Mercurial > repos > jjohnson > bcftools_call
annotate macros.xml @ 0:20b3aaa16d64 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
author | jjohnson |
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date | Sat, 25 Jun 2016 20:46:24 -0400 |
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20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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1 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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2 <macros> |
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3 <token name="@VERSION@">1.3</token> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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4 <xml name="stdio"> |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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5 <stdio> |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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6 <exit_code range="1:" /> |
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7 <exit_code range=":-1" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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8 <regex match="Error:" /> |
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9 <regex match="Exception:" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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10 </stdio> |
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11 </xml> |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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12 <xml name="requirements"> |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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13 <requirements> |
20b3aaa16d64
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14 <requirement type="package" version="1.3">bcftools</requirement> |
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15 <!-- conda dependency --> |
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16 <requirement type="package" version="1.3">htslib</requirement> |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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17 <requirement type="package" version="0.2.6">tabix</requirement> |
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18 <requirement type="package" version="1.2">samtools</requirement> |
20b3aaa16d64
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19 </requirements> |
20b3aaa16d64
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20 </xml> |
20b3aaa16d64
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21 <xml name="version_command"> |
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22 <version_command>bcftools 2>&1 | grep 'Version:'</version_command> |
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23 </xml> |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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24 |
20b3aaa16d64
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25 <xml name="citations"> |
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26 <citations> |
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27 <citation type="doi">10.1093/bioinformatics/btp352</citation> |
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28 <yield /> |
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29 </citations> |
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30 </xml> |
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31 <token name="@BCFTOOLS_WIKI@">https://github.com/samtools/bcftools/wiki</token> |
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32 <token name="@BCFTOOLS_MANPAGE@">http://samtools.github.io/bcftools/bcftools.html</token> |
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33 <token name="@THREADS@"> |
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34 --threads \${GALAXY_SLOTS:-4} |
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35 </token> |
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36 <token name="@PREPARE_ENV@"> |
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37 <![CDATA[ |
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38 export BCFTOOLS_PLUGINS=`which bcftools | sed 's,bin/bcftools,libexec/bcftools,'`; |
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39 ]]> |
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40 </token> |
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41 <xml name="macro_input"> |
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42 <param name="input_file" type="data" format="vcf,vcf_bgzip,bcf,bcf_bgzip" label="VCF/BCF Data" /> |
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43 </xml> |
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44 <token name="@PREPARE_INPUT_FILE@"> |
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45 <![CDATA[ |
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46 ## May need to symlink input if there is an associated |
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47 #set $input_vcf = 'input.vcf.gz' |
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48 #if $input_file.datatype.file_ext == 'vcf' |
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49 bgzip -c "$input_file" > $input_vcf && |
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50 bcftools index $input_vcf && |
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51 #elif $input_file.datatype.file_ext == 'vcf_bgzip' |
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52 ln -s "$input_file" $input_vcf |
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53 #elif $input_file.datatype.file_ext == 'bcf' |
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54 #set $input_vcf = 'input.bcf' |
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55 ln -s "$input_file" $input_vcf && |
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56 #if $input_file.metadata.bcf_index: |
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57 ln -s $input_file.metadata.bcf_index ${input_vcf}.csi && |
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58 #else |
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59 bcftools index $input_vcf && |
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60 #end if |
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61 #elif $input_file.datatype.file_ext == 'bcf_bgzip' |
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62 ln -s "$input_file" $input_vcf |
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63 #end if |
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64 ]]> |
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65 </token> |
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66 <token name="@INPUT_FILE@"> |
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67 $input_vcf |
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68 </token> |
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69 |
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70 <xml name="macro_inputs"> |
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71 <param name="input_files" type="data" format="vcf,bcf" label="Other VCF/BCF Datasets" multiple="True" /> |
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72 </xml> |
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73 <token name="@PREPARE_INPUT_FILES@"> |
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74 <![CDATA[ |
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75 ## May need to symlink input if there is an associated |
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76 #set $input_vcfs = [] |
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77 #set $vcfs_list_file = 'vcfs_list' |
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78 #for (i,input_file) in enumerate($input_files): |
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79 #set $input_vcf = 'input' + str($i) + '.vcf.gz' |
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80 echo '$input_vcf' >> $vcfs_list_file && |
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81 #if $input_file.datatype.file_ext == 'vcf' |
