Mercurial > repos > jjohnson > gmap
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| author | jjohnson |
|---|---|
| date | Fri, 05 Oct 2012 13:51:49 -0400 |
| parents | |
| children | 74391fc6e3f2 |
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<tool id="gsnap" name="GSNAP" version="2.0.1"> <description>Genomic Short-read Nucleotide Alignment Program</description> <requirements> <requirement type="binary">gsnap</requirement> </requirements> <version_string>gsnap --version</version_string> <command> #import os.path, re gsnap --nthreads="4" --ordered #if $refGenomeSource.genomeSource == "gmapdb": #set $gmapdb = $os.listdir($refGenomeSource.gmapdb.extra_files_path)[0] --dir=$refGenomeSource.gmapdb.extra_files_path --db=$refGenomeSource.gmapdb.metadata.db_name #else: --dir=$os.path.dirname($refGenomeSource.gmapindex.value) --db=$os.path.basename($refGenomeSource.gmapindex.value) #end if #if $refGenomeSource.kmer != None and len($refGenomeSource.kmer.__str__) == 2: --kmer=$refGenomeSource.kmer #end if #if $refGenomeSource.use_splicing.src == 'gmapdb': #if $refGenomeSource.use_splicing.splicemap != None and len($refGenomeSource.use_splicing.splicemap.__str__) > 0: -s $refGenomeSource.use_splicing.splicemap.value #if $computation.trim_mismatch_score.__str__ == '0': $ambig_splice_noclip #end if #end if #elif $refGenomeSource.use_splicing.src == 'history': #if $refGenomeSource.use_splicing.splicemap != None and len($refGenomeSource.use_splicing.splicemap.__str__) > 0: -S $os.path.dirname($refGenomeSource.use_splicing.splicemap) -s $os.path.basename($refGenomeSource.use_splicing.splicemap) #if $computation.trim_mismatch_score.__str__ == '0': $ambig_splice_noclip #end if #end if #end if #if $refGenomeSource.use_snps.src == 'gmapdb': #if $refGenomeSource.use_snps.snpindex != None and len($refGenomeSource.use_snps.snpindex.__str__) > 0: -v $refGenomeSource.use_snps.snpindex.value #end if #elif $refGenomeSource.use_snps.src == 'history': #if $refGenomeSource.use_snps.snpindex != None and len($refGenomeSource.use_snps.snpindex.__str__) > 0: -V $refGenomeSource.use_snps.snpindex.extra_files_path -v $refGenomeSource.use_snps.snpindex.metadata.snps_name #end if #end if #if $refGenomeSource.mode.__str__ != '': --mode=$refGenomeSource.mode #end if #* ## No longer in options as of version 2011-11-30 #if $mapq_unique_score.__str__ != '': --mapq-unique-score=$mapq_unique_score #end if *# #if $computation.options == "advanced": #if $computation.max_mismatches.__str__ != '': --max-mismatches=$computation.max_mismatches #end if $computation.query_unk_mismatch $computation.genome_unk_mismatch #if $computation.terminal_threshold.__str__ != '': --terminal-threshold=$computation.terminal_threshold #end if #if $computation.indel_penalty.__str__ != '': --indel-penalty=$computation.indel_penalty #end if #if $computation.indel_endlength.__str__ != '': --indel-endlength=$computation.indel_endlength #end if #if $computation.max_middle_insertions.__str__ != '': --max-middle-insertions=$computation.max_middle_insertions #end if #if $computation.max_middle_deletions.__str__ != '': --max-middle-deletions=$computation.max_middle_deletions #end if #if $computation.max_end_insertions.__str__ != '': --max-end-insertions=$computation.max_end_insertions #end if #if $computation.max_end_deletions.__str__ != '': --max-end-deletions=$computation.max_end_deletions #end if #if $computation.suboptimal_levels.__str__ != '': --suboptimal-levels=$computation.suboptimal_levels #end if #if $computation.adapter_strip.__str__ != '': --adapter-strip=$computation.adapter_strip #end if #if $computation.trim_mismatch_score.__str__ != '': --trim-mismatch-score=$computation.trim_mismatch_score #end if #if $computation.trim_indel_score.__str__ != '': --trim-indel-score=$computation.trim_indel_score #end if ## TODO - do we need these options (Is it tally XOR runlength?): ## --tallydir= --use-tally=tally ## --runlengthdir --use-runlength=runlength #if $computation.use_tally != None and len($computation.use_tally.__str__) > 0: ##--tallydir $os.path.dirname($computation.use_tally) --use-tally $os.path.basename($computation.use_tally) --use-tally=$computation.use_tally #end if ## gmap options #if $computation.gmap_mode.__str__ != '' and $computation.gmap_mode.__str__ != 'None': --gmap-mode='$computation.gmap_mode' #end if #if $computation.trigger_score_for_gmap.__str__ != '': --trigger-score-for-gmap=$computation.trigger_score_for_gmap #end if #if $computation.max_gmap_pairsearch.__str__ != '' and $re.search("pairsearch",$computation.gmap_mode): --max-gmap-pairsearch=$computation.max_gmap_pairsearch #end if #if $computation.max_gmap_terminal.__str__ != '' and $re.search("terminal",$computation.gmap_mode): --max-gmap-terminal=$computation.max_gmap_terminal #end if #if $computation.max_gmap_improvement.__str__ != '' and $re.search("improv",$computation.gmap_mode): --max-gmap-improvement=$computation.max_gmap_improvement #end if #if $computation.microexon_spliceprob.__str__ != '': --microexon-spliceprob=$computation.microexon_spliceprob #end if #end if #if $splicing.options == "advanced": $splicing.novelsplicing #if $splicing.localsplicedist.__str__ != '': --localsplicedist=$splicing.localsplicedist #end if #if $splicing.local_splice_penalty.__str__ != '': --local-splice-penalty=$splicing.local_splice_penalty #end if #if $splicing.distant_splice_penalty.__str__ != '': --distant-splice-penalty=$splicing.distant_splice_penalty #end if #if $splicing.local_splice_endlength.