changeset 0:a903407e3ca0 draft default tip

planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/deseq2 commit f95b47ed1a09ce14d3b565e8ea56d8bf12c35814-dirty
author mvdbeek
date Sat, 05 Mar 2016 07:05:06 -0500
parents
children
files deseq2.xml test-data/counts.tab test-data/dge.tab test-data/dge.tab.re_match test-data/dge.tab.re_match.modified tool_dependencies.xml
diffstat 6 files changed, 2065 insertions(+), 0 deletions(-) [+]
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/deseq2.xml	Sat Mar 05 07:05:06 2016 -0500
@@ -0,0 +1,56 @@
+<tool id="DESeq2" name="DESeq2 Profiling" version="1.0.2" hidden="true">
+	<description>of readcount lists</description>
+        <requirements>
+          <requirement type="package" version="3.1.2">R</requirement>
+          <requirement type="package" version="2.14">biocbasics</requirement>
+        </requirements>
+	<command>Rscript $DESeq2 </command>
+	<inputs>
+		<param name="input" type="data" format="tabular" label="miR hit lists, more thant 2 samples"/>
+                <param name="expPlan" type="text" label="experimental plan" help="Use a string of Cs and Ts. exemple: CCCTTT means 3 control samples versus 3 test samples"/>
+	</inputs>
+  	<outputs>
+		<data name="output" format="tabular" label="DESeq2 differential calling" />
+	</outputs>
+        <tests>
+            <test>
+                <param name="input" value="counts.tab" ftype="tabular"/>
+                <param name="expPlan" value="CCTTT"/>
+                <output name="output" file="dge.tab.re_match.modified" ftype="tabular" compare="re_match" lines_diff="50"/>
+            </test>
+        </tests>
+<configfiles>
+    <configfile name="DESeq2">
+      ## Setup R error handling to go to stderr
+      options( show.error.messages=F,  error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } )
+      suppressMessages(require(DESeq2))
+      ## suppressMessages(require(ReportingTools))
+      countData = read.delim("${input}", header=TRUE, check.names=FALSE)
+      rownames( countData )= countData[,1]
+      countData= countData[ , -1 ]
+      stringconds = "${expPlan}"
+      conds = unlist(strsplit(stringconds, split=""))
+      colData=data.frame(row.names=colnames(countData), condition=conds)
+      dds =  DESeqDataSetFromMatrix(countData = countData, colData = colData, design = ~ condition)
+      colData(dds)\$condition = factor(colData(dds)\$condition, levels=c("C","T"))
+      dds = DESeq(dds, quiet=TRUE)
+      res = results(dds)
+      res = res[order(res\$padj),]
+      baseMeanA =  rowMeans(counts(dds, normalized=TRUE)[rownames(res),colData(dds)\$condition== "C"])
+      baseMeanB =  rowMeans(counts(dds, normalized=TRUE)[rownames(res),colData(dds)\$condition== "T"])
+      res2 = data.frame (gene=rownames(res), baseMeanA=baseMeanA, baseMeanB=baseMeanB, res)
+      ## resNA = res[-which(is.na(res[,8])),] ## omit the NA lignes
+      write.table ( res2, file = "${output}", row.names=FALSE, col.names=TRUE, quote= FALSE, dec = ".", sep = "\t", eol = "\n")
+      ## write.csv(as.data.frame(res), file="${output}")
+    </configfile>
+  </configfiles>
+  <help>
+
+**What it does**
+
+DESeq2 differential calling (order by padj, ascending).
+Still in development and testing for replicates/no replicates
+
+
+  </help>
+</tool>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/counts.tab	Sat Mar 05 07:05:06 2016 -0500
@@ -0,0 +1,500 @@
+Geneid	2: Oocyte 1: accepted_hits	3: Oocyte 2: accepted_hits	4: Pronuclei 1: accepted_hits	5: pronuclei 2: accepted_hits	6: Pronuclei 3: accepted_hits
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/dge.tab	Sat Mar 05 07:05:06 2016 -0500
@@ -0,0 +1,500 @@
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+NM_175443	0	0	0	NA	NA	NA	NA	NA
+NM_007495	0	0	0	NA	NA	NA	NA	NA
+NM_022312	0	0	0	NA	NA	NA	NA	NA
+NM_001205204	0	0	0	NA	NA	NA	NA	NA
+NM_001159525	0	0	0	NA	NA	NA	NA	NA
+NM_023041	1.37372077356846	3.62948389222848	2.72717864476447	0.241164141499496	0.649267368058022	0.371440416327753	0.710309527351248	NA
+NM_011880	0	0	0	NA	NA	NA	NA	NA
+NM_026626	17.7633785382913	1.53399458748513	8.02574816780758	-1.09117512175248	0.772317520705131	-1.41285817361262	0.157697429392853	NA
