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author | erinija |
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date | Fri, 01 May 2020 12:08:23 +0000 |
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#!/bin/sh if test "$#" -ne 5; then echo "" echo " CALL " echo " sh dnp-fourier-transform.sh difreq.profiles.tabular difreq.periodogram.tabular normalization winsize trim" echo "" echo " INPUT " echo " difreq.profiles.tabular - dinucleotide frequency profiles-patterns" echo " normalization - type of normalization, suggested is quadratic =2" echo " winsize - size of averaging window, suggested optimal value =3" echo " trim - how many noisy points to remove from both ends of the profile suggested =4" echo "" echo " OUTPUT " echo " difreq.periodogram.tabular - periodogram " echo "" echo " DESCRIPRION" echo " This is a shell wrapper of the call to dnp-fourier which computes a periodogram " echo " of a series given as a numerical column and has parameters:" echo " dnp-fourier -f input -o output -n {normalization 0|1|2} -l length_of_smoothing_window -t {type_of_output 1|2|3}" echo "" echo " The parameters control a type of normalization and output:" echo " Normalization" echo " 0 base normalization subtracts mean" echo " 1 linear normalization removes linear trand " echo " 2 quadratic normalization removes quadratic trend" echo " Output type" echo " 1 normalization outputs normalized original series" echo " 2 smoothing outputs smoothed original series" echo " 3 Fourier transform outputs periofogram" echo "" echo " Fourier transform has to be applied on symmetrized, nonsmoothed data, since it performs" echo " smoothing internally. Dinucleotide frequency profiles usually have a quadratic gradient," echo " therefore a quadratic normalization is used by default. Fourier transform is applied to every " echo " column of the input table. In the output the first column contains period as a number of base pairs." echo "" echo " Example of input table" echo " pos AA AC AG AT CA CC CG CT GA GC GG GT TA TC TG TT WW SS RR YY" echo " -73 0.05664 0.0657 0.06966 0.03644 0.08026 0.09484 0.0362 0.09086 0.07084 0.04032 0.07318 0.06466 0.03862 0.06838 0.05722 0.05602 0.18772 0.24454 0.27032 0.3101" echo " -72 0.0668 0.06476 0.0753 0.04282 0.07022 0.08034 0.03534 0.081 0.07222 0.03512 0.06774 0.0598 0.03934 0.07496 0.06628 0.06784 0.2168 0.21854 0.28206 0.30414" echo " -71 0.063 0.0621 0.07668 0.04316 0.06926 0.07264 0.03316 0.07992 0.07546 0.03498 0.07306 0.06406 0.04182 0.07374 0.06874 0.06812 0.2161 0.21384 0.2882 0.29442" echo " -70 0.0624 0.0643 0.07214 0.04424 0.0642 0.06998 0.03472 0.07718 0.0723 0.03982 0.07472 0.06818 0.04282 0.07674 0.06864 0.06754 0.217 0.21924 0.28156 0.29144" echo " -69 0.0622 0.06456 0.074 0.0426 0.0661 0.07114 0.03414 0.08016 0.0703 0.03786 0.07118 0.06754 0.0421 0.07712 0.06988 0.06904 0.21594 0.21432 0.27768 0.29746" echo " ..." echo "" echo " Example of output" echo " period AA AC AG ..." echo " 2.100000 0.055962 0.061351 0.059462 ..." echo " 2.200000 0.031410 0.027762 0.030298 ..." echo " ..." echo "" echo " REQUIREMENT" echo " dnp-fourier installed" echo " conda install -c bioconda dnp-fourier" echo "" exit 1 fi # column comes with the header which we have to remove input=$1 output=$2 #normalization=2 normalization=$3 #smoothingw=3 smoothingw=$4 #trim=2 trim=$5 trim1=$((trim+1)) outputtype=3 call=dnp-fourier dinucleotides=`head -n1 ${input} | sed 's/pos//'` for di in ${dinucleotides} do # column number of dinucleotide i1=`awk -v name=$di '{ for (i=1; i<=NF; i++) if($i==name) print i; exit}' ${input}` awk -v k=${i1} '{print $k}' $input | grep -v ${di} | tail -n +${trim1} | head -n -${trim}| cat -n > temp.${di} # Submit the call and parameters ${call} -f temp.${di} -o ft_output.${di} -n ${normalization} -l ${smoothingw} -t ${outputtype} echo "period" > period awk '{print $1}' ft_output.${di} >> period echo ${di} > ft.${di} awk '{print $2}' ft_output.${di} >> ft.${di} done paste period ft.* > ${output} rm temp.* ft.* period ft_output.*