGTTATACCACCAGCCACCACCATCTTCTTTGGAGCACTGTTTTCTTGGATCCGTA + @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 + TGGGGAAAAAAATAAAAATCGTGAGAAGTTTTAAGACTATGTCACAAAAATGGCTTTAATTATACCATCAAACAGAAACCA + + * Barcodes + + @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 TTCGCAC + @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 ACCCCGC + + </help> + + <citations> + <citation type="doi">10.1093/nar/gku167</citation> + </citations> + +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/summarize_unique_barcodes.sh Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,226 @@ +#!/bin/bash + +#################################################################################################### +#Copyright (C) 2014 Lukasz Kielpinski, Nikos Sidiropoulos + +#This program is free software: you can redistribute it and/or modify it under the terms of the +#GNU General Public License as published by the Free Software Foundation, either version 3 of the +#License, or (at your option) any later version. + +#This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without +#even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +#GNU General Public License for more details (http://www.gnu.org/licenses/). +#################################################################################################### + +function print_help { +cat <<End-of-message +Summarize Unique Barcodes. +Counts the number of unique random barcodes and reads associated with each sequenced fragment. +------------------------------------- +Input arguments: +-h: Help +-f: Aligned reads in BAM format. +-b: Barcode file (optional). +-p: Set priming position to a fixed value. +-t: Trim untemplated nucleotides. +-k: Produce k2n file. Warning: Can be sloooow! +-r: Rscript path +-o: Output folder (default: "output_dir") +------------------------------------- +Usage : summarize_unique_barcodes.sh -f <BAM_file> -b <BARCODES> -p <PRIMING_POSITION> -t -k -r <R_SCRIPT_PATH> +End-of-message +exit +} + +#defaults +output_dir="output_dir" +trim_flag="False" + +#parse input +while getopts hf:b::p:o:ktr: myarg +do case "$myarg" in + h) print_help + exit ;; + f) bamfile="$OPTARG" ;; #required + b) barcodes="$OPTARG" ;; #optional + t) trim_flag="True" ;; + k) k2n="True" ;; + p) priming_pos="$OPTARG" ;; + o) output_dir="$OPTARG" ;; + r) R_SCRIPT_PATH="$OPTARG" ;; #required + [?]) echo "ERROR: Unknown parameter" + print_help + exit 1 ;; + esac +done + +###### Sanity checks ###### +if [ -z $bamfile ]; then + echo "Error: Aligned reads file is missing!" + print_help + exit 1 +fi + +if [ "$barcodes" == "None" ] && [ "$k2n" == "True" ]; then + echo "Error: k2n file cannot be produced without a barcode file!" + exit 1 +fi + + +mkdir -p $output_dir + +#Check if bamfile contains single or paired-end reads +samtools view -f 0x1 $bamfile | head -n 1 > paired + +if [ -s paired ]; then + #paired-end + samtools view $bamfile | awk 'BEGIN{OFS="\t"}{if(substr($0,1,1)!="@"){print}}' - | awk -v out="${output_dir}/trimming_stats.txt" -v flag="${trim_flag}" 'BEGIN{OFS="\t";counter[0]=0;counter[1]=0;counter[2]=0;counter[3]=0;counter[4]=0} + function abs(value){return(value<0?-value:value)} + function return_offset(local_offset){print($1, $3, $4+local_offset, $4+abs($9)-1);counter[local_offset]++} + ($2 != 99) {next}; + (flag == "False") {return_offset(0);next}; + (/[\s\t]MD:Z:/ && !/MD:Z:([012][ACGT])/) {return_offset(0);next}; + (/[\s\t]MD:Z:0[ACGT]/ && !/MD:Z:0[ACGT][01][ACGT]/ && substr($10,1,1)=="N") {return_offset(0);next}; + (/[\s\t]MD:Z:0[ACGT]/ && !/MD:Z:0[ACGT][01][ACGT]/) {return_offset(1);next}; + + (/[\s\t]MD:Z:1[ACGT]/ && !/MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(0);next}; + (/[\s\t]MD:Z:1[ACGT]/ && !/MD:Z:1[ACGT]0[ACGT]/) {return_offset(2);next}; + + (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,1,2)=="NN") {return_offset(0);next}; + (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,1,1)=="N") {return_offset(2);next}; + (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(1);next}; + (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(2);next}; + + (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,2)=="NN") {return_offset(0);next}; + (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(3);next}; + (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,3,1)=="N") {return_offset(2);next}; + (/[\s\t]MD:Z:1[ACGT]0[ACGT]/) {return_offset(3);next}; + + (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,3,1)=="N" && substr($10,1,1)=="N") {return_offset(0);next}; + (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,3,1)=="N") {return_offset(1);next}; + (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,1,1)=="N") {return_offset(3);next}; + (/MD:Z:0[ACGT]1[ACGT]/) {return_offset(3);next}; + + (/MD:Z:2[ACGT]/ && substr($10,3,1)=="N") {return_offset(0);next}; + (/MD:Z:2[ACGT]/) {return_offset(3);next}; + + (substr($10,3,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(3);next}; + (substr($10,2,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(2);next}; + (substr($10,1,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(1);next}; + + {return_offset(0);counter[4]++} + END{print("No trimming:",counter[0],", out of which not recognized MD field for:",counter[4],"; 1 nt trimmed:", counter[1],"; 2 nt trimmed:", counter[2],"; 3 nt trimmed:",counter[3]) > out}' | sort -S1G -k1,1 | gzip > positions_temp_sorted.gz + +else + #single-end + samtools view $bamfile | awk 'BEGIN{OFS="\t"}{if(substr($0,1,1)!="@"){print}}' - | awk -v out="${output_dir}/trimming_stats.txt" -v flag="${trim_flag}" 'BEGIN{OFS="\t";counter[0]=0;counter[1]=0;counter[2]=0;counter[3]=0;counter[4]=0} + function abs(value){return(value<0?-value:value)} + function return_offset(local_offset){print($1, $3, $4+local_offset, $4+abs($9)-1);counter[local_offset]++} + ($2 != 0) {next}; + (flag == "False") {return_offset(0);next}; + (/[\s\t]MD:Z:/ && !/MD:Z:([012][ACGT])/) {return_offset(0);next}; + (/[\s\t]MD:Z:0[ACGT]/ && !/MD:Z:0[ACGT][01][ACGT]/ && substr($10,1,1)=="N") {return_offset(0);next}; + (/[\s\t]MD:Z:0[ACGT]/ && !/MD:Z:0[ACGT][01][ACGT]/) {return_offset(1);next}; + + (/[\s\t]MD:Z:1[ACGT]/ && !/MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(0);next}; + (/[\s\t]MD:Z:1[ACGT]/ && !/MD:Z:1[ACGT]0[ACGT]/) {return_offset(2);next}; + + (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,1,2)=="NN") {return_offset(0);next}; + (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,1,1)=="N") {return_offset(2);next}; + (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(1);next}; + (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(2);next}; + + (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,2)=="NN") {return_offset(0);next}; + (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(3);next}; + (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,3,1)=="N") {return_offset(2);next}; + (/[\s\t]MD:Z:1[ACGT]0[ACGT]/) {return_offset(3);next}; + + (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,3,1)=="N" && substr($10,1,1)=="N") {return_offset(0);next}; + (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,3,1)=="N") {return_offset(1);next}; + (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,1,1)=="N") {return_offset(3);next}; + (/MD:Z:0[ACGT]1[ACGT]/) {return_offset(3);next}; + + (/MD:Z:2[ACGT]/ && substr($10,3,1)=="N") {return_offset(0);next}; + (/MD:Z:2[ACGT]/) {return_offset(3);next}; + + (substr($10,3,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(3);next}; + (substr($10,2,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(2);next}; + (substr($10,1,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(1);next}; + + {return_offset(0);counter[4]++} + END{print("No trimming:",counter[0],", out of which not recognized MD field for:",counter[4],"; 1 nt trimmed:", counter[1],"; 2 nt trimmed:", counter[2],"; 3 nt trimmed:",counter[3]) > out}' | sort -S1G -k1,1 | gzip > positions_temp_sorted.gz + +fi + +#Computing barcode length (Use the first line and compute the string length of the second column) +if [ "$barcodes" != "None" ]; then + + TMP=`head -1 $barcodes | awk '{print $2}'` + BAR_LEN=`echo ${#TMP}` + + #Remove "@" from barcodes and sort them + sed 's/^.//' $barcodes | sort -k1,1 -S1G | gzip > barcodes_temp_sorted.gz + + #Merge poistions and barcodes + join -1 1 <(zcat positions_temp_sorted.gz) <(zcat barcodes_temp_sorted.gz) | cut -f 2,3,4,5 -d " " | awk '{if($4 !~ /N/){print}}' | awk -v bar_len="${BAR_LEN}" '{if(length($4)==bar_len){print}}' | gzip > merged_temp.gz + + rm barcodes_temp_sorted.gz + +else + zcat positions_temp_sorted.gz | cut -f 2,3,4 | gzip > merged_temp.gz +fi + +#If the experiment is single-end set NA values to the priming column. +if [ ! -s paired ]; then + zcat merged_temp.gz | awk '{print $1, $2, "NA", $4, $5}' - > merged_temp2 + cat merged_temp2 | gzip > merged_temp.gz +fi + +#Fix priming position +if [ ! -z $priming_pos ]; then + zcat merged_temp.gz | awk -v pos="${priming_pos}" '{print $1, $2, pos, $4, $5}' - > merged_temp2 + cat merged_temp2 | gzip > merged_temp.gz +fi + +#File summary.txt columns: RNA_ID, Start, End, barcode sequence, sequenced_count[=number of sequenced fragments fulfilling previous requiremnts] + +zcat merged_temp.gz | awk '{barcode[$1][$2][$3][$4]++}END{ +for(RNA in barcode){ +for(start_position in barcode[RNA]){ +for(end_position in barcode[RNA][start_position]){ +for(barseq in barcode[RNA][start_position][end_position]){print RNA,start_position,end_position,barseq,barcode[RNA][start_position][end_position][barseq]}}}}}' > $output_dir/summary.txt + +#File unique_barcodes.txt columns: RNA_ID, Start, End, number of unique barcodes observed for this fragment. + +awk '{barcode[$1][$2][$3]++}END{ +for(RNA in barcode){ +for(start_position in barcode[RNA]){ +for(end_position in barcode[RNA][start_position]){print RNA "\t" start_position "\t" end_position "\t" barcode[RNA][start_position][end_position]}}}}' $output_dir/summary.txt > $output_dir/unique_barcodes.txt & + +#File read_counts.txt colums: RNA_ID, Start, End, sequenced_count + +zcat merged_temp.gz | awk '{barcode[$1][$2][$3]++}END{ +for(RNA in barcode){ +for(start_position in barcode[RNA]){ +for(end_position in barcode[RNA][start_position]){print RNA "\t" start_position "\t" end_position "\t" barcode[RNA][start_position][end_position]}}}}' > $output_dir/read_counts.txt & + +wait + +#Print the maximum observed barcodes value. Usefull to assess the necessity of producing the k2n file. +if [ "$barcodes" != "None" ]; then + + cut -f 4 $output_dir/unique_barcodes.txt | sort -S1G -rn > sorted_bars + max_observed_barcodes=`head -n 1 sorted_bars` + + echo "Maximum observed Barcodes = ${max_observed_barcodes}" + rm sorted_bars +fi + +#Produce k2n file +if [ "$k2n" == "True" ]; then + bar_length=$(head -n 1 $barcodes | cut -f 2 | xargs expr length) + Rscript $R_SCRIPT_PATH/k2n.R merged_temp.gz $output_dir/read_counts.txt ${max_observed_barcodes} $bar_length $output_dir/k2n.txt +fi + +#Remove temp files +rm paired merged_temp.gz positions_temp_sorted.gz
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/summarize_unique_barcodes.xml Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,137 @@ +<tool id="rna_probing_summarize" version="1.0.0" name="Summarize Unique Barcodes" force_history_refresh="True"> + <description></description> + + <requirements> + <requirement type="package" version="4.1.0">gnu_awk</requirement> + <requirement type="package" version="0.1.19">samtools</requirement> + <requirement type="package" version="3.1.1">R_3_1_1</requirement> + <requirement type="set_environment">RNA_PROBING_SCRIPT_PATH</requirement> + </requirements> + + <command interpreter="bash"> + summarize_unique_barcodes.sh + + ## Inputs + -f $input1 -b $input2 + + ## + + #if str( $k2n ) == 'True': + -k + #end if + + #if str( $priming.flag ) == 'True': + -p $priming.position + #end if + + #if str( $trimming ) == 'True': + -t + #end if + + -r \$RNA_PROBING_SCRIPT_PATH + </command> + + <!-- basic error handling --> + <stdio> + <regex match="Error" level="fatal" description="" /> + </stdio> + + <inputs> + <param format="bam" name="input1" type="data" label="Aligned Reads" help="BAM format." /> + <param format="tabular" name="input2" type="data" optional="True" label="Barcodes" help="Produced by Debarcoding tool." /> + <param name="k2n" type="boolean" checked="False" truevalue="True" falsevalue="False" label="Produce k2n file" help="Check the box if you ran the tool and received a warning message to produce the k2n file. Necessary if you want to use 'HRF-Seq' method in 'Normalize' tool. Warning: Can be very slow!" /> + <param name="trimming" type="boolean" checked="True" truevalue="True" falsevalue="False" label="Trim untemplated nucleotides" help="" /> + <conditional name="priming"> + <param name="flag" type="select" label="Set priming position" help="Set the priming position manually."> + <option value="False">No</option> + <option value="True">Yes</option> + </param> + <when value="True"> + <param name="position" type="integer" value="0" min="0" label="Priming position" /> + </when> + <when value="False" /> + </conditional> + </inputs> + + <outputs> + <data format="tabular" name="trimming_stats" label="${tool.name} on ${on_string}: Trimming stats" from_work_dir="output_dir/trimming_stats.txt"> + <filter>trimming is True</filter> + </data> + <data format="tabular" name="unique_barcodes" label="${tool.name} on ${on_string}: Unique Barcodes" from_work_dir="output_dir/unique_barcodes.txt"> + <filter> input2 != None </filter> + </data> + <data format="tabular" name="read_counts" label="${tool.name} on ${on_string}: Read Counts" from_work_dir="output_dir/read_counts.txt" /> + <data format="txt" name="k2n_file" label="${tool.name} on ${on_string}: k2n file" from_work_dir="output_dir/k2n.txt"> + <filter> k2n is True </filter> + </data> + </outputs> + + <tests> + <test> + <param name="input1" value="aligned.bam" /> + <param name="input2" value="barcodes.txt" /> + <param name="k2n" value="True" /> + <param name="trimming" value="True" /> + <output name="trimming_stats" file="trimming_stats.txt" /> + <output name="unique_barcodes" file="unique_barcodes.txt" /> + <output name="read_counts" file="read_counts.txt" /> + </test> + </tests> + + <help> +**What it does** + +*Summarize Unique Barcodes* counts the number of unique random barcodes and reads associated with each sequenced fragment. A fragment is understood as 1) a pair of Reverse Transcriptase (RT) termination site and RT priming site given paired end sequencing, or 2) an RT termination site in a single end sequencing. For non-barcoded sequencing it only counts the reads matching each fragment. + +------ + +**Inputs** + +*Summarize Unique Barcodes* requires a file containing the Aligned Reads (required) in BAM_ format and tabular file with the Barcodes (optional) produced by the *Preprocessing* tool of the *RNA probing* suite. + +.. _BAM: http://samtools.github.io/hts-specs/SAMv1.pdf + +------- + +**Parameters** + +**Produce k2n file** - A file that contains a sequence of numbers where the n-th element informs how many unique cDNA molecules gives rise to observing n unique barcodes in a given sample. Required for calculating Estimated Unique Counts (EUCs) in tool *Normalize*. + +**Trim untemplated nucleotides** - Untemplated nucleotides can be added to cDNA 3’ ends via terminal transferase activity of reverse transcriptase which offset the location of the read-end mapping and lead to erroneous assignment of reactivity information to nucleotides upstream of those which has reacted (Schmidt and Mueller, 1999, Talkish et al., 2014). Setting this parameter on will remove those nucleotides. + +Recommended for methods based on detecting reverse transcription termination sites (e.g. DMS-Seq, HRF-Seq or SHAPE-Seq), and not for methods based on ligating the linker directly to RNA (e.g. PARS or FragSeq). + +**Set priming position** - Applicable when the priming site is fixed. + +------ + +**Outputs** + +**Unique Barcodes** (if a Barcode file is given) is a tabular file with 4 columns. + +====== ========================================================== +Column Description +------ ---------------------------------------------------------- + 1 Transcript identifier + 2 RT termination site (start) + 3 RT priming site (end) + 4 Count of unique barcodes associated with fragments matching the first three columns +====== ========================================================== + +. + +**Read Counts** is similar to Unique Barcodes but the fourth column is a count of reads matching first three columns. + +**k2n file** as described above. + +**Trimming Stats** reports statistics of trimming untemplated nucleotides from read ends. + + </help> + + <citations> + <citation type="doi">10.1093/nar/gku167</citation> + <citation type="doi">10.1093/nar/27.21.e31-i</citation> + <citation type="doi">10.1261/rna.042218.113</citation> + </citations> + +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/RNase_P.fa Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,4 @@ +>RNase_P +GGGGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATAT +GGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCG +ATCCGCTTCGGCGGATCCAAATCGGGCTTCGGTCCGGTTCAGATCGGAAGAGCACACGTCT
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/barcodes.txt Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,671 @@ +@HWI-ST575:165:C1P5BACXX:1:1101:1666:1132 TCCATCC +@HWI-ST575:165:C1P5BACXX:1:1101:2239:1101 CAACCCC +@HWI-ST575:165:C1P5BACXX:1:1101:2455:1083 CCACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:3207:1238 TGCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:3405:1152 TCAATAC +@HWI-ST575:165:C1P5BACXX:1:1101:3512:1179 TTTCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:4473:1182 GGCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:5162:1092 CTTCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:6810:1177 CGATCAT +@HWI-ST575:165:C1P5BACXX:1:1101:8268:1157 CAAACGG +@HWI-ST575:165:C1P5BACXX:1:1101:8740:1185 GTATGAC +@HWI-ST575:165:C1P5BACXX:1:1101:12414:1099 ATCGGGC +@HWI-ST575:165:C1P5BACXX:1:1101:14053:1112 TATTCTC +@HWI-ST575:165:C1P5BACXX:1:1101:14219:1152 TCGTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:15012:1211 GCCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:15774:1231 ATTACAC +@HWI-ST575:165:C1P5BACXX:1:1101:17047:1127 AATGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:18461:1188 CGATCAC +@HWI-ST575:165:C1P5BACXX:1:1101:19911:1138 TACCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:1311:1500 ACTAGTC +@HWI-ST575:165:C1P5BACXX:1:1101:2110:1396 CCCCCCT +@HWI-ST575:165:C1P5BACXX:1:1101:2970:1430 AGGCGAC +@HWI-ST575:165:C1P5BACXX:1:1101:4079:1444 CTGGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:5495:1254 TCGAACT +@HWI-ST575:165:C1P5BACXX:1:1101:5684:1314 TAATCCT +@HWI-ST575:165:C1P5BACXX:1:1101:6002:1450 GTTGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:6163:1455 TCAATGG +@HWI-ST575:165:C1P5BACXX:1:1101:6370:1342 CAGGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:7500:1303 ACTAAAA +@HWI-ST575:165:C1P5BACXX:1:1101:8186:1308 ATCCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:10049:1341 GATCTGC +@HWI-ST575:165:C1P5BACXX:1:1101:10737:1349 AAGCGAC +@HWI-ST575:165:C1P5BACXX:1:1101:11993:1308 CTGACCC +@HWI-ST575:165:C1P5BACXX:1:1101:12260:1420 TCGGTCG +@HWI-ST575:165:C1P5BACXX:1:1101:13041:1365 CAACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:13915:1290 TCACCCT +@HWI-ST575:165:C1P5BACXX:1:1101:13944:1432 GCAGCTC +@HWI-ST575:165:C1P5BACXX:1:1101:14362:1371 CATCGCA +@HWI-ST575:165:C1P5BACXX:1:1101:14865:1283 CATCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:15341:1459 AACCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:15856:1385 CGATCAC +@HWI-ST575:165:C1P5BACXX:1:1101:16168:1446 CCTATAC +@HWI-ST575:165:C1P5BACXX:1:1101:16274:1448 GACATAC +@HWI-ST575:165:C1P5BACXX:1:1101:17532:1255 GTCAGTC +@HWI-ST575:165:C1P5BACXX:1:1101:19852:1368 ACTCATG +@HWI-ST575:165:C1P5BACXX:1:1101:20338:1414 AACTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:1782:1682 ACTGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:2733:1718 TGCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:2796:1586 TCACACT +@HWI-ST575:165:C1P5BACXX:1:1101:3075:1523 ACTAGTC +@HWI-ST575:165:C1P5BACXX:1:1101:4251:1515 AATACAC +@HWI-ST575:165:C1P5BACXX:1:1101:4481:1718 CATCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:4978:1615 GACGGCC +@HWI-ST575:165:C1P5BACXX:1:1101:6935:1687 AAACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:7393:1637 TGCAGGA +@HWI-ST575:165:C1P5BACXX:1:1101:7744:1585 ACCTTTC +@HWI-ST575:165:C1P5BACXX:1:1101:7646:1624 CTTGTGT +@HWI-ST575:165:C1P5BACXX:1:1101:8080:1642 TTTGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:8452:1710 TCTAGTG +@HWI-ST575:165:C1P5BACXX:1:1101:8575:1513 GAGGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:8610:1649 TCTTACC +@HWI-ST575:165:C1P5BACXX:1:1101:9515:1701 TCCGCCC +@HWI-ST575:165:C1P5BACXX:1:1101:9782:1735 TTACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:10892:1546 CCATTCT +@HWI-ST575:165:C1P5BACXX:1:1101:10875:1631 ATTTGGT +@HWI-ST575:165:C1P5BACXX:1:1101:11536:1555 TTGATGC +@HWI-ST575:165:C1P5BACXX:1:1101:11540:1624 TGGCCGC +@HWI-ST575:165:C1P5BACXX:1:1101:12292:1617 GGAAGTC +@HWI-ST575:165:C1P5BACXX:1:1101:13423:1569 CATCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:13576:1612 CACGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:14491:1526 TGACCAT +@HWI-ST575:165:C1P5BACXX:1:1101:15109:1589 CACTCCT +@HWI-ST575:165:C1P5BACXX:1:1101:16270:1554 TTGATGC +@HWI-ST575:165:C1P5BACXX:1:1101:17059:1656 GGACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:17823:1711 TCCTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:18750:1719 CATATAC +@HWI-ST575:165:C1P5BACXX:1:1101:19397:1535 ATACTAC +@HWI-ST575:165:C1P5BACXX:1:1101:19270:1553 CTGCCAA +@HWI-ST575:165:C1P5BACXX:1:1101:20160:1653 ACACGAC +@HWI-ST575:165:C1P5BACXX:1:1101:20348:1677 GCATCAC +@HWI-ST575:165:C1P5BACXX:1:1101:1152:1999 TCCTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:1575:1782 CCCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:2146:1765 TAATGAC +@HWI-ST575:165:C1P5BACXX:1:1101:2817:1860 CATTGAA +@HWI-ST575:165:C1P5BACXX:1:1101:4121:1770 GCCTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:4015:1808 TTCGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:4671:1789 TCGACAC +@HWI-ST575:165:C1P5BACXX:1:1101:5190:1986 TCGAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:7320:1866 AAACTAC +@HWI-ST575:165:C1P5BACXX:1:1101:7508:1798 GCTAGTT +@HWI-ST575:165:C1P5BACXX:1:1101:9647:1841 TTCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:11078:1760 CGCACTC +@HWI-ST575:165:C1P5BACXX:1:1101:12091:1910 TTACGCA +@HWI-ST575:165:C1P5BACXX:1:1101:13182:1885 ACTGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:13475:1836 AATCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:14275:1813 AGCTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:14914:1754 GCTGTCA +@HWI-ST575:165:C1P5BACXX:1:1101:15826:1814 CAGACCT +@HWI-ST575:165:C1P5BACXX:1:1101:16316:1995 GGGATTC +@HWI-ST575:165:C1P5BACXX:1:1101:17677:1880 CCAAACC +@HWI-ST575:165:C1P5BACXX:1:1101:18640:1881 AACGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:18890:1897 AGGCAGC +@HWI-ST575:165:C1P5BACXX:1:1101:1102:2244 GCCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:2310:2103 GTCTACC +@HWI-ST575:165:C1P5BACXX:1:1101:3289:2146 TACTATT +@HWI-ST575:165:C1P5BACXX:1:1101:4895:2080 TACGAGC +@HWI-ST575:165:C1P5BACXX:1:1101:4932:2108 TATCAAG +@HWI-ST575:165:C1P5BACXX:1:1101:5806:2073 TCCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:6214:2065 GCAAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:6143:2137 TCCACAC +@HWI-ST575:165:C1P5BACXX:1:1101:6241:2199 CGGTCAC +@HWI-ST575:165:C1P5BACXX:1:1101:7894:2030 ATTGTGC +@HWI-ST575:165:C1P5BACXX:1:1101:8072:2245 CCACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:8299:2087 ATACCCC +@HWI-ST575:165:C1P5BACXX:1:1101:8471:2183 ACACATT +@HWI-ST575:165:C1P5BACXX:1:1101:9051:2011 CGGTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:11298:2099 ATTACCC +@HWI-ST575:165:C1P5BACXX:1:1101:11886:2227 AGGTCCC +@HWI-ST575:165:C1P5BACXX:1:1101:12469:2156 CCCGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:13307:2211 GCGGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:13967:2112 CACCATT +@HWI-ST575:165:C1P5BACXX:1:1101:14357:2064 AACCAAA +@HWI-ST575:165:C1P5BACXX:1:1101:14498:2106 TGACTAC +@HWI-ST575:165:C1P5BACXX:1:1101:14632:2166 GGGTCTC +@HWI-ST575:165:C1P5BACXX:1:1101:14873:2011 CCTTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:15054:2152 GCCGGCC +@HWI-ST575:165:C1P5BACXX:1:1101:15855:2031 TCACCAA +@HWI-ST575:165:C1P5BACXX:1:1101:15913:2171 CACCATG +@HWI-ST575:165:C1P5BACXX:1:1101:16436:2126 CACCACC +@HWI-ST575:165:C1P5BACXX:1:1101:16535:2143 CAGGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:16829:2249 CACCATC +@HWI-ST575:165:C1P5BACXX:1:1101:17455:2003 TGGGTCC +@HWI-ST575:165:C1P5BACXX:1:1101:17878:2158 TTCAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:18041:2200 CAGAACT +@HWI-ST575:165:C1P5BACXX:1:1101:2284:2369 CAGACAC +@HWI-ST575:165:C1P5BACXX:1:1101:2836:2439 TCTCTGC +@HWI-ST575:165:C1P5BACXX:1:1101:4292:2260 GACACTT +@HWI-ST575:165:C1P5BACXX:1:1101:4629:2330 TAATCGT +@HWI-ST575:165:C1P5BACXX:1:1101:4537:2454 TGGCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:5316:2437 GTTCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:5705:2286 TAAACAC +@HWI-ST575:165:C1P5BACXX:1:1101:6181:2354 TGCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:6640:2254 GCATCCG +@HWI-ST575:165:C1P5BACXX:1:1101:6520:2397 ACCCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:7019:2438 GATCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:8048:2260 TATTCTC +@HWI-ST575:165:C1P5BACXX:1:1101:8522:2464 GCCCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:8845:2462 GCGGCGG +@HWI-ST575:165:C1P5BACXX:1:1101:9068:2302 ACAACAC +@HWI-ST575:165:C1P5BACXX:1:1101:11110:2418 ATGACTC +@HWI-ST575:165:C1P5BACXX:1:1101:11986:2397 AATCCCT +@HWI-ST575:165:C1P5BACXX:1:1101:12227:2444 TATGACC +@HWI-ST575:165:C1P5BACXX:1:1101:12315:2285 GATGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:12492:2350 ACCAGAT +@HWI-ST575:165:C1P5BACXX:1:1101:13583:2475 CTCAAGC +@HWI-ST575:165:C1P5BACXX:1:1101:14469:2354 ACAAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:14989:2278 GCCATTC +@HWI-ST575:165:C1P5BACXX:1:1101:15188:2338 ACCTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:15331:2257 CACACTT +@HWI-ST575:165:C1P5BACXX:1:1101:15849:2368 ACCGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:16152:2410 TAGCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:16935:2365 GCTCTCC +@HWI-ST575:165:C1P5BACXX:1:1101:17289:2412 CGACCTC +@HWI-ST575:165:C1P5BACXX:1:1101:18384:2478 ACGGACT +@HWI-ST575:165:C1P5BACXX:1:1101:19068:2414 GTGCTAT +@HWI-ST575:165:C1P5BACXX:1:1101:19461:2478 TACTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:19502:2351 GCCAATC +@HWI-ST575:165:C1P5BACXX:1:1101:19741:2372 GCTGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:19565:2447 TAACACC +@HWI-ST575:165:C1P5BACXX:1:1101:20585:2479 CCTGACT +@HWI-ST575:165:C1P5BACXX:1:1101:20888:2259 TGCAAGC +@HWI-ST575:165:C1P5BACXX:1:1101:1310:2696 CAGCCCT +@HWI-ST575:165:C1P5BACXX:1:1101:4969:2645 CCTTAAT +@HWI-ST575:165:C1P5BACXX:1:1101:6485:2694 AAGCCGC +@HWI-ST575:165:C1P5BACXX:1:1101:6896:2699 CCAATAC +@HWI-ST575:165:C1P5BACXX:1:1101:7108:2513 CAACGAC +@HWI-ST575:165:C1P5BACXX:1:1101:7123:2615 TGTTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:7199:2618 TTAATCC +@HWI-ST575:165:C1P5BACXX:1:1101:10015:2714 CGGACTC +@HWI-ST575:165:C1P5BACXX:1:1101:10105:2714 GCTGGTC +@HWI-ST575:165:C1P5BACXX:1:1101:11341:2736 TCTTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:11611:2512 AGACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:11611:2576 GGCTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:11522:2633 CACTATC +@HWI-ST575:165:C1P5BACXX:1:1101:12762:2599 ATCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:13122:2666 AACCGCA +@HWI-ST575:165:C1P5BACXX:1:1101:13331:2504 CTAGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:13328:2614 TAAGCGC +@HWI-ST575:165:C1P5BACXX:1:1101:13944:2746 CCAAACC +@HWI-ST575:165:C1P5BACXX:1:1101:14544:2676 CCTCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:16211:2706 TCATTAC +@HWI-ST575:165:C1P5BACXX:1:1101:17293:2515 CTTACAT +@HWI-ST575:165:C1P5BACXX:1:1101:17341:2572 GAATGGT +@HWI-ST575:165:C1P5BACXX:1:1101:17813:2676 ACACATT +@HWI-ST575:165:C1P5BACXX:1:1101:18322:2589 CCACTCC +@HWI-ST575:165:C1P5BACXX:1:1101:18563:2613 TGCACTC +@HWI-ST575:165:C1P5BACXX:1:1101:19030:2538 ATACCCC +@HWI-ST575:165:C1P5BACXX:1:1101:19375:2502 GTAGTCC +@HWI-ST575:165:C1P5BACXX:1:1101:19630:2588 ACAACCC +@HWI-ST575:165:C1P5BACXX:1:1101:1885:2921 ACGGACC +@HWI-ST575:165:C1P5BACXX:1:1101:3712:2816 GCTTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:3931:2939 TGTTCGG +@HWI-ST575:165:C1P5BACXX:1:1101:4575:2908 AAACTCC +@HWI-ST575:165:C1P5BACXX:1:1101:4670:2972 TTGTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:4779:2823 ACTCGAC +@HWI-ST575:165:C1P5BACXX:1:1101:4804:2998 GTTACTG +@HWI-ST575:165:C1P5BACXX:1:1101:5202:2792 CGCACGC +@HWI-ST575:165:C1P5BACXX:1:1101:5939:2936 CTTGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:6091:2918 CGACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:6392:2828 CGCGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:7180:2991 CATCGGG +@HWI-ST575:165:C1P5BACXX:1:1101:7419:2920 CGTACAC +@HWI-ST575:165:C1P5BACXX:1:1101:7772:2979 GCACCTC +@HWI-ST575:165:C1P5BACXX:1:1101:8507:2811 CGCCGCC +@HWI-ST575:165:C1P5BACXX:1:1101:8832:2869 GCCTCAC +@HWI-ST575:165:C1P5BACXX:1:1101:9485:2898 GCTTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:9990:2821 CATGCTC +@HWI-ST575:165:C1P5BACXX:1:1101:10328:2966 ATCGGTC +@HWI-ST575:165:C1P5BACXX:1:1101:11508:2796 AACGGTC +@HWI-ST575:165:C1P5BACXX:1:1101:11726:2972 GGAGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:12044:2834 CCATGAA +@HWI-ST575:165:C1P5BACXX:1:1101:12169:2963 ACTCCGC +@HWI-ST575:165:C1P5BACXX:1:1101:13168:2760 CGACCTC +@HWI-ST575:165:C1P5BACXX:1:1101:13484:2768 TTTTCTC +@HWI-ST575:165:C1P5BACXX:1:1101:13399:2774 CTAGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:13467:2781 GCATCTC +@HWI-ST575:165:C1P5BACXX:1:1101:13716:2837 CGCTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:14400:2843 CAATTTT +@HWI-ST575:165:C1P5BACXX:1:1101:14816:2802 CACTGTT +@HWI-ST575:165:C1P5BACXX:1:1101:15055:2926 CTAGACG +@HWI-ST575:165:C1P5BACXX:1:1101:17639:2908 TACAGAT +@HWI-ST575:165:C1P5BACXX:1:1101:20319:2983 GGTATTT +@HWI-ST575:165:C1P5BACXX:1:1101:20783:2769 AAAACGC +@HWI-ST575:165:C1P5BACXX:1:1101:1673:3017 AATATCC +@HWI-ST575:165:C1P5BACXX:1:1101:2153:3048 ATTCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:4715:3184 TCCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:4796:3234 CAACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:5104:3008 ACGGCTC +@HWI-ST575:165:C1P5BACXX:1:1101:5272:3008 GAAGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:5594:3119 ACCCCCC +@HWI-ST575:165:C1P5BACXX:1:1101:6927:3052 CACAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:7098:3130 AGCCTCC +@HWI-ST575:165:C1P5BACXX:1:1101:7842:3018 AACCACT +@HWI-ST575:165:C1P5BACXX:1:1101:8316:3056 CTCTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:8582:3236 CCAAAGC +@HWI-ST575:165:C1P5BACXX:1:1101:10343:3076 TTCATAC +@HWI-ST575:165:C1P5BACXX:1:1101:10433:3184 CCCTTTT +@HWI-ST575:165:C1P5BACXX:1:1101:10787:3078 AGCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:10951:3116 GGTCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:11196:3016 TGCTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:11257:3175 TTTTGGT +@HWI-ST575:165:C1P5BACXX:1:1101:12061:3148 CCAAGTC +@HWI-ST575:165:C1P5BACXX:1:1101:12768:3208 ATTCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:14168:3032 TCCCCTC +@HWI-ST575:165:C1P5BACXX:1:1101:14033:3072 GCAACGG +@HWI-ST575:165:C1P5BACXX:1:1101:14598:3012 TGTTTTC +@HWI-ST575:165:C1P5BACXX:1:1101:14516:3084 GACGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:15426:3038 ACAAGTG +@HWI-ST575:165:C1P5BACXX:1:1101:16490:3172 GCATTCG +@HWI-ST575:165:C1P5BACXX:1:1101:16810:3009 AGTTAGC +@HWI-ST575:165:C1P5BACXX:1:1101:17555:3239 GCTATAC +@HWI-ST575:165:C1P5BACXX:1:1101:18319:3009 ATGCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:19255:3046 CAATAAC +@HWI-ST575:165:C1P5BACXX:1:1101:20168:3013 GACTCAC +@HWI-ST575:165:C1P5BACXX:1:1101:21025:3228 TATAATG +@HWI-ST575:165:C1P5BACXX:1:1101:3940:3277 TCACGGC +@HWI-ST575:165:C1P5BACXX:1:1101:5732:3431 CAATATT +@HWI-ST575:165:C1P5BACXX:1:1101:6498:3306 AGCTCAC +@HWI-ST575:165:C1P5BACXX:1:1101:6927:3383 ATACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:8668:3366 AACCCTC +@HWI-ST575:165:C1P5BACXX:1:1101:9555:3271 TCGCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:9960:3491 ATATCGC +@HWI-ST575:165:C1P5BACXX:1:1101:11371:3324 TTCGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:11999:3288 CCATATC +@HWI-ST575:165:C1P5BACXX:1:1101:12147:3289 TCCAACG +@HWI-ST575:165:C1P5BACXX:1:1101:12666:3312 TTACATG +@HWI-ST575:165:C1P5BACXX:1:1101:12701:3456 CCAATCC +@HWI-ST575:165:C1P5BACXX:1:1101:13062:3317 TTTCTCC +@HWI-ST575:165:C1P5BACXX:1:1101:13545:3337 CAACTAC +@HWI-ST575:165:C1P5BACXX:1:1101:14345:3429 CCAATAT +@HWI-ST575:165:C1P5BACXX:1:1101:14585:3422 AGATTAC +@HWI-ST575:165:C1P5BACXX:1:1101:16628:3381 TTCCCTC +@HWI-ST575:165:C1P5BACXX:1:1101:1080:3578 TACGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:1525:3749 TGCGGCC +@HWI-ST575:165:C1P5BACXX:1:1101:2431:3630 AACCCCC +@HWI-ST575:165:C1P5BACXX:1:1101:2802:3618 TGACTTT +@HWI-ST575:165:C1P5BACXX:1:1101:3064:3520 CCTGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:3610:3614 GCGCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:4225:3617 TCGAGCT +@HWI-ST575:165:C1P5BACXX:1:1101:4165:3628 ACCATAC +@HWI-ST575:165:C1P5BACXX:1:1101:4951:3655 CGACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:4878:3725 TACATAT +@HWI-ST575:165:C1P5BACXX:1:1101:5530:3596 CTCGTTT +@HWI-ST575:165:C1P5BACXX:1:1101:6120:3600 ACAACAC +@HWI-ST575:165:C1P5BACXX:1:1101:6900:3547 CAAGCAT +@HWI-ST575:165:C1P5BACXX:1:1101:7633:3566 CAACACT +@HWI-ST575:165:C1P5BACXX:1:1101:9213:3506 TCCCCCT +@HWI-ST575:165:C1P5BACXX:1:1101:10414:3701 GCAATTC +@HWI-ST575:165:C1P5BACXX:1:1101:10901:3561 GCTACCT +@HWI-ST575:165:C1P5BACXX:1:1101:10915:3580 CTGTACA +@HWI-ST575:165:C1P5BACXX:1:1101:11184:3661 CCTATTC +@HWI-ST575:165:C1P5BACXX:1:1101:11415:3524 CTAGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:12086:3581 TCCATCC +@HWI-ST575:165:C1P5BACXX:1:1101:12420:3632 ACCTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:13315:3747 TCAGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:14374:3673 CATGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:14502:3661 ACACAGC +@HWI-ST575:165:C1P5BACXX:1:1101:14952:3691 CATCATT +@HWI-ST575:165:C1P5BACXX:1:1101:15058:3596 AGGCTGA +@HWI-ST575:165:C1P5BACXX:1:1101:17467:3686 ACCAAAT +@HWI-ST575:165:C1P5BACXX:1:1101:17669:3607 AGCCCGG +@HWI-ST575:165:C1P5BACXX:1:1101:18356:3623 CCAGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:18913:3633 TCTGAAT +@HWI-ST575:165:C1P5BACXX:1:1101:20863:3620 CCTGTAA +@HWI-ST575:165:C1P5BACXX:1:1101:1773:3939 GTGACAC +@HWI-ST575:165:C1P5BACXX:1:1101:2081:3878 TGTGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:4430:3860 GAGCCTT +@HWI-ST575:165:C1P5BACXX:1:1101:6400:3801 AGTCCCC +@HWI-ST575:165:C1P5BACXX:1:1101:6719:3898 TGACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:8167:3757 AGAGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:9714:3894 CCATACC +@HWI-ST575:165:C1P5BACXX:1:1101:11290:3835 CTGCGGT +@HWI-ST575:165:C1P5BACXX:1:1101:12038:3902 GTAGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:12700:3779 CTTACTC +@HWI-ST575:165:C1P5BACXX:1:1101:12790:3907 GCTTCTT +@HWI-ST575:165:C1P5BACXX:1:1101:13831:3779 TACATAC +@HWI-ST575:165:C1P5BACXX:1:1101:14731:3871 ACGCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:14772:3935 AGCACAC +@HWI-ST575:165:C1P5BACXX:1:1101:15067:3763 GCCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:15156:3801 CTCCGTC +@HWI-ST575:165:C1P5BACXX:1:1101:16559:3892 GCCGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:17255:3956 GCTCATT +@HWI-ST575:165:C1P5BACXX:1:1101:17500:3790 GCGACGG +@HWI-ST575:165:C1P5BACXX:1:1101:18440:3926 GAGGTTT +@HWI-ST575:165:C1P5BACXX:1:1101:19111:3987 TACCACC +@HWI-ST575:165:C1P5BACXX:1:1101:19557:3888 CACGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:19920:3801 GGGCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:1580:4241 TTCAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:2986:4163 TCGAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:4437:4204 AACCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:5319:4088 GGTTCAC +@HWI-ST575:165:C1P5BACXX:1:1101:5702:4094 CTATATC +@HWI-ST575:165:C1P5BACXX:1:1101:5824:4111 CATCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:6984:4013 ACCGACT +@HWI-ST575:165:C1P5BACXX:1:1101:7890:4076 CCCACAC +@HWI-ST575:165:C1P5BACXX:1:1101:8052:4159 TTTTCGG +@HWI-ST575:165:C1P5BACXX:1:1101:8080:4229 TCAATCT +@HWI-ST575:165:C1P5BACXX:1:1101:8526:4228 CATTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:8765:4049 TTCCCCT +@HWI-ST575:165:C1P5BACXX:1:1101:9822:4042 TCTTCAT +@HWI-ST575:165:C1P5BACXX:1:1101:10184:4066 TAAAGCG +@HWI-ST575:165:C1P5BACXX:1:1101:11794:4113 CTTTATC +@HWI-ST575:165:C1P5BACXX:1:1101:12356:4148 CGTTCTC +@HWI-ST575:165:C1P5BACXX:1:1101:14554:4015 ACGTATC +@HWI-ST575:165:C1P5BACXX:1:1101:14736:4035 ATAGCAG +@HWI-ST575:165:C1P5BACXX:1:1101:15267:4200 GATACTC +@HWI-ST575:165:C1P5BACXX:1:1101:17040:4246 TGATTAC +@HWI-ST575:165:C1P5BACXX:1:1101:17659:4106 CTGGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:17880:4233 TTCAATC +@HWI-ST575:165:C1P5BACXX:1:1101:19348:4134 GATCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:1929:4262 GGCCCGC +@HWI-ST575:165:C1P5BACXX:1:1101:3262:4389 GCTTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:3996:4500 GCCAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:4255:4286 TTCTCCT +@HWI-ST575:165:C1P5BACXX:1:1101:4583:4479 CACCATC +@HWI-ST575:165:C1P5BACXX:1:1101:5886:4450 AACATCT +@HWI-ST575:165:C1P5BACXX:1:1101:6699:4365 CACGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:7054:4268 CCATTAC +@HWI-ST575:165:C1P5BACXX:1:1101:7021:4304 GAAGCTG +@HWI-ST575:165:C1P5BACXX:1:1101:7330:4272 GGCCGAC +@HWI-ST575:165:C1P5BACXX:1:1101:7985:4412 AACTCCC +@HWI-ST575:165:C1P5BACXX:1:1101:9154:4399 CGTACAC +@HWI-ST575:165:C1P5BACXX:1:1101:9397:4390 TTAGCTC +@HWI-ST575:165:C1P5BACXX:1:1101:9626:4444 CATCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:9920:4375 ATGTCAC +@HWI-ST575:165:C1P5BACXX:1:1101:9928:4484 ACACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:10347:4290 TCCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:10607:4474 ACCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:11109:4422 ATTTTTT +@HWI-ST575:165:C1P5BACXX:1:1101:11719:4366 CTTGGAC +@HWI-ST575:165:C1P5BACXX:1:1101:11803:4357 CAGTAGC +@HWI-ST575:165:C1P5BACXX:1:1101:12622:4263 ACGTCTC +@HWI-ST575:165:C1P5BACXX:1:1101:13888:4376 TGAGCCC +@HWI-ST575:165:C1P5BACXX:1:1101:14985:4327 TAAGCCC +@HWI-ST575:165:C1P5BACXX:1:1101:15294:4416 CACGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:16020:4286 CCTCTCG +@HWI-ST575:165:C1P5BACXX:1:1101:16371:4398 GGTCGTG +@HWI-ST575:165:C1P5BACXX:1:1101:17919:4354 ACCAGTG +@HWI-ST575:165:C1P5BACXX:1:1101:19347:4445 TATGACT +@HWI-ST575:165:C1P5BACXX:1:1101:19620:4333 CTACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:20453:4255 TCACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:20959:4324 ATTCATT +@HWI-ST575:165:C1P5BACXX:1:1101:20762:4415 CTTCGCT +@HWI-ST575:165:C1P5BACXX:1:1101:21156:4355 TCGACCC +@HWI-ST575:165:C1P5BACXX:1:1101:21005:4492 AGACTAC +@HWI-ST575:165:C1P5BACXX:1:1101:2208:4721 ACCACAC +@HWI-ST575:165:C1P5BACXX:1:1101:3594:4541 CCACTGC +@HWI-ST575:165:C1P5BACXX:1:1101:4453:4680 CTAACGG +@HWI-ST575:165:C1P5BACXX:1:1101:4574:4514 TACGGCC +@HWI-ST575:165:C1P5BACXX:1:1101:4512:4554 ATCCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:6091:4740 CCTGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:7755:4724 TCATGAC +@HWI-ST575:165:C1P5BACXX:1:1101:10497:4685 CGACCGC +@HWI-ST575:165:C1P5BACXX:1:1101:13319:4694 CATGTCC +@HWI-ST575:165:C1P5BACXX:1:1101:14064:4581 CTGACAC +@HWI-ST575:165:C1P5BACXX:1:1101:14735:4724 GGGAACC +@HWI-ST575:165:C1P5BACXX:1:1101:15633:4606 ATGAGTC +@HWI-ST575:165:C1P5BACXX:1:1101:16660:4702 ATTAGTC +@HWI-ST575:165:C1P5BACXX:1:1101:18593:4710 TTCCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:19241:4545 AGACGAC +@HWI-ST575:165:C1P5BACXX:1:1101:19601:4684 GCAATCT +@HWI-ST575:165:C1P5BACXX:1:1101:20709:4735 TCACTTC +@HWI-ST575:165:C1P5BACXX:1:1101:21224:4560 ACTTGAA +@HWI-ST575:165:C1P5BACXX:1:1101:2354:4948 TTACGAC +@HWI-ST575:165:C1P5BACXX:1:1101:2921:4941 CGCGCAT +@HWI-ST575:165:C1P5BACXX:1:1101:4132:4919 ACGACCC +@HWI-ST575:165:C1P5BACXX:1:1101:5510:4838 CGTATAC +@HWI-ST575:165:C1P5BACXX:1:1101:6248:4986 ACAAAGC +@HWI-ST575:165:C1P5BACXX:1:1101:6936:4836 CCCTGTC +@HWI-ST575:165:C1P5BACXX:1:1101:7709:4867 ACGTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:9439:4978 ACGTCCC +@HWI-ST575:165:C1P5BACXX:1:1101:9572:4907 TCAACAC +@HWI-ST575:165:C1P5BACXX:1:1101:9900:4892 CGTTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:9950:4989 CAGTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:10290:4901 TATGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:10794:4755 ATAGCCT +@HWI-ST575:165:C1P5BACXX:1:1101:11203:4970 ATCTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:11597:4816 CTAATAC +@HWI-ST575:165:C1P5BACXX:1:1101:11814:4971 GTTGGCT +@HWI-ST575:165:C1P5BACXX:1:1101:12815:4804 GTCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:15064:4795 TTACCGA +@HWI-ST575:165:C1P5BACXX:1:1101:15338:4874 TCCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:15739:4984 CCCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:16043:4995 CATAACG +@HWI-ST575:165:C1P5BACXX:1:1101:17608:4859 ACGCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:17879:4904 GTTAATC +@HWI-ST575:165:C1P5BACXX:1:1101:18072:4949 CTTAACT +@HWI-ST575:165:C1P5BACXX:1:1101:18453:4916 GGGACAC +@HWI-ST575:165:C1P5BACXX:1:1101:19254:4780 GCCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:19861:4798 ATTGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:19870:4937 CAACTCC +@HWI-ST575:165:C1P5BACXX:1:1101:19796:4989 ACCCGAC +@HWI-ST575:165:C1P5BACXX:1:1101:20053:4972 TATGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:20782:4908 CGACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:1189:5021 ACCGGAT +@HWI-ST575:165:C1P5BACXX:1:1101:1686:5115 TCATAGC +@HWI-ST575:165:C1P5BACXX:1:1101:2938:5127 GCCTCAC +@HWI-ST575:165:C1P5BACXX:1:1101:3405:5036 ACTGATC +@HWI-ST575:165:C1P5BACXX:1:1101:7045:5134 ATGGCCT +@HWI-ST575:165:C1P5BACXX:1:1101:7123:5216 CGCCCCT +@HWI-ST575:165:C1P5BACXX:1:1101:8358:5145 CTGCGGC +@HWI-ST575:165:C1P5BACXX:1:1101:8840:5173 TACGCCG +@HWI-ST575:165:C1P5BACXX:1:1101:9731:5160 AAGGCTC +@HWI-ST575:165:C1P5BACXX:1:1101:10830:5146 GAGCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:11032:5068 GCGCAGG +@HWI-ST575:165:C1P5BACXX:1:1101:11565:5085 GCACCTC +@HWI-ST575:165:C1P5BACXX:1:1101:12209:5017 ACACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:12070:5238 GGAGATC +@HWI-ST575:165:C1P5BACXX:1:1101:12614:5234 TTACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:12934:5066 TGTATAC +@HWI-ST575:165:C1P5BACXX:1:1101:12889:5153 GGCAGCT +@HWI-ST575:165:C1P5BACXX:1:1101:13006:5008 CCCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:13701:5054 AATCAGC +@HWI-ST575:165:C1P5BACXX:1:1101:13818:5190 CCCATCG +@HWI-ST575:165:C1P5BACXX:1:1101:14721:5211 GCCGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:15180:5074 TCTATTG +@HWI-ST575:165:C1P5BACXX:1:1101:15733:5028 TGGGTAT +@HWI-ST575:165:C1P5BACXX:1:1101:16783:5148 TACACAC +@HWI-ST575:165:C1P5BACXX:1:1101:17093:5065 CGGTCGT +@HWI-ST575:165:C1P5BACXX:1:1101:18177:5052 TCATTAC +@HWI-ST575:165:C1P5BACXX:1:1101:18454:5064 CATATGC +@HWI-ST575:165:C1P5BACXX:1:1101:19514:5155 TTCTTAT +@HWI-ST575:165:C1P5BACXX:1:1101:21070:5172 GCATATC +@HWI-ST575:165:C1P5BACXX:1:1101:1152:5446 CATACAC +@HWI-ST575:165:C1P5BACXX:1:1101:2332:5476 CACCGTA +@HWI-ST575:165:C1P5BACXX:1:1101:3549:5422 TTTCCGC +@HWI-ST575:165:C1P5BACXX:1:1101:3934:5255 TACATGG +@HWI-ST575:165:C1P5BACXX:1:1101:3779:5257 CGCGGTC +@HWI-ST575:165:C1P5BACXX:1:1101:3981:5396 TCCAGTC +@HWI-ST575:165:C1P5BACXX:1:1101:4352:5435 CTTACAC +@HWI-ST575:165:C1P5BACXX:1:1101:5757:5381 GTACATT +@HWI-ST575:165:C1P5BACXX:1:1101:7084:5290 GCATCCC +@HWI-ST575:165:C1P5BACXX:1:1101:7550:5435 CGCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:8518:5454 GACGGAC +@HWI-ST575:165:C1P5BACXX:1:1101:8740:5499 CTTAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:8810:5337 TGCACGC +@HWI-ST575:165:C1P5BACXX:1:1101:10317:5279 TGACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:11346:5362 ACAATTC +@HWI-ST575:165:C1P5BACXX:1:1101:12293:5318 AGCGACG +@HWI-ST575:165:C1P5BACXX:1:1101:12385:5426 CAAGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:12559:5359 GCGGGAC +@HWI-ST575:165:C1P5BACXX:1:1101:13619:5383 AGCGGTC +@HWI-ST575:165:C1P5BACXX:1:1101:14813:5367 TGCCCAT +@HWI-ST575:165:C1P5BACXX:1:1101:16958:5440 TCCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:17064:5269 CCTACAT +@HWI-ST575:165:C1P5BACXX:1:1101:18711:5374 TTGTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:19795:5388 GACTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:1536:5742 ACCCCCC +@HWI-ST575:165:C1P5BACXX:1:1101:3186:5524 CTTCTCC +@HWI-ST575:165:C1P5BACXX:1:1101:3099:5544 CCAATCC +@HWI-ST575:165:C1P5BACXX:1:1101:4347:5721 ATCGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:5268:5661 TAACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:6568:5658 TGCAATC +@HWI-ST575:165:C1P5BACXX:1:1101:7344:5519 CACTAGC +@HWI-ST575:165:C1P5BACXX:1:1101:8216:5553 GGTACCC +@HWI-ST575:165:C1P5BACXX:1:1101:8183:5554 CTTAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:8832:5696 AACGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:9211:5626 TACTATC +@HWI-ST575:165:C1P5BACXX:1:1101:9028:5675 TATTACT +@HWI-ST575:165:C1P5BACXX:1:1101:9789:5670 GCGTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:10507:5654 TCAAACC +@HWI-ST575:165:C1P5BACXX:1:1101:12148:5538 GACCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:12149:5686 ATCCAGC +@HWI-ST575:165:C1P5BACXX:1:1101:12546:5747 CACTTGG +@HWI-ST575:165:C1P5BACXX:1:1101:13615:5616 GCTATTC +@HWI-ST575:165:C1P5BACXX:1:1101:13537:5735 GCTTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:14584:5542 GCCGAAG +@HWI-ST575:165:C1P5BACXX:1:1101:15724:5746 TGTGGAT +@HWI-ST575:165:C1P5BACXX:1:1101:17227:5625 ACCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:17881:5507 TAACCCT +@HWI-ST575:165:C1P5BACXX:1:1101:20112:5554 CTCTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:20322:5548 ACCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:21233:5679 CCCCCGC +@HWI-ST575:165:C1P5BACXX:1:1101:1959:5920 GCAGTGT +@HWI-ST575:165:C1P5BACXX:1:1101:1924:5978 GCCTCTC +@HWI-ST575:165:C1P5BACXX:1:1101:4153:5778 CGTGAGC +@HWI-ST575:165:C1P5BACXX:1:1101:4024:5788 CGTTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:4463:5945 CTGCGAC +@HWI-ST575:165:C1P5BACXX:1:1101:6188:5778 GCGGTCG +@HWI-ST575:165:C1P5BACXX:1:1101:6867:5946 CCACAAT +@HWI-ST575:165:C1P5BACXX:1:1101:7535:5814 TGCAACG +@HWI-ST575:165:C1P5BACXX:1:1101:7794:5903 ACAATGC +@HWI-ST575:165:C1P5BACXX:1:1101:9106:5832 CTCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:9392:5986 TTGACCC +@HWI-ST575:165:C1P5BACXX:1:1101:9549:5793 CTTATAT +@HWI-ST575:165:C1P5BACXX:1:1101:10541:5867 TCGCCCT +@HWI-ST575:165:C1P5BACXX:1:1101:11397:5818 AATCTAT +@HWI-ST575:165:C1P5BACXX:1:1101:13482:5805 TAAGGCT +@HWI-ST575:165:C1P5BACXX:1:1101:13731:5797 CACTAAG +@HWI-ST575:165:C1P5BACXX:1:1101:13983:5888 TGGCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:13915:5939 CGTGGTC +@HWI-ST575:165:C1P5BACXX:1:1101:14076:5802 CTCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:14165:5883 ATGCTTC +@HWI-ST575:165:C1P5BACXX:1:1101:14716:5991 CGCCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:15153:5829 CCCACCC +@HWI-ST575:165:C1P5BACXX:1:1101:15618:5813 GGGAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:16278:5962 GCCGACC +@HWI-ST575:165:C1P5BACXX:1:1101:17404:5768 AGTCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:18508:5770 ATACGGT +@HWI-ST575:165:C1P5BACXX:1:1101:18654:5893 GTTGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:20005:5823 TCTATAC +@HWI-ST575:165:C1P5BACXX:1:1101:20461:5768 TCTTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:1693:6048 AACCCTT +@HWI-ST575:165:C1P5BACXX:1:1101:3291:6004 CCCACCC +@HWI-ST575:165:C1P5BACXX:1:1101:3937:6198 CCTTCTC +@HWI-ST575:165:C1P5BACXX:1:1101:4273:6038 CACGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:4445:6115 CTGCAGC +@HWI-ST575:165:C1P5BACXX:1:1101:4344:6153 AGTGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:5212:6077 GCTCGTC +@HWI-ST575:165:C1P5BACXX:1:1101:5854:6193 TTGACGA +@HWI-ST575:165:C1P5BACXX:1:1101:6093:6246 AAGGGCT +@HWI-ST575:165:C1P5BACXX:1:1101:8105:6158 GTCTAGC +@HWI-ST575:165:C1P5BACXX:1:1101:8253:6223 GATAGCC +@HWI-ST575:165:C1P5BACXX:1:1101:8552:6031 GCTAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:8575:6101 ACTACTA +@HWI-ST575:165:C1P5BACXX:1:1101:9569:6015 CAACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:10905:6024 GCAGCCT +@HWI-ST575:165:C1P5BACXX:1:1101:11477:6010 CCGTTGT +@HWI-ST575:165:C1P5BACXX:1:1101:12389:6154 CCACCTC +@HWI-ST575:165:C1P5BACXX:1:1101:12820:6019 ACACCCC +@HWI-ST575:165:C1P5BACXX:1:1101:13503:6047 CCGCTCA +@HWI-ST575:165:C1P5BACXX:1:1101:14071:6041 TTATGAC +@HWI-ST575:165:C1P5BACXX:1:1101:14685:6102 ACTTACC +@HWI-ST575:165:C1P5BACXX:1:1101:14930:6229 GCCCATC +@HWI-ST575:165:C1P5BACXX:1:1101:15280:6053 CCCGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:15344:6191 CAAATCC +@HWI-ST575:165:C1P5BACXX:1:1101:16410:6086 CGCTGGT +@HWI-ST575:165:C1P5BACXX:1:1101:16374:6145 CCTGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:16749:6158 CGGCTAA +@HWI-ST575:165:C1P5BACXX:1:1101:17553:6192 CACCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:17938:6081 TAAGTGC +@HWI-ST575:165:C1P5BACXX:1:1101:19315:6150 TTACCTC +@HWI-ST575:165:C1P5BACXX:1:1101:19532:6018 CACCCTC +@HWI-ST575:165:C1P5BACXX:1:1101:21261:6248 ATACATC +@HWI-ST575:165:C1P5BACXX:1:1101:1089:6262 AACATGC +@HWI-ST575:165:C1P5BACXX:1:1101:1439:6319 GCGGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:1829:6451 CGGATAT +@HWI-ST575:165:C1P5BACXX:1:1101:2484:6444 GAATGAC +@HWI-ST575:165:C1P5BACXX:1:1101:3350:6364 CATTGCG +@HWI-ST575:165:C1P5BACXX:1:1101:3699:6336 CAGGCTT +@HWI-ST575:165:C1P5BACXX:1:1101:3874:6280 AACCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:4404:6415 CCAAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:5416:6451 CAATGTT +@HWI-ST575:165:C1P5BACXX:1:1101:7066:6455 CCTAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:7488:6251 GCGAGCC +@HWI-ST575:165:C1P5BACXX:1:1101:7486:6329 TAACCAT +@HWI-ST575:165:C1P5BACXX:1:1101:7643:6450 AAAGCCT +@HWI-ST575:165:C1P5BACXX:1:1101:8454:6487 CACAGTT +@HWI-ST575:165:C1P5BACXX:1:1101:9137:6329 TGACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:9587:6277 TATTACC +@HWI-ST575:165:C1P5BACXX:1:1101:9536:6348 CAATTCC +@HWI-ST575:165:C1P5BACXX:1:1101:10054:6289 ACCTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:10173:6294 CAAAGAT +@HWI-ST575:165:C1P5BACXX:1:1101:10764:6333 ATTCGCC +@HWI-ST575:165:C1P5BACXX:1:1101:11047:6477 TATACGT +@HWI-ST575:165:C1P5BACXX:1:1101:11411:6372 AATCCGC +@HWI-ST575:165:C1P5BACXX:1:1101:11665:6448 CCCTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:12774:6457 TATATAC +@HWI-ST575:165:C1P5BACXX:1:1101:13106:6369 CAGGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:16704:6321 CCCTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:16660:6380 CACTCTT +@HWI-ST575:165:C1P5BACXX:1:1101:17964:6393 TTCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:18173:6282 CTTTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:18209:6492 TACACAC +@HWI-ST575:165:C1P5BACXX:1:1101:18546:6430 TACTTCT +@HWI-ST575:165:C1P5BACXX:1:1101:19023:6315 CGATTCC +@HWI-ST575:165:C1P5BACXX:1:1101:19100:6466 GACTCCT +@HWI-ST575:165:C1P5BACXX:1:1101:20025:6400 CTCATAT +@HWI-ST575:165:C1P5BACXX:1:1101:20043:6415 TCACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:1982:6500 TCGACAC +@HWI-ST575:165:C1P5BACXX:1:1101:1934:6651 TGACACG +@HWI-ST575:165:C1P5BACXX:1:1101:3031:6587 TCCGATC +@HWI-ST575:165:C1P5BACXX:1:1101:3659:6632 TAGGCTC +@HWI-ST575:165:C1P5BACXX:1:1101:4271:6639 GCGTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:5521:6516 ACAGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:6888:6616 GCACACT +@HWI-ST575:165:C1P5BACXX:1:1101:6970:6733 CCTAGCT +@HWI-ST575:165:C1P5BACXX:1:1101:9786:6627 ATCACAC +@HWI-ST575:165:C1P5BACXX:1:1101:10466:6616 CGCCGAT +@HWI-ST575:165:C1P5BACXX:1:1101:12094:6626 GAGGACG +@HWI-ST575:165:C1P5BACXX:1:1101:13236:6588 GCTCGGG +@HWI-ST575:165:C1P5BACXX:1:1101:13123:6721 GGTAATC +@HWI-ST575:165:C1P5BACXX:1:1101:13878:6710 CGACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:14181:6588 GGATCAG +@HWI-ST575:165:C1P5BACXX:1:1101:14627:6506 TACGACC +@HWI-ST575:165:C1P5BACXX:1:1101:15075:6709 TTTCTTC +@HWI-ST575:165:C1P5BACXX:1:1101:16741:6696 GTTACAC +@HWI-ST575:165:C1P5BACXX:1:1101:16765:6674 TCATAAC +@HWI-ST575:165:C1P5BACXX:1:1101:19358:6651 GTTGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:19561:6627 TTACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:20819:6735 TTTGCAT +@HWI-ST575:165:C1P5BACXX:1:1101:1564:6811 TACGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:1927:6913 GCTAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:2572:6857 CACCGTC +@HWI-ST575:165:C1P5BACXX:1:1101:3215:6869 TGCACAC +@HWI-ST575:165:C1P5BACXX:1:1101:3121:6916 GACATAT +@HWI-ST575:165:C1P5BACXX:1:1101:5462:6854 CACAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:6911:6752 AACAACG +@HWI-ST575:165:C1P5BACXX:1:1101:8402:6992 TCCCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:10199:6779 TTTGAGC +@HWI-ST575:165:C1P5BACXX:1:1101:11749:6891 GATTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:12769:6834 GCTGAAT +@HWI-ST575:165:C1P5BACXX:1:1101:13711:6990 GCGCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:14111:6893 GTTATGC +@HWI-ST575:165:C1P5BACXX:1:1101:14440:6994 CTGAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:15694:6869 CCGCATC +@HWI-ST575:165:C1P5BACXX:1:1101:16093:6801 ACCAGCC +@HWI-ST575:165:C1P5BACXX:1:1101:16736:6834 GACGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:16688:6982 CGAAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:17036:6768 ACCGAGC +@HWI-ST575:165:C1P5BACXX:1:1101:17542:6830 TTCCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:18093:6840 TCTATAC +@HWI-ST575:165:C1P5BACXX:1:1101:18333:6967 AACCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:19230:6988 GCGCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:20486:6800 CAGTCAC +@HWI-ST575:165:C1P5BACXX:1:1101:20297:6938 AAATCCC +@HWI-ST575:165:C1P5BACXX:1:1101:20449:6990 ACACAAT +@HWI-ST575:165:C1P5BACXX:1:1101:1103:7035 GATGTGC +@HWI-ST575:165:C1P5BACXX:1:1101:1500:7064 GAGGCTC +@HWI-ST575:165:C1P5BACXX:1:1101:1577:7179 AGATATC +@HWI-ST575:165:C1P5BACXX:1:1101:2297:7148 GTAAACG
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/k2n.txt Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,58 @@ +1 2 3 4 5 +6 7 8 9 10 +11 12 13 14 15 +16 17 18 19 20 +21 22 23 24 25 +26 27 28 29 30 +31 32 33 34 35 +36 37 38 39 40 +41 42 43 44 45 +46 47 48 49 50 +51 52 53 54 55 +56 57 58 59 60 +61 62 63 64 65 +66 67 68 69 70 +71 72 73 74 75 +76 77 78 79 80 +81 82 83 84 85 +86 87 88 90 91 +92 93 94 95 96 +97 98 99 100 101 +102 103 104 105 106 +107 108 109 110 111 +112 113 114 115 116 +117 118 119 120 121 +122 123 124 125 126 +127 128 129 130 131 +132 133 134 135 136 +137 138 139 140 141 +142 143 144 145 146 +147 148 149 150 151 +152 153 155 156 157 +158 159 160 161 162 +163 164 165 166 167 +168 169 170 171 172 +173 174 175 176 177 +178 179 180 181 182 +183 184 185 186 187 +188 189 190 191 192 +193 194 195 196 197 +198 200 201 202 203 +204 205 206 207 208 +209 210 211 212 213 +214 215 216 217 218 +219 220 221 222 223 +224 225 226 227 228 +229 230 231 232 233 +234 235 237 238 239 +240 241 242 243 244 +245 246 247 248 249 +250 251 252 253 254 +255 256 257 258 259 +260 261 262 263 264 +265 266 267 269 270 +271 272 273 274 275 +276 277 278 279 280 +281 282 283 284 285 +286 287 288 289 290 +291 292 293 294
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/read_counts.txt Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,108 @@ +RNase_P 110 187 2 +RNase_P 111 187 4 +RNase_P 112 187 2 +RNase_P 115 187 1 +RNase_P 117 187 5 +RNase_P 117 188 1 +RNase_P 119 186 1 +RNase_P 134 188 1 +RNase_P 137 187 1 +RNase_P 138 187 1 +RNase_P 152 187 1 +RNase_P 10 187 1 +RNase_P 11 187 1 +RNase_P 15 187 2 +RNase_P 30 187 2 +RNase_P 31 186 1 +RNase_P 31 188 1 +RNase_P 18 186 1 +RNase_P 18 187 1 +RNase_P 19 187 1 +RNase_P 32 187 1 +RNase_P 33 187 1 +RNase_P 50 187 2 +RNase_P 51 187 1 +RNase_P 38 187 1 +RNase_P 39 187 1 +RNase_P 55 187 3 +RNase_P 56 187 1 +RNase_P 2 187 2 +RNase_P 57 187 2 +RNase_P 71 187 2 +RNase_P 3 185 1 +RNase_P 3 186 9 +RNase_P 3 187 96 +RNase_P 3 188 1 +RNase_P 58 187 3 +RNase_P 59 187 1 +RNase_P 4 185 1 +RNase_P 4 186 32 +RNase_P 4 187 307 +RNase_P 73 187 1 +RNase_P 5 187 14 +RNase_P 6 186 2 +RNase_P 6 187 10 +RNase_P 7 187 4 +RNase_P 8 187 4 +RNase_P 90 187 2 +RNase_P 77 187 4 +RNase_P 9 187 3 +RNase_P 78 187 2 +RNase_P 92 187 2 +RNase_P 93 187 1 +RNase_P 94 187 2 +RNase_P 100 187 1 +RNase_P 97 186 1 +RNase_P 97 187 4 +RNase_P 98 187 1 +RNase_P 101 187 2 +RNase_P 102 187 1 +RNase_P 99 187 5 +RNase_P 103 187 1 +RNase_P 104 187 1 +RNase_P 105 187 1 +RNase_P 106 187 2 +RNase_P 107 186 1 +RNase_P 107 187 3 +RNase_P 121 187 2 +RNase_P 109 187 2 +RNase_P 125 187 1 +RNase_P 126 187 2 +RNase_P 128 187 3 +RNase_P 142 187 1 +RNase_P 129 187 1 +RNase_P 145 187 1 +RNase_P 147 187 1 +RNase_P 148 187 1 +RNase_P 163 187 1 +RNase_P 169 187 1 +RNase_P 20 187 1 +RNase_P 21 186 2 +RNase_P 21 187 7 +RNase_P 22 187 2 +RNase_P 23 185 1 +RNase_P 23 187 4 +RNase_P 24 187 1 +RNase_P 25 187 1 +RNase_P 40 186 1 +RNase_P 40 187 7 +RNase_P 27 186 1 +RNase_P 27 187 1 +RNase_P 41 187 1 +RNase_P 42 187 1 +RNase_P 43 187 1 +RNase_P 44 186 1 +RNase_P 44 187 1 +RNase_P 45 187 2 +RNase_P 48 186 1 +RNase_P 63 187 1 +RNase_P 64 187 1 +RNase_P 65 187 1 +RNase_P 68 187 1 +RNase_P 81 187 2 +RNase_P 82 187 2 +RNase_P 83 187 1 +RNase_P 85 187 2 +RNase_P 86 187 1 +RNase_P 89 147 1 +RNase_P 89 187 6
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/reads1.fastq Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,2684 @@ +@HWI-ST575:165:C1P5BACXX:1:1101:1666:1132 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +DHHHFGGIJEGGGHGIIJDH<CAEHGEGGEGI=FG@FDHAFBF@=FGB@GFFFF'(.; +@HWI-ST575:165:C1P5BACXX:1:1101:2239:1101 +GGATCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHDHHFHHIJJJJIJJJDDGIGIIGIJJIIIIIIECHGIIIJGIFHHEEBBCDDB?BCDD +@HWI-ST575:165:C1P5BACXX:1:1101:2455:1083 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHFD<CGGGHHIGFHD:FGCG@F>DFDFHAGCB=FCGGEGHHGGIGEEBBDCBBBBCC +@HWI-ST575:165:C1P5BACXX:1:1101:3207:1238 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FADFFAEGGIEGIEBHG@GEA@BBGHEF>F8?78B@FCGGICEGECCABA?BA@=?C? +@HWI-ST575:165:C1P5BACXX:1:1101:3405:1152 +TGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCT ++ +DFDFD2@CEB:<3)?EF;C:91?DGBF??BBF<0909B?DDB26= +@HWI-ST575:165:C1P5BACXX:1:1101:3512:1179 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGGHGHJJJJJIJJIIJIJIIHJHJJJGIIJJJJJJJJJGJHIIIIGEHGFFDDBDDDDDB?ACCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4473:1182 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FFHFHF@GGIIIDII:FFBDFHGGEHGIIBGGGGGIGICGIG;DGE>CHFFC<?B?A>A +@HWI-ST575:165:C1P5BACXX:1:1101:5162:1092 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHGHIIJJJJJJFHIIJHIJJIJJJJJJJJJIGHJJJIJIIIIIJHHGFDDDBBDDDDDDDDDDBB +@HWI-ST575:165:C1P5BACXX:1:1101:6810:1177 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHHFHHJJJJJJJJJIJIIJJGIJIJJJIIJJJJJJJJJJJJIJJJJIGHGFFDD?BD +@HWI-ST575:165:C1P5BACXX:1:1101:8268:1157 +TCGGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCCTCGGCGGATCCAA ++ +FGHHHHJJJJJIJJIJIJJJIIIJIBFHIJJIIJJJJJJIGDFFEEEDDDDDDDDDDDDDDDDDDDDDDD@@>C>C +@HWI-ST575:165:C1P5BACXX:1:1101:8740:1185 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGFHHHJJJJIJJJJJJIJIGIJIJJJJIJJGIJJJJJIIIJJJJIJHHHFDDDDDD;AB@BBDCCBD +@HWI-ST575:165:C1P5BACXX:1:1101:12414:1099 +GATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGA ++ +FFHHHHJJJJJJIJJJJJJIGIJGFGGHJJJJGEIIJJJJIIHHHIIIHHHHHHFDFFFD +@HWI-ST575:165:C1P5BACXX:1:1101:14053:1112 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHFHJJJJJJHHHIJIJJIJJJJJJJGDIIJJJJJIJJJGGJIIIJHHFFDDBBD9 +@HWI-ST575:165:C1P5BACXX:1:1101:14219:1152 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJJIHIHGIFGIIJJJJJJIIJJJJJJIJIIJFIIJIJJHHFFDDB??C9A +@HWI-ST575:165:C1P5BACXX:1:1101:15012:1211 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FDFHHHIJJJJIJJGJJIIJJJJIJJJI@EGIJJJJJJHGJJIJHIFHHFFDDDBAC<B +@HWI-ST575:165:C1P5BACXX:1:1101:15774:1231 +AAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTC ++ +FHHHHHIJJJJJJJJJJJIJJJIJJJIJJJJIIJJJJJJIJJIJIJIJHJIGJJICEHHF +@HWI-ST575:165:C1P5BACXX:1:1101:17047:1127 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHGGFIJJJJJIIE@GGIHGIIJIIIIIHHHIIJIJJJJGIIJIJIHHGFDDD@DC5 +@HWI-ST575:165:C1P5BACXX:1:1101:18461:1188 +AAATGGGGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAAT ++ +DBFFDH+<EHHIDGHIIBHI@F?DFGIIGAGG;@@BHD<B?ABBBB8;@B<5-7?>A3> +@HWI-ST575:165:C1P5BACXX:1:1101:19911:1138 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHDHHDGIHIIIIIIDGGHIICGFHIIIGIIIIIIIIIIIIHHIIHHIFFFFCC?=CC; +@HWI-ST575:165:C1P5BACXX:1:1101:1311:1500 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHHHHHIJJJIJHHBGGHGIICHGFHIJJIHHIIIEIJHIIJIHGHFFHFFDDDBB?ABBDD@CDCBBDCDDDB<? +@HWI-ST575:165:C1P5BACXX:1:1101:2110:1396 +TCTTCCGATCTCCATTCATCTTTAGAGGCTGACGGCAGAAAAAAAGCCTACGTCTTCGGATATGGC ++ +FFFHGFHHGGGICGGGGGGGIIIGIBG@FHEFCFDFGGCEH@@B<9@(;ACBBDCBCA?@BBCDBB +@HWI-ST575:165:C1P5BACXX:1:1101:2970:1430 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHHFHHIJJJJJJGEHIJIIJIEHDGIGFHIIJHHHFHHEFFCBCDE@C?BDDDDBBD +@HWI-ST575:165:C1P5BACXX:1:1101:4079:1444 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHFDHHIIHHJGJEHG:?GHHCHBDFHGIGGGHI3BBAC@FC;CDAECBHEA>B1=;;5 +@HWI-ST575:165:C1P5BACXX:1:1101:5495:1254 +ACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAA ++ +FHHHGHJJJJICFGGIJHJJJFFFHIJGHJJJGIIIIIIJIFFFDDDDDDDDDDDDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:5684:1314 +AGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTC ++ +FHHHGFHHGHIIJIJJIIJJJJJFCHHIHJIJIJCHIJJJJJIGGIGHIJJIGIHHHHHFFFDD +@HWI-ST575:165:C1P5BACXX:1:1101:6002:1450 +GCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC ++ +FHHHHHJHIIJIJJIIIIJJIGHIIJJJJJBGHGJJFGDEGEFHGIBEHHCEFFFCADDBDD@BBBB +@HWI-ST575:165:C1P5BACXX:1:1101:6163:1455 +TTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHHHHGIJIJIIIIJICHCHGIHIJIEGHHIIJGHGHFHIIJJIF4ADDDBDDDDDDDDDDB@DCCCCDCDB +@HWI-ST575:165:C1P5BACXX:1:1101:6370:1342 +GAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG ++ +DBFDHFGIB??EFH?FGDHHB?D@B@DAHGIIIGGII9@7CAFFCAHADCCH?CACD +@HWI-ST575:165:C1P5BACXX:1:1101:7500:1303 +TTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGA ++ +FHHHHGJHHJJJJJJHJIJJDGIGIIJIIJJIIJJJIJHHIJIJJIJJGIIGIIHDEHFFFFDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8186:1308 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGAAGGAAA ++ +FDACFFHIEIGIIE8CHE6BDFGEDCBBD@BCFHHHIDHIIIHBCH>=CD>9?<B=A? +@HWI-ST575:165:C1P5BACXX:1:1101:10049:1341 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGG ++ +@FF8CDFHGGBCD@?D@8(988?D48===B=7;;7@CE@D>779?DB?39< +@HWI-ST575:165:C1P5BACXX:1:1101:10737:1349 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAAGCCTACGT ++ +FHDFHHIJJJJJIIIJJGGIGIIGIJJIIJIJJJIJJJHHHFEFFFFECDDDDD<BC3<BDBBBC>95? +@HWI-ST575:165:C1P5BACXX:1:1101:11993:1308 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHADGIIIGIHIJJJEGGGGIGIIIJIJJJJJGGGGIIIIIFHHDHFDADDB?ACDBD +@HWI-ST575:165:C1P5BACXX:1:1101:12260:1420 +TGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJGIJJIIJJJIIIJJJIHGJJJEIHGGHJJJJIIGFFFDEED;;=9?CCCDB +@HWI-ST575:165:C1P5BACXX:1:1101:13041:1365 +GGGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FAHFFHGFGIJJJIJGHIIIGIJIIAHIICFGGJG@@DHHIEHFEHEDFCDDD@BB19>CBD +@HWI-ST575:165:C1P5BACXX:1:1101:13915:1290 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHGFHHJJJJIJIJJEHIHIJIJEIJIJJGGIIJIIIIJJJIEIIJJIIJHHFDDBBC??@BDBDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13944:1432 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHGHJJJIFIIJIJJHJIIJIIIIIJJJJJJJIJJJJJJJGJIJHHHFFDDDBBC(9 +@HWI-ST575:165:C1P5BACXX:1:1101:14362:1371 +ACGACGCTCTTCCGATCTTCACCACAGACG ++ +DDHDHHIBE@EFHBC;??D90??DBBGHHI +@HWI-ST575:165:C1P5BACXX:1:1101:14865:1283 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTAGAGGCTGACGGCAGGAAAAA ++ +DDF=?CCGBGHHGD8E@E8C@D@F<<DGDFEDHG@FFBGBCBDFFGCEHIFHB@5>@;? +@HWI-ST575:165:C1P5BACXX:1:1101:15341:1459 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHHHHFGIIIJJJJIIJGGFGFHHGGJIIGIGJGIJJIJJGJGIIJHHHFFDDDBAC +@HWI-ST575:165:C1P5BACXX:1:1101:15856:1385 +TAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAA ++ +FHFGDFGIJIIIGIJJJGEIGJIHIJDIHHIJJJJJIIEHHHEEDBDFCECDEEECDC +@HWI-ST575:165:C1P5BACXX:1:1101:16168:1446 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJIIJJJJJIJIHIIIJJIIJJIHIJJJIIJJJJJGEIIIHIJHHFFDDDBDC? +@HWI-ST575:165:C1P5BACXX:1:1101:16274:1448 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHGFHGHEHIGIGII;GFHIIIGIJGHIIGIGGIJGIIJJIIIIJEHHFBBD;?C5A +@HWI-ST575:165:C1P5BACXX:1:1101:17532:1255 +TGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGA ++ +EFHB?FEGCGHIIIIIFHGIIIDEHGDG:?DGG?GHIHGG@HGIHFGCHGAF=@FE +@HWI-ST575:165:C1P5BACXX:1:1101:19852:1368 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHFFHHIGIJIJIIHJJGIGIADFGGGHIJIIJJJHIJIJIJGGIJGJJHGFDDC?CC9 +@HWI-ST575:165:C1P5BACXX:1:1101:20338:1414 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAG ++ +DDCDDCAEEEEIII1)::9D>BDBDICDDI;DDDBCDEBDCEIIIDCDDDD8? +@HWI-ST575:165:C1P5BACXX:1:1101:1782:1682 +GGTCGTGCCTAGCGAAGTCA ++ +DDD1C0C+22<CEE:D0:DD +@HWI-ST575:165:C1P5BACXX:1:1101:2733:1718 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +DHFHHFGGGGIIGIIJGHIIIGHCEHHBBGGHIIIIIC<=FHEHGIIHH6BDCB@B>?A@BABDC?BB +@HWI-ST575:165:C1P5BACXX:1:1101:2796:1586 +AGGTCGTGCCTAGCAAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGA ++ +FHHHHHIJIJJJJJIJIIHIJIJJIJJJIIIJJJJIJJIJFJIGIJJGJGHJJHFFFFCDDB;?ACCBBDDDDA@< +@HWI-ST575:165:C1P5BACXX:1:1101:3075:1523 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHAHFHGGHIIJJJIJJIHIIGICHHIG@HIJJHHIJIJJIHIHIIGHEFEDDB?ACC +@HWI-ST575:165:C1P5BACXX:1:1101:4251:1515 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHFHHFIJJJJIJJJIJIHIIJJIJJJJJJJIJJGIJJJJIIGIJIIHHHFFDD=BD?B +@HWI-ST575:165:C1P5BACXX:1:1101:4481:1718 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHHHHHJJJJJJJJJIJHIJIJJIJJJJHJJJIIJJJJJIGIIJJJJHFFFADDDDDDDDDDDDDDB@ADDCBD82 +@HWI-ST575:165:C1P5BACXX:1:1101:4978:1615 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJJJJJJIJIIIIGIJJJHIJJJJIJJHHGGHGDFFEECDDDDD?BCCBDB?BDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6935:1687 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FGDDHHHIJIJIJHGGGIFE<GHEHIGIIGGGFHJJFIJIIA@DCEHBD?DDBBD@<? +@HWI-ST575:165:C1P5BACXX:1:1101:7393:1637 +TCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGGGGT ++ +FHHHHHJFHHIIEHHGGHGIGGGIHFHIIGGIJIAHIEGGBDEHGIGIGHGB@FHGE +@HWI-ST575:165:C1P5BACXX:1:1101:7744:1585 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DA:@DFFFABFIFGEFD:DDFDFFABG@?B9BB=)8=CA@)=@EEE;?E:<<<>5=AB +@HWI-ST575:165:C1P5BACXX:1:1101:7646:1624 +TTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACC ++ +FHHHHHJJJJJJJHHJJJJJJIJJJJJJJJJJJJJJJIJJJJJIHIIJJJIIGIJJIIJHFFFFEEDDDBD?;2 +@HWI-ST575:165:C1P5BACXX:1:1101:8080:1642 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHFJJJJJIJJJIIIJJJJJGJJJJIJJJJJJJJJIJJJIJJIJJGHFBAB@DCD +@HWI-ST575:165:C1P5BACXX:1:1101:8452:1710 +AAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHHHHJJJJJJJJJJJJJIJIIJIJJJJJIJJJIJJJIJJJJIJJICEHHCFEFEDDDD?BDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8575:1513 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHDHHFHGIB>GGHGAGFEEGHC>D9?@CHIIEGGIIIGGGGHBGE>EEDFDDB??CD +@HWI-ST575:165:C1P5BACXX:1:1101:8610:1649 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHGHHHJJJJJJIJJJJJJGIGJHJIJJJIIJJJJJJJJJIJIJIIEEHFFDBB=BDDDDDBDDDDDDBDDCD<82 +@HWI-ST575:165:C1P5BACXX:1:1101:9515:1701 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +DHD@@DEG@FAGHII@DE1B>FBDDFE<=FF@;AC@DHG>7?7A==@>>AA>??B8< +@HWI-ST575:165:C1P5BACXX:1:1101:9782:1735 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHGFHHJJJJIJJJJIJJAGIIHIGHGIIIJJJJJJJJGIIJJIJHHHHFFDA>@?B> +@HWI-ST575:165:C1P5BACXX:1:1101:10892:1546 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHFHHHIIIIJJGIFHIIIJIJJJGJJJJJJGJJIJJJGGIICHIJJJJJHDFFACE3?B:?BDDC?A +@HWI-ST575:165:C1P5BACXX:1:1101:10875:1631 +CGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGCAAAAAGCCTAC ++ +FFHHHHJIJIEIIJIHIIJJJJJIIIIIJIHIIJIJIIIGHIIJIJBEHFF';AE@B?ABDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11536:1555 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGAGGGCAGGAAAAAAGCCTACGT ++ +FHHHGHJJJJIIJJJJJIJIIIJGIFIJJIJJJJIIJJIGIJIGHIHHHFFDDCDBCD@BB?ACACBB +@HWI-ST575:165:C1P5BACXX:1:1101:11540:1624 +GGGGCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHHHHFFFFFDDDDDDEDDDCCDDDDBDDBCDDEDDDDDDDDDDDDDDDDBBDCCB +@HWI-ST575:165:C1P5BACXX:1:1101:12292:1617 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHGHIIIJJIJJJJIIHGIGIEIJIIEGIJJJJEIJJJIJIIJIFFEFFDDDBDDDBBB98>>CBA +@HWI-ST575:165:C1P5BACXX:1:1101:13423:1569 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJHIHIIIIIIIIIIJJJJJJJJJIJJJJIIJGJIIIJHHFFDC???CBDB@DDCDDB +@HWI-ST575:165:C1P5BACXX:1:1101:13576:1612 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHGHGHIJIJJJJJJJIIJIIJJGIJIJJIJJJGIJJJJIIJJIHHFEEDDDDBBBDCABB8?CC?