+NM_001199003	0	0	0	NA	NA	NA	NA	NA
+NM_001243792	0	0.280747358629103	0.168448415177462	0.236266271681685	0.47261510198017	0.499912657661113	0.617136579212974	NA
+NM_001159750	0	0	0	NA	NA	NA	NA	NA
+NM_001159751	1.41331154517836	0	0.565324618071345	-0.456463591926576	0.529518721139788	-0.862034851089002	0.388668339981602	NA
+NM_001195662	0	0.280747358629103	0.168448415177462	0.236266271681685	0.47261510198017	0.499912657661113	0.617136579212974	NA
+NM_001177795	0	0	0	NA	NA	NA	NA	NA
+NM_011541	0	0	0	NA	NA	NA	NA	NA
+NM_021374	0	0	0	NA	NA	NA	NA	NA
+NM_001290372	0	0	0	NA	NA	NA	NA	NA
+NM_001244693	0	0	0	NA	NA	NA	NA	NA
+NM_001244692	0	0	0	NA	NA	NA	NA	NA
+NR_045189	0	0	0	NA	NA	NA	NA	NA
+NR_045188	0	0	0	NA	NA	NA	NA	NA
+NM_173868	0	0	0	NA	NA	NA	NA	NA
+NM_133220	0	0	0	NA	NA	NA	NA	NA
+NM_145692	6.3579092478396	7.60358749675086	7.10531619718636	0.083387996518169	0.775018331669092	0.107594869838218	0.914317066643054	NA
+NR_110971	0	0	0	NA	NA	NA	NA	NA
+NM_177547	0	0	0	NA	NA	NA	NA	NA
+NM_029525	0	2.3009918812277	1.38059512873662	0.347864740685333	0.549154085859992	0.633455617726245	0.526436154936629	NA
+NM_001033636	16.0135454344287	12.4994761751687	13.9051038788727	-0.201934666258344	0.819525090034681	-0.246404495376461	0.805369126695018	NA
+NM_001077695	0	0	0	NA	NA	NA	NA	NA
+NM_001302702	0	0	0	NA	NA	NA	NA	NA
+NM_001081209	1.80523385035134	5.47920368753355	4.00961575266067	0.360542139167825	0.715601263912174	0.503831054177784	0.614380103337573	NA
+NM_172294	0	0	0	NA	NA	NA	NA	NA
+NM_001198565	0	0	0	NA	NA	NA	NA	NA
+NM_001198566	0	0	0	NA	NA	NA	NA	NA
+NM_008678	3.19875000972475	5.5548456001552	4.61240736398302	0.234540698172975	0.761871208446781	0.307848223653354	0.758197825122574	NA
+NM_010164	13.3254900447067	14.2582142411885	13.8851245625957	0.0492917614007714	0.823935581191668	0.0598247757785628	0.952295192696345	NA
+NM_001252192	0	0	0	NA	NA	NA	NA	NA
+NM_025303	2.33572385615899	3.56131332270023	3.07107753608373	0.148263520675366	0.713588689874672	0.207771679651209	0.835407246411712	NA
+NM_001033288	46.7817024336213	15.2482361874094	27.8616226858942	-1.00203403042916	0.804072618456076	-1.24619842465622	0.212691557278712	NA
+NM_001111272	0	0	0	NA	NA	NA	NA	NA
+NM_020604	24.6121870203286	20.2356340297381	21.9862552259743	-0.148455582496521	0.824214512448823	-0.180117651720843	0.857060205167467	NA
+NR_130314	8.2621200272157	4.81495768203316	6.19382262010618	-0.292239764573046	0.794908818976628	-0.367639353843473	0.713142166654431	NA
+NM_153154	4.67144771231797	6.56033766718846	5.80478168524026	0.168438897426745	0.783063710694909	0.215102417755087	0.829687468931058	NA
+NM_001077353	0	0	0	NA	NA	NA	NA	NA
+NM_001288617	0	0	0	NA	NA	NA	NA	NA
+NM_010356	0	0	0	NA	NA	NA	NA	NA
+NM_023872	0	0	0	NA	NA	NA	NA	NA
+NM_001160139	0	0	0	NA	NA	NA	NA	NA
+NM_183018	0	0	0	NA	NA	NA	NA	NA
+NM_001012623	0	0	0	NA	NA	NA	NA	NA
+NM_053270	0	0	0	NA	NA	NA	NA	NA
+NM_001012624	0	0	0	NA	NA	NA	NA	NA
+NM_001012625	0	0	0	NA	NA	NA	NA	NA
+NR_110972	0	0	0	NA	NA	NA	NA	NA
+NM_028534	18.9391454538102	17.5808322670669	18.1241575417642	-0.0669263749450893	0.799566757758008	-0.083703298437208	0.933292334577593	NA
+NM_001290683	0	0	0	NA	NA	NA	NA	NA
+NM_007740	0	0	0	NA	NA	NA	NA	NA
+NM_001290691	0	0	0	NA	NA	NA	NA	NA
+NM_001033764	0	0	0	NA	NA	NA	NA	NA
+NM_001013750	0	0	0	NA	NA	NA	NA	NA
+NM_133833	0	0	0	NA	NA	NA	NA	NA
+NM_134448	0	0	0	NA	NA	NA	NA	NA
+NM_001276764	21.8013739048508	17.669234606545	19.3220903258673	-0.18122230083625	0.808923155709414	-0.224029068221345	0.822734684852368	NA
+NM_183019	7.39909387364994	2.09548930474334	4.21693113230598	-0.487829487308654	0.727750130250444	-0.670325523872836	0.502650299726579	NA
+NM_001039551	0	0	0	NA	NA	NA	NA	NA
+NM_053186	0	0	0	NA	NA	NA	NA	NA
+NM_001289766	0	0	0	NA	NA	NA	NA	NA
+NM_009539	0	0	0	NA	NA	NA	NA	NA
+NM_001289765	0	0	0	NA	NA	NA	NA	NA
+NM_001290721	0	0	0	NA	NA	NA	NA	NA
+NM_027111	0	0	0	NA	NA	NA	NA	NA
+NM_207228	0	1.53399458748513	0.920396752491081	0.334952026879345	0.541682645308212	0.618354731835207	0.536341530882536	NA
+NM_001290720	0.942207696785575	0	0.37688307871423	-0.420087470788302	0.514998495489618	-0.815706209760705	0.414668186551214	NA
+NM_001123382	0	0	0	NA	NA	NA	NA	NA
+NM_001252202	0	0	0	NA	NA	NA	NA	NA
