changeset 4:92921dfea0b5 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/annotateviz commit 230f5fe14cc469e56626201a5c377686976d81fc
author eduardo
date Sat, 24 Jun 2017 14:55:25 -0400
parents 3302f18f9e16
children 9cc97d18dff1
files annotateviz.py annotateviz.xml prediction.json static/js/editgff.js static/js/gff_app.js static/trinotateviz.html test-data/gff.json
diffstat 7 files changed, 120 insertions(+), 11 deletions(-) [+]
line wrap: on
line diff
--- a/annotateviz.py	Sat Jun 17 15:10:53 2017 -0400
+++ b/annotateviz.py	Sat Jun 24 14:55:25 2017 -0400
@@ -18,7 +18,8 @@
                 argv.append("-h")
         parser = argparse.ArgumentParser(formatter_class=argparse.RawDescriptionHelpFormatter, description=__doc__)
         parser.add_argument('-d','--database', help="gffutils sqlite database")
-        parser.add_argument('-j','--json', help="report in json format")
+        parser.add_argument('-j','--json', help="annotations in json format")
+        parser.add_argument('-p','--pred', help="predictions in json")
         args = parser.parse_args(argv)
         gffutils.constants.always_return_list = False
         db = gffutils.interface.FeatureDB(args.database)
@@ -29,5 +30,34 @@
         add_matches(db.features_of_type("PFAM"), prediction_list)
         fout=open(args.json, 'w')
         json.dump(prediction_list,fout)
+        # create json for protein predictions
+        array=[]
+        for f in db.features_of_type("gene"):
+            dict = {}
+            dict['seqid'] = f.seqid
+            dict['source'] = f.source
+            dict['featuretype'] = f.featuretype
+            dict['start'] = f.start
+            dict['end'] = f.end
+            dict['score'] = f.score
+            dict['strand'] = f.strand
+            dict['frame'] = f.frame
+            dict['attributes']=f.attributes.__dict__["_d"]
+            array.append(dict)
+            for c in db.children(f):
+                dict = {}
+                dict['seqid'] = c.seqid
+                dict['source'] = c.source
+                dict['featuretype'] = c.featuretype
+                dict['start'] = c.start
+                dict['end'] = c.end
+                dict['score'] = c.score
+                dict['strand'] = c.strand
+                dict['frame'] = c.frame
+                dict['attributes'] = c.attributes.__dict__["_d"]
+                array.append(dict)
+        fout2=open(args.pred,'w')
+        json.dump(array,fout2)
+
 if __name__ == "__main__":
         main(sys.argv[1:],sys.stdout)
--- a/annotateviz.xml	Sat Jun 17 15:10:53 2017 -0400
+++ b/annotateviz.xml	Sat Jun 24 14:55:25 2017 -0400
@@ -14,9 +14,10 @@
 --outdir $output.files_path
 $trackxml &&
 ##  $output.files_path/index.html $output;
-python  $__tool_directory__/annotateviz.py -d $db -j ${output.files_path}/trans.json &&
+python  $__tool_directory__/annotateviz.py -d $db -j ${output.files_path}/trans.json -p ${output.files_path}/gff.json &&
 sed "s@input_db@${__app__.security.encode_id($db.id)}@" $__tool_directory__/static/trinotateviz.html > $output &&
 cp $__tool_directory__/static/js/gff_app.js ${output.files_path} &&
+cp $__tool_directory__/static/js/edit.js ${output.files_path} &&
 cp $__tool_directory__/static/css/main.css ${output.files_path};
 ]]></command>
   <configfiles>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/prediction.json	Sat Jun 24 14:55:25 2017 -0400
@@ -0,0 +1,1 @@