__str__ != '': --local-splice-endlength=$splicing.local_splice_endlength #end if #if $splicing.distant_splice_endlength.__str__ != '': --distant-splice-endlength=$splicing.distant_splice_endlength #end if #if $splicing.distant_splice_identity.__str__ != '': --distant-splice-identity=$splicing.distant_splice_identity #end if #end if #if $output.options == "advanced": #if $output.npath.__str__ != '': --npath=$output.npath #end if $output.quiet_if_excessive $output.show_refdiff $output.clip_overlap #end if #if $result.format == "sam": --format=sam $result.no_sam_headers #if $result.read_group_id.__str__.strip != '': --read-group-id='$result.read_group_id' #end if #if $result.read_group_name.__str__ != '': --read-group-name='$result.read_group_name' #end if #if $result.read_group_library.__str__ != '': --read-group-library='$result.read_group_library' #end if #if $result.read_group_platform.__str__ != '': --read-group-platform='$result.read_group_platform' #end if #if $result.quality_shift.__str__ != '': --quality-shift=$result.quality_shift #end if #elif $result.format == "goby": #if $result.goby_output.__str__ != '': --goby-output='$result.goby_output' #end if #if $result.creads_window_start.__str__ != '': --creads-window-start=$result.creads_window_start #end if #if $result.creads_window_end.__str__ != '': --creads-window-end=$result.creads_window_end #end if $result.creads_complement #end if #if $results.split_output == 'yes': --split-output=gsnap_out #if $results.fails.choice == 'nofails': --nofails #elif $results.fails.choice == 'failsonly': --failsonly #end if $results.fails_as_input #else #if $results.fails.choice == 'nofails': --nofails #elif $results.fails.choice == 'failsonly': --failsonly $results.fails.fails_as_input #end if #end if #if $seq.format == "gsnap_fasta": $seq.circularinput $seq.gsnap #else if $seq.format == "fastq": #if $seq.barcode_length.__str__ != '': --barcode-length=$seq.barcode_length #end if #if $seq.fastq_id_start.__str__ != '': --fastq-id-start=$seq.fastq_id_start #end if #if $seq.fastq_id_end.__str__ != '': --fastq-id-end=$seq.fastq_id_end #end if #if $seq.filter_chastity.__str__ != 'off': --filter-chastity=$seq.filter_chastity #end if #if $seq.paired.ispaired.__str__ == 'yes': #if $seq.paired.pairmax_dna.__str__ != '': --pairmax-dna=$seq.paired.pairmax_dna #end if #if $seq.paired.pairmax_rna.__str__ != '': --pairmax-rna=$seq.paired.pairmax_rna #end if #if $seq.paired.pairexpect.__str__ != '': --pairexpect=$seq.paired.pairexpect #end if #if $seq.paired.pairdev.__str__ != '': --pairdev=$seq.paired.pairdev #end if $seq.fastq $seq.paired.fastq #else $seq.fastq #end if #end if #if $results.split_output == 'yes': 2> $gsnap_stderr #else: #if $results.fails.choice.__str__ == 'failsonly' and $results.fails.fails_as_input.__str__ != '': 2> $gsnap_stderr > $gsnap_fq #else 2> $gsnap_stderr > $gsnap_out #end if #end if </command> <inputs> <!-- Input data --> <conditional name="seq"> <param name="format" type="select" label="<H2>Input Sequences</H2>Select the input format" help=""> <option value="fastq">Fastq</option> <!-- <option value="goby">Goby compact-reads</option> --> <option value="gsnap_fasta">GNSAP fasta</option> </param> <when value="fastq"> <param name="fastq" type="data" format="fastq" label="Select a fastq dataset" /> <conditional name="paired"> <param name="ispaired" type="boolean" truevalue="yes" falsevalue="no" checked="false" label="Use Paired Reads?"/> <when value="no"/> <when value="yes"> <param name="fastq" type="data" format="fastq" label="Select the paired reads reverse dataset" /> <param name="orientation" type="select" label="Orientation of paired-end reads" help=""> <option value="FR">fwd-rev, typical Illumina default</option> <option value="RF">rev-fwd, for circularized inserts</option> <option value="FF">fwd-fwd, same strand</option> </param> <param name="pairmax_dna" type="integer" value="" optional="true" label="Max total genomic length for DNA-Seq paired reads, or other reads without splicing (default 1000)." help="Used if no splice file is provided and novelsplicing is off."/> <param name="pairmax_rna" type="integer" value="" optional="true" label="Max total genomic length for RNA-Seq paired reads, or other reads that could have a splice (default 200000)." help="Used when novel splicing is specified or a splice file is provided. Should probably match the value for localsplicedist."/> <param name="pairexpect" type="integer" value="" optional="true" label="Expected paired-end length" help="Used for calling splices in medial part of paired-end reads (default 200)"/> <param name="pairdev" type="integer" value="" optional="true" label="Allowable deviation from expected paired-end length" help="Used for calling splices in medial part of paired-end reads (default 25)"/> </when> </conditional> <param name="barcode_length" type="integer" value="" optional="true" label="Amount of barcode to remove from start of read (default 0)" /> <param name="fastq_id_start" type="integer" value="" optional="true" label="Starting field of identifier in FASTQ header, whitespace-delimited, starting from 1" /> <param name="fastq_id_end" type="integer" value="" optional="true" label="Ending field of identifier in FASTQ header, whitespace-delimited, starting from 1" help="Examples: <br>@HWUSI-EAS100R:6:73:941:1973#0/1 <br> . start=1, end=1 (default) => identifier is HWUSI-EAS100R:6:73:941:1973#0/1 <br>@SRR001666.1 071112_SLXA-EAS1_s_7:5:1:817:345 length=36 <br> . start=1, end=1 => identifier is SRR001666.1 <br> . start=2, end=2 => identifier is 071112_SLXA-EAS1_s_7:5:1:817:345 <br> . start=1, end=2 => identifier is SRR001666.1 071112_SLXA-EAS1_s_7:5:1:817:345" /> <param name="filter_chastity" type="select" label="Skip reads marked by the Illumina chastity program" help="String after the accession having a 'Y' after the first colon, like this: <br>@accession 1:Y:0:CTTGTA <br>where the 'Y' signifies filtering by chastity. <br> For 'either', a 'Y' on either end of a paired-end read will be filtered. <br> For 'both', a 'Y' is required on both ends of a paired-end read (or on the only end of a single-end read)" > <option value="off">off - no filtering</option> <option value="either">either - a 'Y' on either end of a paired-end read</option> <option value="both">both - a 'Y' is required on both ends of a paired-end read or the only end of a single-end read</option> </param> </when> <!-- <when value="goby"> </when> --> <when value="gsnap_fasta"> <param name="gsnap" type="data" format="fasta" label="Select a single-end dataset" help="GSNAP fasta must have the sequence entirely on one line, a second line is interpreted as the paired-end sequence"/> <param name="circularinput" type="boolean" checked="false" truevalue="--circular-input=true" falsevalue="" label="Circular-end data (paired reads are on same strand)"/> </when> </conditional> <!-- No longer in options as of version 2011-11-30 <param name="mapq_unique_score" type="integer" value="" optional="true" label="MAPQ score threshold" help="For multiple results, consider as a unique result if only one of the results has a MAPQ score equal or greater than this (if not selected, then reports all multiple results, up to npaths)" /> --> <!-- GMAPDB for alignment --> <conditional name="refGenomeSource"> <param name="genomeSource" type="select" label="<HR><H2>Align To</H2>Will you select a reference genome from your history or use a built-in index?" help="Built-ins were indexed using default options"> <option value="indexed">Use a built-in index</option> <option value="gmapdb">Use a gmapdb from your history</option> </param> <when value="indexed"> <param name="gmapindex" type="select" label="Select a reference genome" help="if your genome of interest is not listed - contact Galaxy team"> <options from_file="gmap_indices.loc"> <column name="uid" index="0" /> <column name="dbkey" index="1" /> <column name="name" index="2" /> <column name="kmers" index="3" /> <column name="maps" index="4" /> <column name="snps" index="5" /> <column name="value" index="6" /> </options> </param> <param name="kmer" type="select" data_ref="gmapindex" label="kmer size" help="Defaults to highest available kmer size"> <options from_file="gmap_indices.loc"> <column name="name" index="3"/> <column name="value" index="3"/> <filter type="param_value" ref="gmapindex" column="6"/> <filter type="multiple_splitter" column="3" separator=","/> <filter type="add_value" name="" value=""/> <filter type="sort_by" column="3"/> </options> </param> <param name="mode" type="select" label="Alignment mode" help="Assumes cmetindex and atoiindex were run on the gmap datatbase."> <option value="">standard</option> <option value="cmet-stranded">cmet-stranded for bisulfite-treated DNA reads (tolerance to C-to-T changes)</option> <option value="cmet-nonstranded">cmet-nonstranded for bisulfite-treated DNA reads (tolerance to C-to-T changes)</option> <option value="atoi-stranded">atoi-stranded for RNA-editing tolerance (A-to-G changes)</option> <option value="atoi-nonstranded">atoi-nonstranded for RNA-editing tolerance (A-to-G changes)</option> </param> <conditional name="use_splicing"> <param name="src" type="select" label="<HR>Known Splicesite and Introns" help="Look for splicing involving known sites or known introns at short or long distances See README instructions for the distinction between known sites and known introns"> <option value="none" selected="true">None</option> <option value="gmapdb">From the GMAP Database</option> <option value="history">A Map in your history</option> </param> <when value="none"/> <when value="history"> <param name="splicemap" type="data" format="splicesites.iit,introns.iit" metadata_name="dbkey" label="Select a splicesite map" help="built with GMAP IIT"/> </when> <when value="gmapdb"> <param name="splicemap" type="select" data_ref="gmapindex" label="Use map for splicing involving known sites or known introns" help=""> <options from_file="gmap_indices.loc"> <column name="name" index="4"/> <column name="value" index="4"/> <filter type="param_value" ref="gmapindex" column="6"/> <filter type="multiple_splitter" column="4" separator=","/> <filter type="add_value" name="" value=""/> <filter type="sort_by" column="4"/> </options> </param> </when> </conditional> <conditional name="use_snps"> <param name="src" type="select" label="<HR>Known SNPs" help="for SNP tolerant alignments"> <option value="none" selected="true">None</option> <option value="gmapdb">From the GMAP Database</option> <option value="history">A SNP Index in your history</option> </param> <when value="none"/> <when value="history"> <param name="snpindex" type="data" format="gmapsnpindex" metadata_name="dbkey" label="Select a snpindex" help="built with GMAP SNP Index"/> </when> <when value="gmapdb"> <param name="snpindex" type="select" data_ref="gmapindex" label="Use