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1 """
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2 cwpair2.py
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3
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4 Takes a list of called peaks on both strands and produces a list of matched pairs and a list
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5 of unmatched orphans using a specified method for finding matched pairs. Methods for finding
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6 matched pairs are mode, closest, largest or all, where the analysis is run for each method
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7
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8 Input: list of one or more gff format files
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9
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10 Output: files produced for each input/mode combination:
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11 MP (matched_pair), D (details), O (orphans), P (frequency preview plot), F (frequency final plot),
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12 C (statistics graph), statistics.tabular
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13 """
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14
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15 import argparse
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16 import csv
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17 import cwpair2_util
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18
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19 if __name__ == '__main__':
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20 parser = argparse.ArgumentParser()
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21 parser.add_argument('--input', dest='inputs', action='append', nargs=2, help="Input datasets")
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22 parser.add_argument('--method', dest='method', default='mode', help='Method of finding match.')
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23 parser.add_argument('--up_distance', dest='up_distance', type=int, default=50, help='Distance upstream from a pair.')
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24 parser.add_argument('--down_distance', dest='down_distance', type=int, default=100, help='Distance downstream of a pair.')
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25 parser.add_argument('--binsize', dest='binsize', type=int, default=1, help='Width of bins for plots and mode.')
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26 parser.add_argument('--threshold_format', dest='threshold_format', help='Percentage to filter the 95th percentile.')
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27 parser.add_argument('--relative_threshold', dest='relative_threshold', type=float, default=0.0, help='Percentage to filter the 95th percentile.')
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28 parser.add_argument('--absolute_threshold', dest='absolute_threshold', type=float, default=0.0, help='Absolute value to filter.')
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29 parser.add_argument('--output_files', dest='output_files', default='matched_pair', help='Restrict output dataset collections.')
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30 parser.add_argument('--statistics_output', dest='statistics_output', help='Statistics output file.')
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31 args = parser.parse_args()
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32
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33 cwpair2_util.create_directories()
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34
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35 statistics = []
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36 if args.absolute_threshold > 0:
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37 threshold = args.absolute_threshold
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38 elif args.relative_threshold > 0:
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39 threshold = args.relative_threshold / 100.0
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40 else:
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41 threshold = 0
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42 for (dataset_path, hid) in args.inputs:
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43 stats = cwpair2_util.process_file(dataset_path,
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44 hid,
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45 args.method,
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46 threshold,
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47 args.up_distance,
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48 args.down_distance,
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49 args.binsize,
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50 args.output_files)
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51 statistics.extend(stats)
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52 # Accumulate statistics.
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53 by_file = {}
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54 for stats in statistics:
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55 # Skip "None" statistics from failed files
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56 if not stats:
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57 continue
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58 path = stats['stats_path']
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59 if path not in by_file:
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60 by_file[path] = []
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61 by_file[path].append(stats)
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62 # Write tabular statistics file.
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63 keys = ['fname', 'final_mode', 'preview_mode', 'perc95', 'paired', 'orphans']
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64 statistics_out = csv.writer(open(args.statistics_output, 'wt'), delimiter='\t')
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65 statistics_out.writerow(keys)
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66 for file_path, statistics in by_file.items():
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67 for stats in statistics:
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68 statistics_out.writerow([stats[key] for key in keys])
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