>< +@HWI-ST575:165:C1P5BACXX:1:1101:14491:1526 +CGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHGFHHEHIIJIJJJ@GHAFIIJDHIGIIHHIGIIGGIIJJJJDIIJGHHFFDBB?A>? +@HWI-ST575:165:C1P5BACXX:1:1101:15109:1589 +CCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHGHDFGIHIGHIGIJGIJJGJBGGGGHHHFFDB=@DCCDDA<B +@HWI-ST575:165:C1P5BACXX:1:1101:16270:1554 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +DHDHD<EBGCGIGHGBEFGGIG<D9BF??F>@FEHIDHIJJFHGHGHF6?B39>=5>(5 +@HWI-ST575:165:C1P5BACXX:1:1101:17059:1656 +GGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTTAGAGTGGAACTCGGTAAACCCCTC ++ +FHHHHHJHGIJIIJIIJJJIJIJIJEIGHJJJIIJJJJJJJJEIICHEHIHIGGAHHHHAAEEDFFDCCEAC?BDD +@HWI-ST575:165:C1P5BACXX:1:1101:17823:1711 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHHHHFIJJJIJIIFGI=GIHJJJIGIJJJJIIGHIJJJJGJJGIJJHHHFDDDDDC59@@?BDDDDBBCBDBD@? +@HWI-ST575:165:C1P5BACXX:1:1101:18750:1719 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHHDGEHIEIBGBEEDHGGGIHGGEHIGIGGGIIJIHIEGECG@EEEEF?>C(5>,58 +@HWI-ST575:165:C1P5BACXX:1:1101:19397:1535 +TGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJIJIJBEH?FHGADDGHJIJIJJIFHIJJJIIJI?HIIJIIJFFFCAEC; +@HWI-ST575:165:C1P5BACXX:1:1101:19270:1553 +TCTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTT ++ +DHHDHHGHIIJJIEHHGIJJIGGCGGFBGHJGHIJIJGGIGHHHC>BDEED6@CACDC +@HWI-ST575:165:C1P5BACXX:1:1101:20160:1653 +GCTCTTCCGATCTGGACTCTCGG ++ +DHHHFHHIGIJJIHGGGGGIIE7 +@HWI-ST575:165:C1P5BACXX:1:1101:20348:1677 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHCCFGIIGIIJEGGHIGGIGIJJEHJIJIIGIIIGIJJJIJJIJIGGIJHFFDBC?(==@A<CCCDD +@HWI-ST575:165:C1P5BACXX:1:1101:1152:1999 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHDHFFGJJJJIGHEHEBGIIIGGHJJJIHJIHFHGIIJEEGGHCHIJGDFFDC7=CCD@BD<CCDD? +@HWI-ST575:165:C1P5BACXX:1:1101:1575:1782 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +=A<:C<@F?C@F>GECF??BDFBE39BDDABABBF>FFFCFCCEEFCEEFDCCB<;?; +@HWI-ST575:165:C1P5BACXX:1:1101:2146:1765 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHFHFHIJJJJJJJIJIIIGIGIGHIJJJJJJIIJIJJIJIJJFIJIEEHFDDD@BCDDDBBDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:2817:1860 +TTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATC ++ +BFBBFDHIIB;FHFEAHIGH;FDCHH?;FFH@;=8=C@6;D?EHA3?;?B?6>C> +@HWI-ST575:165:C1P5BACXX:1:1101:4121:1770 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHFHFFIIIIGIIJIIICHHGJJHIEGIGHIJJIGIGIHIJJEEHIJIHFFFDD7?C;AB@AACCD>B +@HWI-ST575:165:C1P5BACXX:1:1101:4015:1808 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FAAFHDFHGGGGGJJJIJCDGEHAFFHHIIIEEHADHGCE@G@HHCAEBEF>>:7=AAAB6ABCCC@? +@HWI-ST575:165:C1P5BACXX:1:1101:4671:1789 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FG>CDHGHGGEGGGIGHIBFIHHGGCEBGGGIGI@D@EHCGIBEFHF@DB@B;>B<??A +@HWI-ST575:165:C1P5BACXX:1:1101:5190:1986 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHFHHHIJJJJJIIIJJGIGGGIHGIJIIJJJGHGIIIIGGHGIJJHHHFF?BD@BD?BDBBDDDD>A +@HWI-ST575:165:C1P5BACXX:1:1101:7320:1866 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHGFHHIIJJJJJJJJJJGGIIJJJJIIJIJJIJIIGIJIIJFGHIFHHFFDDDDBDD +@HWI-ST575:165:C1P5BACXX:1:1101:7508:1798 +TCCGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHFHIJFJIIJJJJHGIHGGIJIDHIJIGIJJJJIJJJJFIIFIIHHHFFDCDDDDACD +@HWI-ST575:165:C1P5BACXX:1:1101:9647:1841 +ACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGA ++ +FHHHHHJIJJJJJJIJIJJJJGGHIJGIJJFGIIJJJIJJJJIJIJIHIJIIJJJJJDH +@HWI-ST575:165:C1P5BACXX:1:1101:11078:1760 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHFHHJIJIIJJJJGJJGIJIJJJIJJJIJJJJHIIJJJJJIJJHHHHFFDDDDBB9AABBA?CDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12091:1910 +TCACAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHHHHJIJJJJIJJJJIJJJIJJJJJJJJJIJIJJJIJJJIHHHFFFFDEEEDCDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13182:1885 +GGTCGTGCCTAGCGAAGTCATAAGCTAGTGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHGJJIJJJJJJJJJJIIIJIJJJJGGIIJJJJJJJJIJIJIIJJJHHFFD@ACCB +@HWI-ST575:165:C1P5BACXX:1:1101:13475:1836 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJGIJJJJJJJJIIJGIIJJJIJJJJJJJJJJIJIIIJIHHHFDDDBDDDAB?9?CCCDB +@HWI-ST575:165:C1P5BACXX:1:1101:14275:1813 +AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHHIJJJJJJJIJJGIIIIDGIJIGHGIIJGIIIJJIIIIJJJIJIHHFDDAB;?;?BBBCDABA +@HWI-ST575:165:C1P5BACXX:1:1101:14914:1754 +TCAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC ++ +FHHHHHJJJIIJJJJJIJJJJIJIJJIHJJIJJGGIIGIIJIJJIJJIIIJHGGFFDDDDDBDD +@HWI-ST575:165:C1P5BACXX:1:1101:15826:1814 +TCTGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHGHDHGHHIIIIGIIIJGHGIEIHGGIF>AECBB?CDDCDD@; +@HWI-ST575:165:C1P5BACXX:1:1101:16316:1995 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +DHHFFHGFFGGHHIEGGFHGIIIIJJ><?BGCGBFHGFAHAFGGG@@EHHFFDD?BC3= +@HWI-ST575:165:C1P5BACXX:1:1101:17677:1880 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHFDHHGGGIEGHIFEFBF>DGHGIIGIIIIGIHIGHIIIDDGHHHHHFFC?B8(<C9<? +@HWI-ST575:165:C1P5BACXX:1:1101:18640:1881 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +FHHFHHIJIJJJIJJJIJJFIGIIJJJJJIIJJJIJJJJJDIIIJJHGFFFDDDBBC99?828CCCB +@HWI-ST575:165:C1P5BACXX:1:1101:18890:1897 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHHIJJJJJIIIJJJIIIJJJIJJJJJJJJIJJJJJIJJHHHHFFFDDDDB@C<9>BAACDDBD +@HWI-ST575:165:C1P5BACXX:1:1101:1102:2244 +ACGACGCTCTTCCGATCTTCCTGCTAGACGTGG ++ +D=DF8D:CEFF@GG6FGD><BFF<<FFB<D67B +@HWI-ST575:165:C1P5BACXX:1:1101:2310:2103 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +DHDHFHEHEGIG<E@DA:DFDDAE@CHG<FGIIHHFGH>@CHGED>;AADBAB';5? +@HWI-ST575:165:C1P5BACXX:1:1101:3289:2146 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGC ++ +?FDFDHIIGHGIFGGGGGHGIEGHFHIGGGHGHGGC?FHI@G>FGEGGEAE +@HWI-ST575:165:C1P5BACXX:1:1101:4895:2080 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FGHGHHJJJIJJFIJHJJIJJIIIIJJGHIGIJJGIJJJJGJEIJJIIJHHF8A@?B>C +@HWI-ST575:165:C1P5BACXX:1:1101:4932:2108 +CGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +DDDDFFGEHGA=GHII@6@0DFCH>@AAAEB8@BB(>3:C@@- +@HWI-ST575:165:C1P5BACXX:1:1101:5806:2073 +ACGACGCTCTTCCGATCTGTACCATCAGACGG ++ +DHHGHAHBHIGIGDABACGF@DFD@;?>FHA: +@HWI-ST575:165:C1P5BACXX:1:1101:6214:2065 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHDHFDHIJJJJGIHGGIHGHJGEHIGIBGGIJDDHIIJJIJJJIIJGFEEDAB=ACD@ +@HWI-ST575:165:C1P5BACXX:1:1101:6143:2137 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGG ++ +DAF@DFCGDHIBG?DGIGDFEHEF>CDGD?CCFB;FA@F>GG9CHHC=?B> +@HWI-ST575:165:C1P5BACXX:1:1101:6241:2199 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DHHHHHIJJJJIHIHJIGIIJJJJJIJJIJIIJGIIEIICGHCIIJJHHGDCB8=ACD +@HWI-ST575:165:C1P5BACXX:1:1101:7894:2030 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHIIJJJJJJIJJIJEGIJIJJJJIJJIJJJJJJJIIIIJIJHHHFFCDD?ACDDDBDDDDCBB +@HWI-ST575:165:C1P5BACXX:1:1101:8072:2245 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHJJJJIJIJJJJJJEIJJJJJIHHIJJJJJJJIIJJJFIGHEEFFFDDD?AA? +@HWI-ST575:165:C1P5BACXX:1:1101:8299:2087 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHFHHDHJJJJJIIIIDGIGIJGIJIJJFIIJJIGGJJJJIIIHHHFFFFDDBD@DDDDDBDDDDCBBBDDCDB88 +@HWI-ST575:165:C1P5BACXX:1:1101:8471:2183 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHHHHHJJJJJJJJIJJIIFHGIIJJJJGIJIJJIJJIIIEIIJIJJJJJHHED;AEED==@DDDCBDBDDDD<88 +@HWI-ST575:165:C1P5BACXX:1:1101:9051:2011 +GTGCCACAGTGACGAAGTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCAGA ++ +FHGHHGJJJEHIIJJJJIJIJIJIJJJJGJGIJJIIFGIIFGGGGJJIHHHHFFFDB6?BBDBDDDDBBDDDB95< +@HWI-ST575:165:C1P5BACXX:1:1101:11298:2099 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTCTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHJJJIGIJIJHIIEGEGHIGHIIBGIJGIIIIJJIJIIJHHHDDFDDDDB?BCC +@HWI-ST575:165:C1P5BACXX:1:1101:11886:2227 +GTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHAFHGJJIIFFHIJIJGIIGHGBGEHIHFCBBDABD>?@@87A99>CC?< +@HWI-ST575:165:C1P5BACXX:1:1101:12469:2156 +GGTCTTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAGCCTACGT ++ +FHHHHGJJJJJJIGIIJIJJIJJIJJJJJJJJJJIJJJHIIIIIJJIJIHHFF@?BCCB?=ACD<=? +@HWI-ST575:165:C1P5BACXX:1:1101:13307:2211 +TTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAG ++ +FHHHHHIJJHJJJJJJJIJJJJJJDBFHIJJJJIJJJIHGGFFFFFCBACECEDEDACACC +@HWI-ST575:165:C1P5BACXX:1:1101:13967:2112 +TGTGACGAAGTCTCACTAGAAATGGTGAGAGGGGAACGCGGTAAACCCCTC ++ +DA=DFA;<@B<:AA:A@H<A<FEE?11*???)0)??8.?:4-;;C>E;:BD +@HWI-ST575:165:C1P5BACXX:1:1101:14357:2064 +TGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAAT ++ +FHHHHGIJIJJJJJJIIIIJIJJ9EFHIJGHHGGIJJJJIGIJGGGIJHGFFFD>BDDDCDDDDDDDDD@BDA@C4 +@HWI-ST575:165:C1P5BACXX:1:1101:14498:2106 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJJJJJJJJHIIIJIIJJJJJJJJJJIJJJJJGHIJJJJIHHFFDDDBD3= +@HWI-ST575:165:C1P5BACXX:1:1101:14632:2166 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHGFHHHJJJIJJJJIJJGGJIJCHIGIGHJJJJJJJJJJJIJIIIIHHHFFDDD@B?CA +@HWI-ST575:165:C1P5BACXX:1:1101:14873:2011 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHJJJJJIIJIIJHIJIJJJJJJJIJJJJJIIJJJJIJIJIJJJHHFFDDDBD= +@HWI-ST575:165:C1P5BACXX:1:1101:15054:2152 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHFHHDHIIEHIHIIGBBFCIJJIGJIIJGIGIIJHHHEHB>@DEEDEDDDDDDBBCCB +@HWI-ST575:165:C1P5BACXX:1:1101:15855:2031 +TGACCGATCCGCTTCGGCGGATCCAAAGCGG ++ +FHHGHHJJIJJJIJIDHGGHEGIHFGEEDB? +@HWI-ST575:165:C1P5BACXX:1:1101:15913:2171 +AAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +BF<AFBGFGI9HAB?G9EGIH@FEGDBF@F=BFHIIIIF@@=CF;@E56.=>><; +@HWI-ST575:165:C1P5BACXX:1:1101:16436:2126 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGG ++ +DFA@@D@FCFAGBDDHD@8?BB?F9<94.828;@8CE@G;DEC9EHEBCDC +@HWI-ST575:165:C1P5BACXX:1:1101:16535:2143 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAG ++ +DDF:CFE+2AFGCGFG<:DBFFDFEB<??DA(?8BFFFFFAACF@DE=AED9<;,=?5;2' +@HWI-ST575:165:C1P5BACXX:1:1101:16829:2249 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DHCDHHIIIDGHCGCGFBFIDFHGIIGBDDHEH=FHIIIIEAHGIIIHEFFCC?55?3 +@HWI-ST575:165:C1P5BACXX:1:1101:17455:2003 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +DH@FF@GGIICIGGGFG?FGFFIB@GHIIIGDGDHIIIGGHIIIHFCEEECA>?5<:5<= +@HWI-ST575:165:C1P5BACXX:1:1101:17878:2158 +GGGTCGTGCCTAGCGAAGTAATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHFDDH:FHGJIIEHG:G?GHAHIGIEEIHFE=GDHHIJJIIIIEHHHG<DD@BB?B39? +@HWI-ST575:165:C1P5BACXX:1:1101:18041:2200 +CAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGTGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCA ++ +FHHHHGJJJIJJJIJIIJJJJJJIJJHIIIHIHIIJHJJHJIJHJEIGIJJIIIJFF@DD=ADDDDDD>B@BB@?> +@HWI-ST575:165:C1P5BACXX:1:1101:2284:2369 +GGTCGTGCCTAGCGAAGTCATA ++ +FDCDFFGHGHEGGG<DDBHECH +@HWI-ST575:165:C1P5BACXX:1:1101:2836:2439 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGHHGHIIIJJJJJIHFGIGIGIGIIJIHHJJJJJJJJJIJJJFHEGEHFFDDDBDDDBD@BDDD@DB +@HWI-ST575:165:C1P5BACXX:1:1101:4292:2260 +CACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC ++ +FHHHGHIIIJJJJJIJJIJIIJJJIIIIHIIIJJAGHHIGGGIGEADHHGGHHFFFD@BDBDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4629:2330 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +DHFBFCGGHDIG@G@DAFG@DFDDDGIGFEGGIIJIBGICCBH=FD9DBBHBBAABD=BB8<<AC?;< +@HWI-ST575:165:C1P5BACXX:1:1101:4537:2454 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGFHHHIJIJJJJIIIJGHIHIJIJJJGHIJIJJIJJJJJEGGCHIJJHHFFDB;?A?BD@BBDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:5316:2437 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FGHHGHJIJJJJJJIJJGHIIIJIJJJJIIIIJHGIJJJIGIIJIJJJDHFFDCBBD=A +@HWI-ST575:165:C1P5BACXX:1:1101:5705:2286 +AGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG ++ +FGHHHHIJJJIGIGGJJIJJICHIJJJHJJJFJIBHBHF?HCE@BCDECEEECCCDDCCCDDDDBDDDD@ +@HWI-ST575:165:C1P5BACXX:1:1101:6181:2354 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHFHHFIJJJJIGIJJIGJIGJJIJIJJIJJJJGIIJJJIJIJIIIHHHFFDDB;?CD +@HWI-ST575:165:C1P5BACXX:1:1101:6640:2254 +GTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHHHJJJJJGIJEHGHIIIJIJJIJJIJFGEIGIJJJGGIJIIHFHFFDDB@?CCDD +@HWI-ST575:165:C1P5BACXX:1:1101:6520:2397 +AAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTC ++ +FHHHHHJJIJIJJJIIIIIJIHJJIJJJJJJJIJJHIIHIGIIJJHHHHHHFDDFFDDCDBC +@HWI-ST575:165:C1P5BACXX:1:1101:7019:2438 +ATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAGCGG ++ +FHHHFHIJJJIIJJJIJJJJJJJJIIIJJIIJJ@GIHFFFFDDDBDBDDDDDDCDDDBD +@HWI-ST575:165:C1P5BACXX:1:1101:8048:2260 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHBHHIIIJIIJGHIJGBHGIIGGJIJHIJJJJJJJIIGCHGGIIGEHHFFCDD?BCD?@@?BDC99A +@HWI-ST575:165:C1P5BACXX:1:1101:8522:2464 +TTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTC ++ +FHHHHHJJJJJJJIIJJJJJIHIIJJJIIIIJJIIJGIIGGIGEGHJGHIHJJIJJJIIG?BDEEDDDDB +@HWI-ST575:165:C1P5BACXX:1:1101:8845:2462 +GTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +D:DA;AFFHGG>EA<<;?@>>@3;;(5=B?BB<CCEC;>>@BBB?0<5@@@<B8< +@HWI-ST575:165:C1P5BACXX:1:1101:9068:2302 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHFFHHIJIJFIGFGHIIGEHGIGIIICGHGGGIJIIIFEGHIJJIFHEED<AD?AC9<?BBDDCDBBBDDDDB82 +@HWI-ST575:165:C1P5BACXX:1:1101:11110:2418 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAACAAGCCTACGTCTTCGGAT ++ +FGFHHHJJJJJJIJIIIEIJHGJIIIJIHIJIJJIJJIGEHHGJIJIJHHFDCD@BC;ABDBBDDDBBBCDABB88 +@HWI-ST575:165:C1P5BACXX:1:1101:11986:2397 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHDFHGHHJJIGHHIGHBDFGFHIIIGEIIDFGI/BCEHIIIJCEGHEEHBBDD<?;>= +@HWI-ST575:165:C1P5BACXX:1:1101:12227:2444 +ATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAAC ++ +FGHHHHJJJJJCHIIJJJIIIJJFHIIJIJJHIGIIHIIHHEHIIIIJHIJJJIHIEHGDDG=D@? +@HWI-ST575:165:C1P5BACXX:1:1101:12315:2285 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +DHHGHHJIIGHHFHHGIEIIFHIEEHIJHIJGIHAGGHIHHIDGIIEH:EDBCDBA> +@HWI-ST575:165:C1P5BACXX:1:1101:12492:2350 +TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FGHHHHJJIJBHHIJEFIIIHGIIHCHIJJJIFGIGEIGHGIIHIFHFHFFFFDCCDBDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13583:2475 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAACTGACGGCAGGAAAAAAGCCTACGT ++ +FHGHHHJJJJJJIJJIJIGIJIIJIIIJIIGIIIHGHIJIGHHHHCFEFCDDDBBBCDBDBA@CCCBB +@HWI-ST575:165:C1P5BACXX:1:1101:14469:2354 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHFHFGHIIJJGJJJGBFGIGGCFGIHAEGGIJJJGHGHHIIIJEDHHEEBDDD9?= +@HWI-ST575:165:C1P5BACXX:1:1101:14989:2278 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJJJJJGIJGHIHIJIJJJIIIJJJJJJJJJJIIJJJJJJJHFFDDDDDAB +@HWI-ST575:165:C1P5BACXX:1:1101:15188:2338 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +FHHHHHJJJJJJIIIJJHGHIJJJJJJIIIJJJJJJJJJJIJJJIJHHGFFDDDDBDCB8;?BDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15331:2257 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHJJJJJJJJIJIIFGCFFGHGHIIIJGEGHIHJJIGGIFEHGHJFFHFFDDE>C +@HWI-ST575:165:C1P5BACXX:1:1101:15849:2368 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHFHHHJIJJJJJJJJJJJIIJIJJJJHGGIJJJIIIJJIIIIHHHGFFFDDDD?B?C5 +@HWI-ST575:165:C1P5BACXX:1:1101:16152:2410 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +FHHHGHEGIIJIGJJBHBHIIJJJGGIGIJFGIHGEFHIGIIFGIJHHHFFC?BB? +@HWI-ST575:165:C1P5BACXX:1:1101:16935:2365 +AAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGGGC ++ +FFHHGHJJIIJJIIJJJJDEGHJIGEHGCHIGJJJJJJGHCHHIJIJFEDDDBDDDDDDDBDD@DBDDCCCCB959 +@HWI-ST575:165:C1P5BACXX:1:1101:17289:2412 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHHIJIIDIJJEHIDGEGIGGIJJJJJJJJHJIJJFIIJIIHHHHFFD>BB???99 +@HWI-ST575:165:C1P5BACXX:1:1101:18384:2478 +GCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCT ++ +FGHGHHFGHIJIJIJJJJJJIJDGIJJIJIJIJJJJJJJJIJJFHCHFDDCEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19068:2414 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHFHHIIIBHHIII:EHFGIEIII*?FHDHHIIFAHCHIIGIIIIIGCHHFFCC15?;?@B<<AACB? +@HWI-ST575:165:C1P5BACXX:1:1101:19461:2478 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +FHFHHHJJJJJJJJIJJJJJIGGIIJGIIJJIJJIJJJJIIIJJJJJJHHFFDDBADDABDBBDC?@ +@HWI-ST575:165:C1P5BACXX:1:1101:19502:2351 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTT ++ +DAA0<D@:ACGGCGAFA?GEFFCGF@?BB?BD?==FDF +@HWI-ST575:165:C1P5BACXX:1:1101:19741:2372 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FGCFHHJJJJIIIIHIIFHIHCGHCHIIIIJJJHGJJJIIEHJIHGGIHHFFDDBACC< +@HWI-ST575:165:C1P5BACXX:1:1101:19565:2447 +AAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACC ++ +FGHFHHJJJJJHIIJIJJJIIIJJJJJJJJJJJJHJJJJJIJJJJJJJJJJGHHGFDEFDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20585:2479 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHDHHHIJIJIJJJJJIIJIICFDHIJJIJJIGDHIJJJJIJIIIJIFIJHHFD@>CD 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+GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHGHHFGIJIFIHJIIIIHIHIIGIJIIGIIJJIIIIJIJJJJIGGFHGHFCCD;ACCD?B<?A@?@BBCBCB982 +@HWI-ST575:165:C1P5BACXX:1:1101:7199:2618 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHFHFHGGIJJJ@FFHGFGIHIIGGECFGHIJJIIGGHAFADEHGHHHHFFD>B=?C +@HWI-ST575:165:C1P5BACXX:1:1101:10015:2714 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHDHGHJIJJJJJJHIJJJIIIJIJJJIGJIJJJJJJJJJJJJJJJHHHFFACDDDD(< +@HWI-ST575:165:C1P5BACXX:1:1101:10105:2714 +GTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAG ++ +FHHGHHJJIJGIIFGIIIIIJJJHIIJJJJHGIIGH@BHIJIJHIJIEHFFECEC?8 +@HWI-ST575:165:C1P5BACXX:1:1101:11341:2736 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHGHHIJJJIJHIIIIIIIHGJHJJJJ2BFIIJIIJJJIIIIIIIDIHHFFDDBBDDBDDBACCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11611:2512 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGGGAAAAAAGCCTACGT ++ +FHHHHHEHJJJIJJJIJJIIJGIIJJJJIIIJJJGIIJJJJIJIGHGGFFFDD98AAA@7?ACDC@A +@HWI-ST575:165:C1P5BACXX:1:1101:11611:2576 +AGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCG ++ +FHHFHHJJJJGGIJJJDIGIIIIJGHIGGIJIIED?GHIDGHGIIGIJIGGHFHEEDFFD +@HWI-ST575:165:C1P5BACXX:1:1101:11522:2633 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHIIIJIJJJJJJIJGIGIHJJJJIIJJJIIIJJJJIJJIJIIHHHFFDDBDCCDD@<ACDCBB +@HWI-ST575:165:C1P5BACXX:1:1101:12762:2599 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHFHFDHHGGHIIGIIIIHEHAGFEGIJGIJIGIJJJIGIIIGIGIHHEDFDDBDBD +@HWI-ST575:165:C1P5BACXX:1:1101:13122:2666 +TGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAAC ++ +FHHHHHIJJJJIJJJGIIIIHIJJJJJJHIJJJIGHHIIJIJJIGGIJJIJFG>EHIGHHEHH +@HWI-ST575:165:C1P5BACXX:1:1101:13331:2504 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHGHIJIJIIIIIJJJIIHGIHIJJJJJJIIJIGHIHIHIJIGIDHHFFFDDB@CCBD?,<CDDBB +@HWI-ST575:165:C1P5BACXX:1:1101:13328:2614 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FGHHHHJJJJJJJIJGIIHIJGIIIIIGHHHHFF@DEEEEEECDDDDDDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13944:2746 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHFHHFIIJJJJJJIJJGIIIIJIJJJJJJJJIHIJJJJIIJIIHHHHFFDDDDBDC< +@HWI-ST575:165:C1P5BACXX:1:1101:14544:2676 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHIJJJJJJJJJJJFHJJJFIIIIGGIJJJJIJJJJIIJIIHHHHFFDDDD?B>C +@HWI-ST575:165:C1P5BACXX:1:1101:16211:2706 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHAHHE1AEHIIIHIIHIGHIIIGIIGHGHGIGGGIIGIHGGIIIGGFHF:AD;ACCD +@HWI-ST575:165:C1P5BACXX:1:1101:17293:2515 +TGTTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHDHHHIJJIJJJJIHJJJJIIJIIIJGIIIJJJHGIJJJJIIIGIJIJIJFFBF?A6;;?@BDDDDBD +@HWI-ST575:165:C1P5BACXX:1:1101:17341:2572 +CGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +EHFHGFGBHHIEIIB<?CFHGHIGFBFE;DD@DFGBHIIIGGGEIGGE<BD;>>>@@B?ACCD@ +@HWI-ST575:165:C1P5BACXX:1:1101:17813:2676 +TTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTT ++ +DH?FD>4EBE<FHFEC3A:FHIAFGBHGHBBD8@6?B;7@CAGG>EEBC/5=??@ +@HWI-ST575:165:C1P5BACXX:1:1101:18322:2589 +TCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGA ++ +FDFHBHGIJIIIIIGHGHAFHIGIJIGIGGGHIJJJIJGHIGIEIIEGHEAFHGIAE4CC@7AEEE +@HWI-ST575:165:C1P5BACXX:1:1101:18563:2613 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHIJJJIIJJJHIJJJIGIJGIJIJJJJJJIJIJJIJIIHIIHHHHFFDDD@?39 +@HWI-ST575:165:C1P5BACXX:1:1101:19030:2538 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +DFF<<@CF?B@BD:?GFA89BBFF???BB=F@AB@D@AEFE>=AED>67399;;@8<A99 +@HWI-ST575:165:C1P5BACXX:1:1101:19375:2502 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHFHFFGIIIHGIIHGGGHIH@FFHGIIFEIJJGIIJJJIIIJIJIGHGFFDDDB??C?B@ABCC?BDADDDD388 +@HWI-ST575:165:C1P5BACXX:1:1101:19630:2588 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ 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+GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHIJJJJIJJJIJJJJJJJIJJJIJIJJJJIJJJHHHGGHFFFFEDDD>BD@BDD +@HWI-ST575:165:C1P5BACXX:1:1101:4804:2998 +GTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTAC ++ +FHGHHHJJJJJJJJJJGIEIJIIJJJJJIJJJJJIIJJJIJIJIIGHIJHHFFCEEE?@:?BCDD +@HWI-ST575:165:C1P5BACXX:1:1101:5202:2792 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHGHGEIIIGGIGFGDFGEHHEHGGIJGIII@CGEEHHHFDB?DEEDB?B88<AC9AB +@HWI-ST575:165:C1P5BACXX:1:1101:5939:2936 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHGHHHJJIJJJJJJJIJIIHIJIJJJJGDHIJJJIJJJIGGIIHGIGHHFFDBDBC?ABD@DDDCDDBDBDBB<2 +@HWI-ST575:165:C1P5BACXX:1:1101:6091:2918 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHDGHHIJIJJJGIIGIGIJHIJIIJJJFIJJJJIGIJJJGIGBEEEHFFDDDDBDD9A?>?ACCDD@ +@HWI-ST575:165:C1P5BACXX:1:1101:6392:2828 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHFFIIJJIJJJHIIJIIIJJIJJJIIJJJJJJIJJJJIJJJHHHHFFDDDD?BDD +@HWI-ST575:165:C1P5BACXX:1:1101:7180:2991 +TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +DHGFHHJJJJIIJJJHJJIIIJJJIJBHJJIIJJJJDIIJIIJJIHHHEFDDDDD?BDDDDDDDDB +@HWI-ST575:165:C1P5BACXX:1:1101:7419:2920 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHHHHHIJJJJIJJIIIIJIIJJIJIJJJIIIJGIJJIJIIJIIGHIHHHFDDD@BDDBD?BBDDDBBACBCD<82 +@HWI-ST575:165:C1P5BACXX:1:1101:7772:2979 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGAAAAAAAGCCTACGT ++ +FHHDHHHJJJJJJJJJJJJJJIJJJJJJIIGJJIIJJJJJJIFGIIHFGHFDDDBDDDDDDD<ACDCDB +@HWI-ST575:165:C1P5BACXX:1:1101:8507:2811 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +FHGFHHJJIJIJJGJHIJGIJGHIGGIJAGIJHHHHHFFCDFFDCDCBDDDDDBD@B +@HWI-ST575:165:C1P5BACXX:1:1101:8832:2869 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHHHHHHJJJIJIIIIJJHHIIIIIJGGHGIJJJGGIIJJJJIJHIIHJJHHFFDDE9 +@HWI-ST575:165:C1P5BACXX:1:1101:9485:2898 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHFIJJJJJIIIJIIJIIGIGIIJJIIIJJJJJJJIJIIIGIJIJHHF>CDBBD;ABBD?CACBA +@HWI-ST575:165:C1P5BACXX:1:1101:9990:2821 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHGHJJJJJJJJHIJIIJIJJIJIJIHIJJJJJJJJJIGGIIIJGHGFFDDDDDD(< +@HWI-ST575:165:C1P5BACXX:1:1101:10328:2966 +GTGCCTAGCGAAGTCATAAGCTAGGGTAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +FGHHGHJJJJJJJHHIIJIIJJJIIJBFHGHIJJGIGGIGIJJJJJHGFFDDEEDD=@BDDCB +@HWI-ST575:165:C1P5BACXX:1:1101:11508:2796 +GTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJJIJJJIIIJIJIJJJIJGIJJJHIIJIIIJIJBEHHFDDED(;@@=<CDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11726:2972 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGFAHF@=EHBDFB??DFGGGHG99DHCGDDGHH;4=FHICEA@EGEHB6=AC@;=>;9=8((4:?>8 +@HWI-ST575:165:C1P5BACXX:1:1101:12044:2834 +TTATCCTTGAAAGTGCCATAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGAC ++ +FHHHHHJJJJJJJHIJJIJJJJJIJJJJJIJJJBFHGIJJJJJIGIIJJJHHJE==@BHHHBDFFFDDDDDB; +@HWI-ST575:165:C1P5BACXX:1:1101:12169:2963 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHIJJJIJJJJGIJGGJGGGIIJEGHGHHFBDFFEEEEDEDDDDCDDDDDDBDCCD +@HWI-ST575:165:C1P5BACXX:1:1101:13168:2760 +TTGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJJJJJJJJJIIIIJIJJJJJJIJJCGBFGIJJIJIGGGHIIIHHDCDDBDDB +@HWI-ST575:165:C1P5BACXX:1:1101:13484:2768 +CCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATACTT ++ +DHHHHHHIIDIGIIIGIIJJIJJGIJIJBGDHIGHIHIIGJHHHHDDFFDED@>A>BDD +@HWI-ST575:165:C1P5BACXX:1:1101:13399:2774 +TAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG ++ +FHHHHHJJJJIJJJJIGJJJIFIJJJIJJJJJJGFGHJJIHHHHDDDFC@CEEACCCC>ACCDDDD@CCCD +@HWI-ST575:165:C1P5BACXX:1:1101:13467:2781 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGGHGDHIGIIICHGBHIIJIIIEGJIJFHIJJIIJIHHIHIFIGGIHBHFDD@7ACCDDB2<A@?>A +@HWI-ST575:165:C1P5BACXX:1:1101:13716:2837 +GATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAA ++ +FGHHHHJJJIIJIIJJIIJIIJJJGIJJJJJJHGJJJJIJIFIHHGHGGGIJIIGHICHGGACC?E +@HWI-ST575:165:C1P5BACXX:1:1101:14400:2843 +AGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAG ++ +FHHHHHJJJJJJIIIJIIJJIJGGJJJJJJJJJIGGGGIJHHHHB@DDFEEEEEECC@C +@HWI-ST575:165:C1P5BACXX:1:1101:14816:2802 +TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHHHHJJJJJJIJJJIIJJJIJHJJJJJJJJJJIJJIIJJJJGIJHHHHFFFFEDC;=;@CDD +@HWI-ST575:165:C1P5BACXX:1:1101:15055:2926 +GTCGTGCCTAGCGAAGTTATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +AFDA<DAADFHGGBHIBD@D>D@GH@B@?BDHGGI8=B4=@2@C62CB<<<9>=?; +@HWI-ST575:165:C1P5BACXX:1:1101:17639:2908 +TGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG ++ +FHHHHHIJJJJIJCIHGIGEIGIHIIJJJJHGIJIIJJJIHHHHEHHFEEFFFCEEE;@?BCCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20319:2983 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHDFFHJFGHHIGIIIIIJIIGHGIIIJJJIJJIJJJJIJIIJJJJIJJJHFFC?ACE;@B?BCDDDDDDDDB>88 +@HWI-ST575:165:C1P5BACXX:1:1101:20783:2769 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHFHH@GIJIFIGJEGGHIJGEGHEGGGEHHHFFFEEEDEEEDDDDDDDDDDDDDDA9? +@HWI-ST575:165:C1P5BACXX:1:1101:1673:3017 +GCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTA ++ +?FHFD?EGHHE?BFHG9AFDBHEHHE>;)?AH@GG9EI@2.;??==?<@AC;;1(;(5 +@HWI-ST575:165:C1P5BACXX:1:1101:2153:3048 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCA ++ +DA<?DF>BE>BF<E@F8:**?FF>*90?@8?7 +@HWI-ST575:165:C1P5BACXX:1:1101:4715:3184 +AGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG ++ +FHHGHGBHJFGHGGJIGHCHGDHGIICHGIIIJIFFDGGHHEIIIJGIJGGHCEBDEFF +@HWI-ST575:165:C1P5BACXX:1:1101:4796:3234 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FGFHGFFGIJIJIIGAGFHGGIHGGEEG;DFIJIGHJJIIGHIHIIDHEEF<AB;?CDD +@HWI-ST575:165:C1P5BACXX:1:1101:5104:3008 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHFHHFGHHGBHGIHIGDGCGFIBEGIGIJGIGGFHIIAGGHHDHFDE?CB;@B<AC5?B +@HWI-ST575:165:C1P5BACXX:1:1101:5272:3008 +ACGGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGAGGGCAGGAAAA ++ +FHGHHFHJJIJJJIJJJFHIGHIIJIIJJJIJIJGHGHGJJIIIHHHHFDDECDCDBBDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5594:3119 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAG ++ +DF@:DC@;D<DD386?;-=FEEEC;==766=C8 +@HWI-ST575:165:C1P5BACXX:1:1101:6927:3052 +GGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGG ++ +DHGDHHFEGICFHEHDHEFHIIDIJADFHAFGIB;CHA3=CDEFFAA=ABD@> +@HWI-ST575:165:C1P5BACXX:1:1101:7098:3130 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJJJJJJGGIIIJIJJJJIIJJJIJJJJJJIIIIIHGHHFDDDD?BDDBB;?BDDDDB +@HWI-ST575:165:C1P5BACXX:1:1101:7842:3018 +TAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCG ++ +FGHGHFGHJJJIJIGHJJJJIIIIIIJJJJJIJJGIEFGGGIGIC9?BGGFHA=;EH>BDDDB +@HWI-ST575:165:C1P5BACXX:1:1101:8316:3056 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHFHHIJJJIJJJJIJJBFFFIJFIJIIJJJIJIFIIIGIJIJJGHHHHFDDDD@BCCADD8AACDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8582:3236 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHAHHHHHGJJJEHFHGFFDHGGIIIIEGHIIJGGIDGHJGGIIIHGHHEF</3;;:C +@HWI-ST575:165:C1P5BACXX:1:1101:10343:3076 +AGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC ++ +FHFHHHJJJIGIJJJIJJJJIJJJJJJJJJJJJIJIIJJGIIJJIIJJIJJIHHFHFFFDDDBDDDDBBB +@HWI-ST575:165:C1P5BACXX:1:1101:10433:3184 +CCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHGHHHJJIGIGIJIIGJIIIJFJFD<CEEDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10787:3078 +GGTCGTGCCTAGTGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DHFFFHJJJIGE*?BAGHGHCGIAHHIGIJGEIGHIGIIJJGGFCHIIHJGH8A=A@3 +@HWI-ST575:165:C1P5BACXX:1:1101:10951:3116 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAC ++ +FHBHDFHIIIIIFIIGIGHIIIIEGGIIHFGIGGIFHIGIIGIGIGIHGHF8<B@BC( +@HWI-ST575:165:C1P5BACXX:1:1101:11196:3016 +CCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGAC ++ +FHHHHHFHIJJJJJGGGJIIJJJJJGGIJHHIIGJHHIIEHHIJIGGAGHFHFFFDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11257:3175 +ATCTGGCGTGCGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJJIJJIIIJJJJFIJJIJJIJIJJBHIJIJGGHHHFFFFEFDCCABDDAC@DDDDD2<CAB +@HWI-ST575:165:C1P5BACXX:1:1101:12061:3148 +ACACCTCTTCCGATCTCAGTCACTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGG ++ +FGGHGHJJJJJJJJJIJJIHGGJJJIFEHIIIIGIGIIJJJIJJC@EE6@DEEDBADDA=@BBDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12768:3208 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHIJJJIJIIJJJIIJJIIGIJJJJIJJJIIJJJJJJJJJJJIHHHFDDDBBCCB +@HWI-ST575:165:C1P5BACXX:1:1101:14168:3032 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +FHHFHHHGIJJIJJJ@FGGIJIIIEIIGHGIJIIHHIGGGGGCHIHFBH>D>CDD1? +@HWI-ST575:165:C1P5BACXX:1:1101:14033:3072 +TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATAT ++ +D?@8CFGFEGBFHEHFHIIIHFHGICEEHIIFHHHIIGHHIIIHGHEFFFCC??A?A8?BBC>CBCB9?C15885+ +@HWI-ST575:165:C1P5BACXX:1:1101:14598:3012 +CCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTC ++ +FFHFFHFEEHDIGG;CCCAFHBFC9CBGCAHG@E6@@1BBCEG;=E;??<?B +@HWI-ST575:165:C1P5BACXX:1:1101:14516:3084 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHFFHIIJIJIIGDGHEHGHJFIGGFFCBHHHIIJIFIGGIJHHGGDFDDDDBBD9? +@HWI-ST575:165:C1P5BACXX:1:1101:15426:3038 +TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +EHHHDHJJJJIIIIIHIIIHGIFIJJJIJJIIIHGGIIIJJJJJJJJIJHF>?@CC +@HWI-ST575:165:C1P5BACXX:1:1101:16490:3172 +GTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJIJJJJGIJIJIGIJIIIIJJJJJJJIJJJJJGIJJHHAHIHHFDDADDC==@9?CDDBB +@HWI-ST575:165:C1P5BACXX:1:1101:16810:3009 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +FHHHHHJIJJJJJIHIJCGIIJJIJIJJJJJJJIJJJJJJGGIIIHHHHFDDDD@DD<2=;<BDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17555:3239 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHIJJJJJIIJIIJIJJHJJJIIEHIIJIJHJJJJJIIJJJJHHHHFFCDD@B39ABBBBDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18319:3009 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAG ++ +FHHHHHJIGIJJIJGEGGHIIIJJJJIIJIJJIHIIIJJIEGJJJJIJHHFDDD?BC;5== +@HWI-ST575:165:C1P5BACXX:1:1101:19255:3046 +ACGCGCTCTTCCGATCTCACAGACGACG ++ +EHFFHHGIJIIJJJJIJIGIJIIJGHIA +@HWI-ST575:165:C1P5BACXX:1:1101:20168:3013 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHIJJJJJJJJHIJJJIIIJJIJJIIJJJJJJIJJIIGHIJIIGIJJGFFD=AC3= +@HWI-ST575:165:C1P5BACXX:1:1101:21025:3228 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +DFH=<DFFHEGEIBGHGIIEGEDEHBGCFFGHIIIIFHIGIEGIIIGGFIGEAD@;@A=; +@HWI-ST575:165:C1P5BACXX:1:1101:3940:3277 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJJJJIJHIIHIJGIJJJHHHHFFFFEEEEDEDDDDDDDDDDDB??CCDDBDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5732:3431 +TAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHHHHFIJJJJJJGIJIJJJJJJJIJBGFDDDCDDCDDBD +@HWI-ST575:165:C1P5BACXX:1:1101:6498:3306 +TGGTCATGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHJJJJJJJJJIJIIJIIJJJJJJJJJJJJJIIIIJJJIIJJJJIJJIHHFDFCC +@HWI-ST575:165:C1P5BACXX:1:1101:6927:3383 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHIJJJJJJJHIJIJJIJJJJJJIIIJJJJJJJJJJFIJIGECHHFFCDD?ADD +@HWI-ST575:165:C1P5BACXX:1:1101:8668:3366 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGHHHHJJJJJIJJHHIIIGIIJJJIIIIIJIJJJIGJJIHGIJIIGHEFFDCD;;AC@;=?BCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9555:3271 +ACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC ++ +FHGHFHHHIJJIHIIIIIGIIEGIDFGCFFCHGGGJJIJIJJGGH<DH;;ADBEDCBBB=BB@@ +@HWI-ST575:165:C1P5BACXX:1:1101:9960:3491 +GGGTCGTGCCTAGCGAAGTCCTAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +FGDFDHGHIGGIGGIIIIEFHIGIGGJI@D=ACEEBDFFFDCCCAD??C@D=@@D<A +@HWI-ST575:165:C1P5BACXX:1:1101:11371:3324 +AGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAA ++ +FHHHHHJJJJJJJJJJJHIJJJJJJFHIIJJGIJIGJFIJIJJJGEGHHHFFFD8?BBDBDDDDDBDDBBB><<>A +@HWI-ST575:165:C1P5BACXX:1:1101:11999:3288 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCTGGAAAA ++ +FHHFHHHIIIGDIIJEIGDEIEGIGGIGIGJJFG=FHIJFGHIJJJEEHHFD/;@=C>A +@HWI-ST575:165:C1P5BACXX:1:1101:12147:3289 +TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHHHHJJJJJJJJJJHIJJJFIIJJIJJJJIJIGIIGJJJJJHHFHFFFFEEDCDDDDDDDCB +@HWI-ST575:165:C1P5BACXX:1:1101:12666:3312 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTA ++ +FGFHFHJJJIJJJJJJIIJIHIIHIIJJJJJIJJIIJJJJIJEIIGIJJHHBDD?BCDBDB5?CD +@HWI-ST575:165:C1P5BACXX:1:1101:12701:3456 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +FHDHHHIIIJJJIIIJJGGGGGIGIJJJIJEHIGHIIIIIIJIJJFGHHFFDDB=ACDDDD?BDCC@ +@HWI-ST575:165:C1P5BACXX:1:1101:13062:3317 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +DFC@DF<@?CGGEH:DDBFHGIIG@DHGGBFHIIIIIIIGGHHEGIEHFDFC?;;;? +@HWI-ST575:165:C1P5BACXX:1:1101:13545:3337 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJIIJJIJJJJGHIIGIHIJGIGIIIJJEIJJIIJJJJHJIJHGFFDD@BDDB +@HWI-ST575:165:C1P5BACXX:1:1101:14345:3429 +CTGGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHHHHHJJJJJJIGJHIGGIHHIJJJJJIJJIJJJJHIJJJIJJGJJJJJJHHFFDD?A? +@HWI-ST575:165:C1P5BACXX:1:1101:14585:3422 +AGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG ++ +FHHHHGIJJIJIIIIJJIJJJJIEGHJJIJJJIJIJJIJJIIHIIJIJIIJJJHHHHHF +@HWI-ST575:165:C1P5BACXX:1:1101:16628:3381 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJIJJJJJJEGIGIGIIJIJJIJJJJIJIJJJJFIIIIJJJHGHFFDDDBBA<9=69ACDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:1080:3578 +GGTCNTGCCTAGCGAAGTCANAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGANAAAANCCTACGTC ++ +FGFH#2>EHIHIIHGHHHII#0?DHGIIIIIIGHIBHGCG>EIIJF@ADD=ABDB#,5??#,5<<?>AA +@HWI-ST575:165:C1P5BACXX:1:1101:1525:3749 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTGGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +DHFFFHHHJHGGHIHGHHIIHIEEIJIIGHHCHHIEEE)?ADDDDEECDB?B=??ACCBBBDDDDC@B +@HWI-ST575:165:C1P5BACXX:1:1101:2431:3630 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHGCFHHIIJJIIIJIGH?FHGIJGIGH>FFAD@CCCEEEEHFFBFAC@ADDD?D@BD +@HWI-ST575:165:C1P5BACXX:1:1101:2802:3618 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHGHJJJJIJIJIIIHIGIGIIIIJIIJIIJJJIIJJIGIGIIJJJGJHGFDBEEDDDBDDDDDBB +@HWI-ST575:165:C1P5BACXX:1:1101:3064:3520 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHFFIJJIIIIIGJEHIIJEIFHIJHIGIHGHJIJJ<FHIIIIHGGGHFDDDBDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:3610:3614 +ACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCAACAGTGAC ++ +FGHGDFHJFGBHJFFFEEB?FHIJI@FHGAFHJHCAEAE?E@;@?DC7;>;>CCCCD;55;:3 +@HWI-ST575:165:C1P5BACXX:1:1101:4225:3617 +GAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG ++ +DDBHHHIGEGEAHIGIJJIIIHFHHGGGFHGGGA@;5AEEAADDBBCCC@CCECC>>>C@@C:C9<:A:@8 +@HWI-ST575:165:C1P5BACXX:1:1101:4165:3628 +TGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHHGJJJJJJJIIJJ1CFHIJGGJJIJIJIJIGIJJIJJJJJJJIJHIGEFEBCE3>B +@HWI-ST575:165:C1P5BACXX:1:1101:4951:3655 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGG ++ +DDHDFFEGHIDHEHFEG;FIEHGE@GGIEHIGGI3CGEGG>9=CEEEH>>B>;@ +@HWI-ST575:165:C1P5BACXX:1:1101:4878:3725 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHIJJJJJJJJHIJJJIIJIJJJJJIJJJJHIGIIJJIIIJJIIJJJHHDDDEED +@HWI-ST575:165:C1P5BACXX:1:1101:5530:3596 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FGDFHHIJJJGIIIJHEHIIIJJJJIIIJJJJJJJJJJIGJIIIJJJIIHBD<<B?A3? +@HWI-ST575:165:C1P5BACXX:1:1101:6120:3600 +GATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGA ++ +DAHHGHGHIJJJHGGHIJIJGGIIFHIJJJGIIJIIGDACDFFDGGGEGGHGHGEHG:@G +@HWI-ST575:165:C1P5BACXX:1:1101:6900:3547 +TTCCGATCTTAACGACAGGCTGACGGCGGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FGFHHHIJJJJJHIFIJJJJJIJJJIJJJC@BDDDD;ADDD<@BDDDCDDDBBCCDD +@HWI-ST575:165:C1P5BACXX:1:1101:7633:3566 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +FHF?FHIIGIJJHHAEBFGIGGGIJIIGFBGIJJJJEFGBB@>AFCCHIHHB<A(> +@HWI-ST575:165:C1P5BACXX:1:1101:9213:3506 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAACA ++ +FHFHHHIGGIIJIIDHGHIIGIIIFIGFEDGGIIIIJIIJIIIJADGHGGFE>C@BC(; +@HWI-ST575:165:C1P5BACXX:1:1101:10414:3701 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHFFHGIIJJJJJJHIJDFIHIGGIJGHIJIIIHHJIIIIIGHIIJHEHIHHFD?A(=ADDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10901:3561 +TCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCA ++ +FHHHHHJJJJIJIJFFGCIHIGGIJIAEGHF@GHIJIGGGGGFGBHBDADBDDBBD@DDDBBBDA +@HWI-ST575:165:C1P5BACXX:1:1101:10915:3580 +AAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTC ++ +FHHGHHIHIJJIIJJIJJIJJFHIJJJJIJIGGHIIJIJJJIJIHHHHGHFFFFFEC?BDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11184:3661 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +BC<<DF:AEFGIBG?GD:DDFFDGEF@?BFBD<?