Mercurial > repos > fubar > rgrnastar_203e
changeset 35:e2dd4a5fad65 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar_240d commit 344140b8df53b8b7024618bb04594607a045c03a
author | iuc |
---|---|
date | Mon, 04 May 2015 22:46:43 -0400 |
parents | 99ea1fc51f4b |
children | |
files | rg_rnaStar.xml |
diffstat | 1 files changed, 19 insertions(+), 19 deletions(-) [+] |
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--- a/rg_rnaStar.xml Tue Apr 28 22:56:01 2015 -0400 +++ b/rg_rnaStar.xml Mon May 04 22:46:43 2015 -0400 @@ -4,6 +4,10 @@ <requirement type="package" version="2.4.0d">rnastar</requirement> <requirement type="package" version="0.1.19">samtools</requirement> </requirements> + <stdio> + <regex match=".*" source="both" level="warning" description="Some stderr/stdout text"/> + </stdio> + <command> ## ## Run STAR. @@ -24,9 +28,9 @@ #if str($refGenomeSource.genomeSource) == 'history': --genomeDir "tempstargenomedir" #else - --genomeDir "$refGenomeSource.index.fields.path" + --genomeDir "$refGenomeSource.index.fields.path" #end if - --readFilesIn $singlePaired.input1 + --readFilesIn $singlePaired.input1 #if str($singlePaired.sPaired) == "paired" $singlePaired.input2 #end if @@ -45,20 +49,16 @@ ## ## Convert aligned reads. - samtools view -Shb Aligned.out.sam | samtools sort - AlignedSorted 2>/dev/null + samtools view -Shb Aligned.out.sam | samtools sort - AlignedSorted 2>/dev/null ## Convert chimeric reads. #if str($params.settingsType) == "full" and $params.chim_segment_min > 0: - ; samtools view -Shb Chimeric.out.sam | samtools sort - ChimericSorted 2>/dev/null + ; samtools view -Shb Chimeric.out.sam | samtools sort - ChimericSorted 2>/dev/null #end if </command> - <stdio> - <regex match=".*" source="both" level="warning" description="Some stderr/stdout text"/> - </stdio> - <inputs> - <param name="jobName" type="text" size="120" value="rna-star run" label="Job narrative (added to output names)" + <param name="jobName" type="text" size="120" value="rna-star run" label="Job narrative (added to output names)" help="Only letters, numbers and underscores (_) will be retained in this field"> <sanitizer invalid_char=""> <valid initial="string.letters,string.digits"><add value="_" /> </valid> @@ -74,7 +74,7 @@ <param format="fastqsanger,fastq,fasta" name="input1" type="data" label="RNA-Seq FASTQ file" help="Nucleotide-space: Must have Sanger-scaled quality values with ASCII offset 33"/> </when> <when value="paired"> - <param format="fastqsanger,fastq,fasta" name="input1" type="data" label="RNA-Seq FASTQ file, forward reads" + <param format="fastqsanger,fastq,fasta" name="input1" type="data" label="RNA-Seq FASTQ file, forward reads" help="Nucleotide-space: Must have Sanger-scaled quality values with ASCII offset 33" /> <param format="fastqsanger,fastq,fasta" name="input2" type="data" label="RNA-Seq FASTQ file, reverse reads" help="Nucleotide-space: Must have Sanger-scaled quality values with ASCII offset 33" /> @@ -152,7 +152,7 @@ </conditional> </actions> </data> - <data format="bam" name="chimeric_reads" label="${jobName}_starmappedchim.bam" + <data format="bam" name="chimeric_reads" label="${jobName}_starmappedchim.bam" from_work_dir="ChimericSorted.bam"> <filter>(params['settingsType'] == 'full' and params['chim_segment_min'] > 0)</filter> <actions> @@ -173,7 +173,7 @@ </conditional> </actions> </data> - <data format="interval" name="splice_junctions" label="${jobName}_starsplicejunct.bed" + <data format="interval" name="splice_junctions" label="${jobName}_starsplicejunct.bed" from_work_dir="SJ.out.tab"> <actions> <conditional name="refGenomeSource.genomeSource"> @@ -193,7 +193,7 @@ </conditional> </actions> </data> - <data format="bam" name="mapped_reads" label="${jobName}_starmapped.bam" + <data format="bam" name="mapped_reads" label="${jobName}_starmapped.bam" from_work_dir="AlignedSorted.bam"> <actions> <conditional name="refGenomeSource.genomeSource"> @@ -287,8 +287,8 @@ filter out alignments that contain non-canonical unannotated junctions when using annotated splice junctions database. The annotated non- canonical junctions will be kept. - - + + **Attributions** Note that each component has its own license. Good luck with figuring out your obligations. @@ -299,12 +299,12 @@ "STAR: ultrafast universal RNA-seq aligner" A. Dobin et al, Bioinformatics 2012; doi: 10.1093/bioinformatics/bts635 -Galaxy_ (that's what you are using right now!) for gluing everything together +Galaxy_ (that's what you are using right now!) for gluing everything together -Most of the work for this wrapper XML is Jeremy Goecks' original STAR_ wrapper +Most of the work for this wrapper XML is Jeremy Goecks' original STAR_ wrapper -Minor tweaks to output names to suit our downstream purposes, toolshed automated dependencies -and odds and ends of other code and documentation comprising this tool was +Minor tweaks to output names to suit our downstream purposes, toolshed automated dependencies +and odds and ends of other code and documentation comprising this tool was written by Ross Lazarus and that part is licensed_ the same way as other rgenetics artefacts .. _STAR: https://bitbucket.org/jgoecks/jeremys-code/raw/fa1930a689b8e2f6b59cc1706e5ba0ed8ad357be/galaxy/tool-wrappers/star.xml