Mercurial > repos > matthias > samtools_slice_bam
annotate samtools_sort/macros.xml @ 0:a5ad77502456 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
| author | matthias | 
|---|---|
| date | Fri, 22 Feb 2019 15:55:07 -0500 | 
| parents | |
| children | 
| rev | line source | 
|---|---|
| 
0
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
1 <macros> | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
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diff
changeset
 | 
2 <xml name="requirements"> | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
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diff
changeset
 | 
3 <requirements> | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
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changeset
 | 
4 <requirement type="package" version="@TOOL_VERSION@">samtools</requirement> | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
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changeset
 | 
5 <yield/> | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
6 </requirements> | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
7 </xml> | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
8 <token name="@TOOL_VERSION@">1.9</token> | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
9 <token name="@FLAGS@">#set $flags = sum(map(int, str($filter).split(',')))</token> | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
10 <token name="@PREPARE_IDX@"><![CDATA[ | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
11 ##prepare input and indices | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
12 ln -s '$input' infile && | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
13 #if $input.is_of_type('bam'): | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
14 #if str( $input.metadata.bam_index ) != "None": | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
15 ln -s '${input.metadata.bam_index}' infile.bai && | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
16 #else: | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
17 samtools index infile infile.bai && | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
18 #end if | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
19 #elif $input.is_of_type('cram'): | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
20 #if str( $input.metadata.cram_index ) != "None": | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
21 ln -s '${input.metadata.cram_index}' infile.crai && | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
22 #else: | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
23 samtools index infile infile.crai && | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
24 #end if | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
25 #end if | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
26 ]]></token> | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
27 <token name="@PREPARE_IDX_MULTIPLE@"><![CDATA[ | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
28 ##prepare input and indices | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
29 #for $i, $bam in enumerate( $input_bams ): | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
30 ln -s '$bam' '${i}' && | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
31 #if $bam.is_of_type('bam'): | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
32 #if str( $bam.metadata.bam_index ) != "None": | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
33 ln -s '${bam.metadata.bam_index}' '${i}.bai' && | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
34 #else: | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
35 samtools index '${i}' '${i}.bai' && | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
36 #end if | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
37 #elif $bam.is_of_type('cram'): | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
38 #if str( $bam.metadata.cram_index ) != "None": | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
39 ln -s '${bam.metadata.cram_index}' '${i}.crai' && | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
40 #else: | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
41 samtools index '${i}' '${i}.crai' && | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
42 #end if | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
43 #end if | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
44 #end for | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
45 ]]></token> | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
46 <token name="@PREPARE_FASTA_IDX@"><![CDATA[ | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
47 ##checks for reference data ($addref_cond.addref_select=="history" or =="cached") | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
48 ##and sets the -t/-T parameters accordingly: | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
49 ##- in case of history a symbolic link is used because samtools (view) will generate | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
50 ## the index which might not be possible in the directory containing the fasta file | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
51 ##- in case of cached the absolute path is used which allows to read the cram file | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
52 ## without specifying the reference | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
53 #if $addref_cond.addref_select == "history": | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
54 ln -s '${addref_cond.ref}' reference.fa && | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
55 samtools faidx reference.fa && | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
56 #set reffa="reference.fa" | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
57 #set reffai="reference.fa.fai" | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
58 #elif $addref_cond.addref_select == "cached": | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
59 #set reffa=str($addref_cond.ref.fields.path) | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
60 #set reffai=str($addref_cond.ref.fields.path)+".fai" | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
61 #else | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
62 #set reffa=None | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
63 #set reffai=None | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
64 #end if | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
65 ]]></token> | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
66 <token name="@ADDTHREADS@"><![CDATA[ | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
67 ##compute the number of ADDITIONAL threads to be used by samtools (-@) | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
68 addthreads=\${GALAXY_SLOTS:-1} && (( addthreads-- )) && | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
69 ]]></token> | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
70 <token name="@ADDMEMORY@"><![CDATA[ | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
71 ##compute the number of memory available to samtools sort (-m) | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
72 ##use only 75% of available: https://github.com/samtools/samtools/issues/831 | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
parents:  
diff
changeset
 | 
73 addmemory=\${GALAXY_MEMORY_MB_PER_SLOT:-768} && | 
| 
 
a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
matthias 
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74 ((addmemory=addmemory*75/100)) && | 
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75 ]]></token> | 
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76 <xml name="seed_input"> | 
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
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77 <param name="seed" type="integer" optional="True" label="Seed for random number generator" help="If empty a random seed is used." /> | 
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78 </xml> | 
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79 <xml name="flag_options"> | 
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
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80 <option value="1">read is paired</option> | 
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
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81 <option value="2">read is mapped in a proper pair</option> | 
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
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82 <option value="4">read is unmapped</option> | 
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a5ad77502456
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
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83 <option value="8">mate is unmapped</option> | 
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
