Mercurial > repos > ebi-gxa > _hca_matrix_downloader
changeset 1:90fac5c7e0b7 draft default tip
Deleted selected files
author | ebi-gxa |
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date | Tue, 02 Jul 2019 03:32:15 -0400 |
parents | dae1539a77fa |
children | |
files | matrix-service.xml |
diffstat | 1 files changed, 0 insertions(+), 108 deletions(-) [+] |
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--- a/matrix-service.xml Mon Jul 01 19:03:27 2019 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,108 +0,0 @@ -<?xml version="1.0" encoding="utf-8"?> -<tool id="_hca_matrix_downloader" name="Human Cell Atlas Matrix Downloader" version="v0.0.2+galaxy0"> - <description>retrieves expression matrices and metadata from the Human Cell Atlas.</description> - <requirements> - <requirement type="package" version="0.0.2">hca-matrix-downloader</requirement> - </requirements> - <command detect_errors="exit_code"><![CDATA[ - -hca-matrix-downloader -p '${project}' -f mtx -o out -f '${matrix_format}' - -#if $matrix_format == "mtx": - && hca-mtx-to-10x out.mtx . - && gunzip -c out.mtx/cells.tsv.gz > exp_design.tsv -#end if - -]]></command> - - <inputs> - <param name="project" type="text" value="Single cell transcriptome analysis of human pancreas" label="Human Cell Atlas project name/label/UUID" help="HCA project identifier, can be project title, project label or project UUID."/> - <param name="matrix_format" type="select" label="Choose the format of matrix to download" help="Matrix Market or Loom"> - <option value="mtx" selected="true">Matrix Market</option> - <option value="loom">Loom</option> - </param> - </inputs> - - <outputs> - <data name="matrix_mtx" format="txt" from_work_dir="matrix.mtx" label="${tool.name} on ${on_string} matrix.mtx"> - <filter>matrix_format=="mtx"</filter> - </data> - <data name="genes_tsv" format="tsv" from_work_dir="genes.tsv" label="${tool.name} on ${on_string} genes.tsv"> - <filter>matrix_format=="mtx"</filter> - </data> - <data name="barcode_tsv" format="tsv" from_work_dir="barcodes.tsv" label="${tool.name} on ${on_string} barcodes.tsv"> - <filter>matrix_format=="mtx"</filter> - </data> - <data name="cells_meta_tsv" format="tsv" from_work_dir="exp_design.tsv" label="${tool.name} on ${on_string} exp_design.tsv"> - <filter>matrix_format=="mtx"</filter> - </data> - <data name="matrix_loom" format="h5" from_work_dir="out.loom" label="${tool.name} on ${on_string} matrix.loom"> - <filter>matrix_format=="loom"</filter> - </data> - </outputs> - - <tests> - <test> - <param name="project" value="Single cell transcriptome analysis of human pancreas"/> - <param name="matrix_format" value="mtx"/> - <output name="matrix_mtx" file="matrix.mtx" ftype="txt"/> - <output name="genes_tsv" file="genes.tsv" ftype="tsv"/> - <output name="barcode_tsv" file="barcodes.tsv" ftype="tsv"/> - <output name="cells_meta_tsv" file="exp_design.tsv" ftype="tsv"/> - </test> - </tests> - - <help><![CDATA[ -=========================================================================== -Down expression matrix from HCA projects using HCA DCP's matrix service API -=========================================================================== - -The data retrieval tool presented here allows the user to retrieve expression matrices -and metadata for any public experiment available at Human Cell Atlas data portal. - -To use it, simply set the name, or label, or ID for the desired project, which can be -found at the HCA data browser (https://prod.data.humancellatlas.org/explore/projects), -and select the desired matrix format (Matrix Market or Loom). - -Outputs will be: - -- *When "Matrix Market" is seleted, outputs are in 10X-compatible Matrix Market format:* - - 1. **Matrix (txt):** - - Contains the expression values for genes (rows) and cells (columns) in raw counts. This - text file is formatted as a Matrix Market file, and as such it is accompanied by separate - files for the gene identifiers and the cells identifiers. - - 2. **Genes (tsv):** - - Identifiers (column repeated) for the genes present in the matrix of expression, - in the same order as the matrix rows. - - 3. **Barcodes (tsv):** - - Identifiers for the cells of the data matrix. The file is ordered to match the columns - of the matrix. - - 4. **Experiment Design file (tsv):** - - Contains metadata for the different cells of the experiment. - -- *When "Loom" is selected, output is a single Loom HDF5 file:* - - 1. **Loom (h5):** - - Contains expression values for genes (rows) and cells (columns) in raw counts, cell - metadata table and gene metadata table, in a single HDF5 file with specification defined - in http://linnarssonlab.org/loompy/format/index.html. - -**Version history** - - 0.0.2+galaxy0: Initial contribution. Ni Huang and Pablo Moreno, Teichmann Lab at Wellcome Sanger Institute and - Expression Atlas team https://www.ebi.ac.uk/gxa/home at EMBL-EBI https://www.ebi.ac.uk/. - -]]></help> - <citations> - <citation type="doi">10.7554/eLife.27041</citation> - </citations> -</tool>