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82 bgzip -c "$input_file" > $input_vcf && |
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83 bcftools index $input_vcf && |
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84 #elif $input_file.datatype.file_ext == 'vcf_bgz' |
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85 ln -s "$input_file" $input_vcf |
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86 #elif $input_file.datatype.file_ext == 'bcf' |
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87 #set $input_vcf = 'input' + str($i) + '.bcf.gz' |
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88 ## bgzip -c "$input_file" > $input_vcf && |
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89 ln -s "$input_file" $input_vcf && |
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90 #if $input_file.metadata.bcf_index: |
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91 ln -s $input_file.metadata.bcf_index ${input_vcf}.csi && |
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92 #else |
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93 bcftools index $input_vcf && |
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94 #end if |
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95 #elif $input_file.datatype.file_ext == 'bcfvcf_bgz' |
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96 ln -s "$input_file" $input_vcf && |
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97 #end if |
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98 $input_vcfs.append($input_vcf) |
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99 #end for |
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100 ]]> |
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101 </token> |
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102 <token name="@INPUT_FILES@"> |
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103 #echo ' '.join($input_vcfs)# |
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104 </token> |
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105 <token name="@INPUT_LIST_FILE@"> |
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106 $vcfs_list_file |
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107 </token> |
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108 |
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109 <xml name="macro_fasta_ref"> |
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110 <param name="fasta_ref" type="data" format="data" label="Fasta Ref" optional="True" help="reference sequence in fasta format" /> |
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111 </xml> |
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112 <token name="@PREPARE_FASTA_REF@"> |
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113 <![CDATA[ |
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114 #set $input_fa_ref = None |
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115 #if 'fasta_ref' in $section and $section.fasta_ref: |
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116 #set $input_fa_ref = 'ref.fa' |
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117 ln -s $section.fasta_ref $input_fa_ref && |
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118 samtools faidx $input_fa_ref && |
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119 #end if |
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120 ]]> |
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121 </token> |
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122 <token name="@FASTA_REF@"> |
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123 #if $input_fa_ref is not None: |
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124 --fasta-ref "$input_fa_ref" |
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125 #elif 'fasta_ref' in $section and $section.fasta_ref: |
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126 --fasta-ref "${section.fasta_ref}" |
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127 #end if |
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128 </token> |
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129 |
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130 <xml name="macro_ref_fasta"> |
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131 <conditional name="reference_source"> |
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132 <param name="reference_source_selector" type="select" label="Choose the source for the reference genome"> |
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133 <option value="cached">Locally cached</option> |
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134 <option value="history">History</option> |
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135 </param> |
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136 <when value="cached"> |
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137 <param name="ref_file" type="select" label="Select reference genome"> |
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138 <options from_data_table="fasta_indexes"> |
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139 <!--<filter type="data_meta" key="dbkey" ref="input_bam" column="value"/>--> |
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140 </options> |
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141 <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/> |
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142 </param> |
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143 </when> |
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144 <when value="history"> <!-- FIX ME!!!! --> |
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145 <param name="ref_file" type="data" format="fasta" label="Using reference file" /> |
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146 </when> |
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147 </conditional> |
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148 </xml> |
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149 |
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150 |
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151 <xml name="macro_AF_file"> |
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152 <param name="AF_file" type="data" format="data" label="Af File" optional="True" help="read allele frequencies from file (CHR\tPOS\tREF,ALT\tAF)" /> |
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153 </xml> |
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154 <!-- This may need to bgzip and tabix the file --> |
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155 <token name="@PREPARE_AF_FILE@"> |
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156 <![CDATA[ |
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157 #if 'AF_file' in $section and $section.AF_file: |
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158 #pass |
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159 #end if |
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160 ]]> |
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161 </token> |
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162 <token name="@AF_FILE@"> |
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163 #if 'AF_file' in $section and $section.AF_file: |
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164 --AF-file "${section.AF_file}" |
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165 #end if |
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166 </token> |
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167 |
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168 <xml name="macro_estimate_AF"> |
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169 <param name="estimate_AF" type="data" format="data" label="Estimate Af" optional="True" help="calculate AC,AN counts on the fly, using either all samples ("-") or samples listed in <file>" /> |
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170 </xml> |