=B<B8=C78CAC6@AA>/'31;; +@HWI-ST575:165:C1P5BACXX:1:1101:11415:3524 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHGHHHGIJIJIJJJHGIHIGIIJIGIGIJIJGIHIGHIGIIIJIJHHHFFDCBD?AC?B +@HWI-ST575:165:C1P5BACXX:1:1101:12086:3581 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJJJJJJJJIIJIJJHJJJJJJJJJJEHJJIIGJJJJJHHHFFDDB?@>CB +@HWI-ST575:165:C1P5BACXX:1:1101:12420:3632 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTAGAGGCTGACGGCAG ++ +FD;FDFEHIIII@GFHI;GGDHGB@FEHIIFEHHIIEEGA4@FFC;CHEDBB +@HWI-ST575:165:C1P5BACXX:1:1101:13315:3747 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCT ++ +ADADD<@+<ACE?E<DD?*?BDDEIDADDI;DDCAD +@HWI-ST575:165:C1P5BACXX:1:1101:14374:3673 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +FHHHGHIJJJJIJJGGIGHIGIJHIJJJGIIIJIJJJJJJGIJJJJJJHHFFDD@?C3=?BDDCDDB +@HWI-ST575:165:C1P5BACXX:1:1101:14502:3661 +GGGTCGTGCCTAGCGAAGTAATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHFDFBGGGGHIJJAGHBFGEIIIIFHGHEH?H>GIHHFHGDFFFFECDDD?BB8?(+ +@HWI-ST575:165:C1P5BACXX:1:1101:14952:3691 +CCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGAC ++ +FFHHHHJIJGHGIIJJJFHHJGHEHHIIJJJGEGGDGGAG?FHGGIGIIJIIJJIIH=EDFCDCDD>?B +@HWI-ST575:165:C1P5BACXX:1:1101:15058:3596 +CGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG ++ +FHHHHHJIIJJJJJIGJJJJJJJJGIJJJJJFGJJJIJDHHHHHHFDEFFFEEEEEEBCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17467:3686 +TTAGAGGCTGACGGCAGGAAAAAATCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCA ++ +FHHHHHJJJIIIIIJJJIEGIIIJGHIJGJJIGEIGIIIJJHHHHGFFFBDCAAEEC>@CCCCCDD +@HWI-ST575:165:C1P5BACXX:1:1101:17669:3607 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +FHFHHHJJJJJJIIHIJFGIHIJGJJJJJJJJJIJJJJIHGHHFFFFFDDDDDD@BC99@78ACDCD +@HWI-ST575:165:C1P5BACXX:1:1101:18356:3623 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJIIJJJJJJJJGHGGIJHJJJJJJJIJIJJJJJJIIFIJJIIHHFFDDBDC=A??ABCDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:18913:3633 +ATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHHHFHIJJJJJJJHIIJJJJJJJJJIIJJJJJJJJHHFFFDDDDDDDD/<BDCACCCDD +@HWI-ST575:165:C1P5BACXX:1:1101:20863:3620 +CGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAAGCGG ++ +DHHFHHIIIIIIIIGIICHIEHIIGGHIIIGHGIIIIAHIIHHGIIIHHHD@@B@DDDDDBDDDDDD>CCCDAC9< +@HWI-ST575:165:C1P5BACXX:1:1101:1773:3939 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +DFH@FHHIIBHBGII<GIHHHIIBHICHCHGIIIHGIIIII9DCEFHEHDECCBB<A:ABBBBBCCCBB +@HWI-ST575:165:C1P5BACXX:1:1101:2081:3878 +AGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHHHHFIJIHIGGJIJJIIIJIJIIIIIIJGICHH>DFCDCBD8BDDDACDDB@ +@HWI-ST575:165:C1P5BACXX:1:1101:4430:3860 +CATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHHFGJIJJIGIJJCGIIIHJIIGIIIHIJIFJ@DCGHGIJJ9EEEEEFFFFDDDDDBDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6400:3801 +AGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGAC ++ +FHHHHHJJJJHIJJJJIJIJJJIJJHHIIJJJJHIJIJJIGIGIIIIJJJHAECHFFFDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6719:3898 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHGFHHFIIIJIIJJFHJHIJJJIJJJJGEIJJJJGHIIGGICGHHHHFFFDDDD@DCC +@HWI-ST575:165:C1P5BACXX:1:1101:8167:3757 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DF;CDFFGFFEHFAEGH;?DFBF@EEGEAHEIIFABGEIG;>CGG>EEEBDC3;,;;A +@HWI-ST575:165:C1P5BACXX:1:1101:9714:3894 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAACAAGCCTACGTCTTCGGAT ++ +DHFHHFHIIBEGHIGHHAGGFGGEHIIJJGEHJGGIJJGIGIECEHEEEFF8@@B?C(59998@CDBDD<BAA-82 +@HWI-ST575:165:C1P5BACXX:1:1101:11290:3835 +TGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCA ++ +DHFHHHIJJJJIJJIJJJJJEEGGGGIIJJJJJFFHHHFFFFDEBEEDEDEDD3>CCCACCC +@HWI-ST575:165:C1P5BACXX:1:1101:12038:3902 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHFHIIJJJIIJJFHHGHGGIGIJIGHIIIGIIIIIIIIIIFGIIHJIIHGFDAEEDB +@HWI-ST575:165:C1P5BACXX:1:1101:12700:3779 +GGTCGTGCCTAGTGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +FHHHHHJJJJJJHGIJJIJIIJJIJJJJJJJJJIJIJJJIGJJIJJIIGJJGFFDCEECDDBBDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:12790:3907 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DHD?FF@FGIGBFFEHGBFB<DA@BDHHFHG@FHGCDGHB@GG@GGCCGHBED<BCACA +@HWI-ST575:165:C1P5BACXX:1:1101:13831:3779 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHFHHIJJJJJJJJJJJHEIGGIHJIJIHIIJJJJJJJJJIIIJJHHHHFDCDDBBCC@ +@HWI-ST575:165:C1P5BACXX:1:1101:14731:3871 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHDHHHIJJJJIHIHGIEGDIIJJIIIJJJJJJGJJJIGIIIIIHHHHFFDDDD@AC?? +@HWI-ST575:165:C1P5BACXX:1:1101:14772:3935 +GGTCGTGCCTAGCGAAGTTATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FGFDF?FEHIJEBHJIBGEGIIIFEHEGCHHIJIJJJIFIIIFHIIIGHEDCDDD9A3? +@HWI-ST575:165:C1P5BACXX:1:1101:15067:3763 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHGDFHHIJEGIIEG@FGDG?BGGIGGHH@HIGIIIJJIIIJEEHHFFFDDDD@D7<:59>@BBCCC@A +@HWI-ST575:165:C1P5BACXX:1:1101:15156:3801 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHDHHIJJJJJJIJJJFHJIGIIIJJIJCHIJJIJJJJJJJHHHHFFFFDDDDD?BAC +@HWI-ST575:165:C1P5BACXX:1:1101:16559:3892 +TGCCAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC ++ +DDCDHF:ECFGBHIEEEGFBFCB?DFFDD:??BFHDD=FAFEGB@GF;?>?=?D<>B@>=?&9<<< +@HWI-ST575:165:C1P5BACXX:1:1101:17255:3956 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHFHHIJJJJJJIJJJJHGIIIIIIJJJJIIJJGIJJJJIGJJJJJFIHHFFDD??35 +@HWI-ST575:165:C1P5BACXX:1:1101:17500:3790 +TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +DHDHHDHIIJIIHGI;FIEIGIIJIJJJJIJG@HIJGIIIJFHHGFFCDDDD@ACCD7@B8CCABA +@HWI-ST575:165:C1P5BACXX:1:1101:18440:3926 +CCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACT ++ +FHHHHHJJJJJJIIJJJJJJJJJJDGHJJJJIIJIJHGJJJJIJJJIJJJJJIJIJIJJ +@HWI-ST575:165:C1P5BACXX:1:1101:19111:3987 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHAFHHIIIJJJJIGIJJJGIAGGGHIJIIJGIGJJJIJIJJIIHHHGFDDDDD??CD +@HWI-ST575:165:C1P5BACXX:1:1101:19557:3888 +TTGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHHHFDEGIGIGIJGIIDGGG<GBGHIJJJII@DFBGGHIGIEGGIIGGDHEFFDDAA> +@HWI-ST575:165:C1P5BACXX:1:1101:19920:3801 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHHHHHJIJJJJIJHIJGIIIEHGIJIJJJJJJIIIJJJJIIJJIIHHHFFDDDD@CC5;@??CCCBDADDCDB88 +@HWI-ST575:165:C1P5BACXX:1:1101:1580:4241 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHFHGHIJGJIIHIBGGHDGGEHGIJEHIJGGHHGGIIIIHIDIJIGHHHFCDB??CCDD?<BCC?B<ACBCBD88 +@HWI-ST575:165:C1P5BACXX:1:1101:2986:4163 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHGDHHIIJJJIJJJJJIHJJHEHFGIIIIIIIIFGIIIIGIIIJIH@DA>EECDAB?CBB +@HWI-ST575:165:C1P5BACXX:1:1101:4437:4204 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FGHHHHIIJJJJJIIIJJJIJJJGGJJJIGIJJJBFHIJJIIJJIJJHIIIH<?A?CDC +@HWI-ST575:165:C1P5BACXX:1:1101:5319:4088 +TTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAA ++ +FHHHHHJJIIIGIFHIGIJJIJJJJJGGIIEGIGHIGGIEIIGEFEIIEIGGEGI +@HWI-ST575:165:C1P5BACXX:1:1101:5702:4094 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHIJJJJJJJGHJHIJIIIIIIGIIJHHJJJIIJJIGIJIJJIJHHFFDDDDD=AB@?BDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5824:4111 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHHHJJJJJJJJIIJIJJIJJIJJIIHIJIJJJJJJDHGEIIJJGIHHFFBDD@DCB= +@HWI-ST575:165:C1P5BACXX:1:1101:6984:4013 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +DHHCFFCGFEEHGGEHG<?FEFEGDBFCGE=FGHDGGGEEAFGAEHEDEAA?>C@==>?( +@HWI-ST575:165:C1P5BACXX:1:1101:7890:4076 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTTACGGCAGGAA ++ +FGFHHHJJJJJJEHIGGHIIICHGCFGHHGHGIBGCGHIGIJDGHIHEEEFBAB7= +@HWI-ST575:165:C1P5BACXX:1:1101:8052:4159 +TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DFFFFBEFEHDA?GID?9?B<BFHDF@B>DD?;C<=8BCFHIH@=DAEBBA:5??= +@HWI-ST575:165:C1P5BACXX:1:1101:8080:4229 +TTTCGGCGGATCCAAATCGG ++ +DHFDHFFEHGFGGEHIIIHG +@HWI-ST575:165:C1P5BACXX:1:1101:8526:4228 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJIJJJJJJJJIIIGGIJIJIJJJIIIJIJIJJJJJJIGHIIJHHFFDDBDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8765:4049 +TAATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHHGHJJJJJJJJJJGIIJJIIIIJJJJJJJJJJJIFIGJGHGEHHHFEFDFDCA?1?BDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9822:4042 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHFHHJJJIJJJIJJJJIIJIGIIJJJJIJJJIJIIJJJIJJJIIIIGIJHHFDAC3AB +@HWI-ST575:165:C1P5BACXX:1:1101:10184:4066 +GTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJJJIJJJJJJIIJIIJJIIJJIJJJJIJJIIJJJGECHHFFDDDBBCD9 +@HWI-ST575:165:C1P5BACXX:1:1101:11794:4113 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAGCCTACGT ++ +FHHHHHIJIJJJGHHIIHGGHGIIJJJJJIJJIGIGJJJJIIJJJJDGHFFFDDDBCDD?2<ACDBA +@HWI-ST575:165:C1P5BACXX:1:1101:12356:4148 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHBHHHIIHGJJIJJJBGHHIIJIIJIIGGIJJJHIJJJJIIIIJHFFHDFDDDBDD?B +@HWI-ST575:165:C1P5BACXX:1:1101:14554:4015 +TTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FGHHHHIJJJJGHIJJIJJJIIIJIIIJGIFGHHFBCDBDDBBCDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14736:4035 +TCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGG ++ +DFHHDFIGIIJIIHJHGHJCGGBFGHHEHII?@@FDEEEDH<BBBABAD??B>?BB?@DB +@HWI-ST575:165:C1P5BACXX:1:1101:15267:4200 +ACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCG ++ +FFHHHHIJIJIIIIJIGIJJJFFGHGHFHIJIIIFJJGGIGHHIIHGHFEFD>BBD?BBBDDDDDDDBDDDDA::+ +@HWI-ST575:165:C1P5BACXX:1:1101:17040:4246 +GAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAAC ++ +DDF<BFGGGIIIIII<FHHEGEHCG>FHBGGHIGGIIIIIGIGHHIHIHHIGCHIC=@ +@HWI-ST575:165:C1P5BACXX:1:1101:17659:4106 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHGDHDFIIIIHCHHEG6BHHIIIIGGIIHIIG;;@GIIIHIDCEIHHEDDDDD=?C?<=@?A@ACBDACDDDD@? +@HWI-ST575:165:C1P5BACXX:1:1101:17880:4233 +GGGTCGTGCCTAGCTAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGG ++ +FHHFFHHHAE<?BEEFFC4?GF@@?<FAFDGB1@FHEHEBF>DGBCFEGIGBBBC +@HWI-ST575:165:C1P5BACXX:1:1101:19348:4134 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHDHHHJJIJJJJIIJIIJIIBGBEFFEGEIGGHIJJJJJIJIIIIIJHHF:BABBDC +@HWI-ST575:165:C1P5BACXX:1:1101:1929:4262 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJJJIJJIJIIIJHJIJJIJJJJJHHHHHHGFFFFEDEDBDDDDBBDDDDD?BDCDBD +@HWI-ST575:165:C1P5BACXX:1:1101:3262:4389 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +??FDFF<ECDHEAEEF<1?BFDD@D>GD>FGHHBGCFHEGCH9@D@ACEBEE><?= +@HWI-ST575:165:C1P5BACXX:1:1101:3996:4500 +AAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACC ++ +AHHD:AEGGBFG<FFHEGICDFHGIGG>FGIGFFB:B<BDGF9FDBBFFEG;4;E?BEFCCCBB@ +@HWI-ST575:165:C1P5BACXX:1:1101:4255:4286 +TGAGAGTGGAACGCGGCACACCCCTC ++ +;FFFD<+AE?CFC:@@)?)9BFFFD; +@HWI-ST575:165:C1P5BACXX:1:1101:4583:4479 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHGDHHHIFIIGGGGDGGGGIEHGIIHEGGIGIHHIJJ@DGGJJJFEHFADDBDDC@ +@HWI-ST575:165:C1P5BACXX:1:1101:5886:4450 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DFFFFHIJIIEIJJIEHIIJJFGIGIGHF@GGGIDGGJI9BFEHGGG2=EBD8<8;=?C +@HWI-ST575:165:C1P5BACXX:1:1101:6699:4365 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FFFFFHGGGIIIHIDHGGIGHDEGGIIGBHIGIHGDEBHIEFGIHGGCHEBAA>==?A +@HWI-ST575:165:C1P5BACXX:1:1101:7054:4268 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHFHHGIJJIJJJJJIJGIIGEIGIIJJIJJJJJJJIGGGGIIIIGHHDDCCDBBDCC +@HWI-ST575:165:C1P5BACXX:1:1101:7021:4304 +ACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG ++ +FHHDGHIJJJIJJJIIJGIHIJJJJHHIJJIJGIIJJJJCCEAHCHB?CFC@CEEDEEDDDDDB +@HWI-ST575:165:C1P5BACXX:1:1101:7330:4272 +TTTAAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCT ++ +FFHHHHJIFHIHJIIJHHHIJGHJIIIIGIHJJGDHICG?FHEIIGIGIGIDGGEEECCECCDD +@HWI-ST575:165:C1P5BACXX:1:1101:7985:4412 +TTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGTGAGAGTGGAACGCGG ++ +FHHHHHJJJJJJJIJJJJJJJIJJJJJJJJIGIFIJIJIJJJJJJGIHIIJIJEHIGGH +@HWI-ST575:165:C1P5BACXX:1:1101:9154:4399 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGG ++ +DAA0CDHE?H@HGGI<GA)?FGF@?@C<F8CC;A@GG=C@>@EC +@HWI-ST575:165:C1P5BACXX:1:1101:9397:4390 +TTGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FGGHFHJIJJJIIJJJJJJIIJIJJJJJJJJJJJJIIJIJJJJJBEGGIIIHHFFDD@CC +@HWI-ST575:165:C1P5BACXX:1:1101:9626:4444 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHGHHHJJJJJJJJJIJJIIIGIIIJJJIJJJJJIJJJJJJJJJGHHHHFFDDDDB@>CDDBBBDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9920:4375 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAG ++ +FHHHHHIJJJJJJJIJIGHIIIJIJIJIIJJJJCGGIIIJIIJIJIJJHGF>CDD@C(;@? +@HWI-ST575:165:C1P5BACXX:1:1101:9928:4484 +GCGAAGTCATAAGCTATGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTC ++ +FGGGHHHHGIHIIJJJJJJIJJJJIIGGIGEHJJEIJIIHIGFFFCECDB?ACCDBDD +@HWI-ST575:165:C1P5BACXX:1:1101:10347:4290 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHHHHHJIJJJJJJHIIIJJIGJIIJJIGJJJJJJJIJGJJJIJJJHHHFFACDBBDCBDB@@CDD;A@CBDDB8< +@HWI-ST575:165:C1P5BACXX:1:1101:10607:4474 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJIJJJIJJIJIIIJGIIJIJFIJJJIIJJJJJJJJJJJHFHF>ADBBD(< +@HWI-ST575:165:C1P5BACXX:1:1101:11109:4422 +GCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGA ++ +D?CFHBACEE@EEHBH?9<CEHGGIC1:?F;???8DDH4B?FFDCBBGHFG;FFHIDE@ +@HWI-ST575:165:C1P5BACXX:1:1101:11719:4366 +TGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGAC ++ +FHHHHHJIJJJJJJJJGHJJJJIJHHIJJJIJJJJJJJJJIIJJJHJHIIJIJHIJJCCAGHFE>AECEDDDBD;8 +@HWI-ST575:165:C1P5BACXX:1:1101:11803:4357 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHFIJJJIJIJIJIHHIIGJGIJJJIIIJJIIIJIIJJJIIGGFHHFDD@BBBCDBBB8<C>A@A +@HWI-ST575:165:C1P5BACXX:1:1101:12622:4263 +AGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC ++ +FDFFHFIIIIAFHGEHEIGIIIGII9CFHGEBFEGHHGIGI@FH;)8CBHEED?D@?=B@BB +@HWI-ST575:165:C1P5BACXX:1:1101:13888:4376 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FGFHHHJGIJJJFGFHIHIIIJJGIIIIHIIJJGGIIIIBGGFIHHGHBDDDBD@BD?B +@HWI-ST575:165:C1P5BACXX:1:1101:14985:4327 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGCCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHFHHIJJJIIJIIIJIGIIIHIHJIIFGGJIJJJJJJIIFHGHEFFFDDDDDD?BC?5 +@HWI-ST575:165:C1P5BACXX:1:1101:15294:4416 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTA ++ +DFHCFF@HGGHIICCFHGDHHHEDBE@F@>=B;A=FHGGI +@HWI-ST575:165:C1P5BACXX:1:1101:16020:4286 +GTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJIJJJJJJHIIJJJJJJIJIIJIJJJJJJJIJJIIIHIIHGFFDDBBCDB +@HWI-ST575:165:C1P5BACXX:1:1101:16371:4398 +CCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHGHJIJIGIHHIJJJJJIIIJIJDGHJJIGIFJIJJIIIJJJHHFFFF@BBBDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17919:4354 +ACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC ++ +BFHHFFIIIHIDHGJIIGEHIHE@FHF:CCCGGGIDFFBDGCA;@@AAEEBA:/=<?B +@HWI-ST575:165:C1P5BACXX:1:1101:19347:4445 +GTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTGGGATATGGC ++ +FHHHHHJJJJJJJJJJIHIJJJJIJJIJJJJJJIJJJJJIIJJJIIHHEHFFDFDEEDCDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19620:4333 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHFFHFHGIJJJJJGGIGIGIEHFHIJJJJJJIHIJJJJJFIJIJIHGHFFDDDBBDD9@B?BDCCBDBCDDDB<8 +@HWI-ST575:165:C1P5BACXX:1:1101:20453:4255 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHFHHJIIJJJJJJJJHIDICGDHHIGGEIGIIJJJJJIJJJJJIHHHFF?DD@=CC<B=ADDD?BD +@HWI-ST575:165:C1P5BACXX:1:1101:20959:4324 +TTATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +DFFFBFGIIIIGG3AFDGHEI@H+AFF@FFGGGFDFFDB8BBEFAEEFCEDD>BACCCBB@B=@ +@HWI-ST575:165:C1P5BACXX:1:1101:20762:4415 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +DDADF8CBHEEGEGHAACGGGCFAEAHDGGIEG8BFHGEEHHGGAED;<HEEACB2; +@HWI-ST575:165:C1P5BACXX:1:1101:21156:4355 +ACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGA ++ +:ADFFDCFFEFIIIF9CFEEG1?CCFFGC??F9:0?FF';D=@CBCFDD>8;B +@HWI-ST575:165:C1P5BACXX:1:1101:21005:4492 +GGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FGHHGHIJIJJIJIJJIJDIIJJIIJJIJJJIIH>DGGHGHFEFCBC?BBDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:2208:4721 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGTATCCTTGAAAG ++ +FHFHHHGJIJGJIIIIJJJCICGGHIJGIIHIIGIGHIGIIHCHHIFCHFFDDB;>CCDEDCD3>> +@HWI-ST575:165:C1P5BACXX:1:1101:3594:4541 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHGHIJDGACEAEEGHEBFEGEGHEHHHHEHGHHGFHGGGGHCCC=BDF???15:9? +@HWI-ST575:165:C1P5BACXX:1:1101:4453:4680 +TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +DHFHFGJIJJIJIGIFHGEHGHIIJJJJJJJHGIJJIJJJJIIIHHHGFFDD?=>CDDDB@CDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4574:4514 +TTGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAAT ++ +DFHHBFHIJJHHIJIIGIIGGIJJIJJJJJGHJEAE?>BDDDDD@DDBBBBDDD@CD +@HWI-ST575:165:C1P5BACXX:1:1101:4512:4554 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHGFHJJJIIJJIHIIJIIIIGIJJJJFIJJIJIIJJJJIJIJJHGHFF@BDBBDCBD@BDDDDDB +@HWI-ST575:165:C1P5BACXX:1:1101:6091:4740 +GGGAAAAAAGCCTACGTCTTCGGATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTC ++ +DHDHHH;E@C;CEFHHBGGIEDB=FDFGHIIHGDCHGGGIEHGICHCEEFGH;CDEF?BCBC +@HWI-ST575:165:C1P5BACXX:1:1101:7755:4724 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +DFA@DDHIBDGGGBG@A@?FHBHIGCFGE>GHHB@GHIIIE@;=@A?=CEE'=@@<A +@HWI-ST575:165:C1P5BACXX:1:1101:10497:4685 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHFHHHIJIJJJJJIIJGIJIIGFHHHGHFFFDECEEEEDDDDDDDBDDDDDDDB?9C +@HWI-ST575:165:C1P5BACXX:1:1101:13319:4694 +TGGTCGTGCCTAGCGAAGCCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +DAFCF?<EEGEFE@@EC?)11?<D@AAE*88BB@?BFFCFF@C9CE9@=@<?B8?7;; +@HWI-ST575:165:C1P5BACXX:1:1101:14064:4581 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +FHFHHFHGJJIIHGCGHHGIIIGIGHHIIJJIJJJJJJIJIGIJJJIHHHF@CD??C3?;/9<CCCB +@HWI-ST575:165:C1P5BACXX:1:1101:14735:4724 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHGFDHHIJIJJJJJGIJHIJJIEHIEHJGGIJJEHIJJIFGHGIIJHJJHGEDD?A9= +@HWI-ST575:165:C1P5BACXX:1:1101:15633:4606 +ACACGACGCTCTTCCGATCTGAACACCAGACGG ++ +DHHHHFIIJJDHIEHIIIJJHGJJIEGHCEEHG +@HWI-ST575:165:C1P5BACXX:1:1101:16660:4702 +GGCCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTAC ++ +FHHGGDGEHIIIIIBFHBGGDHIIIIIIIFFFH=BFGGIIIGIEHHFHHDBCBB??:(,351<CAC +@HWI-ST575:165:C1P5BACXX:1:1101:18593:4710 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FFCFHHGGIDHGEF@GG?FGBGGGHIGDEFGGH8BCHGIIDGIJCGIGBHDEDB=;A +@HWI-ST575:165:C1P5BACXX:1:1101:19241:4545 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHCFFHIBHIIIHHHIIHIJJIICGHIGFHIGIGHHHHHHDFFFDEEEDDDBDD8AC9? +@HWI-ST575:165:C1P5BACXX:1:1101:19601:4684 +CCCGGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +DHHHFFGGIJIGGIJHIGIJIIJCHGIJJJJGIJIJFFFFDCEDDDDDDDDBBDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20709:4735 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FGFHHHIJJJIIJJIIIIIGJ@DBGGIJHJJJJJIIJJJJJJJJJIIIGFFFDDBBDC +@HWI-ST575:165:C1P5BACXX:1:1101:21224:4560 +AGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCT ++ +FHCFHHJIIJIHIGIJJJGGIJJGIIJJIJJIGGDFHIJDHIJIIIJIFIIEHH>EEFFDC>/=8:BBA05 +@HWI-ST575:165:C1P5BACXX:1:1101:2354:4948 +GGTCGTTCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FDDFDHIGHGHIJJIGGHJGIIJGIJGHGHJJJGIGIIEGHEHFEHFBDDDDDDBDDDDDBBDDD?@A +@HWI-ST575:165:C1P5BACXX:1:1101:2921:4941 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FGFDFHHIIIGHGGIGEHHIJ@GHIIIJIGDIGIH>FGGGHEEEEEF>EDFDD?B@?C +@HWI-ST575:165:C1P5BACXX:1:1101:4132:4919 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGHGHGIJJJJJEHGJJIIJHIIGCHIJIJJJJJIJIJJIJFEFFFFFEDD@BD@DD<BDB<ACDDDA +@HWI-ST575:165:C1P5BACXX:1:1101:5510:4838 +TGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCT ++ +BHHHDHIIHGIIAGIEC?C@HIIIICHGIII<D>??D:DDDHIIDIIEAGEHAEEE +@HWI-ST575:165:C1P5BACXX:1:1101:6248:4986 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGHFHHIJJJJJJJJGIJJJGIIJJJJJIJJJJJJJJIJEHEHHFFFFEDDDDDD<BCD<>B@@CDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6936:4836 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHJJJJIGFHIJJJJIHGIIJJIIIGGIJJJJJJJIJIBEHIJHEFDCCB?BAC +@HWI-ST575:165:C1P5BACXX:1:1101:7709:4867 +AGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAA ++ +FHFHHHJJGJJJIJJJIJIJIGIII?FGIJJFHIIJIDIJJIGGAHIHIHEE>DADBBDDBBBDBB;<9@@BDD>A +@HWI-ST575:165:C1P5BACXX:1:1101:9439:4978 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHGHJJJJJIJJJJJJJIJJJIJJJIIJJJIJIJJJJIJIJHHHHFFFDDDDD@B?CDDDBDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9572:4907 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHFHHHJJJJIIIIJIIGIJIIJGGJIIJIJIJJIJJIIJFEIGIJJHGFF:BDBDA( +@HWI-ST575:165:C1P5BACXX:1:1101:9900:4892 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FGHFHHJJJIJIJJJJJJIIJGIIHIIIGIIIJJHGIJJJJIIJJJIIIJJHFFDDEACB +@HWI-ST575:165:C1P5BACXX:1:1101:9950:4989 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FGHHHHJJJJJJJJHHJIJIJGIHHJJJIJJJJJJJJJJJIJJIIHGIGHFBCDDDD( +@HWI-ST575:165:C1P5BACXX:1:1101:10290:4901 +GACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC ++ +FHHHFGGFHIGIHIIJGBFEGI?FHGHIGGBHGGIGIGHHIGHGGHHFEFDDDDDDBBD +@HWI-ST575:165:C1P5BACXX:1:1101:10794:4755 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHHFHHIIIJJJJJJHIIGIJIJGIJDIIIIIGJHGIJIJJGGIJJJIJEFFFDDBD( +@HWI-ST575:165:C1P5BACXX:1:1101:11203:4970 +AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGGFHHJJJJJJIJJJJJIJJJJJJJIIJJIIIIFHIJJJFHGIHIIGHIHHFFDDD3=ABB@BDCCBD +@HWI-ST575:165:C1P5BACXX:1:1101:11597:4816 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHFJJJJJJJJJJJIIIJJJJJJJJIJJJJJJJJJJIGIIIIJDGHGFFDDDDDCDDDBDDCCDB +@HWI-ST575:165:C1P5BACXX:1:1101:11814:4971 +CCGGTCGTGCCTATCGAAGTCATAAGCTAGTGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHGHFHJJJJJJIIGIIJJIIIJIJIJJJJJJJEGBFGIJJIEIJIIJIJJHH<>CA?CCD +@HWI-ST575:165:C1P5BACXX:1:1101:12815:4804 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHFHGDHIIJIJGHIHGGHGIGJGIJJJIIIJIIIIIJJJJGJFII;DAHBECDDBC +@HWI-ST575:165:C1P5BACXX:1:1101:15064:4795 +TATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHHHHJJJIGIJJJGIIIJIJHIJIHIJJIJJJJIIIJIJHFFFECEDEDCDDDDBDBCCCD +@HWI-ST575:165:C1P5BACXX:1:1101:15338:4874 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FFCFHDHGHIJJIJBHIFHIIIJIJJEGFFEHIF9CFGHI@GGIGEFHHFDDDDB=C?B +@HWI-ST575:165:C1P5BACXX:1:1101:15739:4984 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +DAAACHDGGGIICG?FD?FGH@@GBCBB2.=CF=@D@CH;==@A==?A<BB?33,555( +@HWI-ST575:165:C1P5BACXX:1:1101:16043:4995 +GTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHIJJJJJJJJJGIIEIIIIJJJGIJJIIJJJJJJJJJJIEHIHHFFDDBDDD? +@HWI-ST575:165:C1P5BACXX:1:1101:17608:4859 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +DFFFFFG@FGIHHH?:DBDDGGGHGIHHCHGEHDEACAHIHIIIFFHEEECCB>89?5< +@HWI-ST575:165:C1P5BACXX:1:1101:17879:4904 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +FHGHHGIJJIJJJJJJJIGGGIIJJJJIJIJJJJIJIJJIGHIIIJHHHHFFDBD?A +@HWI-ST575:165:C1P5BACXX:1:1101:18072:4949 +AGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGT ++ +FHGHHHJGIIJJIJIJJJJJIJIII@GHDDGGGIIIGIJIIIGEHHHF?BAEECDDD=B; +@HWI-ST575:165:C1P5BACXX:1:1101:18453:4916 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHFHHGGIIIIIHEFHFHGHIJIJIIAHIIIHHIIGIIJIJIJIJHHHEF@CB==?(9 +@HWI-ST575:165:C1P5BACXX:1:1101:19254:4780 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGGCTGACGGCAGGAAAAAA ++ +AFADFHGGFGIIEHAAG?GEHGEGBGDGEGGGIF>@CFFHIEEIHHHFFCBCB@?5?B +@HWI-ST575:165:C1P5BACXX:1:1101:19861:4798 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJIJJJJJIJJJIIIGHGIJIIIJJIJHJJJJIJIIJJIIIJHHFFDDBBDD=BBBBC@CDD +@HWI-ST575:165:C1P5BACXX:1:1101:19870:4937 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHIGIIJJHIGGIFHIIEGGEHGHIIJJIIGIIJIJJIGIIIHHHFFDDDD?CD +@HWI-ST575:165:C1P5BACXX:1:1101:19796:4989 +AAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FGHHHHJJJJJJIJJJJJJJJJJJJJJJJJJJJIJJJJHFEFFFFDEEEDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20053:4972 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGHHGHIJJJGIIIGHIHIJIADGHIHHGIJJJJJHIJIIHIJIIIIJHGFDCDBBDD????ACCCBD +@HWI-ST575:165:C1P5BACXX:1:1101:20782:4908 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHFHAHIIIIHICHE0DGGII>?DFDGGHIIEGAGIGIGIGIIBGE;CBDD@BDBB?C +@HWI-ST575:165:C1P5BACXX:1:1101:1189:5021 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTC ++ +AF22<@F@?CFFCF>EG7CDGCCBDAGHGFDFGB> +@HWI-ST575:165:C1P5BACXX:1:1101:1686:5115 +GGTCGTGCCTAGGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGCCAGGAAAAAAGCCTACGTCTTCGGATA ++ +FHHHHHJJJJFHIJIJJJJJJIJJIGHIJJJIIJIJJJJIIIJHGHIG'=EDF?>;AEDD@BCCDDDDDDDDB?<< +@HWI-ST575:165:C1P5BACXX:1:1101:2938:5127 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +?6CDHFIEDHHDHHGHHBHGGGIIGGGGEFHCECFEA>F;=@AEHD>DAEB<83=;? +@HWI-ST575:165:C1P5BACXX:1:1101:3405:5036 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJIJIJJJJJJJJGIHIIIIIJIJJJJIIJJJJGCIIIJIGHFEDD?BDDBB=BBDCDBB +@HWI-ST575:165:C1P5BACXX:1:1101:7045:5134 +GGTCGTGCCTAGCGAAGTGATAAGCTAGGG ++ +D=DD8@@=EC<33<C?):*:C?D>9:*?:? +@HWI-ST575:165:C1P5BACXX:1:1101:7123:5216 +TTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATG ++ +FHHBHHJJJJIJIIJEHFEGIJJIIIGIIHIGIJGIGDGGIJJJJJJJJIJJIJEHFHG +@HWI-ST575:165:C1P5BACXX:1:1101:8358:5145 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +FHGFDHEHIJHEHEFBDCAA@ADDCCDD@BDD@DCDDEDCCCDDDB2<?BDBB;B8? +@HWI-ST575:165:C1P5BACXX:1:1101:8840:5173 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHIJJJJJJIGIJIJJIJJIIJJHIJJJJJJIJHHHHGDFFFEEDDDBDD?BCA +@HWI-ST575:165:C1P5BACXX:1:1101:9731:5160 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAACAAGCCTACGTCTTCGGAT ++ +FHHHFHIJJJIJJIHHGGIIIIJIIJJJIJJIJJIJJJJJIJJIHGGHFDD:@BBDD(>B?ABDDDDDDCBDB-22 +@HWI-ST575:165:C1P5BACXX:1:1101:10830:5146 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHGHHHIJJIJGIIJEHIIIIGGGHGEIEHIJJIGHGIIJGHIIJHHGGFFDB@B@?( +@HWI-ST575:165:C1P5BACXX:1:1101:11032:5068 +TCTTGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGAC ++ +BDHHHHIIIIIGIAHHHGIIIHIII@HHCHIIIIGIDEHIIIGGIDFGDEIHAHFFFFBCCCB8? +@HWI-ST575:165:C1P5BACXX:1:1101:11565:5085 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHFHHHIEIGIIGGGGIHIGHGGEEGHGIIIJJHGGGJEHGHJIIHECHFDD?B=A?CD +@HWI-ST575:165:C1P5BACXX:1:1101:12209:5017 +AAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAAT ++ +DBAFFFF2AE<?AE<FEH?ECGF?0BBB;BD;@@:@F;:4=@;@BBB8=@=@@B5<:>AB +@HWI-ST575:165:C1P5BACXX:1:1101:12070:5238 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATAT ++ +FHHHHHJJJJJJJJJJJIJJIIJIJJIJJJJJJJIJJJJJIJJJJJJHHFDDD9;?AB:9ACDDBABCBCDB?854 +@HWI-ST575:165:C1P5BACXX:1:1101:12614:5234 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGAAAAAA ++ +FHHHHHGIJJJIJIJIJJIJIIIJIIJIIJBHIJHIIJJIIJJJJHGFHEFDDB@CDDBD +@HWI-ST575:165:C1P5BACXX:1:1101:12934:5066 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJJJIIJJJJHIJGJJIJGIJJJJIJJIJIIIJJJJIJHHFFD@?ACDBDDABDACB? +@HWI-ST575:165:C1P5BACXX:1:1101:12889:5153 +TAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHHHHJJJJJJJIIJJJIIIJJJJJJJJJJJJJBHIJJJJGIHFEHFFFFFDDDDDDDDB +@HWI-ST575:165:C1P5BACXX:1:1101:13006:5008 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTA ++ +:88<@C<EBD@BCCFIF:0??B>D48DDF>;C@F=@@@@E +@HWI-ST575:165:C1P5BACXX:1:1101:13701:5054 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHHHJJJJJJJJJJJJJJJIJIJJJJIJJJIJIJJJJIJJJIIHGHGDDDDDBDDDBD +@HWI-ST575:165:C1P5BACXX:1:1101:13818:5190 +TTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTGGCGGATCCAAATCG ++ +DHHHHHJJJJIJJJCBG@HIHIIGIJGIJJJJGBFGFIJJJJJIFDDDDDDDDDDDBDDBDDD@CCCB +@HWI-ST575:165:C1P5BACXX:1:1101:14721:5211 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGG ++ +DFCDF1@AEFEF9CC@FBDBFFFF*9?F<=BDF8BF>FEFEEDCEEE9=B>'9> +@HWI-ST575:165:C1P5BACXX:1:1101:15180:5074 +GTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJJJJIJIIJJJJJJJJIJIJJIJJJJJJJJJJJJJIIGIJHHFFDDACB +@HWI-ST575:165:C1P5BACXX:1:1101:15733:5028 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHJJJJJJJJJIJJGIJJJIIJIJIIJJJJJIIJJJIIGIJIJIIJJHFFDBE9A +@HWI-ST575:165:C1P5BACXX:1:1101:16783:5148 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHDHHFHJFJIJIIIGHBFHGJJIJJJJIGIIJJJJJJJJGIJJJIHHHFFDDD?AC(5 +@HWI-ST575:165:C1P5BACXX:1:1101:17093:5065 +GCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJIIBHGHIJJIJJJIGIGIIJJJJIIJJJJJJJJIEFFBEE9;8>ABDCDD? +@HWI-ST575:165:C1P5BACXX:1:1101:18177:5052 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHGHJIJJIIIJHJJHJJJIJJIJJHIIJJJFHIIJJIIJJJJIJJHHFFDD?BCAAB +@HWI-ST575:165:C1P5BACXX:1:1101:18454:5064 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FAFHHHIIIJIJJIGJI?GFGIIGIGIHIGIJHIIJIIGHGIGIIIFHHFFDDD=BC +@HWI-ST575:165:C1P5BACXX:1:1101:19514:5155 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHFDHHIGIJGIIHDDHIIGIEFHIIGGGGIEHFHIIIJJHGIJJIIGIIHGFD?@EC +@HWI-ST575:165:C1P5BACXX:1:1101:21070:5172 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FGDFHHJIHGIIGH@FGDHGGEGHGHGGHGIGIGGGGHIGGIIIIDAGHE<99;??CC +@HWI-ST575:165:C1P5BACXX:1:1101:1152:5446 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DDACF@EGHGGGHBGDHDDGAFD<D9?D9DBGDBFGCFHG@A@@GD>E<AB6A>??>C +@HWI-ST575:165:C1P5BACXX:1:1101:2332:5476 +ACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHHHHHIIJJJEHIJHJJIJIGIHIJJHHJIJHIJJIIJJDHDDCDBDDDDBDDBDDDDDD>ACCBB +@HWI-ST575:165:C1P5BACXX:1:1101:3549:5422 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJJJJJIIIIJGIJIIJJGHGHHFFFFFEEEDCEDDD?CBDDBDDBACDDDDDBDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:3934:5255 +TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHGHGFIJJIJJIJJIJJJJIJIJIJJJJJJJIGIJIGJJJIIIIIJJHHFF7>CCEDDD@DDDBB +@HWI-ST575:165:C1P5BACXX:1:1101:3779:5257 +GTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHGHJJJJJJJJJJIJIJJJJIJJJJJJJJJJIIIHJJJJIIGFHFFDDECCBB@BBDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:3981:5396 +GTGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +DFDFHFEHBGECHBGGG@??B@?F>FBFFCHF7F/BFCHEGG4@FHH;EHD=49=;2 +@HWI-ST575:165:C1P5BACXX:1:1101:4352:5435 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHIJJJJJIJJJIJJJJJHHJJJJJIJIIIIIJJJJJJIIJIJJHHFEDDDDDDDDDBDCCCBD +@HWI-ST575:165:C1P5BACXX:1:1101:5757:5381 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +D?FFFF@GHIIGIGIIEIDFGIIIGIEGFB@DFIGHGIGIIGEGIIIHIG64'=>;,>3 +@HWI-ST575:165:C1P5BACXX:1:1101:7084:5290 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHGFHHIJJJJIJIJJJJJJIHIJGIJJHGIJJJJJJJJJIJIIHHHGFFFDDDDBDD +@HWI-ST575:165:C1P5BACXX:1:1101:7550:5435 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHHFHGIIIJIJJIJEHGHIIGJJIJJJC@GIJIJHIIJJJJHIFHHDFFDDDDD@AC +@HWI-ST575:165:C1P5BACXX:1:1101:8518:5454 +ATCCGCTTCGGCGGATCCAAATCGG ++ +D?FDHHJJJGBGIHIIIJJJJIIHG +@HWI-ST575:165:C1P5BACXX:1:1101:8740:5499 +GGGTCGTCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJJJJJJJHIIHIJJJJGGIIJJJJJIJJJJJJJJHEEIFHFFDDBBDD2 +@HWI-ST575:165:C1P5BACXX:1:1101:8810:5337 +TGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAAC ++ +FHHHFHIJJJGJIJJIHHIJJJJICFGHIJIJJJIJJIJIIJJJJJICFHG7CFHIJBH +@HWI-ST575:165:C1P5BACXX:1:1101:10317:5279 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTTTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTTGGAT ++ +FHHHHHJJJJJJJJFFHIIJIJJIJJJJJJJJJJJJJJJJIJJJJHHHHFFBDDDDD3?BB<ACDDDDBDDDD?44 +@HWI-ST575:165:C1P5BACXX:1:1101:11346:5362 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHHHJJJJJJJJIJJJJIIIJGJJJJJJJJJJJJJJJIJJJIGGHIHFFDD=;?;?B +@HWI-ST575:165:C1P5BACXX:1:1101:12293:5318 +TTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGC ++ +DHDFHFIIGIGGGHFFFGHIJEHGGGHGGGGFG<BGFEEGGEGHFHCGE@=CE====E +@HWI-ST575:165:C1P5BACXX:1:1101:12385:5426 +GGTCCTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJIJJJJIJJJIIIIHIJJJJJJJJIJJJJJIIJJJJJIJIHHFFC?CCCBD@2?CACDA +@HWI-ST575:165:C1P5BACXX:1:1101:12559:5359 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGA ++ +AHCDF?C?CGGG@GGIGB4??FDGIIIE>=@F@=.=C??EC +@HWI-ST575:165:C1P5BACXX:1:1101:13619:5383 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FGHHHHJIJJIIIIGEHGIJJJJIJIJJIJIJJJIJJIJJIJHHHFFFE@BDDDBDD +@HWI-ST575:165:C1P5BACXX:1:1101:14813:5367 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FGFHHHJJJIIJJIHIIFHIIJGIJJJGHGIJJHIHIIJIJGHIIIJJIIHFFDACE9 +@HWI-ST575:165:C1P5BACXX:1:1101:16958:5440 +AAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACC ++ +FHHHGHIIJIJJJHIJJJJIIJJIIJJJGIGHJJJIFHIJIJJJJJGHIJJIHHHFFFFDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17064:5269 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJJJHJJIIJIJJJJJJJIIJJJIJIJJJJIJJJJJJIIIHHFDDEC3=@???CDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18711:5374 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHHHJIJJJJJJIIJHIIIJJIJJJJJJJJJIJJJJJIIJJJJIIJHHFFDDBAA=;= +@HWI-ST575:165:C1P5BACXX:1:1101:19795:5388 +AAAAGTGCCACAGGGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGG ++ +B<FFHFGE?GEFG+AFHCF<DGF?FGIIC<GEEGGF*?<FEG<FGGHIIB@B +@HWI-ST575:165:C1P5BACXX:1:1101:1536:5742 +TTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCA ++ +DHHHDFGHEHEEHIFBFGGHFEEGG=6=@DEHHFHHFFDCDCCEA??=;>CCCCD>CC>A@:>:CC +@HWI-ST575:165:C1P5BACXX:1:1101:3186:5524 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHGHHHIJIJJJJIJIJJJIIIJGIJJJFIIJJIJGIJIGIEGIHGEEABDDBBBBDDDDDBBCDDBB +@HWI-ST575:165:C1P5BACXX:1:1101:3099:5544 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGHHHHJJJJGGIJJIJGHGGIIGIGIIGIIJJJIJJJJIIIIGHGHHHDDD/;BAC?B?;ABCCDDA +@HWI-ST575:165:C1P5BACXX:1:1101:4347:5721 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +DHFHAFGDHIIJIIGGGFHEGIJFIHGII?CEGHIIIIIJIIJCHHGGBEF'9@BBDCA +@HWI-ST575:165:C1P5BACXX:1:1101:5268:5661 +AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +DDD?D<<EBHBEFF:E1?1??DECBFFE9)0??