annotate unified-histogram.py @ 0:8464186c8503 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
author iuc
date Wed, 09 Aug 2017 09:52:31 -0400
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8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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1 #!/usr/bin/env python
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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2 import logging
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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3 import sys
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5 import wiggle
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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6 from BCBio import GFF
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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8 logging.basicConfig(level=logging.INFO)
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9 log = logging.getLogger()
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12 MODE = sys.argv[1]
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14 # Pair up (file, extension) pairs from sys.argv
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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15 files = zip(sys.argv[2:][0::2], sys.argv[2:][1::2])
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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17 # Our output data structure. This could be much more efficient.
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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18 data = {}
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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21 def bed(idx, path):
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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22 # chrom - The name of the chromosome (e.g. chr3, chrY, chr2_random) or scaffold (e.g. scaffold10671).
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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23 # chromStart - The starting position of the feature in the chromosome or scaffold. The first base in a chromosome is numbered 0.
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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24 # chromEnd - The ending position of the feature in the chromosome or scaffold. The chromEnd base is not included in the display of the feature. For example, the first 100 bases of a chromosome are defined as chromStart=0, chromEnd=100, and span the bases numbered 0-99.
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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25 # name - Defines the name of the BED line. This label is displayed to the left of the BED line in the Genome Browser window when the track is open to full display mode or directly to the left of the item in pack mode.
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26 # score - A score between 0 and 1000. If the track line useScore attribute is set to 1 for this annotation data set, the score value will determine the level of gray in which this feature is displayed (higher numbers = darker gray). This table shows the Genome Browser's translation of BED score values into shades of gray:
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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27 # strand - Defines the strand - either '+' or '-'.
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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28 # thickStart - The starting position at which the feature is drawn thickly (for example, the start codon in gene displays). When there is no thick part, thickStart and thickEnd are usually set to the chromStart position.
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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29 # thickEnd - The ending position at which the feature is drawn thickly (for example, the stop codon in gene displays).
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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30 # itemRgb - An RGB value of the form R,G,B (e.g. 255,0,0). If the track line itemRgb attribute is set to "On", this RBG value will determine the display color of the data contained in this BED line. NOTE: It is recommended that a simple color scheme (eight colors or less) be used with this attribute to avoid overwhelming the color resources of the Genome Browser and your Internet browser.
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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32 with open(path, 'r') as handle:
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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33 for line in handle:
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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34 lineData = line.strip().split()
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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35 chrom = lineData[0]
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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36 chromStart = lineData[1]
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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37 chromEnd = lineData[2]
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38
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39 if chrom not in data:
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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40 data[chrom] = {}
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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41
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42 for i in range(chromStart, chromEnd):
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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43 if i not in data[chrom]:
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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44 data[chrom][i] = {}
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45
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46 data[chrom][i][idx] = lineData[5]
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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47
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48
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49 # Handlers
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50 def gff3(idx, path):
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51 for record in GFF.parse(path):
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52 if len(record.features) == 0:
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53 continue
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54
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55 if record.id not in data:
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56 data[record.id] = {}
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57
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58 for feature in record.features:
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59 if 'score' in feature.qualifiers:
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60 for i in range(feature.location.start, feature.location.end):
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61 if i not in data[record.id]:
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62 data[record.id][i] = {}
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63
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64 data[record.id][i][idx] = feature.qualifiers['score'][0]
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65
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67 def wig(idx, path):
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68 walker = wiggle.Wiggle()
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69 with open(path, 'r') as handle:
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70 for region, position, value in walker.walk(handle):
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71 if region not in data:
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72 data[region] = {}
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73
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74 if position not in data[region]:
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75 data[region][position] = {}
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76
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77 data[region][position][idx] = value
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78
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79
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80 if __name__ == '__main__':
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81 mode_tiles_possible = True
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82
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83 for idx, (file_path, file_type) in enumerate(files):
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84 log.info("Processing %s.%s", file_path, file_type)
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85
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86 if file_type in globals():
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87 func = globals()[file_type]
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88 func(idx, file_path)
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89
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90 if file_type == 'wig':
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91 mode_tiles_possible = False
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92
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93 if MODE == 'tile' and not mode_tiles_possible:
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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94 raise Exception("You requested a 'tile' plot with wig data, which is impossible")
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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95
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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96 # Max number of files
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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97 max_idx = range(len(files))
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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98
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99 serialized_values = None
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
iuc
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100 region_start, region_end = (None, None)
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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101
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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102 for genome in data:
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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103 for position in sorted(data[genome]):
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
iuc
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104 values = [
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
iuc
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105 '' if x not in data[genome][position] else data[genome][position][x]
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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106 for x in max_idx
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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107 ]
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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108 if serialized_values is None:
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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109 serialized_values = values
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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110 if region_start is None:
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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111 region_start = position
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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112 region_end = position
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
iuc
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113
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
iuc
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114 if values == serialized_values:
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
iuc
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115 region_end = position
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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116 else:
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
iuc
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117 if MODE == 'histogram':
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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118 # histogram
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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119 # hs4 0 1999999 5.0000,3.0000,1.0000,19.0000
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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120 sys.stdout.write(' '.join(
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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121 (genome, str(region_start), str(region_end), ','.join(map(str, values)))
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
iuc
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122 ) + '\n')
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
iuc
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123 elif MODE == 'heatmap':
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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124 # heatmap
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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125 # hs1 2000000 3999999 0.0000 id=hs4
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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126 # hs1 4000000 5999999 2.0000 id=hs1
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127 # hs1 4000000 5999999 0.0000 id=hs2
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128 # hs1 4000000 5999999 0.0000 id=hs3
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129 # hs1 4000000 5999999 0.0000 id=hs4
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130 # hs1 6000000 7999999 4.0000 id=hs2
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131 for x in max_idx:
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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132 if x in data[genome][position]:
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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133 sys.stdout.write(' '.join(
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
iuc
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134 (genome, str(region_start), str(region_end), data[genome][position][x], 'id=hm%s' % x)
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
iuc
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135 ) + '\n')
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136 else:
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137 sys.stdout.write(' '.join(
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
iuc
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138 (genome, str(region_start), str(region_end), 0.0, 'id=hm%s' % x)
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
iuc
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139 ) + '\n')
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
iuc
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140 elif MODE == 'line':
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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141 # multiple=False
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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142 sys.stdout.write(' '.join(
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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143 (genome, str(region_start), str(region_end), data[genome][position][0])
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
iuc
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144 ) + '\n')
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
iuc
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145 elif MODE == 'scatter':
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iuc
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146 # multiple=False
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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diff changeset
147 sys.stdout.write(' '.join(
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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148 (genome, str(region_start), str(region_end), data[genome][position][0])
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iuc
parents:
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149 ) + '\n')
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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150
8464186c8503 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/circos commit a41493893bdcbe330434db9c5851719012b62fa8
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diff changeset
151 # Update start of next array
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diff changeset
152 region_start = position
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153 region_end = position
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diff changeset
154 # And update with new array
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155 serialized_values = values