database containing known SNPs" help=""> <options from_file="gmap_indices.loc"> <column name="name" index="5"/> <column name="value" index="5"/> <filter type="param_value" ref="gmapindex" column="6"/> <filter type="multiple_splitter" column="5" separator=","/> <filter type="add_value" name="" value=""/> <filter type="sort_by" column="5"/> </options> </param> </when> </conditional> </when> <when value="gmapdb"> <param name="gmapdb" type="data" format="gmapdb" metadata_name="dbkey" label="Select a gmapdb" help="A GMAP database built with GMAP Build"/> <param name="kmer" type="select" data_ref="gmapdb" label="kmer size" help="Defaults to highest available kmer size"> <options> <filter type="data_meta" ref="gmapdb" key="kmers" multiple="True" separator=","/> </options> </param> <param name="mode" type="select" label="Alignment mode" help="Assumes cmetindex and atoiindex were run on the gmap datatbase."> <option value="">standard</option> <option value="cmet-stranded">cmet-stranded for bisulfite-treated DNA reads (tolerance to C-to-T changes)</option> <option value="cmet-nonstranded">cmet-nonstranded for bisulfite-treated DNA reads (tolerance to C-to-T changes)</option> <option value="atoi-stranded">atoi-stranded for RNA-editing tolerance (A-to-G changes)</option> <option value="atoi-nonstranded">atoi-nonstranded for RNA-editing tolerance (A-to-G changes)</option> </param> <conditional name="use_splicing"> <param name="src" type="select" label="<HR>Known Splicesite and Introns" help="Look for splicing involving known sites or known introns at short or long distances See README instructions for the distinction between known sites and known introns"> <option value="none" selected="true">None</option> <option value="gmapdb">From the GMAP Database</option> <option value="history">A Map in your history</option> </param> <when value="none"/> <when value="history"> <param name="splicemap" type="data" format="splicesites.iit,introns.iit" metadata_name="dbkey" label="Select a splicesite map" help="built with GMAP IIT"/> <param name="ambig_splice_noclip" type="boolean" checked="false" truevalue="--ambig-splice-noclip" falsevalue="" label="Do not clip at ambiguous splice sites" help="For ambiguous known splicing at ends of the read, do not clip at the splice site, but extend instead into the intron. This flag makes sense only if you are trying to eliminate all soft clipping with --trim-mismatch-score=0"/> </when> <when value="gmapdb"> <param name="splicemap" type="select" data_ref="gmapdb" label="Use map for splicing involving known sites or known introns" help=""> <options> <filter type="data_meta" ref="gmapdb" key="maps" multiple="True"/> </options> </param> <param name="ambig_splice_noclip" type="boolean" checked="false" truevalue="--ambig-splice-noclip" falsevalue="" label="Do not clip at ambiguous splice sites" help="For ambiguous known splicing at ends of the read, do not clip at the splice site, but extend instead into the intron. This flag makes sense only if you are trying to eliminate all soft clipping with --trim-mismatch-score=0"/> </when> </conditional> <conditional name="use_snps"> <param name="src" type="select" label="<HR>Known SNPs" help="for SNP tolerant alignments"> <option value="none" selected="true">None</option> <option value="gmapdb">From the GMAP Database</option> <option value="history">A SNP Index in your history</option> </param> <when value="none"/> <when value="history"> <param name="snpindex" type="data" format="gmapsnpindex" metadata_name="dbkey" label="Select a snpindex" help="built with GMAP SNP Index"/> </when> <when value="gmapdb"> <param name="snpindex" type="select" data_ref="gmapdb" label="Use database containing known SNPs" help=""> <options> <filter type="data_meta" ref="gmapdb" key="snps" multiple="True" separator=","/> </options> </param> </when> </conditional> </when> </conditional> <!-- Computation options --> <conditional name="computation"> <param name="options" type="select" label="<HR>Computational Settings" help=""> <option value="default">Use default settings</option> <option value="advanced">Set Computation Options</option> </param> <when value="default"/> <when value="advanced"> <param name="max_mismatches" type="float" value="" optional="true" label="Maximum number of mismatches allowed (uses default when negative)" help="Defaults to the ultrafast level of ((readlength+2)/12 - 2)). If specified between 0.0 and 1.0, then treated as a fraction of each read length. Otherwise, treated as an integral number of mismatches (including indel and splicing penalties) For RNA-Seq, you may need to increase this value slightly to align reads extending past the ends of an exon."> <validator type="in_range" message="The mismatches must >= 0." min="0."