Mercurial > repos > jjohnson > defuse
diff README @ 0:b75ea9927793
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author | jjohnson |
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date | Fri, 04 Jan 2013 11:53:33 -0500 |
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children | ffc5e442c1ca |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/README Fri Jan 04 11:53:33 2013 -0500 @@ -0,0 +1,35 @@ +The DeFuse galaxy tool is based on DeFuse_Version_0.5.0 +http://sourceforge.net/apps/mediawiki/defuse/index.php?title=Main_Page + +DeFuse is a software package for gene fusion discovery using RNA-Seq data. The software uses clusters of discordant paired end alignments to inform a split read alignment analysis for finding fusion boundaries. The software also employs a number of heuristic filters in an attempt to reduce the number of false positives and produces a fully annotated output for each predicted fusion. + + +Manual: +http://sourceforge.net/apps/mediawiki/defuse/index.php?title=DeFuse_Version_0.4.2 + +The included tool_dependencies.xml will download and install the defuse code. +It will set the environment variable: "DEFUSE_PATH" to the location of the defuse install. + + +The defuse.pl command relies on a configuration file to specifiy options, the location of reference data, and other applications that it depends upon: bowtie, bowtie-build, samtools, blat, fatotwobit, R, and Rscript. + +The DeFuse galaxy tool can either construct the config.txt file that is mentioned in the defuse manual, or select an existing config.txt file in the users history. +When constructing the config.txt file, the DeFuse tool uses the values selected in: tool-data/defuse.loc +The dictionary field in the tool-data/defuse.loc can be used to set fields in the config.txt file, including the site specific location of reference data and the paths to the other application binaries. +The "Defuse parameter settings" are used to alter options in the config.txt file. + +The DeFuse galaxy tool also generates a bash script to run defuse. +That script will attempt to edit the config.txt file to specifiy any unset paths to applications that defuse relies upon: +bowtie, bowtie-build, samtools, blat, fatotwobit, R, and Rscript +The script uses the using the shell "which" command to discover the application path, so the required applications should in PATH environment variable. + + +Generate Reference Datasets as described in the Manual: + +The manual has detailed instructions on how to set up reference datasets for Human hg19 and hg18. +We were able to follow the same basic procedures to set up a reference for Mouse mm9. + +These datasets should be referenced in the tool-data/defuse.loc file. + + +