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84 <option value="16">read reverse strand</option> | 
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
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85 <option value="32">mate reverse strand</option> | 
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
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86 <option value="64">read is the first in a pair</option> | 
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87 <option value="128">read is the second in a pair</option> | 
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88 <option value="256">alignment or read is not primary</option> | 
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89 <option value="512">read fails platform/vendor quality checks</option> | 
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90 <option value="1024">read is a PCR or optical duplicate</option> | 
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools commit 17bfa0739bf7c72cf92de0d7013437dff2bbba7f-dirty
 
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91 <option value="2048">supplementary alignment</option> | 
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92 </xml> | 
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93 | 
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94 <!-- region specification macros and tokens for tools that allow the specification | 
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95 of region by bed file / space separated list of regions --> | 
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96 <token name="@REGIONS_FILE@"><![CDATA[ | 
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97 #if $cond_region.select_region == 'tab': | 
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98 -t '$cond_region.targetregions' | 
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99 #end if | 
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100 ]]></token> | 
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101 <token name="@REGIONS_MANUAL@"><![CDATA[ | 
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102 #if $cond_region.select_region == 'text': | 
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103 #for $i, $x in enumerate($cond_region.regions_repeat): | 
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104 '${x.region}' | 
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105 #end for | 
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106 #end if | 
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107 ]]></token> | 
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108 <xml name="regions_macro"> | 
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109 <conditional name="cond_region"> | 
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110 <param name="select_region" type="select" label="Filter by regions" help="restricts output to only those alignments which overlap the specified region(s)"> | 
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111 <option value="no" selected="True">No</option> | 
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112 <option value="text">Manualy specify regions</option> | 
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113 <option value="tab">Regions from tabular file</option> | 
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114 </param> | 
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115 <when value="no"/> | 
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116 <when value="text"> | 
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117 <repeat name="regions_repeat" min="1" default="1" title="Regions"> | 
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118 <param name="region" type="text" label="region" help="format chr:from-to"> | 
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119 <validator type="regex" message="Required format: CHR[:FROM[-TO]]; where CHR: string containing any character except quotes, whitespace and colon; FROM and TO: any integer">^[^\s'\":]+(:\d+(-\d+){0,1}){0,1}$</validator> | 
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120 </param> | 
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121 </repeat> | 
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122 </when> | 
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123 <when value="tab"> | 
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124 <param name="targetregions" argument="-t/--target-regions" type="data" format="tabular" label="Target regions file" help="Do stats in these regions only. Tab-delimited file chr,from,to (1-based, inclusive)" /> | 
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125 </when> | 
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126 </conditional> | 
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127 </xml> | 
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128 | 
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129 <xml name="citations"> | 
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130 <citations> | 
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131 <citation type="bibtex"> | 
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132 @misc{SAM_def, | 
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133 title={Definition of SAM/BAM format}, | 
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134 url = {https://samtools.github.io/hts-specs/},} | 
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135 </citation> | 
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136 <citation type="doi">10.1093/bioinformatics/btp352</citation> | 
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137 <citation type="doi">10.1093/bioinformatics/btr076</citation> | 
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138 <citation type="doi">10.1093/bioinformatics/btr509</citation> | 
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139 <citation type="bibtex"> | 
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140 @misc{Danecek_et_al, | 
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141 Author={Danecek, P., Schiffels, S., Durbin, R.}, | 
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142 title={Multiallelic calling model in bcftools (-m)}, | 
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143 url = {http://samtools.github.io/bcftools/call-m.pdf},} | 
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144 </citation> | 
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145 <citation type="bibtex"> | 
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146 @misc{Durbin_VCQC, | 
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147 Author={Durbin, R.}, | 
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148 title={Segregation based metric for variant call QC}, | 
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149 url = {http://samtools.github.io/bcftools/rd-SegBias.pdf},} | 
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150 </citation> | 
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151 <citation type="bibtex"> | 
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152 @misc{Li_SamMath, | 
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153 Author={Li, H.}, | 
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154 title={Mathematical Notes on SAMtools Algorithms}, | 
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155 url = {http://www.broadinstitute.org/gatk/media/docs/Samtools.pdf},} | 
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156 </citation> | 
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157 <citation type="bibtex"> | 
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158 @misc{SamTools_github, | 
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159 title={SAMTools GitHub page}, | 
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160 url = {https://github.com/samtools/samtools},} | 
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161 </citation> | 
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162 </citations> | 
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163 </xml> | 
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164 <xml name="version_command"> | 
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165 <version_command><![CDATA[samtools 2>&1 | grep Version]]></version_command> | 
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166 </xml> | 
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167 <xml name="stdio"> | 
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168 <stdio> | 
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169 <exit_code range="1:" level="fatal" description="Error" /> | 
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170 </stdio> | 
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171 </xml> | 
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172 </macros> | 