+NM_001252200	0	0	0	NA	NA	NA	NA	NA
+NM_001294171	0	0	0	NA	NA	NA	NA	NA
+NM_001252201	0.922412310980623	4.49354625997326	3.0650926803762	0.386876873203666	0.66823515803804	0.578953185192394	0.562620761931149	NA
+NM_008696	0	0	0	NA	NA	NA	NA	NA
+NM_008362	0	0.753442605932299	0.452065563559379	0.301087165819145	0.520525371138935	0.578429376382464	0.562974265503773	NA
+NM_010743	0	0	0	NA	NA	NA	NA	NA
+NM_001025602	0	0	0	NA	NA	NA	NA	NA
+NM_001033289	0.902616925175671	0.561494717258207	0.697943600425192	-0.0811063372188772	0.539075501471879	-0.150454504048925	0.880406042588724	NA
+NR_027826	0	6.23932337560545	3.74359402536327	0.574660493798025	0.652266475310149	0.881021048222319	0.378306427721285	NA
+NM_010879	0.902616925175671	4.91770897027534	3.31167215223548	0.365772177483306	0.644894934792924	0.567181036397434	0.570591190647801	NA
+NM_007737	0	0	0	NA	NA	NA	NA	NA
+NM_010863	0	0	0	NA	NA	NA	NA	NA
+NM_001290982	0	0	0	NA	NA	NA	NA	NA
+NM_001161817	0	0	0	NA	NA	NA	NA	NA
+NM_001142647	0	0	0	NA	NA	NA	NA	NA
+NM_146108	1.80523385035134	1.47977583624406	1.60995904188697	-0.0385599537360845	0.553241223453529	-0.069698265605335	0.94443382227294	NA
+NR_024590	0	0	0	NA	NA	NA	NA	NA
+NM_030025	22.6089993119277	25.9739613909892	24.6279765593646	0.122422955631097	0.8075602877401	0.151596057272317	0.879505543306693	NA
+NM_172655	0	0	0	NA	NA	NA	NA	NA
+NM_001177867	0	0	0	NA	NA	NA	NA	NA
+NM_001164566	0	0	0	NA	NA	NA	NA	NA
+NM_023617	0	4.8092714677932	2.88556288067592	0.362653607126528	0.557338211945436	0.650688575363701	0.515247539548311	NA
+NM_144882	0	0	0	NA	NA	NA	NA	NA
+NM_028717	0	0	0	NA	NA	NA	NA	NA
+NM_001159948	0	0	0	NA	NA	NA	NA	NA
+NM_175200	18.3532547015138	14.2849264863321	15.9122577724048	-0.154092267041592	0.815160114237154	-0.189033129013918	0.85006685166041	NA
+NM_139150	0	0	0	NA	NA	NA	NA	NA
+NM_001285463	0	0.73988791812203	0.443932750873218	0.300229497448123	0.519958206628411	0.577410825756392	0.563661962781561	NA
+NM_001077404	0	0	0	NA	NA	NA	NA	NA
+NM_001077405	0	0	0	NA	NA	NA	NA	NA
+NM_001077406	0	0	0	NA	NA	NA	NA	NA
+NM_001077407	0	0	0	NA	NA	NA	NA	NA
+NM_010939	0	0	0	NA	NA	NA	NA	NA
+NM_001077403	0	0	0	NA	NA	NA	NA	NA
+NR_045915	11.1877200465972	15.957243948673	14.0494343878427	0.281639416666463	0.820949498236479	0.343065459290086	0.731549215475105	NA
+NM_001285937	0	0	0	NA	NA	NA	NA	NA
+NM_001285936	0	0	0	NA	NA	NA	NA	NA
+NM_001081658	0	0	0	NA	NA	NA	NA	NA
+NR_045914	0	0.561494717258207	0.336896830354924	0.286552408470409	0.510690894121541	0.561107338644286	0.574724365627878	NA
+NM_011780	0	1.32849201100077	0.797095206600464	0.32992045736263	0.538685692063126	0.612454465050036	0.540237116051552	NA
+NM_001177600	0	0	0	NA	NA	NA	NA	NA
+NM_009952	0	0	0	NA	NA	NA	NA	NA
+NM_001037726	0	0	0	NA	NA	NA	NA	NA
+NM_133828	0	0	0	NA	NA	NA	NA	NA
+NM_139270	2.70785077552701	0	1.0831403102108	-0.500684404220749	0.545236586098385	-0.918288348556279	0.358467925574221	NA
+NM_175510	89.1810487889722	161.709202258668	132.69794087079	0.739982399197666	0.564835089112411	1.31008574619626	NA	NA
+NM_021285	0	0	0	NA	NA	NA	NA	NA
+NM_008632	0	0	0	NA	NA	NA	NA	NA
+NM_001039934	0	0	0	NA	NA	NA	NA	NA
+NM_177645	2.76723693294187	2.80787071676793	2.79161720323751	0.00681688274066695	0.699730993275294	0.00974214777704579	0.992227013654797	NA
+NM_001122738	0	0	0	NA	NA	NA	NA	NA
+NM_001113387	0	0	0	NA	NA	NA	NA	NA
+NM_001276408	0	0	0	NA	NA	NA	NA	NA
+NM_001276412	0	0	0	NA	NA	NA	NA	NA
+NM_001276411	0	0	0	NA	NA	NA	NA	NA
+NM_001276410	0	0	0	NA	NA	NA	NA	NA
+NM_001276409	0	0	0	NA	NA	NA	NA	NA
+NM_010233	0	0	0	NA	NA	NA	NA	NA
+NM_001276413	0	0	0	NA	NA	NA	NA	NA
+NM_008342	0	0.561494717258207	0.336896830354924	0.286552408470409	0.510690894121541	0.561107338644286	0.574724365627878	NA
+NM_176980	3.15915923811485	6.03461506007489	4.88443273129087	0.207445396740765	0.699195143577849	0.296691701374307	0.766701893269917	NA
+NM_001170641	0	0	0	NA	NA	NA	NA	NA
+NM_027884	0	0	0	NA	NA	NA	NA	NA
+NR_045311	12.6366369524594	6.74031938995988	9.09884641495969	-0.288663762643429	0.763714138569315	-0.377973574227904	0.705450225003955	NA
+NM_001034060	0	0	0	NA	NA	NA	NA	NA
+NM_001289895	0	0	0	NA	NA	NA	NA	NA
+NM_009518	0	0	0	NA	NA	NA	NA	NA
+NM_001039495	0	0	0	NA	NA	NA	NA	NA
+NM_001159884	0	0	0	NA	NA	NA	NA	NA