/> </param> <param name="query_unk_mismatch" type="boolean" checked="false" truevalue="--query-unk-mismatch=1" falsevalue="" label="Count unknown (N) characters in the query as a mismatch"/> <param name="genome_unk_mismatch" type="boolean" checked="true" truevalue="" falsevalue="--genome-unk-mismatch=0" label="Count unknown (N) characters in the genome as a mismatch"/> <param name="terminal_threshold" type="integer" value="" optional="true" label="Threshold for searching for a terminal alignment (default 2)" help="(from one end of the read to the best possible position at the other end). For example, if this value is 2, then if GSNAP finds an exact or 1-mismatch alignment, it will not try to find a terminal alignment. Note that this default value may not be low enough if you want to obtain terminal alignments for very short reads, although such reads probably don't have enough specificity for terminal alignments anyway." /> <param name="indel_penalty" type="integer" value="" optional="true" label="Penalty for an indel (default 2)" help="Counts against mismatches allowed. To find indels, make indel-penalty less than or equal to max-mismatches. A value < 2 can lead to false positives at read ends" /> <param name="indel_endlength" type="integer" value="" optional="true" label="Minimum length at end required for indel alignments (default 4)" /> <param name="max_middle_insertions" type="integer" value="" optional="true" label="Maximum number of middle insertions allowed (default 9)" /> <param name="max_middle_deletions" type="integer" value="" optional="true" label="Maximum number of middle deletions allowed (default 30)" /> <param name="max_end_insertions" type="integer" value="" optional="true" label="Maximum number of end insertions allowed (default 3)" /> <param name="max_end_deletions" type="integer" value="" optional="true" label="Maximum number of end deletions allowed (default 6)" /> <param name="suboptimal_levels" type="integer" value="" optional="true" label="Report suboptimal hits beyond best hit (default 0)" help="All hits with best score plus suboptimal-levels are reported" /> <param name="adapter_strip" type="select" label="Method for removing adapters from reads" help="paired removes adapters from paired-end reads if a concordant or paired alignment cannot be found from the original read"> <option value="paired" selected="true">paired</option> <option value="off">off</option> </param> <param name="trim_mismatch_score" type="integer" value="" optional="true" label="Score to use for mismatches when trimming at ends (default is -3)" help="to turn off trimming, specify 0 (Warning: turning trimming off will give false positive mismatches at the ends of reads)"/> <param name="trim_indel_score" type="integer" value="" optional="true" label="Score to use for indels when trimming at ends (default is -4)" help="to turn off trimming, specify 0 (Warning: turning trimming off will give false positive indels at the ends of reads)"/> <param name="use_tally" type="data" format="tally.iit" optional="true" metadata_name="dbkey" label="Select a tally IIT file to resolve concordant multiple results" help="generated by gsnap_tally and iit_store"/> <!-- tallydir=STRING Directory for tally IIT file to resolve concordant multiple results (default is location of genome index files specified using -D and -d). Note: can just give full path name to use-tally instead. use-tally=STRING Use this tally IIT file to resolve concordant multiple results runlengthdir=STRING Directory for runlength IIT file to resolve concordant multiple results (default is location of genome index files specified using -D and -d). Note: can just give full path name to use-runlength instead. use-runlength=STRING Use this runlength IIT file to resolve concordant multiple results --> <!-- Options for GMAP alignment within GSNAP --> <param name="gmap_mode" type="select" multiple="true" optional="true" display="checkboxes" label="Cases to use GMAP for complex alignments containing multiple splices or indels" help="Default: pairsearch,terminal,improve"> <option value="pairsearch" selected="true">pairsearch</option> <option value="terminal" selected="true">terminal</option> <option value="improve" selected="true">improve</option> </param> <param name="trigger_score_for_gmap" type="integer" value="" optional="true" label="GMAP pairsearch threshold (default 5)" help="Try GMAP pairsearch on nearby genomic regions if best score (the total of both ends if paired-end) exceeds this value (default 5)" /> <param name="max_gmap_pairsearch" type="integer" value="" optional="true" label="GMAP pairsearch threshold (default 3)" help="Perform GMAP pairsearch on nearby genomic regions up to this many candidate ends (default 3)." /> <param name="max_gmap_terminal" type="integer" value="" optional="true" label="GMAP terminal threshold (default 3)" help="Perform GMAP terminal on nearby genomic regions up to this many candidate ends (default 3)." /> <param name="max_gmap_improvement" type="integer" value="" optional="true" label="GMAP improvement threshold (default 3)" help="Perform GMAP improvement on nearby genomic regions up to this many candidate ends (default 3)." /> <param name="microexon_spliceprob" type="float" value="" optional="true" label="GMAP microexons threshold (default .90)" help="Allow microexons only if one of the splice site probabilities is greater than this value." > <validator type="in_range" message="The microexons probability must be between 0. and 1." min="0." max="1."/> </param> </when> </conditional> <conditional name="splicing"> <param name="options" type="select" label="<HR>Splicing options for RNA-Seq" help=""> <option value="default">Use default settings</option> <option value="advanced">Set Splicing Options</option> </param> <when value="default"/> <when value="advanced"> <!-- Splicing options for RNA-Seq --> <!-- use-splicing This should be either a select list from the gmapdb maps or a data type using splicesdir and use-splicing --> <!