Mercurial > repos > devteam > lastz
annotate lastz_d.xml @ 7:86f2c3695694 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 8c6730485b00a47419ebe86278e4bd669377e3e2"
author | iuc |
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date | Sat, 27 Nov 2021 10:40:44 +0000 |
parents | ac0ffffa649e |
children | da6f44d06921 |
rev | line source |
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7
86f2c3695694
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 8c6730485b00a47419ebe86278e4bd669377e3e2"
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1 <tool id="lastz_d_wrapper" name="LASTZ_D" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@"> |
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8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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2 <description>: estimate substitution scores matrix</description> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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3 <macros> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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4 <import>lastz_macros.xml</import> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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5 </macros> |
7
86f2c3695694
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 8c6730485b00a47419ebe86278e4bd669377e3e2"
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6 <expand macro="bio_tools"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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7 <requirements> |
7
86f2c3695694
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 8c6730485b00a47419ebe86278e4bd669377e3e2"
iuc
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8 <requirement type="package" version="@TOOL_VERSION@">lastz</requirement> |
86f2c3695694
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 8c6730485b00a47419ebe86278e4bd669377e3e2"
iuc
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9 <requirement type="package" version="1.0.8">bzip2</requirement> |
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8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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10 </requirements> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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11 <command detect_errors="exit_code"><![CDATA[ |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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12 lastz_D |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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13 @TARGET_INPUT_COMMAND_LINE@ |
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c3767eaae954
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
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14 @query_input@ |
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8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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15 #if $score_file: |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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16 '--inferonly=${score_file}' |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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17 #else: |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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18 --inferonly |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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19 #end if |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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20 '--infscores=${output}' |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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21 ]]> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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22 </command> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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23 <inputs> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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24 <expand macro="target_input"/> |
5
ec4affe27298
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 25c49a61a5358cc7ab016fb5847328af7e67a24c"
iuc
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25 <param name="query" format="fasta,fastq,fasta.gz,fastq.gz,fastq.bz2" type="data" label="Select QUERY sequence(s)" help="These are the sequences that you are aligning against TARGET"/> |
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8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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26 <param name="score_file" type="data" format="txt" optional="true" label="Control file for inference" argument="--inferonly[=control_file]" help="Optional controf file. If nothing is selected, LASTZ_D uses default described in the manual"/> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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27 </inputs> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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28 <outputs> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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29 <data format="txt" name="output" label="${tool.name} on ${on_string}: substituion matrix"/> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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30 </outputs> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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31 <tests> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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32 <test> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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33 <param name="ref_source" value="history" /> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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34 <param name="target" value="chrM_human.fa" /> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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35 <param name="query" value="chrM_mouse.fa" /> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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36 <output name="output" value="lastz_d_test1.out" /> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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37 </test> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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38 <test> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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39 <param name="ref_source" value="history" /> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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40 <param name="target" value="chrM_human.fa" /> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
iuc
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41 <param name="query" value="chrM_mouse.fa" /> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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42 <param name="score_file" value="lastz_d_ctrl_file.txt" /> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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43 <output name="output" value="lastz_d_test2.out" /> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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44 </test> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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45 </tests> |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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46 |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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47 <help><![CDATA[ |
8e9252994649
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit c5379af63b23648020a4709f8ed9d9eac26582aa
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48 |
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49 **What is does** |
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50 |
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ec4affe27298
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 25c49a61a5358cc7ab016fb5847328af7e67a24c"
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51 LASTZ_D is a non-integer (**D** stands for Double) version of LASTZ that can be used to estimate substitution matrix that |
ec4affe27298
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 25c49a61a5358cc7ab016fb5847328af7e67a24c"
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52 will be used to score alignments. It was developed by `Bob Harris <http://www.bx.psu.edu/~rsharris/>`_ in the lab of |
ec4affe27298
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 25c49a61a5358cc7ab016fb5847328af7e67a24c"
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53 Webb Miller at Penn State as a part of LASTZ. Matrix computed by this tool is to be used by LASTZ (see below). |
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54 |
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55 .. class:: warningmark |
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56 |
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ec4affe27298
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 25c49a61a5358cc7ab016fb5847328af7e67a24c"
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57 **Read documentation** before proceeding. LASTZ is a complex tool with many parameter options. Fortunately, there is |
ec4affe27298
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 25c49a61a5358cc7ab016fb5847328af7e67a24c"
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58 a `great manual <https://lastz.github.io/lastz/>`_ maintained by its author. The two sections that are particularly |
ec4affe27298
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 25c49a61a5358cc7ab016fb5847328af7e67a24c"
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59 relevant to the inference of substitution matrix are |
ec4affe27298
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 25c49a61a5358cc7ab016fb5847328af7e67a24c"
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60 `Inferring Score Sets <https://www.bx.psu.edu/~rsharris/lastz/README.lastz-1.04.03.html#adv_inference>`_ and |
ec4affe27298
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 25c49a61a5358cc7ab016fb5847328af7e67a24c"
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61 `Inference Control File <https://www.bx.psu.edu/~rsharris/lastz/README.lastz-1.04.03.html#fmt_inference>`_. |
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62 |
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63 **Notes on the inference** |
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64 |
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ec4affe27298
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 25c49a61a5358cc7ab016fb5847328af7e67a24c"
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65 Inference is achieved by computing the probability of each of the 18 different alignment events (gap open, gap extend, and 16 substitutions). |
ec4affe27298
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 25c49a61a5358cc7ab016fb5847328af7e67a24c"
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66 These probabilities are estimated from alignments of the sequences. Of course, at first we don't have alignments, so the process |
ec4affe27298
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 25c49a61a5358cc7ab016fb5847328af7e67a24c"
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67 begins by using a generic scoring set to create alignments, infer scores from those, then realign, and so on, until the scores stabilize |
ec4affe27298
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 25c49a61a5358cc7ab016fb5847328af7e67a24c"
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68 or "converge". Ungapped alignments are performed until the substitution scores converge, then gapped alignments are performed |
ec4affe27298
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 25c49a61a5358cc7ab016fb5847328af7e67a24c"
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69 (holding the substitution scores constant) until the gap penalties converge. In the end you get a matrix like this:: |
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70 |
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71 # (a LASTZ scoring set, created by "LASTZ --infer") |
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72 |
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73 bad_score = X:-1781 # used for sub[X][*] and sub[*][X] |
0acd9701676b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a7e9d5b3906b7ebb35b1c29c3a8e8203b2cefccd
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74 fill_score = -178 # used when sub[*][*] not otherwise defined |
0acd9701676b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a7e9d5b3906b7ebb35b1c29c3a8e8203b2cefccd
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75 gap_open_penalty = 400 |
0acd9701676b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a7e9d5b3906b7ebb35b1c29c3a8e8203b2cefccd
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76 gap_extend_penalty = 30 |
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77 |
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78 A C G T |
0acd9701676b
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a7e9d5b3906b7ebb35b1c29c3a8e8203b2cefccd
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79 A 72 -79 -49 -97 |
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81 G -49 -178 100 -79 |
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83 |
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84 This dataset can then be used as an input to the **Read the substitution scores** parameter of LASTZ (Parameter section *Scoring*). |
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86 The iterative process can fail if there's not a lot of sequence to align. E.g. if after the 4th iteration there's nothing in the central 50% denominators go to zero and the process fails. |
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88 If the sequences you are aligning have GC content different than the usual ACGT 30-20-20-30 split, scoring inference should discover this and give you better alignments. |
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89 |
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90 |
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91 ]]> |
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92 </help> |
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93 <expand macro="citations"/> |
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94 </tool> |