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3
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1 # Setup R error handling to go to stderr
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2 options(show.error.messages=F, error=function(){cat(geterrmessage(),file=stderr());q("no",1,F)})
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2
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3
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3
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4 # We need to not crash galaxy with an UTF8 error on German LC settings.
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5 loc <- Sys.setlocale("LC_MESSAGES", "en_US.UTF-8")
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2
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6
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7
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3
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8 # Import library
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9 library("getopt")
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10 library("RColorBrewer")
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11 library("gplots")
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12 options(stringAsfactors = FALSE, useFancyQuotes = FALSE)
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13 # Take in trailing command line arguments
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14 args <- commandArgs(trailingOnly = TRUE)
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2
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15
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16
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3
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17 # get options, using the spec as defined by the enclosed list.
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18 # we read the options from the default: commandArgs(TRUE).
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19 option_specification = matrix(c(
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20 'input', 'i', 2, 'character',
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8
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21 'title', 't', 2, 'character',
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3
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22 'transform', 'c', 2, 'character',
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8
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23 'key', 'k', 2, 'character',
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10
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24 'colorscheme', 'z', 2, 'character',
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13
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25 'cluster', 'b', 2, 'character',
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26 'labels', 'a', 2, 'character',
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3
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27 'output', 'o', 2, 'character'
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28 ), byrow=TRUE, ncol=4);
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2
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29
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3
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30 # Parse options
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31 options = getopt(option_specification);
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2
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32
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33
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34
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3
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35 # Print options to see what is going on
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36 cat("\n input: ",options$input)
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37 cat("\n title: ",options$title)
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38 cat("\n output: ",options$output)
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2
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39
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3
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40 input <- read.delim(options$input,sep='\t',header=TRUE)
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2
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41
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3
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42 mat_input <- data.matrix(input[,2:ncol(input)])
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2
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43
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44
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3
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45 if(options$transform == "none"){
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46 linput <- mat_input
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47 }else if(options$transform == "log2"){
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48 linput <- log2(mat_input)
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49 }else if(options$transform == "log2plus1"){
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50 linput <- log2(mat_input+1)
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51 }else if(options$transform == "log10"){
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52 linput <- log10(mat_input)
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53 }else if(options$transform == "log10plus1"){
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54 linput <- log10(mat_input+1)
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55 }else{
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56 }
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2
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57
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13
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58 if(options$colorscheme == "whrd"){
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10
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59 colorscale = colfunc <- colorRampPalette(c("white", "red"))
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13
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60 } else if(options$colorscheme == "whblu"){
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61 colorscale = colfunc <- colorRampPalette(c("white", "blue"))
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62 }else if(options$colorscheme == "blwhre"){
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10
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63 colorscale = colfunc <- colorRampPalette(c("blue","white", "red"))
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13
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64 }else{
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10
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65 }
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2
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66
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67
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13
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68 if(options$cluster== "Default"){
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69 hclust_fun = function(x) hclust(x, method="complete")
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70 dist_fun = function(x) dist(x, method="maximum")
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71 clust = distfun=dist_fun, hclustfun=hclust_fun
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72 }else{
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73 clust = NULL
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74 }
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75
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76 if(options$labels== "both"){
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77 labs = NULL
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78 }else if(options$labels== "rows"){
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79 labs = labCol=FALSE
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80 }else if(options$labels== "columns"){
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81 labs = labRow=FALSE
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82 }else if(options$labels== "none"){
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83 labs = labRow=FALSE, labCol=FALSE
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84 } else{
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85 }
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86
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87
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88
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2
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89
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3
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90 pdf(file="Rplot.pdf")
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10
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91 colorscale
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3
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92 heatmap.2(linput,
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13
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93 clust, scale = options$scale,
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94 col=colfunc(50), trace="none", density.info = "none", labs, margins=c(8,2),
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8
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95 main = options$title, key.xlab= options$key, keysize=1)
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3
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96 dev.off()
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