Mercurial > repos > devteam > samtools_phase
changeset 3:c21cb56fbbbc draft
planemo upload commit 33927a87ba2eee9bf0ecdd376a66241b17b3d734
author | devteam |
---|---|
date | Tue, 13 Oct 2015 12:55:05 -0400 |
parents | 2656247b5253 |
children | ee06ec9cf0a6 |
files | macros.xml samtools_phase.xml tool_dependencies.xml |
diffstat | 3 files changed, 81 insertions(+), 19 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Tue Oct 13 12:55:05 2015 -0400 @@ -0,0 +1,70 @@ +<macros> + <xml name="requirements"> + <requirements> + <requirement type="package" version="1.2">samtools</requirement> + <yield/> + </requirements> + </xml> + <xml name="citations"> + <citations> + <citation type="bibtex"> + @misc{SAM_def, + title={Definition of SAM/BAM format}, + url = {https://samtools.github.io/hts-specs/SAMv1.pdf},} + </citation> + <citation type="doi">10.1093/bioinformatics/btp352</citation> + <citation type="doi">10.1093/bioinformatics/btr076</citation> + <citation type="doi">10.1093/bioinformatics/btr509</citation> + <citation type="bibtex"> + @misc{Danecek_et_al, + Author={Danecek, P., Schiffels, S., Durbin, R.}, + title={Multiallelic calling model in bcftools (-m)}, + url = {http://samtools.github.io/bcftools/call-m.pdf},} + </citation> + <citation type="bibtex"> + @misc{Durbin_VCQC, + Author={Durbin, R.}, + title={Segregation based metric for variant call QC}, + url = {http://samtools.github.io/bcftools/rd-SegBias.pdf},} + </citation> + <citation type="bibtex"> + @misc{Li_SamMath, + Author={Li, H.}, + title={Mathematical Notes on SAMtools Algorithms}, + url = {http://www.broadinstitute.org/gatk/media/docs/Samtools.pdf},} + </citation> + <citation type="bibtex"> + @misc{SamTools_github, + title={SAMTools GitHub page}, + url = {https://github.com/samtools/samtools},} + </citation> + </citations> + </xml> + <xml name="version_command"> + <version_command>samtools --version | head -n 1 | awk '{ print $2 }'</version_command> + </xml> + <xml name="stdio"> + <stdio> + <exit_code range="1:" level="fatal" description="Error" /> + </stdio> + </xml> + <token name="@no-chrom-options@"> +----- + +.. class:: warningmark + +**No options available? How to re-detect metadata** + +If you see a "No options available" within the "**Select references (chromosomes and contigs) you would like to restrict bam to**" drop down, you need to re-detect metadata for the dataset you are trying to process. To do this follow these steps: + +1. Click on the **pencil** icon adjacent to the dataset in the history +2. A new menu will appear in the center pane of the interface +3. Click **Datatype** tab +4. Set **New Type** to **BAM** +5. Click **Save** + +The medatada will be re-detected and you will be able to see the list of reference sequences in the "**Select references (chromosomes and contigs) you would like to restrict bam to**" drop-down. + + </token> + +</macros>
--- a/samtools_phase.xml Fri Sep 26 15:20:35 2014 -0400 +++ b/samtools_phase.xml Tue Oct 13 12:55:05 2015 -0400 @@ -1,8 +1,11 @@ -<tool id="samtools_phase" name="Call and phase" version="1.0.2"> +<tool id="samtools_phase" name="Call and phase" version="2.0"> <description>heterozygous SNPs</description> - <requirements> - <requirement type="package" version="1.1">samtools</requirement> - </requirements> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements"></expand> + <expand macro="stdio"></expand> + <expand macro="version_command"></expand> <command>samtools phase -b "phase_wrapper" #if str($option_set.option_sets) == 'advanced': ${option_set.ignore_chimeras} @@ -16,9 +19,6 @@ #end if "$input_bam" > "$phase_sets" </command> - <stdio> - <exit_code range="1:" level="fatal" description="Error" /> - </stdio> <inputs> <param format="bam" name="input_bam" type="data" label="Select dataset to phase"/> <conditional name="option_set"> @@ -125,14 +125,6 @@ - Drop reads with ambiguous phase - *off* ------- - -**Citation** - -For the underlying tool, please cite `Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R; 1000 Genome Project Data Processing Subgroup. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009 Aug 15;25(16):2078-9. <http://www.ncbi.nlm.nih.gov/pubmed/19505943>`_ - - -If you use this tool in Galaxy, please cite Blankenberg D, et al. *In preparation.* - - </help> + </help> + <expand macro="citations"></expand> </tool>
--- a/tool_dependencies.xml Fri Sep 26 15:20:35 2014 -0400 +++ b/tool_dependencies.xml Tue Oct 13 12:55:05 2015 -0400 @@ -1,6 +1,6 @@ <?xml version="1.0"?> <tool_dependency> - <package name="samtools" version="1.1"> - <repository changeset_revision="c01b111a243b" name="package_samtools_1_1" owner="iuc" toolshed="https://testtoolshed.g2.bx.psu.edu" /> + <package name="samtools" version="1.2"> + <repository changeset_revision="192f00129358" name="package_samtools_1_2" owner="iuc" toolshed="https://testtoolshed.g2.bx.psu.edu" /> </package> </tool_dependency>