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171 <token name="@ESTIMATE_AF@"> |
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172 #if 'estimate_AF' in $section and $section.estimate_AF: |
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173 --estimate-AF "${section.estimate_AF}" |
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174 #end if |
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175 </token> |
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176 |
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177 <xml name="macro_exons_file"> |
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178 <param name="exons_file" type="data" format="tabular" label="exons file" optional="True" help="tab-delimited file with exons for indel frameshifts (chr,from,to; 1-based, inclusive, bgzip compressed)" /> |
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179 </xml> |
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180 <token name="@PREPARE_EXONS_FILE@"> |
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181 <![CDATA[ |
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182 #set $exons_path = None |
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183 #if 'exons_file' in $section and $section.exons_file: |
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184 #set $exons_path = 'exons_file.tab.gz' |
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185 bgzip -c "$section.exons_file" > $exons_path && |
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186 tabix -s 1 -b 2 -e 3 $exons_path && |
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187 #end if |
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188 ]]> |
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189 </token> |
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190 <token name="@EXONS_FILE@"> |
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191 #if 'exons_file' in $section and $section.exons_file: |
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192 --exons $exons_path |
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193 #end if |
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194 </token> |
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195 |
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196 <xml name="macro_ploidy_file"> |
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197 <param name="ploidy_file" type="data" format="tabular" label="Ploidy file" optional="True" help="tab-delimited list of CHROM,FROM,TO,SEX,PLOIDY" /> |
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198 </xml> |
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199 <token name="@PLOIDY_FILE@"> |
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200 #if 'ploidy_file' in $section and $section.ploidy_file: |
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201 --ploidy "${section.ploidy_file}" |
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202 #end if |
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203 </token> |
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204 |
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205 <xml name="macro_collapse_opt_none"> |
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206 <option value="none">none - require the exact same set of alleles in all files</option> |
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207 </xml> |
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208 <xml name="macro_collapse_opt_id"> |
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209 <option value="id">id - only records with identical ID column are compatible. </option> |
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210 </xml> |
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211 <xml name="macro_collapse"> |
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212 <param name="collapse" type="select" label="Collapse" optional="True" help="Controls how to treat records with duplicate positions and defines compatible records across multiple input files"> |
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213 <option value="snps">snps - allow different alleles, as long as they all are SNPs</option> |
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214 <option value="indels">indels - allow different alleles, as long as they all are indels</option> |
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215 <option value="both">both - indels and snps </option> |
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216 <option value="some">some - at least some of the ALTs must match</option> |
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217 <option value="any">any - any combination of alleles</option> |
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218 <yield/> |
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219 </param> |
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220 </xml> |
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221 <token name="@COLLAPSE@"> |
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222 #if $section.collapse: |
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223 --collapse "${section.collapse}" |
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224 #end if |
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225 </token> |
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226 |
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227 <xml name="macro_apply_filters"> |
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228 <param name="apply_filters" type="text" value="" label="Apply Filters" optional="true" |
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229 help="(-f --apply-filters) Skip sites where FILTER column does not contain any of the strings listed (e.g. "PASS,.")"> |
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230 <validator type="regex" message="FILTER terms separated by commas">^([^ \t\n\r\f\v,]+(,[^ \t\n\r\f\v,]+)*)?$</validator> |
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231 </param> |
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232 </xml> |
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233 <token name="@APPLY_FILTERS@"> |
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234 #if $section.apply_filters: |
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235 --apply-filters "${section.apply_filters}" |
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236 #end if |
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237 </token> |
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238 |
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239 <xml name="macro_select_output_type"> |
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240 <param name="output_type" type="select"> |
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241 <option value="b">compressed BCF</option> |
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242 <!-- no galaxy datatypes for these |
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243 <option value="u">uncompressed BCF</option> |
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244 <option value="z">compressed VCF</option> |
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245 --> |
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246 <option value="v">uncompressed VCF</option> |
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247 </param> |
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248 </xml> |
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249 <token name="@OUTPUT_TYPE@"> |
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250 #if str($output_type) != "__none__": |
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251 --output-type "${output_type}" |
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252 #end if |
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253 </token> |
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254 |
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255 <xml name="macro_vcf_output"> |
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256 <data name="output_file" format="vcf"> |