+[{"featuretype": "gene", "end": 344, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5404_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN5404_c0_g1_i1|g.6938"], "Name": ["ORF%20TRINITY_DN5404_c0_g1_i1%7Cg.6938%20TRINITY_DN5404_c0_g1_i1%7Cm.6938%20type%3Ainternal%20len%3A115%20%28-%29"]}, "strand": "-"}, {"featuretype": "mRNA", "end": 344, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5404_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN5404_c0_g1_i1|m.6938"], "Parent": ["TRINITY_DN5404_c0_g1_i1|g.6938"], "Name": ["ORF%20TRINITY_DN5404_c0_g1_i1%7Cg.6938%20TRINITY_DN5404_c0_g1_i1%7Cm.6938%20type%3Ainternal%20len%3A115%20%28-%29"]}, "strand": "-"}, {"featuretype": "five_prime_UTR", "end": 344, "score": ".", "start": 343, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5404_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN5404_c0_g1_i1|m.6938.utr5p1"], "Parent": ["TRINITY_DN5404_c0_g1_i1|m.6938"]}, "strand": "-"}, {"featuretype": "exon", "end": 344, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5404_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN5404_c0_g1_i1|m.6938.exon1"], "Parent": ["TRINITY_DN5404_c0_g1_i1|m.6938"]}, "strand": "-"}, {"featuretype": "CDS", "end": 342, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5404_c0_g1_i1", "attributes": {"ID": ["cds.TRINITY_DN5404_c0_g1_i1|m.6938"], "Parent": ["TRINITY_DN5404_c0_g1_i1|m.6938"]}, "strand": "-"}, {"featuretype": "gene", "end": 605, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5409_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN5409_c0_g1_i1|g.6942"], "Name": ["ORF%20TRINITY_DN5409_c0_g1_i1%7Cg.6942%20TRINITY_DN5409_c0_g1_i1%7Cm.6942%20type%3Ainternal%20len%3A202%20%28-%29"]}, "strand": "-"}, {"featuretype": "mRNA", "end": 605, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5409_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN5409_c0_g1_i1|m.6942"], "Parent": ["TRINITY_DN5409_c0_g1_i1|g.6942"], "Name": ["ORF%20TRINITY_DN5409_c0_g1_i1%7Cg.6942%20TRINITY_DN5409_c0_g1_i1%7Cm.6942%20type%3Ainternal%20len%3A202%20%28-%29"]}, "strand": "-"}, {"featuretype": "five_prime_UTR", "end": 605, "score": ".", "start": 604, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5409_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN5409_c0_g1_i1|m.6942.utr5p1"], "Parent": ["TRINITY_DN5409_c0_g1_i1|m.6942"]}, "strand": "-"}, {"featuretype": "exon", "end": 605, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5409_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN5409_c0_g1_i1|m.6942.exon1"], "Parent": ["TRINITY_DN5409_c0_g1_i1|m.6942"]}, "strand": "-"}, {"featuretype": "CDS", "end": 603, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5409_c0_g1_i1", "attributes": {"ID": ["cds.TRINITY_DN5409_c0_g1_i1|m.6942"], "Parent": ["TRINITY_DN5409_c0_g1_i1|m.6942"]}, "strand": "-"}, {"featuretype": "gene", "end": 1934, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN540_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN540_c0_g1_i1|g.650"], "Name": ["ORF%20TRINITY_DN540_c0_g1_i1%7Cg.650%20TRINITY_DN540_c0_g1_i1%7Cm.650%20type%3Acomplete%20len%3A407%20%28%2B%29"]}, "strand": "+"}, {"featuretype": "mRNA", "end": 1934, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN540_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN540_c0_g1_i1|m.650"], "Parent": ["TRINITY_DN540_c0_g1_i1|g.650"], "Name": ["ORF%20TRINITY_DN540_c0_g1_i1%7Cg.650%20TRINITY_DN540_c0_g1_i1%7Cm.650%20type%3Acomplete%20len%3A407%20%28%2B%29"]}, "strand": "+"}]
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/static/js/editgff.js	Sat Jun 24 14:55:25 2017 -0400
@@ -0,0 +1,68 @@
+var jsonobj=[]
+ $.ajax({url: "gff.json", success: function(result){