+NM_001159885	0	1.84971979530508	1.10983187718305	0.341530939138289	0.545528223441631	0.626055489821657	0.531278545545514	NA
+NM_001159883	0	0.383498646871284	0.23009918812277	0.253165513151483	0.486165361864932	0.520739511717451	0.602548245637964	NA
+NM_178055	0	0	0	NA	NA	NA	NA	NA
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+NR_037976	0	0	0	NA	NA	NA	NA	NA
+NM_008781	9.02616925175671	0.73988791812203	4.0544004515759	-0.585513841147185	0.63537910546698	-0.921518879215982	0.356779589173685	NA
+NM_026713	0	0	0	NA	NA	NA	NA	NA
+NM_001159520	0	0	0	NA	NA	NA	NA	NA
+NM_001285927	0	0	0	NA	NA	NA	NA	NA
+NM_029777	0	0	0	NA	NA	NA	NA	NA
+NM_007735	0	0	0	NA	NA	NA	NA	NA
+NM_172430	0	13.9766726216056	8.38600357296334	1.08532277460524	0.765742470992951	1.41734697462697	0.156381507508417	NA
+NM_001081746	10.2653077356166	12.1701962795385	11.4082408619697	0.122684192771473	0.825130466380477	0.148684599309053	0.881802514119701	NA
+NM_001144993	0	0	0	NA	NA	NA	NA	NA
+NM_001144992	0	0	0	NA	NA	NA	NA	NA
+NM_001172157	0	0	0	NA	NA	NA	NA	NA
+NM_001081082	0	0	0	NA	NA	NA	NA	NA
+NM_153530	0	0	0	NA	NA	NA	NA	NA
+NM_201644	0	0	0	NA	NA	NA	NA	NA
+NM_001110192	0	0	0	NA	NA	NA	NA	NA
+NM_001205391	0	0	0	NA	NA	NA	NA	NA
+NM_019867	0	0.561494717258207	0.336896830354924	0.286552408470409	0.510690894121541	0.561107338644286	0.574724365627878	NA
+NM_001205392	0	0	0	NA	NA	NA	NA	NA
+NM_201410	0	0	0	NA	NA	NA	NA	NA
+NM_029846	0	0	0	NA	NA	NA	NA	NA
+NM_001110212	0	0	0	NA	NA	NA	NA	NA
+NM_001111314	0	10.2921757171822	6.17530543030933	0.862182867065928	0.729016942588022	1.18266506126067	0.236941916299098	NA
+NM_146112	0	0	0	NA	NA	NA	NA	NA
+NM_010566	0	0	0	NA	NA	NA	NA	NA
+NR_122078	0	0	0	NA	NA	NA	NA	NA
+NM_201642	0	0	0	NA	NA	NA	NA	NA
+NM_201641	0	0	0	NA	NA	NA	NA	NA
+NM_001110193	0	0	0	NA	NA	NA	NA	NA
+NM_145079	0	0	0	NA	NA	NA	NA	NA
+NM_029269	0	0	0	NA	NA	NA	NA	NA
+NM_139152	0	0	0	NA	NA	NA	NA	NA
+NM_178119	0.942207696785575	3.45816490398115	2.45178202110292	0.23912879124041	0.590086443595563	0.405243661900333	0.685298413793502	NA
+NM_001037136	0	0	0	NA	NA	NA	NA	NA
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+NM_001243009	0	0	0	NA	NA	NA	NA	NA
+NM_001243008	0	0	0	NA	NA	NA	NA	NA
+NM_023046	0	0	0	NA	NA	NA	NA	NA
+NM_001039126	0	0	0	NA	NA	NA	NA	NA
+NM_007855	0	0	0	NA	NA	NA	NA	NA
+NM_001271884	0	0	0	NA	NA	NA	NA	NA
+NM_207031	0	1.04774465237167	0.628646791423002	0.31923308341291	0.532154371221071	0.59988811645088	0.548580802705268	NA
+NM_172463	37.6605416637659	8.27272545553742	20.0278519388288	-1.36450538728293	0.80057392783942	-1.70440897440346	0.0883047084715896	NA
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+NR_040467	0	0	0	NA	NA	NA	NA	NA
+NM_028942	0	0	0	NA	NA	NA	NA	NA
+NR_120500	13.0879454150472	25.7366540925439	20.6771706215453	0.397553100481943	0.814316545181192	0.488204621205976	0.625404911601836	NA
+NM_027584	0	0	0	NA	NA	NA	NA	NA
+NM_001164233	0	0	0	NA	NA	NA	NA	NA
+NM_013626	4.16075309231528	5.46445760829258	4.94297580190166	0.11114846061368	0.742510223014856	0.14969283542303	0.881006960992266	NA
+NM_173187	0	0	0	NA	NA	NA	NA	NA
+NM_009399	0	1.47977583624406	0.887865501746437	0.333753920102786	0.540973444950313	0.61695065297233	0.537267275055595	NA
+NM_178779	0	0	0	NA	NA	NA	NA	NA
+NM_029349	0	0	0	NA	NA	NA	NA	NA
+NM_001160368	0	0	0	NA	NA	NA	NA	NA
+NM_009741	14.4814615744206	14.9625266561117	14.7701006234353	0.0250257143887851	0.824676761721047	0.0303460889774049	0.975791039793048	NA
+NM_198028	0	0	0	NA	NA	NA	NA	NA
+NM_001160307	0	0	0	NA	NA	NA	NA	NA
+NR_102307	0	0	0	NA	NA	NA	NA	NA
+NR_102306	0	0	0	NA	NA	NA	NA	NA
+NM_172853	0	8.7031681175022	5.22190087050132	0.563197806076453	0.647878985885826	0.869294757733821	NA	NA
+NM_001077425	0	14.6886569031533	8.81319414189195	1.13270305596256	0.771815467334734	1.46758273693848	0.14221760004542	NA
+NM_029709	0	0	0	NA	NA	NA	NA	NA
+NM_001293300	0	0.280747358629103	0.168448415177462	0.236266271681685	0.47261510198017	0.499912657661113	0.617136579212974	NA
+NM_001293301	0	0	0	NA	NA	NA	NA	NA
+NM_022327	0	0	0	NA	NA	NA	NA	NA
+NM_001172074	2.31592847035403	1.87682917092561	2.05246889069698	-0.0512918580082018	0.609262188750539	-0.0841868393530049	0.932907881715578	NA
+NM_133186	0	0	0	NA	NA	NA	NA	NA
+NM_001085409	0	0	0	NA	NA	NA	NA	NA