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257 <change_format> |
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258 <when input="output_type" value="b" format="bcf" /> |
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259 <when input="output_type" value="u" format="bcf" /> |
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260 <when input="output_type" value="z" format="vcf_bgzip" /> |
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261 <when input="output_type" value="v" format="vcf" /> |
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262 </change_format> |
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263 </data> |
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264 </xml> |
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265 |
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266 <xml name="macro_regions"> |
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267 <conditional name="regions"> |
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268 <param name="regions_src" type="select" label="Regions"> |
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269 <option value="__none__">None</option> |
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270 <option value="regions">regions</option> |
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271 <option value="regions_file">regions-file</option> |
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272 </param> |
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273 <when value="__none__"/> |
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274 <when value="regions"> |
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275 <param name="regions" type="text" value="" label="restrict to comma-separated list of regions" optional="true" |
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276 help="Each region is specifed as: chr or chr:pos or chr:from-to"> |
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277 <validator type="regex" message="">^(\w+(:\d+(-\d+)?)?(,\w+(:\d+(-\d+)?)?)*)?$</validator> |
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278 </param> |
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279 </when> |
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280 <when value="regions_file"> |
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281 <param name="regions_file" type="data" format="vcf,bed,tabular" label="Regions File" optional="True" help="restrict to regions listed in a file" /> |
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282 </when> |
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283 </conditional> |
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284 </xml> |
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285 <token name="@REGIONS@"> |
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286 #if $section.regions.regions_src == 'regions' and $section.regions.regions != '': |
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287 --regions "$section.regions.regions" |
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288 #elif $section.regions.regions_src == 'regions_file' and $section.regions.regions_file: |
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289 --regions-file "$section.regions.regions_file" |
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290 #end if |
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291 </token> |
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292 |
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293 <xml name="macro_targets_file"> |
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294 <param name="targets_file" type="data" format="tabular" label="Targets File" help="restrict to targets listed in a file" > |
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295 <yield/> |
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296 </param> |
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297 <param name="invert_targets_file" type="boolean" truevalue="^" falsevalue="" label="Invert Targets" help="inverts the query/filtering applied by the target file selection" /> |
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298 </xml> |
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299 <token name="@PREPARE_TARGETS_FILE@"> |
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300 <![CDATA[ |
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301 #set $targets_path = None |
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302 #if 'targets' in $section |
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303 #if $section.targets.targets_src == 'targets_file': |
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304 #set $targets_path = 'targets_file.tab.gz' |
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305 bgzip -c "$section.targets.targets_file" > $targets_path && |
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306 tabix -s 1 -b 2 -e 2 $targets_path && |
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307 #end if |
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308 #elif $tgts_sec.targets_file: |
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309 #set $targets_path = 'targets_file.tab.gz' |
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310 bgzip -c "$section.targets_file" > $targets_path && |
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311 tabix -s 1 -b 2 -e 2 $targets_path && |
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312 #end if |
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313 ]]> |
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314 </token> |
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315 <token name="@TARGETS_FILE@"> |
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316 <![CDATA[ |
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317 #if $targets_path is not None: |
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318 --targets-file "${section.invert_targets_file}${targets_path}" |
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319 #elif $section.targets_file: |
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320 --targets-file "${section.invert_targets_file}${section.targets_file}" |
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321 #end if |
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322 ]]> |
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323 </token> |
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324 |
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325 <xml name="macro_targets"> |
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326 <conditional name="targets"> |
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327 <param name="targets_src" type="select" label="Targets"> |
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328 <option value="__none__">None</option> |
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329 <option value="targets">targets</option> |
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330 <option value="targets_file">targets-file</option> |
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331 </param> |
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332 <when value="__none__"/> |
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333 <when value="targets"> |
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334 <param name="targets" type="text" value="" label="Restrict to comma-separated list of targets" optional="true" |
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335 help="Each target is specifed as: chr or chr:pos or chr:from-to"> |
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336 <validator type="regex" message="">^(\w+(:\d+(-\d+)?)?(,\w+(:\d+(-\d+)?)?)*)?$</validator> |
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337 </param> |
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338 <param name="invert_targets_file" type="boolean" truevalue="^" falsevalue="" label="Invert Targets" help="inverts the query/filtering applied by the targets" /> |
