Mercurial > repos > erinija > dnp_subset_dinuc_profile
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"planemo upload commit 1a32efb8343938e8d49190003f251c78b5a58225-dirty"
author | erinija |
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date | Fri, 01 May 2020 12:11:00 +0000 |
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#!/bin/sh if test "$#" -ne 4; then echo "" echo " CALL " echo " sh dnp-smooth.sh smoothing-input.tabular winsize trim smoothed-output.tabular" echo "" echo " INPUT " echo " smoothing-input.tabular - dinucleotide frequency profiles-patterns to smooth" echo " winsize - size of averaging window, suggested optimal value =3" echo " trim - how many noisy points to remove from both ends of the profile suggested =4" echo "" echo " OUTPUT " echo " smoothed-output.tabular - original series smoothed " echo "" echo " DESCRIPRION" echo " Applies smoothing on dinucleotide profiles. Smoothing reduces noise and enhances" echo " a representation of the dinucleotide frequency profiles. Smoothing is performed by" echo " moving average with a chosen window size (optimal winsize=3). Smoothing script is based on" echo " a shell wrapper of the call to dnp-fourier tool which computes a periodogram" echo " of a series given as a numerical column and has parameters:" echo " dnp-fourier -f input -o output -n {normalization 0|1|2} -l length_of_smoothing_window -t {type_of_output 1|2|3}" echo " The parameters control a type of normalization and output as follows:" echo " Normalization" echo " 0 base normalization subtracts mean" echo " 1 linear normalization removes linear trand " echo " 2 quadratic normalization removes quadratic trend" echo " Output type" echo " 1 normalization outputs normalized original series" echo " 2 smoothing outputs smoothed original series" echo " 3 Fourier transform outputs periofogram" echo "" echo " Example of input table" echo " pos AA AC AG AT CA CC CG CT GA GC GG GT TA TC TG TT WW SS RR YY" echo " -73 0.05664 0.0657 0.06966 0.03644 0.08026 0.09484 0.0362 0.09086 0.07084 0.04032 0.07318 0.06466 0.03862 0.06838 0.05722 0.05602 0.18772 0.24454 0.27032 0.3101" echo " -72 0.0668 0.06476 0.0753 0.04282 0.07022 0.08034 0.03534 0.081 0.07222 0.03512 0.06774 0.0598 0.03934 0.07496 0.06628 0.06784 0.2168 0.21854 0.28206 0.30414" echo " -71 0.063 0.0621 0.07668 0.04316 0.06926 0.07264 0.03316 0.07992 0.07546 0.03498 0.07306 0.06406 0.04182 0.07374 0.06874 0.06812 0.2161 0.21384 0.2882 0.29442" echo " -70 0.0624 0.0643 0.07214 0.04424 0.0642 0.06998 0.03472 0.07718 0.0723 0.03982 0.07472 0.06818 0.04282 0.07674 0.06864 0.06754 0.217 0.21924 0.28156 0.29144" echo " -69 0.0622 0.06456 0.074 0.0426 0.0661 0.07114 0.03414 0.08016 0.0703 0.03786 0.07118 0.06754 0.0421 0.07712 0.06988 0.06904 0.21594 0.21432 0.27768 0.29746" echo " ..." echo "" echo " Output table is an original input table but smoothed by moving average with given window size." echo "" echo " REQUIREMENT" echo " dnp-fourier installed" echo " conda install -c bioconda dnp-fourier" echo "" exit 1 fi # input file name name=$1 swindow=$2 trim=$3 out=$4 call=dnp-fourier ## get the nucleotides from the header dinucleotides=`head -n1 ${name} | sed 's/pos//'` ## save the position information posi=`awk '{ for (i=1; i<=NF; i++) if($i=="pos") print i; exit}' ${name}` awk -v k=${posi} '{print $k}' $name | grep -v pos > posfile for di in ${dinucleotides} do # column number of dinucleotide i1=`awk -v name=$di '{ for (i=1; i<=NF; i++) if($i==name) print i; exit}' ${name}` awk -v k=${i1} '{print $k}' $name | grep -v ${di} > temp.${di} # add centered sequence position paste posfile temp.${di} | tr "\t" " " > smoothprep # perform smoothing ${call} -f smoothprep -t 2 -l ${swindow} -o cps.${di} # echo ${di} > cp.${di} cat cps.${di} | tail -n +$(($trim+1)) | head -n -$(($trim+1)) > temp.${di} echo "pos" > positions cat temp.${di} | awk '{print $1}' >> positions echo ${di} > cp.${di} cat temp.${di} | awk '{print $2}' >> cp.${di} done #echo "pos" ${dinucleotides} | tr " " "\t" > ${out} paste positions cp.* > ${out} rm temp.* cp.* cps.* posfile smoothprep positions