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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/fastx_toolkit/fastx_trimmer commit fd099d17eceaa319fbfe429f4725328d88b18c9f
author iuc
date Thu, 10 Aug 2023 06:52:27 +0000
parents 2ff4f91ff564
children
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7
709c2debabb1 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/fastx_toolkit/fastx_trimmer commit fd099d17eceaa319fbfe429f4725328d88b18c9f
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1 <tool id="cshl_fastx_trimmer" version="1.0.2+galaxy@VERSION_SUFFIX@" profile="22.05" name="Trim sequences">
2
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2 <description></description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
6
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6 <expand macro="requirements">
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7 <requirement type="package" version="1.0.8">bzip2</requirement>
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8 </expand>
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9 <command detect_errors="exit_code"><![CDATA[
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10 @CATS@ fastx_trimmer -v
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11 -f $first
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12 -l $last
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13 @FQQUAL@
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14 @GZIP@
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15 > '$output'
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16 ]]></command>
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17 <inputs>
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18 <expand macro="fastx_input" />
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19 <param name="first" type="integer" value="1" label="First base to keep" />
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20 <param name="last" type="integer" value="21" label="Last base to keep" />
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21 </inputs>
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22 <outputs>
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23 <data name="output" format_source="input" metadata_source="input" />
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24 </outputs>
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25 <tests>
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26 <test>
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27 <!-- Trim a FASTA file - remove first four bases (e.g. a barcode) -->
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28 <param name="input" value="fastx_trimmer1.fasta" />
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29 <param name="first" value="5"/>
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30 <param name="last" value="36"/>
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31 <output name="output" ftype="fasta" file="fastx_trimmer1.out" />
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32 </test>
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33 <test>
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34 <!-- Trim a FASTQ file - remove last 9 bases (e.g. keep only miRNA length sequences) -->
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35 <param name="input" value="fastx_trimmer2.fastq" ftype="fastqsolexa"/>
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36 <param name="first" value="1"/>
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37 <param name="last" value="27"/>
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38 <output name="output" ftype="fastqsolexa" file="fastx_trimmer2.out" />
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39 </test>
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40 <test>
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41 <!-- Trim a FASTQ.gz file and output FASTQ.gz -->
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42 <param name="input" value="fastx_trimmer2.fastq.gz" ftype="fastqsanger.gz"/>
748363ac106b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/fastx_toolkit/fastx_trimmer commit f3f313cb59922b945ac9587ff718b231d0f8db5c
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43 <param name="first" value="1"/>
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44 <param name="last" value="27"/>
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45 <output name="output" ftype="fastqsanger.gz" decompress="true" file="fastx_trimmer2.out.gz" />
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46 </test>
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47 <test>
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48 <!-- Trim a FASTQ.bz2 file and output FASTQ.bz2 -->
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49 <param name="input" value="fastx_trimmer2.fastq.bz2" ftype="fastqsanger.bz2"/>
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50 <param name="first" value="1"/>
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51 <param name="last" value="27"/>
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52 <output name="output" ftype="fastqsanger.bz2" decompress="true" file="fastx_trimmer2.out.bz2" />
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53 </test>
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54 </tests>
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55 <help><![CDATA[
0
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56 **What it does**
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57
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58 This tool trims (cut bases from) sequences in a FASTA/Q file.
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59
0
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60 --------
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61
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62 **Example**
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63
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64 Input Fasta file (with 36 bases in each sequences)::
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65
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66 >1-1
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67 TATGGTCAGAAACCATATGCAGAGCCTGTAGGCACC
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68 >2-1
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69 CAGCGAGGCTTTAATGCCATTTGGCTGTAGGCACCA
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70
0
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71 Trimming with First=1 and Last=21, we get a FASTA file with 21 bases in each sequences (starting from the first base)::
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72
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73 >1-1
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74 TATGGTCAGAAACCATATGCA
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75 >2-1
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76 CAGCGAGGCTTTAATGCCATT
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77
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78 Trimming with First=6 and Last=10, will generate a FASTA file with 5 bases (bases 6,7,8,9,10) in each sequences::
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79
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80 >1-1
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81 TCAGA
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82 >2-1
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83 AGGCT
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84
0
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85 ------
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86
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87 This tool is based on `FASTX-toolkit`__ by Assaf Gordon.
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88
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89 .. __: http://hannonlab.cshl.edu/fastx_toolkit/
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90 ]]></help>
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91 <expand macro="citations" />
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92 <!-- FASTX-Trimmer is part of the FASTX-toolkit, by A.Gordon (gordon@cshl.edu) -->
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93 </tool>