@BBBFGBACBCFH;@CAA='3=;?5;5 +@HWI-ST575:165:C1P5BACXX:1:1101:6568:5658 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHIJJJJJIJIGJIJIIJJIJIJJJJIJJJJIJJIJEIDGIHIJHHFFDDDACDD:;?ACDCDA +@HWI-ST575:165:C1P5BACXX:1:1101:7344:5519 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHGHHHJIJIJJJJJJJJIIIJJIJIJJJIJJIIJJJJJIIJIIIHGGFFDDDDBDDDDDBBDDDDDB +@HWI-ST575:165:C1P5BACXX:1:1101:8216:5553 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHHHHHHJJIJIJJJJJJJIGIIJIIJJGJIJJJIIJJJJJIIJHHHFFFDDDDD?BC +@HWI-ST575:165:C1P5BACXX:1:1101:8183:5554 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +:C1@D@CF?EFEFFI8CD?9BGDA@FFD?;F;FFFC>>AFCFA9;@EFFE<@9'95? +@HWI-ST575:165:C1P5BACXX:1:1101:8832:5696 +TTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAA ++ +FHHHHHJJJJHIIEHJHJIJIIIJEGHIJJJJJJJJJIJIJIIIIJHJHFHDFCDBDDDDBDDDBBD9>9<>?:@A +@HWI-ST575:165:C1P5BACXX:1:1101:9211:5626 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGACAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJJJJJJJJJJIHJIGIJJJJJIJJJJIJJJJJJIJIIJJHHFDDCDDC(= +@HWI-ST575:165:C1P5BACXX:1:1101:9028:5675 +TAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTGGATATGGC ++ +FHHHHHJJJJJJJJJJJJJJJJJJIJJJJJJJJIJJJJJJJJJJIBHEEEHFFFFEDEECDEDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9789:5670 +GGTCGTGCCTAGCGAAGTGATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJIIIJJIJJFFEHIIGIJGIIJJJJJIIJIJJIJGIGIHHHFF@BB=AD9A +@HWI-ST575:165:C1P5BACXX:1:1101:10507:5654 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DFDDFHIGGGHI@FCGFBGGGICG>FDBBDCAFD>GHI;CGA2@E@=?<B<>?B5(>( +@HWI-ST575:165:C1P5BACXX:1:1101:12148:5538 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHFHHGGJGIJJJJIIIGHGIIIIIJJIJJJJJADHIIIEIIHEFHFFEDDDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:12149:5686 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHGFHHGIJJIIJGIHGIDGIIJJIIIJJJJJFECGHIFFHHHFFFFEEDDDDDD?BCC +@HWI-ST575:165:C1P5BACXX:1:1101:12546:5747 +TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGACAAAA ++ +DHDDHFGEFHHGGIJCEF?@DCGIEGBDBHE?BFDHHIIGAGGFAFBEEFFD?(6;;? +@HWI-ST575:165:C1P5BACXX:1:1101:13615:5616 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJJJJJJIJJJJIIIJIJJJIHGIJJIHIIJJGIJFHIHGFEEFDDDBDD?B +@HWI-ST575:165:C1P5BACXX:1:1101:13537:5735 +TTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAA ++ +FHHHHHJJJIJJJJJJJJGJJJJIGIIJGHIJJJJIIJIJHHGHHFFFFECECEEEAACD +@HWI-ST575:165:C1P5BACXX:1:1101:14584:5542 +TCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGA ++ +FHHHHHJJJJJJIJJJGJFFHIJJIEIJIIHIJJJJGIHIIIIJJJJGHGIJJJJHIEGHGEHEHE +@HWI-ST575:165:C1P5BACXX:1:1101:15724:5746 +TGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCG ++ +FGHHHHJJGHJJJJHHIIJIIIJJHIIIIHIIJJIJJIJHFFDDDDDDDDDDDDDDD:A@CCB +@HWI-ST575:165:C1P5BACXX:1:1101:17227:5625 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +DDCFFHEFGGGIEHGB?0??DBDB3BD@BDDBFBGIIICH@AFGIGE>AEDA<?;=?(5 +@HWI-ST575:165:C1P5BACXX:1:1101:17881:5507 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHIJJJJIJJJIIIHIJIIIIJJIJJJGIJIJIJJIJFIGIJGGIJHHFDDAA;=A +@HWI-ST575:165:C1P5BACXX:1:1101:20112:5554 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +DHFDHHIGHIIIGHEHIHIGHCFGGIGDFHIEHIIHIIIIGIGIIGHGHHEBAA=?C??@:2<CCCB +@HWI-ST575:165:C1P5BACXX:1:1101:20322:5548 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTA ++ +@D<CDCE8CB<C?C::?09DDADDDDDDDDBB@8=CEII +@HWI-ST575:165:C1P5BACXX:1:1101:21233:5679 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DFC@FFD1?FHICF:FEFHBEBBFFDG=@DHAECCEEHDCDD@CCCCC?BB;=B88?< +@HWI-ST575:165:C1P5BACXX:1:1101:1959:5920 +CCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCC ++ +EHHHHHIIJJCHIJIJHG<FHIJJFG?FHIEHHIAHHGIIJGGHGIIGIJJGHIJJFHFFFFF?BDDD>B=B955? +@HWI-ST575:165:C1P5BACXX:1:1101:1924:5978 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHDFFFHFIIJJEHIJGBGHHGHFHHIGEIDHGFHGGGEHIHGJJHDHEFADDD??CCB +@HWI-ST575:165:C1P5BACXX:1:1101:4153:5778 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHGHHHJJJJJJJJJIIIJIGIJIIJJJIJJJJJJIJJJJJJIFGHHHFFDDDDDDD5<@B(8CDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4024:5788 +TTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJBGHIDFHIEIGGIJJJ@HGJJIJGIJIJGFHIGIJJGIJIHFDBBCDDBDDDDCBB +@HWI-ST575:165:C1P5BACXX:1:1101:4463:5945 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGG ++ +AD@FHDFEHEHGGIIIE??BBG@DGC>CAFCHICHGI>CH;?=B?66;9' +@HWI-ST575:165:C1P5BACXX:1:1101:6188:5778 +TGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +DHHHHHJJIIJJGIJJJJJJJGIJIJJJHIIJIJIGJJJJJJJJEHHFDDEC@BDDABDA?@D +@HWI-ST575:165:C1P5BACXX:1:1101:6867:5946 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJJJJJJJJHIGHIJIJJJJIIJJJJJJJJJJIJJJIJJJJJHFFDDEEDD +@HWI-ST575:165:C1P5BACXX:1:1101:7535:5814 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHDIJJJJJJJJJJJJJJJIGIJJJJJJJJIJJJIJFJGIJHHHHFFDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7794:5903 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FGHDFHEHIIJJJJJIJIBHGIIIGIBHGHIIIIEHIIJJHHGEFFFFFCDDDDD?ACD2 +@HWI-ST575:165:C1P5BACXX:1:1101:9106:5832 +AGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAA ++ +FGFHHHIIDIHHIJGJJJJJJJIJJHIIGEHGIDIHIGIGGHHI@EHIHDHFFFBBBDDDBDBBDDDDDB@<<<:> +@HWI-ST575:165:C1P5BACXX:1:1101:9392:5986 +AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FFGFHHGIGIBGIEIE?FCFEGGFFHGEGFGGGEGFGFGHHEHHFIEDGGEAB/>=A(5 +@HWI-ST575:165:C1P5BACXX:1:1101:9549:5793 +TAAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACC ++ +DDDDDD2CEEEEEFAC:F?CEEEEEEIEE4CDFDEDDBDDDDEIDDDDEDDDD@<ADDD +@HWI-ST575:165:C1P5BACXX:1:1101:10541:5867 +CGGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHHHFHJIJJGJJJJJJIJJJJGIJJIJJJJJJJJGIJJJJIJJDHHHFFFDDDDDB9A +@HWI-ST575:165:C1P5BACXX:1:1101:11397:5818 +GCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +DBF<?<A:<EAEEBC??<?;<A;BBFGIBD6B?F)B@;6.=@@4 +@HWI-ST575:165:C1P5BACXX:1:1101:13482:5805 +TCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCCGG ++ +FHHHHHJFIJJJJJIBHHJGIGIIJJJJJJJIJ@DHFHIIJIIJJGFC@BDDDDDDBBDBBBDDA:@ACCDB +@HWI-ST575:165:C1P5BACXX:1:1101:13731:5797 +CGGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHGGFHJJJJIJIJIJGIJIIIIGGHGGIIIIJCH@CHIHHGFHHDDFDEDDD>@DBB?CB +@HWI-ST575:165:C1P5BACXX:1:1101:13983:5888 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHFHHHIFJJIJHHGHIGIJIIGIIJJJJIJGJEHIJJIJIJIJJJHHGFFBDDBDC5 +@HWI-ST575:165:C1P5BACXX:1:1101:13915:5939 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHFHHFHJJJJIIJIIJHIIIJJJJIJJIIIJJJJJIJJIIIFGIFEEEDDBDDBBD(5 +@HWI-ST575:165:C1P5BACXX:1:1101:14076:5802 +ACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCG ++ +FHHHHHJIJJIJIIJJJJJJJEGGIJJIJIIFHIJJJJJIJIHGHJHHHFFDDDDDDDDDDDDDDDDBDDDCC>@2 +@HWI-ST575:165:C1P5BACXX:1:1101:14165:5883 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHDFFDHEGIGIIGA@DFHGEIIGIEGIIDGJIEGIGGGEHGGHGIGHHHFDBDB?C?? +@HWI-ST575:165:C1P5BACXX:1:1101:14716:5991 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGTCAGGAAACA ++ +FHFHGHIJJJJJJJJIHDHGHIJIJJJJJJJJJGHIJJJJJJJIIHHHGFFFDEEDD(> +@HWI-ST575:165:C1P5BACXX:1:1101:15153:5829 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTCTAGAGGCTGACGGCAGGAAA ++ +DHHDHFIIGHGHI>?FDH6BDFGG@FBGIBFHBGGHGGCGIGEHHCEBCAC>9=?(9> +@HWI-ST575:165:C1P5BACXX:1:1101:15618:5813 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGG ++ +DDFDAFGIJEIIDIGHBFGGGIIHGIIIAFDHIHIEDHCGEHFGCHHHHDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:16278:5962 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGGCTTT ++ +DF8DFFDGE@EFFG:?D?DDAFIDFFEAECFIF':AEF +@HWI-ST575:165:C1P5BACXX:1:1101:17404:5768 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHGDHHGIIGEHGGJBHJIGIIJIIGIGIHJJGHAHIJJIGIEGGHHGHFFACDD7<:< +@HWI-ST575:165:C1P5BACXX:1:1101:18508:5770 +CGTGCCTAGCGAAGACATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHGHHHJIJJJIIJIJJJIIIJJIJJJJIHGIIJJJIJIJJJJAEHHHDDD?=ACBDDDDDDDBB +@HWI-ST575:165:C1P5BACXX:1:1101:18654:5893 +AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHHJIIJIJJJIIJIIJIJIIJJIJIIJIJJJJJJIJGGIIJJIHIJHEFD?>35?@@9<CDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20005:5823 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FDHDHHI=FHIIIHEFHAGGHCFGGHIHHIIIIFIIIIIIII@@FGHGHGFBCC?=A?; +@HWI-ST575:165:C1P5BACXX:1:1101:20461:5768 +GGGGTCGTGCCTAGCGAAGTCATAAGTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FGFGDAHFGIIJJJGIGDGFDGGFEHIEFFEHEGHHIIGIJ<EGHHHEHFFDDDD7A? +@HWI-ST575:165:C1P5BACXX:1:1101:1693:6048 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +DFF<<CF8AB@EFDF8CD:D9B<DFFDFCF?FBFBFFFIFF=FFCEEIEEED69@?BBBB: +@HWI-ST575:165:C1P5BACXX:1:1101:3291:6004 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHHHHHJJJJJIJJIIJDHHIGIGHJJJIJIJJIJJJJJJIIIHHHHFFFCDDDBDD +@HWI-ST575:165:C1P5BACXX:1:1101:3937:6198 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAACAAAAGCCTAC ++ +DHFHFHGI7EHGEBFFHGBF9@BEDGEAFHIE;AFHCGHECFGCGGI>ABBBBBB;A(<5<95<ACC? +@HWI-ST575:165:C1P5BACXX:1:1101:4273:6038 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHFHHHGJJJJJIJJIJCFDHIIIIJIGHIIJIGH@FHIIIIIDIGFHGDD:ADBDD<A +@HWI-ST575:165:C1P5BACXX:1:1101:4445:6115 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHFDHDHIIJII>DHIDHIBFGCDHFCHEHGGIIHJGIJGGJJFFHHHDE>AB@BBA3 +@HWI-ST575:165:C1P5BACXX:1:1101:4344:6153 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAACAAAGCCTACGTCTTCGGA ++ +FHHF)@GHIJJJIGIIJJJIBGIIIIIJJIJJGIIIIJJJIIJGEHHHHDDDCDDBD(,(5??ADDDDDDCDDDB< +@HWI-ST575:165:C1P5BACXX:1:1101:5212:6077 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DF@FFFHJIIJJIIJII?FDHHIGIJGGGGIGGHHIIIJJGGIGHFHDDB<>BB??C< +@HWI-ST575:165:C1P5BACXX:1:1101:5854:6193 +AGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHHFFHEHIJJJJJJIJFHIJFIBHH<FHIIJJJGGIIGIJJII<EHFDDDDDBDDD=BBBB5;>AAC@CC25 +@HWI-ST575:165:C1P5BACXX:1:1101:6093:6246 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAACAAAGCCTAC ++ +FHHFHHIIEIGEIIIEBHFGBFBHIIJGIGIGHJHGHGGGHEGGGIJ9=ADDBA??B295<>?BAAD +@HWI-ST575:165:C1P5BACXX:1:1101:8105:6158 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FGFHFFHGIJJJIIGIIDFIGIEHHIGIGBFDHIGIIGGGHEGGGEEHHFD?@B7=( +@HWI-ST575:165:C1P5BACXX:1:1101:8253:6223 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGCAA ++ +DF@DFCFFBFFIBCDF:0??DFAGFI<8?FE;FFFE@F@CFE@EE>>CEDC/>:',5 +@HWI-ST575:165:C1P5BACXX:1:1101:8552:6031 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACTGCAGGA ++ +FGA@DFFEGHEH@FGDGGBBDGIGIGGJEGGGIGID=AGIJHHJJG?;CHEEFFF7 +@HWI-ST575:165:C1P5BACXX:1:1101:8575:6101 +TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +BDDDFHFHEGGGIIEHGGHHGCH@HGGGGBG?GG?@6CFH<CE;CHHCEHEDDFED>?B?CDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9569:6015 +ATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATC ++ +FHHHHHJJJJJIJJJJJJJIJIJJIIJJJJJJJJIIJJFIIIGH@GIIGIJGGIGIJHFFFFEEDDDD'38;@90) +@HWI-ST575:165:C1P5BACXX:1:1101:10905:6024 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DHFADFEGDFEEEDGI@:?CFGDGD@FDFB?BF@?DFEFGB<<BFH@=C@BBBDCBC3= +@HWI-ST575:165:C1P5BACXX:1:1101:11477:6010 +TCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAAT ++ +DFHGHHIIJJJJHJJCHHJGGHGHJJIIIJGGIBDGHHIIDFHGIFFCDBDBDDD@?=BBDBDDCCCC +@HWI-ST575:165:C1P5BACXX:1:1101:12389:6154 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHG?FHFHEIJIGHFHGIFHIIGJCGHGJJGGFGHIIJJHI@DGEDHEHHECABB;<C3< +@HWI-ST575:165:C1P5BACXX:1:1101:12820:6019 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGG ++ +AFH@DAFHBHGIJJIIIEGGGGEHBGIGCAGGIGD@CGH@EHHEDFFECADDD@D +@HWI-ST575:165:C1P5BACXX:1:1101:13503:6047 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHIJJJJJJJJIJJGIIIJJIJJJJJJJJIJGIJJIIIJJJIJIJJHFFDDDBDCB +@HWI-ST575:165:C1P5BACXX:1:1101:14071:6041 +AGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTC ++ +FFFHDFGDHIGDHCHEGE9ED?FFBG?DFGBFHIBGIGGHGGHGGFEED@G=FDCCDG +@HWI-ST575:165:C1P5BACXX:1:1101:14685:6102 +TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHHHHJJJIJJIJJJJIJJJGJGIJIJJJJJJJJJJCHIJJJIBHHFHFFFFDDDD7??CDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14930:6229 +GGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGT ++ +FHHHHHIFHJIJIJJJJJJJJJIIIIHIDHIJJGIJJFHGHFFFFC=A@CCDDDBABA +@HWI-ST575:165:C1P5BACXX:1:1101:15280:6053 +ATCCTCTTCGGCGGATCCAAGACGG ++ +FHHGHHJJJJJJJJIJIIJJIGIIJ +@HWI-ST575:165:C1P5BACXX:1:1101:15344:6191 +ATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGAC ++ +FHHHHHJGGIJHHIJJJIJHIIJJJJJIIIIJJJJIJIIJJJHHGI@GFHIIIJIJIHE';C@CDDDD@BB +@HWI-ST575:165:C1P5BACXX:1:1101:16410:6086 +GATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAAC ++ +EHHGHHJJJJJJHHIIIIJIIJJJGGFIGIIJIJJJIIIIJIIIGJJJJJIJJJIFGEHAHHGIHHH +@HWI-ST575:165:C1P5BACXX:1:1101:16374:6145 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHFHHGHGIDEGEG@GIFHIGIGAGHCCDHI;ADEEHJIGIIJJJHHHFFBCBB;ACD5; +@HWI-ST575:165:C1P5BACXX:1:1101:16749:6158 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHDHHHIJJJJIHIIIIHHIHIIIJJJJJJJJJJJJJJIJIJIIJJHIJJHFFD=BDCABB?@DDDBB +@HWI-ST575:165:C1P5BACXX:1:1101:17553:6192 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTAC ++ +FHHHHFHIJIHHHIGGHGGIHJJJIIIIIFIIGGIGIJJIIJIGGIGAEEEFDD;;??BDBA9ACC +@HWI-ST575:165:C1P5BACXX:1:1101:17938:6081 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHFHHHIJJJIJJIHIIFHIIIJJJJIJJJJJJJJJJJJJEIIJJHHHHFFDDD@BD<?B@BBDDDDDBDDDDB<8 +@HWI-ST575:165:C1P5BACXX:1:1101:19315:6150 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +DDHAFCGIGDHH9E?FEDD<DFF<9DHGCFHIIHIIIIGGGIIBHIGGHHDDCB@=CCBBB<<CC?BABCBCB@8+ +@HWI-ST575:165:C1P5BACXX:1:1101:19532:6018 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHADHFHEHHHHDFFHGFEIDHIG@FGHIJIIGHGJIIGHEHIIHHEEFFCDDD19>3? +@HWI-ST575:165:C1P5BACXX:1:1101:21261:6248 +TATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGA ++ +FHHHGHIJJJJJBFHJIJIJJGIGFFGEIJJJJEHJJIIIIEG@FHIHIGIAGGDGGGGE +@HWI-ST575:165:C1P5BACXX:1:1101:1089:6262 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FH@FFDGIIIIIGHHFH?FGEGGIFG>GGHEHFBGHEFGFHIG=;AAHEFFBBB?ACCAB +@HWI-ST575:165:C1P5BACXX:1:1101:1439:6319 +TATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +2C?<F?GF:ABFA;C?CCHG9F*??8:CAGBF:@FF;C3=C;-=A==79;.7?CCB;?/@5>< +@HWI-ST575:165:C1P5BACXX:1:1101:1829:6451 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGATGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJIGHEFFHGEFGIIIIIJJIJJIJIJIJIIIJIJJJJJBHIJJGFF?@CCB@D??CC?=A +@HWI-ST575:165:C1P5BACXX:1:1101:2484:6444 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +DFFAFDFEFGGIIIGGBBFGIJEGIIDD8FGGE8==CH@@D=@E@AAEHEBB<;5;? +@HWI-ST575:165:C1P5BACXX:1:1101:3350:6364 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGC ++ +FHDFFHEDEEFGHIIGB0?A9BAFFAGGHE@AFHGAGE>AAEHH +@HWI-ST575:165:C1P5BACXX:1:1101:3699:6336 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGA ++ +DDDFHHEG;FHI>F?F@?CFGEEH>D@<B?DFHGGBHFFGG@@@G>(@B/=>>8( +@HWI-ST575:165:C1P5BACXX:1:1101:3874:6280 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGATGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGA ++ +FHHHHHJJJJJJJJJJJJJJIIJJJIJJJHIJGIIHJJJJJJJIJJHHHHFFDDDDD(99@BDDDDDDDDDDDDD< +@HWI-ST575:165:C1P5BACXX:1:1101:4404:6415 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHFHHHJJJJJJJJGIJGIJIJJIJJGGGIJJJIJJJIJJIJJJJJ;EHHFCDD=BD39 +@HWI-ST575:165:C1P5BACXX:1:1101:5416:6451 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAACA ++ +FHHGHFGHIJIJIJIIE@FFIIIIJIJGFIIJJIJJIJJJIGJJCHCEHBHFFDDBE(> +@HWI-ST575:165:C1P5BACXX:1:1101:7066:6455 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHHHHHJJIJJJJJIJJIJJIJIIJJJJJJJJJIJJJJJJIJJIIJIHHHFBDD@AC +@HWI-ST575:165:C1P5BACXX:1:1101:7488:6251 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHHHHHJJJJJJJJJJJHJIIJJIJJJJJJJJJIJJJJJJJIHHHFFFFDDBDDD?C??B>BDCDDDDBCDCDD?8 +@HWI-ST575:165:C1P5BACXX:1:1101:7486:6329 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHGHHHIIJJJIJIIJEHGHIIJJGGJJJJIJJJGIIJJJJGGIJIIGJJIHBEDA@C=B +@HWI-ST575:165:C1P5BACXX:1:1101:7643:6450 +ACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHHHFHHGGGHGIGJGBHGHIGHIIJEGHHEFFDB@D8A@CCCCBB +@HWI-ST575:165:C1P5BACXX:1:1101:8454:6487 +TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHFHHIIIJCHHGGHGGGHHFHFHEHJJ@FHIJIJICGHGHIGIJHEEFFDFFEDCDD@>CDD +@HWI-ST575:165:C1P5BACXX:1:1101:9137:6329 +AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHHJJJJJJJIIJJIJJGIJIIIJJIFJJJIJIJJJJJJJJJJIJJHHFCD??;? +@HWI-ST575:165:C1P5BACXX:1:1101:9587:6277 +GCGGTCGTGCCTAGCGAAGTAATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHFHJHJGIJJJIJIIJFEHIGICHIIGGIFIJGIJIJJGHHDFFFFFDDD?D@?5>AB +@HWI-ST575:165:C1P5BACXX:1:1101:9536:6348 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHDHDHIIGIJJIJHIIEHIHGGHDGHEGHGGHHGIG@C=GIAHHGEHFHEDCB=55,9 +@HWI-ST575:165:C1P5BACXX:1:1101:10054:6289 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJIIIIJJIJJHIGHIGIJJJHIGIJGHGGIJI@HGGJJGGHFDDDDD;>,9 +@HWI-ST575:165:C1P5BACXX:1:1101:10173:6294 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHHHJJJJJJJJJJIGGIHJJIJJGJJIJJJJJIJIJJIIFIJJIIJJHHFD;6;(;; +@HWI-ST575:165:C1P5BACXX:1:1101:10764:6333 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHFFDDEGBFFHHIGGGHGHEHIEFGHIGBGIGEHHIIGHCCHHFFFDBCBBBB155(95 +@HWI-ST575:165:C1P5BACXX:1:1101:11047:6477 +GCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +BDFFFBGIGFFGFGHIEIIIG@HG@:GEDBHGIGG;5@;=@E;AEE@DFDCCC6A@CB +@HWI-ST575:165:C1P5BACXX:1:1101:11411:6372 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHFFFHJJIIGIIJIFHEFCHHGHGIGGIFFHHFFFFFFEEDEEDDDDDBBD@BD?ACC? +@HWI-ST575:165:C1P5BACXX:1:1101:11665:6448 +GGTCGTGACTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHFHHFIEIJHIHHHIIIHIHIJIIJHIJJIIIIIJJJIIIJIIGIHHHHFDDD@ACDDDDABC@<@? +@HWI-ST575:165:C1P5BACXX:1:1101:12774:6457 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FGH@FFDEHIGCHGHGHIFHHIGJEHIJJGIJGH@@DGGGIGHIBCEHCBBD>>@=(:?5 +@HWI-ST575:165:C1P5BACXX:1:1101:13106:6369 +AAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTC ++ +FHHHHHJJJJJJIJGJJJJJIIJJJJJJJHIHIJJJJJJJJJJJJJJIJIJIHHHHHFFDE +@HWI-ST575:165:C1P5BACXX:1:1101:16704:6321 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHFHHIJJJIIJJJJIJGIJIJJJIJIJIJJJJJIJJJJJJJJJIJIIIHHFFDDEDDD +@HWI-ST575:165:C1P5BACXX:1:1101:16660:6380 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHFHHHJJJJJIHIGIIGGCHGIJIIIIJJJJJJIJJJJJEIJJJJIGIJHHFD5@CCB +@HWI-ST575:165:C1P5BACXX:1:1101:17964:6393 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +FHHFFHIBHGGEIHHFHIGGHHC>FBGIGIJI7F@DFHGIEGCHGEHGFFFDDDD5? +@HWI-ST575:165:C1P5BACXX:1:1101:18173:6282 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHFFHGHIJJJJJJHGJEHJIJJJIJJJJJJJIHIJJJJJIJJJJHGFFFDDDD=AACBDB1<ACCBD +@HWI-ST575:165:C1P5BACXX:1:1101:18209:6492 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGTTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHDHHHGJJIJJHIFGHBHIIIJJJJIIJJIJJJI<FHGIGIIJJJIHHHFDCD5?CDBDDABCDCBBDDDD@D<< +@HWI-ST575:165:C1P5BACXX:1:1101:18546:6430 +TTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAA ++ +FHHHGHJJJJJJJJJJJJIJIJIIEGHIJIJIGJJJJJIJGHHHHFFBBCCEDEDECCCC +@HWI-ST575:165:C1P5BACXX:1:1101:19023:6315 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGAGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHGFHHFHJJIJJJJJIJIJIJIGIIJJIGGIIIHIJJJJIIIEGIHGHIJHBDFDDEEE +@HWI-ST575:165:C1P5BACXX:1:1101:19100:6466 +CGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FGHDHHIIJIJIIIJJHIGGEGI<HIIBD@FCBFGGIJIFHCE;DHEAHHFBBDDBDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20025:6400 +TTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAA ++ +DHHHDHIJJJHGIJJIHHCH9CGBF2BGHIIJJJIHIJG?EEHFGGFBBB@AC>AEDC@C +@HWI-ST575:165:C1P5BACXX:1:1101:20043:6415 +ACGACGATCTTCCGATCTCGGACACAGACGG ++ +DDDAFF6DEFIIFIECG>FFI>FIFFEBE'5 +@HWI-ST575:165:C1P5BACXX:1:1101:1982:6500 +ACGACTCTCTTCCGATCTGGACACCGG ++ +FHHG?FHIEGIH@GEHIIIJJIJGCHH +@HWI-ST575:165:C1P5BACXX:1:1101:1934:6651 +TGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +DHFHHHIJJJJIGIIHIGIIIJJIJIJGIJIIJJJIJJJGIIFIIIFIJIHFFDCCDCDBBBBDCCBD +@HWI-ST575:165:C1P5BACXX:1:1101:3031:6587 +ATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHGHHJGHJJJJIJIJJJJIJJJJJJJJJJJGIGIIIIJIFHHHHFFFEEDCD:B?BDCCD +@HWI-ST575:165:C1P5BACXX:1:1101:3659:6632 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHFCFFEHJIIIJIJGGIHHGIIJIGIJIAGH?HGIIJJEIGGEHHHFFFDDBBB?B:5(0 +@HWI-ST575:165:C1P5BACXX:1:1101:4271:6639 +GGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGA ++ +FGGHHHJIIJJJEIIIJJJBHGIIICHCFGIJE?GDH9FHHJJI)=8@FAFFGIEHGIH? +@HWI-ST575:165:C1P5BACXX:1:1101:5521:6516 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAACAAGCCTACG ++ +FHFHHFIIJJIGEHHHBDGFIIIIJIGIIIJIFGIIJJJIGCHIIIEHH>BCDB?BC3?(,98C>CB +@HWI-ST575:165:C1P5BACXX:1:1101:6888:6616 +GTTAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGA ++ +DADFB<+C?GBH?GFGF8CDF<?FD@?6.7@AB;<E?D<?9'98259 +@HWI-ST575:165:C1P5BACXX:1:1101:6970:6733 +TTATCCTTGAAAGTGCCACAGTGACGAAGTCTCATAGAAATGGTGAGAGTGGAACGCGG ++ +FHHHFDHHJJJIGBFHHJJJJJIIHIGDHHIIGHEIJCFHIIIFGIH<FHGEE@HGEGE +@HWI-ST575:165:C1P5BACXX:1:1101:9786:6627 +ACACTCTTTCCCTACGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCT ++ +FHGHHHIJJHJJJJJJJIJJJJIIIIIIIGJJJJJGIEIIIJJIFGIHGGGHDBDBCFDD +@HWI-ST575:165:C1P5BACXX:1:1101:10466:6616 +GAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTC ++ +FHHHHHJJGJIJJJIJJJJJJJIIJJJJJJJJGIJJJJJJGIJJIJJJJHHHCHFDE1ACDA +@HWI-ST575:165:C1P5BACXX:1:1101:12094:6626 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHFHHFIJJJIJJJJJJIJIIJJIJJJJIJJJJIJJJJJICGHHHHFFFDDDDD<BCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13236:6588 +TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +DHHHHHJJJJJJJJJIJIIIJJJJIJIJJJIIIJJJJJJJIJJJIHHGFDDDDBADDDD9<CCCBB +@HWI-ST575:165:C1P5BACXX:1:1101:13123:6721 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHHHJJJIJJJJIJJEGEHIJJJIJJJIIJJJJIJIIJIJJJJJIIHGFBDD?ADDBB +@HWI-ST575:165:C1P5BACXX:1:1101:13878:6710 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +FHHHHHIJJJJJIHGHIHJIIJJIJJJJJIJJJJJIGJIIGIGIIHHHFFDDDB;?CDD;5<ADDCB +@HWI-ST575:165:C1P5BACXX:1:1101:14181:6588 +TCTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTT ++ +FHHHHHJIJJJJJJJJIIHIGIJJJJIGIIIJJIJJIHIJHHHHEHEFFDDCE@CEED +@HWI-ST575:165:C1P5BACXX:1:1101:14627:6506 +TTGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAACA ++ +DHDDDFHFHEHGIJGHJJJGGGGBHHGGGHIICHGCHHIIHIIJIIIGGFHHFDDDBDD3> +@HWI-ST575:165:C1P5BACXX:1:1101:15075:6709 +GGTCGTGCCTAGCGGAGTCATAAGCTAGGGCAGTCTTTAGAGG ++ +DFFFFDGBGB?ECF)0?0:9BGG<9B>3DF8BF.8@@F>@C>@ +@HWI-ST575:165:C1P5BACXX:1:1101:16741:6696 +GATCCGTTCGCATTGACACTGCTTATACGTGTAGACGG ++ +FHHGHHJIIJJGJJIIJJGIIJJJJJJJJJJJJJIJJJ +@HWI-ST575:165:C1P5BACXX:1:1101:16765:6674 +AGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHHHHHIIIJJJJJHIHIJJJJJJIJJJJJJHHHHFFDDDDDCDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19358:6651 +ACACGACGCTCTTCCGATCTATGATACGCGG ++ +DFFFFAA)@FFEC@F:?BD>?DF@FEECFAF +@HWI-ST575:165:C1P5BACXX:1:1101:19561:6627 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCA ++ +DDDDDCEDEIEC3C?D@:?DECDEDDDDBDDDA=BBDEEI>@>CDDCD8=<? +@HWI-ST575:165:C1P5BACXX:1:1101:20819:6735 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +FHGHHHIJJJJIIJJHGIIIJHIIJIIIGIJIJJJIIJJJJIIGHIJHHIIHFFDDEEDD?=ABCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:1564:6811 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +FDCFFHGGGHIIGH?FFBGGHGHHGIDFEDHIIIGCGHAG=DCHFA>?BDBB@859?C?@BBB@(9; +@HWI-ST575:165:C1P5BACXX:1:1101:1927:6913 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHJIEGII<FGIJHI@DHHIIJJGHII@FFEHGIIGHGCFIGGHHGF<BB??CC +@HWI-ST575:165:C1P5BACXX:1:1101:2572:6857 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHFHHHJHIJIIIJJJJGIIGHIIIJJIJGJIIIIJIIIIIJIHHHHFFFDDDDDBDCA@@?BDDDDBBDDDAB?7 +@HWI-ST575:165:C1P5BACXX:1:1101:3215:6869 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTAGAGGCTGACGGCAGGAAAAAA ++ +FHFHHHJJIIJJHHIJJHDFDGGIFHJHDIIGJEIIJJIJIBGHGGHGFFDBBBCCDD +@HWI-ST575:165:C1P5BACXX:1:1101:3121:6916 +TGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTC ++ +FGFHHFJJHGGJJJIJIGGIJJJIIGDDHIJIIGGIGHH@DHHGHIGIDHHEHG@CCG +@HWI-ST575:165:C1P5BACXX:1:1101:5462:6854 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHFHHHJJJJIIIIEHIHIIIJFIIIGIIJJJJJJJIIIIIIIJJJGHHEDBCD1;C( +@HWI-ST575:165:C1P5BACXX:1:1101:6911:6752 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHFHHHJJJJJJHIHIJGIGGHIIIJJJJIJJJHIJJJJFIJJJHHGHFFDDDD=?CCD +@HWI-ST575:165:C1P5BACXX:1:1101:8402:6992 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +DFF0@DEG7EEBGD:::?899?BF>@DAF>=BC58@@EFCFEEAEFF>@=@?'3;59>@?@ +@HWI-ST575:165:C1P5BACXX:1:1101:10199:6779 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHHHIJJJIJHGIJIHGGIGIIIJJJJJJJJIJIJJIJIJJJHHHFFFDDDD<5:,<= +@HWI-ST575:165:C1P5BACXX:1:1101:11749:6891 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FFFFFHJIJJIJIJHIJIJJIIJIGIIJJJJIJIIIJJJJIIGIJIJJHHFDDC?ACDB?@?ACDDBA +@HWI-ST575:165:C1P5BACXX:1:1101:12769:6834 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCCGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJJJHIIJJJIJJJIJJJJJJJJJJJJJJIIIJJJIIJHFDDDDDBDDDDD@BDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13711:6990 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHDHHGIGIIFIIIJJIIJIGJJEIJJJIJIGJIJIJJJJDGIIJJIIEHFDCDB?CD? +@HWI-ST575:165:C1P5BACXX:1:1101:14111:6893 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHIJJJJJJIIJJHIIIJJJJJIIJJJJJJJJJJJJIJIJJJHHHFFDDD?BDDD6=A@CCDDB +@HWI-ST575:165:C1P5BACXX:1:1101:14440:6994 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHFHHHIIJGIIIJHJJHIJIIJIJJJJJIJJGFHIJJJIGHIJHHFHFFDCDDBDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15694:6869 +ATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAAC ++ +DFFD??>FHICHGHHIIIIEDHHGHHIIIIHIGFHHBDECCCBBBBB?85@BCC(:< +@HWI-ST575:165:C1P5BACXX:1:1101:16093:6801 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAACAGCCTACGT ++ +FHHHHHJIIJJIJJJIJJJIJIGIJJJJJJIJJIIIJJIJIGHJJJJGIIHHFDDDB>A(=<AACDDBB +@HWI-ST575:165:C1P5BACXX:1:1101:16736:6834 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGTCAGGAAAAAAGCCTACGT ++ +FHHHHHJJIJJIJJJHIJIJJIIGIIIJJJJJJJHIJJJJJIIIJJJJIJHGCDEFFDCADD?BCDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:16688:6982 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +DH@:DC@;EFBGAFE@FBFFEFHCDHEGE=BHBFGHIIGE>@FGGIEEEDFBAB;ACC< +@HWI-ST575:165:C1P5BACXX:1:1101:17036:6768 +GGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG ++ +FHHHGFGIIIIGIIHIGIJJIIJHHFGFFFFFDCDDDCCCDCBBDDDDDDDDCC3>AC@ACACBDCCCCB +@HWI-ST575:165:C1P5BACXX:1:1101:17542:6830 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHFHHHIJJJJJJJIJJIJJIJFHIJJJJJIIIHJJJJIJIJIHHHHFEDD?BB<BDCDDD@@CDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:18093:6840 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHCFHDEHG@HIGIGGHFGGIGGDAGIIIJIIIIJJIJIJEGHDGIGJGDEDBD(,;?A +@HWI-ST575:165:C1P5BACXX:1:1101:18333:6967 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAG ++ +?F<ACDCDCBGE@EGA<6BDHH9FFGH</8D7FGI@FEGGCGBDHA2?EDBCB +@HWI-ST575:165:C1P5BACXX:1:1101:19230:6988 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJIJIHIIJHIJIIJIJJJJJJJJJJJJJJJIIJIJJHDHHFDDDDBDDDB +@HWI-ST575:165:C1P5BACXX:1:1101:20486:6800 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHFIJJJIJJJIIJHIIIJJJJJJJIJJJJJJJJJJJFGJJIIGIGHFFDDBDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20297:6938 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTCTAGAGGCTGACGGCAGGAAAA ++ +DHFHHFFHIGHIEGA)?D<FHIIIIGHFFG@GGBCGIIGIIIIIIIEHHEECBBBAC? +@HWI-ST575:165:C1P5BACXX:1:1101:20449:6990 +GGTCGTGCCTAGCGAAGCCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHHFHFHIJJIJIJGHIIJJJJJIDHIIJJJJJIJJJJJJGJJJIJIIIHHFDDCBDD?BDBDDDCDDDDDCDB?8 +@HWI-ST575:165:C1P5BACXX:1:1101:1103:7035 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTC ++ +FHDHHFIJJJIHHHGGIDFGGGIIFHIIIIHIHGIIFIIIIJJIFIFHHFFDBD=ACD??B@AC@<92? +@HWI-ST575:165:C1P5BACXX:1:1101:1500:7064 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTC ++ +FHFFHHIIIJIF:C@AGB?FGEHGDGGIFIBFFAFHGGGIAHCCHECADDDCDB89:?A;B?(:@?>?B +@HWI-ST575:165:C1P5BACXX:1:1101:1577:7179 +TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHGHGHIIIJJJIAHI@GIIH@HCHCEGGG?FGDBHH;BCHGIGAAEECEE9@DEDD?BDACCD +@HWI-ST575:165:C1P5BACXX:1:1101:2297:7148 +TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATGGC ++ +AFHHHHGIIIIIIIIIDGDHIGHGIIIIIIIIIIBFGBHIIIGHEHHGFFFDCAEABBD?BD
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/reads2.fastq Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,2684 @@ +@HWI-ST575:165:C1P5BACXX:1:1101:1666:1132 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +HIJ0??FFCDB?FGDBAEHHBDDDB?@;8?==@8B<B@B+:>87<BB8CCCC?CDD:<@:4ACC>,4::4:(+4@< +@HWI-ST575:165:C1P5BACXX:1:1101:2239:1101 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACT ++ +JJJIGHIGBHHIJJIIGIJH6BDDBBDDBDDDDBBBDBD?CCBDDDDBCCDDDCCDCDCA:@A34(++4+4:: +@HWI-ST575:165:C1P5BACXX:1:1101:2455:1083 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTT ++ +GBGIIGGD@DGHGGGGIAEABBFBDB>B?@=:@BDB<8B9@C8-5<<BD@A><CDCAB>C>>AC4+++++4+44 +@HWI-ST575:165:C1P5BACXX:1:1101:3207:1238 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHIJJHIIJJJFII@EHEEDFABDDDDDDDDDDDBBB>CDBDBDBDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:3405:1152 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +GG8:F6B*?3?B;@;;'54==46<>89'5=6,5-0&&0?(:+5;77-<CDC: +@HWI-ST575:165:C1P5BACXX:1:1101:3512:1179 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIJJJJIJJJJJJIJGIIFBDFDDDDDDDD=@DDB@@BCCDDDDDDDDDDDDDCCC +@HWI-ST575:165:C1P5BACXX:1:1101:4473:1182 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTC ++ +DGGHJBDHGIF@B;BF4;BEE?E@?B@BBDD=B@575<B?CCDBB@BD?ACCC +@HWI-ST575:165:C1P5BACXX:1:1101:5162:1092 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIGHIJIJJJJJIJJIEHHGFDCDDDDDDDDBDDDD<BDDDDDDDDDDEDDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6810:1177 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCGT ++ +HIFHIJJJJJIIGJJJJJHGFFDDDDDDDDDDDDDB7BDDDDDDDB@CDDDDDDDDDDDDDEE:444++:++4>?? +@HWI-ST575:165:C1P5BACXX:1:1101:8268:1157 +CCGATTTGGATCCGCCGAGGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJIIIJJGJIHHFDDDBBDDDDDDDDDBDDDDDDDDDDDBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8740:1185 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIJIIJJJJJJJJIJJJHHFFDDDDDDD=BDDDDDDCDDDDDBDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12414:1099 +CCGATTTGGATCCGCCGGAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJJJJJIJJJJJIJJIJHHFDDDDDDDBDDBDBDBBBDDDCBBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14053:1112 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJIJIIIIJJJHHFFDDDDDDDDDDDDBBDDDDCDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14219:1152 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJGIJJJJJJJIJJJJIIHHFFDDBDDDDDDDDDBDDDDDDDDDDDDDEDDDDDDDDDDDEEE:(++4(444:AB +@HWI-ST575:165:C1P5BACXX:1:1101:15012:1211 +CCGTTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIGIJIJJIJJIJJIJIIGHGFFDDDDBDDDDBD@@BDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15774:1231 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +IJJHIJJIJJJJJIJJGIIHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDDCAACED4(+,++4 +@HWI-ST575:165:C1P5BACXX:1:1101:17047:1127 +CCGTCTTGGATCCGCCGAAGCGGA ++ +D@D0?)8*0*/0?86;@';'-=9A +@HWI-ST575:165:C1P5BACXX:1:1101:18461:1188 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACC ++ +BC@?FEGGHDDGEGAC@BHHEFEBBBDBDDB'5<<<5:??CDD;BDD@?DDACDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19911:1138 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJBHIJJJJJGIGIDHHGH>DFCD>BDDBDDDDDD7@BDCCBBDDDDCCDDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:1311:1500 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJIGIJJJJGIIJJJJJIIHHFFDDBDDDDDDBDDBBBD>ACB@BDDDCDDDDDCCDDDDDEEE:4:::4>4::>@ +@HWI-ST575:165:C1P5BACXX:1:1101:2110:1396 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +EHHF@GGG>GHFHIIGIEIBBBBC@?>;8=@BBBB@B8?3@@<<BDDBDEDDCCCDC +@HWI-ST575:165:C1P5BACXX:1:1101:2970:1430 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJJJGJJJIJJJJJIIIGGEFFFDDDDDDDDDBDDBDDDACDDDDDDDCDDDDDDDDDCC@CDE:+++,+444+:< +@HWI-ST575:165:C1P5BACXX:1:1101:4079:1444 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GIIIEGAGGABFGIJJIGBBHDECBD@BBDBBD85979@A@CBBB;BB<CCCDDDC> +@HWI-ST575:165:C1P5BACXX:1:1101:5495:1254 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIJFIJGIIJJIIIIHIJHHFFCDDDDDDDDDDDDDDDDDCDDDDDDCEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:5684:1314 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIJJJIJGHEH*BGGIFHGGIGHHFFFDDDB;BBDBDBDCCCBDDDD?CCDDDCDDC +@HWI-ST575:165:C1P5BACXX:1:1101:6002:1450 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +FIIDHHGIFIIJEIICHGIEDFFDD@D@@DD'77B@BDBCDD8DDDDBDDCDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6163:1455 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJIJJJJJJJJIJHHFFDDDDDDDDDDDDDDBDDCDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6370:1342 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +?;@:@?;DDD?8/8B;A4A:<D<8>39353;;50:<B<?>C()7<D;2?C3@ +@HWI-ST575:165:C1P5BACXX:1:1101:7500:1303 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGIJJJJJJJJJJJJJJHHFFDDDDDDDDDDDDDBBDDDDDBDDDDDEDDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8186:1308 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +FGIBHEHIGIIIJJJIIJJHHFFCBBDDBDBBDBDBB<BDDDDDDDDDDEDDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10049:1341 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GHG??;?GD39?BAC@FEHEBDBABBBB5==275<@BB@AC@9BDDDBDE@C?@CCD +@HWI-ST575:165:C1P5BACXX:1:1101:10737:1349 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIIBDGIJJGHGIJIIIGHEEFFDBBD@?@BBBDDBDDDCDDDDDDBBDEDDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11993:1308 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +JJJIIJIJJIJIIGJJIIIGHFFDDDDDDBDBDDD@:BBDDDDDDDDDDEDDDCCD +@HWI-ST575:165:C1P5BACXX:1:1101:12260:1420 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGGTTCCACTCTCAC ++ +JIJHIJIFJIIJJJJJIIIHGFFCDDDDBDDDDDDD5<BCDCDDBB?CDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13041:1365 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJIGIJGFHGGHIIJJJGEHFFDDDDBDDDBDDDDDDDCCDBD@B@?CECCCDDD@ +@HWI-ST575:165:C1P5BACXX:1:1101:13915:1290 +CCGGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJJHIJIJGIJJIIJIJHHFFDDDDDDDDDDDDDDBBDDDDDDDDDDDEDDDDDDDDDCDEDD4+,+4+4 +@HWI-ST575:165:C1P5BACXX:1:1101:13944:1432 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJHJJJJJIJJIGJJJJJHHFFDDDDDBDDDDDDBBDDDDDDDDDDDDEDDDDDDDDCCDDEE4+,+4,: +@HWI-ST575:165:C1P5BACXX:1:1101:14362:1371 +CGTCTGTGGTGAAGATCGGAAGAGCGTCGT ++ +?@?FH@DGGIGGEGCEGIGIIEGADEEHBB +@HWI-ST575:165:C1P5BACXX:1:1101:14865:1283 +CCGATTTGGATCCGCAGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +?CA:?66DD<B<;C=@FAFE@HBABA>A=;8283<<BBBACDDDDDBBCECC?CDDA +@HWI-ST575:165:C1P5BACXX:1:1101:15341:1459 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIHGJJJJIJJIIJGEHIGGGBEFDCDDDDDDDDDB<BCDDDDDBBDDDDCCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15856:1385 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIHIJJGFIIIJEGIGIIGFFFDDDDDDDDDDDDDDBDDDDBBDDDDCDDDDDCCC +@HWI-ST575:165:C1P5BACXX:1:1101:16168:1446 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +IJIHIIJJJJJJJIIJGGIHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDBD@ADDE4+++,+4 +@HWI-ST575:165:C1P5BACXX:1:1101:16274:1448 +CGATTTGGATCCGCCGAAGGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +=DGBH?DFCGB?BFGI8AHEFFDBBCBC@BD?BB75<9>395BB7<?CDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17532:1255 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +C=FEFHBFEBHGIIIIJGHEEFEDBBDB@BB/:<B:@BBCDCBDDDD?DECDC:@CC +@HWI-ST575:165:C1P5BACXX:1:1101:19852:1368 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJIGHJEHGFHGIIJGIJIGBFFDD;@DB?BBBDDBBDDDDDDDDDDBCCCDDCDDDDCCCDDE@>::4+>44::8 +@HWI-ST575:165:C1P5BACXX:1:1101:20338:1414 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GIG?@DFHFG>F9F@FHGGHFFFC@>>BBBBBDBBB07@CDCB7B@D?<CC>ACDCD +@HWI-ST575:165:C1P5BACXX:1:1101:1782:1682 +CCGATTTGGATCCGCCCAGCGGATCGGTCGAGG ++ +GGFH:8?<B@DBDD@@@;F>AB=<9A8=8?BBD +@HWI-ST575:165:C1P5BACXX:1:1101:2733:1718 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTC ++ +HGGHGIGGGGHHCHIIIBHBEFFDDB8?