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339 </when> |
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340 <when value="targets_file"> |
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341 <expand macro="macro_targets_file"> |
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342 <optional>true</optional> |
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343 </expand> |
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344 </when> |
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345 </conditional> |
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346 </xml> |
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347 <token name="@TARGETS@"> |
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348 <![CDATA[ |
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349 #if $targets_path: |
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350 --targets-file "${section.targets.invert_targets_file}${targets_path}" |
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351 #else: |
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352 #if $section.targets.targets_src == 'targets' and $section.targets.targets != '': |
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353 --targets "${section.targets.invert_targets_file}${section.targets.targets}" |
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354 #elif $section.targets.targets_src == 'targets_file' and $section.targets.targets_file: |
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355 --targets-file "${section.targets.invert_targets_file}${section.targets.targets_file}" |
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356 #end if |
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357 #end if |
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358 ]]> |
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359 </token> |
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360 |
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361 <xml name="macro_samples"> |
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362 <param name="samples" type="text" value="" label="Samples" optional="true" |
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363 help="(-s) comma separated list of samples to annotate (or exclude with "^" prefix)"> |
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364 <validator type="regex" message="">^(\w+(,\w+)*)?$</validator> |
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365 </param> |
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366 <param name="invert_samples" type="boolean" truevalue="^" falsevalue="" checked="false" label="Invert Samples" |
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367 help="inverts the query/filtering applied by Samples" /> |
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368 <param name="samples_file" type="data" format="tabular" label="Samples File" optional="True" |
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369 help="(-S) file of samples to include" /> |
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370 <param name="invert_samples_file" type="boolean" truevalue="^" falsevalue="" checked="false" label="Invert Samples File" |
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371 help="inverts the query/filtering applied by Samples File" /> |
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372 </xml> |
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373 <token name="@SAMPLES@"> |
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374 #set $samples_defined = False |
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375 #if str($section.samples) != '': |
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376 #set $samples_defined = True |
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377 --samples "${section.invert_samples}${section.samples}" |
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378 #end if |
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379 #if $section.samples_file: |
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380 #set $samples_defined = True |
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381 --samples-file "${section.invert_samples_file}${section.samples_file}" |
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382 #end if |
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383 </token> |
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384 |
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385 <xml name="macro_sample"> |
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386 <param name="sample" type="text" label="Sample" optional="True" help="apply variants of the given sample" /> |
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387 </xml> |
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388 <token name="@SAMPLE@"> |
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389 #if $section.sample: |
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390 --sample "${section.sample}" |
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391 #end if |
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392 </token> |
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393 |
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394 |
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395 <xml name="macro_include"> |
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396 <param name="include" type="text" label="Include" optional="True" help="(-i) select sites for which the expression is true"> |
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397 <validator type="regex" message="Single quote not allowed">^[^']*$</validator> |
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398 <sanitizer sanitize="False"/> |
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399 </param> |
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400 </xml> |
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401 <token name="@INCLUDE@"> |
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402 #if $section.include: |
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403 --include '${section.include}' |
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404 #end if |
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405 </token> |
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406 |
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407 <xml name="macro_exclude"> |
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408 <param name="exclude" type="text" label="Exclude" optional="True" help="(-e) exclude sites for which the expression is true"> |
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409 <validator type="regex" message="Single quote not allowed">^[^']*$</validator> |
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410 <sanitizer sanitize="False"/> |
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411 </param> |
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412 </xml> |
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413 <token name="@EXCLUDE@"> |
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414 #if $section.exclude: |
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415 --exclude '${section.exclude}' |
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416 #end if |
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417 </token> |
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418 |
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419 <xml name="macro_columns"> |
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420 <param name="columns" type="text" value="" label="Columns" optional="true" |
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421 help="list of columns in the annotation file, e.g. CHROM,POS,REF,ALT,-,INFO/TAG. See man page for details"> |
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422 <validator type="regex" message="COLUMN names separated by commas">^([^,]+(,[^,]+)*)?$</validator> |
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423 </param> |
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424 </xml> |