+NM_028439	0	0	0	NA	NA	NA	NA	NA
+NM_178082	0	0	0	NA	NA	NA	NA	NA
+NM_029955	0	0	0	NA	NA	NA	NA	NA
+NM_133748	0	0	0	NA	NA	NA	NA	NA
+NM_001271532	0	0	0	NA	NA	NA	NA	NA
+NM_001271531	0	0	0	NA	NA	NA	NA	NA
+NM_001025156	0	0	0	NA	NA	NA	NA	NA
+NM_027677	1.80523385035134	2.58960771342711	2.2758581681968	0.0651401260613425	0.556538034797777	0.117045236782445	0.906824206046482	NA
+NM_145100	0	0	0	NA	NA	NA	NA	NA
+NM_001033041	0	0	0	NA	NA	NA	NA	NA
+NM_001285945	0	0	0	NA	NA	NA	NA	NA
+NM_145128	0.902616925175671	24.3191660046423	14.9525463728557	1.95361459558199	0.824630542310642	2.36907863018012	0.017832461848317	NA
+NM_027678	0	0.369943959061015	0.221966375436609	0.251236829594032	0.484659763413011	0.518377733329465	0.604194747223895	NA
+NM_172485	281.810844108409	273.901284979979	277.065108631351	-0.0344125191892855	0.516914896097389	-0.0665728913000837	NA	NA
+NM_026976	0	0	0	NA	NA	NA	NA	NA
+NM_007799	0	0	0	NA	NA	NA	NA	NA
+NM_172510	8.00876542267739	20.284760304162	15.3743623515682	0.611900915963187	0.823917669299397	0.742672403765179	0.457680041250665	NA
+NM_175294	0	0	0	NA	NA	NA	NA	NA
+NM_001145804	0	0	0	NA	NA	NA	NA	NA
+NM_001160318	0	0	0	NA	NA	NA	NA	NA
+NM_001160317	0	0	0	NA	NA	NA	NA	NA
+NM_001160316	0	0	0	NA	NA	NA	NA	NA
+NM_182716	0	1.53399458748513	0.920396752491081	0.334952026879345	0.541682645308212	0.618354731835207	0.536341530882536	NA
+NM_144530	0	0	0	NA	NA	NA	NA	NA
+NM_001276767	0	0	0	NA	NA	NA	NA	NA
+NM_001145300	0	0	0	NA	NA	NA	NA	NA
+NM_053106	0	0	0	NA	NA	NA	NA	NA
+NM_001291015	0	0	0	NA	NA	NA	NA	NA
+NM_177642	0	0.766997293742567	0.46019837624554	0.301930674074754	0.521081592818901	0.579430703820099	0.562298591673325	NA
+NM_030676	0	0	0	NA	NA	NA	NA	NA
+NM_001159769	0	0.383498646871284	0.23009918812277	0.253165513151483	0.486165361864932	0.520739511717451	0.602548245637964	NA
+NM_011210	0	0	0	NA	NA	NA	NA	NA
+NM_001111316	0	0	0	NA	NA	NA	NA	NA
+NM_001268286	0	0	0	NA	NA	NA	NA	NA
+NM_181347	0	0	0	NA	NA	NA	NA	NA
+NM_001166501	0.451308462587835	1.13694125280358	0.862688136717284	0.14483340196651	0.542474828609351	0.2669864007106	0.789479631817819	NA
+NR_040455	0	0	0	NA	NA	NA	NA	NA
+NM_001081027	0	0	0	NA	NA	NA	NA	NA
+NR_040604	0	0	0	NA	NA	NA	NA	NA
+NM_020025	1.88441539357115	0	0.75376615742846	-0.478082733990246	0.537452218826069	-0.88953532471128	0.373715447294592	NA
+NR_110891	0	0	0	NA	NA	NA	NA	NA
+NM_001290269	0	0	0	NA	NA	NA	NA	NA
+NM_153171	0	0	0	NA	NA	NA	NA	NA
+NM_001145807	0	0	0	NA	NA	NA	NA	NA
+NM_153539	0	0	0	NA	NA	NA	NA	NA
+NM_008869	35.653368544439	0	14.2613474177756	-1.91885952308802	0.790573257173068	-2.42717484518699	0.0152169203007714	NA
+NM_001110146	0	0	0	NA	NA	NA	NA	NA
+NM_024458	0	0	0	NA	NA	NA	NA	NA
+NM_001159730	0	0	0	NA	NA	NA	NA	NA
+NR_040603	0	0	0	NA	NA	NA	NA	NA
+NM_021400	0.922412310980623	4.55483922383083	3.10186845869075	0.398948760080105	0.67305191911394	0.592745891885002	0.553351223818174	NA
+NM_001024720	0	0	0	NA	NA	NA	NA	NA
+NM_022018	12.2289046724075	12.0519046511016	12.122704659624	-0.00571325189775561	0.80461082939856	-0.00710064007220263	0.994334556522362	NA
+NM_010877	2.70785077552701	6.73793660709848	5.12590227446989	0.236159941499862	0.655339498277251	0.360362746516388	0.71857588174249	NA
+NR_108050	0	0	0	NA	NA	NA	NA	NA
+NR_046171	0	0	0	NA	NA	NA	NA	NA
+NM_177838	0	0	0	NA	NA	NA	NA	NA
+NM_001277730	0	0	0	NA	NA	NA	NA	NA
+NM_001134741	3.66985385811754	7.77979843828435	6.13582060621763	0.384559430761919	0.797430774550789	0.482248043384769	0.629629751412181	NA
+NM_026683	0	0	0	NA	NA	NA	NA	NA
+NM_023141	4.06177616329052	13.4806387799198	9.71309373326807	0.511865528371584	0.773248932023639	0.661967326656372	0.50799216160275	NA
+NR_027640	0.471103848392788	0	0.188441539357115	-0.343626431501831	0.478911579314015	-0.717515396044581	0.473056140037951	NA
+NM_001159968	0	0	0	NA	NA	NA	NA	NA
+NM_001159965	0	0	0	NA	NA	NA	NA	NA
+NM_001159967	0	0	0	NA	NA	NA	NA	NA
+NM_001159966	0	0	0	NA	NA	NA	NA	NA
+NM_023884	0	0	0	NA	NA	NA	NA	NA
+NR_038031	0	0	0	NA	NA	NA	NA	NA
+NR_038030	0	0	0	NA	NA	NA	NA	NA
+NM_001162896	0	0	0	NA	NA	NA	NA	NA
+NM_001038619	0	0	0	NA	NA	NA	NA	NA
+NM_172646	0	0	0	NA	NA	NA	NA	NA
+NM_001293313	0	0	0	NA	NA	NA	NA	NA
+NM_138314	0	0	0	NA	NA	NA	NA	NA