<@BBB8BDDB@>@A@BDBBBDDCD?CC +@HWI-ST575:165:C1P5BACXX:1:1101:2796:1586 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIJJIEHIJJJIJJIIJHHFFCDDBBDDDDDCDDDBD>CA8;BDBDDEDDDDDDDDCC@CDE:,+4,(444+:8 +@HWI-ST575:165:C1P5BACXX:1:1101:3075:1523 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIBHIGHIJJJJJIJAGAHFFDDDDDDDDDDDBBDDDCCDDBBBDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4251:1515 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHIJJJIJJJJJJJIJJJHHFDDDDDDDDDDDDBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4481:1718 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJIJJJJJJIJJIIJHHFFDBDDDDDD?BDD@7@DD:ADDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4978:1615 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIJJJJJJJJIJJJJJJJHHFFDDDDDDDDDDDDDDDDDDDBDDDDBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6935:1687 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGAH?DBHGIGEHIGIEHEFEBB;@B?BBB:55798?AC:@BDB<@C@CCCCCCC +@HWI-ST575:165:C1P5BACXX:1:1101:7393:1637 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCCCGTTCCACT ++ +IHJJJIAHGIIGE@FGBEGHGFFDDDDDBDDDDBB:79@CCDCBBDDD?CDD +@HWI-ST575:165:C1P5BACXX:1:1101:7744:1585 +CCGACTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCC ++ +B8@@G61D@?*?.81;A/A=6B><?;82,,8777<:@589A:5@DD@?C +@HWI-ST575:165:C1P5BACXX:1:1101:7646:1624 +CCGTTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJIIJIJJJIJJJEHHGFFDDDD@BDDDDDDBDDDDDCDDDDDBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8080:1642 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGA ++ +JJJGIJJJJJJJJJJJIIJFHFFDDDDDDDDBDDD@<@BDDDDDDDDDCDDDDDDDDDD>:CDD4,+44 +@HWI-ST575:165:C1P5BACXX:1:1101:8452:1710 +CCGTCTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJJIJJGGIGFGFDDDDDDDDBDDDD87@CDDDDDDBBDEDDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:8575:1513 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +JIIGHJJJJIJJJIGJGGGHHFFDDDDDDDDDDBDB@BDDDCD@DDDBCDCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8610:1649 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIIJJIJIIJIIFGIEHJJJIJHFEFDCDDBDDDBBBDCDDD<BDDDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9515:1701 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HHEAE?FGG@DHIHGIAAB:B<?A?B>B5;@B595B<:8?ACB77;B?CDC>ACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:9782:1735 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIJJJGJJJIGIJEIJHHFFDDBBDDDD?BDBBDDDCDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10892:1546 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTACTCTCAC ++ +HIIBBFHIIEHIDGIJIGEEEFDDDDDDDDDBDDDB@@BDDDDDDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10875:1631 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIIGIJJJIJJJIJIIJIGHFFDDDDDDDDBDDDB:<BCDDDDDDDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11536:1555 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJHJJJJIIJJJIJJIJJHHFFDDDDDDDDDDDDDDDDDDDDBDDDDDEDDDDDDDDDCCDDE:44444: +@HWI-ST575:165:C1P5BACXX:1:1101:11540:1624 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJIJJJJJJJJJIJJJJJJJHHFDDDDDDDDDDDDDDDDDCDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12292:1617 +CGATTTGGATCCGCTGGAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIIJJIJIJJIII:FHJJJJGJHFFFDDDDDDDDDBBCDCDDDDDBCEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13423:1569 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJIIJJJJJJJJJJIHHFFDDBDDDDDDDDDDDDDDDDDDDDDBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13576:1612 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +JJJGIJJJJJJJJIJJIJGHHFFDDDDDDDDDDDD@BBDDDDDDDDDDDEDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14491:1526 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJIHIIJJJIJJIIJJIJJHFFFDDDDDDDDDDDBB@@DDDDDDDDDDDEDDDDDDDDD@ACCD4(++++4 +@HWI-ST575:165:C1P5BACXX:1:1101:15109:1589 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGG ++ +IJJBBGIIIIEGIIIJHIJHGFFDDDDBDDDDDDDDDBDDDDDB +@HWI-ST575:165:C1P5BACXX:1:1101:16270:1554 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIJHABDAHEGACEEHGEHEA<BCBB?B?BD@D@@@59@ACD@DDBBD<A@>?ACCD +@HWI-ST575:165:C1P5BACXX:1:1101:17059:1656 +CCGATTTGGATCCGCCGAAGCGGATCGGAGAGGGGTTTACCGAGTTCCACTCTAAC ++ +JJJGIIJJJJIJJJJJJIIHHFFCDDDD@D@DDDDDBBCCCDDDB@@@CDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17823:1711 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJAHIIJIJJJJIIJJIIHHFFDDBDDDDD=BDDB<BDDDCDDDDDDCDDDDDDDDDD@CCDE:44:4+: +@HWI-ST575:165:C1P5BACXX:1:1101:18750:1719 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +BBF@@F?HGCHAGBBHGGBHEFFDDBDDD=B@B8<559?9:@B@D<BD?DDDDCCCD +@HWI-ST575:165:C1P5BACXX:1:1101:19397:1535 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJGIJJJIIJJJIJJIGGGFFFDDBDDDDDDDDD@7@BDDCDDDDDDDEDDDDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:19270:1553 +CCGCTTTGGATCCGCAGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +DHHAD@FGJICHGGI8FEHEHJGFF>AC?BBDDDDB@B@CDCDDDDD?DEDDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20160:1653 +CCGAGAGTCCAGATCGGAAGAGC ++ +JJJJJGJFHIJJIDGGHGIGHGH +@HWI-ST575:165:C1P5BACXX:1:1101:20348:1677 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IGIFHIJJI<DEGGHIIIGHHFF>BBDDBDDBDDDDDDDDDDD;BDDBDEACCCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:1152:1999 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJFGJIJJJJJJJJJIJJHGFD<?;BBBDDBBDDBDDD>ACB@BDBDCDDDDDDD:?CC@@CD4++444>:44>8 +@HWI-ST575:165:C1P5BACXX:1:1101:1575:1782 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTC ++ +?DD:FG7D<FD<9=CGF@;BE>B9>988,8;89B8778?(:><@BBDD<CDCD +@HWI-ST575:165:C1P5BACXX:1:1101:2146:1765 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACT ++ +JIJGHJJJJFGIJEHIDGIHEEFDDDDDBDDBBDDDDBD9ACDBDDDDADDDDDDDDDDCCDDE4(,+,,:4: +@HWI-ST575:165:C1P5BACXX:1:1101:2817:1860 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +BB):8??F4?BB?=B;FAE45?43;;335833007<B583@@7;BD32?DAC9::@:8::+4:C4+,+,+444(:2 +@HWI-ST575:165:C1P5BACXX:1:1101:4121:1770 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIIJIJEHIIJJJJJJHJIEHBDCD@D?BDDBBDDDDBBCDCDDBDBDDDDDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4015:1808 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HGBFDGCHIIIJJFGIGIEHHFFBBDDD@DD:5BB@75@C@C83;BBDCCCCCCDCC +@HWI-ST575:165:C1P5BACXX:1:1101:4671:1789 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +GGF>GGE@>GBGGEGHFEGEFDD6>>?6?BD=05)579BC@@BD;;@DDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5190:1986 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCACAC ++ +JJJIJGGDFCHIJCGIGGAAA?DDDBBDDDDDBDDD5:BCDCDBDDBDCDDDD3>AD +@HWI-ST575:165:C1P5BACXX:1:1101:7320:1866 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJGIJJIIJJJJJJIIGJJBHFEDDDDDDBDDDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7508:1798 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIJJGJIIJJJJJIEGHHFFDDDDDDDDDDDDBDBDDDDDDDDDBCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9647:1841 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGCGTTTACCGCGTTCCACTCTCAC ++ +JJIGHIJJJJIJJIJJIIJGGFFBBBDBDDDBDDD@DDDBCCDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11078:1760 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJJJIJJIJJHHFFDDDDDDDDDDDDB@BDDDDDDDDDDDECDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12091:1910 +CCGATTTGGATCCGCCGAAGCGGATCGATCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIIJJIIJJIIJGIJHHIEHDDDDDDDDDDDDDDDBBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13182:1885 +CCGATTTGGATCAGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHJJJIJIJJJJIJJJJJJHHFDDDDDDDDDDDBBBDDDDDDDDDDDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:13475:1836 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIJJIJJJJJIJJJJJHHFFDDDDDDDDDDDDDDDDDDDDDDDDDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14275:1813 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJIJJJJJJJIJJJIJIJGHFFDDDDDDDDDDDDBDDDDDDDDDDDDDEDDDDDDDDDCCDED4+++4+: +@HWI-ST575:165:C1P5BACXX:1:1101:14914:1754 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HIIGHJJJFJJDIIJIIJJGGFFDDBBDBBDBDDDBDDDDDDDDDDDDDECDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15826:1814 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACAGA ++ +GHIDGGCEHHGIIIIGHHGHEBD>BBDBBDDBBBBBB9@?CDDD +@HWI-ST575:165:C1P5BACXX:1:1101:16316:1995 +CCGATTTGAATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +FGG:FDHHGIAFCHIGI@G=:BB<>9@B?BBBDDDBBB@CCCDDDBBDDEAC?CDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17677:1880 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJG0@FGGGEEG@HIGEIGAA9@88/8@?;;65<BB5<BC>A:3;@<D9>@C?@CDD +@HWI-ST575:165:C1P5BACXX:1:1101:18640:1881 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIJGIGJCHIIIJIIJIIIHHFEDDDBDDDD;BDDBBDDDCDDDDDDBCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18890:1897 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIIJJJJJJJIJJIJIHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:1102:2244 +CCACGTCTAGCAGGAAGATCGGAAGAGCGTCGT ++ +IHIIHHGIIH@FGDFIIGJHJJGIIGJHGEFD> +@HWI-ST575:165:C1P5BACXX:1:1101:2310:2103 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +BADHGGCCCHHGIHIIH;;44=BAB8;/886855@77<<+>CBDDDDB?:3> +@HWI-ST575:165:C1P5BACXX:1:1101:3289:2146 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTCCCGCGTTCCACTCTCAC ++ +IGGFGGGIEGIIJGHGIGJB:BBB>>88<==?@BDBDBD(>CBDDB@?<CCDCDACD +@HWI-ST575:165:C1P5BACXX:1:1101:4895:2080 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JIJIJJJJIJIJJGIJIIGHHFFDBBDBDDDBBDDDDBDDCDD;<DDDDEDDDCCDDDDACDEE:44+,(44+4@? +@HWI-ST575:165:C1P5BACXX:1:1101:4932:2108 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCTTGATAAG ++ +FFHA?D=G<FBG>FCH8AAEB<63;@??B@B8<BBDBD@9C37B7@BDDEDD +@HWI-ST575:165:C1P5BACXX:1:1101:5806:2073 +CCGTCTGATGGTACAGATCGGAAGAGCGTCGT ++ +C;6@@F1<DDHCIIG@G<CF6@7ACCGEHEBC +@HWI-ST575:165:C1P5BACXX:1:1101:6214:2065 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +HJIGHJJIIJJJJIJJJB@EHFFDDDBDDDDBDDDDDDDDCCDDDDDD9>ACDDCC +@HWI-ST575:165:C1P5BACXX:1:1101:6143:2137 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GGD0??)BBB8BBGHFGGHABFB<@?B;<@B=8-<B@@@ACCBDDDDBDDDDCACCA +@HWI-ST575:165:C1P5BACXX:1:1101:6241:2199 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIGEHI?HIIIGJJJIJIIEFFFDB?BBDDDDDBDBDBDDDDDDDDDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7894:2030 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HJIJJJIJJJIJIJIIIFEGHHFFDDDDDDDDDDDD:BDDDDDDBB?CEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8072:2245 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIGIIFJIIJJJIJJJIJHHEFDDDDBDDDDDDDDDDDDDCD@DDDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8299:2087 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJIJJJJJJFIJIIIHHFFDDDDDDDDDDDDBDDDDDDD;BDDBCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8471:2183 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIIIJJJJJJJJJJJIJJHHFDDDDDDDDDDDDDBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9051:2011 +CCGATTTGGATCTGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGA ++ +IIGEHICIIIJJJJIJIIJJJHHFFDDDDDDDDDDD@<BCDDDDDDDDDDCDDDDDDDDACDEE4,,,4 +@HWI-ST575:165:C1P5BACXX:1:1101:11298:2099 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJIJJIIIJIIJIJJIJIHHFFDDDDDDDDDDDDDDDDDDCDDBDDDDECDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11886:2227 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCAC ++ +IGH@FE=GGEGGGGGGIIIEEFBCB?B?<==?BDBBDBBCDDDDDDDBDED +@HWI-ST575:165:C1P5BACXX:1:1101:12469:2156 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJIJJJIJJJJJJHHFEDDDDDDDDDBBDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13307:2211 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHHIJJIHIIIJIJIJIHHFFDDDDDBBDDDDD@BDDCCDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13967:2112 +CCGATGTGGATCCGCAGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +1:?0?))09*09/?<@F@F@9BB/=339,38'3,7<B5<3A(0;B<<<CD:A<>AC:?A(4(4>4(++,+:4+4:? +@HWI-ST575:165:C1P5BACXX:1:1101:14357:2064 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJHHJIJJJJJJJJJJJIGHFFDDDDDDDDBDDDDBBDDDDDDDDDDDEDDDDDDDDDCCCCD4(++4+: +@HWI-ST575:165:C1P5BACXX:1:1101:14498:2106 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJIJJJJIJJJJIJJIJJHHFFDDDDDDDDDDDDDDDDDDCDDDDDDDEDDDDDDDDDCDDEE4+++++4 +@HWI-ST575:165:C1P5BACXX:1:1101:14632:2166 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJHIJJJIJJJJIJJJJIHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDBDCCDEA4(,+++:4:>AB +@HWI-ST575:165:C1P5BACXX:1:1101:14873:2011 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJGIJJJJJJJJJJJJJJHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDDDCCDDE4+,,4+::4@C? +@HWI-ST575:165:C1P5BACXX:1:1101:15054:2152 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJHGICEGGGIJFFIGIIGHFFDDBDBBBDDDDDDBBBCDDDDDDDDDDDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:15855:2031 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCA ++ +IJJIGGIGIIHGIIIGGHGFFFFDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15913:2171 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCT ++ +III:1D?F@<<DA;FG:ABA;EEEBBE<59;8?BBDD@BCCDB;BB@BA@CCCC +@HWI-ST575:165:C1P5BACXX:1:1101:16436:2126 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +HGG@?BDFCDFBAFBBFGG:=DEC@9>;?=?'05<BDBBACC@DDDB?CE@CCA:A +@HWI-ST575:165:C1P5BACXX:1:1101:16535:2143 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +CFE0@BBFDG<F3CHCF@GB<B<3>89;8==279@BB8?CDDDDDDDBDECDDCCDD +@HWI-ST575:165:C1P5BACXX:1:1101:16829:2249 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIIHEGGHIGIIIIIGIJIFFFFDBBBDBDD357B<BDDDDDDDDDDDDEDDDCCCD +@HWI-ST575:165:C1P5BACXX:1:1101:17455:2003 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIJJJIIJJJJIJIJJHHFFDB@DDDDDBBDDDDDDDCCDBDDDDDEDDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17878:2158 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIJJJJJIJIIIJIJIGHFFDDDDDDDD?BDDDDDDD:@B<@DDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18041:2200 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCACGTTCCACTCTCAC ++ +JJJIJJJJJJJJIIJJIJJHHFFDDDDDDDD=BDDBBDDDCDCCDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:2284:2369 +CCGGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GGE<DFBFABGEHEEGGAEABE@C?;8;8?@8:@85:<<3>A875@;?9>@CCDCDC +@HWI-ST575:165:C1P5BACXX:1:1101:2836:2439 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJJJJJIJJJIIJJJHEEEHFFDDDDDDDDDDDDDDBD9ACDBDDDDDEDDDDDDDDDDDEDD:++44+:44(42 +@HWI-ST575:165:C1P5BACXX:1:1101:4292:2260 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJIIIJJJJJIIIJJJHHFDDD@DBBBD;@DDDBBDDCCDDDDDB?>CDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:4629:2330 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GEHGBDBFGGCAFGEEGBGEB>BC??BB?@B6555:57??@CDB@<@B?:>C?ACDC +@HWI-ST575:165:C1P5BACXX:1:1101:4537:2454 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJIHBHGGGIJJJIIJGJIHHFFDDD@=BDB@BDBBDDDCDCDDBDDDDEDDDDDDDBCCCDEE:+,+,+:4+4@? +@HWI-ST575:165:C1P5BACXX:1:1101:5316:2437 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJHIIGIFIJJJGJJJFIEGHGFFCDDDDDDDDDDB7BCCCDDDDDDDCDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5705:2286 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJIJJJIJJJHHFFDDBBD?BB@DDDBBBDCCDBDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6181:2354 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJGIJIJJJJJJJJJJGHHHFFDDBDDDDDBDDDDBBDDDDDDDDDDDEDDDDDADDDCCDDC:++,4+4+,4>? +@HWI-ST575:165:C1P5BACXX:1:1101:6640:2254 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +JJJIJIGIIJJJIIIIIDIHHFDDB@@@?B@?98B@5:BD@CDBDDBDCDCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6520:2397 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJIJJJJJJJJJJJJGHFFDDBBBDDDDDDDDDDDDDDDDDDDBDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7019:2438 +CCGCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTC ++ +JJJJJJIJJJIJIJJIJJHFFFDDDDDDDDDDDDDD:BDCADDDD@<CDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8048:2260 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIFHIIJIJIJJJIJJGGFHFFDDDBBBDDDD@D@BDDDDCBDDDDBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8522:2464 +CGGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIJJJJJJJJIJJJBHGHFDDDDDBDDDBDDDDDDDDDDDDDDDDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8845:2462 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJJIJJJJIJGGFFDDDDDDDD8BDDBDDDDDDDDDDDBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9068:2302 +CCGCTTTGGATCCGCCGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACC ++ +IJIJJGGIIJJIJGHIBHHFDDBDDDDDDBB8B9@?CDCDDBBB@<@CDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11110:2418 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJIJIIJJJJJIIJIHHFFDBDBDDDD=BDDBDBDACDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11986:2397 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCT ++ +JJGGGIGIJIJEHIGIIJIGEFFCB?@88?B=BBD<<BBDDDDDDDDBDDC@?C +@HWI-ST575:165:C1P5BACXX:1:1101:12227:2444 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIBHICIGGGIIJJIIIIFFFFDDBD@@DDDDDDBBBDDDCDDDDDDDEDDCCCCD +@HWI-ST575:165:C1P5BACXX:1:1101:12315:2285 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIGGHIIJIJJGJGJJGGGHHFFC@?B@BDBBBBBDDBBDACD;BDBBDDCC<CCDD +@HWI-ST575:165:C1P5BACXX:1:1101:12492:2350 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCA ++ +JJJGGJEGJJIJJJJJJIJGDFFDDBDBBDDDDDDBDBDDDDDDDDDDDEDDDDCDD<A +@HWI-ST575:165:C1P5BACXX:1:1101:13583:2475 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHIJIJIJJJJJJJJJJJGHFFDDDDDDDBDDDBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14469:2354 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCACACACTCT ++ +JIJIIIIIICEFGHIIJJIHFDFDDBD@?BBBBDB<9<DCCCDB&07<?B99@ +@HWI-ST575:165:C1P5BACXX:1:1101:14989:2278 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJIIIJJJJJJJIIJJJJJGHFFDDDDDDDDDDDDBDDDDDDDDDDDDDEDDDDDDDBDCCDEE4+,,,+4 +@HWI-ST575:165:C1P5BACXX:1:1101:15188:2338 +CCGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAG ++ +JJJJJJJJJJJJJJJIJJJHHFDDDDDDDDDDDDDD@BDDDDDDDBBCEDDDDDDDDDDDEEE>4444( +@HWI-ST575:165:C1P5BACXX:1:1101:15331:2257 +CGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJJJJJJJGGIHHFDDDDDDDDBDDDD<BDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15849:2368 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJIJJJJJJJIJIJJIIIIJJHHEFDDDDDDDDDBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:16152:2410 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJIJJGIJJJJJIJJIJIEEBDDBBBDDBDDDDDB59BDDDDDDDDBCEDDDDDDDDD:>CCD4+++++4 +@HWI-ST575:165:C1P5BACXX:1:1101:16935:2365 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJIIJJJIJGIJIJJGHFEDB@BBBDD6<BDBBBDDDCDBDDDDDEDDDDCCC +@HWI-ST575:165:C1P5BACXX:1:1101:17289:2412 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCACGTTCCACTCTCAC ++ +IIJGDHIGGEHIJIIJJJJHFFFDDBDDDDD@DDDDDDDD:@@@BDDDDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:18384:2478 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJHJJJIJJJJIIJJIGIHHFFDDDDDDDD=@DDB<BDCCDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19068:2414 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +DFDEHIIJJJIJGGIIIJGHFFDDCDD<BBBDDDD57?C@CB@DDD@DDDDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19461:2478 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJJIJJJJJJJIJJJJIJIGHFFDD@DDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDDDCCDEE:+444+:44:A@ +@HWI-ST575:165:C1P5BACXX:1:1101:19502:2351 +CCGGTTTGGAGCGGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +8?@F@)0??9).'-5-95=<'962;'372-57:5BB8@<>A:9B<DB8CD3@ACCD +@HWI-ST575:165:C1P5BACXX:1:1101:19741:2372 +CCGTCTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJGIIJIGHIJEDGE/@B<A><=@BD@BB?BDDD8:@CCCDB<5;8ACCCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19565:2447 +CCGTTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJFHJJJJJJJJJJIIJJHHFFDDDDDDDDDDDDDDDDDDCDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20585:2479 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJJHIJJJIIIJIIJGHIGFHFFCDDDDDDDDDDDB@@DDDCDDDDDDDEDDDDDDCBCCDEEE:4444+:+,+:? +@HWI-ST575:165:C1P5BACXX:1:1101:20888:2259 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +BHIGGHAHG>B9BFHGFHGAE6?A>?BD??D=@BD905@9+37BBBBB?>CAC>@(: +@HWI-ST575:165:C1P5BACXX:1:1101:1310:2696 +CCGTCTGGATCCGCCGAAGCGGA ++ +IJIIGI=FFHHFGGGGGGGEEED +@HWI-ST575:165:C1P5BACXX:1:1101:4969:2645 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GGIGIIJEIGHGHGHIJFGHHFFCBBBDDBDBDDDBBBD?CCDDDDDDCDDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:6485:2694 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJHIJJJIJIJJJJJJJJHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDCDDDDDDCCADDD4+,44(4+44:8 +@HWI-ST575:165:C1P5BACXX:1:1101:6896:2699 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIDHJGGJGHDFHIIG8AEEFE<@BDDDDDB-75<<B@>:>55<BDDDECCCCCCC +@HWI-ST575:165:C1P5BACXX:1:1101:7108:2513 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIJJJJJJJJJJJJJGGHHFFDDBDDDDDDDDDDDDDDDDD@DDDDDEDDDDDDDDDCCDEE:+444(4++:>? +@HWI-ST575:165:C1P5BACXX:1:1101:7123:2615 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJIIGJJJJIGHFFDDDDDDDDDDBBD@BDDDDDDDDDD?DDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7199:2618 +CCGATTTGGATCCGCCTAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJIDIIJJJIIHJIJJGGIGHFFDDDDDDDDDDBBDCDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10015:2714 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJGHHIJJJJJJJJJJIJIHHFFDDDDDDDDBDBDDBBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10105:2714 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIIGIJJJJJIGJGIJGGFFDDDDDBDDBDDDB<BDCDDDDDDDDCEDDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:11341:2736 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJIJJJJIJJJJJIHHFFDDDDDDDDDDDDDDDDDCDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11611:2512 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJJJIJJIJGHGFFDDDDDDDDDDDDDBBDDDDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11611:2576 +CCGATTTGGATCCGCCAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +IJJJJJJJIJJJJGIJIIIIIHHFFDDDDDDDDDDD@DDCCDDDDD?CEDDDDDDDDDCCEDE:4444+: +@HWI-ST575:165:C1P5BACXX:1:1101:11522:2633 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJIJJJJIJIJJIJJJJIHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDDCDDEEB4+4:44: +@HWI-ST575:165:C1P5BACXX:1:1101:12762:2599 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJBGIJI@FGEHIIJEBHEHFBCDB@??@BD@BDBBBDCCCBDB<;@CACCCCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13122:2666 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIJJJJJJJIIJJIJJHHFFDDDDDDDDBDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13331:2504 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGHIIIJIIIIJJJJIJHHFFDBBDDDDDDDDDDDDDDDDDDDDD?CDDDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:13328:2614 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGIIJIIHJJJFGIG>HEHFFDDBBB@DDDDDDBBDDCCDDDDDD@DDDDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:13944:2746 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAG ++ +IJJIJJIJIIJJJJIJIIIHHFFDDDDDDDDDDDDB@BDCDDD@DDDDDEDDDDDDDDDCCDE@4(++4+ +@HWI-ST575:165:C1P5BACXX:1:1101:14544:2676 +CCGATTTGGATCCGCAGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTG ++ +JJJJJJJJJIJJJIJJJJJIJJHHFDDDDDDDDDDDDDDDDCDDDDDDDEDDDDDDDDDCCDED:(+, +@HWI-ST575:165:C1P5BACXX:1:1101:16211:2706 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +?CG?GBBGGHCHDHIHAAAEHFDCBDBDDDDDBBB@@BACCDDBB@@CDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17293:2515 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJEHIJJJJJIIJJJJJJHHFFDDD;@BDD@DDDB@BDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17341:2572 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +@FG:@D?GFDD<BDFCFEH>BFE??;??@BB/75@<BBBDDDBDBDD@CDCCDCCCD +@HWI-ST575:165:C1P5BACXX:1:1101:17813:2676 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +EHG@FGGGGGC@BGFEBBA=EB<>>;;8?@@'55875:?CCCDDBDDD?@CCACC@C +@HWI-ST575:165:C1P5BACXX:1:1101:18322:2589 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIJIGJIGEGIIJIIIGGDFDDDDDDDD=:BDBBDDDDCDDDDDDCDDDCDCDC +@HWI-ST575:165:C1P5BACXX:1:1101:18563:2613 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJIJJIJJJIJJHHFFDDDDDDDD8@BD@BDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19030:2538 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +DFD)888BF?8??FGBF@G84@<<@>?;,888:@@::98>C@@DDD<<ACAC9>ACA +@HWI-ST575:165:C1P5BACXX:1:1101:19375:2502 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIIGGFIJIGHJIIIIJJGEHFFDDDDDDDDDDDDDBBBDCDDDDDDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19630:2588 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +;FH?F@GHIHBD3CHAFBHGHEFCDBDD?BBDDDDB@@?CDDDDBDDBDEDDDDDDDDDDDEED:4444+:+++48 +@HWI-ST575:165:C1P5BACXX:1:1101:1885:2921 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIJBHIEHGIIIJGJJIHEAHFDCD@>@?BB@BB@BBBD?CCDDDDBB?DCDDCCC@ +@HWI-ST575:165:C1P5BACXX:1:1101:3712:2816 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIIJJJIJIJJJJJJJJIHHFFDDDDDDDD@BBD@B@DDDDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:3931:2939 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +DFIEH@<GAFDFHFCHBEABBFDABDD@?@?555<05?>:CDD;7;@93>C?CDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4575:2908 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +EGIHGEEGHHGGEHGGAAGHEEE?BBB8<?B;@BD@BDDDC>@@BDDDDEDDDDC@A +@HWI-ST575:165:C1P5BACXX:1:1101:4670:2972 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIIJIIJJJJJJJJIJIHGFFDDDDDDDDDDDDDDDDDCCBDDDDDDEDDDDDDDBCCDEEE:4++,,:4+4>8 +@HWI-ST575:165:C1P5BACXX:1:1101:4779:2823 +GATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCGTC ++ +JIIIIIJJJJIIJJJIEGIHHFDDDDDDDBDDDDB:@CDDDDDDBDDEDDDDDDDDDDDEEE:++44+4++4><?@ +@HWI-ST575:165:C1P5BACXX:1:1101:4804:2998 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHJJJJJIJJJJJJJJJHHFFDDDDDDDDDDDBBDBDDDDDDDDDDDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:5202:2792 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJGIIGI@DHGIJJEHGGFHFFDDDDDDDD@BBDBBBDCDDDBDDDDDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:5939:2936 +CCGATTTGGATCCGCCGAAGCGGATAGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJIIJJIIJJIJIIJJIGIHHFFDBBCDCDDDDBDBDDDCCCDBDDDDCEDDDDDDDDDCDEEE:44+,(4++4:? +@HWI-ST575:165:C1P5BACXX:1:1101:6091:2918 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHIJJJJJJJJIJJJIIHHFFDDDDDDDDDDDDBDDDDCDBBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6392:2828 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJIJJJIJJJGJJIJJJIGHHFFDDDDDDDBBDDDBBDDDDDD<BDDBDEDDDDDDDDDCCDEE:+,44(4++4>? +@HWI-ST575:165:C1P5BACXX:1:1101:7180:2991 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJIFIJIJJJJIJJJJJGIHHFFDDDDDDDDDDDDBBBDDDDDCDDDDDEDDDDDDDDDCADEE:+4:4+4++4>? +@HWI-ST575:165:C1P5BACXX:1:1101:7419:2920 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGIIJJIIIJIIJJGIIFHFFDDDDDBDDBDBDBBBDDDDDBDDDDDEDCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7772:2979 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HIHIJJIJIIJJIJJJJJHHHFDDDDDDDDDDDDDD:BDDCDDDD@<CDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8507:2811 +CCGTTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HIIHIJIJIEIJIGIIJIIGHFFDDDDBBBDDBBDBBDDDDDBBDDDBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8832:2869 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHIJJJJIJJIJJJJJIGHFFDDDDDDDD?BDDDDDDDDDDDDDDDDEDDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9485:2898 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJFDHHJIJGIJJIIJEHGBEFECDDDD?BD=BDD@5:BC@CDDDDDBDCDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9990:2821 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIIEHIIJJGIGJJJJGIIEHFFDDDDDDDD8@<BBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10328:2966 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJJIJJJJJJJJJJJJJJHHFFDDDDDDDDBDDD@@BDDDDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11508:2796 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJIJJJJJIJJJJIHHFFDDDDDBBDDDDDDDDDDDDDDDDDBCECDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11726:2972 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJA:@GH<BB@B@BBCAA8<AD68??@88?@8550507?>CC5@;;7B<@@C?C@C> +@HWI-ST575:165:C1P5BACXX:1:1101:12044:2834 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIJJJJJJJJJJIJJJJJFHFFDDBDDBDDDDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12169:2963 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GJJGIIJI*BFIJJJJHIIHHFFDDDDDBDDDDDDBBBDCDDDDDDDBDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:13168:2760 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIFHIJJJJJJJJJJIIIGHFFDDDDDDDDDDDDBDBDCCDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13484:2768 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIGHGGIJJGIIIJJJJGJGGFFCBBD?BDDBDDDDDDDCCDDDDD@?CDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13399:2774 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJGIJJJJJJIJJJJIGIHHHFFDDDDDDDDDDDDD:BCCCDBDDDBCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13467:2781 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IHIDHGGIJGIIJJJJIJJIHHFFDDDBDDDBDDDBDDDCDDDDDDD?CECCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13716:2837 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCCGTTCCACTCTCAC ++ +JJJIJJJJIIIJIIIIIJJGHFFDDDBD?BDDDDDDDDDDDDDBBBDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14400:2843 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJIJJJFIIJIIJFIJIGIHHFFDDDDDBDDDD8BB<BBDDDDBDDDDDEDDDDDDDDDCACDC4(+,++4 +@HWI-ST575:165:C1P5BACXX:1:1101:14816:2802 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JIIHIJIJJIJHJIJJJJIHHFFDDDDDDDD=BBDB<BDDDDDBDDDDDEDDDDDDDDDCCDDE:+++4+4 +@HWI-ST575:165:C1P5BACXX:1:1101:15055:2926 +CCGGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +FHIG?6:DDBGG3.1@;;AE<?6/;3,;@BB555<<00<9@@8705<<A@AA +@HWI-ST575:165:C1P5BACXX:1:1101:17639:2908 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCGT ++ +JJIJJJJJJJIJJJJJGHHEFFDDDDDDDDDDDDDDDDDCCDDDDD@CEDDDDDDDDDDDEEE4++444:+,+:<C +@HWI-ST575:165:C1P5BACXX:1:1101:20319:2983 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJJGGGIJIHIJCGGIIDGEHFFDD@BBBDDDDDDB@BDDDDDBDDDDDEDDDCDDDDC>:CDD:+++4+:,+:>@ +@HWI-ST575:165:C1P5BACXX:1:1101:20783:2769 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGAGTTCCACTCTCAC ++ +IJJGIJJIJIJJIGIIIGIHHFFDDDDDDDDDDDDDDBBCCDB@DDBC:ADDD@CDD +@HWI-ST575:165:C1P5BACXX:1:1101:1673:3017 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +?FGDGH0BDFD?B2BBF<E6:?B<?8;=<@=/0077@8?(3:07<BB8?DC:9A>A +@HWI-ST575:165:C1P5BACXX:1:1101:2153:3048 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAAACTTCG ++ +;D<0@B=DE<F@BG6;FGG/AD8>@'9?(8;'55<5<@@A@:7&5;B?CCDC?A>::2>::>AC:+4+,(4+++:< +@HWI-ST575:165:C1P5BACXX:1:1101:4715:3184 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJIJJGGGHI>GGHGGGGEEFFCDDDBBDB/59B9BBDADC@BDDDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4796:3234 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +DHIJJGEHIIIGGCGHEGIHHFFDDB?BBDD@BB@:7@BCDDDBDBBDA@CCCCC:A +@HWI-ST575:165:C1P5BACXX:1:1101:5104:3008 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJIJGIJJJJJIJJGIJJJJHHFFDDDDD@DDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5272:3008 +CCGGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJDEDGHIJIJIFIIJHIEEFD?BDDDBDDDDDDD@@BBDDDDDDDBDD@CDDDCDC +@HWI-ST575:165:C1P5BACXX:1:1101:5594:3119 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +CBFEH=BFAGCB)8;@;;AHFDF>>??=58=2:8557@?3@>@BDBDDD3AC +@HWI-ST575:165:C1P5BACXX:1:1101:6927:3052 +CCGGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +DGGEIIGIGGBGF;CGIGGE6BDCCB>BB<@D0<B9B55?AA@B;;@B?CD@C?>CCC +@HWI-ST575:165:C1P5BACXX:1:1101:7098:3130 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJHIJJJJJJJJIJJIIIHHFFDDDDDDDDDDDDDBBDDDDDDDDDDCEDDDDDCDDDCCDEC4(,44+4++4>? +@HWI-ST575:165:C1P5BACXX:1:1101:7842:3018 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JIIIGIGIJGIJIIJJIIIHHFFDDDDBDDDDD@B@@BDDCCBDDDDDCDDDDDDCDDC>>CD>4+,+4+4444:8 +@HWI-ST575:165:C1P5BACXX:1:1101:8316:3056 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIJGI?FHEIJJIGJIIIGHFFDDDDDDDDDBBDDDDDDCCDBDDBDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8582:3236 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +EIBB@BBGIIIEHIEBAEAEHFB9;>>8?BDDD8BBD8@CDDDDDDDDDEDDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:10343:3076 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJJJIJJJJJHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10433:3184 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGG ++ +JIJIJJIJJJGJIIIJJJJHHFFDDDDDDDD=BDDB +@HWI-ST575:165:C1P5BACXX:1:1101:10787:3078 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIDFHGGEFGGHIJJIIGHHFFDBB>B?@B=@BB<@BBCCDDBDBDBDDCCDCDDC +@HWI-ST575:165:C1P5BACXX:1:1101:10951:3116 +CCGATTTGGATCCGCCGAAACGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJGHIGGIJGGIJICHGGIHHFFFDDDBDDDBBDDDDDDDDCD;BBBDCDDDDDCCD +@HWI-ST575:165:C1P5BACXX:1:1101:11196:3016 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJHGIIJIJJIIGGIAGIGHFFD/;BDBDD@BDDB@BDCCDD@DDD@CDDDCDCCC +@HWI-ST575:165:C1P5BACXX:1:1101:11257:3175 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGIIJIJJJJIIJJJIJHHFFDDDDDDDDDDDDBDDDDDDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12061:3148 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJGGGI@FBBGHIJJJJJJGIJGGFFDCA@D:B:B<BBDCDCBDDDDDDECDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12768:3208 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJHIJJJJJJJJJJJJGIIHHFDDDDDDDDDDDDDD:BCDCDDBDDBCEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14168:3032 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHIJJJJJJJJIGIJIIGHDEDDBDDDBBDDDDD89BACDDBDDDDDDCDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14033:3072 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +?DF@DDGHGDDFBGIIG8EB:BBBB8;?5=B=B8::57<9CC@BDDD?CDDDCCCCA +@HWI-ST575:165:C1P5BACXX:1:1101:14598:3012 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJG@@FHIIEDDGHGEHGIAEEFDDBDDDDD?B@B9<BDCDCBDDDD?CDDCDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14516:3084 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJGII=FGGIGIIGIEGHHFFFDDDBDBBDB@BB@BBBCDDD<DDDDACCCDDCCC +@HWI-ST575:165:C1P5BACXX:1:1101:15426:3038 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +GIFHIIJJJJJIJIJEHEGIGEFFDDBDDDDDDDDDBBDDCDDDDDBDEDDDDDDCBDDCDDD:++,4+: +@HWI-ST575:165:C1P5BACXX:1:1101:16490:3172 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIJJIJJJJIJJJJJJHHFFABDDDDDD=BDDBBBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:16810:3009 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJGEIJJJIIJIIIIJJIIFHFFDDDDDDDD;BDD@<BDDCDD@BDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17555:3239 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHJJIJJJJJJJJJIJIHHFFDDDDDDDDBDDD@BDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18319:3009 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJGIJJJIJGICGGHGHIGHFFDDBBDBDD;BBD@BBDDDCD@BDDDDEDCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19255:3046 +CGTCGTCTGTGAGATCGGAAGAGCGCGT ++ +GIHIIGFFHHICHGIIJJJJGIEGHGDA +@HWI-ST575:165:C1P5BACXX:1:1101:20168:3013 +CCGGATTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJIGJIJJJIEHGHHHFFDDDBDDDDDDDDDDDDDCCCDDDDDDCEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:21025:3228 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GIJIIIJJF@BDGHJ@A@EHABBA@BDD8?BBDDD770<CCCBDBB;?CCDDCCDC@ +@HWI-ST575:165:C1P5BACXX:1:1101:3940:3277 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJGJJJJJIIJJIGIHHFFDDDDDDDDDDDDBDDDDDDDDDDDDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5732:3431 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTA ++ +IJJGIIJGIJIJIJIHIGIFGFFDDDDBBDD6<<BBD@?A +@HWI-ST575:165:C1P5BACXX:1:1101:6498:3306 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTTC ++ +JJIJJJJJIJJJJIJJJJIHHFFDDDDDDDDDDDDBDDDDDDDDDDDDDEDDDDDDDDCDCDEE:,+:4+:+++:@ +@HWI-ST575:165:C1P5BACXX:1:1101:6927:3383 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIJJJJJJJJJJJJJJJGHFFDDBDDDDDDDDD@BDDDDDDDDDDDDEDDDDDDDDDCCDEE:+,44(4+,4:? +@HWI-ST575:165:C1P5BACXX:1:1101:8668:3366 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGAGTTCCACTCTCAC ++ +JJJGIJGEEHHJJEHJ>EEFHBEBBABCBBD;BB@<9:B9@CB&5?BCCDCCCCACC +@HWI-ST575:165:C1P5BACXX:1:1101:9555:3271 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIIGEEDDCGGIIBFHEHHHHFDCBD@B?BD?9BB<9@BCCC@BBDBBCCCDADDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9960:3491 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCT ++ +JJJEHIJIJIGGIJJIIJIFHFFDDD@BDDD?BDDDB@BDCA<@BBBBCEDA?