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425 <token name="@COLUMNS@"> |
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426 #if $section.columns != '': |
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427 --columns "${section.columns}" |
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428 #end if |
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429 </token> |
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430 |
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431 <xml name="macro_haploid2diploid"> |
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432 <param name="haploid2diploid" type="boolean" truevalue="--haploid2diploid" falsevalue="" label="Haploid2Diploid" help="convert haploid genotypes to diploid homozygotes" /> |
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433 </xml> |
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434 |
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435 <xml name="macro_vcf_ids"> |
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436 <param name="vcf_ids" type="boolean" truevalue="--vcf-ids" falsevalue="" label="Vcf Ids" help="output VCF IDs instead of CHROM:POS_REF_ALT" /> |
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437 </xml> |
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438 <token name="@VCF_IDS@"> |
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439 ${section.vcf_ids} |
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440 </token> |
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441 |
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442 <token name="@OUTPUT_HELP@"> |
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443 <![CDATA[ |
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444 Output Type |
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445 ----------- |
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446 |
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447 Output compressed BCF (b), or uncompressed VCF (v). |
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448 Use the BCF option when piping between bcftools subcommands to speed up |
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449 performance by removing unecessary compression/decompression |
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450 and VCF<->BCF conversion. |
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451 |
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452 This Galaxy tool recommends using the compressed BCF format |
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453 as piping is not implemented, and uncompressed data would |
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454 use unnecessary amounts of space. |
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455 |
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456 ]]></token> |
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457 <token name="@REGIONS_HELP@"> |
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458 <![CDATA[ |
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459 Region Selections |
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460 ----------------- |
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461 |
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462 Regions can be specified in a VCF, |
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463 BED, or tab-delimited file (the default). The columns of the |
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464 tab-delimited file are: CHROM, POS, and, optionally, POS_TO, |
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465 where positions are 1-based and inclusive. Uncompressed |
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466 files are stored in memory, while bgzip-compressed and |
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467 tabix-indexed region files are streamed. Note that sequence |
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468 names must match exactly, "chr20" is not the same as "20". |
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469 Also note that chromosome ordering in FILE will be |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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470 respected, the VCF will be processed in the order in which |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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471 chromosomes first appear in FILE. However, within |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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472 chromosomes, the VCF will always be processed in ascending |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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473 genomic coordinate order no matter what order they appear in |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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474 FILE. Note that overlapping regions in FILE can result in |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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475 duplicated out of order positions in the output. This option |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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476 requires indexed VCF/BCF files. |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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477 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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478 ]]></token> |
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479 <token name="@TARGETS_HELP@"><![CDATA[ |
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480 Targets |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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481 ------- |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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482 |
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483 Similar to regions, but the next position is accessed by streaming the whole |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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484 VCF/BCF rather than using the tbi/csi index. Both regions and targets options can be |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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485 applied simultaneously: regions uses the index to jump to a region and targets discards |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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486 positions which are not in the targets. Unlike regions, targets can be prefixed with |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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487 "^" to request logical complement. For example, "^X,Y,MT" indicates that |
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488 sequences X, Y and MT should be skipped. Yet another difference between the two |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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489 is that regions checks both start and end positions of indels, whereas targets checks |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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490 start positions only. |
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491 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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492 For the bcftools call command, with the option -C alleles, third column of the |
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493 targets file must be comma-separated list of alleles, starting with the |
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494 reference allele. Note that the file must be compressed and index. Such a file |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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495 can be easily created from a VCF using:: |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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496 |