-- Neither novel splicing (-N) nor known splicing (-s) turned on => assume reads are DNA-Seq (genomic) --> <param name="novelsplicing" type="boolean" checked="false" truevalue="--novelsplicing=1" falsevalue="" label="Look for novel splicing "/> <param name="localsplicedist" type="integer" value="" optional="true" label="Definition of local novel splicing event (default 200000)"/> <param name="local_splice_penalty" type="integer" value="" optional="true" label="Penalty for a local splice (default 0). Counts against mismatches allowed"/> <param name="distant_splice_penalty" type="integer" value="" optional="true" label="Penalty for a distant splice (default 3). Counts against mismatches allowed" help="A distant splice is one where the intron length exceeds the value of localsplicedist or is an inversion, scramble, or translocation between two different chromosomes. Counts against mismatches allowed"/> <param name="distant_splice_endlength" type="integer" value="" optional="true" label="Minimum length at end required for distant spliced alignments" help="(default 16, min is the kmer length)"/> <param name="shortend_splice_endlength" type="integer" value="" optional="true" label="Minimum length at end required for short-end spliced alignments" help="(default 2, but unless known splice sites are provided, GSNAP may still need the end length to be the value of kmer size to find a given splice"/> <param name="distant_splice_identity" type="float" value="" optional="true" label="Minimum identity at end required for distant spliced alignments (default 0.95)"/> <param name="antistranded_penalty" type="integer" value="" optional="true" label="Penalty for antistranded splicing when using stranded RNA-Seq protocols" help="A positive value, such as 1, expects antisense on the first read and sense on the second read. Default is 0, which treats sense and antisense equally well"/> </when> </conditional> <!-- Output data --> <conditional name="output"> <param name="options" type="select" label="<HR><H2>Output</H2>Output options for RNA-Seq" help=""> <option value="default">Use default settings</option> <option value="advanced">Set Output Options</option> </param> <when value="default"/> <when value="advanced"> <param name="npath" type="integer" value="" optional="true" label="Maximum number of paths to print (default 100)"/> <param name="quiet_if_excessive" type="boolean" checked="false" truevalue="--quiet-if-excessive" falsevalue="" label="Quiet if Excessive" help="If more than maximum number of paths are found, then nothing is printed."/> <param name="show_refdiff" type="boolean" checked="false" truevalue="--show-refdiff" falsevalue="" label="Show SNP-tolerant alignment" help="For GSNAP output in SNP-tolerant alignment, shows all differences relative to the reference genome as lower case (otherwise, it shows all differences relative to both the reference and alternate genome)"/> <param name="clip_overlap" type="boolean" checked="false" truevalue="--clip-overlap" falsevalue="" label="Clip Overlap" help="For paired-end reads whose alignments overlap, clip the overlapping region."/> </when> </conditional> <conditional name="result"> <param name="format" type="select" label="Select the output format" help=""> <option value="sam">SAM</option> <!-- goby should only be an option if the input is in goby format <option value="goby">Goby</option> --> <option value="gsnap">GSNAP default output</option> </param> <when value="gsnap"> </when> <when value="sam"> <param name="no_sam_headers" type="boolean" truevalue="--no-sam-headers" falsevalue="" checked="false" label="Do not print headers beginning with '@'"/> <param name="read_group_id" type="text" value="" optional="true" label="Value to put into read-group id (RG-ID) field"/> <param name="read_group_name" type="text" value="" optional="true" label="Value to put into read-group name (RG-SM) field"/> <param name="read_group_library" type="text" value="" optional="true" label="Value to put into read-group library (RG-LB) field"/> <param name="read_group_platform" type="text" value="" optional="true" label="Value to put into read-group library platform (RG-PL) field"/> <param name="quality_shift" type="integer" value="" optional="true" label="Shift FASTQ quality scores by this amount in SAM output (default -31)"/> </when> <!-- <when value="goby"> <param name="goby_output" type="text" value="" label="Basename for Goby output files"/> <param name="creads_window_start" type="integer" value="" optional="true" label="Compact reads window start (default: 0=start of file)"/> <param name="creads_window_end" type="integer" value="" optional="true" label="Compact reads window end (default: 0=end of file)"/> <param name="creads_complement" type="boolean" truevalue="-\-creads-complement" falsevalue="" checked="false" label="Complement read sequences (without reversing)"/> </when> --> </conditional> <!