+NM_001081126	0.942207696785575	0.766997293742567	0.83708145495977	-0.0390258061465042	0.54270349190546	-0.0719099963950527	0.942673534674585	NA
+NM_001164306	6.31831847622969	6.05345883164822	6.15940268948081	-0.0158292992457534	0.727327354869798	-0.0217636517309144	0.982636489031444	NA
+NM_001160049	0	0	0	NA	NA	NA	NA	NA
+NM_001033344	0	0	0	NA	NA	NA	NA	NA
+NM_198932	0	0	0	NA	NA	NA	NA	NA
+NM_011137	0	0	0	NA	NA	NA	NA	NA
+NM_030724	42.7278970921829	746.296046918467	464.868786987954	3.76305551214874	0.461005878034587	8.16270614203842	NA	NA
+NM_033652	7.33970771623508	41.9683654481833	28.116902355404	1.66404402883696	0.768767950863607	2.16455957479449	0.0304214296407256	NA
+NM_175461	0	0	0	NA	NA	NA	NA	NA
+NM_001160261	0	0	0	NA	NA	NA	NA	NA
+NM_001160262	0	0	0	NA	NA	NA	NA	NA
+NM_183355	0	0	0	NA	NA	NA	NA	NA
+NM_008783	0	0	0	NA	NA	NA	NA	NA
+NM_001291509	0	0	0	NA	NA	NA	NA	NA
+NM_001291508	0	0	0	NA	NA	NA	NA	NA
+NM_027528	0.942207696785575	0	0.37688307871423	-0.420087470788302	0.514998495489618	-0.815706209760705	0.414668186551214	NA
+NM_013489	0	0	0	NA	NA	NA	NA	NA
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/dge.tab.re_match	Sat Mar 05 07:05:06 2016 -0500
@@ -0,0 +1,500 @@
+gene\	baseMeanA\	baseMeanB\	baseMean\	log2FoldChange\	lfcSE\	stat\	pvalue\	padj
+NM\_001290814\	13\.8361846647093\	302\.692276889662\	187\.149839999681\	4\.02298530293272\	0\.475300264296235\	8\.46409229098463\	2\.58155340319795e\-17\	3\.84651457076494e\-15
+NM\_175296\	42\.3873901225727\	938\.523758683365\	580\.069211259048\	3\.98548352659215\	0\.502124973720984\	7\.93723422489391\	2\.06739417563206e\-15\	1\.54020866084588e\-13
+NM\_010472\	166\.814206844757\	1470\.37801982385\	948\.952494632214\	2\.93850691094032\	0\.401906370179809\	7\.31142158713647\	2\.643307621836e\-13\	1\.31284278551188e\-11
+NM\_029342\	102\.585720376899\	642\.349793886348\	426\.444164482568\	2\.47979721531494\	0\.398185092542637\	6\.22775001314096\	4\.73181277129915e\-10\	1\.76260025730893e\-08
+NM\_001033408\	10\.067353877567\	192\.663868743505\	119\.62526279713\	3\.55941286675189\	0\.592903302264483\	6\.0033615146979\	1\.93273620816604e\-09\	5\.75955390033481e\-08
+NM\_008801\	238\.766220843347\	1165\.70420032215\	794\.929008530628\	2\.16023652550394\	0\.376164519001384\	5\.74279714428886\	9\.31252640346199e\-09\	2\.31261072352639e\-07
+NM\_199021\	342\.796872715182\	1102\.82797928137\	798\.815536654893\	1\.6260740249254\	0\.304536884403641\	5\.33949780207956\	9\.32043996944546e\-08\	1\.98392222206768e\-06
+NM\_009255\	27214\.4775715473\	10819\.482196131\	17377\.4803462975\	\-1\.29469303336041\	0\.268165046272498\	\-4\.82797087598358\	1\.3793125924685e\-06\	2\.56896970347259e\-05
+NM\_023645\	2043\.5349277896\	759\.207151422419\	1272\.93826196929\	\-1\.38274254401162\	0\.289726371358106\	\-4\.77258089255026\	1\.81880019099805e\-06\	2\.89691190975949e\-05
+NM\_172849\	25\.633576260334\	208\.094346965569\	135\.110038683475\	2\.6194543990624\	0\.552643837861215\	4\.73985995971644\	2\.1386597991513e\-06\	2\.89691190975949e\-05
+NM\_177445\	251\.161459424046\	889\.432342079833\	634\.123989017518\	1\.72948048577894\	0\.364622239855406\	4\.74321173185918\	2\.10356203684693e\-06\	2\.89691190975949e\-05
+NM\_172841\	76\.8729625733449\	374\.319684236064\	255\.340995570976\	2\.09413011881783\	0\.452738357845928\	4\.62547536016483\	3\.73740237076367e\-06\	4\.64060794369822e\-05
+NM\_175382\	2935\.45700718525\	1227\.02381262979\	1910\.39709045197\	\-1\.22275330623272\	0\.273866519954751\	\-4\.46477833958947\	8\.0151688516042e\-06\	8\.79235349772213e\-05
+NM\_001081011\	77\.0353110707106\	360\.087339644055\	246\.866528214717\	2\.03634056320338\	0\.456752830431055\	4\.45829872861786\	8\.26127174282616e\-06\	8\.79235349772213e\-05
+NM\_001097644\	9785\.54102308009\	2903\.68847368657\	5656\.42949344398\	\-1\.65478533883576\	0\.380204768887394\	\-4\.35235292728761\	1\.3468422003796e\-05\	0\.000133786325237707
+NM\_001014974\	236\.628450845237\	647\.826152044052\	483\.347071564526\	1\.38929354885259\	0\.336202454844838\	4\.13231232798036\	3\.59131966815585e\-05\	0\.000334441644097014
+NM\_174874\	168\.195766771353\	465\.666676876747\	346\.67831283459\	1\.3928111012325\	0\.36672550516055\	3\.79796627622816\	0\.000145888166183563\	0\.00127866686831476
