Mercurial > repos > iuc > biotradis

annotate bacteria_tradis.xml @ 4:86c8fb3bb521 draft default tip

Find changesets by keywords (author, files, the commit message), revision number or hash, or revset expression.
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 597c82a8fa756e68828ef201de7bb7e69d2c50bd
author iuc
date Mon, 04 Jul 2022 07:28:50 +0000
parents 476d4cefec3a
children
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
rev   line source
1
e8cb43bd2fe4 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 61c83ac999a040604915ff7cf92670b2635b967e"
iuc
parents: 0
diff changeset
1 <tool id="bacteria_tradis" name="Bio-TraDis reads to counts" version="@TOOL_VERSION@+galaxy@VERSION@">
0
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
2 <description></description>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
3 <macros>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
4 <import>macros.xml</import>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
5 </macros>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
6 <expand macro="requirements" />
2
d1278922a88e "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 6a29aaead9d6b0f69ef70113df58047877fc6ce5"
iuc
parents: 1
diff changeset
7 <command detect_errors="aggressive">
0
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
8 <![CDATA[
3
476d4cefec3a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 89fb9733c11dd6bda00f7cd377de461a045ebe88"
iuc
parents: 2
diff changeset
9 cp '${input_ref}' reference.fa &&
0
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
10 ls '${input_fastq}' > file.txt &&
3
476d4cefec3a "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 89fb9733c11dd6bda00f7cd377de461a045ebe88"
iuc
parents: 2
diff changeset
11 bacteria_tradis -v -f file.txt -r reference.fa
0
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
12 #if str($map_parameters.map_options) == "modify":
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
13
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
14 #if str($map_parameters.set_kmers_options.set) == "yes":
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
15 --smalt_k '$map_parameters.set_kmers_options.kmer_length'
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
16 --smalt_s '$map_parameters.set_kmers_options.step_size'
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
17 #end if
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
18
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
19 --smalt_y '$map_parameters.min_percentage'
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
20 --smalt_r '$map_parameters.duplicate_reads'
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
21 -m '$map_parameters.min_quality'
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
22
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
23 #end if
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
24
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
25 #if str($tranposon_tag.use) == "yes":
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
26 -m '$tranposon_tag.nb_mismatches'
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
27 -t '$tranposon_tag.sequence'
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
28 #end if
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
29 2>&1
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
30 ]]>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
31 </command>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
32
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
33 <inputs>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
34 <param name="input_fastq" type="data" format="fastq" label="Fastq file containing TraDis reads"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
35 <param name="input_ref" type="data" format="fasta" label="Fasta File of the reference Genome"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
36
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
37 <conditional name="map_parameters">
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
38 <param name="map_options" type="select" label="Mapping Parameters" help="By default, the bacteria_tradis pipeline determines appropriate read mapping parameters automatically from the length of the first read in the fastq file. These parameters have been tested for data issue from TraDIS protocol of Barquist et al.">
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
39 <option value="default" selected="true">Use Default Parameters</option>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
40 <option value="modify">Set Mapping parameters</option>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
41
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
42 </param>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
43 <when value="modify">
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
44
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
45 <conditional name="set_kmers_options">
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
46 <param name="set" type="boolean" label="Modify kmers parameters" truevalue='yes' falsevalue='no' />
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
47 <when value="yes">
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
48 <param name="kmer_length" type="integer" value="" min="9" max="20" label=" Length of kmers hashed (--smalt_k)" help=" The minimum length of an exact match between a read and the genome needed to trigger an alignment attempt. Appropriate values are between ~10 and 20 for bacterial genomes depending on read length. Lower values lead to increased sensitivity at the expense of runtime." />
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
49 <param name="step_size" type="integer" value="" min="1" max="15" label="Step size for smalt kmers (--smalt_s)" help=" Distance between the start of hashed kmers. Appropriate values are between 1 and ~15, but should be less than --smalt_k to ensure kmers overlap. Lower values lead to increased sensitivity at the expense of runtime." />
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
50 </when>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
51 <when value="no">
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
52 </when>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
53 </conditional>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
54
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
55 <param name="min_percentage" type="float" value="0.96" min="0" max="1" label="Minimum percentage of identical bases between read and reference (--smalt_y)" help="May be lowered to improve sensitivity in the case of low quality or short reads." />
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
56 <param name="duplicate_reads" type="boolean" truevalue="-1" falsevalue="0" label="Randomly assign position to reads that align in multiple location (--smalt_r)" help="If not, reads mapping in multiples positions are ignored" />
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
57 <param name="min_quality" type="integer" value="30" label="Minimum mapping quality score (-m) " help="Multi-mapping reads have a quality score of 0 by definition, so this parameter needs to be set to 0 for these reads to be properly processed. Can be lowered without dramatically affecting results in most cases, particularly if --smalt_y is set reasonably." />