-- TODO combine fails and split_output --> <conditional name="results"> <param name="split_output" type="select" label="<HR>Split outputs" help="Separate outputs for: nomapping, halfmapping_uniq, halfmapping_mult, unpaired_uniq, unpaired_mult, paired_uniq, paired_mult, concordant_uniq, and concordant_mult results"> <option value="no">no</option> <option value="yes">yes</option> </param> <when value="no"> <conditional name="fails"> <param name="choice" type="select" label="How to deal with fails" help=""> <option value="default">default - include them in results</option> <option value="nofails">nofails - exclude fails from results</option> <option value="failsonly">failsonly - only output failing results</option> </param> <when value="default"/> <when value="nofails"/> <when value="failsonly"> <param name="fails_as_input" type="boolean" truevalue="--fails-as-input" falsevalue="" checked="false" label="Print completely failed alignments as input FASTA or FASTQ format" help=""/> </when> </conditional> </when> <when value="yes"> <conditional name="fails"> <param name="choice" type="select" label="How to deal with fails" help=""> <option value="default">default - include them in results</option> <option value="nofails">nofails - exclude fails from results</option> <option value="failsonly">failsonly - only output failing results</option> </param> <when value="default"/> <when value="nofails"/> <when value="failsonly"/> </conditional> <param name="fails_as_input" type="boolean" truevalue="--fails-as-input" falsevalue="" checked="false" label="Print completely failed alignments as input FASTA or FASTQ format" help=""/> </when> </conditional> </inputs> <outputs> <data format="txt" name="gsnap_stderr" label="${tool.name} on ${on_string}: gsnap.log"/> <data format="txt" name="gsnap_out" label="${tool.name} on ${on_string} ${result.format}" > <filter>(results['split_output'] == 'no' and (results['fails']['choice'] != 'failsonly' or results['fails']['fails_as_input'] == False))</filter> <change_format> <when input="result['format']" value="sam" format="sam"/> <when input="result['format']" value="gsnap" format="gsnap"/> </change_format> </data> <data format="fastq" name="gsnap_fq" label="${tool.name} on ${on_string} fails.fq" > <filter>(results['split_output'] == 'no' and results['fails']['choice'] == 'failsonly' and results['fails']['fails_as_input'] == True)</filter> </data> <!-- nomapping, halfmapping_uniq, halfmapping_mult, unpaired_uniq, unpaired_mult, paired_uniq, paired_mult, concordant_uniq, concordant_mult --> <data format="txt" name="unpaired_mult" label="${tool.name} on ${on_string} unpaired_mult.${result.format}" from_work_dir="gsnap_out.unpaired_mult"> <filter>(results['split_output'] == 'yes')</filter> <change_format> <when input="result['format']" value="sam" format="sam"/> <when input="result['format']" value="gsnap" format="gsnap"/> </change_format> </data> <data format="txt" name="unpaired_uniq" label="${tool.name} on ${on_string} unpaired_uniq.${result.format}" from_work_dir="gsnap_out.unpaired_uniq"> <filter>(results['split_output'] == 'yes')</filter> <change_format> <when input="result['format']" value="sam" format="sam"/> <when input="result['format']" value="gsnap" format="gsnap"/> </change_format> </data> <data format="txt" name="unpaired_transloc" label="${tool.name} on ${on_string} unpaired_transloc.${result.format}" from_work_dir="gsnap_out.unpaired_transloc"> <filter>(results['split_output'] == 'yes')</filter> <change_format> <when input="result['format']" value="sam" format="sam"/> <when input="result['format']" value="gsnap" format="gsnap"/> </change_format> </data> <data format="txt" name="halfmapping_mult" label="${tool.name} on ${on_string} halfmapping_mult.${result.format}" from_work_dir="gsnap_out.halfmapping_mult"> <filter>(results['split_output'] == 'yes' and seq['format'] == 'fastq' and seq['paired']['ispaired'] == True)</filter> <change_format> <when input="result['format']" value="sam" format="sam"/> <when input="result['format']" value="gsnap" format="gsnap"/> </change_format> </data> <data format="txt" name="halfmapping_uniq" label="${tool.name} on ${on_string} halfmapping_uniq.${result.format}" from_work_dir="gsnap_out.halfmapping_uniq"> <filter>(results['split_output'] == 'yes' and seq['format'] == 'fastq' and seq['paired']['ispaired'] == True)</filter> <change_format> <when input="result['format']" value="sam" format="sam"/> <when input="result['format']" value="gsnap" format="gsnap"/> </change_format> </data> <data format="txt" name="halfmapping_transloc" label="${tool.name} on ${on_string} halfmapping_transloc.${result.format}" from_work_dir="gsnap_out.halfmapping_transloc"> <filter>(results['split_output'] == 'yes' and seq['format'] == 'fastq' and seq['paired']['ispaired'] == True)</filter> <change_format> <when input="result['format']" value="sam" format="sam"/> <when input="result['format']" value="gsnap" format="gsnap"/> </change_format> </data> <data format="txt" name="paired_mult" label="${tool.name} on ${on_string} paired_mult.${result.format}" from_work_dir="gsnap_out.paired_mult"> <filter>(results['split_output'] == 'yes' and seq['format'] == 'fastq' and seq['paired']['ispaired'] == True)</filter> <change_format> <when input="result['format']" value="sam" format="sam"/> <when input="result['format']" value="gsnap" format="gsnap"/> </change_format> </data> <data format="txt" name="paired_uniq" label="${tool.name} on ${on_string} paired_uniq.${result.format}" from_work_dir="gsnap_out.paired_uniq"> <filter>(results['split_output'] == 'yes' and seq['format'] == 'fastq' and seq['paired']['ispaired'] == True)</filter> <change_format> <when input="result['format']" value="sam" format="sam"/> <when input="result['format']" value="gsnap" format="gsnap"/> </change_format> </data> <data format="txt" name="paired_transloc" label="${tool.name} on ${on_string} paired_transloc.${result.format}" from_work_dir="gsnap_out.paired_transloc"> <filter>(results['split_output'] == 'yes' and seq['format'] == 'fastq' and seq['paired']['ispaired'] == True)</filter> <change_format> <when input="result['format']" value="sam" format="sam"/> <when input="result['format']" value="gsnap" format="gsnap"/> </change_format> </data> <data format="txt" name="concordant_mult" label="${tool.name} on ${on_string} concordant_mult.