Mercurial > repos > mingchen0919 > aurora_deseq2
changeset 30:231de8741468 draft
planemo upload commit 3b9f9d4505f063da01213caf39f548ea1605c2f3-dirty
author | mingchen0919 |
---|---|
date | Fri, 14 Sep 2018 16:35:24 -0400 |
parents | 24a7f1f92db7 |
children | 7e767425bb3d |
files | rmarkdown_report.Rmd |
diffstat | 1 files changed, 7 insertions(+), 7 deletions(-) [+] |
line wrap: on
line diff
--- a/rmarkdown_report.Rmd Fri Sep 14 14:03:11 2018 -0400 +++ b/rmarkdown_report.Rmd Fri Sep 14 16:35:24 2018 -0400 @@ -6,12 +6,9 @@ --- ```{r setup, include=FALSE, warning=FALSE, message=FALSE} -knitr::opts_chunk$set(error = TRUE, echo = FALSE) +knitr::opts_chunk$set(error = TRUE, echo = TRUE) ``` -```{r debug-chunk} -print(1:4) -``` ```{css echo=FALSE} # code chunks scrollable @@ -66,7 +63,7 @@ coldata = read.csv(opt$X_B, row.names = 1, header = TRUE)[colnames(count_data),,drop=FALSE] ``` -```{r echo=FALSE} +```{r} dds = DESeqDataSetFromMatrix(countData = count_data, colData = coldata, design = formula(opt$X_C)) @@ -82,7 +79,7 @@ ``` -```{r echo=FALSE} +```{r} # save all padj sorted res to tool output directory padj_sorted_res = res[order(res$padj), ] write.table(padj_sorted_res, @@ -92,9 +89,12 @@ # save significant genes to a file in tool output directory sig_res = res[(res$padj < opt$X_I) & !is.na(res$padj), ] sig_res_sorted = sig_res[order(sig_res$padj), ] +sig_res_sorted$feature_id = rownames(sig_res_sorted) +n_col = ncol(sig_res_sorted) +sig_res_sorted = sig_res_sorted[, c(n_col, 1:(n_col - 1))] write.table(sig_res_sorted, file = paste0(opt$X_d, '/padj-sorted-significant-genes.txt'), - quote = FALSE) + quote = FALSE, row.names = FALSE) ``` ## MA-plot