+        jsonobj = result;
+    }});
+
+
+function merge_prediction() {
+    var lpart = document.getElementById("lpart").value;
+    var rpart = document.getElementById("rpart").value;
+    edit_annot(lpart,rpart);
+}
+
+
+
+
+function edit_annot(lpart,rpart) {
+     for (var i = jsonobj.length -1; i >= 0 ; i--) {
+        if (jsonobj[i].attributes.hasOwnProperty("Parent")) {
+            if (jsonobj[i].attributes.Parent[0] === lpart){
+                if (jsonobj[i].featuretype == "three_prime_UTR") {
+                    jsonobj.splice(i,1);
+                    console.log("removed lpart three_prime_UTR");
+                }
+                else {
+                    jsonobj[i].attributes.Parent[0] = '.'.join(lpart,1);
+                }
+            }
+            if (jsonobj[i].attributes.Parent[0] === rpart){
+                if (jsonobj[i].featuretype == "five_prime_UTR") {
+                    jsonobj.splice(i,1);
+                    console.log("removed rpart five_prime_UTR");
+                }
+                else {
+                    jsonobj[i].attributes.Parent[0] = '.'.join(lpart,1);
+                }
+            }
+        }
+
+    };
+}
+
+function export_gff() {
+    var text="";
+    var line="";
+    for (var i=0;i<jsonobj.length;i++){
+        var attributes="";
+        for (var attribute in jsonobj[i].attributes){
+            attributes=attribute+'='+jsonobj[i].attributes[attribute][0]+';';
+        }
+        line = jsonobj[i].seqid+'\t'+
+        jsonobj[i].source+'\t'+
+        jsonobj[i].featuretype+'\t'+
+        jsonobj[i].start+'\t'+
+        jsonobj[i].end+'\t'+
+        jsonobj[i].score+'\t'+
+        jsonobj[i].strand+'\t'+
+        jsonobj[i].frame+'\t'+
+        attributes;
+        text=text+line+'\'';
+    }
+    var gfffile = new Blob([text], {type: "text/plain;charset=utf-8"});
+    var blob = new Blob([text], {type: "text/plain;charset=utf-8"});
+    var link = document.createElement('a');
+    link.setAttribute('href', window.URL.createObjectURL(blob));
+    link.setAttribute('download', "gff_export");
+    link.click();
+}
+
--- a/static/js/gff_app.js	Sat Jun 17 15:10:53 2017 -0400
+++ b/static/js/gff_app.js	Sat Jun 24 14:55:25 2017 -0400
@@ -17,20 +17,18 @@
     //enableHorizontalScrollbar: 0,
     //enableVerticalScrollbar: 1,
     columnDefs: [
-      { name: 'contig', grouping: { groupPriority: 0 }, sort: { priority: 0, direction: 'desc' }, width: '25%', cellTemplate: '<div><div ng-if="!col.grouping || col.grouping.groupPriority === undefined || col.grouping.groupPriority === null || ( row.groupHeader && col.grouping.groupPriority === row.treeLevel )" class="ui-grid-cell-contents" title="TOOLTIP">{{COL_FIELD CUSTOM_FILTERS}}</div></div>' },
+      { name: 'Contig', grouping: { groupPriority: 0 }, sort: { priority: 0, direction: 'desc' }, width: '25%', cellTemplate: '<div><div ng-if="!col.grouping || col.grouping.groupPriority === undefined || col.grouping.groupPriority === null || ( row.groupHeader && col.grouping.groupPriority === row.treeLevel )" class="ui-grid-cell-contents" title="TOOLTIP">{{COL_FIELD CUSTOM_FILTERS}}</div></div>' },
       { name: 'mRNA', grouping: { groupPriority: 1 }, sort: { priority: 1,direction: 'desc' }, width: '25%', cellTemplate: '<div><div ng-if="!col.grouping || col.grouping.groupPriority === undefined || col.grouping.groupPriority === null || ( row.groupHeader && col.grouping.groupPriority === row.treeLevel )" class="ui-grid-cell-contents" title="TOOLTIP">{{COL_FIELD CUSTOM_FILTERS}}</div></div>' },