${result.format}" from_work_dir="gsnap_out.concordant_mult"> <filter>(results['split_output'] == 'yes' and seq['format'] == 'fastq' and seq['paired']['ispaired'] == True)</filter> <change_format> <when input="result['format']" value="sam" format="sam"/> <when input="result['format']" value="gsnap" format="gsnap"/> </change_format> </data> <data format="txt" name="concordant_uniq" label="${tool.name} on ${on_string} concordant_uniq.${result.format}" from_work_dir="gsnap_out.concordant_uniq"> <filter>(results['split_output'] == 'yes' and seq['format'] == 'fastq' and seq['paired']['ispaired'] == True)</filter> <change_format> <when input="result['format']" value="sam" format="sam"/> <when input="result['format']" value="gsnap" format="gsnap"/> </change_format> </data> <data format="txt" name="concordant_transloc" label="${tool.name} on ${on_string} concordant_transloc.${result.format}" from_work_dir="gsnap_out.concordant_transloc"> <filter>(results['split_output'] == 'yes' and seq['format'] == 'fastq' and seq['paired']['ispaired'] == True)</filter> <change_format> <when input="result['format']" value="sam" format="sam"/> <when input="result['format']" value="gsnap" format="gsnap"/> </change_format> </data> <data format="txt" name="nomapping" label="${tool.name} on ${on_string} nomapping.${result.format}" from_work_dir="gsnap_out.nomapping"> <filter>(results['split_output'] == 'yes' and results['fails_as_input'] == False)</filter> <change_format> <when input="result['format']" value="sam" format="sam"/> <when input="result['format']" value="gsnap" format="gsnap"/> </change_format> </data> <data format="fastq" name="nomapping_fq" label="${tool.name} on ${on_string} nomapping.fq" from_work_dir="gsnap_out.nomapping.fq"> <filter>(results['split_output'] == 'yes' and seq['format'] == 'fastq' and seq['paired']['ispaired'] == False)</filter> </data> <data format="fastq" name="nomapping_1_fq" label="${tool.name} on ${on_string} nomapping.1.fq" from_work_dir="gsnap_out.nomapping.1.fq"> <filter>(results['split_output'] == 'yes' and seq['format'] == 'fastq' and seq['paired']['ispaired'] == True)</filter> </data> <data format="fastq" name="nomapping_2_fq" label="${tool.name} on ${on_string} nomapping.2.fq" from_work_dir="gsnap_out.nomapping.2.fq"> <filter>(results['split_output'] == 'yes' and seq['format'] == 'fastq' and seq['paired']['ispaired'] == True)</filter> </data> <!-- Will problay need wrapper code to generate composite datatype for goby alignment <data format="gobyalignment" name="goby_alignment" label="${tool.name} on ${on_string} uniq.${result.format}" from_work_dir="gsnap_out.nomapping"> <filter>result['format'] == 'goby'</filter> </data> --> </outputs> <tests> </tests> <help> **What it does** GSNAP_ (Genomic Short-read Nucleotide Alignment Program) is a short read aligner which can align both single- and paired-end reads as short as 14nt and of arbitrarily long length. It can detect short- and long-distance splicing, including interchromosomal splicing, in individual reads, using probabilistic models or a database of known splice sites. Our program also permits SNP-tolerant alignment to a reference space of all possible combinations of major and minor alleles, and can align reads from bisulfite-treated DNA for the study of methylation state. It is developed by Thomas D. Wu of Genentech, Inc. Publication_ citation: Thomas D. Wu, Serban Nacu "Fast and SNP-tolerant detection of complex variants and splicing in short reads. Bioinformatics. 2010 Apr 1;26(7):873-81. Epub 2010 Feb 10. .. _GSNAP: http://research-pub.gene.com/gmap/ .. _Publication: http://bioinformatics.oupjournals.org/cgi/content/full/26/7/873 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2844994/?tool=pubmed ------ **Know what you are doing** .. class:: warningmark You will want to read the README_ .. _README: http://research-pub.gene.com/gmap/src/README ------ **Input formats** Input to GSNAP should be either in FASTQ or FASTA format. The FASTQ input may include quality scores, which will then be included in SAM output, if that output format is selected. For FASTA format, you should include one line per read (or end of a paired-end read). The same FASTA file can have a mixture of single-end and paired-end reads of varying lengths, if desired. Single-end reads: Each FASTA entry should contain one short read per line, like this >Header information AAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA Each short read can have a different length. However, the entire read needs to be on a single line, and may not wrap around multiple lines. If it extends to a second line, GSNAP will think that the read is paired-end. Paired-end reads: Each FASTA entry should contain two short reads, one per line, like this >Header information AAAACATTCTCCTCCGCATAAGCCTAGTAGATTA GGCGTAGGTAGAAGTAGAGGTTAAGGCGCGTCAG By default, the program assumes that the second end is in the reverse complement direction compared with the first end. If they are in the same direction, you may need to use the --circular-input (or -c) flag. ( The Galaxy tool: "FASTA Width formatter" can be used to reformat fasta files to have single line sequences. ) ------ **Output formats in GSNAP** SAM output format Default GSNAP format See the README_ </help> </tool>