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
58
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
59 </when>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
60 <when value="default">
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
61 </when>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
62 </conditional>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
63
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
64 <conditional name="tranposon_tag">
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
65 <param name="use" type="boolean" truevalue="yes" falsevalue="no" label="Search for a tranposon tag" help="Use with data containing a transposon tag attached to the reads. Only reads containing the transposon tag will be processed, and the tag will be removed before mapping." />
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
66
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
67 <when value="yes">
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
68
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
69 <param name="sequence" type="text" value="" help="" />
4
86c8fb3bb521 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 597c82a8fa756e68828ef201de7bb7e69d2c50bd
iuc
parents: 3
diff changeset
70 <param name="nb_mismatches" type="integer" value="2" min="0" help="If there is evidence for low-quality bases in the transposon tag (from FastQC, for instance), setting this to 1 or 2 may result in higher recovery of insertion sites. Higher than 2 is not advisable with the typical transposon tag lengths (10 - 12 bases) produced by TraDIS protocols, but may be appropriate with protocols that produce significantly longer transposon tags." />
0
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
71 <param name="tagdir" type="select" label="Direction of the transposon tag" help="" >
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
72 <option value="3" selected="true">3'</option>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
73 <option value="5">5bacteria_tradis.xml'</option>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
74 </param>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
75
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
76 </when>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
77 <when value="no">
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
78 </when>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
79 </conditional>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
80
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
81
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
82 </inputs>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
83
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
84 <outputs>
1
e8cb43bd2fe4 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 61c83ac999a040604915ff7cf92670b2635b967e"
iuc
parents: 0
diff changeset
85 <data format="txt" name="Statistics" from_work_dir="file.stats" label="${tool.name} on ${on_string} : Statistics" />
e8cb43bd2fe4 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 61c83ac999a040604915ff7cf92670b2635b967e"
iuc
parents: 0
diff changeset
86 <data name="Counts" format="tabular.gz" from_work_dir="./*.gz" label="${tool.name} on ${on_string} : Counts" />
e8cb43bd2fe4 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 61c83ac999a040604915ff7cf92670b2635b967e"
iuc
parents: 0
diff changeset
87 <data name="Aligned_reads" format="bam" from_work_dir="./*.bam" label="${tool.name} on ${on_string} : Mapped Reads" />
0
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
88 </outputs>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
89
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
90 <tests>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
91 <test>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
92 <param name="input_fastq" ftype="fastq" value="tiny.fastq.gz"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
93 <param name="input_ref" ftype="fasta" value="tiny_ref.fasta"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
94 <param name="map_options" value="default"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
95 <param name="min_quality" ftype="float" value="0"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
96 <param name="use" value="no"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
97 <param name="set" ftype="select" value="no"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
98 <output name="Statistics" file="file.stats" lines_diff="2" />
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
99 <output name="Counts" file="tiny.out.gz.CP009273.1_60_120.insert_site_plot.gz" compare="diff" decompress="true" lines_diff="0" />
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
100 </test>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
101 <test>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
102 <param name="input_fastq" ftype="fastq" value="tiny.fastq.gz"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
103 <param name="input_ref" ftype="fasta" value="tiny_ref.fasta"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
104 <param name="min_quality" ftype="integer" value="0"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
105 <param name="map_options" value="modify"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
106 <param name="min_percentage" ftype="float" value="0.5"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
107 <param name="duplicate_reads" ftype="boolean" value="-1"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
108 <param name="min_quality" ftype="float" value="20"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
109 <param name="use" value="no"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
110 <param name="set" ftype="select" value="yes"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
111 <param name="kmer_length" ftype="integer" value="10"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
112 <param name="step_size" ftype="integer" value="5"/>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
113 <output name="Statistics" file="file.stats" lines_diff="2" />
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
114 <output name="Counts" file="tiny_1.out.gz.CP009273.1_60_120.insert_site_plot.gz" compare="diff" decompress="true" lines_diff="0" />
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
115 </test>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
116
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
117 </tests>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
118 <help>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
119 <![CDATA[
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
120
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
121 **What is does**
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
122
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
123 Bio-TraDis provides software utilities for the processing, mapping, and analysis of transposon insertion sequencing data. The pipeline was designed with the data from the TraDIS sequencing protocol in mind, but should work with a variety of transposon insertion sequencing protocols as long as they produce data in the expected format.