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497 bcftools query -f'%CHROM\t%POS\t%REF,%ALT\n' file.vcf | bgzip -c > als.tsv.gz && tabix -s1 -b2 -e2 als.tsv.gz |
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498 ]]> |
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499 <!-- TODO: galaxy-ify --> |
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500 </token> |
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501 |
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502 |
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503 <token name="@COLLAPSE_HELP@"> |
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504 Collapse |
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505 -------- |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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506 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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507 Controls how to treat records with duplicate positions and defines compatible |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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508 records across multiple input files. Here by "compatible" we mean records which |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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509 should be considered as identical by the tools. For example, when performing |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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510 line intersections, the desire may be to consider as identical all sites with |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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511 matching positions (bcftools isec -c all), or only sites with matching variant |
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512 type (bcftools isec -c snps -c indels), or only sites with all alleles |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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513 identical (bcftools isec -c none). |
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514 |
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515 |
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516 +------------+----------------------------------------------------------------+ |
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517 | Flag value | Result | |
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518 +============+================================================================+ |
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519 | none | only records with identical REF and ALT alleles are compatible | |
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520 +------------+----------------------------------------------------------------+ |
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521 | some | only records where some subset of ALT alleles match are | |
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522 | | compatible | |
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523 +------------+----------------------------------------------------------------+ |
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524 | all | all records are compatible, regardless of whether the ALT | |
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525 | | alleles match or not. In the case of records with the same | |
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526 | | position, only the first wil lbe considered and appear on | |
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527 | | output. | |
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528 +------------+----------------------------------------------------------------+ |
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529 | snps | any SNP records are compatible, regardless of whether the ALT | |
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530 | | alleles match or not. For duplicate positions, only the first | |
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531 | | SNP record will be considered and appear on output. | |
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532 +------------+----------------------------------------------------------------+ |
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533 | indels | all indel records are compatible, regardless of whether the | |
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534 | | REF and ALT alleles match or not. For duplicate positions, | |
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535 | | only the first indel record will be considered and appear on | |
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536 | | output. | |
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537 +------------+----------------------------------------------------------------+ |
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538 | both | abbreviation of "-c indels -c snps" | |
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539 +------------+----------------------------------------------------------------+ |
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540 | id | only records with identical ID column are compatible. | |
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541 | | Supportedby bcftools merge only. | |
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542 +------------+----------------------------------------------------------------+ |
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543 |
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544 </token> |
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545 |
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546 <token name="@EXPRESSIONS_HELP@"> |
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547 <![CDATA[ |
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548 Expressions |
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parents:
diff
changeset
|
549 ----------- |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
550 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
551 Valid expressions may contain: |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
552 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
553 - numerical constants, string constants |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
554 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
555 :: |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
556 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
557 1, 1.0, 1e-4 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
558 "String" |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
559 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
560 - arithmetic operators |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
561 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
562 :: |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
563 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
564 +,*,-,/ |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
565 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
566 - comparison operators |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
567 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
568 :: |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
569 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
570 == (same as =), >, >=, <=, <, != |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
571 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
572 - regex operators "~" and its negation "!~" |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
573 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
574 :: |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
575 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
576 INFO/HAYSTACK ~ "needle" |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
577 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
578 - parentheses |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