> +@HWI-ST575:165:C1P5BACXX:1:1101:11371:3324 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIJJJJIJJJJIIJJIJJHHFFDDDDDDDDDDDDDDDDDDDBDDDDDDECDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:11999:3288 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIGHEHJEIHGIIIJJEIIEHFDDDDDBBDDDBBB<@BDCCDDB;BBBDECCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12147:3289 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJIIIGIJJJIHHFFDDDDDDDDDDDDDBBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12666:3312 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHJJIJJJJJJJJJJJIGHFFDDDDDBDDDDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12701:3456 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJIGHJJJJJFJIFHFFDDDDDBBDDDDDDDDDCDDDDDDDDCDDDCDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:13062:3317 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HGIJIIIJEIEGADGHG@GHFDFA@>;@<?B?B<<<@<B?CCDDDDBDCDDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:13545:3337 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJJJJJIJIIJJIJJJJIHHFFDDDB?BDDBD@DDDDDDDDBDDDDDCDDDDDDDDDCCCEED:4444+: +@HWI-ST575:165:C1P5BACXX:1:1101:14345:3429 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +IJIHIJJIJIGJIIJJGJIGHFFDDBBDDDDDDDDDBBDDCDDDDDDBDEDDDDCDDDCCCEDD4+++,+4 +@HWI-ST575:165:C1P5BACXX:1:1101:14585:3422 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +IIIJIJJJIIIJJIIJIGIHHFFDDDCDDDDDDDDDDBDDDDDBDDDDDDCDDDCDDDDCCEEE4+,+++4 +@HWI-ST575:165:C1P5BACXX:1:1101:16628:3381 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJGGIIJJJJIFJJJJIGGHFFDBBBDDDD;BDDDDDDDDCDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:1080:3578 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJJBHIJJIEGJJIJJJIIHGFFDDBBD@BDD@@DDDBD9AA@<D;BDCCCDDDDDCDDCCAAA44+4:4>:4+:< +@HWI-ST575:165:C1P5BACXX:1:1101:1525:3749 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +GIIIIJCHIIIIIIJJJIIEHFFDD@DBBDDDDBDBDDD3@A9<BDDBDECDDDDDDDDDCCDD4++44+:44::? +@HWI-ST575:165:C1P5BACXX:1:1101:2431:3630 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJIHEH;DGHIGJJIJIJGBEFEDD@BBDDDDD<@9BDDADDBBDDDDDEDDCDDDDDD@>CDA4,++,+444+42 +@HWI-ST575:165:C1P5BACXX:1:1101:2802:3618 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIFIJJGJJIJJJGJJJJJJIHFDFCCDDBDDDBDDD?CDDBDBDDDDDDDDDDDDCA>CCD4(,+++4:44@< +@HWI-ST575:165:C1P5BACXX:1:1101:3064:3520 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJHIJJFIJJJIJJJIJIHGEFDDDDDDDDDDDDDB7BCDDDDDDBDCEDDDDD@C +@HWI-ST575:165:C1P5BACXX:1:1101:3610:3614 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +IJDHJJFGHIJIEGIJGIIGBFBC@BBBBDD=@BB<@<BACDD@BDBD9>CD?CCC +@HWI-ST575:165:C1P5BACXX:1:1101:4225:3617 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIDGIECBHIDHJIIEHGIFEFFDD@B?BDD?BDB<B@@CCDDDDDBB?CCCCCCCC +@HWI-ST575:165:C1P5BACXX:1:1101:4165:3628 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCAGTCTCAC ++ +IJIGGGCDECHGICGIIGIHHFFCDDDDD/=B7B@@<BDCC@BBBDDDDEA>ACDED +@HWI-ST575:165:C1P5BACXX:1:1101:4951:3655 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +HIIDHE7DBCDHG@BFHGBH<BBBBDDB8@B'5587BBBDDDBBB;@D<@>A +@HWI-ST575:165:C1P5BACXX:1:1101:4878:3725 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJIJJJJIJJJHIIHHFFDDDDDDDD?BDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5530:3596 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIGIIEIJIIJJJJIJJIFHFFABDDDDDD@DDDBDBBCDD@BDDDDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6120:3600 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCAC ++ +HIHB>EC@HHBFEBEF@4@6ADB>BBBB8?;?BDD<75??AC575;@8CDD +@HWI-ST575:165:C1P5BACXX:1:1101:6900:3547 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIEGHIJIJJJJJJJJIIGHFFCBBBDDDDDDDDBDDDDDDDDDDDBDDDDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7633:3566 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +DHEGGE7FCFEGEHGIH8BA:BDA@;BBB=@B@1<:<@<?CCBDDDDBCDDDCCCDC +@HWI-ST575:165:C1P5BACXX:1:1101:9213:3506 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +3DGGIJGIIIIJJJJIJGIFHFFDDDDBDDD6<@DBDDDCDDBDDDDDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10414:3701 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHAGEHIIHHIIIJJJJGFFFDDBDDBDDBBDBDBBDCDDDDBDDD?CDDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10901:3561 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +JJJDGIJIJIIJJIJJIJIFFFFDDDDDDDD8:BDD<@DDDDDDDDBDDDCACACC +@HWI-ST575:165:C1P5BACXX:1:1101:10915:3580 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIGHHGHIJJJJJJJJJIGHFFDDDDDDDDBDDDDBBDDDDBDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11184:3661 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCAC ++ +FD::??DHC??BFGGCHBHB6B69;9;8538;77<@BB?9CC@BDDD?CD@ +@HWI-ST575:165:C1P5BACXX:1:1101:11415:3524 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJFHIJCIIJIIJGII8FHIHHFDAB?BBD8=BDD@7@CCDDBDD@BDEDD?ACDC +@HWI-ST575:165:C1P5BACXX:1:1101:12086:3581 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJIJJIHJJJJJJJJIJIFHFFDDDDBDDDDDDDDDBDDDDDDDDBDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12420:3632 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +FHDF@FGHGDGH>FDDHBH84=BDBCAB=@BD?=<8@8@ACCDDDDB?CDDDDADDA +@HWI-ST575:165:C1P5BACXX:1:1101:13315:3747 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +BF<G@@B9?9?F?=BHB8AB89639@;/,3=;:@B5@BB33>5)07@03>C><:AC +@HWI-ST575:165:C1P5BACXX:1:1101:14374:3673 +CCGGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJJGIGJJJJJJIJJIIGGFFDDDDDDDDDDDDDD@BBCDDDDDDDDDEDDDDDDDDD@CCCD4+++++4 +@HWI-ST575:165:C1P5BACXX:1:1101:14502:3661 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JIGDHIGJIGIIIIIIIJIEHFEDDDDBBDDDBDDB<BDDDDDDDDDDCDCDDDDDDDDCCCDE4+++,+4 +@HWI-ST575:165:C1P5BACXX:1:1101:14952:3691 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +?GG<DF@GHGCHHGI?G@EEHFFDD@DD8@B@DDDBD@B?CCDDDDDDDECDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15058:3596 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGIJJJJIJJJEGIGIIHHFFDDDDDDDDBDDDB@BDDDDDDDDDDCEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17467:3686 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJGIJJGJJGIIJJJIIJHHFFDBBBDDDDBDDDDDDDDDDDDDDDDADDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17669:3607 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIJJJJJJJIJJJJJJJHHFFDDDDDDD=BDDBBDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18356:3623 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJFHIJJIJIIJIIJIIIHHFFDDDDDDDD@BDD@@BDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18913:3633 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCT ++ +JJJIIJJIIJIJIJJJIIJHHEFDDBBDBDDDDDDDDDDDDCDDDDDDCEDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20863:3620 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +BDHDB@FIIDHCGHIGIHFHGFECD@BB?BB=5BB@<@BDCABDDDD@DECDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:1773:3939 +CCGTTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJJJJJGIJJIIJJJIJJGADDBA@BDBDDBBDDBDDD>CCDBDDDDDEDDDDDDCDD>@CDE:+++4+:44(:< +@HWI-ST575:165:C1P5BACXX:1:1101:2081:3878 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTG ++ +JJIFHIFHIJJJJGIJIJJHHFFDDDDB@DDBBDDBDDD93:9B7BDDDEDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:4430:3860 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HIEHIIDCGGIJIGIIBHIHHFFDDDDDDDDDDDD@<:@CDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6400:3801 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJIJIJJJJJJIIGGHFFDDBDDBDDDDDDBDDDCCDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6719:3898 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIIJIGIGIJJJEGGGJIHGFFDDDDDDDDBDDB@BBDDDDDBDDDDDEDDDDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:8167:3757 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HHGGBG7GCFHIEEGEG<EEE>B6>BB?<?;=5@B<@<BACCBDDDDDDEDDDC>AC +@HWI-ST575:165:C1P5BACXX:1:1101:9714:3894 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIIBBDGHGGIIIGGGIJIHHFBCBDBBBDD?<@BB99BCCDBBDDDDCCCDDDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:11290:3835 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIIIJJIJIJJIJJJJIJHHFFDD@BDDDDDDDDBDDDCDCDDDDDBCEDDDDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:12038:3902 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IGHGIEGHGGGIIGEHEHIGHFFDDBDDDDDBB8B<5:BCDDDDBDDBCCADDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12700:3779 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJIIIJJGJJHHFFDDDDDBDDDDDDB<BBDDDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12790:3907 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +BFG@@FFGCBDGGIFGIGGABHE>AAA@=@?8@BD@BDBACCBDDB;BCDCCCCCDD +@HWI-ST575:165:C1P5BACXX:1:1101:13831:3779 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJJGGIJIJJHHFFDDDDDDDDDDDDBDBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14731:3871 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIIGIJIJIJIJIIJJHHFFDD@BDDDDDDDDDDDDDDDDDDDDDDECDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14772:3935 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +GAEGGIFHIDHECEFHIEGHHFFCB?DDBDDDD8BBBBD?CCDDDDDBDEDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:15067:3763 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIJGIIIJIGIJIJIIIFIHHFFDDBBDBDDDDDD@@BDCCDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15156:3801 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJIJIJJJJJJIJJGIIIHHFFDDDBDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDDCCCDED4(+,++4 +@HWI-ST575:165:C1P5BACXX:1:1101:16559:3892 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +CEF):6BD>B<D@DFCGBHGAHD<A;38,8=28:@@@B?CCDBDDBB?ACCD +@HWI-ST575:165:C1P5BACXX:1:1101:17255:3956 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGIJJJJJGIIGIIIJJGHFECDBDDBDD=BDDDDDBDDCDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17500:3790 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGJJJGIJJJJIJJGHIEEEFDDDDDBDD?BDDD7<BDCCD@DDBD?DDCDDCCD +@HWI-ST575:165:C1P5BACXX:1:1101:18440:3926 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIJIJJJJJJJJJIJJIJJHGFFDDDDDDDD?BDDDBBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19111:3987 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJGBHIJJIGCGIJAFHIGHFECDDDD@DDDDDDDB5??CCBDDDDDDECDDCDDDDCCCEEE:+4+++4++,:8 +@HWI-ST575:165:C1P5BACXX:1:1101:19557:3888 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIJIGGIIIGGIIIJIJJJHHFFDB?BDBDDBDDDB8BDDDDDDDDDBCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19920:3801 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJHJJJJIIJJIIIJIJJHHFFDDBDDDDDDDDDD@BBDDDDDDDDDDEDDDCDDDDDCCDDC:+++4+:44:>? +@HWI-ST575:165:C1P5BACXX:1:1101:1580:4241 +CCGATTTGAATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJHFGHEHIJJGJIGJJJJHHFFDB?BBDDDB@BBBDDD3:ABBDDDD<CDDCCCDDBCCCDDD:++44+:44:C@ +@HWI-ST575:165:C1P5BACXX:1:1101:2986:4163 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJGJJJJIJJJIIGHFFBB??@BDBDDDDBDDD>CDDBDDDDDEDDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4437:4204 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTCCACTCTCAC ++ +IIJHIGGIIJJJJIIJGIJHGFFDDDDDDDDBDDDDDBDDCCDBBB@DBDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5319:4088 +CCGATTTGGATCCGCCGAAGGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GIJJIJEHFIJIIEGHIJIHHEFFEDDDDDDBBB595?CC>6@BBBBDACCCCCDD +@HWI-ST575:165:C1P5BACXX:1:1101:5702:4094 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHIJJJJJJJJIJJIJJHHFFDDDDDDDD;BDDBBBDCDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5824:4111 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJIIIJIJJIJIJIJIIIJHHFFDDDDD@DDD@DDDACDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6984:4013 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GHBEEDFHGGGDF=EEHGHE4@=>?9;8??@B8BDBDBBDDCDDDDB?CDCDCCDDC +@HWI-ST575:165:C1P5BACXX:1:1101:7890:4076 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +FGBHGGGEHGIEIIGIGD@8BECDBD?@BD;=@DDB@?ADDDDDD@?CDDDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8052:4159 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +D??GAC@BDB?<BFDGI><==??CBBD?88;805575:?>>CDDBB;??AAC +@HWI-ST575:165:C1P5BACXX:1:1101:8080:4229 +CCGATTTGGATCCGCCGAAA ++ +HDFHEG=BF@DHEHGIJBHH +@HWI-ST575:165:C1P5BACXX:1:1101:8526:4228 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGCGGTTTACCGCGTTCCACTCTCAC ++ +JIJJJJIJJIIJJIJJJBGGGHHEDDDDDDDDDDDDDDDDDDDDDDDDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8765:4049 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJGIJJJJIJJIIJIIJGHHFFDB@BBBDD;@BDBDDDCCDDDDDDBCDDDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9822:4042 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJJJIJJJJJGHFFDDDDDDDDDDDDDDBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10184:4066 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIJJJJJJJJJJJJJJHHFFDBBDDDDDBDDDBDDDDDDDDDDDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11794:4113 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGIJJJJJJJJIJJJJIJHHFFDDDDDDDDDDDDDDDCCCBBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12356:4148 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIIHIIIJICGIIJIGIIIEBFFDB>BB@DD@BBD@BDBCDDDDDDDBDECDCDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:14554:4015 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCA ++ +IJJIGIDHIJIIJIHEIJJHDFFDDDDDBDDBDDDBB<@CCCDDDDDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:14736:4035 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +IJI<DDHHIGIIGCACGGG<ABFCBBBB8@B/5<B@<8BCACBBBBB8<C@@CCCD +@HWI-ST575:165:C1P5BACXX:1:1101:15267:4200 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJGGHIGIIJIIIGIJIJIHHFFDDBBDDDDDDDDDDBDDCDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17040:4246 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIJIJJJJIJIJIJJJJIHHFFDDDDD@BD;BDDD<@BDDDDBDDDDDCDDDDDCC +@HWI-ST575:165:C1P5BACXX:1:1101:17659:4106 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +III0DHGGGGGGHGGHBGIGHFDBBBBDDDD=@8DB@8@CCDDBDDD?CDCDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17880:4233 +CCGATTTGGATCCGCCGAGGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJEIJIIJJIIJII'=<BDDDBDDBDD-<BB<7@DDACDDDDDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19348:4134 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJGIGHGGIIIGAGHBHJHHBBCDBBDDDDDDDDB<<BDDCD@DBDDCDDDDDCCD +@HWI-ST575:165:C1P5BACXX:1:1101:1929:4262 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJJHIJJIJJJJJIJJIHHHHFFDDBDDDDDDDDDDDBD>CCDDDDDDDEDDDDDDDBCDCDDD4++44+:+4+>? +@HWI-ST575:165:C1P5BACXX:1:1101:3262:4389 +CCGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +FEB@FEFDGIICEFGBF@BB<?<A@@DDBDD=8-505?>@3070)529>@CCCCDC +@HWI-ST575:165:C1P5BACXX:1:1101:3996:4500 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCGT ++ +JJJIIIJJIJJIJIJJGIGEHHFFCDDDDDDDBDDD:BDCDDDDDD?CDDDDDDDDDCDCEEE:+,,,+4444:29 +@HWI-ST575:165:C1P5BACXX:1:1101:4255:4286 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAA ++ +?D<00)0????B3BDBF;E<AD6>@8888,3'005<B@??C@57;B-8?A:A?CDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4583:4479 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +FHIJJJGIIGGIJIJIGIJHHDF>BDBDDDD?BDDB<:BDDDDBDDDDDEDCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5886:4450 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +IJBHGGIIGGGGIGEHGGGBHDB?>;;;<BB69BB8@BBCDD@;5<BBDD@:3:CD +@HWI-ST575:165:C1P5BACXX:1:1101:6699:4365 +CCGTCTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIJJBGIIEGGHGIIBBE<EECABDDDBBB?BBB00<>@CDDD7;2<CDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7054:4268 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJGIIJJJIJIJJJIJIJEGFFDDDDDDDDDBDDBDDDDCDDDDDDDDECDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7021:4304 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGCGTTTACCGCGTTCCACTCTCAC ++ +IIJJEHIIGIJJJJJJJJIHHFFDB@DBDDDDDD@7@DDBACDBDDDBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7330:4272 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTC ++ +E??@GH?DF@@DFCHCBGE4<DDDD8@85,385<@BBD?ACD@B@BBB9(4@9 +@HWI-ST575:165:C1P5BACXX:1:1101:7985:4412 +CCGCGTTCCACTCTCACATTTCTAGTGAGACTTCGTCACTGTGGCACTTTCAAG ++ +IJIJIIJJJJJIGHHIJBHIJJJJGHHGGHHGFFFDCCDDCDDCDDCCDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9154:4399 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCCCGTTCCACTCTCA ++ +DB@@DG=F<FF38=FFBGG=6B<A?>/28?8'07@55<<C3@C87<ACC>4:<@:A +@HWI-ST575:165:C1P5BACXX:1:1101:9397:4390 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIIIIIGIJIIIJIJJGIJHHFFDDBDDDDDBBBBBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9626:4444 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIIJJJJJJJJJIJJJJJHHFFDDDDDDDDDDDDDDDDDCDDDDDDDDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:9920:4375 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJIJJJJJIJJJJJIJJHHFFDDDDDDDD@DDDBDDDDCDDDDDDDDEDDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:9928:4484 +CCGATTTGGATCCGCCGAAGCGGATCGTTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GI:@:BFHIEGHIIIGIFIBEDB<@BDCBDDBCBBBBBBCCDDDDDD@CDDDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:10347:4290 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJJJJJJJIJHHFFDDDDDDDDDDDDBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10607:4474 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTATCAC ++ +JIIGGIJJJJJJJIJJIJIHHFFDDBDBDDDBDDDDDBDDDCDDDDBDDEDDCCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11109:4422 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGCGGGGTTTACCGCGTTCCACTCTCAC ++ +CB@6?88D>*9??FEDG@E=<BBA@;>;<@;&)07@B5?9A:5<BD@8CD>C<:ACD +@HWI-ST575:165:C1P5BACXX:1:1101:11719:4366 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJJJJJJIIIHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11803:4357 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIGIIIIJIJJJDIJHIJHHFFDDDDDDDDBDDBDDDDCCDD@DDBBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12622:4263 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +FGIIJJIIBGIHGIJIEAE6<BCD8>@BBDBBD5550??A@BBBDBBC3:>9CCDD +@HWI-ST575:165:C1P5BACXX:1:1101:13888:4376 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJGIIIHI>GGEHGIHBGEBEFDDBDB@DD@B8B@<BBACDDDDDDBDEDDDDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:14985:4327 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJ:GGHJJJJJJIIJJJJIEHFFDDBDDBDDDBBDDDDDCCDDDDD@BDEDDDDDDDDCAACDD4+++++4 +@HWI-ST575:165:C1P5BACXX:1:1101:15294:4416 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +GE<:?=BGEC4?3BA;AGH>'=<ABBB@8?B;57<@@@89A@@BBD7<CDCC +@HWI-ST575:165:C1P5BACXX:1:1101:16020:4286 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAG ++ +JJJIJJIJJIJJIIIIIIJGHFFDDDDDDDDDDDDDBBDDDDDDDDDBDEDDDDDDDDDCCEEE4(,+++ +@HWI-ST575:165:C1P5BACXX:1:1101:16371:4398 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJIIHIJJJJIIJIFHFFDBBDBDDD=BDDDDDDDDDDDDD@@DEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17919:4354 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HHGIDDECHGGGFHHIIGGE<BEDD@D=?@B8:@877@@C:CBDDDDB?@CCCCCDC +@HWI-ST575:165:C1P5BACXX:1:1101:19347:4445 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCTCGTTCCACTCTCAC ++ +JJJIJJJJJJJJIIJJIJJHHFFCDDDDBDDDDDDDBDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19620:4333 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJGIIJIIIIJIIJJGIJHHFFDDDDDDDDDDDDDDDDDDDDDDDDDCEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20453:4255 +GATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HIIJJJJFGIJIIJIIGGIHHFDDDDDDDDDDDDB<BCCDDDDDDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20959:4324 +CCGATTTGGATCGGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GII<DCHEIIEI(;@;ABHE'9>BB'9?8;BBBBDB)5<A33:37<7<9ACAC(:CA +@HWI-ST575:165:C1P5BACXX:1:1101:20762:4415 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +EH88?BDFBB3DGGCEGIJ@EBD>ABD59=;;@BB@5<9:(5<@;B8<A@@<C>CC +@HWI-ST575:165:C1P5BACXX:1:1101:21156:4355 +CCGATTGGGATCGGCCGAAGCGGATCGGGCGAGGGGTTTACGGCGTTCCACTC ++ +?1?8@F(?8?);-4'---599><3''38'55077<@@B@?C(57755?(+4>9 +@HWI-ST575:165:C1P5BACXX:1:1101:21005:4492 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GFFGIICIIIHIEHGIDHEEEFDA@DDDDD=@DDD57?CC:97BDB?CDCDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:2208:4721 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJGHIJGIJJJJJJJIIJHHFFDDBDDDDDBDDBBDDD>ACB@BDDDDEDDDDDDDDDCCDDD:++(,+:34+>8 +@HWI-ST575:165:C1P5BACXX:1:1101:3594:4541 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IGHHIJIJJJJFBHHIEIEHHFDDDDDDDD=@DDDD9BDDDDDDBBBDEDDDCDDC +@HWI-ST575:165:C1P5BACXX:1:1101:4453:4680 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIIIIJIIJJJJIJJIJDHHFDDDDDDDDDBDDBBBBDDDCDDDDDDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4574:4514 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACC ++ +JJJIIJIJJJJIJGHJIJIHHFFDDDDDDDD;BBDBBDDDDDDDDDDDDEDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4512:4554 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IIJIJJFIJJJJJIJIIIGHHFFDDDDDDDD?DDDDDBDCDCBBDDDDDECDDDDDDDDDCDDE:44+,+:4+4>? +@HWI-ST575:165:C1P5BACXX:1:1101:6091:4740 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +CFF0:BDGG38?BBCBF<E=8B<<>8328;=;@B@9@<??CABDBDB@CDDD?CCCC +@HWI-ST575:165:C1P5BACXX:1:1101:7755:4724 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +BG06?GG><9?*).<F/545A99<;;;BD=;8<BDB5589CCB-0552AACC +@HWI-ST575:165:C1P5BACXX:1:1101:10497:4685 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIJJJJJJJJJJJIJJHHFFDDDDDDDDBDDDDDDDDDDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13319:4694 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCC ++ +??@?:DGH@*???2;CHBG<8BBA>3,8,823)-77B5??>ABDDD;(9 +@HWI-ST575:165:C1P5BACXX:1:1101:14064:4581 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAG ++ +IJJIJJIJJIJJJGIJIGGFHFFDDBDDDDDDDDDBDBBCDDDDDDDDCDDDDDDDDDCCCEED:+++4+ +@HWI-ST575:165:C1P5BACXX:1:1101:14735:4724 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIGJIJJIJFIGIIJJGEHFFDDDDD1;B;@BDB@<BACDDDDBDDDEDDDCDDC +@HWI-ST575:165:C1P5BACXX:1:1101:15633:4606 +CCGTCTGGTGTTCAGATCGGAAGAGCGTCGTGT ++ +JJF@GIIJFHHHGIIIIJJIEHIJJGHHHFCDD +@HWI-ST575:165:C1P5BACXX:1:1101:16660:4702 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIGFGHJJJJJJJJIBGIIFEFFDBB???BD/5@B<:@BD>CBDDDDDDEDCDCCDD +@HWI-ST575:165:C1P5BACXX:1:1101:18593:4710 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJIIHEHCHIJJIJIGGGEEFFDDBDB?BB/7<BB@D@CDCBBDDDDCCDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19241:4545 +CCCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJIJIJJJHBHHGIEGIHEFDDCDDDDBDDDDDBD77?CCDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19601:4684 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +EGEEGEHIJGIGHIIGGEEBEEDCB@DD@BDDDBBB@BBDDDDDDDDDDDDCDCCDC +@HWI-ST575:165:C1P5BACXX:1:1101:20709:4735 +CCGTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIJIJJJJIBHGDBBAEFDCDDDDDDBBDDBB:BCCDB@B<BDC@CDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:21224:4560 +CCGTTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +BGI6HDCGHGCF;BG;@BHEEDFCDBBD5;BBDDDB55?C>A8@<BDD?DDCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:2354:4948 +CCCATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJIGIGHGHIJJJJJJEHGIHFFDCDDDDDDDDDDDBD?CDDD;BDDDEDDDDDCC +@HWI-ST575:165:C1P5BACXX:1:1101:2921:4941 +CGTTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIIJGJIIEEHIGIIIJIEEHGFFDCDBDB=BBDDB<@CDCD@B@<BDEDDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4132:4919 +CCGATTTGGATCCGCCGAAGCGGATCAGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJGHJIJJIIIJJJJJJJHHFFDDDDDEDDDBDDBBBBDDDDDDDDDDEDDCDDDDDDCCDCD:44(,+:444:? +@HWI-ST575:165:C1P5BACXX:1:1101:5510:4838 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IGGCFHG9C<BBFHEHHGB8FABBBD=<?BD@DB;5<@BCCAB<@@BBDCC@CCDCC +@HWI-ST575:165:C1P5BACXX:1:1101:6248:4986 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIJJJJJJJJJIJJJJJHHFFDDDDDDDDDDDD@BBDDDDDBDDDDDEDDDDDDDDDCCDEE:+++4(444:@? +@HWI-ST575:165:C1P5BACXX:1:1101:6936:4836 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHGHJIIHEIIIIIIGJHHFFDDDDBBDDBDDDBDBBCDCB<7BDDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7709:4867 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +IIGHIIGJJJJJIJJJJJIEHFFDDBBBDDDDDBDDDBBDDCBDDDDDDEDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9439:4978 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJJJJJJJJIHHFFDDDDDDDD@DDDDDBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9572:4907 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJIJJIIIJJIJIHHFFCDBDDDDD8:<BB5<BCDDDDDDBBCDDACDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:9900:4892 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIIIGIIIIJJIJJIJIJIHHFFDDBBDDBDDDDDBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9950:4989 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIJGIJJJJJJJJIJJIJJHHFFDDDDDDDDBDDDDDBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10290:4901 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GHEHIJIIJGGFFEHIAHGHHFFDDBBDBB@275@<7<BCCDDDDDDDDECDCDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:10794:4755 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIJIJJJJJJIJJJJJGGFFDDDDDBBD@BDBBBBBDDDDDDD@BCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11203:4970 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJEIJJJJJJJJHHFFDDDDDDDDDDDDDDDDDDDDBDDDBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11597:4816 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJIGIHIJIFIIEGHFHFFDDDDDDDDDDDDD:BBCCDDBDDDBDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11814:4971 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJIJJJIJJIIJJJJIHHFFDDDDDDDDDDDDDBBDDDDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12815:4804 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GIJHIJJGIEEGIDIIAEIHHFBBBBBDDDDBDDBDBBBDCDD;BDDBDCDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15064:4795 +CCGATTTGGATCCGCCGAAGCGGATCGGTCAAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJJJIIJIJJIIIHIJIIFHFFDDDDDDDDDDDDDDBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15338:4874 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJBDECHIHGIJJJCHIJGHFFDDDDD@DDBBDD@BBBCCDDDDDDDDEDCCDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:15739:4984 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +CDADDD?G>???FFEDHEG<<DBAB?@=5;22)07:@5<9AC8BDDD<C>>C9>:@C +@HWI-ST575:165:C1P5BACXX:1:1101:16043:4995 +CCGTTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCCTCTCAC ++ +IJJHIJJJJJJJJIJIIJJGGFFDDDDDDDDDDDDB<BDDDDDDDDDDCDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17608:4859 +CCGATTTGGATCAGCCGAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HGG)?DDGIHGG@H<BGAB>EFF>BDB?BB?8;@B578>>CDDB<;8<3:><CCCD +@HWI-ST575:165:C1P5BACXX:1:1101:17879:4904 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIJJJJIJJJJJJJJJIGHFFDDDDDDD=BDDDBDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18072:4949 +CCGATTTGGATCCACCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJIJJJ>GIIIJJJHHFFDDDDDD?BDDDDDDDDDDBBDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18453:4916 +CCGATTTGGATCCGCAGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +EGG:FEGHHHGI;FH<FGHIIJIGHEFD=AD;@DBBBBBDDDDBBDD@DEDCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19254:4780 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +EGEFEDFGGGGHGIGBFHIBEFDDBB??<?@;B8B<75?ADC<DDDB?CDCC<CCDC +@HWI-ST575:165:C1P5BACXX:1:1101:19861:4798 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIJJJJIIIIJJJJJIIGHFFDDDDDDDDDDDDDDDDDDDDDDDDDCEDDDDDDCDDDDEEE:44:44:++:>? +@HWI-ST575:165:C1P5BACXX:1:1101:19870:4937 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJHGIJJJIIGHIEHIIIGHFFCDBDD@BDBBDDD7@BCDCBDDDDD<CDDDDDDDDCDDEDD:+4444:++:>8 +@HWI-ST575:165:C1P5BACXX:1:1101:19796:4989 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJJJJJJJJIJJIJJJJIHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDDCCDEEE:+,+4+:4+:>8 +@HWI-ST575:165:C1P5BACXX:1:1101:20053:4972 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACC ++ +IIDDHGIJHGGIGHIIDIHHFF>ABDDDDD?DDDDB<BCDDDDDDB@CEDDDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20782:4908 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GIIAFAHIJE<DGII=BHIBHEFCBDDD?@B=BBDD5@@CCDBBDDDDCDDDD@ACC +@HWI-ST575:165:C1P5BACXX:1:1101:1189:5021 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTATAGTGAGACTTCG ++ +GDG@DDCE>BFGGIH?B@ABGE<B>3=>?@@BBBDBD8?33>:&57@8ADDC<@@CC?AADD(::4>:44:+:4:? +@HWI-ST575:165:C1P5BACXX:1:1101:1686:5115 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJJJJJHIGHHIJJJGJIHHFF@B@BBDDDBDDDBBDD9@CBDDDDDCDCCDDDACB@ACEDD:+444,:>>>4< +@HWI-ST575:165:C1P5BACXX:1:1101:2938:5127 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HIEDFHIG<BGHIEGIGGIHADECDDBDBB@BD<BBBBB9ACBDDDDDCDCCCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:3405:5036 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCCGTTCCACTCTCAC ++ +IJJJJJJIIJJJJJJIJHIHHFFDDBDDBDDDDDDDDBDCDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7045:5134 +CCGTTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCAACT ++ +F8@:??006/B<?;(7@BGA:'993''9588'5:@<05?AACD5<B;B((4: +@HWI-ST575:165:C1P5BACXX:1:1101:7123:5216 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJIIJIJJGIEGIGIGHHFFDD?BBBDD@BDDBBBBCCCBBDDDDDDDDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:8358:5145 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIJJIIIIJJJIJJJIIHHFFDBBDDDDDDDDDDDDDDDDB@BDDDDEDDDDDDDDDDDEEE:+,44+:4+4>< +@HWI-ST575:165:C1P5BACXX:1:1101:8840:5173 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGIJJJJGIJJJJJJIJFBEFDDBDDDDD=BDDBBDDDDDDDDDCDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9731:5160 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJDFHJGIIIIJJIJJIJJGHFFDDDDDBDD;BDDDDDDCDCDDDDDDDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:10830:5146 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IGHIIJIIJJIFEEIJBHABHFDDD??DDDBD@@BD:BDCCDDDBB?CDCCCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11032:5068 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJIJJIIJJIJJIJJJGGFFDDDDDDDD;BBDBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11565:5085 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIEHHIJJHIJJJJJJIIFFFFDDBDDBDD?BBD<<@BDDDDDDDD?CEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12209:5017 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTC ++ +?D)00B0BDF>*?CGB@5AA<9638'9=8,2257855:@C:>@50552<3>:9 +@HWI-ST575:165:C1P5BACXX:1:1101:12070:5238 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHIJJJJJJJJJJJJJIHGFFDDDDD?BDDDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12614:5234 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHIJJJJJJIJIJJJJJHGFFDDBDDDDDDDDDDDBBDDCDBDDDDDEDDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:12934:5066 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIJIJJJJJJJJJJJJJGEFFFDDDDDBDDDDDDBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12889:5153 +CCGTCTTGGATCCGCCGGAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +JJGHIJJGIIJJJGHIGIHFFFDDDDDDBBD1BDD@57BCDCD@DDDBDECDDCDC +@HWI-ST575:165:C1P5BACXX:1:1101:13006:5008 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +:C????;F98??B=1;AEG4-=5=>/,'(,,,))57@5??CCDDDD@2?C@C +@HWI-ST575:165:C1P5BACXX:1:1101:13701:5054 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIJJJJJJIIJJJJJJJGHHFFDDDDDDDDDDDDDDDDDCDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13818:5190 +CGATTTGGATCCGCCAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJHIJJJJJJJJGIJJJHIJJJJJHHHFDDDDDD=8?CDCDDDBDDCCDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14721:5211 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACC ++ +DHBHIF@GHIGEGGIBHG6EHEDFDDDDB@;?BDDB<?CCCDBB<B(3>CCC:@@CD +@HWI-ST575:165:C1P5BACXX:1:1101:15180:5074 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +)CGHIJIJJIJJJIIIGGHHHFFDDDDDDDDDDDDDDDDCDDDDDDDDDEDDDDDDDDDDCEEE4+++++::4>C@ +@HWI-ST575:165:C1P5BACXX:1:1101:15733:5028 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJHIJJJJJJJJIJJJJJHGFFDDDDDDDDDDDDDBDDDDDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:16783:5148 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIIHJJIJJJIJIIJJIJIGHFFDDDDDDDD?BDDB@BBDDDBB@DBDDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:17093:5065 +CCTATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJJJJJJJJIGIHHFFDDDDDD@DDDDBBDDDDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18177:5052 +CCGCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJJIIGIFGGJIGHA/@BEBD?>BDDDDDDDDDD05?CDDDBD;7<ACDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18454:5064 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIGGIJJIGIIICBCEGGDBEFEDBDBDB?B8@DDD<BBDDCBDDDBDCDDDDCCCC +@HWI-ST575:165:C1P5BACXX:1:1101:19514:5155 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +@GHAHGHIIICH>GIGHBBBHEBABBBD@BDDDDDBBBBDDDDDDDDDDEDDDDDCC +@HWI-ST575:165:C1P5BACXX:1:1101:21070:5172 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +?F<086BFHE<B)BCBF8AAA=9/>8?B,8=BDDDB758>CA<DDBBBDDCDCDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:1152:5446 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTT ++ +JHJFHGIIIHEDEEGIHBAE:BFDB>BD??@8<BDB05?3(:<7<5-<CDCC?ACCCBDD4:>:4+4444>:4+ +@HWI-ST575:165:C1P5BACXX:1:1101:2332:5476 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIGIJJJJIJJIJJJJEHHHFFDDDDBDDDDDDDBBBDACCDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:3549:5422 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJIJJJIIJJJJIJJGIJHHFFDDBDDDDDDDDDBDDDCDCDDBDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:3934:5255 +CCGATCTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJJGG6DHIJJJIIJJIJFHFFDDDDDDDDD@BDDDDBDDDDDDDDDDDEDDDDDDDDDCCEED:44+,+:++4>? +@HWI-ST575:165:C1P5BACXX:1:1101:3779:5257 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJIIJJJIJJIIJJJJIJIHHFFDDDDDDDDBDDD@BBDCCCDBDDDDDDDDDDDDDDDDDDDE::44,+:444@? +@HWI-ST575:165:C1P5BACXX:1:1101:3981:5396 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +CGD@FAFIIIEGEGEBFGGBGIDFEDDC=>?