-      { name: 'prediction', width: '25%' },
-      { name: 'source', width: '10%' },
-      { name: 'score', width: '5%'},
+      { name: 'Prediction', width: '25%' },
+      { name: 'Source', width: '10%' },
+      { name: 'Score', width: '5%'},
     ],
     onRegisterApi: function( gridApi ) {
       $scope.gridApi = gridApi;
     }
   };
 
-    //var  baseurl= '/api/datasets/'+hdaId+'?provider=sqlite-dict&data_type=raw_data&query=';
-    //var  select="select%20seqid,seqid,source,featuretype,start,end,score,strand,frame,attributes%20from%20features";
-    //var url= baseurl + select;
+
     $http.get('trans.json')
      .success (function(response){
          $scope.gridOptions.data = response;
--- a/static/trinotateviz.html	Sat Jun 17 15:10:53 2017 -0400
+++ b/static/trinotateviz.html	Sat Jun 24 14:55:25 2017 -0400
@@ -8,9 +8,12 @@
     <script src="http://ui-grid.info/docs/grunt-scripts/pdfmake.js"></script>
     <script src="http://ui-grid.info/docs/grunt-scripts/vfs_fonts.js"></script>
     <script src="https://cdn.rawgit.com/angular-ui/bower-ui-grid/master/ui-grid.min.js"></script>
+   <script src="https://cdnjs.cloudflare.com/ajax/libs/jquery/3.1.0/jquery.min.js"></script>
+    <script src="editgff.js"></script>
+
     <link rel="stylesheet" href="https://cdn.rawgit.com/angular-ui/bower-ui-grid/master/ui-grid.min.css" type="text/css">
-      <link rel="stylesheet" href="main.css" type="text/css">
-      <link rel="stylesheet" type="text/css" href="css/genome.css">
+    <link rel="stylesheet" href="main.css" type="text/css">
+    <link rel="stylesheet" type="text/css" href="css/genome.css">
   </head>
   <body>
 
@@ -18,6 +21,12 @@
 
   <div ui-grid="gridOptions" ui-grid-pagination ui-grid-grouping ui-grid-exporter  class="grid"></div>
 </div>
+<div><br>
+left part: <input type="text" name="lpart" id="lpart">
+right part: <input type="text" name="rpart" id="rpart">
+<input type="button" value="Submit" onClick="merge_prediction() ></input>
+<input type="button" value="export" onClick="export_gff() ></input>
+<br></div>
 
     <div id="GenomeBrowser" style="height: 100%; width: 100%; padding: 0; border: 0;"></div>
     <div style="display: none">JBrowseDefaultMainPage</div>
@@ -123,4 +132,5 @@
  </script>
     <script src="gff_app.js"></script>
   </body>
+
 </html>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/gff.json	Sat Jun 24 14:55:25 2017 -0400
@@ -0,0 +1,1 @@
+[{"featuretype": "gene", "end": 344, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5404_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN5404_c0_g1_i1|g.6938"], "Name": ["ORF%20TRINITY_DN5404_c0_g1_i1%7Cg.6938%20TRINITY_DN5404_c0_g1_i1%7Cm.6938%20type%3Ainternal%20len%3A115%20%28-%29"]}, "strand": "-"}, {"featuretype": "mRNA", "end": 344, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5404_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN5404_c0_g1_i1|m.6938"], "Parent": ["TRINITY_DN5404_c0_g1_i1|g.6938"], "Name": ["ORF%20TRINITY_DN5404_c0_g1_i1%7Cg.6938%20TRINITY_DN5404_c0_g1_i1%7Cm.6938%20type%3Ainternal%20len%3A115%20%28-%29"]}, "strand": "-"}, {"featuretype": "five_prime_UTR", "end": 344, "score": ".", "start": 343, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5404_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN5404_c0_g1_i1|m.6938.utr5p1"], "Parent": ["TRINITY_DN5404_c0_g1_i1|m.6938"]}, "strand": "-"}, {"featuretype": "exon", "end": 344, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5404_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN5404_c0_g1_i1|m.6938.exon1"], "Parent": ["TRINITY_DN5404_c0_g1_i1|m.6938"]}, "strand": "-"}, {"featuretype": "CDS", "end": 342, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5404_c0_g1_i1", "attributes": {"ID": ["cds.TRINITY_DN5404_c0_g1_i1|m.6938"], "Parent": ["TRINITY_DN5404_c0_g1_i1|m.6938"]}, "strand": "-"}, {"featuretype": "gene", "end": 605, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5409_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN5409_c0_g1_i1|g.6942"], "Name": ["ORF%20TRINITY_DN5409_c0_g1_i1%7Cg.6942%20TRINITY_DN5409_c0_g1_i1%7Cm.6942%20type%3Ainternal%20len%3A202%20%28-%29"]}, "strand": "-"}, {"featuretype": "mRNA", "end": 605, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5409_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN5409_c0_g1_i1|m.6942"], "Parent": ["TRINITY_DN5409_c0_g1_i1|g.6942"], "Name": ["ORF%20TRINITY_DN5409_c0_g1_i1%7Cg.6942%20TRINITY_DN5409_c0_g1_i1%7Cm.6942%20type%3Ainternal%20len%3A202%20%28-%29"]}, "strand": "-"}, {"featuretype": "five_prime_UTR", "end": 605, "score": ".", "start": 604, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5409_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN5409_c0_g1_i1|m.6942.utr5p1"], "Parent": ["TRINITY_DN5409_c0_g1_i1|m.6942"]}, "strand": "-"}, {"featuretype": "exon", "end": 605, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5409_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN5409_c0_g1_i1|m.6942.exon1"], "Parent": ["TRINITY_DN5409_c0_g1_i1|m.6942"]}, "strand": "-"}, {"featuretype": "CDS", "end": 603, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN5409_c0_g1_i1", "attributes": {"ID": ["cds.TRINITY_DN5409_c0_g1_i1|m.6942"], "Parent": ["TRINITY_DN5409_c0_g1_i1|m.6942"]}, "strand": "-"}, {"featuretype": "gene", "end": 1934, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN540_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN540_c0_g1_i1|g.650"], "Name": ["ORF%20TRINITY_DN540_c0_g1_i1%7Cg.650%20TRINITY_DN540_c0_g1_i1%7Cm.650%20type%3Acomplete%20len%3A407%20%28%2B%29"]}, "strand": "+"}, {"featuretype": "mRNA", "end": 1934, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN540_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN540_c0_g1_i1|m.650"], "Parent": ["TRINITY_DN540_c0_g1_i1|g.650"], "Name": ["ORF%20TRINITY_DN540_c0_g1_i1%7Cg.650%20TRINITY_DN540_c0_g1_i1%7Cm.650%20type%3Acomplete%20len%3A407%20%28%2B%29"]}, "strand": "+"}, {"featuretype": "five_prime_UTR", "end": 349, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN540_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN540_c0_g1_i1|m.650.utr5p1"], "Parent": ["TRINITY_DN540_c0_g1_i1|m.650"]}, "strand": "+"}, {"featuretype": "exon", "end": 1934, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN540_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN540_c0_g1_i1|m.650.exon1"], "Parent": ["TRINITY_DN540_c0_g1_i1|m.650"]}, "strand": "+"}, {"featuretype": "CDS", "end": 1570, "score": ".", "start": 350, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN540_c0_g1_i1", "attributes": {"ID": ["cds.TRINITY_DN540_c0_g1_i1|m.650"], "Parent": ["TRINITY_DN540_c0_g1_i1|m.650"]}, "strand": "+"}, {"featuretype": "three_prime_UTR", "end": 1934, "score": ".", "start": 1571, "frame": ".", "source": "transdecoder", "seqid": "TRINITY_DN540_c0_g1_i1", "attributes": {"ID": ["TRINITY_DN540_c0_g1_i1|m.650.utr3p1"], "Parent": ["TRINITY_DN540_c0_g1_i1|m.650"]}, "strand": "+"}, {"featuretype": "gene", "end": 2211, "score": ".", "start": 1, "frame": ".", "source": "transdecoder", 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