579 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
580 :: |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
581 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
582 (, ) |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
583 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
584 - logical operators |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
585 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
586 :: |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
587 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
588 && (same as &), ||, | |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
589 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
590 - INFO tags, FORMAT tags, column names |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
591 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
592 :: |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
593 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
594 INFO/DP or DP |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
595 FORMAT/DV, FMT/DV, or DV |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
596 FILTER, QUAL, ID, REF, ALT[0] |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
597 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
598 - 1 (or 0) to test the presence (or absence) of a flag |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
599 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
600 :: |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
601 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
602 FlagA=1 && FlagB=0 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
603 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
604 - "." to test missing values |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
605 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
606 :: |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
607 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
608 DP=".", DP!=".", ALT="." |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
609 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
610 - missing genotypes can be matched regardless of phase and ploidy (".|.", "./.", ".") using this expression |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
611 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
612 :: |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
613 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
614 GT="." |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
615 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
616 - TYPE for variant type in REF,ALT columns (indel,snp,mnp,ref,other) |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
617 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
618 :: |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
619 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
620 TYPE="indel" | TYPE="snp" |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
621 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
622 - array subscripts, "*" for any field |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
623 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
624 :: |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
625 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
626 (DP4[0]+DP4[1])/(DP4[2]+DP4[3]) > 0.3 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
627 DP4[*] == 0 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
628 CSQ[*] ~ "missense_variant.*deleterious" |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
629 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
630 - function on FORMAT tags (over samples) and INFO tags (over vector fields) |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
631 |
20b3aaa16d64
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
jjohnson
parents:
diff
changeset
|
632 :: |
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633 |
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634 MAX, MIN, AVG, SUM, STRLEN, ABS |
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635 |
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636 - variables calculated on the fly if not present: number of alternate alleles; number of samples; count of alternate alleles; minor allele count (similar to AC but is always smaller than 0.5); frequency of alternate alleles (AF=AC/AN); frequency of minor alleles (MAF=MAC/AN); number of alleles in called genotypes |
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637 |
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638 :: |
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639 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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640 N_ALT, N_SAMPLES, AC, MAC, AF, MAF, AN |
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641 |
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642 **Notes:** |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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643 |
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644 - String comparisons and regular expressions are case-insensitive |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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645 - If the subscript "*" is used in regular expression search, the whole field |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bcftools commit 21c66fb27c7e2fd21c7f7607b3b29e77e64fb86d-dirty
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646 is treated as one string. For example, the regex ``STR[*]~"B,C"`` will be |
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647 true for the string vector INFO/STR=AB,CD. |
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648 - Variables and function names are case-insensitive, but not tag names. For |
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649 example, "qual" can be used instead of "QUAL", "strlen()" instead of |
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650 "STRLEN()" , but not "dp" instead of "DP". |
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651 |
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652 **Examples:** |
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653 |
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654 :: |
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655 |
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656 MIN(DV)>5 |
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657 MIN(DV/DP)>0.3 |
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658 MIN(DP)>10 & MIN(DV)>3 |
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659 FMT/DP>10 & FMT/GQ>10 .. both conditions must be satisfied within one sample |
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660 FMT/DP>10 && FMT/GQ>10 .. the conditions can be satisfied in different samples |
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661 QUAL>10 | FMT/GQ>10 .. selects only GQ>10 samples |
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662 QUAL>10 || FMT/GQ>10 .. selects all samples at QUAL>10 sites |
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663 TYPE="snp" && QUAL>=10 && (DP4[2]+DP4[3] > 2) |
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664 MIN(DP)>35 && AVG(GQ)>50 |
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665 ID=@file .. selects lines with ID present in the file |
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666 ID!=@~/file .. skip lines with ID present in the ~/file |
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667 MAF[0]<0.05 .. select rare variants at 5% cutoff |
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668 |
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669 ]]></token> |
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670 |
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671 |
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672 |
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673 |
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674 </macros> |