>BBD<95?ACCDDDDBBDECDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4352:5435 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJIJJJJJJJJIIJHGFFDDDDDDDDDDDDDDBDDDDDDD;@DDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5757:5381 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +CFG>BGBFGGHD@BFFHGGEEED<??;?@BD'5<@BBB89CCDDDDB?CDDCCCDDC +@HWI-ST575:165:C1P5BACXX:1:1101:7084:5290 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJIIJJJIIJJJJJIJIIHEFFDDDDDDDDDDDDBBDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7550:5435 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +IIJIJGIJIJJJIIJJJFIGHFFCDDDBBBDBDBD<<BBCDDDBBBDDADDDCCDC +@HWI-ST575:165:C1P5BACXX:1:1101:8518:5454 +CCGATTTGGATCCGCCGAAGCGGAT ++ +HIGGGCHIIHIEGIGEG<EAHFDCB +@HWI-ST575:165:C1P5BACXX:1:1101:8740:5499 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIFHJIJJIIJJIJJJJJHHFFDDDDDDDD?BBDBBDDDDCDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8810:5337 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJGGIIIJJIIJEEHIGBHHEFFDDBDDBBD=BBB@BBBDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10317:5279 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGIJJJJJJJJJJJJJJHHFFDDDBDDDD?BDDDDDDDDCDDDDDDDEDDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11346:5362 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCACTCTCAC ++ +JJIIIIIHIJJJJJJJJJJHHFFDBBDDDDDDDDD@BBDDDA@BDDB@DDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12293:5318 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IGJEFHIDGGGGGHIJFGIHHFFDDBBDBBDDDB8@9<BDCCBBDDBDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12385:5426 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJJJIJJJJIGGFFDBDDDBBDDDDDBDDDDDDDDDDDDDEDDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12559:5359 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +BGA0@FGBGDF<?=CF<@E=A<<<;83;88?3:555:8<9:@@7@DDB<@(:<AC@ +@HWI-ST575:165:C1P5BACXX:1:1101:13619:5383 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIGFGIIGIFHIJJJJEGIEFFEDDDDBBBD?BDD@@BDDCCDBDDDDDECDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14813:5367 +CCGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJHIIGIHIIIJJJJGIGEHFDDDDDDDDDBDDDD:@CCDDDDDB?ADDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:16958:5440 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJ<HIJJJJJJIJJJJJJHHFEDDBDDDDD;BDDDBDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17064:5269 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJDHIJJIIJJJJIJJJJHHFFDDBDDDDD:BDDBDDDDDDDDDDCDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18711:5374 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJJJIJJJJIJIIJJIIJHHFFDDDDDDDDBDDDBBDDDDCDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19795:5388 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +)?@)86;FCF<</BD6A4@:'=?>?8;?528@95B7<529ACBDDDD?CD:A<@@CC +@HWI-ST575:165:C1P5BACXX:1:1101:1536:5742 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HJIEIJIGGBHGICIIBHIGHFFDBBDD?BDB9BDB5<?9ACD<;7;?CACCACDCD +@HWI-ST575:165:C1P5BACXX:1:1101:3186:5524 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHGIJHIIIJJIJIGEHBHFFDDBBBDBBDDDDDB9B9@CDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:3099:5544 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIJIJGHIJJJIJJJIGEHFFBBB@DDDDDDDDDDDD>CCDDDDDDDEDDDDDDDDC@CDEE:++4,+44+(:2 +@HWI-ST575:165:C1P5BACXX:1:1101:4347:5721 +CAGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIHGIGAAEHIIIEDHEHJIJHFFFDDDDDD:@DBB77BCCCBDDDDDCDCDCCDDC +@HWI-ST575:165:C1P5BACXX:1:1101:5268:5661 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +CF:)8)8DDB<??CFBHAG=AD<8>;;88;8'00:7@889AA5@BDD@DECC?CDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6568:5658 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIGIJJJJJJJIGJJGGGHGFFDDDDDDDDDDBDBBBDDDCDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7344:5519 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIGHIJJJIJJJJIIJGGHFHFFDDDDDDDDDDDDBBBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8216:5553 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJEIJJJJJIJJIJJJGGEHFFDDDDDDDDDDDDDDDDDDDDBDDDDCEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8183:5554 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTC ++ +?DFHI;?B<GGHG2-7;5@E8?BA@?BB<?BB@-&007<9CDB7<5;?3:@:9 +@HWI-ST575:165:C1P5BACXX:1:1101:8832:5696 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJIJJJJJIIJJJJJHHFFDD@BDDDDBBDDDDDDDDDBDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9211:5626 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGIJIJJJJIJJJJJJJHHFFDDDDDDDDBDDDDDDDDDDDDDDDDDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:9028:5675 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJIJJJIJJIJIHHFFDDBDDDDD@BDDBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9789:5670 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGGIJJIIIIJJJJJJJGHFFDBDBDDDD;@DDBDDDDDDDDDDDDDEDDDCDCD +@HWI-ST575:165:C1P5BACXX:1:1101:10507:5654 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +FA??68BGBB@BDGC;FEH:8BBBD@D@?@B275@:@@??CC<BDDD<CD>C<:ACD +@HWI-ST575:165:C1P5BACXX:1:1101:12148:5538 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJEIJJIJJJJJJJJJIIFGFFDDDDDDDDDBBDDD<BDDDDBDDDBCADACACCD +@HWI-ST575:165:C1P5BACXX:1:1101:12149:5686 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGIJJIJIIIJIJJIJIGHFFDDDDDDDDDDDDDB@DDDDDDBDBBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12546:5747 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HGGHEGDBFBBDDGGGIHGEEBED@@D=??=;57975<@?>@BDBBBDCDCDDCCDC +@HWI-ST575:165:C1P5BACXX:1:1101:13615:5616 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIJJJIGJIJJIJJJIHHFFDDBBBDDDDDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13537:5735 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJIJIIIJJJIGIIGGHFDDBDDDDDDDDDBBBDCDDDDDDDDDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:14584:5542 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJIJJJJJJJJJJJJJJJHHFFDDDDBDDBDDDDDDDDDDDDDDDDDCDDDDDDDDDDCCDEE4(,+++4 +@HWI-ST575:165:C1P5BACXX:1:1101:15724:5746 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACC ++ +IJJIJJJIJJJJJJJJJJJHHFFDDDDDDDDDDDDDB@DDCDDDDDDDDEDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17227:5625 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTC ++ +FGIB?B*?3?BBFBFG6A8;:EB<9A?=;;;289@&0893@BD;5;(+:>C9>@ +@HWI-ST575:165:C1P5BACXX:1:1101:17881:5507 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJIJJJJJJJJJJJHHFFDDDDDDDD8@DDBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20112:5554 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +>GI:D;DHFG@B8BF;AEHEEDD>@>>B5=BDDDD@B@<>CCDDDDDBDECCCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20322:5548 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +BHI06??DGE<D?BB@FHGEHE>/;3>B?@B=BB8700<CD37BBDDD3>CC<(+: +@HWI-ST575:165:C1P5BACXX:1:1101:21233:5679 +CCGATTTGGAGCCGCCGAAGCGGATCGGGCGAGGGGTTTACCGCGTTCCA ++ +1?D)0)0?99)9(7@'5':<<>6'9'88'27BB8B970(+>37BDBD?CD +@HWI-ST575:165:C1P5BACXX:1:1101:1959:5920 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJHGJIJIJJJJFIJJGIFHFFDDDDDDDDBDBDDDDD?CCDDDD;@CDDDDCDDDDDCCDED:44(4+4,4+>8 +@HWI-ST575:165:C1P5BACXX:1:1101:1924:5978 +CCGATTTGGATCCGCCGAGGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJJIIIGJJIGHIJIGJGHGFDDDDBDDBD85:BDBDDBACDDDDDDDCDCDDDDDDDDCCEDE4+,44+4+++:8 +@HWI-ST575:165:C1P5BACXX:1:1101:4153:5778 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GIJGIGGIJJJJIJJIGIIHHFFDDDDDDDDDDDD@<<DDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4024:5788 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GIDHIJJJJJJJIJJIJJJHHFFDD?BDBDD:@BDBBBBCCDDDDDDDDEDDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4463:5945 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTC ++ +HHBGGHEAFDFDGIGGGEG<EBBB?;;/,8@87@9959@CDCDBB<;BCD>@C +@HWI-ST575:165:C1P5BACXX:1:1101:6188:5778 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHJJJJJJJJJJJJJJJHHFFDDDDDDDDDDDD@BBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6867:5946 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGTTCCACTCTCACCATTTCTAGTGAGACTTCGTC ++ +JJJIJJJJJIJJIIJJIGIHHFFDDDDDDDDDDDDDDBDDDCDDDDDEDDDDDDDDDDDEEE@44>44:+,4>?CB +@HWI-ST575:165:C1P5BACXX:1:1101:7535:5814 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIGIJIJJJJJJIJJJGGHGFFDDDDDDDDDDDDDB7@CDDDBDDBBCDCDDDCAC +@HWI-ST575:165:C1P5BACXX:1:1101:7794:5903 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIGIIGGIJJJJIIJHIJHHFFDD@DDDDBDDDDDDDDDCDDDDDDBDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9106:5832 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIIIJJJJJJJJIJIIEEFFDB@@BBDD;:<BBBBBDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9392:5986 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GGIGEEBHHDEHIJIJ8;A>BEDC@DDB?BB-95<05<9:CDD75<<A3>ACCCCC +@HWI-ST575:165:C1P5BACXX:1:1101:9549:5793 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +DG>@FG6BFAGGICGHGGIBEEDCD?>8@??;@@BBBBBCDDDDDDBBCDCDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10541:5867 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJFHJJJJIIJJJJJJJJHHFFDDDDDDDDDDDDBBBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11397:5818 +CGATTTGGATCCGCCGAAGCG ++ +H?)@DGI@<?<D8;F4';-45 +@HWI-ST575:165:C1P5BACXX:1:1101:13482:5805 +CCGGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJHIJJGIIIIJJIHIIHHFFD/;BDDBDD:DDDDBBBCCCDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13731:5797 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIIIJJIJIJJJIJJIIJHHFFDDDDDDDDDDDDDDDDDDDDDDDBDDDDCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13983:5888 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIEIIJJJIIJGGIIIIIHHFFDDDDDBDD?BDD<9BBCCCDDDD@DCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13915:5939 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGIJJJJHIJJJJJJJGHHFFDDDDDDDDBDDDDBDDDCCDBDDDDDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:14076:5802 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIIJJJJIJJIIIJIIIHHFFDDDDDDDDDDDDB7<BDCCDDDDDDDEDDDDDDDDDACADC4+,,44:+::C? +@HWI-ST575:165:C1P5BACXX:1:1101:14165:5883 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +GIJIJGAHFIJFIGGHGHHFECDDBBBDBDDBD<<:9<BCDDDBDBDDCCCDDDCCCBCCA>DD4+++++4 +@HWI-ST575:165:C1P5BACXX:1:1101:14716:5991 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JIJGIJJJJJJJIEHJIJIHHFFDDDDDDDDDDDDDB@DDDCDDDDDDDDDDDDDDDDDDDDDE:,++4+:44>A@ +@HWI-ST575:165:C1P5BACXX:1:1101:15153:5829 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGAGTTCCACTCTCACCATTTCTAGTGAGA ++ +DHIHIGGIJIJJGIJIJGGFHFFDDDBDDDDBDBDBD<BCDDDDDDDCCEDDDDCDDBA>CADD4+++++4 +@HWI-ST575:165:C1P5BACXX:1:1101:15618:5813 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +HGGGGHIJIEGBFFEGHDGHHFFDBBBB@==89@@<07@>@CBDDDBD?DCDDDDA +@HWI-ST575:165:C1P5BACXX:1:1101:16278:5962 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAAGGGTTTACCGCGTTCCACTCTCA ++ +GHIABFHDGGIG>FGIGFHFDFDBD?BDDDD8BBD@BABC:AB;BDDDCDDDDCDA +@HWI-ST575:165:C1P5BACXX:1:1101:17404:5768 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIFDGHIJJJJJJJJJIJIEHFFDD@BDDDD;@DDBBBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18508:5770 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIGIJJJJJJJJIJJIJIHHFFDDDDDDDDBDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18654:5893 +CCGATGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCGTC ++ +IJJJJJJIJJIIJJJF<BHFFDDDDDDDDDDDDDB:@CCDDDDDDDDDDDDDDDDDDDDEEE:44444:4::@?CD +@HWI-ST575:165:C1P5BACXX:1:1101:20005:5823 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +FIJ6DBHIIG>HCHIIGHIEHFFDDDDB@DBDDDDDDDBDDDDDDDDDCDDDDCCDDDCC@ADD:(++,+4++4:2 +@HWI-ST575:165:C1P5BACXX:1:1101:20461:5768 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +IJJDFFHGIHIIJIGGIJJHFEDCD>BD@BBB@DBBBBDDCDDDDD@?AA@CCCA> +@HWI-ST575:165:C1P5BACXX:1:1101:1693:6048 +CCGATTTGGATCCGCCGAAGCGGGTCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IGGGHGGGHEGHGHGIIDEGAHFBCDDD@@D9:B8::B?3:ABB<@DD?:CC<@@CCDCCCACC444:4+:,44>8 +@HWI-ST575:165:C1P5BACXX:1:1101:3291:6004 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIIJIIJJJJJIJJGHIHHFFDDDDDDDDDDDDDDBD?CDDDDDDDDEDDDDDDDDDCCEEE:4444(44++>8 +@HWI-ST575:165:C1P5BACXX:1:1101:3937:6198 +CCGATTTGGATCCGCCGGAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHIJJJIIIJJJJJIJHHFDDDDDD'8@DBBDDDDDDDDDDDDDDDDCDDDDDCC +@HWI-ST575:165:C1P5BACXX:1:1101:4273:6038 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIGIGIIIIIIJIGIIIGFHFFCDDDDDDB:@BDBBDDC>ABBDDBDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4445:6115 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +HGF<FHEGDEGGIGGGG<AEEBE<;99=?BDDDBDB5<B3:@B<@B7BCCC>C>CA +@HWI-ST575:165:C1P5BACXX:1:1101:4344:6153 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIJJJJJJJJIJJJGBHFGFFDDDDDDDD@DDDDDDDACDDDDDDDDEDDDDDDDDCCCDEE4+++,+4444@? +@HWI-ST575:165:C1P5BACXX:1:1101:5212:6077 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIJIIIDCGGHIJBHHIIGEH<B>@BBDDDD;B@DDBDBCCCDBDDBDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5854:6193 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACT ++ +JJIIJJJJJEHIJJJJJJJHHFFDB@BBDDD:@BDBDBBCDDDDDD;BDDCDDDDDDDDCCDEE>4444+4+4 +@HWI-ST575:165:C1P5BACXX:1:1101:6093:6246 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +JJIEIJGHIIIJFJJGGBGEHFB>B>B@BDD25<B@BBDDDDDDDDBDDEDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8105:6158 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +BGH>BCDHIFEHHIIEA6AAEE6<>@B@??@/7<B@DBDCCDBDDB7?CA@:9>CDD +@HWI-ST575:165:C1P5BACXX:1:1101:8253:6223 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGGTTACCGCGTTCCACTCTCAC ++ +DGFH=DD?3BFDCCDA;'@8<AB88AB=?BBB@B5&)58337B7&72?CCC3>@CD +@HWI-ST575:165:C1P5BACXX:1:1101:8552:6031 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +FE0@FGCGBGEHIIGJGABEHFCDDBDDDDDBDBD578ACAB<BBBBDECDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8575:6101 +CCAATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +BDHIGCBDHF>FFFEAG<FGB=EFBADB=??/880557??>CDDB5<<<4@CACCCD +@HWI-ST575:165:C1P5BACXX:1:1101:9569:6015 +CGGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJIJJJJJFIJJJJJJJHGHFDDDDDDD/=5:BBBBBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10905:6024 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +CCD@?@8D@*9?DHDBG<E<BEDAB>@?<?B679B@B5??CCBBDDD?CDCDDC>CC +@HWI-ST575:165:C1P5BACXX:1:1101:11477:6010 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +JIIGIJIJJIIIJJJIIIIEHFFDDBB@?BDBBDDDDBBDCDDBBBD?ADDD +@HWI-ST575:165:C1P5BACXX:1:1101:12389:6154 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +JJJIJJJJJJJJJJJJIGIEHFFDDDDDDDDBDDDBDDBDDDDDDDCDDEDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12820:6019 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GGHE>HGHGEGGIJE;FHI>6BDDD@BDDDDBB@DDB8?CCD57@@B@DD:A9A:@C +@HWI-ST575:165:C1P5BACXX:1:1101:13503:6047 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJHIJJIJJJIIIIJJGIGHGIHFFDDDDDDDDDDD:BDDCDBDDBBCEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14071:6041 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JGB@FHIHIEI@BFEDHGIHHFFDDDD=B?B@BBB<B@BC@CD@BBBD?ACD?CDCA +@HWI-ST575:165:C1P5BACXX:1:1101:14685:6102 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIJJJJJJJJJIJJJJJHHFFDDDDDDDDBDDDBBBDDDDDDDDDDDEDDDDDDDDDCCEEE:++,4+:::@CB +@HWI-ST575:165:C1P5BACXX:1:1101:14930:6229 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIGIIGIEGGEGHHGIJHHFFCC@DDDDB@BDD@7@BCCCDDDDDDDDCDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15280:6053 +CCGTCTTGGATCCGCCGAAGAGGAT ++ +JIIFGIIIIIJJIEIGBGIHEHHFF +@HWI-ST575:165:C1P5BACXX:1:1101:15344:6191 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJIJIJJJIIGJGGIGHFFDDDDDBDDBDDDDD@DDDDDDDDDDDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:16410:6086 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJIJJGIJJJJJJJJHFFFDDDDDDDD=BDDBDDDDDCDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:16374:6145 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIIJJJIJJIJJIIJIHHFFDDDDDDDDDDDDDDDDCCDDDDDDDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:16749:6158 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJIJJJJIIJJJIJJJIJHHFFDDDDDDDDDDDDDDDDACCDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17553:6192 +CGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIGHJHIICCGEGIIJ>GIGGFFDBDDDDDDBDDDB:BCCCDBDDB?ACDDCDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:17938:6081 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGJJJJJFIJIIJJIJJHHFFDDDDDDDD=BDDDDBDDDDDDDDDBDDCDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19315:6150 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +B8CF)?69B@B*9?BFGB8@AADFABDB?;@=?B<5)5?3:AB@75;BCDCDACCDC +@HWI-ST575:165:C1P5BACXX:1:1101:19532:6018 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIIIJJJJJIGIGHIIJEFGFFDDDDDDDDDDDDB5<BCDDDDDDDDCEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:21261:6248 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJI<FDGHFIBFGGIGIIJGEHEFDDDB@DDDDDDBB@?>CDBB@@D7CDCDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:1089:6262 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIBFHGIJIGHHIHIJGHB<BFCB;?B?@@8755755BCACB@BD<BCACCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:1439:6319 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +?+@1?:?D499*):8?D6A64B@'9,99=/938,;:@583:(5;BD<0<A>CA4(::<CA@CCA4++4:4>4(4:8 +@HWI-ST575:165:C1P5BACXX:1:1101:1829:6451 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGATTTACCGCGTTCCACTCTCAC ++ +JJJIJJIJJEIIJEHIEGIHHFFCDBDDBDDDBDDDACCDDDDDDDDDADDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:2484:6444 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +G?@DFC=FG*9?D9BFHGHA<BBCDBB=<@B87-:9B@?9AC8B@DDBDDAC<@CCC +@HWI-ST575:165:C1P5BACXX:1:1101:3350:6364 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IF6?@D?DGHDGGGGG;EB8BD>BBB83<@:=B-9@B<?DCCBDDDDDCCCCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:3699:6336 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +?@6?@C=DG<DBBFFFF4A8ADDBB;B8<BB/58B@B<<>ACBDDDB8?DCCA@ACC<@>@@DC:44+,++(,:>? +@HWI-ST575:165:C1P5BACXX:1:1101:3874:6280 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIJJJIJJJIJIIIIJIEHFFDDDDDDDDBDDDDDDDACDDDDDDDDDDDDDDCDBCCDDDE:+44,+:44:C@ +@HWI-ST575:165:C1P5BACXX:1:1101:4404:6415 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJIHIJJIIIJJJJJJIIIFHFFDDBDDDDDBDDDDDDDDDDDDDDDDDEDDDDDDDDDDDEED:++4,(44+4:< +@HWI-ST575:165:C1P5BACXX:1:1101:5416:6451 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +H@GD@?;FDBFHHIBHGG>EB?DCDDDDDDDDDDB<57@>:>@<@@@BCCDCCDCCDBC:::CC4+++,+:4+4>8 +@HWI-ST575:165:C1P5BACXX:1:1101:7066:6455 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJFJJIJJJJIJIJIHHFFDDDDDDDD?BDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7488:6251 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGJJJJJJJJJIJJIIIHHFFDDBDDDDDDDDDBBDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7486:6329 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIJJIJJIJJJIJJJGHHFFFDDDDDDBDBDDDDB7?CCDDDDBB??ACCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7643:6450 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGT ++ +JEDFHJIHICIGIGIJGJJHHFDAB>?=??==@BBBB<BDC>@DBB +@HWI-ST575:165:C1P5BACXX:1:1101:8454:6487 +CCGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIGHJIJICHGHHIJJGBGFFFDBBDDDDB?BDBD55?C>CBDD@B8<>ACCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9137:6329 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIFIJIJJIJJJIJJJIIHFFFDBBDBDDDBDDDDDBDDCDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9587:6277 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTATCGCGTTCCACTCTCAC ++ +IIBGEIIJJIJJJIJJJGHEHFFDD@DDDDDBDDD<7<?A(:ADDBD@DEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9536:6348 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +GI<@DHHIICHIJJIJJII<BFFDDDDBBDD69@D@BBBCDDDDDDD?CCCCDDCDDBC>ADDD4(++,+444:@( +@HWI-ST575:165:C1P5BACXX:1:1101:10054:6289 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGTTCCACTCTCAC ++ +JJJGIJJIJJIJIIJJGHGHHFFDDBDBBBDDDDDDB@BCCCDBBCCCCCDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:10173:6294 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGGGCCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCGTC ++ +JJIGEIJIJJJJJJJJIJJHHFFDDDDDDDDBDDDDDDDDDDDDDDDDCACDDDDDDDDEEE@::4,+4++4:?C@ +@HWI-ST575:165:C1P5BACXX:1:1101:10764:6333 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +HIJIJIGICGGIIEHIABEGHHDEC?>>;?B8=85757@ACDDBDDBDCCC@ +@HWI-ST575:165:C1P5BACXX:1:1101:11047:6477 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +GDGDGEGGFFBB?CDEB;EEEEF<>;?@5;?8<58<:@B>C>@;BBDBACCC +@HWI-ST575:165:C1P5BACXX:1:1101:11411:6372 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTC ++ +JIJIJIDIHIJJJFGHBGGHHFFDDBB=?BB?BDB@8@BCDDDDDDDDCDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:11665:6448 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJIIJJJIIJJIJJGIIHHFFCDBDDDDDDDDDBBDDDCDDDDDDB?DDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12774:6457 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GIBDHIGGIJIIJEIF@@EAEFBBDB@?@@?;8BDBDB?CC>9B7@@<CDDCDDCAD +@HWI-ST575:165:C1P5BACXX:1:1101:13106:6369 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJJJJJJHJJHHFFDBBDDDDDDDDDBDDDDDDDDDDDDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:16704:6321 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIIJIJIJIJIFEIIIGIEHFEDDDD@DDDDDDD@<BDDDDDDDDDBDEDDDCCDD +@HWI-ST575:165:C1P5BACXX:1:1101:16660:6380 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJJJJJJJJJJIJJJJJJHHFFDDDDDDDDDDDDDDDDDDCDBDDDBCEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17964:6393 +CCGTCTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIJHEGHG<FGEBADA/@EBHB6<8;>BBB@@@BB00??>@507;<<<>CCDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:18173:6282 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIHJJIJJIGIJIJIIIJHHFFDDDDDDDDDDDDDBDDDCDDBBDDBCEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:18209:6492 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIGIJJHJJJJJJJJIFHFFDDBDDBDDBDDDDDDDDCDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18546:6430 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJHIJJJJJJJJJJJJIGFFFDDDDDDDDDDDDD<@DDCDDBDDDDBDEDDDDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:19023:6315 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIIJJIIIGFHJJJIJBHEHGHFFDDD@BDBDDDD57@CCDDDD<@8<ACDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:19100:6466 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +GGHJBHGGHGGIJJIJIJIG8=DDDBDB@=B=9BDB5@@CCDDB<@BDCDDDDDCCDDCCADDE4++(+,4 +@HWI-ST575:165:C1P5BACXX:1:1101:20025:6400 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJIGBHGIICHD@BFEFIGHBEDDDDBDDBDDDDDD5<B>@CD;BDDDDECDDCCDDBCDADAC::434+:4:::? +@HWI-ST575:165:C1P5BACXX:1:1101:20043:6415 +CCGTCTGTGTCCGAGATCGGAAGATCGTCGT ++ +FHGGG@F0??4?F(<:@FGG@DHCEHH3;B> +@HWI-ST575:165:C1P5BACXX:1:1101:1982:6500 +CCGGTGTCCAGATCGGAAGAGAGTCGT ++ +JFGE8@?FHGGCBCEIIGIDHHIHEEH +@HWI-ST575:165:C1P5BACXX:1:1101:1934:6651 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJIGIJJJJIEIJGIJIJIHHFFDDDDDDDDDDDDDDDDDCDD@BDDDDEDDDDDCCBDDDEEE:44:4+:4+4>0 +@HWI-ST575:165:C1P5BACXX:1:1101:3031:6587 +GATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JHIIJIJJIJJGIIGHHFGIIHFFFDDDD@DDDD<:?CDDDDDDDDDDDDDDDDDDDDDEEE>++:44: +@HWI-ST575:165:C1P5BACXX:1:1101:3659:6632 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACT ++ +JJJHIJIIJHGIJJJJEHJHHFFA@BBB@BD@DDD<BDDCCDBDDDDDDEDDDDDCDBDCDEED:(4444::: +@HWI-ST575:165:C1P5BACXX:1:1101:4271:6639 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJIGHJIJIGJJGIGIEGIGHFEDDDDDDDD=BBDBDDBDCDDDDDDBDECDDDDDDBC@A@CD4+++,+4+44@? +@HWI-ST575:165:C1P5BACXX:1:1101:5521:6516 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JIJAEGIGGGGHIGGGJGEHHFFBBDDDDDDBBBDDDDBCDDDBDDDDDEDCCDDDDBCCC@DE:44:4+4+4(++ +@HWI-ST575:165:C1P5BACXX:1:1101:6888:6616 +CCCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCAC ++ +G???H@?6*B80??F=6A8;4:9B/99@85232550707?3:>@D0078344 +@HWI-ST575:165:C1P5BACXX:1:1101:6970:6733 +CCGATTTGGATCCGCCGAAGCGGATCGGTCAAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIFEGHIIGGGEHGEGHGHGEHDFC>@>@BB>CCDBDBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9786:6627 +CCGATTTGGATCCGCCGAAGCGGATCAGTCGAGGGGTTTACCGCGTTCCACTCTAAC ++ +JJJIJJJJJJIJJJJJJIJFHFFDDDDDDDDBDDDDDBDDDDDDDDDBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10466:6616 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJEHJJJJJIJIJJJJGGHBEBFCC@DBBDDBDDDD9@DCDDDDDDDBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12094:6626 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIJJJJJJJJJJJIIIHFFFDDDDDDDDDDDDDDDDDDDDDDDBDDEDDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13236:6588 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJHJJJJJJJJJIJJIJJHHFFDDDDDDDDDDDDBDDDDDCDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13123:6721 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCCGTTCCACTCTCAC ++ +JJGFHGIIJJGIJIIJIIJFHFFCDDDDDBDBBDBB<B@CDDDDBBCCEDDDDCCC +@HWI-ST575:165:C1P5BACXX:1:1101:13878:6710 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJIIJJJGIIGGIIGEEHEFFCDDDDDDDBDDDDBDDDDDDBDDDDDEDDDDDCC +@HWI-ST575:165:C1P5BACXX:1:1101:14181:6588 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJHIIJIIJJJJIJJJIJHHFFDDDDDDDDDDDDDD7BCDDDDDDB<CDCCCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14627:6506 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IBFIBFAGFBBFEHHGIIFHHB>BBBDDBBD=BDD@5<@?:@B@;<B<9@@C?CDCD +@HWI-ST575:165:C1P5BACXX:1:1101:15075:6709 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIGHIIHIGIEGDHIEFEG/4ABE<BCCBB?BBDD5:BC>><BD@B7<>@><C@@C +@HWI-ST575:165:C1P5BACXX:1:1101:16741:6696 +CCGTCTACACGTATAAGCAGTGTCAATGCGAACGGATC ++ +IJJJJI=FHJJJGIIJIIJGIJJJJGIIGJJHFFDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:16765:6674 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTG ++ +IJJJJJJJJJJJJIIJJGIHHFFDDDDDDDDBDDDDDDDDDDBDDDDDDEDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19358:6651 +CCGCGTATCATAGATCGGAAGAGCGTCGTGT ++ +GGEIFFEHIGCH=CFGCGHIHFGDFFDDBDA +@HWI-ST575:165:C1P5BACXX:1:1101:19561:6627 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJ>HII@GIIGJFGGGIJH8=DDBB@BBDDBDDDB07?>ACDBBD@BCDDDDDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:20819:6735 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJJIJIJJJEEIGIJGGGHHHFFDDDDDBDDDDDDDDBDACDDBBDDDCDDDCDDDCBCC@DDE:+,+4+:+4:@? +@HWI-ST575:165:C1P5BACXX:1:1101:1564:6811 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HIBEDH;DCFHHIIJIJGGBGGAED<ADDBBBB/8<@BBDDCBDBDB?CDDCDDC4> +@HWI-ST575:165:C1P5BACXX:1:1101:1927:6913 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJE<DHIIIIEHIIIIIJFEFFDD?BDDDDBDDDBDBBCCCBBDD@BCDDD9C@@C +@HWI-ST575:165:C1P5BACXX:1:1101:2572:6857 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJJJJJJJJJJJJJJIJJHHFFDDDDDDDDDDDDBDDDDDDDDDDDDDEDCDDDDDDDDDEEE:44:4+:4+:@8 +@HWI-ST575:165:C1P5BACXX:1:1101:3215:6869 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJIJJJIJJJJJJJIIJEEFFDDBBDBDDDDDDDDDDDCDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:3121:6916 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +FDGBDGGICCEGDFGFHGIBEEDDB;>@BBDD8<BBDD?AC:5<BDDDCEAC?CCCC +@HWI-ST575:165:C1P5BACXX:1:1101:5462:6854 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCGT ++ +JJHIJIIJEIIJIJIJIIIGHFFDDDBDDDBDDBB@<BDDDDDDDB<CDDDDDDDCDDDCDDE:+,+,+::+4><< +@HWI-ST575:165:C1P5BACXX:1:1101:6911:6752 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJJJIJJJIJHHFFDDBDDDDDBDDDBDDDDDDDDDDDDDEDDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:8402:6992 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HGE?FD;GB<BD3CHBF<GAABDCB@BD<B@65@B@@@?CDCBBDDDDDECDCC:@A +@HWI-ST575:165:C1P5BACXX:1:1101:10199:6779 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIGIJJJJJJJIJJJIEIHHFFDBDDBDDD?BDDBDDDDDCDDDDDDDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:11749:6891 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GIB@HGIJJIIGGHIIGBH<BEFCD@BB8@B=BDBBBB@CDDDDDDDBDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:12769:6834 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJJJIJJJJJHHFFDDDDDDDDDDDDDDDDDDDDDDDDBDEDDCCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13711:6990 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIIIGHDHHGIIGHIBGEABBDDDDDD?BB@BDDBBDDCCDDDDDD?CDCDDDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:14111:6893 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGIJJJJJJJIJJJIJJHHFFDDDDDDDD=BDD<@DDDDDDDDDDBCEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14440:6994 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJGFHIJIJIJJJJJJIJJFHFFDDDDBBDD?BDD@:BDDDDDDDDDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15694:6869 +CCGGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +IFIGDGGHIJGIIIIGJIB=D<>CDDDD?8@87@<B5:B?:ABB<BDB<ACA +@HWI-ST575:165:C1P5BACXX:1:1101:16093:6801 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJHIJJJJIIIIGIIIEHGIHHFFCDDDDDDDDDDD:BCDDDDDDD@DCDCCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:16736:6834 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIJHIJJJJJJJJJIJJGIHHFFDDDDDDBDDDDDBBDDCDDDDDDDDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:16688:6982 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTC ++ +GFHGJCFHCHGDH=@C;AGHFDDDDB>??BBBDD@75@@ACCDBBBBBA:AACCA +@HWI-ST575:165:C1P5BACXX:1:1101:17036:6768 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIIIGGGIGEIIJJDIIFIEEFEDCBDDDDDDDDD@BDDCDCDDDDDDCEDD?CCDD +@HWI-ST575:165:C1P5BACXX:1:1101:17542:6830 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJIIIJJJIIHHFFDDBDDDDDDDDDDBDDCCCDBDDDBCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18093:6840 +CCGATTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +GIIIGGIJ3DHDH=FE/@EEDFAA8?BDBBDDDB@)5?9:>@5<B<@ADCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18333:6967 +CCGATGTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +B;C@6)0??99B)/7(<'-5-:9/='9>(88;;-750023>307;@B?C@CA +@HWI-ST575:165:C1P5BACXX:1:1101:19230:6988 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJIJIJJIHGFGFFDDDDD@BDDDDDDBDDCDDDB@BDDDCCCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20486:6800 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAG ++ +JJJJIJJJJJJJJIJJIJIHHFFDDDDBBDDDDDDDBDD?CDDBDDDDDEDDDDDDDDDD@DDD:++44+ +@HWI-ST575:165:C1P5BACXX:1:1101:20297:6938 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JID@GIJJHGHGGIGI;ABEFBBDDDDDBDBDDDD@5@DDDDDDDDBDCAC<ACCC +@HWI-ST575:165:C1P5BACXX:1:1101:20449:6990 +CCGATTTGGATCCGCCAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJIJJJJIHIJIJIGGHIJGHFFDCDDDBDDDDD9BCDCBDDDBBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:1103:7035 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIIJJJJIJJJFHJJJJHHFFDD@DDBDDBDDDB@BDDCDDDDDBBCCDDDCCCCDCCCDCC444>:4>:4:@8 +@HWI-ST575:165:C1P5BACXX:1:1101:1500:7064 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJIGIJIIJJJJHHFFDDBDDBDDDDDDBDDDDDDDDDDDBDEDDDDCCC +@HWI-ST575:165:C1P5BACXX:1:1101:1577:7179 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJDEGHHJJJJI@HIIGEGH:B<A?>?88;=;9BD@:<@CDCD;<B<0+:>A9@(4@ +@HWI-ST575:165:C1P5BACXX:1:1101:2297:7148 +CCGTCTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIGIJIJIHIJIJIGGIBEADBCBDDDDDDBDDD97@CCD@DB;;?CCCDDDDDD
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/trimming_stats.txt Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,1 @@ +No trimming: 534 , out of which not recognized MD field for: 0 ; 1 nt trimmed: 90 ; 2 nt trimmed: 14 ; 3 nt trimmed: 9
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/unique_barcodes.txt Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,108 @@ +RNase_P 110 187 2 +RNase_P 111 187 4 +RNase_P 112 187 2 +RNase_P 115 187 1 +RNase_P 117 187 5 +RNase_P 117 188 1 +RNase_P 119 186 1 +RNase_P 134 188 1 +RNase_P 137 187 1 +RNase_P 138 187 1 +RNase_P 152 187 1 +RNase_P 10 187 1 +RNase_P 11 187 1 +RNase_P 15 187 2 +RNase_P 30 187 2 +RNase_P 31 186 1 +RNase_P 31 188 1 +RNase_P 18 186 1 +RNase_P 18 187 1 +RNase_P 32 187 1 +RNase_P 19 187 1 +RNase_P 33 187 1 +RNase_P 50 187 2 +RNase_P 51 187 1 +RNase_P 38 187 1 +RNase_P 39 187 1 +RNase_P 55 187 3 +RNase_P 56 187 1 +RNase_P 2 187 2 +RNase_P 57 187 2 +RNase_P 3 185 1 +RNase_P 3 186 9 +RNase_P 3 187 94 +RNase_P 3 188 1 +RNase_P 58 187 3 +RNase_P 71 187 2 +RNase_P 59 187 1 +RNase_P 4 185 1 +RNase_P 4 186 32 +RNase_P 4 187 289 +RNase_P 73 187 1 +RNase_P 5 187 14 +RNase_P 6 186 2 +RNase_P 6 187 10 +RNase_P 7 187 4 +RNase_P 8 187 4 +RNase_P 9 187 3 +RNase_P 77 187 4 +RNase_P 90 187 2 +RNase_P 78 187 2 +RNase_P 92 187 2 +RNase_P 93 187 1 +RNase_P 94 187 2 +RNase_P 97 186 1 +RNase_P 97 187 4 +RNase_P 100 187 1 +RNase_P 101 187 2 +RNase_P 98 187 1 +RNase_P 99 187 5 +RNase_P 102 187 1 +RNase_P 103 187 1 +RNase_P 104 187 1 +RNase_P 105 187 1 +RNase_P 106 187 2 +RNase_P 107 186 1 +RNase_P 107 187 3 +RNase_P 121 187 2 +RNase_P 109 187 2 +RNase_P 125 187 1 +RNase_P 126 187 2 +RNase_P 128 187 3 +RNase_P 129 187 1 +RNase_P 142 187 1 +RNase_P 145 187 1 +RNase_P 147 187 1 +RNase_P 148 187 1 +RNase_P 163 187 1 +RNase_P 169 187 1 +RNase_P 20 187 1 +RNase_P 21 186 2 +RNase_P 21 187 7 +RNase_P 22 187 2 +RNase_P 23 185 1 +RNase_P 23 187 4 +RNase_P 24 187 1 +RNase_P 25 187 1 +RNase_P 27 186 1 +RNase_P 27 187 1 +RNase_P 40 186 1 +RNase_P 40 187 7 +RNase_P 41 187 1 +RNase_P 42 187 1 +RNase_P 43 187 1 +RNase_P 44 186 1 +RNase_P 44 187 1 +RNase_P 45 187 2 +RNase_P 48 186 1 +RNase_P 63 187 1 +RNase_P 64 187 1 +RNase_P 65 187 1 +RNase_P 81 187 2 +RNase_P 68 187 1 +RNase_P 82 187 2 +RNase_P 83 187 1 +RNase_P 85 187 2 +RNase_P 86 187 1 +RNase_P 89 147 1 +RNase_P 89 187 6
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_dependencies.xml Wed Nov 05 09:09:03 2014 -0500 @@ -0,0 +1,18 @@ +<?xml version="1.0"?> +<tool_dependency> + <package name="gnu_awk" version="4.1.0"> + <repository changeset_revision="cbe9f1c8c98b" name="package_gnu_awk_4_1_0" owner="iuc" toolshed="https://testtoolshed.g2.bx.psu.edu" /> + </package> + <package name="samtools" version="0.1.19"> + <repository changeset_revision="632f1a03db92" name="package_samtools_0_1_19" owner="iuc" toolshed="https://testtoolshed.g2.bx.psu.edu" /> + </package> + <package name="R_3_1_1" version="3.1.1"> + <repository changeset_revision="d9964efbfbe3" name="package_r_3_1_1" owner="fubar" toolshed="https://testtoolshed.g2.bx.psu.edu" /> + </package> + <package name="RNAprobR" version="1.0.0"> + <repository changeset_revision="cabd73083325" name="package_rna_probing_0_99_0" owner="nikos" toolshed="https://testtoolshed.g2.bx.psu.edu" /> + </package> + <set_environment version="1.0"> + <environment_variable action="set_to" name="RNA_PROBING_SCRIPT_PATH">$REPOSITORY_INSTALL_DIR</environment_variable> + </set_environment> +</tool_dependency>