+NM\_173760\	10652\.175633968\	4790\.47314922489\	7135\.15414312214\	\-1\.11426312561516\	0\.297035656003969\	\-3\.75127734025396\	0\.000175935955242489\	0\.00145635874061838
+NM\_175460\	309\.419841869487\	83\.5797478876932\	173\.915785480411\	\-1\.71657978250327\	0\.471918742846035\	\-3\.63744777787584\	0\.000275352997251232\	0\.00215934718897019
+NM\_019570\	1001\.9294894235\	444\.107250098934\	667\.236145828762\	\-1\.12514489644241\	0\.329403206029142\	\-3\.41570718149861\	0\.00063616614927787\	0\.00464819358846719
+NM\_030013\	73\.361471801667\	271\.643353996245\	192\.330601118414\	1\.69474947951718\	0\.499191266553957\	3\.39499024335193\	0\.0006863104627267\	0\.00464819358846719
+NM\_009581\	9\.18453233819633\	79\.6145738440232\	51\.4425572416925\	2\.35539055140344\	0\.692796709020918\	3\.39982930163186\	0\.000674279329052518\	0\.00464819358846719
+NM\_025819\	7436\.56591598075\	4157\.76659247737\	5469\.28632187872\	\-0\.820136402488323\	0\.243431843899217\	\-3\.3690596486952\	0\.000754251042909209\	0\.00488623501710748
+NM\_001277076\	10\.6176392691796\	95\.767407773599\	61\.7075003718312\	2\.33678175827228\	0\.710230094690954\	3\.29017564271068\	0\.00100124862426847\	0\.00621608520900009
+NM\_026719\	949\.926448892427\	455\.046155963654\	652\.998273135163\	\-1\.02094827570642\	0\.318111375253008\	\-3\.2094051176083\	0\.00133009938283553\	0\.00792739232169974
+NM\_133239\	146\.730914425186\	396\.719385405167\	296\.723997013175\	1\.33565408433631\	0\.418546380184067\	3\.19117342204447\	0\.00141696200430467\	0\.00812028225543832
+NM\_028250\	4\.22013924973014\	55\.3711800561511\	34\.9107637335827\	2\.42374020507959\	0\.763934983366706\	3\.17270482155174\	0\.00151025987951588\	0\.00833439711288392
+NM\_001081304\	3655\.36508406539\	2100\.08154990521\	2722\.19496356928\	\-0\.781122772793943\	0\.248818106016245\	\-3\.13933252406859\	0\.00169333176290756\	0\.00901094402404382
+NM\_145519\	32\.6763531894948\	130\.345225947406\	91\.2776768442418\	1\.72412302967034\	0\.560413439822975\	3\.07651977478442\	0\.00209432410593417\	0\.0105405303039005
+NM\_011800\	466\.439713158195\	196\.045737912676\	304\.203328010883\	\-1\.17785775221796\	0\.38334635231647\	\-3\.07256804479931\	0\.00212225442360414\	0\.0105405303039005
+NM\_133684\	4027\.59094501628\	2113\.20936183842\	2878\.96199510956\	\-0\.898133563093956\	0\.302972450482269\	\-2\.96440670319798\	0\.00303267186440641\	0\.0145763905740824
+NM\_001037725\	8983\.50890428911\	5085\.83304406069\	6644\.90338815206\	\-0\.796418123400809\	0\.280083723494551\	\-2\.84350019866935\	0\.00446209815804139\	0\.0207766445483802
+NM\_001003948\	3786\.72269120433\	2018\.28935948658\	2725\.66269217368\	\-0\.872200899069415\	0\.3198755308669\	\-2\.72668839878327\	0\.00639734203165843\	0\.028884968567185
+NM\_001013025\	1275\.34017247936\	2149\.0710928232\	1799\.57872468567\	0\.731326876623722\	0\.275475486889697\	2\.65478023065098\	0\.00793600927010312\	0\.0347716202364891
+NM\_172513\	3647\.63477073224\	5962\.64180181852\	5036\.63898938401\	0\.690815020123889\	0\.26117254162535\	2\.64505225482263\	0\.00816783025689341\	0\.0347716202364891
+NR\_015470\	70\.7288171584337\	186\.47203086882\	140\.174745384666\	1\.26326158623545\	0\.484972310503016\	2\.60481177765632\	0\.00919247436426714\	0\.0370183427101569
+NM\_133832\	6772\.25221029071\	4323\.54681772322\	5303\.02897475022\	\-0\.633142120084047\	0\.242959557911166\	\-2\.60595683301146\	0\.00916180072793548\	0\.0370183427101569
+NM\_028096\	75\.5388325713863\	7\.08097171407003\	34\.4641160569965\	\-2\.04459867473767\	0\.809963914235661\	\-2\.52430835349881\	0\.0115926194434277\	0\.0449593280908612
+NM\_001004173\	164\.517942091383\	51\.2139918210098\	96\.5355719291591\	\-1\.44859342101189\	0\.577105326809181\	\-2\.51010232225921\	0\.0120696182794258\	0\.0449593280908612
+NM\_028759\	121\.778220435247\	317\.827726722269\	239\.407924207461\	1\.24309742768331\	0\.494819506748558\	2\.51222397405402\	0\.0119972918454875\	0\.0449593280908612
+NM\_018872\	3131\.69249478152\	1830\.44306213535\	2350\.94283519382\	\-0\.747611448692685\	0\.303153123666712\	\-2\.46611824298606\	0\.0136586237421861\	0\.0496374375020909
