annotate dada2_seqCounts.xml @ 9:97fcf245fe07 draft

planemo upload for repository https://github.com/bernt-matthias/mb-galaxy-tools/tree/topic/dada2/tools/dada2 commit a54770771e567c7ad8a9dd75cc4689c3935ef11c
author matthias
date Tue, 28 May 2019 12:18:50 -0400
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children f71baf3a839b
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1 <tool id="dada2_seqCounts" name="dada2: sequence counts" version="@DADA2_VERSION@+galaxy@WRAPPER_VERSION@">
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2 <description></description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="requirements"/>
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7 <expand macro="version_command"/>
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8 <command detect_errors="exit_code"><![CDATA[
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9 Rscript '$dada2_script'
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10 ]]></command>
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11 <configfiles>
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12 <configfile name="dada2_script"><![CDATA[
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13 @READ_FOO@
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14 library(dada2, quietly=T)
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15
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16 getN <- function(x){ sum(getUniques(x)) }
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17
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18 df <- NULL
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19 #for $i, $rep in enumerate($inrep)
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20 samples = list()
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21 #for $s in $rep.input:
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22 ## for collection input assume identifiers are sample names
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23 #if $s.ext in ["tabular", "dada2_dada", "dada2_derep", "dada2_mergepairs"]
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24 sample_name <- '$s.element_identifier'
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25 samples[[sample_name]] <- $read_data( $s )
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26 #else
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27 samples <- $read_data( $s )
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28 #end if
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29 #if $s.ext == "tabular"
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30 tabular <- T
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31 #else
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32 tabular <- F
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33 #end if
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34 #end for
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35
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36 #if str($rep.name) == ""
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37 dname <- '$str(i)'
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38 #else
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39 dname <- '$rep.name'
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40 #end if
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41
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42 if( tabular ){
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43 tdf <- NULL
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44 for( n in names( samples ) ){
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45 if(is.null(tdf)){
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46 tdf <- samples[[n]]
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47 }else{
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48 tdf <- rbind(tdf, samples[[n]])
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49 }
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50 }
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51 names(tdf) <- paste( dname, names(tdf) )
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52 tdf <- cbind( data.frame(samples=names( samples )), tdf)
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53 }else{
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54 if(is.null(names(samples))){
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55 tdf <- data.frame( samples = row.names(samples) )
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56 }else{
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57 tdf <- data.frame( samples = names(samples) )
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58 }
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59 t <- tryCatch({
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60 sapply(samples, getN)
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61 },
0
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62 error=function(cond) {
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63 rowSums(samples)
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64 })
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65 tdf[[ dname ]] <- t
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66 }
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67 if(is.null(df)){
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68 df <- tdf
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69 }else{
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70 df <- merge( df, tdf, by="samples", all=T, no.dups=T)
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71 }
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72 #end for
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73 write.table(df, "$counts", quote=F, sep="\t", row.names = F, col.names = T)
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74 ]]></configfile>
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75 </configfiles>
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76 <inputs>
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77 <repeat name="inrep" title="data sets" min="1">
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78 <param name="input" type="data" multiple="true" format="tabular,@DADA_UNIQUES@,dada2_sequencetable,dada2_uniques" label="Dataset(s)"/>
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79 <param name="name" type="text" value="" optional="true" label="name"/>
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80 </repeat>
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81 </inputs>
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82 <outputs>
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83 <data name="counts" format="tabular" label="${tool.name} on ${on_string}"/>
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84 </outputs>
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85 <tests>
5
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86 <!-- test for the separate inputs (didn't implement as single test using the repeat
9
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87 since the sample name won't match anyway [galaxy does not allow to specify the
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88 names of the elements of the input collection for <param ... multiple="true">]) -->
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89 <test>
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90 <repeat name="inrep">
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91 <param name="input" value="filterAndTrim_F3D0.tab" ftype="tabular"/>
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92 <param name="name" value="filter"/>
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93 </repeat>
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94 <output name="counts" value="seqCounts_F3D0_filter.tab" ftype="tabular" />
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95 </test>
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96 <test>
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97 <repeat name="inrep">
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98 <param name="input" value="derepFastq_F3D0_R1.Rdata" ftype="dada2_derep"/>
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99 <param name="name" value="derepF"/>
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100 </repeat>
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101 <output name="counts" value="seqCounts_F3D0_derepF.tab" ftype="tabular" />
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102 </test>
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103 <test>
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104 <repeat name="inrep">
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105 <param name="input" value="dada_F3D0_R1.Rdata" ftype="dada2_dada"/>
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106 <param name="name" value="dadaF"/>
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107 </repeat>
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108 <output name="counts" value="seqCounts_F3D0_dadaF.tab" ftype="tabular" />
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109 </test>
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110 <test>
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111 <repeat name="inrep">
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112 <param name="input" value="mergePairs_F3D0.Rdata" ftype="dada2_mergepairs"/>
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113 <param name="name" value="merge"/>
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114 </repeat>
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115 <output name="counts" value="seqCounts_F3D0_merge.tab" ftype="tabular" />
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116 </test>
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117 <test>
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118 <repeat name="inrep">
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119 <param name="input" value="makeSequenceTable_F3D0.tab" ftype="dada2_sequencetable"/>
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120 <param name="name" value="seqtab"/>
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121 </repeat>
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122 <output name="counts" value="seqCounts_F3D0_seqtab.tab" ftype="tabular" />
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123 </test>
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124 <test>
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125 <repeat name="inrep">
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126 <param name="input" value="removeBimeraDenovo_F3D0.tab" ftype="dada2_sequencetable"/>
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127 <param name="name" value="nochim"/>
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128 </repeat>
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129 <output name="counts" value="seqCounts_F3D0_nochim.tab" ftype="tabular" />
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130 </test>
0
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131 </tests>
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132 <help><![CDATA[
3
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133 Description
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134 ...........
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135
0
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136 Get the counts of sequences per sample for the different stages of the dada pipeline.
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137
3
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138 Usage
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139 .....
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140
0
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141 **Inputs:**
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142
9
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143 Any number of results of dada2 steps in the following form:
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144 - a collection of results from derep, dada, or mergePairs; or the collection of statistics from filterAndTrim (the identifiers of the collection elements are used as sample names)
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145 - the result of makeSequenceTable or removeBimeraDenovo
0
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146
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147 **Output:**
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148
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149 A table containing the number of sequences per sample (rows) for each input (columns)
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150
3
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151 Details
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152 .......
0
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153
3
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154 For results from
0
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155
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156 - derepFastq, dada, and mergePairs the sum of the result of dada2's getUniques function is used
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157 - makeSequenceTable, and removeBimeraDenovo R's rowSums function is used
3
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158
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159 @HELP_OVERVIEW@
0
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160 ]]></help>
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161 <expand macro="citations"/>
11993afc394e planemo upload for repository https://github.com/bernt-matthias/mb-galaxy-tools/tree/topic/dada2/tools/dada2 commit d63c84012410608b3b5d23e130f0beff475ce1f8-dirty
matthias
parents:
diff changeset
162 </tool>