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
124
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
125 -----
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
126
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
127 **Parameters**
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
128
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
129 The --smalt_r 0 and -m 0 options specify that we want to map reads with multiple best mappings to a random position and use these in our downstream analyses; by default these reads are left unmapped. Mapping and processing this library will take about 30 minutes to an hour on a typical desktop computer.
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
130
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
131 By default, the ​bacteria_tradis​ pipeline determines appropriate read mapping parameters automatically from the length of the first read in the fastq file. It should be noted that the default parameters have been tested using the optimized TraDIS protocol of Barquist ​et al​., 20XX in the hands of an experienced sequencing specialist; these will need to be tuned for other protocols, or for pilot runs, etc. There are various other scenarios in which it would be appropriate to reduce the stringency of these parameters: in the case that read trimming has been applied, if there are quality issues in the library, for certain types of studies (particularly gene essentiality studies as above), or if the quality of the reference genome is low (or of a different strain).
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
132
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
133
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
134 The *-mm* option specifies the number of mismatches allowed when matching the transposon tag; by default none are allowed. We sometimes observe one or two positions within the transposon tag that seem to have generally low quality. If there is evidence for low-quality bases in the transposon tag (from FastQC, for instance), setting this to 1 or 2 may result in higher recovery of insertion sites. Higher than 2 is not advisable with the typical transposon tag lengths (10 - 12 bases) produced by TraDIS protocols, but may be appropriate with protocols that produce significantly longer transposon tags.
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
135
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
136
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
137 The *-m* option sets the minimum mapping quality score to use an alignment in downstream analysis (e.g. plot files); defaults to 30. Multi-mapping reads have a quality score of 0 by definition, so this parameter needs to be set to 0 for these reads to be properly processed. Can be lowered without dramatically affecting results in most cases, particularly if *smalt_y* is set reasonably.
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
138
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
139
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
140 The other options specify parameters for the smalt mapper, which are discussed in more detail in the smalt manual (ftp.sanger.ac.uk/pub/resources/software/smalt/smalt-manual-0.7.4.pdf). We will discuss their effects on TraDIS mapping briefly here:
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
141
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
142 *-smalt_k*: length of kmers hashed; roughly, the minimum length of an exact match between a read and the genome needed to trigger an alignment attempt. Appropriate values are between ~10 and 20 for bacterial genomes depending on read length. Lower values lead to increased sensitivity at the expense of runtime.
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
143
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
144 *-smalt_s*: skipstep. Sampling step size, i.e. the distance between successive words that are hashed along the genomic reference sequence. With the option -s 1
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
145 every word is hashed, with -s 2 every second word, with -s 3 very third etc. Appropriate values are between 1 and ~15, but should be less than --smalt_k to ensure kmers overlap. Lower values lead to increased sensitivity at the expense of runtime.
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
146
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
147
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
148 *-smalt_y*: minimum percentage of identical bases between read and reference, defaults to .96 - 96% identity, or 4 mismatches allowed in a 100 base read. May be lowered to improve sensitivity in the case of low quality or short reads.
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
149
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
150
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
151 *-smalt_r*: specifies what to do with reads that map equally well in multiple locations. By default this is set to -1, meaning that multi-mapping reads are left unmapped. This is appropriate in studies comparing insertion frequency in the same library passaged through multiple conditions, as in this case a change in frequency of one repetitive gene could lead to many genes appearing to be selected artifactually. For studies of gene essentiality in a newly created library, this should be set to 0 (randomly assign a position) to avoid repetitive elements (particularly insertion sequences and the like) artificially appearing to be essential.
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
152
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
153 -----
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
154
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
155 **Output files**
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
156
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
157 On completion, bacteria tradis ​produces a number of files. These include:
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
158 **(input list name).stats**​ : Mapping statistics file. This is comma delimited, and includes one line for each library mapped along with a header. It can be easily opened in e.g. Excel or R.
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
159 **(library name.replicon_name).insert_site_plot.gz**​: Plot files, one for each replicon and library. These contain insertion counts on each strand for every nucleotide position in the replicon. They can be opened as “user plots” in the Artemis genome browser, and will be used for further analysis.
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
160 **(library name).mapped.bam** : BAM file containing mapped reads.
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
161
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
162 -----
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
163
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
164 **More information**
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
165
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
166 .. class:: infomark
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
167
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
168 Additional information about Bio-TraDis can be found at https://github.com/sanger-pathogens/Bio-Tradis
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
169 ]]>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
170 </help>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
171
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
172 <expand macro="citations" />
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
173
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
174 </tool>
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
175
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
176
bccf5ebe9b55 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/biotradis commit 1c0a0f88149bf8863a89c58bace81e070b3adb5a"
iuc
parents:
diff changeset
177