+NM\_026456\	1687\.97108458083\	2665\.53459133329\	2274\.50918863231\	0\.641671769048607\	0\.263799703348508\	2\.43242035871772\	0\.0149982894923404\	0\.0532082174847315
+NM\_001039644\	269\.664974721322\	106\.514295530262\	171\.774567206686\	\-1\.20689336578232\	0\.499805344847487\	\-2\.41472680959544\	0\.0157470155377294\	0\.0545652398865508
+NM\_153179\	66\.0335886493847\	189\.535268529269\	140\.134596577315\	1\.29200422163446\	0\.58476801784504\	2\.20943037616129\	0\.0271447201486578\	0\.0919218932306822
+NM\_013862\	57\.9773933020706\	150\.9751957847\	113\.776074791648\	1\.20034611313879\	0\.555406039747788\	2\.16120464531475\	0\.030679534747105\	0\.0993750147243185
+NM\_031179\	23699\.3093701959\	16327\.064312376\	19275\.962335504\	\-0\.525640098441811\	0\.243036267009764\	\-2\.16280518504134\	0\.0305561666441146\	0\.0993750147243185
+NM\_028450\	159\.031029818961\	64\.3010773045724\	102\.193058310328\	\-1\.14530381054679\	0\.542683945675832\	\-2\.11044350892026\	0\.0348201721775652\	0\.110387354350153
+NM\_011086\	1088\.38700913089\	708\.41277293586\	860\.402467413874\	\-0\.600506525695301\	0\.285809705300589\	\-2\.1010711482444\	0\.0356347211373772\	0\.110616113530608
+NM\_181750\	988\.145510107309\	1633\.42677273327\	1375\.31426768289\	0\.691681110475252\	0\.345636673854412\	2\.00117974392555\	0\.0453730230669608\	0\.137971029326064
+NM\_028320\	66\.3503148222639\	168\.766177639581\	127\.799832512654\	1\.13334103519654\	0\.596856523994828\	1\.89885004123095\	0\.0575841955463193\	0\.168505025189728
+NM\_011273\	1169\.7213776569\	1783\.31452717056\	1537\.8772673651\	0\.586209055891467\	0\.308831652616104\	1\.898150824003\	0\.0576762166756786\	0\.168505025189728
+NM\_009307\	19\.1370993118597\	73\.9052049885975\	51\.9979627179024\	1\.38855981974957\	0\.738731163363936\	1\.87965512843201\	0\.0601550947186711\	0\.172367482943884
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+NM\_008783\	0\	0\	0\	NA\	NA\	NA\	NA\	NA
+NM\_001291509\	0\	0\	0\	NA\	NA\	NA\	NA\	NA
+NM\_001291508\	0\	0\	0\	NA\	NA\	NA\	NA\	NA
+NM\_027528\	0\.942207696785575\	0\	0\.37688307871423\	\-0\.420087470788302\	0\.514998495489618\	\-0\.815706209760705\	0\.414668186551214\	NA
+NM\_013489\	0\	0\	0\	NA\	NA\	NA\	NA\	NA
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/dge.tab.re_match.modified	Sat Mar 05 07:05:06 2016 -0500
@@ -0,0 +1,500 @@
+gene\	baseMeanA\	baseMeanB\	baseMean\	log2FoldChange\	lfcSE\	stat\	pvalue\	padj
+NM\_001290814\	13\.836184664.*?	302\.69227688.*?	187\.14983999.*?	4\.0229853029.*?	0\.47530026429.*?	8\.4640922909.*?	2\.5815534.*?	3\.8465145.*?
+NM\_175296\	42\.387390122.*?	938\.52375868.*?	580\.06921125.*?	3\.9854835265.*?	0\.50212497372.*?	7\.9372342248.*?	2\.0673941.*?	1\.5402086.*?
+NM\_010472\	166\.81420684.*?	1470\.3780198.*?	948\.95249463.*?	2\.9385069109.*?	0\.40190637017.*?	7\.3114215871.*?	2\.64330.*?	1\.3128427.*?
+NM\_029342\	102\.58572037.*?	642\.34979388.*?	426\.44416448.*?	2\.4797972153.*?	0\.39818509254.*?	6\.2277500131.*?	4\.7318127.*?	1\.7626002.*?
+NM\_001033408\	10\.06735387.*?	192\.66386874.*?	119\.6252627.*?	3\.5594128667.*?	0\.59290330226.*?	6\.003361514.*?	1\.9327362.*?	5\.7595539.*?
+NM\_008801\	238\.76622084.*?	1165\.7042003.*?	794\.92900853.*?	2\.1602365255.*?	0\.37616451900.*?	5\.7427971442.*?	9\.3125264.*?	2\.3126107.*?
+NM\_199021\	342\.79687271.*?	1102\.8279792.*?	798\.81553665.*?	1\.626074024.*?	0\.30453688440.*?	5\.3394978020.*?	9\.3204399.*?	1\.9839222.*?
+NM\_009255\	27214\.477571.*?	10819\.48219.*?	17377\.480346.*?	\-1\.2946930333.*?	0\.26816504627.*?	\-4\.8279708759.*?	1\.379312.*?	2\.5689697.*?
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_dependencies.xml	Sat Mar 05 07:05:06 2016 -0500
@@ -0,0 +1,9 @@
+<?xml version="1.0"?>
+<tool_dependency>
+    <package name="R" version="3.1.2">
+        <repository changeset_revision="1ca39eb16186" name="package_r_3_1_2" owner="iuc" prior_installation_required="True" toolshed="https://testtoolshed.g2.bx.psu.edu" />
+    </package>
+    <package name="biocbasics" version="2.14">
+        <repository changeset_revision="d243626801e2" name="package_biocbasics_2_14" owner="mvdbeek" toolshed="https://testtoolshed.g2.bx.psu.edu" />
+    </package>
+</tool_dependency>